Composition for biofilm degradation

Abstract

To provide a composition for biofilm degradation with enhanced ability to degrade biofilms, compared to traditional technologies.SOLUTION: A composition for biofilm degradation comprises (A) sucrose fatty acid ester.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a composition for decomposing biofilms. [Background technology]

[0002] A biofilm, also called a bacterial membrane, is a structure formed by microorganisms such as bacteria. A biofilm is generally a film-like structure formed by bacterial aggregates covered with glycocalyx, which is made of exopolysaccharides, attached to the surface of a substance. Biofilms can be formed anywhere where microorganisms such as bacteria are present, but they are particularly often formed in the oral cavity of humans and animals, and are one of the causes of oral diseases such as dental caries and periodontal disease.

[0003] Conventionally, in order to prevent oral diseases such as dental caries and periodontal disease, methods have been proposed for killing bacteria that form biofilms using bactericides, antibacterial agents, antibiotics, etc., as well as methods for inhibiting bacterial adhesion and the formation of bacterial aggregates during the biofilm formation process (e.g., Patent Document 1).

[0004] However, once a biofilm is formed, its surface has a barrier function, so that it is generally difficult to kill bacteria inside the biofilm and to remove the biofilm itself using only conventional antibacterial agents. In addition, attempts have been made to remove biofilms using physical actions such as brushing with a toothbrush or using ultrasound, but it has been difficult to sufficiently remove biofilms even with physical actions. Therefore, it remains a continuing technical challenge to provide a method for removing formed biofilms.

[0005] As a method for removing the formed biofilm, a method has been proposed in which an oral composition containing a sugar alcohol or an amino acid is applied to the formed biofilm (for example, Patent Document 2).

[0006] However, the reality is that conventional techniques are not sufficiently effective in removing biofilms. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] Japanese Patent Application Publication No. 6-24948 [Patent Document 2] JP 2005-29484 A Summary of the Invention [Problem to be solved by the invention]

[0008] Under these circumstances, there remains a continuing technical challenge to provide a composition for decomposing biofilms that has improved biofilm removal ability compared to conventional techniques.

[0009] Therefore, an object of the present invention is to provide a composition for biofilm decomposition having improved biofilm decomposition ability as compared to the conventional techniques. Another object of the present invention is to provide a method for decomposing biofilms more efficiently as compared to the conventional techniques. [Means for solving the problem]

[0010] As a result of intensive research, the present inventors have found that the above-mentioned problems can be solved by adding (A) a sucrose fatty acid ester to a composition for decomposing biofilms. The present invention is based on this finding.

[0011] That is, according to the present invention, the following inventions are provided. [1] Component (A) A composition for decomposing biofilms, comprising a sucrose fatty acid ester. [2] The composition for decomposing biofilms described in [1], further comprising one or more components selected from the group consisting of silicon compounds, phosphate compounds, hydroxy acid compounds, amino acids, carbonate compounds and salts thereof as component (B). [3] The composition for decomposing biofilms described in [2], wherein the silicon compound contains one or more types selected from the group consisting of zeolite and silica. [4] The composition for decomposing biofilms described in [2], wherein the phosphate compound contains one or more compounds selected from the group consisting of polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid, phosphoric acid, and salts thereof. [5] The composition for decomposing biofilms described in [2], wherein the hydroxy acid compound contains one or more selected from the group consisting of citric acid, gluconic acid and salts thereof. [6] The composition for decomposing a biofilm according to [2], wherein the amino acid contains one or more selected from the group consisting of glutamic acid, aspartic acid and salts thereof. [7] The composition for decomposing a biofilm according to [2], wherein the carbonate compound contains calcium carbonate. [8] The composition for decomposing a biofilm according to any one of [1] to [7], wherein the fatty acid group constituting the component (A) has 14 to 18 carbon atoms. [9] The composition for decomposing biofilms described in any of [1] to [8], wherein the component (A) contains either one or both of sucrose stearate and sucrose palmitate.

[10] The composition for decomposing a biofilm according to any one of [1] to [9], wherein the HLB value of the component (A) is 10 or more.

[11] The composition for decomposing biofilms according to any of [1] to

[10] , wherein the content of component (A) is 0.01 to 15 mass% relative to the total mass of the composition for decomposing biofilms.

[12] The composition for decomposing biofilms according to any one of [2] to

[11] , wherein the content of component (B) is 0.01 to 15 mass% relative to the total mass of the composition for decomposing biofilms.

[13] The composition for decomposing biofilms according to any one of [1] to

[12] , which is for use in the oral cavity.

[14] The composition for decomposing biofilms described in any of [1] to

[12] , which is a dentifrice, a mouthwash or an oral moisturizer. Effect of the Invention

[0012] According to the present invention, it is possible to improve the biofilm decomposition ability of a composition for biofilm decomposition compared to the conventional technology. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] [Biofilm decomposition composition] According to one aspect of the present invention, there is provided a composition for biofilm decomposition having improved biofilm decomposition ability compared to the conventional techniques (hereinafter also referred to as "the composition for biofilm decomposition of the present invention"). The composition for biofilm decomposition of the present invention contains component (A) a sucrose fatty acid ester, and optionally contains component (B) one or more selected from the group consisting of silicon compounds, phosphate compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof. Each component will be described in detail below.

[0014] Component (A) The composition for decomposing a biofilm of the present invention contains, as an essential component, component (A) a sucrose fatty acid ester. The composition for decomposing a biofilm contains a sucrose fatty acid ester, thereby exerting the ability to decompose a biofilm. The sucrose fatty acid ester may be used alone or in combination of two or more kinds.

[0015] Sucrose fatty acid esters are nonionic surfactants that have sucrose as the hydrophilic group and fatty acid as the lipophilic group. Sucrose has eight hydroxyl groups in its molecule, and the sucrose fatty acid esters in the present invention may be any of monoesters to octaesters, or mixtures thereof.

[0016] The fatty acid constituting the sucrose fatty acid ester may be any of a short-chain fatty acid, a medium-chain fatty acid, a long-chain fatty acid and a very long-chain fatty acid, and the specific number of carbon atoms is not particularly limited as long as the effects of the present invention are achieved, and is preferably 14 to 18, and more preferably 16 to 18. By making the number of carbon atoms of the fatty acid constituting the sucrose fatty acid ester 14 to 18, it is possible to impart a more effective biofilm decomposition ability to the composition for biofilm decomposition.

[0017] The degree of unsaturation of the fatty acid constituting the sucrose fatty acid ester is not particularly limited as long as the effects of the present invention are achieved, and may be either a saturated fatty acid or an unsaturated fatty acid. In addition, the structure of the fatty acid constituting the sucrose fatty acid ester may be any of a linear structure, a branched structure, and a cyclic structure.

[0018] The HLB (Hydrophilic-Lipophilic Balance) value of the sucrose fatty acid ester may be any value from 0 to 20 as long as the effects of the present invention are achieved, and is preferably 10 or more, more preferably 10 to 18, and even more preferably 11 to 16. When the sucrose fatty acid ester has an HLB value of 10 to 18, it is possible to impart a more effective biofilm decomposition ability to the composition for biofilm decomposition.

[0019] The sucrose fatty acid ester preferably contains either or both of sucrose stearate and sucrose palmitate.

[0020] The content of component (A) in the composition for biofilm decomposition of the present invention is not particularly limited as long as the effects of the present invention are achieved, but is preferably 0.01 to 15 mass%, more preferably 0.1 to 10 mass%, and even more preferably 1 to 5 mass%, based on the total mass of the composition for biofilm decomposition. When the content of component (A) in the composition for biofilm decomposition is 0.01 mass% or more, the composition for biofilm decomposition can be imparted with a more effective biofilm decomposition ability. On the other hand, when the content of component (A) in the composition for biofilm decomposition is 15 mass% or less, the feel of the composition for biofilm decomposition in use can be improved.

[0021] Ingredient (B) The composition for biofilm decomposition of the present invention may, if necessary, further contain component (B) one or more selected from the group consisting of silicon compounds, phosphate compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof.

[0022] Examples of silicon compounds and their salts include sodium aluminosilicate, potassium aluminosilicate, calcium aluminosilicate, silica, anhydrous silicic acid, and hydrous silicic acid. In addition, minerals such as zeolite, mullite, kaolinite, illite, and feldspar can also be used as silicon compounds and their salts. Silicon compounds and their salts may be used alone or in combination of two or more.

[0023] Examples of the phosphoric acid compound and its salt include polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid, phosphoric acid, and their salts, etc. The phosphoric acid compound and its salt may be used alone or in combination of two or more.

[0024] Examples of hydroxy acid compounds and salts thereof include citric acid, gluconic acid, and salts thereof. The hydroxy acid compounds and salts thereof may be used alone or in combination of two or more.

[0025] Examples of the amino acid and its salt include glutamic acid, aspartic acid, and their salts. The amino acid and its salt may be used alone or in combination of two or more.

[0026] Examples of the carbonate compound and its salt include carbonic acid and its salt, and examples of the carbonate include calcium carbonate, sodium carbonate, sodium hydrogen carbonate, etc. The carbonate compound and its salt may be used alone or in combination of two or more.

[0027] The content of component (B) in the composition for biofilm decomposition of the present invention is not particularly limited as long as the effect of the present invention is exhibited, and can be appropriately set. When the component (B) contains a silicon compound and / or a salt thereof, the content is preferably 0.01 to 15 mass%, more preferably 0.1 to 10 mass%, and even more preferably 1 to 5 mass% based on the total mass of the composition for biofilm decomposition. When the composition for biofilm decomposition of the present invention contains two or more kinds of silicon compounds and their salts, the above-mentioned content is the total amount of the two or more kinds of silicon compounds and their salts. When the content of the silicon compound and its salt in the composition for biofilm decomposition is 0.01 mass% or more, the biofilm decomposition ability of the composition for biofilm decomposition can be more effectively enhanced. On the other hand, when the content of the silicon compound in the composition for biofilm decomposition is 15 mass% or less, the feeling of use of the composition for biofilm decomposition can be improved.

[0028] When the component (B) contains a phosphoric acid compound, a hydroxy acid compound, an amino acid, a carbonate compound, and / or a salt thereof, the content is preferably 0.01 to 15 mass%, more preferably 0.1 to 10 mass%, and even more preferably 0.5 to 5 mass%, based on the total mass of the composition for biofilm decomposition. When the composition for biofilm decomposition of the present invention contains two or more phosphoric acid compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof, the above-mentioned content is the total amount of the two or more phosphoric acid compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof. When the content of the phosphoric acid compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof in the composition for biofilm decomposition is 0.01 mass% or more, the biofilm decomposition ability of the composition for biofilm decomposition can be more effectively enhanced. On the other hand, when the content of the phosphoric acid compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof in the composition for biofilm decomposition is 15 mass% or less, the feeling of use of the composition for biofilm decomposition can be improved.

[0029] The ratio of the mass of component (B) to the mass of component (A) in the composition for biofilm decomposition of the present invention (mass of component (B) / mass of component (A)) is not particularly limited as long as the effects of the present invention are achieved, but is preferably 0.00067 to 1500, more preferably 0.01 to 100, and even more preferably 0.1 to 5. When the ratio of the mass of component (B) to the mass of component (A) in the composition for biofilm decomposition is 0.00067 to 1500, the composition for biofilm decomposition can be imparted with a more effective biofilm decomposition ability.

[0030] (Other Ingredients) In addition to the above-mentioned components (A) and (B), the composition for biofilm decomposition of the present invention may contain other components as necessary, such as abrasives, wetting agents, solvents, binders, flavors, excipients, sweeteners, pH adjusters, preservatives, emulsifiers, solubilizers, foaming agents, lubricants, oils, surfactants, chelating agents, dyes, pigments, antioxidants, flavoring agents, and other drugs. These other components are commonly used in pharmaceutical compositions, oral compositions, or food formulation designs, and can be appropriately selected within a range that does not impair the effects of the present invention, and can be blended in an appropriate amount in the composition for biofilm decomposition. These other components may be used alone or in combination of two or more.

[0031] Examples of the abrasive include calcium carbonate, calcium phosphate, dibasic calcium phosphate, calcium pyrophosphate, insoluble calcium metaphosphate, hydrous silicic acid, anhydrous silicic acid, titanium dioxide, amorphous silica, crystalline silica, aluminosilicate, aluminum oxide, aluminum hydroxide, and resin. One type of abrasive may be used alone, or two or more types may be used in combination. The content of the abrasive is, for example, 3 to 60% by mass based on the total mass of the composition for decomposing biofilm of the present invention.

[0032] Examples of the humectant include polyhydric alcohols such as glycerin, sorbitol, polyethylene glycol, propylene glycol, ethylene glycol, 1,3-butylene glycol, polypropylene glycol, xylitol, maltitol, and lactitol. One type of humectant may be used alone, or two or more types may be used in combination. The content of the humectant is, for example, 0.1 to 50% by mass relative to the total mass of the composition for decomposing biofilm of the present invention.

[0033] Examples of the solvent include water and organic solvents (e.g., alcohols such as ethanol, propyl alcohol, and isopropyl alcohol), with water being particularly preferred. The solvent may be used alone or in combination of two or more. These alcohols may be blended alone or in combination of two or more. The content of the solvent is, for example, 99% by mass or less based on the total mass of the composition for decomposing biofilm of the present invention.

[0034] Examples of binders include cellulose derivatives such as carrageenan, carboxymethylcellulose, sodium carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, and hydroxypropylmethylcellulose, alkali metal alginates such as sodium alginate, gums such as xanthan gum, tragacanth gum, and gum arabic, synthetic binders such as polyvinyl alcohol and sodium polyacrylate, and inorganic binders such as silica gel, aluminum silica gel, and Veegum. One type of binder may be used alone, or two or more types may be used in combination. The content of the binder is, for example, 0.5 to 10% by mass with respect to the total mass of the composition for decomposing biofilm of the present invention.

[0035] The flavoring may be a known flavoring material, for example, a blended flavoring such as strawberry flavor, apple flavor, banana flavor, pineapple flavor, grape flavor, mango flavor, butter flavor, milk flavor, fruit mix flavor, or tropical fruit flavor. The flavoring may be used alone or in combination of two or more. The content of the flavoring is, for example, 0.0001 to 1.0% by mass based on the total mass of the composition for decomposing biofilm of the present invention.

[0036] Examples of excipients include sucrose, lactose, starch, glucose, crystalline cellulose, mannitol, sorbitol, xylitol, erythritol, palatinit, palatinose, maltitol, trehalose, lactitol, lactulose, reduced starch sugar, reduced isomaltooligosaccharide, coupling sugar, gum base, gum arabic, gelatin, cetyl methylcellulose, light anhydrous silicic acid, magnesium aluminate, calcium aluminometasilicate, sodium bicarbonate, calcium phosphate, etc. One type of excipient may be used alone, or two or more types may be used in combination. The content of the excipient is, for example, 0.01 to 30% by mass with respect to the total mass of the composition for decomposing biofilm of the present invention.

[0037] Examples of sweeteners include palatinit, aspartame, saccharin sodium, acesulfame potassium, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perillarmine, thaumatin, aspartyl phenylalanyl methyl ester, and p-methoxycinnamic aldehyde. One type of sweetener may be used alone, or two or more types may be used in combination. The content of the sweetener is, for example, 0.01 to 1% by mass relative to the total mass of the composition for decomposing biofilm of the present invention.

[0038] Examples of pH adjusters include citric acid, phosphoric acid, pantothenic acid, malic acid, pyrophosphoric acid, lactic acid, tartaric acid, glycerophosphoric acid, acetic acid, nitric acid, and chemically possible salts thereof such as disodium hydrogen phosphate, and sodium hydroxide. One type of pH adjuster may be used alone, or two or more types may be used in combination. The content of the pH adjuster is, for example, 0.01 to 2% by mass relative to the total mass of the composition for decomposing biofilm of the present invention.

[0039] Examples of preservatives include paraoxybenzoic acid esters, benzoic acid and its salts, salicylic acid and its salts, sorbic acid and its salts, phenoxyethanol, alkyldiaminoethylglycine hydrochloride, etc. One preservative may be used alone, or two or more preservatives may be used in combination. The content of the preservative is, for example, 0.005 to 5% by mass based on the total mass of the composition for decomposing biofilm of the present invention.

[0040] Examples of emulsifiers include polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, sodium stearoyl lactylate, soybean phospholipids, and alkyl trimethyl ammonium chloride. One type of emulsifier may be used alone, or two or more types may be used in combination. The content of the emulsifier is, for example, 0.05 to 30% by mass relative to the total mass of the composition for decomposing biofilm of the present invention.

[0041] Examples of solubilizing agents include esters, polyethylene glycol derivatives, polyoxyethylene hydrogenated castor oil, fatty acid esters of sorbitan, and sulfated fatty alcohols. One solubilizing agent may be used alone, or two or more solubilizing agents may be used in combination. The content of the solubilizing agent is, for example, 0.05 to 30% by mass relative to the total mass of the composition for decomposing biofilm of the present invention.

[0042] Examples of foaming agents include sodium lauryl sulfate, sodium lauroyl sarcosine, sodium alkyl sulfosuccinate, sodium coconut oil fatty acid monoglycerin sulfonate, sodium α-olefin sulfonate, N-acylamino acid salts such as N-acyl glutamate, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, maltitol fatty acid ester, sucrose fatty acid ester, polyglycerin fatty acid ester, fatty acid diethanolamide, polyoxyethylene sorbitan monostearate, polyoxyethylene hydrogenated castor oil, polyoxyethylene fatty acid ester, etc. The foaming agents may be used alone or in combination of two or more.

[0043] Examples of lubricants include magnesium stearate, sucrose fatty acid esters, talc, and hardened oils.

[0044] Examples of the oil include terpenoid essential oils used as antibacterial components (e.g., peppermint oil, eucalyptus oil, orange oil, turpentine oil, peppermint oil, spearmint oil, camphor oil, thyme oil, perilla oil, tea tree oil, lemon oil, sage oil, rosemary oil, 1-limonene, 1-menthol, menthone, α-pinene, citral, cineole, camphor, etc.) and phenylpropanoid essential oils (e.g., clove oil, fennel oil, cinnamon oil, eugenol, anethole, cinnamaldehyde, cinnamaldehyde, etc.), and oils other than the above-mentioned oils used as flavorings, such as coconut oil, olive oil, sesame oil, peanut oil, parsley oil, parsley seed oil, safflower oil, etc. One type of oil may be used alone, or two or more types may be used in combination.

[0045] Examples of surfactants include sodium lauryl sulfate, sodium α-olefin sulfonate, sodium dodecylbenzenesulfonate, sodium lauryl sulfoacetate, sodium N-lauroyl sarcosinate, N-acyl glutamate, polyoxyethylene hydrogenated castor oil, polyoxyethylene polyoxypropylene block copolymer, alkyl glycosides, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, alkyl dimethylamine oxide, lauryl ethanolamide, sodium cocoyl sarcosinate, and sodium N-lauroyl methyl taurate solution. One type of surfactant may be used alone, or two or more types may be used in combination. The content of the surfactant is, for example, 0 to 30% by mass with respect to the total mass of the biofilm decomposition composition of the present invention.

[0046] Examples of the chelating agent include pyrophosphoric acid or a salt thereof, polyphosphoric acid or a salt thereof, metaphosphoric acid or a salt thereof, phytic acid or a salt thereof, hydroxyethanediphosphonic acid or a salt thereof, aminotrimethylenephosphonic acid or a salt thereof, ethylenediaminetetramethylenephosphonic acid or a salt thereof, phosphonobutanetricarboxylic acid or a salt thereof, edetic acid (EDTA) or a salt thereof, nitrilotriacetic acid (NTA) or a salt thereof, hydroxyethyliminodiacetic acid (HIDA) or a salt thereof, tetrasodium 3-hydroxy-2,2'-iminodisuccinate (HIDS) or a salt thereof, diethylenetriaminepentaacetic acid (DPTA) or a salt thereof, hydroxyethyl Examples of the chelating agent include ethylenediaminetriacetic acid (HEDTA) or a salt thereof, dihydroxyethylglycine (DHEG) or a salt thereof, L-glutamic acid diacetate (GLDA) or a salt thereof, methylglycine diacetate (MGDA), aspartic acid or a salt thereof, glutamic acid or a salt thereof, citric acid or a salt thereof, malic acid or a salt thereof, tartaric acid or a salt thereof, gluconic acid or a salt thereof, lactic acid or a salt thereof, mandelic acid or a salt thereof, glycolic acid or a salt thereof or a salt thereof, oxalic acid or a salt thereof, maleic acid or a salt thereof, malonic acid or a salt thereof, succinic acid or a salt thereof, pantothenic acid or a salt thereof, phthalic acid or a salt thereof, and fumaric acid or a salt thereof. One type of chelating agent may be used alone, or two or more types may be used in combination. The content of the chelating agent is, for example, 0 to 30% by mass with respect to the total mass of the composition for decomposing biofilm of the present invention.

[0047] In addition to the above-mentioned ingredients, the composition may contain, for example, colorants such as Blue No. 1, pigments such as titanium dioxide, antioxidants such as dibutylhydroxytoluene, tea extract, tea distillate, flavoring agents such as monosodium glutamate, etc.

[0048] The biofilm decomposition composition of the present invention can further contain medicinal ingredients such as moisturizers, antibacterial agents, anti-inflammatory agents, fluorides, vitamins, herbal extracts, etc. These medicinal ingredients can be appropriately selected from those that can be used in pharmaceuticals, foods, cosmetics, and oral compositions.

[0049] Examples of moisturizing agents include amino acids and their salts, pyrrolidone carboxylic acid, mucin, hyaluronic acid and its salts, mucopolysaccharides such as chondroitin sulfate, sodium lactate, urea, panthenol, natural extract components such as aloe extract, rosemary extract, thyme extract, tea extract (tea distillation extract), and so-called extracellular matrices such as collagen and elastin. One type of moisturizing agent may be used alone, or two or more types may be used in combination.

[0050] Examples of antibacterial agents include cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, dequalinium chloride, chlorhexidine hydrochloride, chlorhexidine sodium gluconate, bisabolol chlorhexidine, lactoferrin, paraben, butylparaben, etc. Antibacterial agents may be used alone or in combination of two or more.

[0051] Examples of anti-inflammatory agents include lysozyme chloride, ε-aminocaproic acid, aluminum hydroxyl allantoin, glycyrrhetinic acid, glycyrrhizinate salts, guaiazulene sulfonic acid, dl-α-tocopherol acetate, etc. Anti-inflammatory agents may be used alone or in combination of two or more.

[0052] Examples of the fluoride include sodium fluoride, potassium fluoride, sodium monofluorophosphate, stannous fluoride, etc. The fluoride may be used alone or in combination of two or more kinds.

[0053] Examples of vitamin preparations include vitamin A such as retinoic acid and β-carotene, vitamin B such as pantothenic acid and its salts, niacin and biotin, vitamin C such as ascorbic acid and its salts and derivatives, vitamin E such as α-tocopherol, folic acid, etc. Vitamin preparations may be used alone or in combination of two or more.

[0054] Examples of herbal extracts include chamomile extract, valerian extract, jujube extract, hop extract, lamenda extract, linden extract, quince extract, goldenrod extract, kumazasa extract, oleander extract, clove extract, ginseng extract, salvia extract, soapberry extract, etc. One type of herbal extract may be used alone, or two or more types may be used in combination.

[0055] The content of the medicinal ingredients such as the above-mentioned moisturizers, antibacterial agents, anti-inflammatory agents, fluorides, vitamins, and herbal extracts in the biofilm decomposition composition of the present invention is not particularly limited as long as the effects of the present invention are achieved, and is, for example, 0.001 to 5.0 mass%, and preferably 0.01 to 1.0 mass%.

[0056] The biofilm decomposition composition of the present invention can be in various forms depending on the subject of application, such as a liquid (e.g., emulsion, solubilized), gel, paste, tablet, effervescent tablet, powder, granule, etc.

[0057] The biofilm decomposition composition of the present invention can be used as various compositions such as oral compositions, cosmetic compositions, detergent compositions, and skin compositions. The oral compositions are compositions intended to decompose biofilms in the oral cavity of humans or animals other than humans (e.g., oral mucosa such as buccal mucosa, tongue, gums, teeth, etc.). Examples of oral compositions include toothpaste, liquid toothpaste, mouthwash, and oral moisturizers.

[0058] Examples of the cosmetic composition include lotions, facial cleansers, makeup removers, milky lotions, creams, eyeliners, beauty essences, ointments, oils, and packs.

[0059] Examples of detergent compositions include detergents for the oral cavity (particularly teeth) such as denture cleaners (liquids, powders, tablets, etc.); detergents for hair such as shampoos, rinses, body shampoos, and conditioners; and general household detergents such as soaps, hand soaps, liquid detergents, kitchen detergents, dishwashing detergents, bathroom detergents, toilet detergents, and pipe detergents.

[0060] [How to break down biofilms] According to another aspect of the present invention, there is provided a method for decomposing a biofilm in a target (hereinafter, also referred to as the "method of the present invention"). The method of the present invention can decompose at least a part of a biofilm by applying the composition for decomposing a biofilm of the present invention to a target on which a biofilm has already formed, for example, by coating. Examples of targets to which the composition for decomposing a biofilm of the present invention can be applied include surfaces of living bodies of humans and non-human animals, such as the oral cavity (e.g., oral mucosa such as buccal mucosa, tongue, gums, teeth, etc.), hair, and skin, as well as surfaces other than living bodies, such as dentures, tableware, kitchens, kitchen utensils, toilets, toilet utensils, bathrooms, bathroom utensils, pipes, furniture, and walls. The amount of the composition for decomposing a biofilm to be applied to a target is not particularly limited as long as the effects of the present invention are achieved, but for example, the amount of the composition for decomposing a biofilm applied to a target is not particularly limited as long as the effects of the present invention are achieved. 2 The amount can be, for example, 0.01 to 2 g, preferably 0.05 to 1.5 g, and more preferably 0.1 to 1 g per unit area. EXAMPLES

[0061] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples. In Tables 1 and 2 below, the numerical values ​​for each component all represent mass % relative to the total mass of the composition for decomposing biofilms.

[0062] [Preparation of biofilm decomposition composition] The components shown in Tables 1 and 2 below were mixed in the amounts shown in the tables to prepare the biofilm decomposition compositions of Examples 1 to 30 and Comparative Examples 1 to 7. Specifically, for the biofilm decomposition compositions of the Examples and Comparative Examples containing sucrose fatty acid esters or polyglyceryl-10 monostearate, the sucrose fatty acid esters or polyglyceryl-10 monostearate were first dispersed in water and dissolved by heating at 60°C. Next, after being cooled to room temperature, other components were mixed to obtain the biofilm decomposition compositions of the Examples and Comparative Examples. For the other Examples and Comparative Examples, the components shown in the tables were mixed in the amounts shown in the tables at room temperature to prepare the test samples of the biofilm decomposition compositions.

[0063] [Table 1]

[0064] [Table 2]

[0065] [Creation of biofilm] Streptococcus mutans JCM5705 strain was pre-cultured in SCD medium (Daigo, Fujifilm Wako Pure Chemical Industries, Ltd.) at 32.5°C for 24 hours to obtain a pre-cultured bacterial solution. The obtained pre-cultured bacterial solution was diluted with water to a concentration of 10 7 The cultured bacteria solution was added to 1% sucrose-containing SCD medium so that the concentration was CFU / mL, and 2 mL of the cultured bacteria solution was seeded on a 24-well plate. The seeded cultured bacteria solution was cultured anaerobically at 37°C for 24 hours to obtain a biofilm.

[0066] [Evaluation of biofilm degradation] Using the biofilm decomposition compositions and biofilms obtained by the above-mentioned procedures, the biofilm decomposition ability of each biofilm decomposition composition was evaluated according to the following procedures.

[0067] First, the biofilm prepared in the 24-well plate was washed twice with 0.01 mol / L phosphate buffered saline (PBS) to remove floating bacteria. Next, 1 mL of each of the biofilm decomposition compositions of the Examples and Comparative Examples was added to the biofilm in the 24-well plate, and ultrasonic irradiation was performed for 30 minutes using an ultrasonic cleaner. Each biofilm decomposition composition was decomposed and removed, and the remaining biofilm was washed twice with 1 mL of PBS, and then stained with 0.35 mL of 0.1% crystal violet solution for 10 minutes. Next, the crystal violet solution was decomposed and removed, washed once with 1 mL of PBS, and then 2 mL of ethanol was added and left to stand for 10 minutes to extract crystal violet from the stained biofilm. The extract was diluted 15 times with ethanol, and the absorbance at 590 nm was measured using a microplate reader. In addition, the absorbance was measured in the same manner using water as a sample to serve as a control. The biofilm survival rate was calculated from the absorbance of each biofilm decomposition composition when the absorbance of the control was set to 100, using the following formula. Biofilm survival rate (%) = absorbance of biofilm decomposition composition / absorbance of control × 100

[0068] The biofilm decomposition ability of each biofilm decomposition composition was evaluated based on the biofilm residual rate according to the following criteria. ⊚: Biofilm residual rate (%) is 20% or less, and biofilm decomposition ability is extremely excellent. ◯: Biofilm residual rate (%) is more than 20% and 50% or less, and the biofilm decomposition ability is excellent. △: Biofilm residual rate (%) is more than 50% and 76% or less, and the biofilm decomposition ability is slightly excellent. ×: Biofilm residual rate (%) is more than 76%, and biofilm decomposition ability is poor.

[0069] From the results shown in Table 1, it is understood that each of the biofilm decomposition compositions of Examples 1 to 30 containing component (A) sucrose fatty acid ester has a superior biofilm decomposition ability compared to the control. In particular, it is understood that each of the biofilm decomposition compositions of Examples 2 to 30 containing component (A) sucrose fatty acid ester and component (B) has a superior biofilm decomposition ability compared to the control. Among them, it is understood that each of the biofilm decomposition compositions of Examples 2 to 18 and 21 to 30 containing zeolite, metaphosphate, polyphosphate, pyrophosphate, phosphate, citric acid, gluconic acid, aspartic acid or glutamic acid as component (B) has a particularly superior biofilm decomposition ability compared to the control. On the other hand, it is understood that each of the biofilm decomposition compositions of Comparative Examples 1 to 7 not containing component (A) sucrose fatty acid ester has an inferior biofilm decomposition ability.

[0070] [Prescription example] The following shows examples of formulations of the biofilm decomposition composition of the present invention prepared by mixing the components described in this specification. The blending amounts are expressed in mass % when the entire biofilm decomposition composition is taken as 100 mass %.

[0071] <Formulation example 1: Gel toothpaste> Sucrose fatty acid ester 1.0 Gluconic acid 1.0 Lactic acid 1.0 Cetylpyridinium chloride 0.05 Hydroxyethyl cellulose 1.0 Sodium hydroxide 0.4 Sodium fluoride 0.1 70% Sorbitol Solution 10 Concentrated glycerin 30 Dipotassium glycyrrhizinate 0.1 Isopropyl methylphenol 0.1 Sodium Lauroyl Sarcosinate 0.1 Polyoxyethylene hydrogenated castor oil 0.5 Glycerin fatty acid ester 0.5 Sodium hyaluronate 0.01 Fragrance 1.0 Remaining purified water Total 100%

[0072] <Formulation Example 2: Toothpaste> Sucrose fatty acid ester 1.0 Sodium polyphosphate 1.0 Sodium metaphosphate 1.0 Sodium pyrophosphate 1.0 Zeolite 1.0 Silica 10 Calcium carbonate 1.0 Sodium dihydrogen phosphate 1.0 Titanium dioxide 0.3 Sodium carboxymethylcellulose 1.5 Cetylpyridinium chloride 0.05 Sodium monofluorophosphate 0.1 Sodium saccharin 0.1 Sodium Lauryl Sulfate 1.5 Sodium Methyl Lauroyl Taurate 0.5 Isopropyl methylphenol 0.1 70% Sorbitol Solution 10 Concentrated glycerin 20 Polyoxyethylene hydrogenated castor oil 1.0 Ethanol 1.0 Fragrance 1.0 Remaining purified water Total 100%

[0073] <Formulation Example 3: Liquid dentifrice> Sucrose fatty acid ester 1.0 Citric Acid 1.0 Glutamic acid 1.25 Cetylpyridinium chloride 0.05 Sodium hydroxide 0.42 Xylitol 1.0 Dipotassium glycyrrhizinate 0.1 Concentrated glycerin 30 Polyoxyethylene hydrogenated castor oil 0.5 Fragrance 1.0 Remaining purified water Total 100%

[0074] <Formulation Example 4: Non-aqueous toothpaste> Sucrose fatty acid ester 1.0 Zeolite 1.0 Sodium pyrophosphate 1.0 Aspartic acid 0.5 Cetylpyridinium chloride 0.05 Silica 10 Hydrated silica 5.0 Titanium dioxide 1.0 Hydroxypropyl cellulose 1.0 Hydroxypropyl methylcellulose 1.0 Polyvinylpyrrolidone 1.0 Sodium fluoride 0.1 Glycyrrhetinic acid 0.1 Sodium saccharin 0.1 Sodium Lauryl Sulfate 1.5 Concentrated glycerin 20 Glycerin fatty acid ester 0.5 Fragrance 1.0 Propylene glycol remainder Total 100%

[0075] It was confirmed that the gel dentifrice, toothpaste, liquid dentifrice and non-aqueous dentifrice of the above formulation examples exhibited good biofilm degradation activity.

Claims

1. A composition for decomposing biofilms, comprising component (A) a sucrose fatty acid ester.

2. The composition for decomposing biofilms according to claim 1, further comprising one or more components selected from the group consisting of silicon compounds, phosphoric acid compounds, hydroxy acid compounds, amino acids, carbonate compounds, and salts thereof, as component (B).

3. The composition for decomposing biofilms according to claim 2 , wherein the silicon compound contains one or more compounds selected from the group consisting of zeolite and silica.

4. The composition for decomposing biofilms according to claim 2, wherein the phosphate compound contains one or more compounds selected from the group consisting of polyphosphoric acid, pyrophosphoric acid, metaphosphoric acid, phosphoric acid, and salts thereof.

5. The composition for decomposing biofilms according to claim 2, wherein the hydroxy acid compound contains one or more selected from the group consisting of citric acid, gluconic acid, and salts thereof.

6. The composition for decomposing biofilms according to claim 2 , wherein the amino acid contains one or more amino acids selected from the group consisting of glutamic acid, aspartic acid, and salts thereof.

7. The composition for decomposing biofilms according to claim 2 , wherein the carbonate compound contains calcium carbonate.

8. The biofilm decomposition composition according to claim 1, wherein the fatty acid group constituting the component (A) has 14 to 18 carbon atoms.

9. The composition for decomposing biofilms according to claim 1, wherein the component (A) contains either one or both of sucrose stearate and sucrose palmitate.

10. The composition for decomposing a biofilm according to claim 1, wherein the HLB value of the component (A) is 10 or more.

11. The composition for decomposing a biofilm according to claim 1, wherein the content of the component (A) is 0.01 to 15 mass% relative to the total mass of the composition for decomposing a biofilm.

12. The composition for decomposing a biofilm according to claim 2, wherein the content of the component (B) is 0.01 to 15 mass% relative to the total mass of the composition for decomposing a biofilm.

13. The composition for decomposing biofilms according to claim 1, which is for use in the oral cavity.

14. The biofilm decomposition composition according to claim 1, which is a dentifrice, a mouthwash or an oral moisturizer.

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