AREG gene expression promoter

Abstract

To provide an AREG gene expression promoter.SOLUTION: Provided is an AREG (amphiregulin) gene expression promoter containing at least one selected from the group consisting of Glycyrrhiza leaf extract and Belamcanda chinensis extract.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to an AREG gene expression promoter and a product for promoting AREG gene expression.

Background Art

[0002] AREG (Amphiregulin) is a protein belonging to the epidermal growth factor family and is encoded by the AREG gene.

[0003] Although it is known that the gene expression level of the AREG gene is modulated after ultraviolet exposure (Patent Document 1), the control of AREG gene expression has not been sufficiently studied.

[0004] In order to suppress the decline in the quality of life and live longer and healthier lives, external use products and oral products that suppress AREG gene expression are desired.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0006] An object of the present invention is to provide an AREG gene expression promoter containing one or more selected from the group consisting of licorice leaf extract and forsythia extract.

Means for Solving the Problems

[0007] The present inventors have surprisingly found that a composition containing one or more selected from the group consisting of licorice leaf extract and forsythia extract promotes the expression of the AREG gene and has an AREG gene expression promoting effect. The present invention is based on this finding and provides the following inventions.

[0008] [1] An AREG gene expression promoter containing, as an active ingredient, one or more selected from the group consisting of licorice leaf extract and forsythia extract. [2] An external composition for promoting AREG gene expression containing, as an active ingredient, one or more selected from the group consisting of licorice leaf extract and forsythia extract. [3] A food or drink composition for promoting AREG gene expression containing, as an active ingredient, one or more selected from the group consisting of licorice leaf extract and forsythia extract.

Effect of the Invention

[0009] Since the AREG gene expression promoter of the present invention contains one or more selected from the group consisting of licorice leaf extract and forsythia extract, it can promote the expression of the AREG gene.

Brief Description of the Drawings

[0010]

Figure 1

Figure 2

Modes for Carrying Out the Invention

[0011] Hereinafter, the modes for carrying out the present invention will be described in detail. However, the present invention is not limited to the following embodiments.

[0012] 〔1. AREG gene expression promoter〕 The AREG gene expression promoter according to this embodiment contains one or more selected from the group consisting of licorice leaf extract and forsythia extract.

[0013] Licorice means a plant of the legume family. For the licorice leaf extract of the present invention, an extract of the leaves is preferred. The extraction method and extraction solvent of licorice leaves are not particularly limited, and known techniques can be used. The extract may be used as it is, or a powder or granule obtained by drying the extract may be used, or a decomposed product obtained by treating the extract using a known technique such as enzymatic decomposition may be used. Commercially available products may also be used.

[0014] Iris means the leaves of a plant belonging to the iris family. For the iris extract of the present invention, any part may be used, or one or more parts may be selected and used. In particular, an extract of the rhizome is preferred. The extraction method and extraction solvent of iris are not particularly limited, and known techniques can be used. The extract may be used as it is, or a powder or granule obtained by drying the extract may be used, or a decomposed product obtained by treating the extract using a known technique such as enzymatic decomposition may be used. Commercially available products may also be used.

[0015] When obtaining an extract of licorice leaves and / or iris using a solvent, any commonly used solvent may be used. For example, an extract obtained by extracting with one or more polar solvents such as ethanol and water, a powder obtained by drying the extract, a pulverized product, extract, dried product, etc. of these, purified by distribution between a column and a solution to enhance the active ingredient, etc. can be used. Commercially available products may also be used.

[0016] The AREG gene expression promoter of the present invention has an effect of promoting the expression of the AREG gene, and effects such as promoting the expression of the AREG gene are expected. When the gene expression of AREG, which is an epidermal growth factor, is promoted, the proliferation of basal cells is promoted and epidermal turnover is promoted. Since the expression of AREG decreases with aging, a preventive and / or ameliorating effect on the decrease in epidermal turnover associated with aging is expected.

[0017] In the AREG gene expression promoter according to this embodiment, the contents of the licorice leaf extract and the forsythia extract are not particularly limited as long as they have the AREG gene expression promoting effect, and are appropriately set according to the types and contents of other formulation components, the dosage form of the AREG gene expression promoter, and the like. From the viewpoint of more significantly exhibiting the effects of the present invention, for example, it is preferably 0.00001 to 5 w / v%, more preferably 0.00002 to 3 w / v%, still more preferably 0.00005 to 1 w / v%, and particularly preferably 0.0001 to 1 w / v%. The dosage for human administration is preferably 0.001 to 2000 mg per day, more preferably 0.01 to 1000 mg, still more preferably 0.1 to 500 mg, and particularly preferably 1 to 300 mg.

[0018] The AREG gene expression promoter according to this embodiment may further contain excipients, thickeners, binders, disintegrants, dispersants, surfactants, suspending agents, emulsifiers, stabilizers, gelling agents, sweeteners, acidulants, umami agents, saltiness agents, bitterness agents, coloring agents, ultraviolet ray preventives, antioxidants, preservatives, antibacterial agents, pH adjusters, buffers, chelating agents, vitamins, fragrances, minerals and other additives, amino acids or their salts, saccharides, humectants, organic acids or their salts, dietary fibers, animal tissue extracts, etc. These can be appropriately selected from known ones and used, and can be manufactured using conventional methods.

[0019] The dosage form of the AREG gene expression promoter according to this embodiment can be used without being limited to specific products and properties, and can be appropriately prepared by known methods in any dosage form such as liquid, gel, cream, paste, solid, aerosol or patch. Examples of liquid forms include lotion, emulsion, essence, oral liquid, syrup, etc. Examples of solid forms include stick, tablet, chewable, granule, powder, capsule, ointment, etc. In terms of the administration form, although it does not matter whether it is an external preparation or an internal preparation, from the viewpoint of achieving the effects of the present invention, it is preferably an external preparation. The external preparation is not limited, whether it is a pharmaceutical or a cosmetic. These can be manufactured using conventional methods.

[0020] [[External preparation for promoting AREG gene expression]] The external preparation according to this embodiment has an action of promoting the expression of the AREG gene. Therefore, as one embodiment of the present invention, there is provided an external preparation for promoting AREG gene expression containing one or more selected from the group consisting of licorice leaf extract and forsythia extract.

[0021] In addition, regarding the content of one or more selected from the group consisting of licorice leaf extract and forsythia extract in this embodiment, the types and contents of other components, the dosage form and use of the external preparation, etc., are as described in [1. AREG gene expression promoter].

[0022] [[Food and drink products for promoting AREG gene expression]] The food and drink product according to this embodiment has an action of promoting the proliferation of satellite cells. Therefore, as one embodiment of the present invention, there is provided a food and drink product for promoting AREG gene expression containing one or more selected from the group consisting of licorice leaf extract and forsythia extract.

[0023] In addition, regarding the content of one or more selected from the group consisting of licorice leaf extract and forsythia extract in this embodiment, the types and contents of other components, the dosage form and use of the external preparation, etc., are as described in [1. AREG gene expression promoter]. [[Examples]]

[0024] Hereinafter, the present invention will be specifically described based on test examples, but the present invention is not limited thereto. In addition, the blending amounts in the following test examples, etc. indicate mass% unless otherwise specified. The test conditions such as the culture conditions in each test example were made the same except for the difference in the plant extracts to be added, unless otherwise specified. In addition, for each test, a control without adding the plant extract was provided to accurately evaluate the effect of the plant extract of the present invention.

[0025] [[Test Example 1: Analysis of AREG gene expression]] The AREG gene expression analysis was performed by the following method. Normal human dermal fibroblasts (Kurabo Industries Ltd.) were seeded in a 48-well plate at a density of 15,000 cells / 250 μL, and all of the attached LifeFactors in HuMedia-KG2 (KURABO, KK-2150S) were added and used. The cells were cultured at 37°C under 5% carbon dioxide for 24 hours. After aspirating and removing the medium, the Folium Isatidis extract and the Folium Mori extract diluted with HuMedia-KG2 (KURABO, KK-2150S) were added to the 48-well plate at 250 μL / well (Test solution 1). The final concentration of the Folium Isatidis extract was 0.01%, and the final concentration of the Folium Mori extract was 0.001%. The cells were cultured at 37°C under 5% carbon dioxide for 6 hours, and then RNA was collected. Cells cultured for 6 hours with only the medium without the Folium Isatidis extract and the Folium Mori extract were used as a control. Next, the AREG gene expression was measured using the real-time PCR method. GAPDH was used as the endogenous control for gene expression. The AREG gene expression of Test solution 1 was calculated with the AREG gene expression of the control set to 100. The results are shown in Figure 1.

[0026] As shown in Figure 1, in Test solution 1 containing the Folium Isatidis extract and the Folium Mori extract, the AREG gene expression level increased compared to the control. The Folium Isatidis extract and the Folium Mori extract promoted the expression of the AREG gene.

[0027] 〔Test Example 2: Gene Expression Analysis of 3D-Cultured Skin by Plasma Irradiation〕 The gene expression analysis of 3D-cultured skin by plasma irradiation was performed by the following method. A three-dimensional cultured skin model (KURARAY CO., LTD., EFT-400) composed of human normal keratinocytes and fibroblasts was cultured for 24 hours at 37°C in an environment of 5% carbon dioxide and 95% air using a medium for EFT-400 (KURARAY CO., LTD., EFT-400ASY). After culturing, the surface of the epidermis was irradiated once with a nitrogen plasma laser (Neogen PSR) at 0.8 J / pulse, and further cultured for 6 hours. Those not irradiated with the nitrogen plasma laser were used as controls. After culturing, RNA was recovered using the Rneasy Fibrous Tissue Mini kit (Qiagen). Next, the expression of the AREG gene was measured using the real-time PCR method. GAPDH was used as the endogenous control for gene expression. When the AREG gene expression of the control was set to 100, the AREG gene expression when irradiated with the nitrogen plasma laser was calculated. The results are shown in Figure 2.

[0028] As shown in Figure 2, the expression of the AREG gene increased by irradiation with the nitrogen plasma laser. The expression of the AREG gene can be increased by irradiation with the nitrogen plasma laser.

Claims

【Claim 1】 An AREG gene expression promoter containing, as an active ingredient, one or more selected from the group consisting of licorice leaf extract and forsythia extract.

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