Hair composition based on plant extracts

Abstract

The invention relates to a hair composition for skin and follicular penetration, characterized in that it comprises: (A) from 45% to 95% by weight of exosomes derived from plant stem cells, (B) from 2% to 40% by weight of an extract of the fruit of the palm tree of the genus Serenoa, (C) from 1% to 15% by weight of an extract of the hairy roots of Ocimum Basilicum, (D) from 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E), as well as its use in the prevention or reduction of hair loss, the cosmetic use of the composition for increasing hair density, or increasing hair diameter, or a cosmetic hair treatment method comprising the application of the composition on the scalp, possibly followed by a scalp massage.

Description

Title of the invention: Hair composition based on plant extracts Technical field

[0001] The present invention relates to the field of hair treatment.

[0002] It relates more particularly to a hair composition based on plant extracts making it possible to increase hair density, to increase the diameter of the hair or even to prevent or reduce hair loss. Prior art

[0003] Hair grows from follicles located in the scalp.

[0004] Structure and development of the hair follicle:

[0005] The hair follicle is composed of a diverse assembly of approximately 20 distinct cell types, each with specialized functions. The hair follicle regulates hair growth through a complex interaction between hormones, neuropeptides, and immune cells. This complex interaction induces the hair follicle to produce different types of hair as observed on different parts of the body. Hair follicles emerge through a dynamic interaction between the dermis and epidermis, orchestrated by a sequence of cellular interactions: - Dermo-epidermal interaction: Hair follicle development begins with the interaction between the dermal and epidermal layers of the skin. An initial signal from the epidermis triggers this process. - Fibroblast activation: In response to the epidermal signal, fibroblasts, specialized cells in the dermis, become activated. They organize and transmit signals to neighboring keratinocytes. - Formation of the epidermal plaque: Activated keratinocytes react by forming an epidermal plaque. This structure begins to grow downward, penetrating the dermal layer. - Formation of the primary bud: As the epidermal plate extends into the dermis, it forms a primary bud. This primary bud serves as the fundamental structure for the future hair follicle. - Formation of the dermal papilla: the primary bud generates signals that act as messages, influencing the surrounding fibroblasts. These messages lead to the stabilization and organization of the fibroblasts, giving rise to the dermal papilla. - Differentiation into a hair follicle: Over time, the primary bud, under the continuous influence of signals from the dermal papilla, undergoes gradual differentiation. This process ultimately results in the formation of a fully functional hair follicle.

[0006] In summary, hair follicle development is a finely orchestrated process, driven by a cascade of signaling events between the epidermis and dermis, involving fibroblasts, keratinocytes, and the formation of key structures such as the epidermal plate and dermal papilla.

[0007] As in many systems of the human body, the chemical pathways and precise roles involved in hair growth are not fully understood. However, it is known that hair growth is facilitated by the physical interaction between the dermis and epidermis within the dermal papilla, where keratinocytes and fibroblasts are condensed. The dermal papilla is an area particularly rich in collagens and glycosaminoglycans that maintain close contact between the two cell populations and promote the chemical communication necessary for hair follicle growth. Type IV collagen and laminin 5 are important proteoglycans because they constitute the basement membrane, the attachment zone between the epidermis and dermis, and, in the case of hair, between the root sheath and the dermis.

[0008] A hair follicle is composed of: - The papilla is a large structure located at the base of the hair follicle. The papilla is primarily made up of connective tissue and a capillary loop. Cell division in the papilla is either rare or nonexistent. - Around the papilla is the hair matrix. - A root sheath composed of an outer and inner root sheath. The outer root sheath appears empty with cuboidal cells when stained with H&E stain. The inner root sheath is composed of three layers: Henle's layer, Huxley's layer, and an inner cuticle that is continuous with the outermost layer of the hair fiber. - The protrusion is located in the external root sheath where the arrector pili muscle inserts. It houses several types of stem cells, which supply the entire hair follicle with new cells and participate in the healing of the epidermis after injury.

[0009] Other structures associated with the hair follicle include the cup into which the follicle grows, called the infundibulum, the arrector pili muscles, the sebaceous glands, and the apocrine sweat glands. Receptors in the hair follicle detect the position of the hair.

[0010] Attached to the follicle is a small bundle of muscle fibers called the arrector pili muscle. This muscle is responsible for straightening the hair follicle more perpendicular to the skin's surface, thus causing the follicle to protrude slightly above the surrounding skin (piloerection) and a pore surrounded by skin sebum. This process results in the formation of goosebumps.

[0011] Also attached to the follicle is a sebaceous gland, which produces the oily or waxy substance called sebum. The higher the hair density, the more sebaceous glands there are.

[0012] Hair structure and growth:

[0013] On the outside of the hair, flat, scale-like cells form the cuticle, while on the Inside, the cortex is composed of elongated cells which, in turn, are made up of an organized amalgam of sulfur-rich proteins adopting an α-helical structure. Keratin, which constitutes 50% of hair protein, is the protein responsible for the shape and strength of hair. It is mainly composed of glycine, leucine, and cysteine.

[0014] The hair shaft is the visible part of the hair, it originates near the infundibulum, at the opening of the sebaceous canal. In adults, it has a diameter varying from 70 to 100 qm and is composed of three well-defined layers.

[0015] The medulla, also called the medulla, which occupies the central part of the hair shaft. The medulla is made up of large, anucleate, lightly pigmented cells.

[0016] The cortex plays an essential role within the hair, representing approximately 90% of its total weight. It is made up of tightly knit keratinized spindle cells, forming a solid network. Inside the cortex are located the melanocyte pigments, which determine the hair color.

[0017] The cuticle is the outermost layer of the hair shaft. It is composed of keratinized cells called "scales" that are held together by a lipid binder. These scales fit together like roof tiles, with their free edges directed toward the end of the hair. Their main function is to protect the cortex from the multiple aggressions to which the hair is likely to be exposed.

[0018] Hair growth occurs in cycles and includes four phases: - The Anagen phase is the longest phase of the cycle, lasting approximately 2 to 5 years, and is the phase during which hair actively grows. This stage accounts for 85% to 90% of all hair. It can be disrupted by various factors, including nutritional deficiencies, stress, and genetic predispositions. - The Catagen phase is a short transitional stage that begins at the end of the Anagen phase and lasts approximately 2 to 3 weeks. This phase accounts for 5% of all hair, during which hair growth stops. Various factors, including mineral deficiencies, blood circulation, and oxygen supply insufficient, as well as genetic predispositions, can disrupt the Catagen phase. - The Telogen phase is the resting phase and typically accounts for 6% to 8% of all hair. This phase lasts approximately 100 days, during which the hair follicle is resting and the telogen phase hair is fully formed. - The Exogenous phase is the last phase of the hair cycle. This phase involves the growth of new hair in the follicles and the beginning of the shedding of old hair.

[0019] The length of a cycle varies considerably, with an average of between 3 and 5 years. This period depends on several factors, such as the sex of the individual, as well as the nature and specific location of the hair follicle.

[0020] [Tables 1] Anagen phase Telogen phase Duration (months) Proportion (%) Duration (months) Proportion (%) Hair 24-72 80-90 3-6 10-15 Eyebrows 1-2 10 3 90 Pubic hair 4-8 30 12 70 Arms 3-12 20 3-12 80

[0021] The duration of the phases of the hair cycle varies depending on the location of the hair follicles.

[0022] Each hair follicle goes through about 20 to 30 distinct cycles during its life, and its development is autonomous and independent of the others.

[0023] Hair loss:

[0024] Hair loss can be attributed to a wide range of factors, such as genetics, disease, nutritional deficiencies, aging, hormonal changes, and stress, which result in alterations in hair follicle cycles, including a shortening of the anagen phase, an increase in the number of hair follicles in the telogen phase, and changes in hair follicle morphology.

[0025] The most common type of hair loss is androgenic alopecia, also known as male pattern baldness. This condition affects millions of people, both men and women. Recently, the incidence of hair loss has increased significantly among young people. Male pattern baldness is usually caused by the conversion of testosterone to dihydrotestosterone, which reduces the size of hair follicles and the blood vessels that support them, eventually by causing some follicles to close or even die. The transformation of testosterone into dihydrotestosterone is caused by the hormone 5a-reductase. It is also known that vitamin H (biotin) deficiencies can lead to thinning hair.

[0026] It has been suggested that effective strategies to combat hair loss include prolonging the anagen phase, stimulating the telogen-anagen transition of follicles, reversing miniaturization, and enhancing the viability of dermal papilla cells to stimulate the generation of new follicles.

[0027] Minoxidil (MNX) and finasteride are currently hair loss treatments for patients with alopecia in many countries. However, their therapeutic benefits are limited due to their temporary and adverse effects.

[0028] Nevertheless, consumers are increasingly looking for effective natural alternatives, particularly based on plant extracts, capable of treating hair loss or increasing hair density or diameter. Summary

[0029] The invention relates to a hair composition for cutaneous and follicular penetration characterized in that it comprises: (A) from 45% to 95% by weight of exosomes from plant stem cells, (B) from 2% to 40% by weight of an extract of the fruit of the palm tree of the genus Serenoa, (C) from 1% to 15% by weight of an extract of the hairy root culture of Ocimum Basilicum, (D) from 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0030] Another aspect relates to the composition according to the invention for its use in the prevention or reduction of hair loss.

[0031] Another aspect concerns the cosmetic use of the composition according to the invention to increase hair density, or increase the diameter of the hair.

[0032] Another aspect relates to a cosmetic hair treatment method comprising the application of the composition according to the invention to the scalp, optionally followed by a massage or friction of the scalp.

[0033] Without wishing to be bound by any theory, exosomes derived from plant stem cells appear to act as vesicular vectors allowing the active ingredients of the composition to be transported to the papilla of the hair follicles. The small size and the liposomal envelope of these exosomes gives them a capacity to optimal penetration through the scalp and pilosebaceous orifices. Thanks to the protection provided by their lipid membrane, they also help preserve these active ingredients when applied to the scalp by friction.

[0034] The composition according to the invention comprises a mixture of active ingredients advantageously chosen for their beneficial effects on the development of the hair follicle: - Reduction of dihydrotestosterone (DHT), a hormone associated with hair loss, by inhibiting 5α-reductase activity. This inhibition helps preserve the health and longevity of hair follicles. - Improved blood circulation to the dermis, the deeper layer of the skin, which houses the hair follicles. Improved circulation helps deliver essential nutrients and oxygen to the hair follicles, promoting their growth and vitality. - Optimal rooting of hair into the skin, which helps prevent premature hair loss and promotes the maintenance of healthy hair. - Healthy scalp: The composition according to the invention promotes hydration of the scalp, helps maintain the perfect moisture balance, creating an ideal environment for healthy hair growth.

[0035] The exosomes derived from plant stem cells and the active ingredients act synergistically to give the composition excellent skin and follicular penetration, excellent tolerance and efficacy that goes beyond current treatments.

[0036] The composition according to the invention makes it possible to promote hair growth, increase hair density and / or thickness, increase hair strength, reduce scalp inflammation and protect it from environmental stress factors, thus promoting a healthier environment more conducive to hair growth.

[0037] The composition according to the invention also has the advantage of being very stable during storage and of not degrading during handling. This is a very important advantage given that the integrity of the membrane of plant-derived exosome-type nanoparticles is crucial for their bioactivity.

[0038] The features set out in the following paragraphs may, optionally, be implemented, independently of one another or in combination with one another: Brief description of the figures

[0039] Other characteristics, details and advantages will appear on reading the detailed description below, and on analyzing the attached drawings, in which:

[0040] [Fig.l]

[0041] [Fig.l] shows the evaluation of hair density by measuring the number of hairs per cm2 on 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test).

[0042] [Fig.2]

[0043] [Fig.2] shows the evaluation of the regrowth rate in 48 hours on 1cm2 of shaved scalp (values ​​expressed as median ± SE; / ?<0.05 by t-test).

[0044] [Fig.3]

[0045] [Fig.3] shows the evaluation of the rate of anagen and telogen hair in % on 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test).

[0046] [Fig.4]

[0047] [Fig.4] shows the evaluation of the density of terminal and hairy hair per cm2 on 1cm2 of shaved scalp (values ​​expressed as median ± SE; p<0.05 by t-test). Detailed description

[0048] The hair composition according to the invention is characterized in that it comprises the following ingredients of plant origin: (A) from 45% to 95% by weight of exosomes from plant stem cells, (B) from 2% to 40% by weight of an extract of fruit of the palm tree of the genus Serenoa, (C) from 1% to 15% by weight of an extract of hairy root culture of Ocimum Basilicum, (D) from 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of Rosemary essential oil, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0049] (A) Exosomes from plant stem cells:

[0050] Plant exosomes are nanoparticles produced by the multi-vesicular corpuscles of the majority of plant or fruit stem cells, measuring between 30-200nm. They are extruded from the stem cells from which they originate, into the extracellular matrix where they fulfill the role of messengers allowing communication, intercellular signaling and the transport of biomaterials participating in the regulation of the expression of cellular functions. Their native cargo is composed of various proteins and RNAs forming the cytosol, surrounded by a protective lipid membrane. Many extraction methods have been developed in recent years, each with its advantages and disadvantages; the most efficient being those which allow to obtain a high purity of the vesicles while maintaining their integrity.

[0051] The principle of extracting exosomes is composed of sequential steps where ultracentrifugation and ultrafiltration play a major role because they are adapted to the nano-size, shape and density of these extracellular vesicles.

[0052] Ultracentrifugation is a gold standard in the production of plant exosomes. It is a differential centrifugation, based on the separation of particles according to their density, size and shape using a series of high-speed centrifugations in a homogeneous medium such as a phosphate saline solution, preceded by an initial preparation of the sample to remove cells and larger debris.

[0053] Further purification is often necessary to remove unwanted debris such as certain proteins, RNA and membrane aggregates, which can be achieved by gradient ultracentrifugation.

[0054] The following ultrafiltration is a method isolating exosomes based on their size, molecular weight and hydrodynamic diameter through a porous rectangular channel where smaller particles are again dissociated from larger ones.

[0055] According to one embodiment, the exosomes derived from plant stem cells are extracted in the following manner: (a) Preparation of plant stem cell lines in the laboratory, (b) Stem cell culture and amplification, (c) Observation of their differentiation by electron microscopy, (d) Series of progressive ultracentrifugations, (e) Separation of the particles obtained by successive ultrafiltrations in order to obtain high-purity exosomes and, (f) High pressure homogenization of the obtained material, extraction of the vesicles and liposomal stabilization of their contents.

[0056] Plant-derived nanoparticles obtained by these processes have the potential to be stable and resistant to the scalp microenvironment due to the lipids contained in their membrane and to transport the active ingredients through the stratum corneum. The processes used also give the formulations significant stability that does not degrade during handling and storage of the final cosmetic product preparations.

[0057] The exosomes derived from plant stem cells can be chosen from exosomes derived from stem cells of flowering plants, in particular from the following families: - Curcubitaceae, in particular of the Lagenaria genus, such as Lagenaria siceraria, of the Sicyos genus, such as Sicyos angulatus, or of the Benincasa genus, such as Benincasa hispida; - Rosacea e, notably of the genus Malus, such as Malus domestica, of the genus Prunus, such as Prunus armeniaca or Prunus dulcis, or of the genus Rosa, such as Rosa gallica or Rosa canina.

[0058] It is possible to use mixtures of exosomes.

[0059] The content of exosomes derived from plant stem cells in the hair composition according to the invention is from 45% to 95% by weight, in particular from 60% to 94% by weight, in particular from 75% to 93% by weight, more particularly from 85% to 92% by weight, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0060] (B) Extract of fruit of the palm tree of the genus Ser enoa:

[0061] The genus Serenoa includes a single species of the Arecaceae family, Serenoa repens (heterotypic synonym: Serenoa serrulala) which is native to the southeastern United States, particularly Florida and its subtropical climate. It is a species of dwarf palm that can reach 3 meters in height and is better known as "cabbage palm" or "Florida fan palm". Its fruits are globose-ellipsoid drupes, green then orange and finally black when ripe.

[0062] The fruit extract of this palm tree is used in the composition according to the invention for its ability to block 5a-reductase, which leads to a decrease in DHT levels. This botanical extract plays an essential role in solving the problem of hair loss.

[0063] The content of the fruit extract of the palm tree of the genus Serenoa in the hair composition according to the invention is from 2% to 40% by weight, in particular from 53% to 930% by weight, in particular from 64% to 208% by weight, more particularly from 6.5% to 7.510% by weight, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0064] (C) Ocimum Basilicum hairy root culture extract:

[0065] Basil is the common name for the culinary herb Ocimum basilicum.

[0066] The hairy root culture extract of Ocimum Basilicum can be obtained by infecting a small portion of a basil plant with the naturally occurring soil bacterium Agrobacterium rhizogenes, followed by cultivation on an agar plate. This infection leads to a natural transformation of the plant's cells, which can then form tiny roots, called hairy roots. These hairy roots can be propagated on artificial culture media in Petri dishes or suspended in liquid media. After producing sufficient biomass, the roots are harvested, and the active compounds are extracted and dissolved in a solvent to make them available for hair treatment formulations.

[0067] Ocimum Basilicum hairy root culture extract offers several key benefits in combating hair loss, including: - Inhibition of 5α-reductase II activity: One of the central mechanisms by which Ocimum Basilicum contributes to hair health is by effectively inhibiting the activity of 5α-reductase II. This inhibition is crucial in reducing the conversion of testosterone to dihydrotestosterone (DHT), a hormone strongly associated with hair loss. By decreasing DHT levels, Ocimum Basilicum helps maintain the health of hair follicles and can potentially slow or prevent hair loss. - Stimulation of dermal papilla cell proliferation: Another significant benefit of Ocimum Basilicum is its ability to enhance the proliferation of dermal papilla cells within hair follicles. Dermal papilla cells play a crucial role in regulating hair growth, and their increased proliferation can lead to stronger and healthier hair follicles, promoting hair growth and thickness.

[0068] The content of the hair root culture extract of Ocimum Basilicum in the hair composition according to the invention is from 1% to 15% by weight, from 1% to 10% by weight, in particular from 1.5% to 7% by weight, more particularly from 1.5% to 4% by weight, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0069] (D) Niacinamide:

[0070] Niacinamide (also called nicotinamide) is one of three forms that vitamin B3 can take, the other two being niacin and nicotinamide riboside. It produces multiple effects as follows: - Keratin synthesis: Niacinamide plays a crucial role in the synthesis of keratin, a protein that forms the structural basis of hair. By promoting keratin production, it contributes to the overall health and strength of hair strands. - Anti-inflammatory properties: The anti-inflammatory properties of niacinamide are beneficial in soothing and calming the scalp. This can relieve discomfort and irritation often associated with various scalp conditions, thus promoting a healthier environment for hair growth. - Improved blood circulation: Niacinamide improves blood circulation, which is essential for delivering essential nutrients and oxygen to hair follicles. Improved circulation can stimulate follicles and promote healthier hair growth. - Reduced redness: By reducing redness and irritation of the scalp, niacinamide helps create a more comfortable and conducive environment for hair follicles, which can potentially reduce hair loss. - Promotes hair growth: Niacinamide is linked to promoting hair growth by nourishing hair follicles and stimulating their activity. This can lead not only to increased hair growth, but also to thicker, healthier strands. - Reduced hair loss: Niacinamide's ability to support hair follicles and reduce inflammation may contribute to a decrease in hair loss, helping to maintain a thicker head of hair. - Improved hair thickness: With continued use, niacinamide may contribute to thicker, stronger hair. - Moisture Retention: Niacinamide helps retain moisture, preventing dryness and brittleness. Hydrated hair is less prone to damage and breakage. - Environmental protection: Niacinamide acts as a protective shield against environmental damage, such as pollution and UV rays. This helps maintain the integrity of hair and prevents it from becoming brittle and fragile.

[0071] The niacinamide content in the hair composition according to the invention is 0.05% to 10% by weight, in particular 0.1% to 8% by weight, in particular 0.2% to 5% by weight, more particularly 0.3% to 1% by weight, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0072] (E) Rosemary essential oil:

[0073] Rosemary, Salvia rosmarinus, is a species of shrub in the Lamiaceae (or Labiatae) family, growing wild around the Mediterranean, particularly in arid, rocky scrubland on calcareous soils4.

[0074] Rosemary essential oil has multiple properties. It is incorporated into the composition according to the invention mainly to produce the following two effects: - Vasodilatory properties: Rosemary essential oil has impressive vasodilatory properties, meaning it helps dilate blood vessels. This action can improve blood circulation in the scalp, ensuring that hair follicles receive an adequate supply of nutrients and oxygen. Improved circulation can stimulate hair follicles, which can promote healthier hair growth. - Reduction of 5a-reductase: Another significant benefit of rosemary essential oil is its ability to reduce the activity of 5a-reductase. By inhibiting this An enzyme, it helps reduce levels of dihydrotestosterone (DHT), a hormone strongly linked to hair loss. This reduction in DHT levels can help maintain healthy hair follicles and potentially slow or prevent hair loss.

[0075] By synergistically exploiting these properties, the inclusion of rosemary essential oil in the hair composition according to the invention offers a holistic approach to treating hair loss. It not only promotes better blood circulation to nourish the hair follicles, but it also addresses the root cause of hair loss by reducing DHT levels, thereby potentially offering individuals a comprehensive solution for the maintenance and regeneration of their hair.

[0076] The content of rosemary essential oil in the hair composition according to the invention is 0.05% to 10% by weight, in particular 0.08% to 8% by weight, in particular 0.1% to 5% by weight, more particularly 0.15% to 1% by weight, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

[0077] Other ingredients:

[0078] The hair composition according to the invention may comprise a physiologically acceptable liquid medium, such as an aqueous, alcoholic or hydroalcoholic solvent.

[0079] The alcoholic solvent is chosen for example from lower alcohols containing from 1 to 8 carbon atoms and in particular 1 to 6 carbon atoms, such as ethanol, isopropanol, propanol, butanol; polyethylene glycols containing from 6 to 80 ethylene oxides; polyols such as propylene glycol, isoprene glycol, butylene glycol, glycerin, sorbitol, 1,3-propanediol.

[0080] When the composition comprises an aqueous solvent, it has a pH compatible with the skin, preferably ranging from 3 to 8 and better still from 4 to 7.

[0081] When the composition comprises an aqueous or hydroalcoholic medium, it is possible to add a fatty (or oily) phase to this medium.

[0082] The hair composition according to the invention is in particular a composition intended for topical application to the scalp and / or hair.

[0083] The composition according to the invention may also comprise one or more additional compounds chosen in particular from a vitamin, a humectant or moisturizing agent, a conditioner, a vasodilator agent (eg essential oil of Mentha piperitd), an antiseptic agent, an antimicrobial agent, an antioxidant agent (eg Faex extract) and / or a pH regulator, an emulsifying agent, a surfactant, in particular chosen from non-ionic, anionic, cationic and amphoteric surfactants, a gelling agent, a viscosity control agent, polymeric or not, natural or synthetic, a bulking agent, a coloring agent and / or perfuming agent, a UV filter, a filler such as pearls, titanium dioxide, resins and clays, a peptizer, an acidic agent, an alkaline agent, a reducing agent, an oxidizing agent, an additional active agent different from the aforementioned active agents intended to further improve the activity on hair loss, regrowth and / or on slowing down hair loss, and having already been described for this activity, such as nicotinic acid esters, including in particular tocopherol nicotinate, benzyl nicotinate and C1-C6 alkyl nicotinates such as methyl or hexyl nicotinates; hair regrowth promoting agents selected from pyrimidine 3-oxide derivatives such as 2,4-diaminopyrimidine-3-N-oxide (Aminexil), pyridine-dicarboxylate derivatives or one of its salts such as pyridine-2,4-dicarboxylate dimethyl ester; and a mixture of these compounds.

[0084] The vitamin can be chosen from group B such as: B1, B2, B5 (panthenol), B6, B7, B8, B12, a vitamin C, a vitamin E, or one of their mixtures.

[0085] The humectant or moisturizing agent may be chosen from: glycerin, aloe vera, soybean germ extract, glycosaminoglycan, retinyl palmitate, ferric sulfate or one of their mixtures.

[0086] The conditioner can be chosen from: hydrolyzed keratin, coconut extract, sunflower seed oil.

[0087] The above additional compounds may each be present in an amount varying from 0.000001% to 20% by weight relative to the total weight of the composition.

[0088] The composition according to the invention can be presented in all the galenic forms normally used in the cosmetic field, such as a lotion, a serum, a shampoo, a conditioner, a dry shampoo, a gel, a wax, a lacquer, a spray, a mousse, a paste, a cream, a powder, an oil, a mask, a treatment, or a tonic.

[0089] It can be formulated in the form of an aqueous or hydro-alcoholic solution or suspension, an emulsion or dispersion of liquid or semi-liquid consistency obtained by dispersing a fatty phase in an aqueous phase (O / W) or vice versa (W / O), a dispersion or emulsion of soft consistency, an aqueous or hydro-alcoholic gel, or even microcapsules or microparticles, or vesicular dispersions of ionic and / or non-ionic type.

[0090] According to a preferred embodiment, the composition is in the form of a lotion or serum.

[0091] Applications:

[0092] The composition according to the invention is preferably applied to the scalp. After this application, the scalp can be massaged or rubbed to promote penetration of the active ingredients of the composition through of the stratum corneum. The application time can vary from 1 minute to 30 minutes. The composition can then be rinsed off depending on the form it takes. For example, in the case of a dry shampoo, the composition does not require rinsing.

[0093] The present invention also relates to the composition as described above for its use in the prevention or reduction of hair loss, the application being optionally followed by a massage of the scalp, then optionally rinsing.

[0094] The present invention also relates to the cosmetic use of the composition as described above to increase hair density, or increase the diameter of the hair.

[0095] The present invention also relates to a cosmetic hair treatment method comprising the application of the hair composition as described above to the scalp, the application optionally being followed by a massage of the scalp, then optionally rinsing. Examples

[0096] 1. Serum according to the invention

[0097] The INCI ingredients of the serum according to the invention and their percentages by weight are presented in Table 1 below.

[0098] The preparation of the serum according to the invention is carried out at a constant temperature of 20°C and comprises 5 main phases (cp) described in Table 2 below.

[0099] [Tables 1] Ingredients % by weight Water 87.684558 Malus Domestica Fruit Cell Culture Extract 0.200000 Serenoa Serrulata Fruit Extract 0.015000 Ocimum Basilicum Hairy Root Culture Extract 0.004500 Niacinamide 0.000750 Rosmarinus Officinalis Extract 0.000400 Glycerin 9.610000 Aloe Barbadensis Leaf Juice Powder 0.870000 Phenoxyethanol 0.536000 Isopropyl Alcohol 0.300000 Ethanol 0.150000 Faex Extract 0.150000 Glycine Soja Germ Extract 0.090000 Hydrolyzed Keratin 0.060000 PEG-40 Hydrogenated Castor Oil 0.060000 Lecithin 0.055000 Rosmarinus Officinalis Leaf Oil 0.055000 Cocos Nucifera Fruit Extract 0.050000 Mentha Piperita Oil 0.035000 Xanthan Gum 0.020000 Tocopheryl Acetate 0.011050 Sodium Benzoate 0.009000 Sodium Citrate 0.008850 Ascorbic Acid 0.005050 Citric Acid 0.005050 Ascorbyl Palmitate 0.003000 Glycosaminoglycans 0.003000 Potassium Sorbate 0.003000 Retinyl Palmitate 0.003000 Helianthus Annuus Seed Oil 0.000600 Panthenol 0.000600 Biotin 0.000300 Inositol 0.000300 Pyridoxine HCl 0.000300 Thiamine HCl 0.000300 Calcium Sulfate 0.000180 Riboflavin 0.000045 Magnesium Sulfate 0.000039 Manganese Sulfate 0.000039 Copper Sulfate 0.000030 Cyanocobalamin 0.000030 Potassium Sulfate 0.000015

[0100] [Tables2] 0 Procedure Ingredients A Homogenize A Aloe Barbadensis Leaf Juice Powder Water B Make a homogeneous premix and add B Glycerin Faex Extract, Glycine Soja Germ Extract, Hydrolyzed Keratin, Tocopheryl Acetate, Glycosaminoglycans, Retinyl Palmitate, Ascorbyl Palmitate, Niacinamide, Panthenol, Riboflavin, Biotin, Inositol, Thiamine HCl, Pyridoxine H Cl, Cyanocobalamin, Calcium Sulfate, Magnesium Sulfate, Copper Sulfate, Potassium Sulfate, Zinc Sulfate, Manganese Sulfate, Ferric Sulfate. C Add C Serenoa Serrulata Fruit Extract D Make a homogeneous premix and add D Rosmarinus Officinalis Leaf Oil Mentha Piperita Oil Glycerin, Water, Lecithin, Cocos Nucifera Fruit Extract, Sodium Citrate, Ascorbic Acid, Citric Acid, Tocopheryl Acetate, Helianthus Annuus Seed Oil, Rosmarinus Officinalis Extract.E Add E Ocimum Basilicum Hairy Root Culture Extract Malus Domestica Fruit Cell Culture Extract Phenoxyethanol .

[0101] 2. Randomized, double-blind, placebo-controlled clinical evaluation of efficacy serum according to example 1).

[0102] 2.1. Objective: This clinical study aims to evaluate the efficacy and safety of the serum according to Example 1 in reducing hair loss, stimulating hair regrowth and improving hair health after 12 weeks of use. The evaluation of the impact of this intervention is measured quantitatively and at the level of each participating subject.

[0103] 2.2. Methodology of the clinical study: 20 men and 20 women are selected, 40 participants in total, and randomly divided into two groups identical in terms of sex and average age of the participants. The selection is made according to the following predefined criteria and evaluated by an expert for their degree of hair loss on scales recognized by the scientific community.

[0104] 2.2.1. Inclusion criteria: participants of both sexes aged 25 to 55 years with signs of mild to moderate hair loss rated II-III on the Ludwig scale for women (Dermatology (4 ed.). Elsevier. 2018. pp. 2637-2648) and III-IV on the Norwood-Hamilton scale for men (Guarrera M, Cardo P, Arrigo P, Rebora A, “Reliability of hamilton-norwood classification”, Int J Trichology, vol. 1, no. 2, 2009, pp. 120-2).

[0105] 2.2.2. Exclusion criteria: individuals undergoing medical or other treatment for the hair loss, individuals on hormone therapy.

[0106] 2.2.3. Distribution by age group:

[0107] Group A: subjects receiving the serum (25-35 years: 5 men, 6 women; 35-55 years: 5 men, 4 women).

[0108] Group B: subjects receiving placebo (25-35 years: 5 men, 6 women; 35-55 years: 5 men, 4 women).

[0109] 2.2.4. Distribution by degree of baldness

[0110] Initial assessment of participants according to severity of hair loss and taking into account age group [YES] [Tables3] Scales Group A Group B Ludwig II 4 4 Ludwig III 6 6 Norwood-Hamilton III 3 3 Norwood-Hamilton IV 7 7

[0112] 2.2.5. Description of evaluation criteria and tools

[0113] The baseline and end-of-study measurements are defined by the following criteria: - Counting hair lost at 30, 60 and 90 days; - Evaluation of hair density; - Hair diameter analysis; - Specialist assessment of the degree of hair loss on international classification scales; - Self-assessment of subjects using a satisfaction questionnaire with targeted responses (“closed-ended questionnaire”).

[0114] These measurements are taken using the following devices:

[0115] Hair loss was assessed using the SELS® (Surface Evaluation of the Living Skin) counting method developed by Professor Tronnier at the Institute of Experimental Dermatology at the University of Witten-Herdecke (Germany).

[0116] Hair photography analyses were carried out using a Visioscan® VC 20plus device, which allows for precise measurement of hair density, hair diameter and degree of hair loss. pH, hydration and transepidermal water loss analyses were carried out using the C+K Multi Probe device and its various probes, detailed below.

[0117] 2. 2. 5.a. Evaluation by Visioscan ® VC 20plus

[0118] The Visioscan® VC 20plus is a device using the SELS® evaluation method that allowed us to capture high-resolution images to characterize skin structures, accurately measure the number, length and diameter of hairs in a shaved area and the level of hydration / dryness / scaling of the scalp as well as the sebum production of each sebaceous gland in the area studied.

[0119] Technical characteristics: Image size: 10 x 8 mm; Resolution: 1 / 2 B / W CMOS-sensor 1.3MPix(1280xl024pixels); Light source: UV-A-LED 390nm; Lens 20mm; f / 2.8

[0120] 2. 2. 5.b. Evaluation by C+K Multi Probe Adapter System

[0121] This platform allows for more specific analyses using the Skin-pH-Meter PH 905, Corneometer® Cm 825 and Tewameter® TM Nano probes for reproducible results.

[0122] 2. 2. 5.c. Evaluation by the Skin-pH- Meter PH 905

[0123] The Skin-pH-Meter PH 905 allowed us to take pH measurements on the surface of the scalp and to establish that it was healthier after using our serum by modifying the skin microbiome. The measurement principle is based on the application to the test area of ​​two combined electrodes, one sensitive to the H+ ion, the other serving as a reference, connected to a handpiece containing the electronic measuring instruments.

[0124] 2. 2. 5.d. Evaluation by Corneometer ® Cm 825

[0125] The Corneometer® Cm 825 indicates the level of hydration of the superficial layers of the Stratum corneum at the level of the scalp by measuring its electrical properties, the measurement principle being based on capacitance measurements of the dielectric medium. Any change in hydration of the skin surface causes a modification of these dielectric constants, and consequently of the capacitance measurement.

[0126] The probe is applied vertically to the scalp. During the measurement, an electric field penetrates the superficial layers of the skin and the dielectric constant is measured. The measurement is expressed in units from 0 to 125 according to the following indices: - 6-10: very dry scalp; - 10-50: dry scalp; - 50-125: hydrated scalp.

[0127] 2. 2. 5.e. Evaluation by Tewameter ® TM Nano

[0128] The Tewameter® TM Nano is a high-precision measuring device since it can analyze transepidermal water loss expressed in g / h / m2 in areas as small as 2mm in diameter. The evaporation of water through the skin is an essential element for the proper functioning of the metabolism.

[0129] 2.3. Data analysis

[0130] The data and images obtained are automatically captured by the professional TrichoSciencePro software. This program integrates the diagnostic studies necessary to begin the clinical evaluation and the analytical tools such as Trichoscopy, Phototrichogram, Dermatoscopy allowing an analysis of the targeted follicular units and an automated calculation of the data (number of hairs at each evaluation, relation with previous measurements). It also allows the analysis of the density and diameter of the hairs in different areas of the scalp, as well as access to their actual distribution in the follicular units. The trichoscopic evaluation is carried out in circular fields of vision of predefined size, as required for clinical trials or scientific research, since this function allows synchronizing symmetrical sites for the evaluation, regardless of the angle used to obtain the images of the studied sites.

[0131] Measurements of hair density, hair diameter and distribution in follicular units are also automatically integrated, allowing for secure marking and counting.

[0132] The Phototrichogram section of this tool, is widely recognized and prevalent in the clinical practice of trichology due to its high accuracy. We used this methodology to distinguish subclinical forms of female alopecia at early stages of the disease, to perform a differential diagnosis between androgenetic alopecia (AGA) and diffuse alopecia of chronic telogen effluvium (CTE) in order to evaluate the effectiveness of our serum. This program calculates the total number of hairs per square centimeter of skin, the quantities and percentage of telogen and vellus in the telogen phase. The study of the Phototrichogram also allows to determine the average rate of hair growth.

[0133] Values ​​are expressed as median ± SE; p<0.05 by t-test.

[0134] 2.4. Evaluation protocol

[0135] 1st day: Each participant in group A representing the serum intervention arm (serum from example 1) and each participant in group B representing the control arm (Placebo) are given dropper bottles of solution of the same galenic, without any possible identification, neither external nor difference opacity or texture, in sufficient quantity for 4 weeks of treatment as well as a unique application method. All participants also receive the same neutral shampoo to use throughout the clinical study, to ensure that the subjects' personal hygiene products do not interfere with the study results. These two precautions allowed us to standardize the intervention.

[0136] 1st to 30th day: the subjects apply 30 drops of solution every morning directly to the clean scalp, massaging lightly until completely absorbed. The solution is not rinsed until the following morning. The application method is demonstrated on the 1st day by applying the solution assigned to each subject by a specialist who is not informed of the group membership, therefore blind.

[0137] Visit 1: On the 30th day, the subjects are evaluated by a specialist, unaware of the subjects' group membership, in order to verify the correct application of the solution and to evaluate the results at four weeks on hair loss, hair density and hair diameter. The subjects are again given dropper bottles of solution in sufficient quantity for the following four weeks.

[0138] From the 31st to the 61st day, the subjects continue to apply their solution according to the same protocol.

[0139] Visit 2: On day 61, the subjects are evaluated by a specialist who is unaware of the subjects' group membership in order to verify the correct application of the solution and to evaluate the results at eight weeks on hair loss, hair density and hair diameter. The subjects are again given dropper bottles of solution in sufficient quantity for the following four weeks.

[0140] From the 62nd to the 92nd day, the subjects continue to apply their solution according to the same protocol.

[0141] Visit 3: On the 93rd day, the subjects are evaluated by a specialist who is unaware of the subjects' group membership, in order to establish the results at twelve weeks on hair loss, hair density and hair diameter.

[0142] 2.5. Observed results

[0143] The results are shown in Figures 1, 2, 3 and 4.

[0144] A significant reduction in hair loss was observed in the subjects in the intervention arm group A receiving 30 drops of the anti-hair loss serum daily (serum of example 1) compared to the control arm group B, quantified in the number of hairs lost during the reference period represented by 90 days. clinical study. The reduction in hair loss was gradual, measurements were taken at 1, 2 and 3 months of application of the serum with a respective percentage reduction of -16%, -35% and -40%.

[0145] The stimulation of regrowth could be demonstrated in the intervention arm by a clear increase in hair density in the subjects who used the serum of example 1. This increase in hair density was observed at 1, 2 and 3 months of application of the serum of example 1, with a respective percentage of +8%, +17% and +23%.

[0146] The average hair diameter also increased progressively in the intervention arm, measured in mm at 1, 2 and 3 months of application of the serum of example 1 to reach +6% at the end of the study compared to the initial measurements.

[0147] Participant satisfaction in the intervention arm was also significantly positive. Subjects using HFC serum reported, through closed-ended questionnaires, a clear improvement in their hair volume, less scalp irritation, better hair health, and a significant improvement in their perception of hair loss and their image, which they reported had a positive impact on their quality of life and socio-professional relationships.

[0148] The increase in hair density is considered one of the best parameters of any clinical study evaluating hair regrowth. As such, the Comparative Scoring Criteria, widely accepted as an evaluation tool, is based on the actual increase in hair density to categorize the effects of the different interventions. In our study, we took this tool into consideration for our quantitative measurements and to highlight the differences between the two groups. Thus, the intervention arm or group A receiving the serum of example 1 saw an increase in hair density measured in number of hairs / cm2 during the intermediate measurements at 30 and 60 days and those at the end at 90 days compared to the initial measurements.

[0149] The application of the serum of example 1 showed in 3 months a regrowth of >40 hairs / cm2 which is considered excellent regrowth. In comparison, good regrowth is estimated at 20-39.99 hairs / cm2, moderate regrowth at 10-19.99 hairs / cm2, poor regrowth at 5-9.99 hairs / cm2 and no regrowth or ineffectiveness of the product if <5 hairs / cm2. The improvement in the ranking of the participants in group A on the international scales also testifies to this effectiveness.

[0150] 2.5.1. Long-term assessment of the degree of hair loss, according to classifications international and age groups

[0151] [Tables4] Scales Group A Group B Ludwig I 3 0 Ludwig II 5 4 Ludwig III 2 6 Norwood-Hamilton II 4 0 Norwood-Hamilton III 3 3 Norwood-Hamilton IV 3 7

[0152] 2.5. 2. Safety assessment

[0153] Any topical treatment can result in side effects, expected or not, and of varying magnitude. We gave participants the opportunity to report any direct or related side effects, during the questionnaires given to them, in order to index them and compare them, if necessary, to other hair regrowth-stimulating products available on the market.

[0154] Based on the collected data, we found no notable side effects among the treated participants. The safety of application of the serum of Example 1 is rated 'A' according to the following criteria:

[0155] [Tables5 A No side effect or very mild transient side effect B Moderate and transient side effect C Allergic reaction, requiring discontinuation of treatment D Severe reaction requiring therapeutic action

[0156] 2.5. 3. Self-assessment results by questionnaires

[0157] [Tableauxô] Group A (n=20) Initial assessment Day 0 Visit 1 Day 30 Visit 2 Day 60 Visit 3 Day 90 Fall score 1 (100%) 1 (70%) 2 (30%) 2 (60%) 3 (40%) 3 (80%) 4 (20%) Texture score 1 (60%) 2 (40%) 1 (40%) 2 (30%) 3 (30%) 2 (30%) 3 (50%) 4 (20%) 3 (40%) 4 (20%) 5 (40%) Volume score 1 (70%) 2 (30%) 1 (50%) 2 (50%) 2 (60%) 3 (40%) 4 (80%) 5 (20%) 1 (50%) Itching score 2 (30%) 3 (2 0%) 1 (60%) 2 (4 0%) 1 (70%) 2 (30%) 1 (100%) Perceived severity of baldness score 4 (10%) 5 (9 0%) 3 (70%) 4 (30%) 2 (40%) 3 (60%) 1 (80%) 2 (20%) Quality of Life score 3 (70%) 4 (30%) 3 (80%) 4 (20%) 2 (40%) 3 (60%) 1 (60%) 2 (40%)

[0158] [Tables?] Group B (n=20) Initial assessment Day 0 Visit 1 Day 30 Visit 2 Day 60 Visit 3 Day 90 Hair loss score 1 (100%) 1 (90%) 2(10%) 1 (90%) 2 (10%) 1 (90%) 2 (10%) Texture score 1 (90%) 2(10%) 2 (30%) 3 (70%) 2 (20%) 3 (80%) 2 (20%) 3 (80%) Volume score 1 (80%) 2 (20%) 1 (80%) 2 (20%) 2 (100%) 2 (100%) Itching score 1 (50%) 2 (30%) 3 (20%) 1 (50%) 2 (40%) 3 (10%) 1 (60%) 2 (40%) 1 (60%) 2 (40%) Perceived severity score baldness 4 (10%) 5 (90%) 4 (30%) 5 (70%) 4 (30%) 5 (70%) 4 (30%) 5 (70%) Quality of Life Score 4 (20%) 5 (80%) 4 (20%) 5 (80%) 4 (30%) 5 (70%) 4 (30%) 5 (70%)

[0159] Caption:

[0160] *Observed hair loss rate: 1 = more than 100, 2 = 50-100, 3 = 30-50, 4 = 10-30, 5 = less than 10.

[0161] *Hair texture rating: 1 = coarse, brittle, dry hair, 2= dry, 3= normal, 4= soft, 5= very soft.

[0162] * Volume score evaluation: 1= very little volume, 2= little volume, 3= volume average, 4= good volume, 5= excellent volume.

[0163] *Itching assessment: 1= no itching, 2= slight itching, 3= moderate itching, 4= significant itching, 5= intense itching affecting daily life.

[0164] * Assessment of perceived degree of baldness: 1= no baldness 2= slight baldness, 3= moderate baldness, 4= noticeable baldness, 5= very visible baldness.

[0165] *Evaluation of negative impact of hair loss on Quality of Life: 1= no impact, 2= little impact, 3= significant impact, 4= disabling, 5= very disabling

[0166] At the end of our clinical study, the self-assessment results of the participants in the intervention arm group A, showed that: - 96% of treated participants would be willing to purchase the serum from Example 1 again. - 95% of treated participants found a significant improvement in the general condition of their hair. - 92% of treated participants felt that the serum in Example 1 helped to slow down their hair loss. - 90% of treated participants found their hair more resistant and less brittle. - 88% of treated participants felt their hair was thicker. - 85% of treated participants would recommend the serum from example 1 to a loved one.

[0167] 2.6. Conclusion

[0168] This clinical evaluation demonstrated the effectiveness of the serum of example 1 composed of a synergistic association of active ingredients transported in the environment close to the hair follicles thanks to an optimization of their penetration for this galenic form.

[0169] Data from this clinical study indicate that the Serum of Example 1 is effective in reducing hair loss, stimulating regrowth and improving hair density with notable user satisfaction. No side effects were observed and no participants were lost to follow-up or discontinued the protocol.

[0170] In accordance with the International Hair Science Association's Comparative Scoring Criteria, the clinical evaluation on the serum of Example 1 meets the five criteria established by the association: - effectiveness in hair regrowth, - the quality of the data collected, - safety and absence of side effects when applied, - the practicability of use, - inclusivity, the serum can be used regardless of gender, skin phototype and hair type.

[0171] These results support the effectiveness of the Serum of Example 1 and suggest that it can be recommended as an effective and safe solution to combat hair loss or to preserve hair capital.

Claims

Claims

1. Hair composition for skin and follicular penetration characterized in that it comprises: (A) from 45% to 95% by weight of exosomes derived from plant stem cells, (B) from 2% to 40% by weight of an extract of fruit of the palm tree of the genus Serenoa, (C) from 1% to 15% by weight of an extract of hairy root culture of Ocimum Basilicum, (D) from 0.05% to 10% by weight of Niacinamide, (E) from 0.05% to 10% by weight of essential oil of Rosemary, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

2. Composition according to claim 1, characterized in that it comprises: (A) from 60% to 94% by weight, in particular from 75% to 93% by weight, more particularly from 85% to 92% by weight of exosomes derived from plant stem cells, (B) from 3% to 30% by weight, in particular from 4% to 20% by weight, more particularly from 5% to 10% by weight of an extract of fruit of the palm tree of the genus Serenoa, (C) from 1% to 10% by weight, in particular from 1.5% to 7% by weight, more particularly from 1.5% to 4% by weight of an extract of hairy root culture of Ocimum Basilicum, (D) from 0.1% to 8% by weight, in particular from 0.2% to 5% by weight, more particularly from 0.3% to 1% by weight of Niacinamide, (E) from 0.08% to 8% by weight, in particular from 0.1% to 5% by weight, more particularly from 0.15% to 1% by weight of essential oil of Rosemary, the percentages by weight being expressed relative to the sum of the weights of (A), (B), (C), (D) and (E).

3. Composition according to claim 1 or 2, characterized in that the exosomes derived from plant stem cells are chosen from exosomes derived from stem cells of flowering plants of the Curcubitaceae family, in particular of the genus Lagenaria, such as Lagenaria siceraria, of the genus Sicyos, such as Sicyos angulatus, or of the genus Benincasa, such as Benincasa hispida, or of the family Rosacea e, notably of the genus Malus, such as Malus domestica, of the genus Prunus, such as Prunus armeniaca or Prunus dulcis, or of the genus Rosa, such as Rosa gallica or Rosa canina.

4. Composition according to any one of claims 1 to 3, characterized in that it further comprises a vitamin, in particular chosen from group B such as: B1, B2, B5, B6, B7, B8, B12, a vitamin C, a vitamin E, or one of their mixtures.

5. Composition according to any one of claims 1 to 4, characterized in that it further comprises a humectant or moisturizing agent, in particular chosen from: glycerin, aloe vera, soybean germ extract, glycosaminoglycan, retinyl palmitate, ferric sulfate or one of their mixtures.

6. Composition according to any one of claims 1 to 5, characterized in that it further comprises a conditioner, in particular chosen from: hydrolyzed keratin, coconut extract, sunflower seed oil, or a vasodilating agent, such as Mentha piperita essential oil.

7. Composition according to any one of claims 1 to 6, characterized in that it further comprises a preservative agent, an antiseptic agent, an antimicrobial agent, an antioxidant agent and / or a pH regulator.

8. Composition according to any one of claims 1 to 7, characterized in that it further comprises an emulsifying agent, a surfactant, a gelling agent, a viscosity control agent, a swelling agent, a coloring agent and / or a perfuming agent.

9. Composition according to any one of claims 1 to 8, characterized in that it is in the form of a lotion, a serum, a shampoo, a conditioner, a dry shampoo, a gel, a wax, a lacquer, a spray, a mousse, a paste, a cream, a powder, an oil, a mask, a treatment, a tonic, said composition optionally further comprising an aqueous, alcoholic or hydroalcoholic solvent.

10. Composition according to any one of claims 1 to 9, for use in preventing or reducing hair loss.

11. Composition for use according to claim 10, characterized in that the composition is applied to the scalp, and the application is optionally followed by a massage or friction of the scalp.

12. Composition for its use according to claim 11, characterized in that the composition is further rinsed.

13. Cosmetic use of the composition according to any one of claims 1 to 9 for increasing hair density, or increasing hair diameter.

14. A cosmetic hair treatment method comprising applying the composition according to any one of claims 1 to 9 to the scalp, optionally followed by massaging or rubbing the scalp.

15. The method of claim 14 further comprising a step of rinsing the composition.

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