Cosmetic antibacterial composition and cosmetic

Abstract

To provide a composition suitable for cosmetic applications, formulated from waste mushroom beds.SOLUTION: A cosmetic antibacterial composition contains at least one selected from the group consisting of Lentinus edodes waste mushroom bed extract, Flammulina filiformis waste mushroom bed extract, Hypsizygus marmoreus waste mushroom bed extract, and Grifola frondosa waste mushroom bed extract.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an antibacterial composition for cosmetics and a cosmetic. [Background technology]

[0002] Active ingredients produced by fungi, such as mushrooms, have attracted attention for their various functional components, such as anticancer and antibacterial properties, which are effective for humans, animals, and plants. Methods for extracting the active ingredients from mushrooms and the effects of these active ingredients are being researched. In particular, antibacterial substances produced by mushrooms are being considered for use as antibiotics, pesticides, medicines, etc. (Patent Document 1), and their possible use in cosmetic compositions has also been suggested (Patent Document 2). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent No. 5561637 [Patent Document 2] Patent Publication No. 2021-123550 Summary of the Invention [Problem to be solved by the invention]

[0004] Mushrooms are cultivated on mushroom beds. A mushroom bed is a medium made by mixing nutrients such as rice bran with sawdust and solidifying it, and the mushrooms are cultivated by inoculating the seed fungus into the medium. After mushroom cultivation, a large amount of waste mushroom beds is generated and must be disposed of as industrial waste, so there has been a desire to utilize the waste mushroom beds for other purposes.

[0005] In view of the above circumstances, the present invention aims to provide a composition made from waste mushroom bed as a raw material and usable for cosmetic purposes. [Means for solving the problem]

[0006] As a result of extensive investigations aimed at solving the above problems, the present inventors arrived at the following invention. In other words, the present invention relates to an antibacterial composition for cosmetics, characterized by containing at least one selected from the group consisting of a shiitake mushroom waste mushroom bed extract, a Enokitake mushroom waste mushroom bed extract, a Bunashimeji mushroom waste mushroom bed extract, and a Maitake mushroom waste mushroom bed extract, and a cosmetic comprising the above-mentioned antibacterial composition for cosmetics. Effect of the Invention

[0007] According to the present invention, a composition can be provided that is made from waste mushroom bed as a raw material and can be used for cosmetic purposes. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] <Antibacterial composition for cosmetics> The antibacterial composition for cosmetics of the present invention is characterized by containing at least one selected from the group consisting of a shiitake mushroom waste mushroom bed extract, a enokitake mushroom waste mushroom bed extract, a Bunashimeji mushroom waste mushroom bed extract, and a Maitake mushroom waste mushroom bed extract.

[0009] In this specification, the antibacterial composition for cosmetics means an antibacterial composition for cosmetics that can be used as a cosmetic raw material. In addition, the term "cosmetics" in this specification refers to cleansing agents for washing hair or skin (shampoo, pump foamer cleanser, cream cleanser, facial cleanser, body soap, solid soap, cleansing agent, liquid soap, etc.), hair care cosmetics (hair rinse, conditioner, non-cationic conditioner, treatment, hair oil, hair styling agent, etc.), skin care cosmetics (lotion, face cream, hand cream, cleansing oil, skin lotion, milky lotion, all-in-one gel, shaving agent, etc.), makeup cosmetics (foundation, makeup base, powder, lipstick, blusher, eyeliner, eye shadow, eyebrow, mascara, etc.), hair cosmetics (hair wax, hair gel, hair spray, hair coloring agent, etc.), UV care cosmetics (sunscreen, cream type, gel type, roll-on type, etc.), wipe-off cosmetics (mask, makeup remover sheet, sweat wipe sheet, hair wipe sheet, etc.), fragrance products, antiperspirants, etc. In this specification, the names of compounds may be described by their display names or alternate display names listed in the "List of Cosmetic Ingredient Display Names" prepared by the Japan Cosmetic Industry Association.

[0010] In this specification, the term "waste mushroom bed" refers to a mushroom bed used for cultivating mushrooms. The term "waste mushroom bed extract" refers to an extract obtained by extracting the waste mushroom bed with a solvent. The mushroom bed is a medium made by mixing nutrients such as rice bran with sawdust and solidifying it, and the components derived from the mushroom mycelium come to be contained in the mushroom bed as a result of the cultivation of mushrooms. The mushroom bed whose composition has changed from that of the previous bed due to the inclusion of components derived from the mycelium as a result of the cultivation of mushrooms is referred to as a waste mushroom bed in this specification. That is, the mushroom bed is removed during the cultivation of mushrooms, and the extract obtained from the removed mushroom bed is designated as a waste mushroom bed extract. Naturally, the mushroom bed that becomes waste after the cultivation of mushrooms is completed is also referred to as the waste mushroom bed in this specification, and the extract of the waste mushroom bed is the waste mushroom bed extract.

[0011] Methods for obtaining a waste mushroom bed extract include extraction with a solvent, extraction with supercritical carbon dioxide, supercritical water, or subcritical water, and dry distillation. The solvent used for extraction may be water or an organic solvent, such as ethanol, 1,3-butylene glycol, or ethyl acetate, with ethyl acetate being preferred.

[0012] The waste mushroom bed extract may be at least one selected from the group consisting of a shiitake mushroom waste mushroom bed extract, a Enokitake mushroom waste mushroom bed extract, a Bunashimeji mushroom waste mushroom bed extract, and a Maitake mushroom waste mushroom bed extract. Among these, extracts from spent shiitake mushroom beds are preferred. The waste mushroom bed extract may contain ketones, polysaccharides, organic acids, fatty acids, and the like, as components that are thought to exhibit antibacterial properties. Ketones include 3-octanone. Polysaccharides include chitosan. Organic acids include malic acid, citric acid, succinic acid, fumaric acid, pyroglutamic acid, oxalic acid, lactic acid, tartaric acid, gluconic acid, etc. Fatty acids include linoleic acid, palmitic acid, stearic acid, oleic acid, pentadecanoic acid, linolenic acid, palmitoleic acid, lignoceric acid, etc.

[0013] The target bacteria against which the antibacterial composition for cosmetics exerts antibacterial properties is preferably at least one species selected from the group consisting of gram-negative bacteria, gram-positive bacteria, and fungi.

[0014] Examples of gram-negative bacteria include bacteria of the genera Escherichia, Thermus, Rhizobium, Pseudomonas, Shewanella, Vibrio, Salmonella, Acetobacter, and Synechocystis. The antibacterial cosmetic composition of the present invention is particularly effective against Escherichia coli and Pseudomonas aeruginosa.

[0015] Examples of Gram-positive bacteria include the genera Bacillus, Saccharomyces, Candida, Corynebacterium, Brevibacillus, Bifidobacterium, Lactococcus, Enterococcus, Pediococcus, Leuconostoc, and Streptomyces. The antibacterial cosmetic composition of the present invention is particularly effective against Staphylococcus aureus and Candida albicans.

[0016] Examples of fungi include Basidiomycota, Ascomycota, Chytridiomycota, Neocallimastigomycota, Blastocladiomycota, Microsporidia, Glomeromycota, Mucoromycotina, Entomophthoromycotina, Zoopagomycotina, and Kickxellomycotina. The antibacterial composition for cosmetics of the present invention is particularly effective against Aspergillus niger. There is a demand for an antibacterial composition for cosmetics that is highly effective against Aspergillus niger, and the antibacterial composition for cosmetics of the present invention is particularly effective as a composition that can exhibit antibacterial properties against Aspergillus niger. In addition, an antibacterial composition for cosmetics containing an extract from a waste mushroom bed is particularly effective as an antibacterial composition for cosmetics that can exhibit antibacterial activity against Aspergillus niger.

[0017] The antibacterial composition for cosmetics of the present invention may contain the solid content contained in the waste mushroom bed extract, and a dried product (solid or viscous product) obtained by removing the solvent from the waste mushroom bed extract is also an antibacterial composition for cosmetics. In addition, a product obtained by concentrating or diluting the waste mushroom bed extract to change the concentration of the solid content contained in the waste mushroom bed extract is also an antibacterial composition for cosmetics. The solid content contained in the waste mushroom bed extract means the components obtained by removing the solvent from the waste mushroom bed extract, and may be in the form of a solid or a viscous liquid.

[0018] Furthermore, the antibacterial composition for cosmetics of the present invention may be a waste mushroom bed extract itself, containing only a waste mushroom bed extract, or may be an antibacterial agent for cosmetics consisting of a waste mushroom bed extract. The antibacterial cosmetic composition of the present invention may contain other components in addition to the waste mushroom bed extract, and may contain additives such as antioxidants and ultraviolet absorbers that are added for storage as an antibacterial cosmetic composition before preparation of the cosmetic. Also, it may contain a solvent other than the solvent used for extraction of the waste mushroom bed extract.

[0019] <Cosmetics> The cosmetic of the present invention contains the antibacterial composition for cosmetics of the present invention. Examples of the cosmetic include a detergent for washing hair or skin, a hair care cosmetic, a skin care cosmetic, a makeup cosmetic, a hair cosmetic, a UV care cosmetic, a wipe-off cosmetic, a fragrance product, and an antiperspirant.

[0020] The weight ratio of the antibacterial composition for cosmetics of the present invention in the cosmetic of the present invention can be appropriately adjusted depending on the formulation and use of the cosmetic. The weight ratio of the antibacterial composition for cosmetics, calculated as the solid content of the waste mushroom bed extract contained in the antibacterial composition for cosmetics (components obtained by removing the solvent from the waste mushroom bed extract), is, for example, preferably 0.1 to 10% by weight when the cosmetic is a cleanser, preferably 0.1 to 10% by weight when the cosmetic is a hair care cosmetic, preferably 0.1 to 10% by weight when the cosmetic is a skin care cosmetic, preferably 0.1 to 10% by weight when the cosmetic is a makeup cosmetic, preferably 0.1 to 10% by weight when the cosmetic is a hair cosmetic, preferably 0.1 to 10% by weight when the cosmetic is a UV care cosmetic, preferably 0.1 to 10% by weight when the cosmetic is a wipe-off cosmetic, and preferably 0.1 to 10% by weight when the cosmetic is a fragrance product or antiperspirant. Within this range, the cosmetic composition has good antibacterial properties and can be prevented from spoiling, and the cosmetic composition can be made gentle on the skin.

[0021] The cosmetic of the present invention may contain other components in addition to the antibacterial cosmetic composition. Examples of other components include known cosmetic raw material components used as cosmetic raw materials, such as anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants, water, pH adjusters, solvents, conditioning agents, oils, cooling agents, moisturizers, antioxidants, chelating agents, thickeners, fragrances, colorants, preservatives, ultraviolet protection agents, and skin whitening agents.

[0022] Examples of the anionic surfactant include ether carboxylic acids or their salts, sulfate salts, sulfonate salts, phosphate salts, fatty acid salts, and acylated amino acid salts. Specific examples include sodium laureth-4 carboxylate, sodium lauryl glycol carboxylate, stearic acid, and isostearic acid.

[0023] Cationic surfactants include quaternary ammonium salts and amine salts. Specific examples include behentrimonium chloride.

[0024] Examples of nonionic surfactants include polypropylene glycol (PPG-34, etc.), alkylene oxide (having 2 to 8 carbon atoms) adducts of alcohols having 4 to 24 carbon atoms, esters or ethers of fatty acids having 8 to 24 carbon atoms and alcohols or alkylene oxide (having 2 to 8 carbon atoms) polymers, alkylene oxide adducts of higher fatty acid esters of polyhydric (dihydric to decahydric) alcohols, polyglycerin fatty acid esters, and fatty acid alkanolamides. Specific examples include cocamide DEA (coconut oil fatty acid diethanolamide), PEG-75-PEG-300-hexylene glycol, PEG-60 hydrogenated castor oil, PEG-23 stearate, polyglyceryl-10 isostearate, glyceryl stearate, PEG-190 distearate, PPG-7 buteth-10, laureth-7 (polyoxyethylene (degree of polymerization 7) lauryl ether) and ceteth-20 (polyoxyethylene (degree of polymerization 20) cetyl ether).

[0025] Examples of the amphoteric surfactant include alkylamino fatty acid salts, alkyldimethylacetate betaines, fatty acid amidopropyl betaines, alkylimidazolinium betaines, and sulfobetaine-type amphoteric surfactants. Specific examples include sodium cocoamphoacetate and cocamidopropyl betaine.

[0026] Examples of water include ordinary water, purified water, pure water, ultrapure water, as well as hard water, soft water, natural water, deep sea water, hot spring water, electrolytic alkaline ionized water, electrolytic acidic ionized water, ion-exchanged water, and cluster water.

[0027] Examples of pH adjusters include lactic acid, citric acid, phosphoric acid, malic acid, tartaric acid, hydrochloric acid, acetic acid, sodium hydroxide, potassium hydroxide, triethanolamine, amino acids (such as arginine), and salts thereof.

[0028] Solvents include ethanol, isoprene diol, denatured alcohol, dipropylene glycol (DPG), 1,2-hexanediol, isododecane, isopropanol, isopentyl diol, butyl acetate, ethoxydiglycol, and propylene glycol.

[0029] Conditioning agents include polyquaternium-10 (a polymer of a quaternary ammonium salt obtained by adding glycidyltrimethylammonium chloride to hydroxyethylcellulose), polyquaternium-7 (a polymer of a quaternary ammonium salt obtained from acrylic acid amide and dimethyldiallylammonium chloride), polyquaternium-22 (a copolymer of dimethyldiallylammonium chloride and acrylic acid), polyquaternium-56 (a polymer of a quaternary ammonium salt consisting of isophorone diisocyanate, butylene glycol and methyl sulfate of dihydroxyethyldimonium), (VP / VA) copolymer (a copolymer of vinyl acetate and vinylpyrrolidone), guar hydroxypropyltrimonium chloride (a quaternary ammonium salt obtained by adding glycidyltrimethylammonium chloride to guar gum), PEG-34, PEG-400, sodium polyacrylate, hydroxyethylcellulose, and panthenol.

[0030] Examples of the oil agent include liquid oils, solid oils, hydrocarbon oils, synthetic ester oils, silicone oils, and essential oils. Specific examples include triethylhexanoin, squalane, dimethicone, dipentaerythrityl hexa(hydroxystearic acid / stearic acid / rosin acid) (hexaester of hydroxystearic acid, stearic acid, and rosin acid with dipentaerythritol), hydrogenated palm oil, mineral oil, candelilla wax, beeswax, ethylhexyl palmitate, and shea butter.

[0031] Cooling agents include menthol, peppermint oil, thymol, methyl salicylate, and camphor.

[0032] Examples of moisturizing agents include glycerin, BG (1,3-butylene glycol), pentylene glycol, hydrogenated rapeseed oil alcohol, sorbitol, sodium lactate, PCN-Na (sodium pyrrolidone carboxylate), sodium hyaluronate, and sodium chondroitin sulfate.

[0033] Antioxidants include vitamin E (tocopherol), BHT (dibutylhydroxytoluene), BHA (butylhydroxyanisole), dipotassium glycyrrhizinate, ascorbyl palmitate, and rosemary leaf extract.

[0034] Examples of the chelating agent include EDTA (ethylenediaminetetraacetic acid), EDTA-2Na (disodium salt of ethylenediaminetetraacetic acid), sodium polyphosphate, disodium pyrophosphate, gluconic acid, sodium gluconate, and ascorbic acid.

[0035] Examples of thickening agents include guar gum, xanthan gum, starch, behenyl alcohol, stearyl alcohol, cetearyl alcohol, cetyl alcohol, myristyl alcohol, carbomer, hydroxypropyl methylcellulose, polyvinyl alcohol, sodium polyacrylate, sodium acrylate grafted starch, disteardimonium hectorite, talc, glycol distearate, and corn starch.

[0036] Fragrances include limonene, β-caryophyllene, linalool, farnesol, phenethyl alcohol, citral, hexyl cinnamal, ionone, linalyl acetate, benzyl benzoate, undecalactone, cinnamaldehyde, anise oil, and jasmine oil.

[0037] Coloring agents include Blue No. 1, Blue No. 2, Green No. 3, and Red No. 1.

[0038] Preservatives include phenoxyethanol, o-cymen-5-ol, methylparaben, ethylparaben, propylparaben, isopropylparaben, butylparaben, isobutylparaben, and isopropylmethylphenol.

[0039] Examples of ultraviolet protection agents include titanium oxide, zinc oxide, ethylhexyl methoxycinnamate, ethylhexyl dimethyl PABA, diethylamino hydroxybenzoyl hexyl benzoate, and t-butyl methoxydibenzoylmethane.

[0040] Skin whitening agents include tranexamic acid, arbutin, and hydroquinone.

[0041] The content of other ingredients in the cosmetic of the present invention can be appropriately adjusted depending on the formulation and use of the cosmetic. For example, the solvent contained in the cosmetic is preferably 95% by weight or less based on the total weight of the cosmetic, the anionic surfactant, cationic surfactant, nonionic surfactant, amphoteric surfactant, oil agent and moisturizing agent contained in the cosmetic are each preferably 50% by weight or less based on the total weight of the cosmetic, the whitening agent and conditioning agent are each preferably 20% by weight or less based on the total weight of the cosmetic, the pH adjuster, cooling agent, antioxidant, chelating agent, thickener, UV protection agent, fragrance and colorant are each preferably 10% by weight or less based on the total weight of the cosmetic, the water contained in the cosmetic is preferably 95% by weight or less based on the total weight of the cosmetic, and the preservative contained in the cosmetic is preferably 0.5% by weight or less based on the total weight of the cosmetic. In this specification, when the anionic surfactant contains a fatty acid salt, it is assumed that all the fatty acids in the cosmetic are potassium salts, and the weight of the potassium salt of the fatty acid is regarded as the weight of the fatty acid salt in the cosmetic.

[0042] The present specification discloses the following:

[0043] The present disclosure (1) is an antibacterial composition for cosmetics, characterized by containing at least one selected from the group consisting of a shiitake mushroom waste mushroom bed extract, a enokitake mushroom waste mushroom bed extract, a Bunashimeji mushroom waste mushroom bed extract, and a Maitake mushroom waste mushroom bed extract.

[0044] The present disclosure (2) is an antibacterial composition for cosmetics according to (1), wherein the target bacteria is at least one species selected from the group consisting of gram-negative bacteria, gram-positive bacteria, and fungi.

[0045] The present disclosure (3) is a cosmetic comprising the antibacterial composition for cosmetics according to (1) or (2). EXAMPLES

[0046] The present invention will be further described below with reference to examples and comparative examples, but the present invention is not limited to these. <Preparation of Shiitake Mushroom Waste Bed Extract> 100 g of spent shiitake mushroom beds were added to 500 g of boiling distilled water and boiled for 10 minutes to obtain a broth, which was then filtered through double gauze and then suction filtered. The filtrate was partitioned and extracted twice with an equal volume of ethyl acetate, and the ethyl acetate layer was concentrated using an evaporator to prepare a Lentinula edodes waste bed extract.

[0047] <Antibacterial test> (Test strain) The test strains used in the test are as follows: Escherichia coli (NRBC3972 strain, National Institute of Technology and Evaluation) Pseudomonas aeruginosa (NRBC13275 strain, National Institute of Technology and Evaluation) Staphylococcus aureus (NRBC13276 strain, National Institute of Technology and Evaluation) Candida (Candida albicans: NRBC1594 strain, National Institute of Technology and Evaluation) Aspergillus brasiliensis (NRBC9455 strain, National Institute of Technology and Evaluation)

[0048] (Preparation of test bacteria solution) Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus Each test bacterium was pre-cultured on an SCD agar slant medium at 35°C for 20 hours. One loopful of the bacterium was transferred to Mueller-Hinton broth and pre-cultured at 35°C for 20 hours. The pre-culture solution was diluted with Mueller-Hinton broth to obtain approximately 10% 7 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0049] Candida The test bacteria were pre-cultured on potato dextrose agar medium at 25°C for 48 hours, then scraped off with a platinum loop and suspended in sterile saline. 7 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0050] Aspergillus niger The test bacteria was cultured on a potato dextrose agar slant medium at 25°C for 7 to 14 days. Sterile saline containing 0.05% polysorbate 80 was poured onto the slant, and the spores were scraped off with a platinum loop. This liquid was then filtered through a piece of sterile gauze folded in four, and then diluted with sterile saline containing 0.05% polysorbate 80, and approximately 10 6 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0051] (Preparation of antibacterial composition dilution) 1 g of the extract from the waste mushroom bed was placed in a 10 mL measuring flask and sterilized purified water was added to make a total volume of 10 mL to prepare a mother liquid (10% solution). The mother liquid was diluted with sterilized purified water to prepare serial dilutions of 100,000 to 200 μg / mL.

[0052] (Preparation of agar medium containing antibacterial composition) 9 mL of the agar medium was dispensed into test tubes, sterilized in an autoclave, and then kept at 50°C in a molten state. To this was added 1 mL of the diluted antibacterial composition, which was then thoroughly mixed using a vortex mixer and poured into a 60 mm petri dish to solidify into a flat plate. Mueller-Hinton agar medium was used for Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. For Candida and Aspergillus niger, potato dextrose agar medium was used.

[0053] (Inoculation and cultivation of test bacteria) Each test bacterial solution was collected using a disposable loop (1 mm) and streaked approximately 1 cm long onto an agar medium containing the antibacterial composition ("streaked" means inoculating the bacteria as if drawing a line on the agar medium), and then cultured at the specified temperature and time. Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus were cultured on Mueller-Hinton agar medium at 35°C for 48 hours. Candida and Aspergillus niger were cultured on potato dextrose agar medium at 25°C for 5 hours.

[0054] (judgement) The test was carried out using media with different concentrations of the diluted antibacterial composition, and the minimum concentration (MIC: minimum inhibitory concentration) of the extract from the waste mushroom bed in the diluted antibacterial composition at which the growth of the test bacteria was completely inhibited was determined. The results are shown in Table 1. [Table 1]

[0055] As shown in Table 1, when the extract from spent shiitake mushroom bed was used, the MIC value was low.

[0056] <Examples of cosmetic preparation> The following shows an example of preparing a cosmetic containing the antibacterial composition for cosmetics. The blending amount of the shiitake mushroom waste mushroom bed extract in each cosmetic composition example is shown as the blending amount of the solid content remaining after removing the solvent from the waste mushroom bed extract. (1) Facial cleanser An example of the composition of the facial cleanser is shown in the table below. [Table 2] Method for preparing face wash Heat components A and B to 40-50℃ to dissolve the ingredients. ·While stirring component A, gradually add component B. After stirring evenly, cool and degas.

[0057] (2) Lotion An example of the composition of the lotion is as shown in the table below. [Table 3] Method for preparing lotion Stir component A to dissolve the contents. Mix component B to disperse the ingredients. Add component B to component A and dissolve evenly. While stirring the mixture of components A and B, gradually add component C. After stirring evenly, degas the mixture.

[0058] (3) Face cream An example of the composition of the face cream is shown in the table below. [Table 4] How to prepare face cream Heat component B to 75-80℃ to dissolve the contents. Heat component A to 75-80℃ and gradually add component B while stirring component A. After stirring evenly, cool and degas.

[0059] (4) Shampoo An example of a shampoo composition is shown in the table below. [Table 5] Method of preparing shampoo Dissolve the citric acid in some of the water. Mix component B and heat to 80℃ to dissolve. Add component B to water heated to 80℃ while stirring, then add the ingredients of component A in the following order: water, citric acid, cocamidopropyl betaine, sodium laureth-4 carboxylate, and shiitake mushroom waste bed extract. After stirring evenly, cool and degas.

[0060] (5) Conditioner An example of the conditioner composition is shown in the table below. [Table 6] Method for Preparing the Conditioner Heat component A to 80-85℃. While stirring component A, gradually add component B and mix evenly. · Gradually add component C to the mixture of components A and B and stir evenly. Cool and degas.

[0061] (6) Hair styling products Examples of the composition of hair styling products are as shown in the table below. [Table 7] Method for preparing a hair styling product Heat component A to 80-85℃. While stirring component A, gradually add component B and mix evenly. · Gradually add component C to the mixture of components A and B and stir evenly. Cool and degas.

[0062] (7) Hand cream An example of the composition of the hand cream is shown in the table below. [Table 8] Method for preparing hand cream Heat component A to 80-85℃. · Add component B to component C and allow to swell. Heat the mixture of components B and C to 80-85℃. While stirring component A, gradually add the mixture of components B and C to emulsify. After stirring evenly, cool and degas.

[0063] (8) Sunscreen Examples of sunscreen compositions are shown in the table below. [Table 9] Method of preparing sunscreen Heat components A and B to 80-85℃. While stirring component A, gradually add component B and mix evenly. While stirring component C, add the mixture of components A and B and mix. While stirring the mixture of components A, B and C, add component D and mix. While stirring the mixture of components A, B, C and D, add component E and mix. Cool and degas.

[0064] <Antibacterial test using cosmetics> (Preparation of test bacteria solution) Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus Each test bacterium was pre-cultured on an SCD agar slant medium at 35°C for 20 hours. One loopful of the bacteria was suspended in sterile saline and incubated for approximately 10 min. 8 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0065] Candida The test bacteria was pre-cultured on Sabouraud glucose agar medium at 22.5℃ for 48 hours. One loopful of the bacteria was suspended in sterile saline and diluted with about 10 8 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0066] Aspergillus niger The test bacteria was pre-cultured on Sabouraud glucose agar medium at 22.5℃ for 6-10 days. One loopful of the bacteria was suspended in sterile saline containing 0.05% polysorbate 80, and approximately 10 8 The test bacteria solution was prepared to have a concentration of bacteria / mL.

[0067] (Inoculation and storage of test bacteria) For each type of test bacteria, 20 g of cosmetic material was placed in a sterile vial and 0.15 mL of the test bacteria liquid was inoculated. Each was stored at 22.5°C, and the viable cell count was measured on the 7th, 14th, 21st, and 28th days.

[0068] (Measurement of viable bacteria count) The viable counts were determined by the SCDLP agar pour plate method for bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) and by the Sabouraud-dextrose LP agar pour plate method for fungi (yeast and mold). The ratio of live bacteria to the inoculated bacteria was judged as ◎ if less than 5%, as ○ if 5% or more but less than 20%, as △ if 20% or more but less than 50%, and as × if 50% or more. The results are shown in Table 10. [Table 10]

[0069] As shown in Table 10, it was confirmed that the cosmetic preparation containing the antibacterial composition for cosmetics of the present invention, which contains an extract from a waste shiitake mushroom bed, has antibacterial properties against bacteria and fungi. [Industrial Applicability]

[0070] The antibacterial composition for cosmetics of the present invention is preferable from the viewpoint of sustainability because it is made from waste mushroom beds that are generated in large quantities during mushroom cultivation, and since it exhibits high antibacterial properties against bacteria and fungi, it can be used as an antibacterial composition for cosmetics to be used in cosmetics such as facial cleansers, lotions, face creams, shampoos, conditioners, hair styling agents, hand creams and sunscreens.

Claims

1. An antibacterial composition for cosmetics, comprising at least one selected from the group consisting of a shiitake mushroom waste mushroom bed extract, a enokitake mushroom waste mushroom bed extract, a Bunashimeji mushroom waste mushroom bed extract, and a Maitake mushroom waste mushroom bed extract.

2. 2. The antibacterial composition for cosmetics according to claim 1, wherein the target bacteria is at least one species selected from the group consisting of gram-negative bacteria, gram-positive bacteria and fungi.

3. A cosmetic comprising the antibacterial composition for cosmetics according to claim 1 or 2.

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