Combination of lingonberry, amla, and hibiscus

Abstract

To provide a combination of lingonberry, amla, and hibiscus.SOLUTION: The invention provides a combination of lingonberry, amla, and hibiscus.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a combination of cloudberry, amla and hibiscus.

Background Art

[0002] The skin is composed of the stratum corneum, epidermis, basement membrane and dermis from the outside. Among them, the dermis is the largest region, not as densely packed with cells as the epidermis. Rather, it has a lot of extracellular space and is filled with a network structure of macromolecules called the "extracellular matrix". This extracellular matrix is produced by fibroblasts in the dermis and is composed of polysaccharides called acidic mucopolysaccharides such as hyaluronic acid and dermatan sulfate, and fibrous proteins such as collagen and elastin. The extracellular matrix is directly involved in the elasticity, firmness, moisture, and metabolism of the skin. When the production of the extracellular matrix in fibroblasts and the like slows down, the elasticity and moisture of the skin are lost, and wrinkles, fine lines, and rough skin are likely to occur, contributing to skin aging.

[0003] The above fibrous protein mainly consists of collagen, and among them, type I collagen accounts for 80% of the whole. In addition to type I collagen, the presence of types III, V, XII, and XIV collagen is known. One of the causes of wrinkles and sagging seen in aging skin is the thinning of skin tissue with aging. In aging skin, the decrease in collagen fibers, which are the main matrix components of the dermis, is significant, and this is likely to be the main cause of the decrease in skin thickness. Therefore, it is considered effective in preventing and improving wrinkles and sagging to promote the production of collagen and maintain the amount of collagen. In addition to the above, promoting the production of collagen is also considered effective in improving wound healing of the skin and the like.

[0004] As components having such an action of promoting collagen production, various components have been conventionally known, such as N-benzoylglycylglycine (Patent Document 1), etc. On the other hand, there is still a demand for the development of components and products having a higher effect of promoting collagen production even now.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Summary of the Invention

[0006] The present inventors newly found a combination of lingonberry, amla and hibiscus. The present invention is based on this finding.

[0007] Therefore, the present invention provides a combination of lingonberry, amla and hibiscus.

[0008] According to the present invention, the following inventions are provided. (1) A composition comprising lingonberry, amla and hibiscus. (2) The composition according to (1) for promoting the production of collagen. (3) A composition for promoting the production of collagen, comprising lingonberry, which is used in combination with amla and hibiscus. (4) A composition for promoting the production of collagen, comprising amla, which is used in combination with lingonberry and hibiscus. (5) A composition for promoting the production of collagen, comprising hibiscus, which is used in combination with lingonberry and amla. A composition according to any one of (1) to (5) for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, softness of the skin, and gloss of the hair. A composition according to any one of (1) to (6) for use such that when the dry weight of Cornus officinalis Sieb. et Zucc. is CA and the dry weight of Phyllanthus emblica Linn. is CB, CA / CB is 0.2 to 2.5. A composition according to any one of (1) to (7) for use such that when the dry weight of Phyllanthus emblica Linn. is CB and the dry weight of Hibiscus sabdariffa Linn. is CD, CB / CD is 0.15 to 2.5. A composition according to any one of (1) to (8) for use such that when the dry weight of Cornus officinalis Sieb. et Zucc. is CA and the dry weight of Hibiscus sabdariffa Linn. is CD, CA / CD is 0.1 to 1. A composition according to any one of (1) to (9) which is an oral composition. Use of a combination of Cornus officinalis Sieb. et Zucc., Phyllanthus emblica Linn. and Hibiscus sabdariffa Linn. for the manufacture of a medicament for promoting the production of collagen. Use of a combination of Cornus officinalis Sieb. et Zucc., Phyllanthus emblica Linn. and Hibiscus sabdariffa Linn. for the manufacture of a medicament for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, softness of the skin, and gloss of the hair. Use according to (11) or (12) for use such that when the dry weight of Cornus officinalis Sieb. et Zucc. is CA and the dry weight of Phyllanthus emblica Linn. is CB, CA / CB is 0.2 to 2.5. Use according to any one of (11) to (13) for use such that when the dry weight of Phyllanthus emblica Linn. is CB and the dry weight of Hibiscus sabdariffa Linn. is CD, CB / CD is 0.15 to 2.5. Use according to any one of (11) to (14) for use such that when the dry weight of Cornus officinalis Sieb. et Zucc. is CA and the dry weight of Hibiscus sabdariffa Linn. is CD, CA / CD is 0.1 to 1. (16) The use according to any one of (11) to (15), wherein the cloudberry, amla or hibiscus is in the form of an oral composition. (17) Use of a combination of cloudberry, amla and hibiscus for promoting the production of collagen. (18) Use of a combination of cloudberry, amla and hibiscus for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of stretch marks, skin firmness or elasticity, skin softness, and hair gloss. (19) The use according to (17) or (18), wherein when the dry weight of cloudberry is CA and the dry weight of amla is CB, CA / CB is 0.2 to 2.5. (20) The use according to any one of (17) to (19), wherein when the dry weight of amla is CB and the dry weight of hibiscus is CD, CB / CD is 0.15 to 2.5. (21) The use according to any one of (17) to (20), wherein when the dry weight of cloudberry is CA and the dry weight of hibiscus is CD, CA / CD is 0.1 to 1. (22) The use according to any one of (17) to (21), wherein the cloudberry, amla or hibiscus is in the form of an oral composition. (23) A combination of cloudberry, amla and hibiscus for promoting the production of collagen. (24) A combination of cloudberry, amla and hibiscus for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of stretch marks, skin firmness or elasticity, skin softness, and hair gloss. (25) The combination according to (23) or (24), wherein when the dry weight of cloudberry is CA and the dry weight of amla is CB, CA / CB is 0.2 to 2.5. (26) When the dry weight of amla is CB and the dry weight of hibiscus is CD, the combination according to any one of (23) to (25) for use such that CB / CD is 0.15 to 2.5. (27) When the dry weight of cloudberry is CA and the dry weight of hibiscus is CD, the combination according to any one of (23) to (26) for use such that CA / CD is 0.1 to 1. (28) The combination according to any one of (23) to (27), wherein cloudberry, amla or hibiscus is in the form of an oral composition. (29) A method for promoting the production of collagen in a subject, the method comprising administering an effective amount of a combination of cloudberry, amla and hibiscus. (30) A method for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss in a subject, the method comprising administering an effective amount of a combination of cloudberry, amla and hibiscus. (31) The method according to (29) or (30) for administration such that when the dry weight of cloudberry is CA and the dry weight of amla is CB, CA / CB is 0.2 to 2.5. (32) The method according to any one of (29) to (31) for administration such that when the dry weight of amla is CB and the dry weight of hibiscus is CD, CB / CD is 0.15 to 2.5. (33) The method according to any one of (29) to (32) for administration such that when the dry weight of cloudberry is CA and the dry weight of hibiscus is CD, CA / CD is 0.1 to 1. (34) The method according to any one of (29) to (33), wherein cloudberry, amla or hibiscus is in the form of an oral composition.

[0009] According to the present invention, a combination of cloudberry, amla and hibiscus is provided. By using the combination of the present invention, it is also possible to effectively promote the production of collagen.

Brief Description of the Drawings

[0010]

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[0011] According to one aspect of the present invention, there is provided a composition comprising cloudberry, amla and hibiscus.

[0012] According to another aspect of the present invention, there is provided a collagen production promoter comprising cloudberry for use in combination with a composition comprising amla or amla and a composition comprising hibiscus or hibiscus. Such a collagen production promoter comprising cloudberry may be a composition comprising cloudberry for promoting the production of collagen.

[0013] According to another aspect of the present invention, there is provided a collagen production promoter comprising amla for use in combination with a composition comprising cloudberry or cloudberry and a composition comprising hibiscus or hibiscus. Such a collagen production promoter comprising amla may be a composition comprising amla for promoting the production of collagen.

[0014] According to another aspect of the present invention, there is provided a collagen production promoter comprising hibiscus for use in combination with a composition comprising cloudberry or cloudberry and a composition comprising amla or amla. Such a collagen production promoter comprising hibiscus may be a composition comprising hibiscus for promoting the production of collagen.

[0015] According to a preferred embodiment of the present invention, there is provided a collagen production promoter comprising cloudberry, amla and hibiscus. Such a collagen production promoter comprising cloudberry, amla and hibiscus may be a composition comprising cloudberry, amla and hibiscus for promoting the production of collagen.

[0016] The collagen in the present invention is not particularly limited, but is preferably type I collagen.

[0017] According to another aspect of the present invention, there is provided a composition comprising or including cloudberry, for use in combination with a composition comprising or including amla and a composition comprising or including hibiscus, for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss. According to a preferred embodiment of the present invention, there is provided a composition comprising or including cloudberry, for use in combination with a composition comprising or including amla and a composition comprising or including hibiscus, for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, and skin softness.

[0018] Such an improving agent comprising cloudberry may be a composition comprising cloudberry. Such improvement of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, or hair gloss may be achieved by promoting the production of collagen, or may be achieved without promoting the production of collagen, but is preferably achieved by promoting the production of collagen.

[0019] According to another aspect of the present invention, there is provided a composition comprising or including amla, for use in combination with a composition comprising or including cloudberry and a composition comprising or including hibiscus, for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss. According to a preferred embodiment of the present invention, there is provided a composition comprising or including amla, for use in combination with a composition comprising or including cloudberry and a composition comprising or including hibiscus, for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, and skin softness.

[0020] An improving agent comprising such amla may be a composition comprising amla. Such improvement of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, softness of the skin, or gloss of the hair may be achieved by promoting the production of collagen, or may be achieved without promoting the production of collagen, but is preferably achieved by promoting the production of collagen.

[0021] According to another aspect of the present invention, provided is an improving agent comprising hibiscus, for use in combination with a composition comprising lingonberry or a composition comprising amla, the improving agent being selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, softness of the skin, and gloss of the hair. According to a preferred embodiment of the present invention, provided is an improving agent comprising hibiscus, for use in combination with a composition comprising lingonberry or a composition comprising amla, the improving agent being selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, and softness of the skin.

[0022] An improving agent comprising such hibiscus may be a composition comprising hibiscus. Such improvement of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, firmness or elasticity of the skin, softness of the skin, or gloss of the hair may be achieved by promoting the production of collagen, or may be achieved without promoting the production of collagen, but is preferably achieved by promoting the production of collagen.

[0023] According to one preferred embodiment of the present invention, there is provided one or more improving agents selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss, comprising cloudberry, amla, and hibiscus. According to a more preferred embodiment of the present invention, there is provided one or more improving agents selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, and skin softness, comprising cloudberry, amla, and hibiscus.

[0024] Such an improving agent comprising cloudberry, amla, and hibiscus may be a composition comprising cloudberry, amla, and hibiscus. Such improvement of rough skin, skin moisture, crow's feet or fine wrinkles, prominent nasolabial folds, skin firmness or elasticity, skin softness, or hair gloss may be achieved by promoting the production of collagen, or may be achieved without promoting the production of collagen, but is preferably achieved by promoting the production of collagen.

[0025] The improvement in the present invention includes that the state of a subject that is not equivalent to that of a healthy person returns to or approaches the state equivalent to that of a healthy person, or that the state of a subject that is equivalent to that of a healthy person is maintained or becomes better than the state equivalent to that of a healthy person.

[0026] In the present invention, the subjects to which the combination of cloudberry, amla, and hibiscus is applied are not limited, and examples include mammals such as humans, primates including chimpanzees, pet animals such as dogs and cats, livestock animals such as cows, horses, sheep, and goats, and rodents such as mice and rats. The subject to which the combination of cloudberry, amla, and hibiscus in the present invention is applied is preferably a human.

[0027] The usage amounts of lingonberry and amla in the composition of the present invention can be appropriately determined according to the application. Preferably, when the dry weight of lingonberry is CA and the dry weight of amla is CB, CA / CB is 0.2 to 2.5, and more preferably CA / CB is 0.5 to 1.5.

[0028] The usage amounts of amla and hibiscus in the composition of the present invention can be appropriately determined according to the application. Preferably, when the dry weight of amla is CB and the dry weight of hibiscus is CD, CB / CD is 0.15 to 2.5, and more preferably CB / CD is 0.25 to 0.8.

[0029] The usage amounts of lingonberry and hibiscus in the composition of the present invention can be appropriately determined according to the application. Preferably, when the dry weight of lingonberry is CA and the dry weight of hibiscus is CD, CA / CD is 0.1 to 1, and more preferably CA / CD is 0.2 to 0.6.

[0030] The usage amounts of lingonberry, amla and hibiscus in the composition of the present invention can be appropriately determined according to the application, and the usage amounts of lingonberry and amla, amla and hibiscus, and lingonberry and hibiscus defined above may be appropriately combined.

[0031] "Lingonberry" in the present invention means Vaccinium vitis-idaea (Chinese name: bilberry), a plant of the genus Vaccinium in the family Ericaceae, and is also called cowberry. Generally, lingonberry grows abundantly in the Arctic regions of northern Europe and Canada and is known to be rich in vitamin C and citric acid. The fruits are eaten raw and are commonly used in processed foods such as juice and jam. In the present invention, common lingonberry can be used.

[0032] In the present invention, as the part of lingonberry, for example, fruits (immature fruits, ripe fruits, dried fruits), leaves, tubers, flowers, seeds, etc. can be used, and preferably fruits are used.

[0033] In the present invention, the sea buckthorn can be used either fresh or dried. According to one aspect of the present invention, "sea buckthorn" is a sea buckthorn extract produced using the whole or a part of the sea buckthorn.

[0034] "Amla" in the present invention refers to Phyllanthus emblica or Emblica officinale (Chinese name: Amla), which is a plant of the genus Emblica in the Euphorbiaceae family, and is also called Indian gooseberry, oil plum, amalaki, Malacca tree, etc. In the present invention, common amla can be used.

[0035] In the present invention, as parts of amla, for example, fruits (immature fruits, ripe fruits, dried fruits), leaves, tubers, flowers, seeds, etc. can be used, and preferably fruits are used. Also, in the present invention, amla itself (for example, fresh fruits) can be used.

[0036] In the present invention, amla can be used either fresh or dried. According to one aspect of the present invention, "amla" is an amla extract produced using the whole or a part of amla.

[0037] "Hibiscus" in the present invention refers to Hibiscus sabdariffa or Hibiscus rosa-sinensis (Chinese name: Luo Shen Hua), which is a plant of the genus Hibiscus in the Malvaceae family, and is also called roselle and shoe flower, respectively. Generally, hibiscus is a plant native to Africa, and because of its refreshing sour taste, it is also used as a sauce in French cuisine and Italian cuisine, and is also used as a herb. In the present invention, common hibiscus can be used, but preferably roselle is used.

[0038] In the present invention, as the parts of hibiscus, for example, flowers, sepals, buds, fruits, pericarp, seeds, seed coats, stems, leaves, branches, branch leaves, trunks, barks, roots, rhizomes, root barks, etc. can be used, and preferably sepals and / or flowers are used.

[0039] In the present invention, hibiscus can be used in its raw state, dried state, or extracted with a solvent. According to one aspect of the present invention, "hibiscus" is a hibiscus extract produced using the whole or a part of hibiscus.

[0040] The method for producing the cloudberry extract, amla extract or hibiscus extract in the present invention is not particularly limited, and can be produced according to the methods commonly used in the art. For example, it may be carried out by extracting the raw material cloudberry, amla or hibiscus. Examples of the extraction method include, but are not limited to, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, solvent extraction method, etc. These can be carried out alone or in combination of two or more methods. Further, the extract may be further extracted one or more times by the same method in order to obtain a high-purity extract.

[0041] The type of solvent used for the production of the cloudberry extract, amla extract or hibiscus extract in the present invention is not particularly limited, and any solvent known in the art may be used as long as an extract having the object and effect of the present invention can be obtained. Examples of such solvents include, but are not limited to, water, hexane, ether, ethyl acetate, butanol, acetone, propanol, ethanol, methanol, propylene glycol, 1,3-butylene glycol, etc., and these may be used in combination of two or more.

[0042] If cloudberries (including dried ones, extracts, etc.), amlas (including dried ones, extracts, etc.) or hibiscuses (including dried ones, extracts, etc.) are commercially available, they may be used as long as they have the objective effects of the present invention.

[0043] The composition of the present invention is not limited, but may be any of a pharmaceutical composition, a cosmetic composition, and a food composition. Preferably, the composition in the present invention is a food composition.

[0044] The composition of the present invention may be either an oral composition or a topical composition. Cloudberries, amlas, and hibiscuses in the present invention are preferably absorbed from the digestive tract by oral ingestion, and their active ingredients act in the body. Therefore, according to one preferred embodiment of the present invention, the composition of the present invention is an oral composition.

[0045] When the composition of the present invention is an oral composition, known ingredients (additives) formulated in the oral composition can be further formulated. Such ingredients are not particularly limited, but examples include emulsifiers, hydrating agents, solvents, emollients, stabilizers, thickeners, preservatives, lubricants, chelating agents, fillers, excipients, powders, fragrances, flavorings, absorbents, dyes, opacifiers, antioxidants, antiseptics, vitamins, amino acids, nutrients, minerals (electrolytes), flavorings such as synthetic flavorings and natural flavorings, pectic acid and its salts, alginic acid and its salts, organic acids, pH adjusters, glycerin, alcohols, carbonating agents used in carbonated beverages, and the like.

[0046] The oral composition in the present invention may be in any form of liquid, semi-solid, or solid.

[0047] When the composition of the present invention is used as a topical composition, known components (additives) formulated in the topical composition can be further formulated. Such components include, but are not limited to, for example, emulsifiers, humectants, solvents, emollients, stabilizers, thickeners, preservatives, lubricants, chelating agents, fillers, excipients, powders, fragrances, flavorings, absorbents, dyes, opacifiers, antioxidants, vitamins, amino acids, and the like.

[0048] The topical composition in the present invention may be in any form of liquid, semi-solid, or solid.

Examples

[0049] The present invention will be specifically described based on the following examples, but the present invention is not limited to these examples. Unless otherwise specified, the content is expressed in mass%.

[0050] Cell culture Commercially available human neonatal dermal fibroblasts (Cryo NHDF-Neo, Sanko Junyaku) were used for the cells. Human neonatal dermal fibroblasts were seeded in a commercially available 24-well plate at a density of 2×10 5 cells per well, and cultured at 37°C, 5% CO 2 and saturated water vapor atmosphere until confluent using a medium prepared by adding 10% fetal bovine serum to a commercially available cell culture medium (D-MEM (1 g / L glucose), Wako Pure Chemical Industries). After culturing until confluence, the medium in each well was replaced with a drug-added medium prepared such that the dry weight of the hibiscus extract was 0.3 μg / mL, or the dry weight of the lingonberry extract was 0.17 μg / mL and the dry weight of the amla extract was 0.6 μg / mL, or the dry weight of the lingonberry extract was 0.08 μg / mL, the dry weight of the amla extract was 0.3 μg / mL, and the dry weight of the hibiscus extract was 0.15 μg / mL in the same medium, and incubated again for 72 hours. In the control group, the same operation was performed after replacing with a drug-free cell culture medium (D-MEM (1 g / L glucose), Wako Pure Chemical Industries).

[0051] Drugs applied to cells As the agent, those described in Table 1 below were used.

Table 1

[0052] After culturing for 72 hours, the supernatant of the culture solution in each well was collected, and the concentration of the C-terminal peptide of type I procollagen (hereinafter referred to as "PIP") produced by human neonatal dermal fibroblasts in the collected supernatant was measured by ELISA using a Procollagen type I C-peptide EIA kit (manufactured by Takara Bio Inc.).

[0053] Measurement of PIP concentration by ELISA method The measurement by ELISA was performed according to the following procedure in accordance with the kit manual. After adding 100 μL of the labeled antibody solution to each well of the plate, 20 μL of the PIP standard solution and the measurement sample (supernatant) at each prepared concentration were added to each well with a micropipette, and reacted at 37°C for 3 hours. At this time, the measurement sample was added within 5 minutes. After discarding the reaction solution and washing 4 times with PBS, 100 μL of Substrate Solution (TMBZ) was added to each well and reacted at room temperature for 15 minutes. 100 μL of Stop Solution was added to each well in the order in which Substrate Solution (TMBZ) was added to stop the reaction, and then thoroughly mixed. Zero adjustment was made using distilled water as a control, and the absorbance was measured at a wavelength of 450 nm. The color development was stable until 1 hour after the reaction was stopped. A standard curve was created by plotting the concentration of each PIP standard solution on the horizontal axis and the corresponding absorbance on the vertical axis, and the value of the PIP concentration corresponding to the absorbance of the measurement sample was taken as the measurement result. The measurement result was corrected by the number of cells in each well obtained by alamar blue measurement to obtain the value of the collagen production amount.

[0054] Statistical analysis was performed using the Tukey-Kramer method as a multiple comparison test to determine whether there was a significant improvement in the groups being compared. A p-value < 0.05 was considered significant (*).

[0055] The evaluation results of the collagen production amount in human neonatal dermal fibroblasts are shown in Figure 1. It was shown that all of the groups to which hibiscus was applied alone, the group to which raspberry and amla were applied in combination, and the group to which hibiscus, raspberry, and amla were applied in combination significantly promoted collagen production compared to the control group. Also, the group to which hibiscus, raspberry, and amla were applied in combination significantly promoted collagen production compared to both the control group and the group to which hibiscus was applied alone, and it was shown that there was a tendency to more significantly promote collagen production compared to the group to which raspberry and amla were applied in combination.

[0056] Evaluation of the effects of lingonberry, amla and hibiscus on the condition of human skin, etc. A combined use test was conducted by panelists, and the effects of the present invention were confirmed by the following tests. For the combined use test, four types of test samples (tablets) were prepared and implemented in groups A to D. The number of panelists was 15 in group A (placebo group), 15 in group B (72 mg (dry weight) of hibiscus extract per day), 15 in group C (25 mg (dry weight) of raspberry extract + 30 mg (dry weight) of amla extract per day), and 15 in group D (12.5 mg (dry weight) of raspberry extract + 15 mg (dry weight) of amla extract + 36 mg (dry weight) of hibiscus extract per day). The panelists were healthy Japanese women aged 35 to 50 who excluded those who met one or more of the conditions described in Table 2 below.

Table 2

[0057] Each panelist took the test sample (tablet) orally once a day, every day for 28 days, before going to bed with water or warm water. For the tablet, the cloudberry extract listed in Table 2 was used as the cloudberry, the amla extract listed in Table 2 was used as the amla, and the hibiscus extract listed in Table 2 was used as the hibiscus.

[0058] Questionnaire evaluation (Test method) Before the start of the combination test and 1 day, 3 days, 7 days, 14 days, 21 days, and 28 days after the start of the combination test, a questionnaire survey was conducted on items related to the condition of human skin and the like.

[0059] The questionnaire was evaluated using the VAS (abbreviation for Visual Analogue Scale), which is widely used as an evaluation scale. That is, on each survey day, a piece of paper with a 10-cm horizontal straight line drawn on it was distributed to the subjects. The left end of the straight line was marked as "the worst condition" and the right end as "the best condition", and the subjects were asked to mark the degree of actual feeling as a position on the straight line (the subject makes a mark at one point on the line segment). The distance moved to the right (or left) from the starting position of the test was used as the scale value for evaluation. The results of "rough skin" are shown in Figure 2, the results of "skin moisture" are shown in Figure 3, the results of "crow's feet or fine wrinkles" are shown in Figure 4, the results of "prominent stretch marks" are shown in Figure 5, the results of "skin firmness or elasticity" are shown in Figure 6, the results of "skin softness" are shown in Figure 7, and the results of "hair gloss" are shown in Figure 8. In Figures 2 to 8, P represents the evaluation result of Group A (placebo), H represents the evaluation result of Group B (hibiscus), L+A represents the evaluation result of Group C (cloudberry + amla), and H+L+A represents the evaluation result of Group D (cloudberry + amla + hibiscus).

[0060] From the results of FIGS. 2 to 8, Group D (goji berry + amla + hibiscus) showed an improvement effect on rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss compared to any of Group A (placebo), Group B (hibiscus), and Group C (goji berry + amla). Also, such an effect of Group D (goji berry + amla + hibiscus) was higher than the simple sum of the effects of Group B (hibiscus) and Group C (goji berry + amla), indicating that the combination of goji berry, amla, and hibiscus has a synergistic effect on the improvement of rough skin, skin moisture, crow's feet or fine wrinkles, prominence of nasolabial folds, skin firmness or elasticity, skin softness, and hair gloss.

Claims

1. A composition comprising cloudberry, amla and hibiscus.

2. The composition according to claim 1 for promoting the production of collagen.

3. A composition for promoting the production of collagen, comprising cloudberry, for use in combination with amla and hibiscus.

4. A composition for promoting the production of collagen, comprising amla, for use in combination with cloudberry and hibiscus.

5. A composition for promoting the production of collagen, comprising hibiscus, for use in combination with cloudberry and amla.

6. The composition according to any one of claims 1 to 5 for improving one or more selected from the group consisting of rough skin, skin moisture, crow's feet or fine wrinkles, prominent stretch marks, skin firmness or elasticity, skin softness, and hair gloss.

7. The composition according to any one of claims 1 to 5, for use such that when the dry weight of cloudberry is CA and the dry weight of amla is CB, CA / CB is 0.2 to 2.

5.

8. The composition according to any one of claims 1 to 5, for use such that when the dry weight of amla is CB and the dry weight of hibiscus is CD, CB / CD is 0.15 to 2.

5.

9. The composition according to any one of claims 1 to 5, for use such that when the dry weight of cloudberry is CA and the dry weight of hibiscus is CD, CA / CD is 0.1 to 1.

10. The composition according to any one of claims 1 to 5, for use such that when the dry weight of cloudberry is CA, the dry weight of amla is CB, and the dry weight of hibiscus is CD, CA / CB is 0.2 to 2.5 and CB / CD is 0.15 to 2.

5.

11. The composition according to any one of claims 1 to 5, which is an oral composition.

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