Skin moisturizer

Abstract

To provide a novel use of grape skin for the effective use of grape juice residue to be discarded.SOLUTION: A skin moisturizer of the invention has been completed by finding the fact that grape skin has effects of inhibiting an increase in transepidermal water loss.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a skin moisturizer containing grape skin, and to a food or drink containing the skin moisturizer. [Background technology]

[0002] (Prior literature) An "oral water loss inhibitor containing ceramide extracted from rice and other plants" has been reported (Patent Document 1). A "cosmetic and / or oral ingestion composition containing polyphenols and ceramides" has been reported (Patent Document 2).

[0003] A "hyaluronidase inhibitor containing an extract of grape seeds and / or grape pomace as an active ingredient" has been reported (Patent Document 3). A composition has been reported that includes, "a composition comprising isolated dehydrated grape skins having a moisture content of 20% or less from the white grape variety Vitis vinifera for rectal administration or for oral administration as a gastroresistant delayed-release oral form for use in a method for preventively or therapeutically treating intestinal dysbiosis in a subject, wherein the intestinal dysbiosis is characterized by a decrease in the abundance and / or activity of butyrate-producing bacteria selected from the group consisting of Roseburia hominis and Subdoligranulum variabile, and the composition has a prebiotic effect of increasing the abundance and / or activity of the butyrate-producing bacteria in the intestine" (Patent Document 4).

[0004] The above prior art documents do not disclose or suggest a "skin moisturizer containing grape skin." [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent Publication No. 2023-53551 [Patent Document 2] Patent Publication No. 2023-59809 [Patent Document 3] Patent Publication No. 2000-026306 [Patent Document 4] Special Table 2020-521801 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a novel use of grape skins in order to effectively utilize discarded grape pomace. [Means for solving the problem]

[0007] As a result of extensive research to solve the above problems, the present inventors discovered that grape skin has the effect of suppressing an increase in the amount of water lost from the skin, and thus completed the skin moisturizing agent of the present invention.

[0008] That is, the present invention is as follows. [1] A skin moisturizer containing grape skin. [2] A food or beverage composition for moisturizing the skin, containing the skin moisturizer described in [1] above. [Effects of the Invention]

[0009] According to the present invention, it is possible to provide a skin moisturizing agent and a food or beverage composition for moisturizing skin containing the skin moisturizing agent. Furthermore, the skin moisturizing agent of the present invention can effectively utilize discarded grape pomace and is a highly safe natural material with a long history of use in food, so it is safe and highly useful when used in foods. [Brief explanation of the drawings]

[0010] [Figure 1] Measurement results of skin moisture loss. **p<0.01, t-test showed a significant difference for the control group compared to the untreated group. ##p<0.01, Dunnett's multiple comparison test showed a significant difference for the medium-dose and high-dose grape skin powder groups compared to the control group. DETAILED DESCRIPTION OF THE INVENTION

[0011] The present invention will be described in further detail below. The skin moisturizer of the present invention contains grape skin as an active ingredient.

[0012] (Grapes) The grapes used in the present invention belong to the genus Vitis in the family Vitaceae, and include European grapes (Vitis Vinifera) and American grapes (Vitis Labrusca), such as Niagara, Kyoho, Beni Fuji, Beni Izu, Rosario Bianco, and Rosario Rosso. Niagara grapes are preferred.

[0013] (grape juice) In the process of producing wine or grape juice, the juice is extracted from grapes using a press that can apply moderate pressure. When brewing wine, the juice is extracted in a way that minimizes crushing of the seeds and also controls the amount of undesirable components that are contained in the wine. Grape pomace contains seeds and skins containing unsqueezed components from the squeezing process.

[0014] (grape skins) The "grape skin" of the present invention can be obtained, for example, by a method including the following steps, but is not particularly limited thereto. (1) The grape pomace is dried under reduced pressure at a temperature of 80°C or less until the moisture content is 14% or less, preferably at 40 to 80°C, and more preferably at 60 to 80°C. After drying, the dried pomace containing the seeds and skins is stirred to crush only the skins, and then the seeds and skins are separated by sieving. (2) The separated pericarp is pulverized to a size of 1 mm or less (for example, 0.1 μm to 1 mm) to obtain a powder form. The "grape skin" of the present invention may also contain seeds that cannot be unavoidably separated in the above process and components derived from the seeds. The "grape skins" of the present invention do not require an extraction process from pomace. Furthermore, the "grape skin" of the present invention is preferably pre-fermented, since the polyphenol content of the skin is low before fermentation.

[0015] Examples of the components of the "grape skin" of the present invention include the following. Basic nutritional information (per 100g): Water 2.7g, Protein 11.6g, Fat 6.1g, Ash 7.4g, Carbohydrate 30.1g, Dietary fiber 42.1g, Sodium 2.5g Other ingredients ( / 100g): Polyphenols 3.7g, Oleanolic acid 1.7g Additionally, it has been confirmed that the "grape skin" components of the present invention are substantially free of ceramide (glucosylceramide). "Substantially free of ceramide" means that the amount of ceramide present is less than the amount that would have no effect. For example, the glucosylceramide content in dried grape skin powder can be 0.4% by mass or less, 0.3% by mass or less, 0.25% by mass or less, or 0.21% by mass or less. The glucosylceramide content can be measured by a known method, such as the method described in the literature "Nippon Shokuhin Kagaku Kogaku Kaishi Vol. 59, No. 1, 34-39 (2012)."

[0016] (Skin moisturizing effect) The skin moisturizing effect of the "skin moisturizer" of the present invention means effects such as the therapeutic and preventive effect on dry skin, the alleviation of symptoms of skin diseases, the protection effect on sensitive skin, the care effect on aging skin, the care effect after sunburn, the effect of adjusting the makeup base, and the improvement of wrinkles, due to the effect of suppressing the amount of water evaporation from the skin obtained in the examples below.

[0017] (in the form of a skin moisturizer) The skin moisturizing agent of the present invention can be formulated according to a conventional method, but is preferably formulated as an oral preparation.The dosage form may be a solid preparation or a liquid preparation.For example, tablets, granules, powders, capsules, emulsions, liquids, gels, syrups, slurries, suspensions, etc. may be mentioned. Furthermore, additives such as excipients can be added as needed during formulation. For example, excipients, binders, lubricants, disintegrants, surfactants, thickeners, coating agents, pH adjusters, antioxidants, sweeteners, preservatives, colorants, flavorings, etc. can be used alone or in combination depending on the purpose. The additives are not particularly limited, and examples include lactose, crystalline cellulose, calcium stearate, gelatin, glycerin fatty acid esters, xanthan gum, shellac, citric acid, sodium citrate, sodium ascorbate, and benzoic acid. The content of the additives is not particularly limited, and is, for example, 99.99 to 10% by mass, preferably 99.9 to 30% by mass, and more preferably 99 to 50% by mass, of the total amount of the additives.

[0018] The skin moisturizer of the present invention may be added to food and drink or food and drink compositions for skin moisturizing, and the food and drink may be in the form of liquid, gel, powder, solid, etc. Specific examples include, but are not limited to, beverages (tea, soup, etc.), seasonings (dressing, miso, etc.), processed meat and fish products (ham, sausage, etc.), bread, confectioneries (jelly, ice cream, etc.), dairy products (yogurt, cream, etc.), etc. In addition, the skin moisturizing agent of the present invention can also be used as a medicine, a quasi-drug, or a cosmetic.

[0019] Furthermore, the skin moisturizer of the present invention may be combined with other physiologically active substances or health food ingredients, such as green juice, health vinegar, royal jelly, propolis, blueberries, isoflavones, garlic, turmeric, enzymes, ginkgo biloba, maca, collagen, glucosamine, carnitine, ceramide, hyaluronic acid, collagen, elastin, GABA, CoQ10, vitamins, and minerals.

[0020] The grape skin contained in the skin moisturizer of the present invention is widely used as a food derived from natural products. In this respect, the intake amount of the skin moisturizer of the present invention (intake amount of grape skin powder) is not strictly limited. For example, the lower limit of the intake amount of grape skin powder is the minimum amount that can exert an effect according to the purpose, and the upper limit is based on a practical amount from the viewpoints of ease of intake, economy, etc., and the intake amount is usually 20 mg to 25 g, preferably 100 mg to 5 g per day for an adult. The intake period is preferably long-term, and it is desirable to take it continuously for one week or more, preferably one month or more, and more preferably three months or more. [Example]

[0021] The skin moisturizing agent of the present invention will be described in more detail below with reference to examples, but the skin moisturizing agent of the present invention is not limited thereto.

[0022] [Test sample] The test sample was prepared by drying grape juice residue (pomace), pulverizing only the skins by stirring, and separating the skin powder from the seeds using a sieve to obtain the skin powder. Grape variety used: Niagara Method of making juice residue: Pressing Drying conditions: reduced pressure, 60°C, moisture content 6% Dried peel crushing method: High-speed mixer (less than 1 mm) Peel and seed separation method: 2.36mm sieve The process for producing dried peel powder from the juice residue does not require an extraction step.

[0023] (Quantitative analysis of ceramides in dried grape skin powder) Quantitative analysis of the ceramide content of dried grape skin powder was carried out using the following conditions and equipment. Analysis method: Reference: "NipponShokuhin Kagaku Kogaku Kaishi Vol. 59, No. 1, 34~39 (2012)" Sample: 1 g of the above test sample was extracted several times using chloroform, ethanol, and water, and the dried lower layer was dissolved in a chloroform-methanol mixture to prepare the analytical solution. Equipment: HPLC Detector: Evaporative Light Scattering (ELS) detector Pure wheat-derived glucosylceramide was used as a standard for analysis. As a result of quantitative analysis, it was confirmed that the glucosylceramide content was 0.21% by mass.

[0024] [Evaluation of skin barrier function] An evaluation test was conducted using mice with induced dry skin. Hairless mice (Hos:HR-1, 4-week-old, male, Hoshino Laboratory Animal Care Facility) were acclimated for one week and then divided into groups as shown in Table 1 below using a stratified sequential randomization method based on skin water loss. After group allocation, the rats were divided into five groups: one group fed a normal diet (Labo MR Stock, manufactured by Nippon Nosan Kogyo Co., Ltd.) (untreated group), one group fed a special diet to induce dry skin (refined diet for HR-AD, manufactured by Nippon Nosan Kogyo Co., Ltd.) (control group), one group fed the special diet and 167 mg / kg / day of grape skin powder (low-dose grape skin powder group), one group fed the special diet and 1670 mg / kg / day of grape skin powder (medium-dose grape skin powder group), and one group fed the special diet supplemented with 5% by mass of grape skin powder and 1670 mg / kg / day of grape skin powder (high-dose grape skin powder group). The day of group allocation was designated day 0, and the rats were administered from day 1 to day 28.

[0025] [Table 1]

[0026] (Measurement of skin moisture loss) The amount of transepidermal water loss was measured using a Tewameter TM300 (manufactured by Courage+Khazaka electronic GmbH) on days 0 and 28. The measurement results for each group are shown in FIG. (statistical analysis) Data are expressed as mean ± standard error. Significant differences between the control and untreated groups were determined by F-test to test for homogeneity of variance. In the case of homogeneity of variance, a Student's t-test was used, and in the case of unequal variance, an Aspin-Welch t-test was used. The significance level for the F-test was set at a 5% abstention rate, and for the t-test, the significance levels were set at 5% and 1%. To determine significant differences between the control group and the grape skin powder-administered group, a test for homogeneity of variance was performed using the Bartlett method. If the variances were homogeneous, a one-way analysis of variance was performed, and if significant, the mean values ​​were compared using the Dunnett method. If the variances were unequal, a Kruskal-Wallis H test was performed, and if significant, the mean ranks were compared using the Dunnett method. The significance level for the Bartlett method, one-way analysis of variance, and Kruskal-Wallis H test was set at a 5% risk level, and for the Dunnett method, the significance levels were set at 5% and 1%. (Skin water loss measurement results) In the control group, an increase in skin moisture loss was observed from day 28 onwards, confirming that the special feed had induced a decrease in the skin's moisturising function. In contrast, the medium and high dose groups of grape skin dry powder showed significantly lower values ​​compared to the control group on day 28. It was shown that grape skin dry powder inhibited the increase in skin moisture loss in a dose-dependent manner and improved the decrease in moisturising ability.

[0027] (Prescription example) Tablets and green juice containing grape skin were produced according to the formulations in Tables 2 and 3 shown below in a conventional manner.

[0028] [tablet] Tablets were obtained according to a conventional method using the formulation shown in Table 2. Tablets containing the skin moisturizing agent of the present invention could be produced.

[0029] [Table 2]

[0030] [tablet] A green juice was obtained according to a conventional method using the formulation shown in Table 3. A green juice containing the skin moisturizing agent of the present invention was successfully produced.

[0031] [Table 3] [Industrial Applicability]

[0032] The present invention provides a skin moisturizing agent and a food or beverage composition for moisturizing the skin containing the skin moisturizing agent.

Claims

1. A skin moisturizer containing grape skin.

2. A skin moisturizing food or drink composition comprising the skin moisturizing agent according to claim 1.

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