Topical skin preparation

Abstract

To provide a topical skin preparation made from natural products with superior biological safety, incorporating active ingredients with an effect of sustainedly enhancing moisture sensation, transparency, and gloss, an effect of repairing opened pores, an effect of activating oxidized ascorbic acid reductase in vivo, and an effect of suppressing the decline of skin firmness and preventing or improving wrinkles.SOLUTION: The present invention provides a topical skin preparation comprising an extract from one or more plants selected from Citrus aurantium and Phellodendron amurense Ruprecht as an active ingredient, where the used part of Citrus aurantium includes its peel.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an external skin preparation containing, as an active ingredient, an extract of one or two plants belonging to the Rutaceae family. [Background technology]

[0002] Skin aging or disorders is a phenomenon that occurs due to a complex intertwining of internal factors such as inactivation of cell proliferation and differentiation, decreased hormone secretion, and quantitative decrease in extracellular matrix components with aging, and external factors such as cell and tissue damage caused by active oxygen induced by sunlight (ultraviolet rays), or inflammation. Examples of skin aging or disorders include wrinkles, sagging, loss of elasticity, dryness, decreased barrier function, pigmentation, dullness, etc., and wrinkle improvement agents, moisturizers, age spot prevention agents, etc. have been proposed according to each skin condition.

[0003] Conventionally, antioxidants such as vitamins A and E and moisturizers (e.g., amino acids, organic acids, hyaluronic acid or its salts, chondroitin, heparinoids, etc.) have been proposed as ingredients for preventing or improving skin aging or disorders, but there are problems with stability, efficacy, and safety when blended into skin external preparations. In addition, the use of ingredients derived from natural products such as plants has also been proposed, but there are problems with efficacy. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 07-118132 [Patent Document 2] Japanese Patent Publication No. 08-295621 [Patent Document 3] Patent Publication No. 2000-096050 [Patent Document 4] JP2001-114634A DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0005] In view of the problems of the prior art, the present inventors have conducted extensive research to find new, highly effective plant-derived components, and as a result, have found that extracts from plants belonging to the Rutaceae family have the effect of continuously improving the moisturizing, translucency, and luster of the skin, the effect of activating an enzyme that reduces "oxidized ascorbic acid" produced by oxidation in the body, the effect of activating epidermal cells, the effect of activating fibroblasts, and the effect of promoting collagen synthesis.

[0006] The use of Rutaceae plants in topical skin preparations has been known in the past, for example, from Patent Documents 1 to 4. However, it was not known that these plants have the effects of sustainably improving the moisturizing, transparency, and luster of the skin, activating the enzyme that reduces oxidized ascorbic acid, activating epidermal cells, activating fibroblasts, and promoting collagen synthesis. [Means for solving the problem]

[0007] This is an external skin preparation for improving the skin's moisture, clarity and luster, containing as active ingredients an extract of one or two plants, namely, Citrus aurantium and Phellodendron amurense Ruprecht, which belong to the Rutaceae family. The present invention also relates to an activator of oxidized ascorbate reductase, comprising as an active ingredient an extract from one or two plants belonging to the Rutaceae family, namely, bitter orange and Phellodendron amplexicaule. The present invention also relates to an external skin preparation for inhibiting loss of skin elasticity or preventing and improving wrinkles, which contains as active ingredients an extract of one or two plants of either Bitter Orange or Phellodendron Amurense, which belong to the Rutaceae family. The present invention also relates to an external skin preparation for improving pores, which contains as active ingredients an extract of one or two plants belonging to the Rutaceae family, namely, bitter orange and Phellodendron amplexicaule. The present invention also relates to an external skin preparation containing as active ingredients an extract of Citrus Aurantium oryzae and an extract of Phellodendron Amurense, which belong to the Rutaceae family. Effect of the Invention

[0008] According to the present invention, by using an extract of one or two plants selected from the group consisting of an extract of bitter orange and an extract of Phellodendron amplexicaule as active ingredients, it is possible to provide an external skin preparation having the effect of continuously improving moisturization, transparency, and luster.

[0009] Furthermore, according to the present invention, by using an extract of one or two of the following plants as active ingredients, an extract of Bitter Orange and an extract of Phellodendron Amurense, it is possible to provide an external skin preparation that activates an enzyme that reduces oxidized ascorbic acid, converting it back to reduced ascorbic acid that exerts antioxidant properties, thereby suppressing the effects of active oxygen and the like on the skin and enhancing the effects of ascorbic acid (including derivatives) applied to the skin through external application.

[0010] Furthermore, according to the present invention, by using an extract of one or two of the following plants as active ingredients, an extract of Bitter Orange and an extract of Phellodendron Amurense can be provided, which activates dermal cells, inhibits loss of skin elasticity, and prevents and improves wrinkles.

[0011] Furthermore, according to the present invention, by using an extract of bitter orange as an active ingredient, it is possible to provide an external skin preparation that promotes the energy metabolism of epidermal cells, thereby regulating skin turnover and repairing open pores. [Brief description of the drawings]

[0012] [Figure 1] FIG. 1 is a graph showing the NF-κB activation inhibitory effect of the extract according to the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] The Rutaceae plant that can be used in the present invention is Citrus aurantium L. or Phellodendron amurense Ruprecht.

[0014] Examples of parts to be used include the fruit, peel, flesh, seeds, flowers, leaves, stems, etc. in the case of bitter orange, and the bark, etc. in the case of Phellodendron amplexicaule.

[0015] The preparation of the extract is carried out by first washing the part to be used for extraction with water if necessary to remove foreign matter, leaving it as it is or drying it, and then cutting or crushing it as necessary, and contacting it with an extraction solvent to carry out extraction. The extraction can be carried out by contacting it with an extraction solvent according to a conventional method such as the immersion method, but it is also possible to use a supercritical extraction method other than the immersion method.

[0016] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination of two or more.

[0017] Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoint of the effectiveness of the obtained extract, furthermore, skin irritation, and also from the viewpoint of wide applicability to skin external preparations (cosmetics, quasi-drugs, external medicines, etc.).Preferred examples of the hydrophilic solvent include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol, glycerin) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin), etc.

[0018] When a mixed solvent is used, the mixing ratio, for example, of water and a lower alcohol or a polyhydric alcohol, is preferably 1:5 to 25:1 in terms of volume ratio (the same applies below).

[0019] In preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkalinity regulator such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent to adjust the pH to a desired level.

[0020] The extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, when water alone or a mixture of water and a lower alcohol or a polyhydric alcohol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), and more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0021] In order to remove impurities and improve stability, the extract of the present invention may be subjected to one or a combination of two or more of activated carbon treatment, ion exchange resin treatment, synthetic adsorbent treatment, silica gel, and recrystallization treatment to remove impurities other than the active ingredients.

[0022] The extract prepared as described above may be used as an ingredient in a skin external preparation as it is after adjusting the pH to 3 to 8, or may be used at a desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0023] Examples of topical skin preparations (cosmetics, quasi-drugs, topical medicines) containing the extract of the present invention include, but are not limited to, milky lotions, creams, lotions, essences, packs, lipstick, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, soaps and other cleansing cosmetics, shampoos, conditioners, hair cosmetics such as hair growth products, bath additives, and the like.

[0024] When the extract according to the present invention is incorporated into an external skin preparation, the amount of the extract is generally in the range of 0.00001 to 5.0% by weight in terms of the solid content of each extract.

[0025] When the extract according to the present invention is incorporated into topical skin preparations (such as cosmetics, quasi-drugs, and topical pharmaceuticals), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic and natural), moisturizers, thickeners, emulsifiers or emulsification aids, preservatives and germicides, powder ingredients, UV absorbers, antioxidants, sequestering agents, pigments, fragrances, anti-wrinkle agents, pigmentation prevention agents, and other physiologically active ingredients, can be appropriately incorporated as necessary.

[0026] Examples of oily components include plant-derived oils such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, lemon peel oil, camphor bark oil, mandarin orange oil, cardamom oil, and orange oil; vitamin A oil; animal-derived oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, wax, and wax. Examples of suitable glycerin include hydrocarbons such as serine, paraffin wax, and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0027] Examples of the surfactant include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of usable surfactants include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkyl benzyl ammonium salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethyl imidazolinium salts, N,N-dialkyl morpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethyl ammonio betaine, N,N,N-trialkyl-N-alkylene ammonio carboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropyl ammonio sulfobetaine, and the like.

[0028] Examples of emulsifiers and emulsification aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, soy-derived water-soluble polysaccharides, soy-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), and the like.

[0029] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extracts, estradiol, various amino acids and their derivatives.

[0030] Examples of thickening agents include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin, pullulan, and aloe polysaccharides; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0031] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0032] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. These include kilisoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, fermented radish liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0033] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0034] Anti-acne agents include, for example, sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0035] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybeans, adzuki beans, etc.), and the like.

[0036] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0037] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0038] Examples of sequestering agents include disodium edetate, trisodium edetate, tetrasodium edetate, etidronic acid or a salt thereof (e.g., tetrasodium etidronic acid), gluconic acid or a salt thereof (e.g., sodium gluconate), diethylenetriaminepentaacetic acid or a salt thereof (e.g., pentasodium diethylenetriaminepentaacetic acid), hydroxyethanediphosphonic acid or a salt thereof (e.g., tetrasodium hydroxyethanediphosphonic acid), and phytic acid or a salt thereof (e.g., sodium phytate).

[0039] Examples of pigmentation prevention agents include one or more selected from kojic acid or its derivatives, ascorbic acid or its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, vitamin E or its derivatives, nicotinic acid or its derivatives, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), placenta extract, and linoleic acid.

[0040] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and L-ascorbic acid 2-sulfate. ascorbic acid ester salts such as tetraisopalmitate and tetraisopropylmagnesium ascorbic acid, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid) and L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, ... Examples of the hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of the resorcinol derivatives include, for example, 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc.; examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate; examples of vitamin E derivatives include vitamin E nicotinate and vitamin E linoleate; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0041] Anti-wrinkle agents include vitamin A or its derivatives, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, magnesium ascorbyl phosphate, etc.), pantothenyl alcohol, tranexamic acid, and niacinamide.

[0042] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms. For example, collagen or a hydrolysate thereof, yeast extract or hydrolysate, lactic acid bacteria culture, Gramineae plants, Cruciferae plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraeae plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of such extracts include extracts of one or more plants selected from the family Gardenia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Laminaceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0043] As the components derived from plants of the Gramineae family, in particular, hydrolyzed rice leaves, hydrolyzed rice extracts, hydrolyzed rice bran extracts, hydrolyzed germinated brown rice, fermented rice liquid, sake lees extracts derived from sake, and bamboo shoot skin extracts of Madake or Moso bamboo are preferred. As the components derived from plants of the Brassicaceae family, white mustard extracts or hydrolyzates thereof, and fermented white mustard are preferred. As the components derived from plants of the Theaceae family, in particular, green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, honey-scented black tea, etc.) are preferred. As the components derived from plants of the Rosaceae family, extracts of Damask rose flowers, extracts of peach flowers, leaves or immature fruits, extracts of strawberry flowers, extracts of cherry blossoms or leaves, and extracts of apricot fruits or seeds are preferred. As the component derived from a plant of the family Paeoniaceae, an extract of the root or flower of the peony and an extract of the flower or root of the peony are preferred. As the component derived from a plant of the family Amaranthaceae, an extract of Salicornia herbacea is particularly preferred. As the component derived from a plant of the family Zosteraea, an extract of Zostera marina or Zostera koa is particularly preferred. As the component derived from a plant of the family Fabaceae, an extract of white soybean or black soybean or a hydrolyzate thereof, or a fermented soy milk liquid, an extract of adzuki bean, an extract of red clover, and an extract of pueraria lobata root are particularly preferred. As the component derived from a plant of the family Asteraceae, an extract of burdock root, an extract of sunflower sprouts, an extract of Acanthus gracilis, an extract of Arnica officinalis, or an extract of chamomile flower are particularly preferred. As the component derived from a plant of the family Malvaceae, a fermented product of hibiscus, Rose of Sharon, or Hibiscus is preferred. As the component derived from a plant of the family Gentianaceae, an extract of gentiana is preferred. As the plant of the family Lamiaceae, an extract of perilla frutescens and an extract of the fruit of Acanthus purpurea are preferred. As the component derived from a plant of the Nelumbinaceae family, particularly preferred is a lotus flower or lotus seed extract or a fermented lotus seed product. As the component derived from a plant of the Cucurbitaceae family, particularly preferred is a loofah extract. As the component derived from a plant of the Araliaceae family, a ginseng extract or fermented product is preferred. As the component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) is included. As the component derived from a plant of the Bignoniaceae family, a Pau d'Arco bark extract is preferred. As the component derived from a plant of the Actinidiaceae family, an unripe kiwi extract is preferred.As the component derived from a plant of the family Mulberry, extract of mulberry fruit, and extract of fruit or bark of fig are preferable. As the component derived from a plant of the family Rhamnaceae, extract of jujube fruit is preferable. As the component derived from a plant of the family Iridaceae, saffron is preferable. As the component derived from a plant of the family Campanulaceae, extract or hydrolysate of root of Codonopsis arvensis is preferable. As the component derived from a plant of the family Anacardiaceae, mango fruit extract is particularly preferable. As the component derived from a plant of the family Garcinia, mangosteen fruit extract is particularly preferable. As the component derived from a plant of the family Valenciaceae, cherimoya fruit extract is preferable. As the component derived from a plant of the family Rutaceae, extract of Satsuma mandarin, bergamot fruit, extract of grapefruit or Banpeiyu fruit (including immature fruit), extract containing flavonoids and their glycosides contained in plants such as grapefruit or Hassaku, or extract of Japanese pepper seed are preferable. As the component derived from a plant of the Liliaceae family, an extract of Daylily, Daylily, Casablanca, Madonna lily, or Lilium longiflorum is preferred. As the component derived from a plant of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferred. As the component derived from a plant of the Oleaceae family, an extract of Jasmine flower is particularly preferred. As the plant of the Cupressaceae family, an extract of Juniperus communis fruit is particularly preferred. As the component derived from a plant of the Myrtaceae family, an extract of guava leaf is particularly preferred. As the plant of the Orchidaceae family, an extract of Bletilla serrata root (white orchid) is particularly preferred. As the component derived from a plant of the Convolvulaceae family, an extract of sweet potato or a fermented product thereof, or an extract of sweet potato shochu lees or a fermented product thereof is preferred. As the component derived from a plant of the Asparagaceae family, an extract of asparagus is particularly preferred. As a component derived from seaweed of the Laminaria family, a kelp extract is particularly preferred, as a component derived from seaweed of the Laminaria family, a katamen kirinsai extract is particularly preferred, as a component derived from seaweed of the Ulvaceae family, an Ulva pertusa extract is particularly preferred, and as a component derived from seaweed of the Funoriaceae family, a funori extract is particularly preferred.

[0044] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples and Test Examples, but the present invention is not limited thereto. In the following, all parts are by weight and all % are by weight.

[0045] Production Example 1. Preparation of Rutaceae Plant Extract (1) The peel of bitter orange (Citrus genus) was dried, and 500 g of 1,3-butylene glycol and 500 g of purified water were added to 100 g of the dried product, followed by stirring and extraction for 3 hours at 60° C. After the extraction operation, the crude extract was filtered to obtain 1,040 g of a light yellow-brown bitter orange peel extract (solid content concentration 2.34%).

[0046] Production Example 2. Preparation of Rutaceae Plant Extract (2) In Production Example 1, 1,010 g of a light yellow-brown bitter orange peel extract was obtained by the same procedure as in Production Example 1, except that 1,000 g of purified water was used as the extraction solvent instead of 500 g of 1,3-butylene glycol and 500 g of purified water (solid content concentration: 2.20%).

[0047] Preparation Example 3. Preparation of Rutaceae Plant Extract (3) In Production Example 1, 1,045 g of a light yellow-brown bitter orange peel extract was obtained by the same procedure as in Production Example 1, except that 500 g of ethanol and 500 g of purified water were used as the extraction solvent instead of 500 g of 1,3-butylene glycol and 500 g of purified water. (Solid content concentration: 2.35%)

[0048] Preparation Example 4. Preparation of Rutaceae plant extract (4) The bark of Phellodendron amplexicaule (Rutaceae) was dried, and 500 g of purified water and 500 g of 1,3-butylene glycol were added to 120 g of the dried product, followed by extraction for 2 hours at 40° C. This was filtered to obtain 750 g of a yellow Phellodendron amplexicaule bark extract (solid content concentration 0.98%).

[0049] Preparation Example 5. Preparation of Rutaceae Plant Extract (5) In Production Example 4, 730 g of yellow Phellodendron amurense bark extract was obtained (solid content concentration 0.95%) by the same procedure as in Production Example 4, except that 1000 g of purified water was used instead of 500 g of purified water and 500 g of 1,3-butylene glycol as the extraction solvent.

[0050] Preparation Example 6. Preparation of Rutaceae Plant Extract (6) In Production Example 4, 765 g of yellow Phellodendron amurense bark extract was obtained (solid concentration 1.01%) by the same procedure as in Production Example 4, except that 500 g of purified water and 500 g of ethanol were used as the extraction solvent instead of 500 g of purified water and 500 g of 1,3-butylene glycol.

[0051] Test Example 1. Evaluation of the effect of promoting the expression of filaggrin and peptidylarginine deiminase-1 Normal human epidermal cells (NHEK) were cultured in a 24-well plate at 3 × 10 4The cells were seeded at 1000 cells / well and cultured at 37°C under 5% CO2 and saturated steam. Test sections were set up to add the extracts of Production Examples 1 and 4 as sample solutions, and after 24 hours of culture, each sample solution was added and cultured for another 48 hours. The concentration of each sample solution was added so that the final concentration as a solution was 1.0% relative to the amount of medium. As a comparison control, a control section was set up in which a culture solution containing only 30% BG or 50% BG solution of the same concentration was added instead of the above sample solutions, and cultured in the same manner. Next, the cells in each test section and control section were recovered with 0.5 mL of ISOGEN II reagent (manufactured by Nippon Gene Co., Ltd.). 200 μL of RNase-free water was added to the recovered cells, which were stirred and mixed, left at room temperature for 15 minutes, and then centrifuged for 15 minutes at 15,000 rpm and 4°C using a centrifuge (manufactured by TOMY Co., Ltd. / MX-160), and 500 μL of the supernatant was taken out. 500μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected supernatant, stirred and mixed, left at room temperature for 10 minutes, and then centrifuged at 15,000 rpm and 4℃ for 10 minutes to obtain a precipitate of total RNA. 0.5mL of 75% ethanol was added to the total RNA, and the precipitate was collected by centrifugation at 15,000 rpm and 4℃ for 3 minutes. The supernatant was discarded, and this process was repeated twice. After completely removing the supernatant, the mixture was air-dried and dissolved in RNase-free water. The collected total RNA was reverse-transcribed using a designated kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [Takara Bio]) to synthesize cDNA. Using the synthesized cDNA as a sample, the expression of various genes and the expression of the internal standard GAPDH gene were detected using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM (Perfect Real Time) [manufactured by Takara Bio Inc.].Here, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is one of the housekeeping genes (a gene that is expressed at a constant level in many tissues and cells, is constantly expressed, and is essential for the maintenance and proliferation of cells), and since its expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of each gene in each test group when the expression level of the GAPDH gene was kept constant. The expression level in the control group was set to 100% and a relative value was calculated.

[0052] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG2025004742000002.jpg62115

[0053] As shown in Table 1, this Test Example 1 confirmed that the extract of Production Example 1 according to the present invention significantly promotes the expression of filaggrin, and the extract of Production Example 4 according to the present invention significantly promotes the expression of an enzyme called peptidylarginine deiminase-1 (PADI1). In the stratum corneum, filaggrin is deiminated by PADI1 to become deiminated filaggrin, and this deiminated filaggrin is decomposed by enzymes to produce amino acids (NMFs) that are natural moisturizing factors. Therefore, by applying a combination of one or more of the extracts of Production Examples 1 and 4 according to the present invention to the skin, it is possible to promote the production of natural moisturizing factors by skin cells themselves, provide a sustained feeling of moisture, and furthermore, continuously improve transparency and luster.

[0054] Test Example 2: Evaluation test of the effect of promoting tight junction synthesis Normal epidermal cells (NHEK) were cultured in a 96-well plate at 1.5 x 10 4The cells were seeded at 1.0 cells / well and cultured at 37°C under 5% CO2 and saturated water vapor. The cells were cultured in the wells for 48 hours to make them subconfluent. Next, a sample solution (the extract of Production Example 1) was added, and the cells were cultured for another 48 hours. The concentration of the sample solution was added so that the final concentration as a solution was 1.0% relative to the amount of medium. In addition, as a comparative control, a control group was set in which a culture solution containing only 30% BG or 50% BG solution of the same concentration was added instead of the sample. After completion, the culture solution was removed, the cells were washed with D-PBS (containing no Ca or Mg, hereinafter referred to as PBS), and PBS containing 10% trichloroacetic acid was added as a fixative, and the cells were allowed to stand at 4°C for 30 minutes. After removing the fixative and washing with PBS, PBS containing 0.2% Triton-X (hereinafter referred to as wash solution) was added and incubated at room temperature for 1 hour. Then, the cells were incubated overnight at 4°C using Blocking One P (manufactured by Nacalai Tesque) as a blocking agent. After washing once with washing solution, anti-occludin (Life Span Bio Sciences) was diluted 500-fold and added as a primary antibody, and further incubated at 4°C overnight. After washing three times with washing solution, Alexa Fluor (registered trademark) 488 Goat Anti-mouse IgG (Invitrogen) was diluted 500-fold and added as a secondary antibody. After incubation at room temperature for 2 hours, washing was performed five times with washing solution. Then, Hoechst 33342 (Dojindo Laboratories) was diluted 1000-fold with PBS and added, and incubated for 30 minutes. The fluorescence intensity of Alexa Fluor (registered trademark) 488 and Hoechst 33342 was measured with Fluoroskan Ascent (ThermoLabsystems), and the fluorescence intensity of Alexa Fluor (registered trademark) 488 was corrected using the value of Hoechst 33342 as an internal standard, and the relative value was calculated with each detection value set to 100% of the control area.

[0055] The results of Test Example 2 are shown in Table 2. [Table 2] JPEG2025004742000003.jpg31114

[0056] As shown in Table 2, Test Example 2 confirmed that the extract of Production Example 1 of the present invention exhibits the effect of promoting the synthesis of a protein (occludin) that constitutes tight junctions, which are binding structures between cells. This suggests that the extract of the present invention can improve the barrier function of the skin and prevent evaporation of moisture from the skin, thereby providing the skin with a sustained moist feeling. It also suggests that the extract exhibits the effect of preventing the invasion of bacteria and foreign substances (environmental pollutants, etc.) into the skin from the outside.

[0057] Test Example 3. Evaluation test of prostaglandin E2 (PEG2) synthesis inhibition Normal human epidermal cells (NHEK) were cultured in a 96-well plate at 8 × 10 3 The cells were seeded at 100 cells / well and cultured at 37°C under 5% CO2 and saturated steam. After 24 hours of culture, the extract of Production Example 4 was added as a sample solution for evaluation, and cultured for another 24 hours. As a comparative control, a test group (control group) was set in which a culture solution containing only 30% BG or 50% BG solution of the same concentration was added instead of the sample solution. Next, the medium was replaced with one not containing the evaluation sample, and approximately 50 mJ / cm was irradiated from the bottom of the culture vessel using a UV-B lamp (TL20W / 12RS, manufactured by Philips). 2 The medium was then replaced with one containing the evaluation sample, and the cells were cultured for 24 hours, after which the supernatant was collected. The amount of PGE2 in the collected supernatant was measured using a Prostaglandin E2 ELISA Kit - Monoclonal (Cayman Chemical Company) according to the manual. The difference between the measured values ​​in the UV-B irradiated and unirradiated control groups was taken as an inhibition rate of 100%, and the inhibitory effect of the evaluation sample on PGE2 metabolism was calculated as a relative value.

[0058] The results of Test Example 3 are shown in Table 3. [Table 3] JPEG2025004742000004.jpg31114

[0059] As shown in Table 3, it was confirmed by Test Example 3 that the extract of Production Example 4 according to the present invention significantly inhibits the metabolism of prostaglandin E2, which is one of the physiologically active substances involved in skin inflammation. As a result, the extract of Production Example 4 according to the present invention can suppress inflammation, improve skin texture, suppress redness, and improve luster, and can also suppress pigmentation caused by inflammation. In addition, by combining with the extract of Production Example 1 according to the present invention, it is possible to synergistically exhibit the effect of promoting the expression of filaggrin and PADI1, and the effect of promoting the synthesis of proteins that constitute tight junctions, and it is also suggested that a synergistic effect of continuously improving the moisturizing, translucent, and luster of the skin can be exhibited.

[0060] Test Example 4. Evaluation test of increased activity of oxidized ascorbate reductase [Sample preparation] In Test Example 4, the extract of Production Example 1, the extract of Production Example 4, and a mixture of the extract of Production Example 1 and the extract of Production Example 4 (hereinafter referred to as "Mixture 1") were prepared as sample solutions. As described below, these sample solutions were adjusted to 1.0% each (in the case of Mixture 1, the total of the mixed extracts was adjusted to 1.0%). [Evaluation test procedure] NHEK epidermal cells were cultured in HuMedia KG2 medium (Kurabo) at 8.0 × 10 4The cells were seeded at 1000 cells / well, and cultured for 24 hours under conditions of 37°C and 5% CO2. The medium was replaced with HuMedia KB2 medium (Kurabo) containing each sample solution, and cultured for 48 hours under the same conditions. Here, the final concentration of each sample solution was adjusted to 1.0% relative to the amount of medium. After the culture was completed, the cells were detached using trypsin and collected in a microtube using PBS(-) containing 5% FBS. After that, the cells were washed twice with PBS(-), the supernatant was removed, and the cells were rapidly frozen using liquid nitrogen and stored at -80°C until the enzyme activity was measured. In this test example 4, the activity of an enzyme that reduces oxidized ascorbic acid (dehydroascorbic acid) (hereinafter referred to as "DHA reductase") was measured. The DHA reductase activity was measured as follows. 500 μL of Passive Lysis Buffer (Promaga) was added to the stored cells, and the cells were dissolved by pipetting. After centrifugation (12000g x 5 min, 4°C), the supernatant was collected and used as a crude enzyme solution. 50 μL of the obtained crude enzyme solution was added per well, and 50 μL of purified water (control) or 50 μL of 2 mM dehydroascorbic acid solution (test) was added. 50 μL of DTNB (5,5'-dithio-bis- (2-nitrobenzoic acid)) test solution (CAYMAN CHEMICAL COMPANY) was added, and the absorbance at 414 nm was measured. The difference obtained by subtracting the value of the test group from the value of the control group for each sample addition group was used as the DHA reductase activity. Meanwhile, the protein concentration of the crude enzyme solution was measured by the Bradford method using Protein Assay Dye (BIO-RAD), and the value obtained by dividing the absorbance difference by the protein concentration was used as the DHA reductase activity per protein for each sample addition group, and the relative value was calculated by taking the value of the solvent control addition group as 100.

[0061] The results of Test Example 4 are shown in Table 4. [Table 4] JPEG2025004742000005.jpg67107

[0062] As shown in Table 4, Test Example 4 confirmed that Extracts 1 and 4 according to the present invention activate DHA reductase. Furthermore, as shown in Table 4, it was also confirmed that the combination of the extract of Production Example 1 and the extract of Production Example 4 further activates DHA reductase. As a result, Extracts 1 and 4 according to the present invention can reduce oxidized ascorbic acid (dehydroascorbic acid) that has been oxidized in the body and lost its functions such as antioxidant effect, returning it to the original ascorbic acid, and promoting a more sustained exertion of its functions.

[0063] Test Example 5. Evaluation test of epidermal cell activation effect Human epidermal cells (NHEK) were cultured at 8 × 10 in a 96-well microplate containing HuMedia KG2 medium (Kurabo). 3 The cells were seeded at 1000 cells / well, pre-cultured for 1 day under conditions of 37°C and 5.0% CO2, and then the medium was replaced with HuMedia KB2. PBS(-) containing the extract of Production Example 1 as a sample solution was added to the medium (prepared so that the final concentration of the solution was 1.0%), and the medium was cultured for another 2 days under the same conditions. After removing the medium, Hoechst33342 was added to perform DNA staining, and measurements were performed at Ex=355nm and Em=460nm using a fluorescent plate reader (Fluoroscan Ascent, Thermo Fisher Scientific). After the measurements, 0.03% MTT was added and the plate was kept at 37°C for 1 hour, after which the generated formazan was extracted with isopropanol, and the MTT value was measured at wavelengths of 570-630nm using a microplate reader (Model 680, Bio-Rad). The same procedure was carried out for the control case where the same concentration of 1,3-butylene glycol was added instead of the sample solution, and the MTT activity per epidermal cell was calculated by dividing the obtained MTT value by the amount of DNA. The obtained MTT activity per epidermal cell was expressed as a relative value with the control set at 100.

[0064] The results of Test Example 4 are shown in Table 5. [Table 5] JPEG2025004742000006.jpg45120

[0065] As shown in Table 5, it was confirmed that the extract of Production Example 1 according to the present invention has an epidermal cell activation effect. This effect suggests that the extract of Production Example 1 according to the present invention promotes the energy metabolism of epidermal cells and regulates epidermal turnover. It is also suggested that regulating epidermal turnover improves the skin's moisturizing properties and repairs open pores by plumping up the skin.

[0066] Test Example 6. Evaluation test of fibroblast activation effect Human dermis-derived fibroblasts, NB1RGB, were cultured in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium at 1 × 10 4 After seeding, the cells were pre-cultured for 1 day under conditions of 37°C and 5.0% CO2, and the extract of Production Example 1 according to the present invention was added to the medium as a sample solution (prepared so that the final concentration of the solution was 1.0%). After adding the sample solution, the cells were further cultured for 3 days under the same conditions as the pre-culture. Next, after removing the medium, Hoechst33342 was added to perform DNA staining, and measurements were performed at Ex=355nm and Em=460nm using a fluorescent plate reader (Fluoroscan Ascent, Thermo Fisher Scientific). After the measurements, 0.03% MTT was added and the cells were kept at 37°C for 1 hour, after which the generated formazan was extracted with isopropanol, and the MTT value was measured at a wavelength of 570-630nm using a microplate reader (Model 680, Bio-Rad). The same procedure was carried out for the control case where the same concentration of 1,3-butylene glycol was added instead of the sample solution, and the MTT activity per cell was calculated by dividing the MTT value obtained by the amount of DNA. The MTT activity per cell obtained was expressed as a relative value with the control set at 100.

[0067] The results of Test Example 6 are shown in Table 6. [Table 6] JPEG2025004742000007.jpg45121

[0068] As shown in Table 6, Production Example 1 according to the present invention has a fibroblast activation effect, suggesting that it activates energy metabolism in the dermis, thereby maintaining the dermis in a healthy state, inhibiting a decrease in skin elasticity, and preventing and improving wrinkles.

[0069] Test Example 6: Evaluation test of collagen synthesis promotion effect Human dermis-derived fibroblasts, NB1RGB, were cultured in a 96-well microplate containing 0.5% NCS-containing Eagle's minimum essential medium at 1 × 10 4 After seeding at 1000 cells / well and pre-cultivating for 1 day under conditions of 37°C and 5.0% CO2, the extract of Production Example 1, the extract of Production Example 4, and the mixture 1 used in Test Example 4 were added to the medium as sample solutions. Here, the sample solution concentrations were set to 1.0% for the extract of Production Example 1, 0.5% for the extract of Production Example 4, and the mixture 1 was set to 1.5% of the extract of Production Example 1 (1.0%) and the extract of Production Example 4 (0.5%), and the test group was set. After adding each sample solution, the cells were cultured for another 5 days under the same conditions as the pre-cultivation conditions using each medium. Next, the medium was removed, the cells were fixed with cold methanol and cold ethanol, and then stained with a saturated aqueous picric acid solution containing 0.1% Sirius Red. After washing with purified water, extraction was performed with a 0.1% NaOH:methanol=1:1 solution, and the collagen amount was measured at a wavelength of 540 nm using a microplate reader (Model 680, Bio-Rad). Furthermore, for comparison, the same procedure was carried out for the control without the addition of the sample but with the same concentration of 1,3-butylene glycol, and the relative value of the collagen amount obtained when each sample solution was added to the control was calculated, and this was taken as the collagen synthesis rate (%). In addition, the collagen synthesis rate was also measured when magnesium ascorbyl phosphate (APM) was used as a positive control.

[0070] The results of Test Example 6 are shown in Table 6. [Table 6] JPEG2025004742000008.jpg68121

[0071] As shown in Table 6, it was confirmed that the extracts according to the present invention (extracts of Production Example 1, Production Example 4, and Mixture 1, which is a combination of the extracts of Production Examples 1 and 4) significantly promote collagen synthesis in fibroblasts. This suggests the effect of suppressing loss of skin elasticity and preventing and improving wrinkles.

[0072] Test Example 7. Evaluation test of the effect of inhibiting NF-κB activity Normal epidermal cells (NHEK) were placed in a 96-well plate at 8.0 × 10 4 The cells were seeded at 100 cells / well and cultured at 37°C under 5% CO2 and saturated steam. The extract of Production Example 4 (final concentration as solution: 0.5%) was then added as a sample solution, and the cells were further cultured for 48 hours. As a comparative control, a test group (control group) was set up in which a culture solution containing only 1,3-butylene glycol at the same concentration was added instead of the sample solution. The cells were exposed to ultraviolet light (UV-B) of 100 mJ / cm2. 2 After irradiation so that the cells were exposed to light, the cells were cultured for 6 hours. The culture medium was removed, the cells were washed with D-PBS (containing no Ca or Mg, hereinafter referred to as PBS), and 10% formalin was added as a fixative, and the cells were allowed to stand at room temperature for 60 minutes. After removing the fixative and washing with PBS, PBS containing 0.2% Triton-X (hereinafter referred to as washing solution) was added and incubated at room temperature for 1 hour. Then, the cells were incubated at room temperature for 1 hour using Blocking One P (manufactured by Nacalai Tesque) as a blocking agent. After washing once with washing solution, anti-NF-κB antibody (manufactured by GeneTex) was diluted 200 times and added as a primary antibody, and further incubated overnight at 4°C. After washing three times with washing solution, Alexa Fluor (registered trademark) 546 Goat Anti-rabbit IgG (manufactured by Invitrogen) was diluted 500 times and added as a secondary antibody. After incubation at room temperature for 2 hours, the cells were washed five times with washing solution. Then, images were taken using a fluorescence microscope (OLYMPUS, IX71), and the transfer of the fluorescent signal from the cytoplasm into the nucleus was used as an indicator.

[0073] NF-κB, whose activity was evaluated in Test Example 7, is known as a transcription factor that acts during inflammatory reactions in cells, and has been reported to respond to ultraviolet light and the like. NF-κB is usually localized in the cytoplasm, but is known to rapidly migrate into the nucleus and become activated in response to ultraviolet light and to be involved in the expression of various genes as a transcription factor. In Test Example 7, whether NF-κB is localized in the nucleus was evaluated as an indicator of NF-κB activation. The results of Test Example 7 are shown in Figure 1. The arrow in Figure 1 indicates a cell in which NF-κB can be confirmed to be localized only in the nucleus, and therefore the extract of the present invention (the extract of Production Example 4) was shown to have the effect of suppressing the nuclear migration of NF-κB caused by ultraviolet light. Therefore, according to the present invention, by suppressing the migration of NF-κB to the nucleus, inflammation can be suppressed, skin texture can be improved, redness can be suppressed, and luster can be improved. Furthermore, by combining it with the extract of Example 1 of the present invention, it is possible to exert a synergistic effect of promoting the expression of filaggrin and PADI1, and promoting the synthesis of proteins that constitute tight junctions, suggesting that it exerts a synergistic effect of sustainably improving the moisture, transparency and luster of the skin.

[0074] Examples of skin external preparations containing the extracts of Preparations 1 to 3 and 4 to 6 according to the present invention are shown below, but the present invention is not limited thereto. For example, skin lotions, milky lotions, creams, beauty essences, packs and sheet masks are expected to exert a synergistic effect of continuously improving moisturization, transparency and luster in the epidermis, and to exert an effect of repairing open pores, and in the dermis, they are expected to exert an effect of activating fibroblasts and promoting collagen synthesis, thereby suppressing the loss of skin elasticity and preventing and improving wrinkles.

[0075] Formulation example 1. Lotion [Ingredients] Part Extract of Preparation Example 1 2.0 Squalane 0.2 Mandarin Orange Oil 0.01 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Isopropyl methylphenol 0.1 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water (enough to make the total amount 100 parts)

[0076] Formulation example 2: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 2 was used instead of 2.0 parts of the extract of Production Example 1.

[0077] Formulation example 3: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 3 was used instead of 2.0 parts of the extract of Production Example 1.

[0078] Formulation example 4: Lotion [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Squalane 0.2 Mandarin Orange Oil 0.1 Bitter Orange Flower Oil 0.1 Lemon peel oil 0.1 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Ascorbyl Palmitate 0.1 Pentylene glycol 0.1 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water (enough to make the total amount 100 parts)

[0079] Formulation example 5. Lotion [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Mandarin Orange Oil 0.1 Cetanol 2.0 Behenyl Alcohol 2.0 Niacinamide 5.0 Dipotassium glycyrrhizinate 0.1 Glycerin 1.0 1,3-Butylene glycol 1.0 Pentanediol 1.0 Potassium hydroxide 0.5 Purified water (enough to make the total amount 100 parts)

[0080] Formulation example 6. Lotion [Ingredients] Part Extract of Preparation Example 2 1.0 Extract of Preparation Example 5 0.25 Avocado oil 0.1 Olive oil 0.1 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Ascorbic Acid Glucoside 2.0 Niacinamide 5.0 ε-Aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Pyridoxine hydrochloride 0.5 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total amount 100 parts)

[0081] Formulation example 7. Emulsion [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl Dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Ascorbic acid phosphate magnesium salt 3.0 Mandarin Orange Oil 0.01 Arbutin 3.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropyl methylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Acetyl hyaluronic acid 0.1 Disodium edetate 0.05 Diethylenetriaminepentaacetic acid pentasodium 0.05 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0082] Formulation example 8. Emulsion An emulsion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of magnesium ascorbyl phosphate.

[0083] Formulation example 9. Emulsion An emulsion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of tranexamic acid and 0.05 parts of sodium metabisulfite were used in place of 2.0 parts of magnesium ascorbyl phosphate.

[0084] Formulation example 10. Emulsion An emulsion was obtained in the same manner as in Formulation Example 7, except that 3.0 parts of 3-O-ethyl ascorbic acid was used in place of 2.0 parts of magnesium ascorbyl phosphate among the ingredients of Formulation Example 7.

[0085] Formulation example 11. Emulsion An emulsion was obtained in the same manner as in Formulation Example 7, except that 2.0 parts of ascorbic acid phosphate magnesium salt and 5.0 parts of niacinamide were used in place of potassium hydroxide.

[0086] Formulation example 12. Cream [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Olive oil 5.0 Mandarin Orange Oil 1.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.0 Candelilla Wax 1.0 Lactic acid fermented rice 2.0 Soybean-derived hydrogenated lecithin 0.5 Carboxyvinyl Polymer 1.0 Sodium alginate 1.0 Glycerin 2.0 Pentanediol 1.0 PH adjuster (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0087] Formulation example 13. Cream [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.25 Olive oil 5.0 Jojoba oil 5.0 Mandarin Orange Oil 1.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Di(octyldodecyl) lauroyl glutamate / phytosteryl / Behenyl) 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-Oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Niacinamide 5.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Hydrolyzed Collagen 1.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-Camphor 0.3 l-Menthol 0.5 Purified water (enough to make the total amount 100 parts)

[0088] Example 14. Pack [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Water-soluble collagen 1.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total amount 100 parts)

[0089] Formulation example 15. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following components. [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Niacinamide 1.0 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 0.1 Water-soluble collagen 0.1 Sodium hyaluronate 0.01 Glycerin 1.0 1,3-Butylene glycol 2.0 Pentanediol 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0090] Formulation example 16. Beauty serum [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Sodium hyaluronate 1.0 Water-soluble collagen 1.0 Tranexamic acid 0.1 Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Citric acid 0.3 Sodium citrate 0.6 Eucheuma muscaria extract 5.0 Purified water (enough to make the total amount 100 parts)

[0091] Prescription example 17. Cream [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.25 Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Shea Butter 2.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Niacinamide 1.0 Lactic acid fermented rice 3.0 Hydrogenated Lecithin 2.0 Eucheuma muscaria extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0092] In addition, the extracts of Preparations 1 to 3 and 4 to 6 according to the present invention can also be used as skin preparations for washing the face and other parts of the arms and torso, as preparations for washing the hair and scalp, and as preparations for protecting the hair, as described below. By blending the extracts of Preparations 1 to 3 and 4 to 6 according to the present invention, it is expected that the effect of maintaining the moisture of washed skin (scalp) and hair can be achieved.

[0093] Formulation example 18. Cleansing cosmetics [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Potassium cocoyl glycine 5.0 Glycerin 10.0 Glyceryl Caprylate 1.0 Sodium lauroyl aspartate 10.0 Water-soluble collagen 5.0 Cetanol 3.0 Myristyl Alcohol 3.0 Isopropyl methyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Dipotassium glycyrrhizinate 0.1 Stearyl Glycyrrhetinate 0.1 Tocopherol acetate 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Sodium citrate (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0094] Prescription example 19. Hair shampoo [Ingredients] Part Extract of Preparation Example 1 2.0 Extract of Preparation Example 4 1.0 Sodium Laureth Sulfate 10.0 Glyceryl Monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl Alcohol 2.0 Behenyl Alcohol 2.0 Dimethicone 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium Salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water (enough to make the total amount 100 parts)

[0095] Formulation example 20. Hair conditioner [Ingredients] Part Extract of Preparation Example 1 1.0 Extract of Preparation Example 4 0.5 Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Allantoin 0.1 Isopropyl methylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water (enough to make the total amount 100 parts)

Claims

1. An external skin preparation for improving the moisturizing, clarity and luster of the skin, comprising as an active ingredient an extract of one or two plants belonging to the Rutaceae family, Citrus aurantium and Phellodendron amurense Ruprecht.

2. An activator of oxidized ascorbic acid reductase, comprising as an active ingredient an extract of one or two plants belonging to the Rutaceae family, namely, Citrus aurantium and Phellodendron amurense Ruprecht.

3. An external skin preparation for inhibiting loss of skin elasticity and preventing and improving wrinkles, comprising as active ingredients an extract of one or two plants belonging to the Rutaceae family, Citrus aurantium and Phellodendron amurense Ruprecht.

4. A skin topical agent for improving pores, comprising as an active ingredient an extract of one or two plants belonging to the Rutaceae family, Citrus aurantium and Phellodendron amurense Ruprecht.

5. A skin topical agent containing as active ingredients an extract of bitter orange (Citrus aurantium), which belongs to the Rutaceae family, and an extract of yellow hawthorn (Phellodendron amurense Ruprecht).

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