Hydrogen peroxide damage inhibitor

Abstract

To discover, from among highly safe plant-derived components, one having hydrogen peroxide damage inhibitory action, and to provide a hydrogen peroxide damage inhibitor including the discovered one as an active ingredient.SOLUTION: A hydrogen peroxide damage inhibitor herein includes, as an active ingredient, at least one plant-derived material selected from the group consisting of Isodon japonicus extract, Sasa veitchii RERD. extract, Eucalyptus globulus L. extract, Melissa officinalis L. extract, Zingiber officinale extract, Sanguisorba officinalis L. extract, Morinda citrifolia fruit juice, Uncaria gambir extract, Hydrangea serrata extract, Lonicera japonica Thunberg. extract, Averrhoa carambola extract, Eriobotrya japonica Lindley extract and Paeonia suffruticosa extract.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an agent for suppressing damage caused by hydrogen peroxide, and more particularly to an agent for suppressing damage caused by hydrogen peroxide, which contains a plant-derived component as an active ingredient. [Background technology]

[0002] Hydrogen peroxide is a type of reactive oxygen species, which is essential for the bactericidal mechanism of phagocytes and plays an important role in removing viruses and cancer cells. However, excessive production of reactive oxygen species attacks the biological molecules that make up membranes and tissues in the body, inducing various diseases, and is therefore considered to be oxidative stress. Factors that cause oxidative stress include physiological factors such as aging, as well as environmental factors such as ultraviolet radiation, smoking, and chemicals. One tissue that is susceptible to such oxidative stress is the skin, including the scalp.

[0003] In particular, factors that cause oxidative stress on the scalp include hair dyes and perm agents. In recent years, with the diversification of hairstyles, the number of occasions to treat hair with hair dyes and perm agents has increased, and two-component oxidative hair dyes and perm agents that use hydrogen peroxide are also widely used. When these oxidative hair dyes and perm agents are used, the scalp is exposed to hydrogen peroxide and damaged.

[0004] Here, in the scalp, a relationship between oxidative stress and alopecia has been reported (for reviews, see Non-Patent Documents 1 and 2). Hair grows and falls out repeatedly according to a cyclical hair cycle consisting of an anagen phase, a regressive phase, and a telogen phase. In this hair cycle, the stage in which new hair follicles are formed from the telogen phase to the anagen phase is considered to be the most important for hair growth. In this stage, dermal papilla cells play an important role in the proliferation and differentiation of hair follicle epithelial cells. When dermal papilla cells are damaged by oxidative stress, the cell cycle stops and apoptosis occurs, disrupting the hair cycle, which is considered to be the cause of hair loss.

[0005] Furthermore, oxidative stress can induce inflammation and pigmentation in the skin, such as the scalp, and can also produce lipid peroxides that oxidize lipids and damage cells, resulting in skin aging such as the formation of wrinkles and reduced elasticity.

[0006] Therefore, if the cell damage caused by hydrogen peroxide can be suppressed and cell homeostasis can be improved, it is believed that the above-mentioned group of diseases caused by oxidative stress due to hydrogen peroxide can be prevented and improved. For example, Yakushima Hydrangea extract (Patent Document 1), Long Pepper extract (Patent Document 2), etc. are known to have such an effect of suppressing damage caused by hydrogen peroxide. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] JP 2011-184392 A [Patent Document 2] JP 2012-232920 A [Non-patent literature]

[0008] [Non-Patent Document 1] Int. J. Trichology,2009,vol.1,issue 1,pp.6-14 [Non-Patent Document 2] Int. J. Cosmetic Sci.,2015, vol.37, issue S2, pp.25-30 Summary of the Invention [Problem to be solved by the invention]

[0009] The present invention aims to find a highly safe plant-derived ingredient that has the effect of inhibiting hydrogen peroxide damage, and to provide an agent for inhibiting hydrogen peroxide damage that contains the ingredient as an active ingredient. [Means for solving the problem]

[0010] In order to solve the above problems, the hydrogen peroxide damage inhibitor of the present invention is an extract of Enmeisou and an extract of Kumazasa. 、 Lemon balm extract, and Noni juice from The present invention is characterized in that the active ingredient is one or more plant-derived substances selected from the group consisting of: Effect of the Invention

[0011] According to the present invention, the extract of Enmeisou and the extract of Kumazasa 、 Lemon balm extract, and Noni juice from By using one or more plant-derived substances selected from the group consisting of the above as an active ingredient, it is possible to provide an agent for suppressing damage caused by hydrogen peroxide that is excellent in function and effectiveness and highly safe. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] Hereinafter, an embodiment of the present invention will be described. The hydrogen peroxide damage inhibitor of the present embodiment is an extract of Enmeisou, an extract of Kumazasa 、 Lemon balm extract, and Noni juice from The present invention relates to a composition comprising, as an active ingredient, one or more plant-derived substances selected from the group consisting of:

[0013] [Plant extract] In this embodiment, Enmeisou extract, Kumazasa extract and Lemon Balm Extract of The raw materials used for the production of this product are Enmeiso (scientific name: Isodon japonicus or I. trichocarpus), Kumazasa (scientific name: Sasa veitchii RERD.), and Lemon balm (scientific name: Melissa officinalis L.) is .

[0014] Enmeisou (Enmeisou) is another name for Isodon japonicus or Isodon trichocarpus, which is a perennial herb of the family Lamiaceae, genus Mentha. They are distributed from Hokkaido to Kyushu and can be easily obtained from these areas. There is no particular restriction on the parts that can be used as the raw material for extraction, and they can be appropriately selected depending on the purpose, but examples include above-ground parts such as leaves, branches, stems, flowers, buds, seeds, etc.; roots; or mixtures thereof, and among these, above-ground parts are preferred.

[0015] Kumazasa (Sasa veitchii RERD.) is a plant belonging to the genus Sasa in the family Poaceae, and grows naturally in the Kinki, Chugoku, and Kyushu regions, and can be easily obtained from these regions. There are no particular limitations on the parts that can be used as the raw material for extraction, and they can be appropriately selected depending on the purpose, but examples include leaves, stems, rhizomes, and mixtures of these, and among these, leaves are preferred.

[0016] Lemon balm (Melissa officinalis L.), also known as Melissa, is a perennial herb of the Lamiaceae family, and is distributed throughout Europe, Central Asia, North America, etc., and can be easily obtained from these regions. There is no particular limitation on the parts that can be used as the raw material for extraction, and they can be appropriately selected depending on the purpose, and examples of the parts that can be used include above-ground parts such as leaves, stems, and flowers; roots; or mixtures of these parts, and among these, leaves are preferred.

[0017] The extract from the above-mentioned plant can be obtained by drying the extraction raw material, pulverizing it using a crusher, or by using a common dryer, and then subjecting it to extraction using an extraction solvent. Drying can be performed in the sun or by using a commonly used dryer. In addition, the raw material can be used after pretreatment such as degreasing with a non-polar solvent such as hexane. By performing pretreatment such as degreasing, extraction treatment with a polar solvent can be performed efficiently.

[0018] As the extraction solvent, it is preferable to use a polar solvent, such as water or a hydrophilic organic solvent, which is preferably used alone or in combination of two or more at room temperature or at a temperature below the boiling point of the solvent.

[0019] Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral water, hot spring water, spring water, fresh water, etc., as well as water that has been subjected to various treatments. Treatments that can be applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, etc. Therefore, water that can be used as an extraction solvent in this embodiment also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered physiological saline, etc.

[0020] Examples of hydrophilic organic solvents that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms, such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; polyhydric alcohols having 2 to 5 carbon atoms, such as 1,3-butylene glycol, propylene glycol, and glycerin; and lower aliphatic ketones having 3 to 4 carbon atoms, such as acetone and methyl ethyl ketone.

[0021] When a mixture of two or more polar solvents is used as an extraction solvent, the mixture ratio is arbitrary and can be adjusted appropriately. For example, when a mixture of water and a hydrophilic organic solvent is used as an extraction solvent, the mixture ratio can be arbitrarily mixed and used, that is, between more than 0:100 and less than 100:0 (volume ratio, hereinafter the same), and can be adjusted appropriately. For example, when a mixture of water and a lower aliphatic alcohol is used as an extraction solvent, the ratio (volume %) of the lower aliphatic alcohol in the mixture can be 10% or more, or even 30% or more, or the ratio (volume %) of the lower aliphatic alcohol in the mixture can be 90% or less, or even 80% or less. When a mixture of water and a polyhydric alcohol is used, the ratio (volume %) of the polyhydric alcohol in the mixture can be 20% or more, or 90% or less, and when a mixture of water and a lower aliphatic ketone is used, the ratio (volume %) of the lower aliphatic ketone in the mixture can be 10% or more, or 80% or less.

[0022] The extraction process is not particularly limited as long as the soluble components contained in the extraction raw material can be dissolved in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent of 5 to 15 times the amount (mass ratio) of the extraction raw material, and the soluble components are extracted at room temperature or under reflux heating, and then the extraction residue is removed by filtration to obtain an extract. The solvent is distilled off from the obtained extract to obtain a paste-like concentrate, and the concentrate is further dried to obtain a dry product. In addition, the "extract" in this embodiment includes an extract obtained using the above-mentioned plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or any of these crude or purified products.

[0023] [Noni juice] In this embodiment, the raw material for obtaining noni juice is noni (scientific name: Morinda citrifolia).

[0024] Noni (Morinda citrifolia), known as Yaeyama Aoki in Japan, is a plant of the Rubiaceae family, and is distributed in areas such as Okinawa to the Ogasawara Islands, Taiwan to China, and the coastal areas of India, and is easily available from these areas. The part used in this embodiment is the fruit. Noni juice can be obtained by squeezing the juice from the noni fruit using a juicer or similar device.

[0025] The obtained noni juice may be used as it is, or may be concentrated or dried to be used as a concentrate or a dried product. When dried, a freeze-dried product is preferred. In addition, the obtained noni fruit juice may be further subjected to an extraction process and used as an extract. The extraction solvent and extraction process described above for the plant extract can be suitably applied to the extraction of noni fruit juice.

[0026] [Hydrogen peroxide damage inhibitor] The thus obtained Enmeiso extract and Kumazasa extract 、 Lemon balm extract, and Noni juice teeth Both of these have excellent hydrogen peroxide damage suppressing properties and can therefore be used as active ingredients in hydrogen peroxide damage suppressants.

[0027] In other words, to produce a hydrogen peroxide damage inhibitor, Enmeiso extract, Kumazasa extract 、 Lemon balm extract, and Noni juice from One or more plant-derived products selected from the group consisting of can be used. In the following, Enmeisou extract and Kumazasa extract are 、 Lemon balm extract, and Noni juice of These may be collectively referred to as "the plant-derived product of this embodiment" or "the above-mentioned plant-derived product".

[0028] The hydrogen peroxide damage inhibitor of the present embodiment can be used in a wide range of applications, such as medicines, quasi-drugs, cosmetics, and oral compositions.

[0029] The hydrogen peroxide damage inhibitor of the present embodiment may consist solely of the above-mentioned plant-derived material, or may be a formulation containing the above-mentioned plant-derived material.

[0030] The hydrogen peroxide damage inhibitor of the present embodiment can be formulated into any dosage form, such as powder, granules, tablets, liquid, etc., using a pharma- ceutically acceptable carrier such as dextrin, cyclodextrin, or any other auxiliary agent according to a conventional method. In this case, examples of auxiliary agents that can be used include excipients, binders, disintegrants, lubricants, stabilizers, flavorings, and odorants. The hydrogen peroxide damage inhibitor can be used by blending with other compositions (e.g., scalp and hair cosmetics, skin cosmetics, oral compositions, etc.), and can also be used as ointments, external liquids, patches, etc.

[0031] When the hydrogen peroxide damage suppressant of the present embodiment is formulated, the content of the plant-derived material is not particularly limited and can be appropriately set depending on the purpose.

[0032] In addition, the hydrogen peroxide damage inhibitor of the present embodiment can be used as an active ingredient by blending other natural extracts, etc. having an inhibitory effect on hydrogen peroxide damage with the above-mentioned plant-derived product, as necessary.

[0033] The method of administering the hydrogen peroxide damage inhibitor of this embodiment to a patient may be percutaneous administration, oral administration, etc., and a method suitable for prevention, treatment, etc. may be appropriately selected depending on the type of disease. The dosage of the hydrogen peroxide damage inhibitor of this embodiment may also be appropriately increased or decreased depending on the type and severity of the disease, individual differences between patients, administration method, administration period, etc.

[0034] The hydrogen peroxide damage inhibitor of the present embodiment can suppress cell damage caused by hydrogen peroxide due to the action of the plant-derived active ingredient. This can normalize the hair cycle in the skin such as the scalp, thereby preventing, treating or improving alopecia. The hydrogen peroxide damage inhibitor of the present embodiment is particularly suitable for application to the scalp, particularly hair papilla cells, and can be said to be suitable for hair growth applications based on the hydrogen peroxide damage suppressing action. In addition, the hydrogen peroxide damage inhibitor of the present embodiment, through its hydrogen peroxide damage inhibitory effect, can suppress hydrogen peroxide damage that can occur to the scalp when hair is treated with scalp and hair cosmetics containing hydrogen peroxide (e.g., oxidative hair dyes, perm agents, etc.). However, the hydrogen peroxide damage inhibitor of the present embodiment can be used in all applications other than those mentioned above in which it is meaningful to exert an inhibitory effect against hydrogen peroxide damage.

[0035] For example, the hydrogen peroxide damage inhibitor of the present embodiment can prevent, treat, or improve skin aging, skin inflammation, skin pigmentation, and the like in the skin, such as the scalp, through its hydrogen peroxide damage inhibitory effect.

[0036] The hydrogen peroxide damage inhibitor of the present embodiment has an excellent hydrogen peroxide damage inhibitory effect, and is therefore suitable for incorporation into, for example, skin external preparations and oral compositions. In this case, the plant-derived material may be incorporated as is, or a hydrogen peroxide damage inhibitor formulated from the plant-derived material may be incorporated.

[0037] Here, the topical skin preparation is not limited to a particular category, and includes a wide range of products, including scalp and hair cosmetics described below, skin cosmetics, quasi-drugs for transdermal use, and pharmaceuticals.

[0038] In addition, the oral composition refers to a composition that is unlikely to cause harm to human health and is taken orally or by administration to the digestive tract in normal social life, and is not limited to administrative classifications such as food, medicine, and quasi-drug. Therefore, the "oral composition" in this embodiment broadly includes general foods, health foods, health functional foods (specified health foods, nutritional functional foods, functional food), quasi-drugs, medicines, etc. that are taken orally. The oral composition according to this embodiment is preferably an oral composition that can display the favorable effects of the plant-derived substances on the oral composition or its packaging, and is particularly preferably a health functional food (specified health foods, functional food, nutritional functional food), quasi-drug, and medicine.

[0039] [Scalp and hair cosmetics] The plant-derived material has an excellent hydrogen peroxide damage suppressing effect, and is particularly suitable for application to the scalp, and is therefore suitable for incorporation into scalp and hair cosmetics. In this case, the plant-derived material may be incorporated as is, or a hydrogen peroxide damage suppressing agent formulated from the plant-derived material may be incorporated. By incorporating the plant-derived material or the hydrogen peroxide damage suppressing agent, a scalp and hair cosmetic suitable for hydrogen peroxide damage suppression applications can be obtained.

[0040] The type of scalp and hair cosmetic that can contain the plant-derived product or the formulated hydrogen peroxide damage inhibitor is not particularly limited, and examples include hair tonic, hair cream, hair liquid, shampoo, pomade, rinse, hair treatment, hair spray, perm agent, hair dye, etc. Furthermore, the formulation may be in the form of liquid, cream, paste, foam, mist, powder, etc.

[0041] When the above-mentioned plant-derived product or formulated hydrogen peroxide damage inhibitor is incorporated into a scalp and hair cosmetic, the amount of the product can be adjusted as appropriate depending on the type of scalp and hair cosmetic. A suitable ratio is about 0.0001 to 10 mass % calculated as the solid content of the plant-derived product, and a particularly suitable ratio is about 0.001 to 1 mass %.

[0042] As long as the hydrogen peroxide damage-inhibiting effect of the plant-derived substance is not hindered, the scalp and hair cosmetic of this embodiment can be used in combination with a main ingredient, auxiliary agent, or other ingredient used in the manufacture of a normal scalp and hair cosmetic, such as an astringent, a germicide or antibacterial agent, a whitening agent, an ultraviolet absorber, a moisturizer, a cell activator, an anti-inflammatory or anti-allergic agent, an antioxidant or active oxygen remover, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, etc. By using such a combination, a more general product can be obtained, and the synergistic effect with the other active ingredient used in combination can bring about a superior effect than would normally be expected.

[0043] The scalp and hair cosmetic composition of this embodiment can be used for preventing, treating or improving alopecia (hair growth applications) through its effect of inhibiting hydrogen peroxide damage; inhibiting hydrogen peroxide damage that may be caused by scalp and hair cosmetics containing hydrogen peroxide; and preventing, treating or improving skin aging, skin inflammation, skin pigmentation, etc. in the skin, such as the scalp.

[0044] The hydrogen peroxide damage inhibitor of the present embodiment has an excellent hydrogen peroxide damage inhibitory effect, and therefore can be suitably used as a reagent for research into the mechanism of action thereof.

[0045] Although the hydrogen peroxide damage inhibitor of this embodiment is suitable for use in humans, it can also be used in animals other than humans (e.g., mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.) as long as the respective functional effects are achieved. EXAMPLES

[0046] The present invention will be specifically described below with reference to Production Examples and Test Examples, but the present invention is not limited to the following examples in any way.

[0047] [Production Example 1] Production of Enmeisou extract 1500mL of 50% ethanol by volume was added to 100g of dried aerial parts of the Japanese knotweed, and the mixture was extracted by heating at 80-90℃ for 2 hours in a reflux extractor, followed by hot filtration. The resulting extract was dried to obtain the Japanese knotweed extract (15g, sample 1).

[0048] [Production Example 2] Production of Kumazasa extract 1500 mL of 50% ethanol by volume was added to 100 g of dried Kumazasa leaves, and extraction was performed by heating at 80-90°C for 2 hours in a reflux extractor, followed by hot filtration. The resulting extract was dried to obtain Kumazasa extract (5 g, sample 2).

[0049] [Production example 3 〕Production of Lemon Balm Extract 1500 mL of 50% by volume 1,3-butylene glycol was added to 100 g of dried lemon balm leaves, and the mixture was heated and extracted at 80-90°C for 2 hours in a reflux extractor and filtered while hot. The resulting extract was dried to obtain the lemon balm extract (20 g, sample 3 ) was obtained.

[0050] [Production example 4 〕Production of Noni juice Butylene glycol and purified water were added to 100 g of noni juice to adjust the content. The obtained liquid was dried to obtain the solid content of noni juice (5 g, sample 4 ) was obtained.

[0051] [Test Example 1] Test of hydrogen peroxide damage inhibition effect on human hair papilla cells Normal human hair papilla cells (purchased from Takara Bio) were cultured in a hair papilla cell growth medium (purchased from Takara Bio), and then harvested by trypsinization. The harvested cells were collected at a concentration of 1.5 × 10 5 After diluting with hair papilla cell proliferation medium to a concentration of 100 cells / mL, the cells were seeded at 100 μL per well on a 96-well plate and cultured overnight.

[0052] After the culture was completed, the medium was removed and the test samples (samples 1 to 4 100 μL of dermal papilla cell growth medium containing 0.1 mmol / L hydrogen peroxide (final concentration, see Table 1 below) was added to each well and cultured for 24 hours. As a control, dermal papilla cell growth medium without the sample was used and cultured in the same manner. After culture was completed, the medium was removed and the wells were washed with 200 μL of PBS(-). After washing, 100 μL of hydrogen peroxide (0.2 mmol / L) dissolved in Hanks' buffer solution or Hanks' buffer solution alone was added to each well and cultured for 2 hours.

[0053] After incubation, 100 μL of neutral red dissolved in PBS(-) at a final concentration of 0.05 mg / mL was added to each well and incubated for 2 hours. After incubation, the neutral red solution was removed and 100 μL of an ethanol-acetic acid solution (ethanol:acetic acid:water = 50:1:49) was added to each well to extract the pigment. After extraction, the absorbance was measured at a wavelength of 540 nm. The hydrogen peroxide damage inhibition rate (%) was calculated from the measurement results using the following formula.

[0054] Hydrogen peroxide damage inhibition rate (%) = (AB) / (CB) x 100 A: Absorbance at 540 nm after hydrogen peroxide treatment and addition of test sample B: Absorbance at 540 nm after hydrogen peroxide treatment and without sample C: Absorbance at 540 nm without hydrogen peroxide treatment or sample addition The results are shown in Table 1.

[0055] [Table 1]

[0056] As shown in Table 1, Enmeiso extract (sample 1) and Kumazasa extract (sample 2) 、 Lemon balm extract (sample 3 ), and Noni juice (sample 4 ) teeth Both were found to have excellent inhibitory effects against hydrogen peroxide damage.

[0057] [Formulation example 1] A hair tonic having the following composition was prepared in a conventional manner. Enmeisou extract (Production Example 1) 0.4g Tocopherol acetate (appropriate amount) Cephalatin 0.002g Isopropyl methylphenol 0.1g Sodium hyaluronate 0.15g Glycerin 15.0g Ethanol 15.0g Fragrance (appropriate amount) Chelating agent (sodium edetate) appropriate amount Preservative (hinokitiol) appropriate amount Solubilizer (polyoxyethylene cetyl ether) appropriate amount Purified water Remainder (total amount is 100g)

[0058] [Formulation example 2 〕 A shampoo having the following composition was prepared by a conventional method. Kumazasa extract (Production Example 2) 0.5g Sodium coconut oil fatty acid methyl taurate 10.0g Coconut oil fatty acid amidopropyl betaine 10.0g Sodium polyoxyethylene alkyl ether sulfate 20.0g Coconut oil fatty acid diethanolamide 4.0g Propylene glycol 2.0g Fragrance (appropriate amount) Purified water Remainder (total amount is 100g)

[0059] [Formulation example 3 〕 A shampoo having the following composition was prepared by a conventional method. Noni juice (production example) 4 ) 0.5g Star Fruit Extract 0.5g Sodium coconut oil fatty acid methyl taurate 10.0g Coconut oil fatty acid amidopropyl betaine 10.0g Sodium polyoxyethylene alkyl ether sulfate 20.0g Coconut oil fatty acid diethanolamide 4.0g Propylene glycol 2.0g Fragrance (appropriate amount) Purified water Remainder (total amount is 100g)

[0060] [Formulation example 4 〕 A rinse having the following composition was prepared by a conventional method. Lemon balm extract (production example)3 ) 0.2g Stearyltrimethylammonium chloride 1.5g Polyoxyethylene cetyl ether 1.0g Cetyl alcohol 2.0g Octyldodecanol 1.0g Cationic cellulose 0.5g Propylene glycol 5.0g Fragrance 3.0g Purified water Remainder (total amount is 100g)

[0061] [Formulation example 5 〕 The first and second parts having the following composition were produced by a conventional method and mixed in a mass ratio of 1:1 to prepare an oxidation hair dye. <First agent> Enmeisou extract (Production Example 1) 5.0g Paraphenylenediamine 1.0g Cetanol 5.0g Isopropyl myristate 1.0g 2.0g beeswax Polyoxyethylene oleyl alcohol 2.0g Sodium lauryl sulfate 1.0g Strong ammonia water 7.0g Resorcinol 0.3g Meta-aminophenol 0.5g Ascorbic acid 0.5g Fragrance (appropriate amount) Purified water Remainder (total amount is 100g) <Second agent> A 5.8% by weight aqueous solution of hydrogen peroxide adjusted to pH 3.0 with phosphoric acid

[0062] [Formulation example 6 〕 The first and second parts having the following composition were produced by a conventional method and mixed in a mass ratio of 1:1 to prepare an oxidation hair dye. <First agent> Kumazasa extract (Production Example 2) 5.0g Paraphenylenediamine 1.0g Cetanol 5.0g Isopropyl myristate 1.0g 2.0g beeswax Polyoxyethylene oleyl alcohol 2.0g Sodium lauryl sulfate 1.0g Strong ammonia water 7.0g Resorcinol 0.3g Meta-aminophenol 0.5g Ascorbic acid 0.5g Fragrance (appropriate amount) Purified water Remainder (total amount is 100g) <Second agent> A 5.8% by weight aqueous solution of hydrogen peroxide adjusted to pH 3.0 with phosphoric acid

[0063] [Formulation example 7 〕 First and second agents having the following composition were prepared by a conventional method, and a perm agent comprising the first and second agents was obtained. <First agent> Lemon balm extract (production example) 3 ) 0.5g Ginger Extract 0.5g Ammonium thioglycolate solution (50% as thioglycolic acid) 8.0g Ammonia water (25%) adjusted to pH 9, appropriate amount Polydimethylmethylene piperidinium chloride 0.4g Emulsifier 3.0g Purified water Remainder (total amount is 100g) <Second agent> Hydrogen peroxide (35%) - 6.6g Sodium pyrophosphate 0.2g Phosphoric acid (adjusted to pH 3.0) Lauryl dimethylaminoacetate betaine 1.0g Purified water Remainder (total amount is 100g)

Claims

[Claim 1] A hydrogen peroxide damage inhibitor characterized by comprising, as an active ingredient, one or more plant-derived substances selected from the group consisting of Enmeiso extract, lemon balm extract, Kumazasa extract and Noni juice.

Images