Composition

Abstract

To provide a composition with a significant anti-glycation action.SOLUTION: A composition contains specific materials.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition having an excellent anti-glycation effect. [Background technology]

[0002] The glycation reaction of proteins, in which the amino groups of amino acids and proteins react nonenzymatically and irreversibly with reducing sugars to produce advanced glycation endproducts (hereinafter abbreviated as AGEs), is known as the Maillard reaction, and methylguanidine is known as a representative inhibitor of this reaction.

[0003] In recent years, it has become clear that protein glycation occurs in vivo and is the cause of various aging phenomena such as diabetes, arteriosclerosis, and aging-related skin sagging, wrinkles, and dullness. Although pharmaceuticals that have anti-glycation effects by inhibiting the production of AGEs have been developed (Non-Patent Document 1), they are known to have side effects. [Prior art documents] [Non-patent literature]

[0004] [Patent Document 1] Am J Nephrol.2004;24,32-40. Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a composition that can be used safely and on a daily basis and has an excellent anti-glycation effect. [Means for solving the problem]

[0006] The present inventors have discovered that a combination of specific materials provides superior anti-glycation effects compared to the use of these materials alone, and have thus completed the present invention.

[0007] That is, the present invention is as follows. [1] A composition comprising a polyamine or a salt thereof and at least two selected from chrysanthemum, safflower, rose, pomegranate, barley, and mugwort. [2] A composition containing a polyamine or a salt thereof and at least one selected from chrysanthemum, safflower, barley, and mugwort. [3] The composition according to [2], comprising a polyamine or a salt thereof, and at least one selected from chrysanthemum, safflower, barley, and mugwort in a ratio of 1:10 to 5000. [4] A composition containing chrysanthemum and at least one selected from safflower, rose, and pomegranate. [5] The composition according to [4], comprising chrysanthemum and at least one selected from safflower, rose and pomegranate in a ratio of 1:0.01 to 100. [6] A composition containing a polyamine or a salt thereof, chrysanthemum and safflower. [7] The composition according to any one of [1] to [6], which is an anti-glycation composition. [8] The composition according to any one of [1] to [6], which is a cosmetic composition. [9] The composition according to any one of [1] to [8], which is an oral composition.

[10] The composition according to any one of [1] to [8], which is a cosmetic composition. Effect of the Invention

[0008] The composition of the present invention, by combining specific materials, has a remarkable anti-glycation effect on proteins, etc. Furthermore, since preventing the glycation of proteins, etc. and suppressing the production of AGEs leads to the inhibition of activation of the receptor for advanced glycation end products (Receptor for AGE; RAGE), which causes various symptoms due to glycation, the composition of the present invention can be an excellent anti-glycation composition or cosmetic composition. [Brief description of the drawings]

[0009] [Figure 1] FIG. 2 is a graph showing the glycation reaction inhibition rates according to Examples 1 and 2 and Comparative Examples 1 to 3. [Diagram 2]FIG. 2 is a graph showing the glycation reaction inhibition rates of Examples 3 to 4 and Comparative Examples 4 to 6. [Diagram 3] FIG. 1 is a graph showing the glycation reaction inhibition rates of Example 5 and Comparative Examples 7 and 8. [Figure 4] FIG. 1 is a graph showing the glycation reaction inhibition rates of Examples 6 and 7 and Comparative Examples 9 to 11. [Diagram 5] FIG. 1 is a graph showing the glycation reaction inhibition rates of Example 8 and Comparative Examples 12 to 13. [Figure 6] FIG. 1 is a graph showing the glycation reaction inhibition rates in Example 9 and Comparative Examples 14 to 15. [Figure 7] FIG. 1 is a graph showing the glycation reaction inhibition rates of Example 10 and Comparative Examples 16 to 17. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The composition of the present invention is characterized by containing at least two kinds selected from polyamine or its salt, chrysanthemum, safflower, pomegranate, rose, barley, and mugwort. In particular, it is preferable to contain a combination of polyamine or its salt and at least one kind selected from chrysanthemum, safflower, barley, and mugwort, or a combination of chrysanthemum and at least one kind selected from safflower, rose, and pomegranate, and it is even more preferable to contain three kinds of polyamine or its salt, chrysanthemum, and safflower, in terms of showing excellent anti-glycation action.

[0011] The composition of the present invention is expected to have an excellent AGE production inhibitory effect, i.e., glycation reaction inhibitory effect. In addition, the composition of the present invention is expected to have an effect of preventing and / or improving various symptoms caused by glycation, such as aging and symptoms that may affect beauty. Therefore, the composition of the present invention can be used as an anti-glycation composition or a cosmetic composition.

[0012] The materials used in the present invention will be described in detail below.

[0013] (Polyamine) In the present invention, the polyamine refers to an aliphatic carbohydrate having two or more primary amino groups, and examples thereof include diamines, triamines, tetraamines, pentaamines, and hexaamines. More specifically, examples of diamines include ethylenediamine, putrescine, cadaverine, hexamethylenediamine, ethambutol, and phenylenediamine. Examples of triamines include pentamethyldiethylenetriamine, trimethylenediamine, spermidine, cardine, homospermidine, and aminopropylcadaverine. Examples of tetraamines include spermidine. Examples of polyamines include pyridine, thermospermine, pyramine, aminopentylnorspermidine, aminopropylhomospermine, homospermine, canavalmine, pentaamines include caldopentamine, homocaldopentamine, aminopropylcanavalmine, bis(aminopropyl)homospermine, bis(aminopropyl)norspermine, aminobutylcanavalmine, aminopropylhomospermine, homopentamine, and hexamines include caldohexamine, homocaldohexamine, thermohexamine, and homosermohexamine. The composition of the present invention may contain multiple types of such polyamines. The composition of the present invention preferably contains one or more of spermidine, spermine, and putrescine, since they are present in the human body and therefore have high utilization efficiency, and exhibit high anti-glycation effects when used in combination with other specific materials, and among them, it is more preferable to contain spermine, which has the highest physiological activity due to the large number of amino groups. The polyamine of the present invention may also be in the form of a physiologically available salt.

[0014] Polyamines exist in all living organisms, and therefore can be isolated from raw materials such as tissues of various organisms. Specifically, plant materials such as rice, soybeans, peppers, oranges, bananas, and apples, or processed products thereof, animal materials such as meat and organs of fish such as salmon, and meat and organs of animals such as cows and pigs, or processed products thereof, or fungal materials such as mushrooms and yeast, or processed products thereof can be used. In the present invention, polyamines obtained by chemical synthesis or biochemical synthesis using enzymes or microorganisms can be used.

[0015] Examples of the processed form of the raw material containing polyamine include dried products, crushed products or their dried powders (hereinafter also referred to as "crushed powders"), squeezed products or their dried powders (hereinafter also referred to as "squeezed powders"), extracts or their dried powders (hereinafter also referred to as "extracted powders"), granules, pastes, etc. Examples of the solvent used for extraction include organic solvents such as water, methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, ethyl acetate, diethyl ether, and hexane, acidic aqueous solutions such as acetic acid, citric acid, or aqueous solutions thereof, and mixed solvents thereof. The temperature of the extraction solvent can be appropriately set from room temperature to below the boiling point depending on the solvent used.

[0016] As the raw material containing polyamine, it is preferable to use rice, soybean, mushrooms or yeast, more preferably rice, and particularly preferably rice germ. As the processed form of the raw material containing polyamine, it is preferable to use a crushed product or crushed powder, a squeezed product or squeezed powder, an extract or extract powder, more preferably an extract or extract powder, more preferably an extract or extract powder of an acidic aqueous solution, and particularly preferably an extract or extract powder of an aqueous citric acid solution. In the present invention, it is most preferable to use an aqueous citric acid extract of rice germ or an aqueous citric acid extract powder, since it shows an excellent anti-glycation effect when used in combination with other specific materials. In addition, the content of polyamine contained in the aqueous citric acid extract of rice germ is preferably 0.001% or more, more preferably 0.005% or more, particularly preferably 0.01% or more, and particularly preferably 0.05% or more, from the viewpoint of having an excellent anti-glycation effect when used in combination with other specific materials. The content of polyamine can be analyzed by high performance liquid chromatography (HPLC).

[0017] (chrysanthemum) The chrysanthemum in the present invention is a plant of the genus Chrysanthemum in the family Asteraceae, and its scientific name is Chrysanthemum morifolium Ramat. Chrysanthemum grows wild in Japan and is easily available, and its flowers have been used for medicinal purposes in China since ancient times. In Japan, it is also consumed as tea or alcohol, and is used as food in cooking, and is said to be effective against dizziness, headaches, eye fatigue, etc. Specific varieties include purple chrysanthemum, known as "Enmeiraku," yellow chrysanthemum, known as "Afang Palace," and "Small Chrysanthemum." Among these, it is preferable to use purple chrysanthemum, since it shows excellent anti-glycation effects when used in combination with other specific materials.

[0018] Chrysanthemum can be used as it is in the form of stems, leaves, fruits, pericarp, seeds, seed coats, roots, flowers, etc., but in the present invention, it is preferable to use flowers. In addition, in the present invention, various processed products of chrysanthemum can be used, and the processing form can be selected from the same processing form as the raw material containing polyamines. Among them, crushed products or crushed powder, squeezed products or squeezed juice powder, extracts or extract powders are preferred, and crushed products or crushed powders are particularly preferred. In the present invention, it is most preferable to use crushed products or crushed powder of flowers, since they show excellent anti-glycation effects when used in combination with other specific components.

[0019] (barley) The barley used in the present invention is a plant of the Gramineae family, and its scientific name is Hordeum vulgare. Examples of barley that can be used include two-row barley, six-row barley, and naked barley, and these can be used alone or in combination of two or more types.

[0020] The parts of barley used in the present invention include flowers, seeds, fruits, leaves, stems, and roots, but from the viewpoint of having an excellent anti-glycation effect when used in combination with other specific components, it is preferable to use one or more parts selected from leaves and stems (hereinafter sometimes referred to as stems and leaves). The stems and leaves are preferably harvested before maturity, that is, from the time when tillers start to before ear emergence.

[0021] In the present invention, various processed barley products can be used, and the processing form can be selected from the same processing forms as the raw materials containing the above polyamines. Among them, crushed products or crushed powder, squeezed products or squeezed powder, extracts or extract powders are preferred, and crushed products or crushed powders are particularly preferred. In the present invention, it is most preferred to use crushed products or crushed powders of barley stems and leaves, since they show excellent anti-glycation effects when used in combination with other specific components.

[0022] (Mugwort) In the present invention, mugwort is a perennial plant of the genus Artemisia in the family Asteraceae, and its scientific name is Artemisia princeps. The parts of mugwort used in the present invention include flowers, seeds, fruits, leaves, stems, and roots, but it is preferable to use stems and leaves from the viewpoint of exhibiting excellent anti-glycation effects when used in combination with other specific components.

[0023] In the present invention, various processed products of mugwort can be used, and the processed form can be selected from the same processed form as the raw material containing the above-mentioned polyamine. Among them, crushed product or crushed powder, squeezed product or squeezed powder, extract or extract powder are preferred, and crushed product or crushed powder is particularly preferred. In the present invention, it is most preferred to use crushed product or crushed powder of stems and leaves, since it shows excellent anti-glycation effect when used in combination with other specific components.

[0024] (Pomegranate) In the present invention, pomegranate belongs to the genus Punica in the family Lythraceae, and its scientific name is Punica granatum. The parts of pomegranate that can be used in the present invention include flowers, seeds, fruits, leaves, stems, and roots, but it is preferable to use flowers because they show excellent anti-glycation effects when used in combination with other specific components.

[0025] In the present invention, various processed pomegranate products can be used, and the processing form can be selected from the same processing form as the raw material containing the above-mentioned polyamines. Among them, crushed or crushed powder, squeezed or squeezed juice powder, extract or extract powder are preferred, extract or extract powder is more preferred, and hydrous ethanol extract or extract powder is particularly preferred. In the present invention, it is most preferred to use hydrous ethanol extract or hydrous ethanol extract powder of the flower, since it shows excellent anti-glycation effect when used in combination with other specific components.

[0026] (Rose) In the present invention, rose is a plant belonging to the genus Rosa and the family Rosaceae. The parts of rose that can be used in the present invention include flowers, seeds, fruits, leaves, stems, and roots, but it is preferable to use flowers because they show excellent anti-glycation effects when used in combination with other specific components.

[0027] In the present invention, various processed rose products can be used, and the processing form can be selected from the same processing form as the raw material containing the above-mentioned polyamine. Among them, crushed product or crushed powder, squeezed product or squeezed powder, extract or extract powder are preferred, extract or extract powder is more preferred, and water extract or extract powder is particularly preferred. In the present invention, it is most preferred to use water extract or water extract powder of flowers, since it shows excellent anti-glycation effect when used in combination with other specific components.

[0028] (Safflower) In the present invention, safflower is an annual or biennial plant of the genus Carthamus in the family Asteraceae, and its scientific name is Carthamus tinctorius. The parts of roses that can be used in the present invention include flowers, seeds, fruits, leaves, stems, and roots, but it is preferable to use flowers because they show excellent anti-glycation effects when used in combination with other specific components.

[0029] In the present invention, various processed safflower products can be used, and the processing form can be selected from the same processing form as the raw material containing the above-mentioned polyamine. Among them, crushed product or crushed powder, squeezed product or squeezed powder, extract or extract powder are preferred, extract or extract powder is more preferred, and water extract or extract powder is particularly preferred. In the present invention, it is most preferred to use the water extract or water extract powder of the flower, since it shows excellent anti-glycation effect when used in combination with other specific component materials.

[0030] The composition of the present invention can be used, for example, as so-called health foods such as pharmaceuticals, cosmetics (including quasi-drugs), functional foods such as foods for specified health uses, foods with nutrient functions, and foods with functional claims whose efficacy has been approved by a designated organization, as well as general foods, food additives, feed, etc.

[0031] Since the composition of the present invention has an anti-glycation effect, it can be used for the prevention and / or improvement of various symptoms caused by glycation, such as aging, alopecia / hair loss, diabetes, arteriosclerosis, osteoporosis, bone loss, infertility, Alzheimer's dementia, senile plaques in the brain, sexual dysfunction, decreased function of the eye's focusing muscles, as well as symptoms that may affect beauty, such as skin wrinkles, skin blemishes, sagging skin, dry skin, decreased skin elasticity, loss of skin transparency, yellowing of the whites of the eyes, and clouding of the iris of the eye. Therefore, the composition of the present invention can be used as an anti-glycation composition or a cosmetic composition. The anti-glycation composition and cosmetic composition are not particularly limited as long as they can be distinguished from other products as products in terms of their use for anti-glycation and their effects or efficacy against various symptoms caused by anti-glycation, including the above-mentioned symptoms that may affect beauty, and for example, the scope of the present invention includes not only those that indicate that they have anti-glycation or cosmetic functions on the main body, packaging, instructions, or promotional materials of the product according to the present invention, but also those that indicate that they have other functions, or those that have no indication of functions but actually have anti-glycation or cosmetic effects. Note that the anti-glycation composition and cosmetic composition of the present invention are not limited to those that indicate the combined materials of the present invention (polyamine, safflower, chrysanthemum, etc.) as anti-glycation or cosmetic active ingredients on the product packaging, etc. For example, the active ingredients may not be specified, or only some of the ingredients may be listed as active ingredients.

[0032] Specifically, examples of the anti-glycation composition and cosmetic composition of the present invention include medicines, so-called health foods, and cosmetics (including quasi-drugs). Examples of health foods that promote so-called anti-glycation and beauty include those that claim to have "prevention of aging," "anti-aging," "anti-aging," "prevention of glycation," "prevention of increase in AGEs," "inhibits activation of RAGE," "reduces the expression level of RAGE," "prevents internal burning," "for clear everyday life," "for those concerned with dull skin," "for those concerned with yellowish dullness," "for those concerned with sagging skin," "for those concerned with wrinkles," "for those concerned with blemishes," "for those with dry skin," "good for beauty," "good for the skin," "supports beauty," etc.

[0033] The composition of the present invention can be used for external or oral administration. External applications are not particularly limited as long as they are applied or attached to the skin, scalp, etc., and examples of the forms include external preparations for the skin such as ointments, creams, gels, lotions, emulsions, packs, and poultices, and injections.

[0034] Examples of oral forms include tablets, capsules, powders, granules, liquids, grains, rods, plates, blocks, solids, rounds, pastes, creams, caplets, gels, chewable tablets, sticks, etc. Among these, tablets, capsules, powders, granules, and liquids are preferred, and tablets, powders, and granules are particularly preferred. Specific examples include supplements, packaged beverages filled in PET bottles, cans, jars, etc., instant beverages to be dissolved in water (hot water), milk, fruit juice, green juice, etc., and food additives. In particular, supplements and instant beverages are preferred because they are easy to drink during meals and can enhance palatability.

[0035] The composition according to one embodiment of the present invention can be made into a containerized composition that is packed and sealed in a container. The container is not particularly limited, and examples thereof include packaging containers such as metals such as aluminum, paper, plastics such as PET and PTP, single-layer or laminated film bags, retort pouches, vacuum packs, aluminum containers, plastic containers, bottles, cans, etc. In order to prevent deterioration over time, the composition of one embodiment of the present invention is preferably sterilized by pressurization and / or heat after being packed and sealed in a container.

[0036] The method of using the composition of one embodiment of the present invention is not particularly limited, but for example, the composition of one embodiment of the present invention can be orally ingested as it is, together with water or the like, or after diluting with water or the like. Depending on the preference of the user, the composition of one embodiment of the present invention may be mixed with other solid or liquid materials and orally ingested. When the composition of one embodiment of the present invention is in an orally disintegrating dosage form, it can be orally ingested without water.

[0037] The content of the specific material in the composition of the present invention may be appropriately determined within the range in which the effect of the specific material is exhibited.

[0038] In general, when the composition of the present invention is a supplement or pharmaceutical in the form of a tablet or chewable tablet, the total amount of the materials of the present invention is preferably 0.1 to 80% of the total, more preferably 0.5 to 60%, and even more preferably 1 to 50%, calculated as dry mass.

[0039] When the composition of the present invention is a packaged beverage, the material of the present invention is preferably contained in a total amount of 0.0001 to 5% of the total, more preferably 0.0005 to 3%, and even more preferably 0.001 to 1%.

[0040] When the composition of the present invention is an instant beverage, the total amount of the ingredients of the present invention is preferably 0.01 to 50% of the total, calculated as dry mass, more preferably 0.05 to 30%, and even more preferably 0.1 to 20%.

[0041] Furthermore, when the composition of the present invention is for cosmetic use, the total amount of the materials of the present invention is preferably 0.0001 to 75% of the total, calculated as dry mass, more preferably 0.0005 to 50%, and even more preferably 0.001 to 25%.

[0042] The amount of intake of the composition of the present invention is not particularly limited, but from the viewpoint of more clearly exerting the effects of the present invention, it is preferable that the total amount of the material of the present invention is 0.001 mg / day or more per day for an adult, more preferably 0.01 mg / day or more, and even more preferably 0.1 mg / day or more. The upper limit is, for example, 100000 mg / day, preferably 50000 mg / day. The oral composition of the present invention can be stored as a daily amount in one container or divided into, for example, 2 to 3 multiple containers so that the daily intake is the above-mentioned amount.

[0043] The blending ratio of each material in the composition of the present invention is not particularly limited, but for example, the blending mass ratio of the polyamine or a salt thereof and other materials is preferably in the range of 1:1 to 50,000, more preferably in the range of 1:3 to 30,000, even more preferably in the range of 1:5 to 10,000, and particularly preferably in the range of 1:10 to 5,000, calculated on a dry mass basis.

[0044] The blending mass ratio of chrysanthemum to other materials, calculated on a dry mass basis, is preferably in the range of 1:100 to 50,000:1, more preferably in the range of 1:0.0001 to 10,000, even more preferably in the range of 1:0.001 to 1,000, and particularly preferably in the range of 1:0.01 to 100.

[0045] The blending mass ratio of polyamine or a salt thereof, chrysanthemum and safflower, calculated on a dry mass basis, is preferably in the range of 1:1-30,000:1-30,000, more preferably in the range of 1:3-10,000:3-10,000, even more preferably in the range of 1:5-7,500:5-7,500, and particularly preferably in the range of 1:10-5,000:10-5,000.

[0046] The composition of the present invention can be produced by a known method by adding other components other than the components of the present invention as necessary. Examples of other components other than the components of the present invention include vitamins such as water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P) and oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, and iron; sulfur-containing compounds contained in taurine and garlic; flavanoids or flavonoids such as hesperidin and quercetin; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; crushed products or extracts of animals and plants, etc. EXAMPLES

[0047] The present invention will be specifically described below based on examples.

[0048] [Evaluation of anti-glycation effect of the composition] The following substances were used as test substances. As the polyamine, rice germ citric acid aqueous solution extract powder (commercial product; polyamine content 0.2% or more) was used. For the chrysanthemum, crushed purple chrysanthemum flowers (commercially available) were used. As safflower, a water extract of safflower flowers (commercially available product) was used. As the rose, a water extract powder of rose flowers (commercially available product) was used. For pomegranate, we used a hydrous ethanol extract powder of pomegranate flowers (commercially available product). - As barley, ground barley leaves (manufactured by Toyo Shinyaku Co., Ltd.) were used. -Mugwort powder (manufactured by Toyo Shinyaku Co., Ltd.) was used as the mugwort.

[0049] Phosphate buffer powder (1 / 15 mol / L pH 7.2) was dissolved in distilled water to prepare 67 mM phosphate buffer (hereinafter abbreviated as 67 mM PB). D(+) glucose was dissolved in 67 mM PB to prepare a 200 mg / mL glucose solution, and albumin (bovine serum-derived Cohn fraction V, pH 7.0, for biochemistry) (hereinafter abbreviated as BSA) was dissolved in 67 mM PB to prepare a 40 mg / mL BSA solution.

[0050] A given amount of each of the test substances in Comparative Examples 1 to 17 and Examples 1 to 10 was vortexed in 67 mM PB for 1 hour, centrifuged (room temperature, 10,000 rpm, 5 minutes), and the supernatant was collected to prepare a test substance solution. The test substance solution, glucose solution, BSA solution, and PB were mixed in a 1.5 mL tube in the amounts shown in Table 1 to prepare a solution (test solution) containing the test substance to be used in this test. Similarly, a control not containing the test substance was prepared. The concentrations of the test solutions in each of the Comparative Examples and Examples relating to the combination of polyamine and chrysanthemum, safflower, barley, or mugwort are shown in Tables 2 to 5, and the concentrations of the test solutions in each of the Comparative Examples and Examples relating to the combination of chrysanthemum and safflower, rose, or pomegranate are shown in Tables 6 to 8. The unit of values ​​in Tables 2 to 8 is mg / mL.

[0051] The test solution or control solution prepared in a 1.5 mL tube was mixed by inversion and then incubated at 60°C for 48 hours. After incubation, 30 μL of each solution was transferred to a 96-well plate and diluted with 270 μL of pure water. The fluorescence intensity at 440 nm when the diluted solution was excited at 370 nm was measured using a spectrofluorophotometer, and the AGE formation inhibition rate (%) was calculated using the following formula.

[0052] AGEs production inhibition rate (%) =(1 - [(Sample test -Sample blank ) / (Control test -Control blank ) ]) × 100 (In the formula, Sample test : Fluorescence intensity of test solution (test) Sample blank : Fluorescence intensity of test solution (blank) Control test : Fluorescence intensity of control (test) Control blank : Fluorescence intensity of control (blank) The results are shown in Figures 1 to 7.

[0053] [Table 1]

[0054] [Table 2]

[0055] [Table 3]

[0056] [Table 4]

[0057] [Table 5]

[0058] [Table 6]

[0059] [Table 7]

[0060] [Table 8]

[0061] As is clear from Figures 1 to 4, in Examples 1 to 6 in which polyamine or its salt was combined with chrysanthemum, safflower, barley, or mugwort, a dramatic effect was observed that could not be obtained by adding up the effects of each material alone. In addition, as is clear from Figures 5 to 7, in Examples 8 to 10 in which chrysanthemum was combined with safflower, rose, or pomegranate, a dramatic effect was observed that could not be obtained by adding up the effects of each material alone. Therefore, it can be seen that the composition of the present invention has an excellent anti-glycation effect. Furthermore, from Figure 4, in Example 7 in which three materials, polyamine or its salt, chrysanthemum, and safflower, were combined, a dramatic effect was observed that could not be obtained by adding up the effects of each material alone, and the effect was more remarkable than that of the combination of two materials selected from polyamine or its salt, chrysanthemum, and safflower in Examples 1, 2, 6, and 10. Therefore, it can be seen that the composition containing three types of polyamine or its salt, chrysanthemum, and safflower has a particularly excellent anti-glycation effect compared to the combination of two materials selected from polyamine or its salt, chrysanthemum, and safflower.

[0062] [Production of the composition of the present invention] [Example 11] (Preparation of tablets) Tablets (300 mg) made from the following materials were manufactured. Two tablets were taken per day. The manufactured tablets were taken once or twice a day with water to obtain an excellent anti-glycation effect. Furthermore, HPLC analysis showed that the obtained tablets contained 0.01% polyamine or its salt, and the proportion of spermine in the polyamine or its salt was 50%.

[0063] Rice germ citric acid aqueous solution extract powder 2% Purple chrysanthemum flower powder 10% Safflower flower water extract powder 10% Rose flower hot water extract powder 10% Pomegranate flower hydroethanolic extract powder 10% Vitamin C 2% Gum arabic 1% Calcium stearate 1% Silicon dioxide 1% maltose remainder

[0064] [Example 12] (Preparation of tablets) Tablets (350 mg) made from the following ingredients were manufactured. Six tablets were taken per day. The manufactured tablets can be taken once a day or two or three times a day with water to obtain excellent anti-glycation effects.

[0065] Rice germ citric acid aqueous solution extract powder 5% Purple chrysanthemum flower powder 10% Barley stem and leaf powder 10% Mugwort stem and leaf powder 10% Lactic acid bacteria 5% Calcium stearate 1% Reduced maltose remainder

[0066] [Example 13] (Production of capsules) The mixture below was packed into a hard capsule to produce a capsule formulation (300 mg). Five of the resulting hard capsules were taken per day. The capsule formulation thus produced can be taken once a day or 2 to 4 times a day with water to obtain an excellent anti-glycation effect.

[0067] Rice germ acetic acid extract powder 30% Safflower flower powder 5% Barley stem and leaf juice powder 20% Mugwort stem and leaf powder 15% Lactic acid bacteria 5% Vitamin C 1% Folic Acid 1% Silicon dioxide 2% Cellulose Remainder

[0068] [Example 14] (Preparation of granules) The following materials were mixed and prepared into granules (3000 mg) by a conventional method. The obtained granules have an excellent anti-glycation effect when taken with water. The obtained granules contained 0.01% polyamine or its salt.

[0069] Rice germ acetic acid extract powder 3% Purple chrysanthemum flower water extract powder 5% Pomegranate juice powder 10% Rose flower hot water extract powder 5% Mugwort leaf juice powder 2% Aspartame 0.1% Thiamine hydrochloride 0.3% Riboflavin 0.3% Vitamin B6 0.2% Cyanocobalamin 0.2% Fragrance 0.2% Reduced Palatinose 7% Calcium stearate 3% Hydroxypropyl cellulose Remainder

[0070] [Example 15] (Preparation of chewable tablets) Chewable tablets (1000 mg per tablet) were manufactured from the following ingredients. Six of the obtained chewable tablets were taken per day. The manufactured chewable tablets can be taken once a day or two or three times a day with water to obtain excellent anti-glycation effects.

[0071] Soybean germ extract powder with citric acid solution 5% Purple chrysanthemum flower hot water extract 3% Hot water extract of rose flowers 10% Safflower flower water extract powder 5% Barley stem and leaf juice powder 5% Sucralose 0.1% Aspartame 0.05% Vitamin B1 0.01% Vitamin B2 0.01% Vitamin B6 0.01% Fragrance 0.01% Calcium stearate 1% Silicon dioxide 2% Hydroxypropyl cellulose 1% Cellulose 30% Reduced Palatinose Remainder

[0072] [Example 16] (Production of cosmetic cream) A cosmetic cream was produced from the following ingredients. The obtained cosmetic cream provides excellent anti-glycation effects when applied.

[0073] Rice germ citric acid aqueous solution extract powder 0.1% Purple chrysanthemum flower powder 0.1% Safflower flower water extract powder 0.1% 1,3-butylene glycol extract powder from mugwort stems and leaves 0.1% Squalane 15% Octyldodecyl myristate 4% Hydrogenated soy phospholipids 0.2% Butyl alcohol 2% Hydrogenated oil 2% Stearic acid 2% Lipophilic glyceryl monostearate 2% Polyglyceryl monostearate 0.5% Behenyl alcohol 0.8% Polyglyceryl monomyristate 0.7% White beeswax 0.3% d-δ-tocopherol 0.1% Methylparaben 0.3% C10-30 alkyl modified carboxyvinyl polymer 0.2% Carboxyvinyl polymer 0.1% 1,3-Butanediol 18% Sodium hydroxide 0.1% Purified water remainder [Industrial Applicability]

[0074] Since the composition of the present invention has anti-glycation activity and the like, the present invention is highly industrially useful.

Claims

[Claim 1] The composition contains pomegranate and at least one selected from chrysanthemum, rice germ extract powder, mugwort, safflower, rose and barley.

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