Topical composition for skin

Abstract

To provide a topical composition for skin that can suppress reduction in activity of proteolytic enzymes and lipolytic enzymes.SOLUTION: A topical composition for skin comprising: (A) a proteolytic enzyme and / or a lipolytic enzyme, and (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium robertianum extract, hyaluronic acid, and a salt thereof.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a composition for external application to the skin, and more particularly to a composition for external application to the skin containing a protease or a lipase. [Background technology]

[0002] Various topical skin compositions containing protease or lipase have been proposed. For example, Patent Documents 1 and 2 disclose a facial cleansing powder containing protease and a facial cleansing cream containing protease. These skin cleansers are known to be effective in removing skin dirt derived from keratin, sweat gland secretions, etc., and are therefore useful in eliminating, for example, darkened pores, clogged keratin plugs, roughness, etc. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Japanese Patent Application Laid-Open No. 2009-256211 [Patent Document 2] Japanese Patent Publication No. 2020-011909 Summary of the Invention [Problem to be solved by the invention]

[0004] However, since the enzymatic activity of proteases and lipolytic enzymes generally tends to decrease over time in the presence of water, there is a concern that the enzymatic activity may be lost due to external factors, for example. Specifically, there is a concern that the enzymatic activity may decrease over time due to the presence of moisture in the manufacturing environment of cosmetics containing proteases, the distribution environment in the market, and the usage environment of consumers, so there are limitations on the handling and packaging form of the products.

[0005] The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a composition for external use on skin that can suppress the decline in the enzymatic activity of proteases and lipolytic enzymes. [Means for solving the problem]

[0006] In light of the above circumstances, the inventors have conducted extensive research and have unexpectedly discovered that the decrease in activity of proteolytic enzymes and lipolytic enzymes can be suppressed by using one or more components selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts.

[0007] That is, the present invention provides the following [1] to [7]. [1] (A) a proteolytic enzyme and / or a lipolytic enzyme, (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts; A composition for external use on the skin comprising: [2] The mass of the component (B) per unit of the component (A) (the mass (g) of the component (B) contained in one gram of the composition for topical skin / the number of units (U) of the component (A)) is 1.0 × 10 -10 ~1.0×10 -1 [1] The topical skin composition described in [1]. [3] The mass of (C) water per unit of the (A) component (mass of (C) water (g) / number of units of the (A) component (U) contained in one unit of the topical skin composition) is 0 to 6.0 × 10 -2 The composition for external use on skin according to [1] or [2], [4] The mass of (C) water per unit of the (A) component (the mass of (C) water (g) contained in one gram of the composition for external use on skin / the number of units of the (A) component (U)) is 7.5 × 10 -4 ~6.0×10 -2 The composition for external use on skin according to [1] or [2], [5] The composition for external use on skin according to any one of [1] to [4], wherein the component (B) is one or more selected from the group consisting of inulin, Ascophyllum nodosum extract, and Geranium globulus extract. [6] A stabilizer for proteolytic enzymes and / or lipid-degrading enzymes in a topical skin composition, which contains as active ingredients one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts. [7] A method for stabilizing the enzymatic activity of (A) a protease and / or a lipolytic enzyme by causing (A) a protease and / or a lipolytic enzyme to coexist with (B) one or more members selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts in a topical skin composition. [Effects of the Invention]

[0008] The topical skin composition of the present invention contains, in addition to protease and lipolytic enzyme, one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts, and therefore can suppress a decrease in the enzymatic activity of protease and lipolytic enzyme. [Brief explanation of the drawings]

[0009] [Figure 1] FIG. 1 shows the results of enzyme activity tests in the Examples. DETAILED DESCRIPTION OF THE INVENTION

[0010] The present invention will be described in detail below with reference to exemplary embodiments, but the present invention is not limited to the following exemplary embodiments.

[0011] In this specification, when the expression "X to Y" (X and Y are any numbers) is used, unless otherwise specified, it includes the meaning of "X or more and Y or less," as well as "preferably larger than X" or "preferably smaller than Y." Furthermore, in this specification, unless otherwise specified, "α and / or β" (α and β are optional structures or components) means three combinations: "α only," "β only," and "both α and β." In addition, in the present specification, with respect to the numerical ranges described in stages, the upper or lower limit of a certain stage can be arbitrarily combined with the upper or lower limit of another stage. Furthermore, in the numerical ranges described in this specification, the upper or lower limit of the numerical range can also be replaced with the values ​​shown in the examples or formulation examples.

[0012] <Skin external composition> A topical skin composition according to one embodiment of the present invention (hereinafter sometimes referred to as "the topical skin composition") contains (A) a protease and / or a lipolytic enzyme, and (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts. The use of component (B) can suppress a decrease in enzyme activity. Each component will be described below.

[0013] [Component (A)] The present topical skin composition contains a protease and / or a lipase for the purpose of effectively removing, for example, proteinaceous dirt from the skin, aged keratin, and sebum and its oxidized substances accumulated in hair roots. These may be used alone or in combination of two or more. Among these, it is preferable to contain a protease in order to significantly achieve the effects of the present invention.

[0014] The protease may be any of acidic, neutral, or alkaline proteases, and may be any of animal-, plant-, or microbial-derived proteases. Specific examples of proteases include plant-derived proteases such as papain, chymopapain, bromelain, ficin, and pepsin; animal-derived proteases such as trypsin, chymotrypsin, and pancreatin; and microbial proteases produced by microorganisms such as bacteria. Microbial proteases include proteases produced by microorganisms of the genus Bacillus, Paenibacillus, Geobacillus, Aspergillus, Rhizopus, Rhizomucor, and Streptomyces. These may be used alone or in combination of two or more.

[0015] Among the above proteases, proteases derived from microorganisms and proteases derived from plants are preferred, and proteases produced by microorganisms of the genus Bacillus are more preferred, from the viewpoint of significantly achieving the effects of the present invention.

[0016] The lipolytic enzyme may be any of acidic, neutral, or alkaline lipolytic enzymes, and may be any of animal-, plant-, or microbial-derived lipolytic enzymes. Specific examples of lipolytic enzymes include lipase, phospholipase, and phytase. Examples of microbial-derived lipolytic enzymes include lipolytic enzymes produced by microorganisms such as Rhizopus, Aspergillus, Mucor, Geotrichum, Candida, Pseudomonas, Penicillium, and Chromobacterium. These may be used alone or in combination of two or more. Among these, microbial-derived lipolytic enzymes are preferred in terms of significantly achieving the effects of the present invention.

[0017] Commercially available products of component (A) include, for example, "Protease [e.g., Protease CL-15]," "Biophrase (registered trademark) [e.g., Bioprase XL-416F]" (all manufactured by Nagase ChemteX Corporation), "Protease N," "Protease S," and "Papain W-40" (all manufactured by Amano Pharmaceutical Co., Ltd.), "Biosork" (manufactured by Yamato Chemical Industry Co., Ltd.), "Alcalase (registered trademark)," "Esperase (registered trademark)," "Savinase (registered trademark)," "Durazyme (registered trademark)," and "Subtilisin A" (all manufactured by Novozymes Japan), and "Lilipase (registered trademark) [e.g., Lilipase A-10D]" (manufactured by Nagase ChemteX Corporation).

[0018] The total content of component (A) can be appropriately set depending on the type and amount of other components, dosage form, etc., and is not limited. However, from the viewpoint of significantly achieving the effects of the present invention, the component (A) is preferably formulated in an amount of 1 to 1000 U / g, more preferably 5 to 800 U / g, even more preferably 10 to 600 U / g, and particularly preferably 15 to 200 U / g in the topical skin composition. The "U (unit)" in the unit "U / g" indicates the activity of the enzyme, and one unit of protease is defined as the amount of enzyme (1 micromole per minute) that can convert 1 micromole (μmol) of substrate per minute under optimal conditions (a temperature of 30°C and an acidity at which the chemical reaction proceeds most rapidly). One unit of lipase is defined as the amount of enzyme (1 micromole per minute) that liberates an acid equivalent to 1 μmol of oleic acid when olive oil is reacted with an enzyme solution at 37°C for 60 minutes, the reaction is stopped, and the resulting fatty acids are measured by titration.

[0019] Furthermore, the total content of component (A) can be set appropriately depending on its enzyme activity (U / g), the types and amounts of other components, the dosage form, etc., and is not limited to a specific amount, but is preferably 0.001 to 4 mass%, more preferably 0.005 to 3.2 mass%, even more preferably 0.01 to 2.4 mass%, and particularly preferably 0.01 to 0.8 mass%, relative to the total amount of the topical skin composition (100 mass%).

[0020] Component (A) may be either an immobilized enzyme, i.e., an enzyme immobilized by a method such as immobilization on a carrier, or an unimmobilized enzyme, i.e., an enzyme not immobilized by a method such as immobilization on a carrier. Carriers onto which enzymes are immobilized include known carriers typically used for enzyme immobilization. Examples include organic polymer compounds such as various ion exchange resins, and inorganic porous materials such as ceramics. Various known methods can be used to immobilize enzymes. Examples include entrapment immobilization, inorganic carrier covalent bonding, organic carrier covalent bonding, and physical adsorption. Among these, unimmobilized enzymes are preferred from the viewpoint of ease of formulation.

[0021] [(B) component] This topical skin composition contains (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts in order to suppress a decrease in the enzymatic activity of proteolytic enzymes and lipolytic enzymes. The component (B) may contain one or more components selected from the group consisting of the above components, and any number of components, for example, three or more components, may be used in combination.

[0022] The total content of component (B) can be appropriately set depending on the types and amounts of other components, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is preferably 0.00001 to 20% by mass, more preferably 0.0001 to 10% by mass, even more preferably 0.0001 to 5% by mass, and even more preferably 0.0001 to 3% by mass relative to the total amount of the topical skin composition (100% by mass). From another viewpoint, it is preferably 0.01 to 20% by mass, preferably 0.1 to 10% by mass, and even more preferably 0.5 to 5% by mass.

[0023] The mass of component (B) per unit of component (A) (mass (g) of component (B) contained per unit g of the composition for topical skin / number of units (U) of component (A)) can be appropriately set depending on the type and amount of other components, dosage form, etc., and is not limited thereto. From the viewpoint of significantly exhibiting the effects of the present invention, it is preferred that the mass of component (B) per unit g of the composition for topical skin be 1.0 × 10 -10 ~1.0×10 -1 is preferable, and more preferably 5.0 × 10 -9 ~7.0×10 -3 , and more preferably 5.0 × 10 -8 ~4.0×10 -3 is.

[0024] [Inulin] An example of an embodiment of the present external skin composition includes an embodiment containing one or more enzymes selected from the group consisting of protease and lipolytic enzyme, and at least inulin as component (B). Inulin is a polysaccharide in which multiple fructose molecules are bonded to glucose. Inulin is a fructan polymer represented by GFn (G: glucose, F: fructose, n: number of fructose molecules (n≧2)), and has a structure in which two or more D-fructose molecules are dehydrated and polymerized on the fructose side of sucrose via a β-(2→1) bond. These can be used alone or in combination of two or more types.

[0025] The inulin is not particularly limited, but may be obtained by extraction and purification from plants such as chicory, Jerusalem artichoke, garlic, burdock, onion, sugarcane, etc., or may be synthesized. Among these, sugarcane-derived inulin is a preferred example.

[0026] The average degree of polymerization of inulin is not particularly limited, but is preferably 5-50, more preferably 7-40, and even more preferably 10-30, for example. The distribution of the degree of polymerization of inulin is not particularly limited, but is preferably in the range of 3-80, more preferably in the range of 5-70, and even more preferably in the range of 5-60, for example.

[0027] A preferred example of inulin is inulin (aggregate) having an average degree of polymerization of 13 to 23 and containing inulin having a degree of polymerization of 5 to 29 in an amount of 95 mass % or more.

[0028] The average degree of polymerization and distribution of inulin can be measured by known methods, for example, by chromatography. Specifically, the top of the peak in each analytical result obtained by HPLC analysis can be used as the average degree of polymerization.

[0029] The total content of inulin is appropriately set within the range exemplified above for the total content of component (B). Specifically, the total content of inulin can be appropriately set depending on the types and amounts of other components, dosage form, etc., and is not limited thereto. However, from the viewpoint of significantly achieving the effects of the present invention, the total content of inulin is preferably 0.01 to 20% by mass, more preferably 0.05 to 10% by mass, and even more preferably 0.1 to 5% by mass relative to the total amount (100% by mass) of the topical skin composition.

[0030] From the viewpoint of significantly achieving the effects of the present invention, the mass of inulin per unit of component (A) (mass of inulin (g) contained in unit g of the composition for external use on skin / number of units of component (A) (U)) is 1.0 × 10-7 ~1.0×10 -1 is preferable, and more preferably 5.0 × 10 -6 ~7.0×10 -3 , and more preferably 5.0 × 10 -6 ~4.0×10 -3 is.

[0031] [Inositol] An example of an embodiment of the present external skin composition includes one or more enzymes selected from the group consisting of proteases and lipolytic enzymes, and at least inositol as component (B). Inositol is a cyclic hexahydric alcohol in which one hydrogen atom on each carbon atom of cyclohexane is replaced with a hydroxy group. Inositol may be any stereoisomer, including cis-inositol, epi-inositol, allo-inositol, myo-inositol, muco-inositol, neo-inositol, chiro-inositol (both D- and L-forms exist), and scyllo-inositol. Among these, myo-inositol is preferred. These may be used alone or in combination.

[0032] The inositol is not particularly limited, and it may be obtained by extraction from rice bran, chemical synthesis, fermentation, etc. Among these, rice bran-derived inositol extracted from rice bran is a suitable example.

[0033] The total content of inositol is appropriately set within the range exemplified above for the total content of component (B). Specifically, the total content of inositol can be appropriately set depending on the types and amounts of other components, dosage form, etc., and is not limited thereto. From the viewpoint of significantly achieving the effects of the present invention, the total content of inositol is preferably 0.01 to 14% by mass, more preferably 0.05 to 10% by mass, and even more preferably 0.1 to 5% by mass relative to the total amount (100% by mass) of the topical skin composition.

[0034] The mass of inositol per unit of component (A) (mass of inositol (g) contained in unit g of topical skin composition / number of units of component (A) (U)) is 1.0 × 10 -7 ~1.0×10 -2 is preferable, and more preferably 1.0 × 10 -6 ~7.0×10 -3 , and more preferably 5.0 × 10 -6 ~4.0×10 -3 is.

[0035] [Seaweed extract] One embodiment of the present topical skin composition includes one or more enzymes selected from the group consisting of proteases and lipolytic enzymes, and at least a seaweed extract as component (B). The seaweed extract is an extract extracted from seaweed such as brown algae, green algae, and red algae. These may be used alone or in combination of two or more.

[0036] Examples of brown algae include Ascophyllum nodosum, kelp (Kagome kelp, Laminaria japonica, Lisilis kelp, Narrow-skinned kelp, Mitsuishi kelp), giant kelp, wakame or blue wakame, Mozuku, Undaria pinnatifida, Hijiki, Fucus fucus, sea fans, Pale-striped sea fans, Yellow-striped sea fans, Red-striped sea fans, Kona sea fans, Okinawa fan, Pale-striped sea fans, and Etsuki sea fans.

[0037] Examples of green algae include Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidea, Green laver, Ulva, and Ulva pertusa.

[0038] Examples of red algae include Hijirimen, Aster or Gelidium, Hirakusa, Onikusa, Obakusa, Kataobakusa, Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusa Eucheuma, Eucheuma, Byakushin Eucheuma, Tsunomata, Oobatonomata, Tochaka or Yahazutsunomata, Ezotsunomata, Togetsunomata, Hirakotoji, Kotojitsunomata, Ibotsunomata, Marubatonomata, Hirakotoji, Suginori, Shikinori, Kainori, Yareusubanori, Kagiusubanori, Sujiusubanori, Haiusubanori, and Akamomijinanori.

[0039] Among these seaweed extracts, brown algae seaweed extracts are preferred, and Ascophyllum nodosum extract and Kjellmannia crassipes extract are more preferred.

[0040] The seaweed extract can be extracted by a commonly used method. That is, the seaweed extract can be a crude extract extracted from the whole plant or a necessary part, or a product obtained by further purification, concentration, or synthesis. Commercially available products can also be used.

[0041] The extraction method for seaweed extract can be any known method, and is not particularly limited, and examples thereof include hot water extraction, ultrasonic extraction, filtration, reflux extraction, and solvent extraction. The solvent is also not particularly limited, and examples thereof include water; aqueous solutions of inorganic salts such as sodium chloride, potassium chloride, magnesium chloride, and ammonium carbonate; buffer solutions such as phosphate buffer, acetate buffer, Tris-HCl buffer, carbonate buffer, and borate buffer; aqueous solutions of inorganic acids such as hydrochloric acid, carbonic acid, sulfuric acid, nitric acid, and phosphoric acid; aqueous solutions of organic acids such as acetic acid, citric acid, lactic acid, succinic acid, ascorbic acid, fumaric acid, and malic acid; aqueous solutions of surfactants such as saponin and lecithin; lower alcohols such as methanol, ethanol, propanol, and butanol; and ketones such as acetone and ethyl methyl ketone. Examples of hydrophilic or polar solvents include: Other examples of the solvent include hydrophobic solvents such as hydrocarbons such as propane, butane, hexane, and cyclohexane; glycerin; ethers such as diethyl ether; glycols such as propylene glycol; halogenated hydrocarbons such as dichloromethane, 1,1,1,2-tetrafluoroethane, and 1,1,2-trichloroethene; and acetates such as ethyl acetate and methyl acetate. The solvents can be used alone or in combination of two or more. Specifically, for example, a solvent is added to the part of the seaweed to be extracted or its dried product, and the product is soaked for several days to several hours under appropriate temperature conditions, cooled to room temperature, and then insoluble matter and / or the solvent are removed, and the product can be extracted by a method such as fractionation and purification using column chromatography or the like.

[0042] The seaweed extract may be in liquid form, but if necessary, it may be in concentrated liquid, semi-solid, solid, or powder form by reducing or removing the liquid content through drying processes such as vacuum drying, freeze drying, or spray drying.

[0043] The total content of the seaweed extract is appropriately set within the range exemplified above for the total content of component (B). Specifically, the total content of the seaweed extract can be appropriately set depending on the types and amounts of other components, dosage form, etc., and is not limited thereto. However, from the viewpoint of significantly achieving the effects of the present invention, the total content of the seaweed extract is preferably 0.0001 to 5% by mass, more preferably 0.0002 to 3% by mass, and even more preferably 0.0004 to 2% by mass relative to the total amount (100% by mass) of the topical skin composition.

[0044] The mass of seaweed extract per unit of component (A) (mass of seaweed extract (g) contained in unit g of topical skin composition / number of units of component (A) (U)) is 1.0 × 10 -9 ~7.0×10 -3 is preferable, and more preferably 1.0 × 10 -8 ~4.0×10 -3 , and more preferably 1.0 × 10 -7 ~2.0×10 -3 is.

[0045] In addition, when various extracts are formulated in the present topical skin composition in the form of a solution, the content of the various extracts in the present topical skin composition means the pure content of the extract, and is calculated by multiplying the liquid volume of the solution by the concentration of the extract in that solution.

[0046] [Geranium globulus extract] An example of an embodiment of the present topical skin composition includes one or more enzymes selected from the group consisting of proteolytic enzymes and lipolytic enzymes, and at least Geranium globulus extract as component (B). Geranium robertianum extract is an extract of Geranium robertianum, a plant of the Geraniaceae family.

[0047] The Geranium globulus extract can be extracted by a commonly used method, specifically the same as that described for the seaweed extract.

[0048] The total content of Geranium robertii extract is appropriately set within the range exemplified for the total content of component (B) above. Specifically, the total content of Geranium robertii extract can be appropriately set depending on the types and amounts of other components, dosage form, etc., and is not limited thereto. However, from the viewpoint of significantly achieving the effects of the present invention, the total content of Geranium robertii extract is preferably 0.0001 to 1% by mass, more preferably 0.0001 to 0.5% by mass, even more preferably 0.0001 to 0.2% by mass, particularly preferably 0.0001 to 0.1% by mass, and especially preferably 0.0001 to 0.05% by mass relative to the total amount (100% by mass) of the topical skin composition.

[0049] From the viewpoint of significantly achieving the effects of the present invention, the mass of Geranium globulus extract per unit of component (A) (mass of Geranium globulus extract (g) contained per unit g of topical skin composition / number of units of component (A) (U)) is 1.0 × 10 -10 ~7.0×10 -4 is preferable, and more preferably 1.0 × 10 -9 ~3.0×10 -4 , and more preferably 5.0 × 10 -9 ~2.0×10 -4 , particularly preferably 1.0 × 10 -8 ~7.0×10 -5 , particularly preferably 5.0 × 10 -8 ~3.0×10 -5 is.

[0050] [Hyaluronic acid and its salts] An example of an embodiment of the present topical skin composition includes one or more enzymes selected from the group consisting of proteases and lipolytic enzymes, and at least hyaluronic acid and / or a salt thereof as component (B). Hyaluronic acid is a type of acidic mucopolysaccharide, a polysaccharide containing a disaccharide of glucuronic acid and N-acetylglucosamine as a structural unit. Hyaluronic acid can be extracted and recovered from animal tissues such as rooster comb, shark skin, umbilical cord, eyeball, skin, cartilage, etc., or from cultures of hyaluronic acid-producing microorganisms such as Streptococcus, animal cells, and plant cells.

[0051] The salt of hyaluronic acid can be any pharmaceutically or physiologically acceptable salt, for example, can be the salt with organic base (such as organic amine salts such as methylamine salt, triethylamine salt, triethanolamine salt, morpholine salt, piperazine salt, pyrrolidine salt, tripyridine salt, picoline salt), the salt with inorganic base (such as ammonium salt; alkali metal salts such as sodium salt, potassium salt, alkaline earth metal salts such as calcium salt, magnesium salt, zinc salt, aluminum salt, etc.).Among these salts, the salt with inorganic base is preferred, alkali metal salt is more preferred, sodium salt, potassium salt is even more preferred, and sodium salt is particularly preferred.Hyaluronic acid and its salt can be used alone or in combination of two or more kinds.

[0052] From the viewpoint of significantly achieving the effects of the present invention, the weight average molecular weight of hyaluronic acid or a salt thereof is preferably 400,000 to 3,000,000, more preferably 500,000 to 2,700,000, even more preferably 600,000 to 2,200,000, and particularly preferably 1,100,000 to 1,600,000.

[0053] The weight-average molecular weight means a viscosity-average molecular weight, and can be determined by a known measurement method. Specifically, hyaluronic acid or its salt (dried product) is dissolved in a 0.2 M sodium chloride solution, and the intrinsic viscosity (η) at 30±0°C is determined using an Ubbelohde viscometer, and the intrinsic viscosity is calculated using the Laurent formula (η (intrinsic viscosity) = 3.6 × 10 -4 M 0.78 The viscosity average molecular weight is calculated based on the following: (where M is the viscosity average molecular weight). The intrinsic viscosity (η) is measured according to the 18th Edition of the Japanese Pharmacopoeia, General Test Methods, Viscosity Measurement Method, Method 1: Capillary Viscometer Method.

[0054] Commercially available products of hyaluronic acid or its salts include sodium hyaluronate HA12N (trade name) (manufactured by Shiseido Co., Ltd.; weight-average molecular weight 1.1 million to 1.6 million), hyaluronic acid IW120B (trade name) (manufactured by Speranexus Co., Ltd.; weight-average molecular weight 1.1 million to 1.6 million), hyaluronic acid FCH-150 (trade name) (manufactured by Kikkoman Biochemifa Co., Ltd.; weight-average molecular weight 1.4 million to 1.8 million), and sodium biohyaluronate SZE (trade name) (manufactured by Shiseido Co., Ltd.; weight-average molecular weight 1.1 million to 1.6 million). 00,000), hyaluronic acid FCH-120 (trade name) (manufactured by Kikkoman Biochemifa Corporation; weight-average molecular weight 1,000,000 to 1,400,000), hyaluronic acid HA-LQ (trade name) (manufactured by Kewpie Corporation; weight-average molecular weight 850,000 to 1,600,000), hyaluronic acid FCH-80 (trade name) (manufactured by Kikkoman Biochemifa Corporation; weight-average molecular weight 600,000 to 1,000,000), hyaluronic acid FCH-60 (trade name) (manufactured by Kikkoman Biochemifa Corporation; weight-average molecular weight 500,000 to 700,000), and the like.

[0055] The total content of hyaluronic acid and / or its salts can be appropriately set within the range exemplified as the total content of the above-mentioned component (B).Specifically, the total content of hyaluronic acid and / or its salts can be appropriately set according to the type and amount of other components, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is preferably 0.001-10% by mass, more preferably 0.005-5% by mass, even more preferably 0.01-1% by mass, particularly preferably 0.01-0.5% by mass relative to the total amount (100% by mass) of the external skin composition.

[0056] From the viewpoint of significantly exhibiting the effects of the present invention, the mass of hyaluronic acid and / or a salt thereof per unit of component (A) (mass (g) of hyaluronic acid and / or a salt thereof contained per unit g of the composition for external use on skin / number of units of component (A) (U)) is 5.0 × 10 -8 ~7.0×10 -3 is preferable, and more preferably 2.5 × 10 -7 ~4.0×10 -3 , and more preferably 5.0 × 10 -7 ~7.0×10 -4 , particularly preferably 5.0 × 10 -7~4.0×10 -4 is.

[0057] [(C) component] In addition to components (A) and (B), the present composition for external use on skin may further contain water (C). Examples of component (C) include tap water, ion-exchanged water, distilled water, and reverse osmosis water. The total content of component (C) can be appropriately set depending on the type and amount of other components, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it may be, for example, 15 to 50% by mass, preferably 20 to 45% by mass, and more preferably 20 to 40% by mass relative to the total amount (100% by mass) of the topical skin composition in one embodiment. In another embodiment, it may be 50 to 90% by mass, preferably 55 to 85% by mass, and more preferably 60 to 75% by mass. In yet another embodiment, it may be 0 to 5% by mass, preferably 0 to 0.5% by mass, and more preferably 0 to 0.05% by mass.

[0058] In one embodiment, the mass of (C) water per unit of (A) component (the mass (g) of (C) water contained per gram of the composition for external use on skin / the number of units (U) of (A) component) is 7.5 × 10 -4 ~4.0×10 -2 is preferable, and more preferably 1.0 × 10 -3 ~3.0×10 -2 , and more preferably 1.0 × 10 -3 ~2.0×10 -2 In another embodiment, it may be 1.0×10 -3 ~6.0×10 -2 is preferable, and more preferably 1.0 × 10 -3 ~5.5×10 -2 , and more preferably 1.0 × 10 -3 ~5.0×10 -2 In still another embodiment, the value may be 0 to 4.0×10 -3 is preferable, and more preferably 0 to 4.0 × 10 -4 , and more preferably 0 to 4.0 × 10 -5 may be.

[0059] [Other ingredients] The present topical skin composition can contain other ingredients in addition to components (A) to (C) as long as the effects of the present invention are not impaired. Examples of ingredients that can be included include, but are not limited to, bases or carriers, surfactants, thickeners, antiseptics, pH adjusters, antioxidants, chelating agents, colorants, etc. These can be used alone or in combination of two or more.

[0060] (Base or carrier) Examples of bases or carriers include inorganic powders such as talc, mica, sericite, kaolin, bentonite, barium sulfate, and anhydrous silicic acid; organic powders such as dextrin, crystalline cellulose, and starch; lower alcohols such as ethanol and isopropanol; glycol ethers such as ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monopropyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monopropyl ether, diethylene glycol monobutyl ether, propylene glycol monoethyl ether, propylene glycol monopropyl ether, dipropylene glycol monoethyl ether, and dipropylene glycol monopropyl ether; polyhydric alcohols such as polyethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, concentrated glycerin, isoprene glycol, diglycerin, dipropylene glycol, and sorbitol; liquid paraffin, squalane, gelling hydrocarbons (such as Plastibase), and ethanol. Hydrocarbons such as zokerite and light liquid paraffin; silicone oils such as methylpolysiloxane, crosslinked methylpolysiloxane, highly polymerized methylpolysiloxane, cyclic silicone, alkyl-modified silicone, crosslinked alkyl-modified silicone, amino-modified silicone, polyether-modified silicone, polyglycerin-modified silicone, crosslinked polyether-modified silicone, crosslinked alkylpolyether-modified silicone, silicone / alkyl chain co-modified polyether-modified silicone, silicone / alkyl chain co-modified polyglycerin-modified silicone, polyether-modified branched silicone, polyglycerin-modified branched silicone, acrylic silicone, phenyl-modified silicone, and silicone resin; oils and fats such as coconut oil, olive oil, rice bran oil, shea butter, rosehip oil, and almond oil; waxes such as jojoba oil, bee wax, candelilla wax, and lanolin; higher alcohols such as cetyl alcohol, cetostearyl alcohol, stearyl alcohol, behenyl alcohol, octyldodecanol, isostearyl alcohol, phytosterol, and cholesterol; dioxane;Examples of the esters include isopropyl myristate, octyldodecyl myristate, isopropyl palmitate, cetyl palmitate, isononyl isononanoate, and pentaerythritol tetra-2-ethylhexanoate. These may be used alone or in combination of two or more.

[0061] The total content of the base or carrier can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is, for example, 45 to 90% by mass, 50 to 85% by mass, 55 to 80% by mass, 60 to 75% by mass, etc., relative to the total amount of the topical skin composition (100% by mass).

[0062] (surfactant) Examples of surfactants include anionic surfactants, nonionic surfactants, amphoteric surfactants, cationic surfactants, naturally occurring surfactants, etc. These can be used alone or in combination of two or more.

[0063] The total content of the surfactant can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is, for example, 0.1 to 5 mass%, 0.2 to 4 mass%, 0.3 to 3 mass%, 0.4 to 2 mass%, etc., relative to the total amount (100 mass%) of the topical skin composition.

[0064] Examples of anionic surfactants include higher fatty acids such as palmitic acid, lauric acid, myristic acid, and stearic acid; higher fatty acid salts such as sodium palmitate, potassium laurate, sodium myristate, and potassium stearate; amino acid surfactants such as cocoyl glutamate, cocoyl methyl taurate sodium, cocoyl methyl taurate taurate, cocoyl glycine salt, stearoyl glutamate, myristoyl glutamate, and lauroyl glutamate; alkyl sulfates such as sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium laureth sulfate, sodium myristyl sulfate, sodium stearyl sulfate, sodium oleyl sulfate, and sodium cetyl sulfate; polyoxyethylene alkyl ether sulfates such as polyoxyethylene lauryl ether sodium sulfate and polyoxyethylene myristyl ether sodium sulfate; alkyl sulfosuccinates such as dioctyl sodium sulfosuccinate and lauryl disodium sulfosuccinate; and polyoxyethylene lauryl disodium sulfosuccinate. monoalkyl phosphate salts such as sodium lauryl phosphate, sodium cetyl phosphate, and diethanolamine cetyl phosphate; polyoxyethylene alkyl ether phosphate salts such as polyoxyethylene lauryl ether sodium phosphate, polyoxyethylene cetyl ether sodium phosphate, polyoxyethylene oleyl ether sodium phosphate, polyoxyethylene alkylphenyl ether sodium phosphate, and polyoxyethylene alkylphenyl ether triethanolamine phosphate; alkyl ether glycol acetates such as sodium lauryl glycol acetate (sodium dodecane-1,2-diol acetate), potassium lauryl glycol acetate, sodium myristyl glycol acetate, potassium myristyl glycol acetate, sodium palmityl glycol acetate, potassium palmityl glycol acetate, sodium stearyl glycol acetate, potassium stearyl glycol acetate, sodium behenyl glycol acetate, and potassium behenyl glycol acetate; and the like.

[0065] The total content of the anionic surfactant can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, for example, 0.1 to 45% by mass, 0.5 to 40% by mass, 1 to 35% by mass, 2 to 30% by mass, 2.5 to 25% by mass, etc., relative to the total amount (100% by mass) of the topical skin composition.

[0066] Examples of nonionic surfactants include sorbitan fatty acid esters such as sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, diglycerol sorbitan penta-2-ethylhexylate, and diglycerol sorbitan tetra-2-ethylhexylate; glycerin fatty acid esters such as lipophilic glyceryl monostearate, self-emulsifying glyceryl monostearate, and glycerin monostearate malate; polyglyceryl monostearate, monoisostearate, and glycerin monoisostearate; Polyglycerin fatty acid esters such as polyglyceryl esters of lactic acid, polyglyceryl diisostearate, polyglyceryl monolaurate, polyglyceryl monooleate, and polyglyceryl monomyristate; propylene glycol fatty acid esters such as propylene glycol monostearate; ethylene glycol distearate, ethylene glycol monostearate, triethylene glycol distearate, polyethylene glycol monostearate (2 E.O.), polyethylene glycol monostearate (4 E.O.), polyethylene glycol monostearate (9 E.O.), polyethylene glycol monostearate (10 E.O.), polyethylene glycol monostearate (23 E.O.), polyethylene glycol monostearate (25 E.O.), polyethylene glycol monostearate (32 E.O.), polyethylene glycol monostearate (40 E.O.), polyethylene glycol monostearate (45 E.O.), polyethylene glycol monostearate (55 E.O.), and polyglycerin monostearate. Ethylene glycol fatty acid esters such as ethylene glycol (75 E.O.), polyethylene glycol monostearate (140 E.O.), polyethylene glycol distearate (140 E.O.), and polyethylene glycol distearate (250 E.O.); hydrogenated castor oil derivatives such as polyoxyethylene hydrogenated castor oil 40 (HCO-40), polyoxyethylene hydrogenated castor oil 50 (HCO-50), polyoxyethylene hydrogenated castor oil 60 (HCO-60), and polyoxyethylene hydrogenated castor oil 80 (HCO-80);Polyoxyethylene (20) sorbitan fatty acid esters such as polyoxyethylene (20) sorbitan monolaurate (polysorbate 20), polyoxyethylene (20) sorbitan monostearate (polysorbate 60), polyoxyethylene (20) sorbitan monooleate (polysorbate 80), and polyoxyethylene (20) sorbitan isostearate; caprylic acid N-methylethanolamide, capric acid N-methylethanolamide, lauric acid N-methylethanolamide, lauric acid N-ethylethanolamide, and lauric acid N -methylpropanolamide, lauric acid N-ethylpropanolamide, lauric acid N-methylisopropanolamide, lauric acid N-ethylisopropanolamide, myristic acid N-methylethanolamide, palmitic acid N-methylethanolamide, coconut oil fatty acid N-methylethanolamide, coconut oil fatty acid N-ethylethanolamide, coconut oil fatty acid N-methylpropanolamide, coconut oil fatty acid N-ethylpropanolamide, coconut oil fatty acid N-methylisopropanolamide, coconut oil fatty acid N-ethylisopropanolamide, Fatty acid alkanolamides such as coconut oil fatty acid diethanolamide, palm kernel oil fatty acid N-methylethanolamide, palm kernel oil fatty acid N-ethylethanolamide, stearic acid N-methylethanolamide, stearic acid N-ethylethanolamide, oleic acid N-methylethanolamide, and oleic acid N-ethylethanolamide; polyoxyethylene monococoate glyceryl; glycerin alkyl ether; hexyl glucoside, capryl glucoside, nonyl glucoside, decyl glucoside, lauryl glucoside, myristyl glucoside Examples of suitable surfactants include alkyl glucosides such as cetostearyl glucoside, hexadecyl glucoside, octadecyl glucoside, and coconut oil alkyl glucoside; polyoxyalkylene alkyl ethers such as polyoxyethylene lauryl ether, polyoxyethylene cetyl ether, polyoxyethylene stearyl ether, polyoxyethylene oleyl ether, and polyoxyethylene behenyl ether; and silicone surfactants such as lauryl PEG-9 polydimethylsiloxyethyl dimethicone and PEG-9 polydimethylsiloxyethyl dimethicone.

[0067] The total content of the nonionic surfactant can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, for example, 0.1 to 20 mass%, 0.5 to 15 mass%, 1 to 12 mass%, 2 to 10 mass%, 2.5 to 8 mass%, etc., relative to the total amount (100 mass%) of the topical skin composition.

[0068] Examples of amphoteric surfactants include coconut oil fatty acid amidopropyl betaine, lauryl hydroxysulfobetaine, lauric acid amidopropyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, N-lauroyl-N'-carboxymethyl-N'-hydroxyethyl ethylenediamine sodium, lauramidopropyl hydroxybetaine, lauric acid amidopropyl dimethylamine oxide, and hydroxyalkyl (C12-14) hydroxyethyl sarcosine.

[0069] The total content of the amphoteric surfactant can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, for example, 0.1 to 20% by mass, 0.5 to 15% by mass, 1 to 12% by mass, 2 to 10% by mass, 2.5 to 8% by mass, etc., relative to the total amount (100% by mass) of the topical skin composition.

[0070] Examples of cationic surfactants include stearyltrimethylammonium chloride, alkyltrimethylammonium chloride, cetyltrimethylammonium chloride, behenyltrimethylammonium chloride, behenyldimethylhydroxyethylammonium chloride, stearyldimethylbenzylammonium chloride, distearyldimethylammonium chloride, dicetylmethylammonium chloride, cetyltriethylammonium methyl sulfate, stearyltrimethylammonium bromide, and cetyltrimethylammonium bromide.

[0071] The total content of the cationic surfactant can be appropriately set depending on the type and amount of other ingredients, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is, for example, 0.1 to 5 mass%, 0.2 to 4 mass%, 0.3 to 3 mass%, 0.4 to 2 mass%, etc., relative to the total amount (100 mass%) of the topical skin composition.

[0072] Examples of naturally occurring surfactants include lecithin, hydrogenated lecithin, saponin, sodium surfactin, bile acid, and the like.

[0073] The total content of naturally occurring surfactants can be appropriately set depending on the types and amounts of other ingredients, dosage form, etc., and is not limited, but from the viewpoint of significantly achieving the effects of the present invention, it is, for example, 0.1 to 5 mass%, 0.2 to 4 mass%, 0.3 to 3 mass%, 0.4 to 2 mass%, etc., relative to the total amount (100 mass%) of the topical skin composition.

[0074] (thickener) Examples of thickeners include gums (gellan gum, xanthan gum, sclerotium gum, locust bean gum, biosaccharide gum, tamarind gum, quince seed, gum arabic, tara gum, guar gum, galactan, gum arabic, tragacanth gum, etc.), carrageenan, curdlan, succinoglucan, heparinoids, alginates (alginic acid, sodium alginate, propylene glycol alginate, etc.), agar (including agarose), gelatin, pectin, pullulan, mannan, vinyl thickeners (polyvinyl alcohol, polyvinylpyrrolidone, polyvinyl methyl ether, carboxyvinyl polymer, etc.), cellulose thickeners (methylcellulose, ethylcellulose, etc.), and the like. Examples of suitable cellulose acetate copolymers include cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, carboxymethyl cellulose, carboxyethyl cellulose, hydrophobized hydroxypropyl methylcellulose, etc.), dextran, dextrin, acrylates / alkyl methacrylate copolymers, sodium polyacrylate, bentonite, dextrin fatty acid esters, dimethyl distearyl ammonium hectorite, polyethylene glycol, macrogol, (hydroxyethyl acrylate / sodium acryloyldimethyltaurate) copolymer, (ammonium acryloyldimethyltaurate / vinylpyrrolidone) copolymer, etc. The total content of the thickener can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but may be, for example, 0.001 to 5% by mass, 0.01 to 3% by mass, 0.05 to 1% by mass, etc., relative to the total amount (100% by mass) of the topical skin composition.

[0075] (preservatives) Examples of preservatives include isopropylmethylphenol, chlorobutanol, benzyl alcohol, phenethyl alcohol, benzoic acid, sodium benzoate, dehydroacetic acid, sodium dehydroacetate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, butyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, benzyl parahydroxybenzoate, methyl parahydroxybenzoate (methylparaben), phenoxyethanol, ethylhexylglycerin, glyceryl caprate, pentanediol, hexanediol, and chlorphenesin. The total content of antiseptics and preservatives can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0076] (pH adjuster) The pH adjuster is not particularly limited, but examples thereof include inorganic bases such as potassium hydroxide, sodium hydroxide, and sodium carbonate; organic bases such as triethanolamine, diisopropanolamine, and triisopropanolamine; inorganic acids such as hydrochloric acid and sulfuric acid; and organic acids such as lactic acid, sodium lactate, citric acid, sodium citrate, succinic acid, and sodium succinate. These may be used alone or in combination of two or more. Among these, potassium hydroxide and sodium hydroxide are preferred. The total content of the pH adjuster can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0077] (antioxidant) Examples of antioxidants include dibutylhydroxytoluene (BHT), butylhydroxyanisole, sorbic acid, sodium pyrosulfite, erythorbic acid, and L-cysteine ​​hydrochloride. The total content of the antioxidant can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0078] (chelating agent) Examples of chelating agents include EDTA disodium salt, EDTA calcium disodium salt, and gluconolactone. The total content of the chelating agent can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0079] (coloring agent) Examples of colorants include inorganic pigments and natural dyes. The total content of the colorant can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 1 mass%, preferably 0.005 to 0.1 mass%, and more preferably 0.01 to 0.05 mass%, relative to the total amount of the topical skin composition (100 mass%).

[0080] The present topical skin composition may contain other active ingredients as long as the effects of the present invention are not impaired. Specific examples of active ingredients include, but are not limited to, moisturizing ingredients, scrubbing agents, blood circulation promoters, astringent ingredients, UV-absorbing ingredients, antibacterial ingredients, anti-inflammatory agents, vitamins, peptides or derivatives thereof, amino acids or derivatives thereof, and cell-activating ingredients. These may be used alone or in combination of two or more.

[0081] (moisturizing ingredient) Examples of moisturizing ingredients include polyhydric alcohols such as 1,3-butylene glycol, 1,3-propanediol, propylene glycol, pentanediol, glycerin, diglycerin, sorbitol, and polyethylene glycol; chondroitin sulfate or a salt thereof (sodium chondroitin sulfate, potassium chondroitin sulfate, sodium dermatan sulfate, potassium dermatan sulfate, etc.); PPG-17 buteth-17, PPG-25 sorbitol, polyoxyalkylene alkyl glucoside, PEG / PPG / polybutylene glycol-8 / 5 / 3 glycerin, and polyoxyalkylene diglyceryl. Alkylene oxides; glycosyltrehalose, trehalose; ceramide, glucosylceramide, cholesterol, phytosterol, cholesterol derivatives, phytosterol derivatives; 2-methacryloyloxyethyl phosphorylcholine-butyl methacrylate copolymer liquid, polymethacryloyloxyethyl phosphorylcholine and other 2-methacryloyloxyphosphorylcholine-containing polymers; NMF-derived components such as lactic acid, sodium lactate, sodium pyrrolidone carboxylate, urea, etc.; collagen, elastin, keratin, chitin, chitosan, etc. and their hydrolysates; hydroxyethyl urea, etc. The total content of moisturizing ingredients can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 60% by mass, 0.01 to 50% by mass, preferably 0.03 to 40% by mass, and more preferably 0.05 to 30% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0082] (scrubbing agent) Examples of scrubbing agents include apricot kernel powder, almond shell powder, sodium chloride granules, olive kernel powder, candelilla wax, walnut shell powder, cherry kernel powder, coral powder, charcoal powder (Paulownia charcoal, Binchotan charcoal, bamboo charcoal, cypress charcoal, coconut shell charcoal, activated carbons thereof, and medicinal carbons thereof), hazelnut shell powder, and polyethylene powder. The total content of the scrubbing agent can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.01 to 15% by mass, preferably 0.05 to 10% by mass, and more preferably 0.1 to 5% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0083] (blood circulation promoter) Examples of blood circulation promoters include acetylcholine, ichthammol, caffeine, capsaicin, cantharides tincture, γ-oryzanol, rhododendron tincture, zingerone, cepharanthine, Swertia japonica extract, tannic acid, capsicum tincture, tolazoline, tocopherol nicotinate, and benzyl nicotinate. The total content of the blood circulation promoter can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 5% by mass, preferably 0.005 to 3% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0084] (astringent ingredient) Examples of astringent ingredients include zinc sulfate, aluminum hydroxide, aluminum chloride, zinc sulfophenol, and tannic acid. The total content of the astringent component can be set appropriately depending on the type and amount of other components, dosage form, etc., and is not limited to, but is, for example, 0.001 to 20% by mass, preferably 0.01 to 15% by mass, and more preferably 0.1 to 10% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0085] (UV absorbing component) Examples of ultraviolet absorbing components include octyl triazone, diethylaminohydroxybenzoylhexyl benzoate, dimethoxybenzylidene dioxoimidazolidine octyl propionate, 2-ethylhexyl paramethoxycinnamate, t-butylmethoxydibenzoylmethane, phenylbenzimidazole sulfonic acid, octyl methoxycinnamate, and ethylhexyl methoxycinnamate. The total content of the ultraviolet absorbing component can be set appropriately depending on the type and amount of other components, dosage form, etc., and is not limited to, but is, for example, 1 to 30% by mass, preferably 3 to 25% by mass, and more preferably 5 to 20% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0086] (Antibacterial ingredient) Examples of antibacterial ingredients include isopropylmethylphenol, chlorhexidine, benzalkonium chloride, acrinol, benzethonium chloride, cresol, gluconic acid and its derivatives, povidone-iodine, potassium iodide, iodine, triclocarban, triclosan, photosensitizer No. 101, photosensitizer No. 201, paraben, phenoxyethanol, alkyldiaminoglycine hydrochloride, piroctoolamine, and miconazole. The total content of the antibacterial component can be set appropriately depending on the type and amount of other components, dosage form, etc., and is not limited to, but is, for example, 0.001 to 2.5% by mass, preferably 0.005 to 2% by mass, and more preferably 0.01 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0087] (anti-inflammatory agent) Examples of anti-inflammatory agents include non-steroidal anti-inflammatory agents and steroidal anti-inflammatory agents. Specific examples include glycyrrhizinic acid, glycyrrhetinic acid, stearyl glycyrrhetinate, allantoin, epsilon aminocaproic acid, salicylic acid, methyl salicylate, glycol salicylate, indomethacin, felbinac, ibuprofen, ibuprofen piconol, ketoprofen, bufexamac, butyl flufenamate, bendazac, piroxicam, suprofen, azulene, guaiazulene, dexamethasone valerate acetate, dexamethasone, prednisolone valerate acetate (prednisolone valerate acetate), prednisolone acetate, prednisolone, hydrocortisone acetate, hydrocortisone, ufenamate, bufexamac, and salts thereof. The total content of the anti-inflammatory agent can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 5% by mass, preferably 0.005 to 4.5% by mass, and more preferably 0.01 to 3% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0088] (Vitamins) Vitamins include vitamin E such as dl-α-tocopherol, dl-α-tocopherol succinate, and dl-α-tocopherol calcium succinate; vitamin B2 such as riboflavin, flavin mononucleotide, flavin adenine dinucleotide, riboflavin butyrate, riboflavin tetrabutyrate, riboflavin 5'-phosphate sodium, and riboflavin tetranicotinate; dl-α-tocopherol nicotinate, benzyl nicotinate, methyl nicotinate, β-butoxyethyl nicotinate, and nicotine. Nicotinic acids such as 1-(4-methylphenyl)ethyl acetate; Vitamin C compounds such as ascorbigen-A, ascorbyl stearate, ascorbyl palmitate, and L-ascorbyl dipalmitate; Vitamin D compounds such as methylhesperidin, ergocalciferol, and cholecalciferol; Vitamin K compounds such as phylloquinone and farnoquinone; γ-oryzanol, dibenzoylthiamine, and dibenzoylthiamine hydrochloride; thiamine hydrochloride, thiamine cetyl hydrochloride, thiamine thiocyanate, thiamine lauryl hydrochloride, and thiamine mononitrate. Vitamin B1 compounds such as thiamine monophosphate, thiamine lysine salt, thiamine triphosphate, thiamine monophosphate phosphate, thiamine monophosphate ester, thiamine diphosphate ester, thiamine diphosphate ester hydrochloride, thiamine triphosphate, thiamine triphosphate monophosphate; Vitamin B6 compounds such as pyridoxine hydrochloride, pyridoxine acetate, pyridoxal hydrochloride, pyridoxal 5'-phosphate, pyridoxamine hydrochloride; Vitamin B12 compounds such as cyanocobalamin, hydroxocobalamin, deoxyadenosylcobalamin folic acids such as folic acid and pteroylglutamic acid; nicotinic acids such as nicotinic acid and nicotinamide; pantothenic acids such as pantothenic acid, calcium pantothenate, pantothenyl alcohol (panthenol), D-pantethine, D-pantethine, coenzyme A, and pantothenyl ethyl ether; biotins such as biotin and bioticin; ascorbic acid derivatives such as vitamin C, ascorbic acid, sodium ascorbate, dehydroascorbic acid, sodium ascorbyl phosphate, and magnesium ascorbyl phosphate;Vitamin-like factors such as carnitine, ferulic acid, α-lipoic acid, and orotic acid are included. The total content of vitamins can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 25% by mass, preferably 0.01 to 20% by mass, and more preferably 0.05 to 15% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0089] (Peptide or its derivative) Examples of peptides or derivatives thereof include keratin hydrolyzed peptides, hydrolyzed keratin, collagen, fish-derived collagen, atelocollagen, gelatin, elastin, elastin hydrolyzed peptides, collagen hydrolyzed peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin hydrolyzed peptides, conchiolin hydrolyzed peptides, hydrolyzed conchiolin, silk proteolytic peptides, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soybean proteolytic peptides, hydrolyzed soybean protein, wheat protein, wheat proteolytic peptides, hydrolyzed wheat protein, casein hydrolyzed peptides, and acylated peptides (palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide, etc.). The total content of the peptide or its derivative can be set appropriately depending on the type and amount of other ingredients, dosage form, etc., and is not limited to, for example, 0.001 to 5% by mass, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0090] (amino acid or its derivative) Examples of amino acids and derivatives thereof include betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, and creatine. The total content of amino acids or derivatives thereof can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, for example, 0.001 to 5% by mass, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0091] (Cell activation component) Examples of cell activating components include α-hydroxy acids such as glycolic acid and lactic acid, tannin, flavonoid, saponin, photosensitizer No. 301, and the like. The total content of the cell-activating ingredients can be set appropriately depending on the types and amounts of other ingredients, dosage form, etc., and is not limited to, but is, for example, 0.001 to 5% by mass, preferably 0.01 to 3% by mass, and more preferably 0.05 to 1% by mass, relative to the total amount of the topical skin composition (100% by mass).

[0092] [pH of topical skin composition] The pH of the present composition for external use on skin can be appropriately set depending on the dosage form, etc., and is not limited to, for example, preferably pH 4.0 to 11.0, more preferably pH 5.0 to 10.0, even more preferably pH 5.0 to 9.5, and particularly preferably pH 5.5 to 9.5. The pH is measured at room temperature (25°C) using a pH meter (for example, pH METER F-52 (manufactured by HORIBA)). When the composition for external use on skin is in a powder form (for example, a facial cleansing powder), the pH is measured as the pH of an aqueous solution with a concentration of 5% by mass.

[0093] [Viscosity of topical skin composition] The viscosity (25°C) of the present topical skin composition can be appropriately set depending on the dosage form, etc. For example, it is 100,000 to 5,000,000 mPa·s, preferably 150,000 to 3,000,000 mPa·s, more preferably 250,000 to 2,000,000 mPa·s, and most preferably 300,000 to 2,000,000 mPa·s. In another embodiment, the viscosity is preferably 1 to 300 mPa·s, more preferably 5 to 200 mPa·s, and even more preferably 10 to 100 mPa·s.

[0094] The viscosity (25°C) is measured using a single-cylindrical rotational viscometer (Brookfield viscometer) in accordance with the viscosity measurement method described in the General Test Methods of the Japanese Pharmacopoeia, 18th Edition. Specifically, it refers to a value measured using a TV-10M (manufactured by Toki Sangyo Co., Ltd.), with the rotor, rotation speed, and other conditions selected in accordance with the instrument's instruction manual, and the viscosity measured at 25°C. More specifically, the viscosity is measured at 25°C using an M4 rotor at a rotation speed of 0.3 rpm for a measurement time of 180 seconds. In embodiments where the viscosity of the topical skin composition is low, the viscosity may be measured using, for example, a TV-100EL (manufactured by Toki Sangyo Co., Ltd.), with the rotor, rotation speed, and other conditions selected in accordance with the device's instruction manual, and the viscosity measured at 25°C. More specifically, the measurement may be performed under the following conditions: a sample volume of 1.1 mL, a rotor diameter of 4.7 cm, a rotation speed of 5 rpm, and a range of "M" for viscosities of 100 mPa·s or less, "2.5M" for viscosities between 100 and 200 mPa·s, and "5M" for viscosities of 200 mPa·s or more.

[0095] The single cylinder rotational viscometer is described below. A single cylinder rotational viscometer is a viscometer that measures the torque when a cylinder is rotated at a constant angular velocity in a liquid. By experimentally determining the instrument constant KB in advance using a standard liquid for viscometer calibration, the viscosity η of the liquid can be calculated using the following equation. η=KB×T / ω η: Viscosity of the liquid (mPa·s) KB: Equipment constant (rad / cm 3 ) ω: Angular velocity (rad / s) T: Torque acting on the cylindrical surface (10 -7 N·m)

[0096] [Dosage form and formulation of topical skin composition] The topical skin composition is prepared in various forms by blending the above-mentioned components and, if necessary, further components, according to a conventional method. The topical skin composition is provided in any form that can be widely used as a cosmetic, quasi-drug, pharmaceutical, etc. Furthermore, the present topical skin composition is not particularly limited, and may be provided as, for example, a powder or granules, a lotion, a gel, a mousse, a liquid, a suspension, an emulsion, a cream, an ointment, a gel, a liniment, or a wipe-off sheet such as a facial sheet or a body sheet in which the composition is impregnated into a sheet such as a nonwoven fabric.

[0097] [Method of manufacturing a composition for external use on skin] The present composition for external use on skin can be produced by a known method, for example, by mixing the components in accordance with or in accordance with the 18th Edition of the Japanese Pharmacopoeia.

[0098] [Container for topical skin composition] The topical skin composition can be filled into a container appropriately selected depending on the intended purpose and application. Examples of such containers include bottles, tubes, jars, dispensers, pouches, and spouted pouches. Materials for the container include polyethylene terephthalate, polypropylene, polyethylene (HDPE, LDPE, LLDPE, etc.), ABS resin, ethylene vinyl alcohol resin, polystyrene, glass, metal (aluminum, etc.), and mixtures thereof. Containers made from these materials can be coated with various coatings to improve strength, flexibility, weather resistance, or component stability. Containers can also be formed by laminating films made from the above materials.

[0099] In one embodiment of the present topical skin composition, the composition is preferably filled into a foam-dispensing container in order to provide a cleansing composition that is easy to use. Conventional containers can be used as the foam-dispensing container. Specific examples include squeeze foamers that dispense foam by pressing the body of a soft container with fingers, and pump foamers that dispense foam by pressing the top of a cap equipped with a pump mechanism with fingers. A pump foamer is, for example, a non-gas foam-dispensing container that mixes a certain amount of liquid skin cleansing composition with a certain amount of air and dispenses it in a foam state.

[0100] In one embodiment of the present topical skin composition, the composition is preferably filled into a tube-shaped container in order to provide a cleansing composition that is easy to use. Known tube-shaped containers can be used as the tube-shaped container (see, for example, but not limited to, JP 2000-272643 A, JP 2004-148628 A, JP 2005-022682 A, etc.). A specific example of a tube-shaped container is a container having a main body containing the cleansing composition and a cap. The main body has a cylindrical body formed by rolling a single-layer or laminated sheet (total thickness of the sheet, for example, about 0.1 to 1 mm) made of a synthetic resin such as polyethylene, overlapping both ends, and heat-sealing it; a bottom seal formed by heat-sealing one end of the body into a flat shape; and a head provided at the other end of the body. The head has a shoulder and an outlet provided approximately in the center of the shoulder, and the outlet is covered with a cap that can be opened and closed. A user can squeeze the body with his / her hand and apply pressure to push out the contents of the container (cleaning composition) for use.

[0101] [Uses of topical skin compositions] The uses of the present topical skin composition are not particularly limited, and examples thereof include skin cleansers such as body soap (also called body shampoo), hand soap, facial cleanser, makeup remover, hair shampoo, dry shampoo, and rinse-in shampoo; basic cosmetics such as serum, lotion, sunscreen cream, emulsion, cream, lotion, oil, and pack; and makeup cosmetics such as foundation, lipstick, lip balm, mascara, eye shadow, eyeliner, eyebrow pencil, and nail polish.

[0102] The present composition for external use on the skin is preferably used as a skin cleansing composition to be applied to the skin (including the face, body, and scalp). In particular, a cleansing composition for the face and / or body is preferred. Furthermore, in one embodiment of the present invention, from the viewpoint of excellent cleansing properties, particularly excellent sebum cleansing ability, the composition is suitable as a skin cleansing composition to be used on any area where sebum is actively secreted, such as the face, back, abdomen, chest, or inside of joints, and is suitable as a cleansing composition or sebum cleanser. It is also suitable as a cleansing composition for oily skin, which tends to produce excessive sebum. It is also suitable as a cleansing composition for pores, where waste products such as keratin tend to accumulate.

[0103] [Examples of preferred embodiments of the composition for external use on skin] As mentioned above, the present composition for external use on skin can effectively suppress the decrease in the activity of proteases and / or lipases, and therefore has an excellent effect of decomposing keratin and sebum. From this perspective, an example of a preferred embodiment of the present invention is a skin cleansing composition containing (A) a protease and / or a lipase, and (B) one or more members selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts.

[0104] (Powdered skin cleansing composition) Among the skin cleansing compositions, one preferred embodiment is a powdered skin cleansing composition (e.g., a facial cleansing powder). The powdered skin cleansing composition can be appropriately produced using the component (A), the component (B), and the "other components" described above, etc., using a known method. When component (B) is used in a powdered skin cleansing composition (for example, a facial cleansing powder) together with a protease or a lipolytic enzyme, the decrease in enzyme activity over time can be suppressed. This reduces or eliminates concerns about the decrease in enzyme activity over time due to external factors (such as humidity in the manufacturing environment of cosmetics, distribution environment in the market, and usage environment of consumers), and also has an excellent effect in eliminating blackening of pores, clogged keratin plugs, roughness, etc.

[0105] The term "powdered" refers to a solid in powder or granular form composed of particles or fine particles, specifically, for example, particles having a particle size of 500 μm or less, each of which is fluid at room temperature and pressure. The particle size of the powdered topical skin composition is not particularly limited, but is preferably, for example, 5 to 500 μm, and more preferably 5 to 300 μm. From the viewpoints of, for example, solubility in water and usability, it is preferable that the powdered topical skin composition pass through a sieve (mesh) with openings of approximately 500 μm.

[0106] Powdered skin cleansing compositions are prepared appropriately using known methods using the above-mentioned component (A), component (B), and the aforementioned "other components" as raw materials, and examples thereof include, but are not limited to, skin cleansing compositions containing (A) protease and / or lipolytic enzyme, (B) inulin, inositol, seaweed extract, Geranium globulus extract, and one or more components selected from the group consisting of hyaluronic acid and its salts, as well as inorganic powders such as talc, and surfactants such as amino acid-based surfactants.

[0107] Examples of the inorganic powder include talc, sericite, mica, kaolin, montmorillonite, clay, bentonite, vermiculite, silicic acid, silicic anhydride (silica), magnesium silicate, calcium silicate, aluminum silicate, barium silicate, strontium silicate, diatomaceous earth, mica titanium, bismuth oxychloride, boron nitride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, low-order titanium oxide, metal tungstate, hydroxyapatite, zeolite, ceramic powder, aluminum chlorohydrate, aluminum chloride, aluminum sulfate, basic aluminum bromide, basic aluminum iodide, aluminum chlorohydrate zirconium, zinc sulfate, basic aluminum zinc lactate, aluminum oxide, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, red iron oxide, black iron oxide, yellow iron oxide, ultramarine, iron blue, chromium oxide, chromium hydroxide, calamine, and carbon black. Among these, from the viewpoint of feel during use, lathering, and the like, one or more selected from the group consisting of talc, sericite, silicic acid anhydride (silica), mica, and kaolin are preferred.

[0108] The total content of the inorganic powder relative to the total amount (100% by mass) of the powdered skin cleansing composition is not limited, but is, for example, 1 to 80% by mass, preferably 5 to 80% by mass, more preferably 10 to 75% by mass, and even more preferably 15 to 70% by mass.

[0109] As the surfactant, amino acid surfactants are preferred from the viewpoint of mild action on the skin, etc. Examples of amino acid surfactants include N-acyl amino acid surfactants. The amino acids constituting the N-acyl amino acid surfactants may be neutral, acidic, or basic, and may be in the L-, D-, or DL-form. Specific examples include glycine, alanine, sarcosine, valine, leucine, isoleucine, aspartic acid, glutamic acid, etc. Examples of acyl groups constituting N-acyl amino acid surfactants include acyl groups derived from saturated or unsaturated, straight-chain or branched-chain fatty acids having 6 to 22 carbon atoms. Acyl groups derived from saturated fatty acids having 6 to 18 carbon atoms are preferred. The acyl groups may be a single acyl group, an acyl group derived from beef tallow fatty acid, or a mixture thereof. Specific examples of fatty acids include lauric acid, palmitic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, behenic acid, coconut oil fatty acid, palm oil fatty acid, and beef tallow fatty acid.

[0110] Examples of the base component constituting the N-acyl amino acid surfactant include alkali metals such as sodium and potassium, alkaline earth metals such as magnesium and calcium, organic amines such as monoethanolamine, diethanolamine, triethanolamine, and 2-amino-2-methyl-1-propanol, basic amino acids such as lysine, ornithine, and arginine, and ammonia.

[0111] Specific examples of N-acyl amino acid surfactants include N-acyl glutamic acid surfactants, N-acyl glycine surfactants, N-acylalanine surfactants, N-acylsarcosinic acid surfactants, and N-acylaspartic acid surfactants.

[0112] The total content of surfactants relative to the total amount (100% by mass) of the powdery skin cleansing composition is not limited, but is, for example, 5 to 70% by mass, preferably 5 to 60% by mass, more preferably 10 to 50% by mass, and even more preferably 15 to 45% by mass.

[0113] The total content of amino acid-derived surfactants relative to the total amount (100% by mass) of the powdered skin cleansing composition is not limited, but is, for example, 5 to 70% by mass, preferably 5 to 60% by mass, more preferably 10 to 50% by mass, and even more preferably 15 to 45% by mass.

[0114] Furthermore, powdered skin cleansing compositions may contain organic powders from the viewpoints of usability, moisturizing properties, etc. Examples of organic powders include monosaccharides such as glucose, fructose, galactose, and mannose; disaccharides such as lactose, trehalose, and maltose; starches such as cornstarch and potato starch; polysaccharides such as dextrin and (crystalline) cellulose; and sugar alcohols such as sorbitol, mannitol, maltitol, xylitol, and erythritol.

[0115] The total content of the organic powder relative to the total amount (100% by mass) of the powdered skin cleansing composition is not limited, but is, for example, 1 to 75% by mass, preferably 5 to 70% by mass, more preferably 10 to 65% by mass, and even more preferably 15 to 60% by mass.

[0116] The powdered skin cleansing composition may also contain a water-soluble polymer. Examples of water-soluble polymers include polymers, copolymers, or crosspolymers containing (meth)acrylic acid monomers and / or alkyl (meth)acrylate monomers as constituent components, such as acrylic polymers commonly used in topical compositions and cosmetics. Specific examples include, but are not limited to, acrylic acid-alkyl methacrylate copolymers, hydroxyethyl acrylate-acryloyldimethyl taurate copolymers, polyacrylic acid or salts thereof, and carboxyvinyl polymers. Examples of water-soluble polymers include cationized cellulose such as O-[2-hydroxy-3-(trimethylammonio)propyl]hydroxyethyl cellulose chloride (polyquaternium-10) and hydroxyethyl cellulose dimethyldiallylammonium chloride (polyquaternium-4). Examples of water-soluble polymers include dimethyldiallylammonium chloride-acrylamide copolymer (Polyquaternium-7), acrylamide-acrylic acid-dimethyldiallylammonium chloride copolymer (Polyquaternium-39), and dimethyldiallylammonium chloride-acrylic acid copolymer (Polyquaternium-22).

[0117] The total content of the water-soluble polymer relative to the total amount (100% by mass) of the powdery skin cleansing composition is not limited, but is, for example, 0.001 to 10% by mass, preferably 0.005 to 8% by mass, more preferably 0.01 to 5% by mass, and even more preferably 0.1 to 3% by mass.

[0118] In order to prevent a decrease in enzyme activity, it is preferable that the powdery composition for external use on skin is substantially free of (C) water. The total content of (C) water in the powdery composition for external use on skin is not particularly limited, but is preferably 5% by mass or less, more preferably 1% by mass or less, even more preferably 0.5% by mass or less, and particularly preferably 0.3% by mass or less, relative to the total amount (100% by mass) of the powdery composition for external use on skin.

[0119] The pH of the powdery skin cleansing composition is not limited from the viewpoint of preventing a decrease in enzyme activity, but is preferably, for example, pH 4.0 to 11.0, more preferably pH 5.0 to 10.0, even more preferably pH 5.0 to 9.5, and particularly preferably pH 5.5 to 9.5. As described above, the pH is measured as the pH (25°C) of a 5% by mass aqueous solution.

[0120] A method for using the powdered skin cleansing composition includes, for example, placing preferably 0.2 to 2 g, more preferably 0.2 to 0.8 g, of the powdered skin cleansing composition in the palm of your hand, adding an appropriate amount of water (preferably lukewarm water), for example 1 to 5 mL, preferably 1 to 3 mL, to dissolve the composition, applying the aqueous solution of the cleansing composition evenly to the area to be cleaned (for example, the entire facial skin), removing dirt and the like from the skin, and then rinsing thoroughly with water or lukewarm water.

[0121] In addition, as an example of a preferred embodiment of a powdered skin cleansing composition, it is preferably filled into a container divided into single-use amounts. For example, but not limited to, it is preferably filled into a portion-type container that includes a container body having a storage section capable of storing the powdered topical skin composition, a flange section surrounding the upper opening of the storage section and extending outward, and a lid sheet laminated on the flange section to cover and seal the upper opening.

[0122] The powdered skin cleansing composition can be produced by a conventional method, such as by granulating a mixture of the above-mentioned components into a powder using agitation granulation, tumbling granulation, extrusion granulation, fluidized bed granulation, or the like.

[0123] (Usefulness of topical skin compositions) Generally, the greater the amount of water incorporated into a topical skin composition, the greater the risk of a decrease in the activity of proteases and lipolytic enzymes. Since it is very difficult to prevent a decrease in the activity of proteases and lipolytic enzymes in a formulation containing a relatively large amount of water, in the development of a topical skin composition, it is often necessary to select a formulation containing a relatively small amount of water or substantially no water (for example, a facial cleansing powder). Although topical skin compositions formulated to be substantially water-free (e.g., facial cleansing powders) have achieved high user satisfaction with their efficacy, there is still room for improvement in terms of usability and convenience. For example, some users may consider facial cleansing powders to be inferior in usability and convenience to foam cleansers packed in pump formers or cream cleansers packed in tube-type containers.

[0124] This topical skin composition is extremely useful in that it can increase formulation flexibility, for example, because it can suppress a decrease in the activity of proteolytic enzymes and / or lipolytic enzymes, even in formulations that contain a relatively large amount of water as a base to be incorporated into the topical skin composition. From this perspective, one preferred embodiment of the present invention is a topical skin composition containing (A) protease and / or lipase, (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts, and (C) water, wherein the total content of the water (C) is 20% by mass or more, preferably 30 to 90% by mass, more preferably 35 to 85% by mass, and even more preferably 40 to 80% by mass, based on the total amount (100% by mass) of the topical skin composition. As in this embodiment, even when a relatively large amount of water is contained, the activity of the protease and / or lipase can be effectively suppressed, thereby allowing for greater flexibility in formulation than ever before.

[0125] Specifically, the present invention provides a skin cleansing composition containing, for example, (A) a protease and / or a lipolytic enzyme, (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts, and (C) water, wherein the total content of (C) water is 20 to 90% by mass relative to the total amount (100% by mass) of the skin cleansing composition, and the skin cleansing composition is filled in a foam-dispensing container. This embodiment achieves a high degree of compatibility between the excellent cleansing power of the protease and / or the lipolytic enzyme and ease of use and convenience, thereby providing users with a level of satisfaction not previously achieved with cleansing agents.

[0126] As described above, the skin cleansing composition filled in the foam-dispensing container is prepared appropriately using known methods using the above-mentioned components (A), (B), (C), "other components," etc. as raw materials.

[0127] In addition, the effect of suppressing the decrease in the enzymatic activity of proteases and lipolytic enzymes in this topical skin composition is that the addition of component (B) can suppress the decrease in enzymatic activity. This effect can be evaluated, but is not limited to, by comparing a formulation containing component (B) with a formulation excluding component (B) to confirm whether the addition of component (B) suppresses the decrease in enzymatic activity. Specifically, this can be evaluated, for example, by the method described in the Examples below.

[0128] <Enzyme activity stabilizer> In another embodiment of the present invention, the enzyme activity stabilizer of protease and / or lipase is also suitable. For example, the enzyme activity stabilizer of an external skin composition contains one or more active ingredients selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts. By incorporating the enzyme activity stabilizer of the embodiment of the present invention into the external skin composition, the activity of the enzyme contained in the external skin composition is stabilized.

[0129] Another embodiment of the present invention is a topical skin composition containing an enzyme activity stabilizer containing one or more active ingredients selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts, and a protease and / or a lipid-degrading enzyme.

[0130] <Enzyme activity stabilization method> Another embodiment of the present invention is also preferably provided as a method for stabilizing enzyme activity, for example, a method for stabilizing the enzymatic activity of (A) a protease and / or a lipolytic enzyme by allowing (A) a protease and / or a lipolytic enzyme and (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts to coexist in a composition for external use on skin. [Example]

[0131] EXAMPLES The present invention will be described in detail below based on examples, but the present invention is not limited to these. The units of content shown in the examples and formulation examples are % by mass.

[0132] Compositions (control, comparative examples 1-2, examples 1-6) having the formulations shown in Table 1 below were prepared by a conventional method. Specifically, disodium hydrogen phosphate and sodium dihydrogen phosphate dihydrate were dissolved in purified water to prepare a phosphate buffer of pH 6.2, and the remaining ingredients were then added and stirred to dissolve. The enzyme activity of these compositions was evaluated by the following method.

[0133] <Enzyme activity evaluation test> 20g of each composition was filled into a 20mL glass vial and stored at 40℃ for 2 weeks in the dark. The enzyme activity after storage was measured by changing the reaction time of the sample solution with the casein solution from 10 minutes to 30 minutes according to "Protease Potency Test Method 1" of the Quasi-drug Raw Materials Standards. The relative enzyme activity, with the enzyme activity of the control after storage set at 100, is shown in Figure 1.

[0134] [Table 1]

[0135] As shown in Table 1 and Figure 1, compared to the control, in Examples 1 to 6, in which inulin, inositol, seaweed extract, Geranium globulus extract, and one selected from the group consisting of hyaluronic acid and its salts were added, the enzyme activity was well maintained.

[0136] In addition, although it has been reported that sugars tend to function as stabilizers of enzyme activity, a tendency for enzyme activity to be inactivated was confirmed in Comparative Example 1, in which hydrolyzed hyaluronic acid was added as a sugar to the control, and in Comparative Example 2, in which isomerized sugar was added as a sugar to the control.

[0137] In Example 1, the "mass of (B) component per unit of (A) component (mass (g) of (B) component contained per gram of topical skin composition / number of units of (A) component (U))" is calculated as "2 (g) / 15,000 (U)." In addition, the "mass of (C) water per unit of (A) component (mass (g) of (C) water contained per gram of topical skin composition / number of units of (A) component (U))" in Example 1 is calculated as "97.78 (g) / 15,000 (U)."

[0138] Examples of formulations according to the present invention are given below.

[0139] <Formulation Example 1: Facial cleanser (foaming facial cleanser filled in a foam-dispensing container)> TIFF2025146083000002.tif109156

[0140] <Formulation example 2: Facial cleanser (facial cleansing powder)> TIFF2025146083000003.tif102155

[0141] <Formulation Examples 3 and 4: Facial cleanser (cream-type facial cleanser filled in a tube-type container)> TIFF2025146083000004.tif163149

Claims

1. (A) a proteolytic enzyme and / or a lipolytic enzyme, (B) one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts; A composition for external use on the skin comprising:

2. The mass of the component (B) per unit of the component (A) (the mass (g) of the component (B) contained in one unit g of the composition for external use on skin / the number of units (U) of the component (A)) is 1.0 × 10 -10 ~1.0 x 10 -1 The composition for external use on skin according to claim 1,

3. The mass of water (C) per unit of component (A) (mass of water (C) (g) / number of units of component (A) (U) contained in unit g of the composition for external use on skin) is 0 to 6.0 × 10 -2 The composition for external use on skin according to claim 1 or 2,

4. The mass of water (C) per unit of component (A) (mass of water (C) (g) / number of units of component (A) (U) contained in unit g of the composition for external use on skin) is 7.5 × 10 -4 ~6.0 x 10 -2 The composition for external use on skin according to claim 1 or 2,

5. 3. The topical skin composition according to claim 1, wherein the component (B) is one or more selected from the group consisting of inulin, Ascophyllum nodosum extract, and Geranium globulus extract.

6. A stabilizer for proteolytic enzymes and / or lipolytic enzymes in a topical skin composition, which contains as active ingredients one or more selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts.

7. A method for stabilizing the enzymatic activity of (A) a protease and / or a lipolytic enzyme by causing (A) a protease and / or a lipolytic enzyme to coexist with (B) one or more members selected from the group consisting of inulin, inositol, seaweed extract, Geranium globulus extract, and hyaluronic acid and its salts in a topical skin composition.

Images