Magnetic field changing device, sample analysis system, and sample analysis method

Abstract

To miniaturize a sample analysis system.SOLUTION: A sample analysis system includes: a magnet 101 that applies a magnetic field to a magnetic particle P bound to a labeling substance; a magnetic flux induction unit 120 composed of soft magnetic material; and a magnetic field change unit 110 that changes a magnetic field around the magnet 101. The magnetic flux induction unit 120 has a first surface facing an N-pole of the magnet 101 and a second surface facing an S-pole of the magnet 101. The first surface and the second surface are connected by a member of the magnetic flux induction unit 120. The magnetic field change unit 110 changes the magnetic field by creating a state in which the N-pole faces the first surface and a state in which the S-pole faces the second surface.SELECTED DRAWING: Figure 2

Description

[Technical Field]

[0001] The present invention relates to a magnetic field changing device, a sample analysis system, and a sample analysis method. [Background technology]

[0002] Sample analyzers used for immunological analysis and other analyses use a magnetic field to capture magnetic particles bound to the substance (sample) being measured, and measure the amount of light emitted by the chemiluminescent reaction to analyze the components in the sample. The photodetector that measures the amount of light emitted by the chemiluminescent reaction is affected by the magnetic field. Therefore, a large magnetic field (strong magnetic field) is required when capturing magnetic particles, and a small magnetic field (weak magnetic field) is required when measuring the amount of light emitted. For this reason, sample analyzers are equipped with a magnetic field switching device that switches the magnetic field on and off. A permanent magnet is used in the magnetic field switching device to generate the strong magnetic field. The magnetic field switching is achieved by a moving unit that moves the permanent magnet closer and further away.

[0003] An example of such a sample analyzer is disclosed in Patent Document 1. Patent Document 1 discloses an automatic analyzer comprising "a flow chamber through which a fluid containing magnetic particles bound to a labeling substance flows from a fluid inlet to a fluid outlet, magnetic trapping means for trapping the magnetic particles in the fluid introduced into the flow chamber, wherein the magnetic trapping means includes a magnet for applying a magnetic field, a working electrode and a counter electrode for applying a voltage to the magnetic particles trapped by the magnetic trapping means to cause them to emit light, a photodetector element for detecting the emission of light from the labeling substance on the magnetic particles trapped in the flow chamber, and restriction means for restricting the region in which the photodetector element detects the emission of light so as to detect the emission of light from the labeling substance on some of the magnetic particles trapped by the magnetic trapping means on the upstream side, the restriction means being a working electrode formed so as to be exposed to a portion on the upstream side of the inner wall of the flow chamber where the magnetic particles are trapped by the magnetic trapping means" (see claim 1). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent No. 6239243 Summary of the Invention [Problem to be solved by the invention]

[0005] The photodetector that measures the amount of light emitted is subject to a decrease in sensitivity due to the influence of a magnetic field, so a low magnetic field environment is required when measuring the amount of light emitted. For this reason, the magnetic field switching device is equipped with an arm that increases the distance between the photodetector and the magnet.

[0006] However, in the conventional configuration, a large volume is required over the range of motion of the arm, which leads to an increase in the size of the detection unit and restrictions on the arrangement of the flow channels.

[0007] The present invention has been made in view of the above background, and an object of the present invention is to make a sample analysis system smaller. [Means for solving the problem]

[0008] In order to solve the above-mentioned problems, the present invention comprises a magnet that applies a magnetic field to magnetic particles bound to a labeled substance, a magnetic flux induction unit made of a soft magnetic material, and a magnetic field change unit that changes the magnetic field around the magnet, wherein the magnetic flux induction unit has a first surface facing the north pole of the magnet and a second surface facing the south pole of the magnet, the first surface and the second surface being connected by a member of the magnetic flux induction unit, and the magnetic field change unit changes the magnetic field by bringing the north pole into a state facing the first surface and the south pole into a state facing the second surface. Other solutions will be described as appropriate in the embodiments. [Effects of the Invention]

[0009] According to the present invention, the sample analysis system can be made smaller. [Brief explanation of the drawings]

[0010] [Figure 1] 1 is a diagram showing a schematic configuration of a sample analyzing system according to a first embodiment. [Figure 2] FIG. 2 is an enlarged schematic view of a magnetic field change device and a detection unit. [Figure 3] FIG. 2 is a functional block diagram showing the configuration of a controller used in the sample analyzing system of the present embodiment. [Figure 4A] FIG. 10 is a schematic diagram showing a state in which a magnetic field is applied to a particle capture region. [Figure 4B] FIG. 10 is a schematic diagram showing a state in which no strong magnetic field is applied to a particle capture region. [Figure 5A] 10 is a diagram showing the positional relationship between a magnetic flux guide and a magnet in a magnetic field-on state. FIG. [Figure 5B] FIG. 1 is a diagram (part 1) showing the distribution of the magnitude of the measured magnetic flux density. [Figure 6A] 10 is a diagram showing the positional relationship between a magnetic flux guide and a magnet in a magnetic field-off state. FIG. [Figure 6B] FIG. 2 is a diagram (part 2) showing the distribution of the magnitude of the measured magnetic flux density. [Figure 7] FIG. 10 is a diagram showing analysis conditions. [Figure 8] FIG. 10 is a diagram showing the relationship between the position of a magnet and the magnetic flux density. [Figure 9A] FIG. 1 is a diagram (part 1) showing a specific example of a magnetic field change device according to the first embodiment. [Figure 9B] FIG. 2 is a diagram (part 2) showing a specific example of the magnetic field change device according to the first embodiment. [Figure 10] 10A and 10B are diagrams illustrating the movement of a magnet by a magnetic field change unit in a comparative example. [Figure 11] 10A and 10B are diagrams illustrating the movement of a magnet by a magnetic field change unit in this embodiment. [Figure 12] 3 is a flowchart showing the steps of a sample analysis method performed by the sample analysis system. [Figure 13A] FIG. 10 is a diagram (part 1) showing the configuration of a magnetic field change device according to a second embodiment. [Figure 13B]FIG. 10 is a diagram (part 2) showing the configuration of the magnetic field change device in the second embodiment. [Figure 14A] FIG. 10 is a diagram (part 1) showing the configuration of a magnetic field change device according to the third embodiment. [Figure 14B] FIG. 10 is a diagram (part 2) showing the configuration of the magnetic field change device in the third embodiment. [Figure 15A] FIG. 10 is a diagram (part 1) showing the configuration of a magnetic field change device according to a fourth embodiment. [Figure 15B] FIG. 10 is a diagram (part 2) showing the configuration of the magnetic field change device according to the fourth embodiment. [Figure 16A] FIG. 3 is a diagram showing the configuration of a magnetic flux guide portion used in the first to third embodiments. [Figure 16B] 10A and 10B are diagrams illustrating a configuration of a magnetic flux guide portion used in the fourth embodiment. [Figure 17A] FIG. 1 is a diagram (part 1) showing a configuration example of a magnetic flux guide portion used in the first to third embodiments. [Figure 17B] FIG. 10 is a diagram (part 2) showing an example of the configuration of the magnetic flux guide portion used in the first to third embodiments. [Figure 17C] FIG. 10 is a diagram (part 3) showing an example of the configuration of the magnetic flux guide portion used in the first to third embodiments. [Figure 18A] FIG. 10 is a diagram (part 1) showing a configuration example of a magnetic flux guide portion used in the fourth embodiment. [Figure 18B] FIG. 13 is a diagram (part 2) showing a configuration example of a magnetic flux guide portion used in the fourth embodiment. DETAILED DESCRIPTION OF THE INVENTION

[0011] Next, modes for carrying out the present invention (referred to as "embodiments") will be described in detail with reference to the drawings as appropriate.

[0012] [First embodiment] Fig. 1 is a diagram showing a schematic configuration of a sample analysis system 1 according to the first embodiment. Fig. 2 is an enlarged schematic view of a magnetic field change device 100 and a detection unit 200.

[0013] In this embodiment, an immunoanalysis device will be described as an example of a sample analysis system 1. This embodiment is not limited to immunoanalysis, and can be applied to any sample analysis system 1 that captures magnetic particles P by changing the magnitude of the magnetic field (distribution of magnetic flux density). In other words, the sample analysis system 1 shown in this embodiment can be used for analyzers for DNA, biochemistry, etc. Furthermore, the embodiments shown below are not limited to shapes, dimensions, numbers, etc., and can be modified within the scope of the gist thereof, and multiple embodiments, including those described below, can be combined. Furthermore, part of the configuration of one embodiment can be replaced with the configuration of another embodiment.

[0014] In this embodiment, the magnitude of the magnetic field is expressed numerically using the term magnetic flux density.

[0015] The sample analysis system 1 includes a magnetic field change device 100 (see FIG. 2), a detection section 200 (see FIG. 2), a controller 300, and a voltage application section 401. The sample analysis system 1 further includes a sipper nozzle 411, a pump 412, an arm 415, a reaction unit 511, a suspension container 512, a washing liquid container 513, a buffer solution container 514, a washing section 515, and a waste liquid container 516.

[0016] (Detection unit 200) As shown in FIG. 2, the detection unit 200 is composed of a photodetector 201, a flow channel 210, a reaction field electrode 221, and a counter electrode 222. The flow channel 210 is formed by a flow channel top wall 211, a flow channel bottom wall 212, and a side wall 213 (see FIG. 9A). The flow channel 210 may be configured inside a flow cell (not shown). Magnetic particles P bound to a labeled substance flow through the flow channel 210. The flow channel top wall 211 is preferably made of a transparent material so that the photodetector 201 can detect the wavelength of light emitted by the labeled substance bound to the magnetic particles P around the reaction field electrode 221. The flow channel top wall 211 is preferably made of, for example, glass, plastic, or the like.

[0017] A reaction field electrode 221 is installed around the lower part of the flow channel 210. A counter electrode 222 is installed around the opposite side of the reaction field electrode 221, sandwiching the flow channel 210. Furthermore, the reaction field electrode 221 and the counter electrode 222 are connected to a voltage application unit 401 via signal lines L11 and L12. The voltage application unit 401 is connected to the controller 300 via signal line L2.

[0018] The materials for reaction field electrode 221 and counter electrode 222 are, for example, gold, platinum, palladium, tungsten, iridium, nickel, alloys thereof, carbon materials, etc. Alternatively, reaction field electrode 221 and counter electrode 222 may be formed by plating, sputtering, or the like, the above-mentioned materials on a base material such as titanium.

[0019] The reaction field electrode 221 is preferably planar for ease of luminescence measurement. Furthermore, it is desirable that the reaction field electrode 221 be provided on the bottom surface of the flow channel 210. However, the reaction field electrode 221 may be provided on other surfaces (surfaces other than the bottom surface) within the flow channel 210, or on multiple surfaces arranged three-dimensionally. Furthermore, the reaction field electrode 221 does not necessarily have to be planar as long as it is suitable for capturing the magnetic particles P or for electrochemiluminescence of the labeled substance bound to the magnetic particles P. That is, the reaction field electrode 221 may be configured in a linear shape, or a combination of a linear shape and a planar shape. The counter electrode 222 may have any configuration or shape that can generate a voltage in the flow channel 210 in combination with the reaction field electrode 221. That is, the counter electrode 222 may be configured in a linear shape, a planar shape, or a combination thereof.

[0020] (Magnetic field change device 100) As shown in FIG. 2, the magnetic field change device 100 is made up of a magnet 101, a magnetic flux guide unit 120 made of a soft magnetic material, and a magnetic field change unit 110.

[0021] Magnet 101, which is a permanent magnet, applies a magnetic field to magnetic particles P. Specifically, magnet 101 generates a magnetic field for attracting magnetic particles P to particle capture region 231 above reaction field electrode 221. Reaction field electrode 221 and counter electrode 222 are provided so that particle capture region 231 is located directly below photodetector 201. By using a permanent magnet for magnet 101, the configuration of magnetic field change device 100 can be simplified.

[0022] The magnetic field change unit 110 changes the magnetic field around the magnet 101. Specifically, the magnetic field change unit 110 moves the magnet 101 to a position close to the flow channel 210 (referred to as a magnetic field-on position) and a position away from the flow channel 210 (referred to as a magnetic field-off position). The magnetic field change unit 110 is connected to the controller 300 via a signal line L6. That is, the controller 300 controls the magnetic field change unit 110. The magnetic flux induction unit 120 is disposed so as to surround the magnet 101 when the magnet 101 is in a position away from the flow channel 210 (magnetic field-off position). The magnetic flux induction unit 120 will be described later. The magnetic flux induction unit 120 changes the magnitude (distribution of magnetic flux density) of the magnetic field generated from the magnet 101 and affecting the magnetic particles P and the photodetector 201. The magnetic flux induction unit 120 is made of any one of iron, silicon steel, permalloy, permendur, a magnetic alloy, and soft ferrite.

[0023] In this way, the magnet 101 is provided to keep the magnetic particles P directly below the photodetector 201 .

[0024] Voltage application unit 401 is controlled by controller 300. In response to a command from controller 300 to voltage application unit 401, voltage application unit 401 applies a voltage between reaction field electrode 221 and counter electrode 222 inside flow channel 210. When voltage application unit 401 applies a voltage, the labeled substance bound to magnetic particles P adsorbed to the upper part of reaction field electrode 221 electrochemically emits light.

[0025] The photodetector 201 constituting the detection unit 200 detects light emitted by the labeled substance. Specifically, the photodetector 201 detects the wavelength of light emitted by the labeled substance bound to the magnetic particles P in the vicinity of the reaction field electrode 221. For example, a camera or a photomultiplier tube can be used as the photodetector 201. The photodetector 201 is operated by a signal transmitted from the controller 300.

[0026] Flow path 210 formed inside the flow cell is connected to sipper nozzle 411 and pump 412 through tubes T1 and T2. Sipper nozzle 411 is movable by arm 415, and suspension container 512 to cleaning unit 515 are installed within the range of movement of sipper nozzle 411.

[0027] A valve 413 is provided in a tube T2 connecting the flow path 210 and the pump 412. The pump 412 and the valve 413 are connected to the controller 300 via signal lines L5 and L3. Furthermore, the flow path 210 continues to a waste liquid container 516 via a valve 414 and a tube T3.

[0028] The controller 300 is connected to the photodetector 201, the voltage application unit 401, the valves 413 and 414, the pump 412, the magnetic field change unit 110, and the arm 415 via independent signal lines L1 to L6. This allows the controller 300 to control the photodetector 201, the voltage application unit 401, the valves 413 and 414, the pump 412, the magnetic field change unit 110, and the arm 415 independently.

[0029] The sample to be analyzed is a substance of biological origin, such as serum or urine. When the sample is serum, the specific component to be analyzed may be, for example, a tumor marker, an antibody, an antigen-antibody complex, or a single protein. In the following explanation, the component to be identified by analysis (specific component) is assumed to be TSH (thyroid stimulating hormone). However, substances other than TSH may also be used in the analysis.

[0030] In the reaction unit 511, as a pretreatment step, the sample to be analyzed is mixed with a bead solution and a reagent.

[0031] The suspension container 512 contains a mixture of a sample to be analyzed, a bead solution, and a reagent, which is then reacted at a constant temperature (e.g., 37°C) for a certain period of time as a pretreatment step. The bead solution is a solution in which magnetic particles P, which are particulate magnetic substances embedded in a matrix material such as polystyrene, are dispersed in a buffer solution. Streptavidin, which can bind to biotin, is bound to the surface of the matrix material. The reagent contains a substance that binds the magnetic particles P to the specific component TSH in the sample. The reagent also contains an anti-TSH antibody whose end is biotinylated. Different reagents are used depending on the type of specific component to be analyzed; for example, immunoglobulin, antigen, antibody, or other biological substance may be used as the reagent. The solution containing the magnetic particles P and the sample is referred to as a suspension.

[0032] The cleaning liquid container 513 contains a cleaning liquid for cleaning the inside of the flow channel 210 and the tube T1.

[0033] The buffer solution container 514 contains a buffer solution.

[0034] The waste liquid container 516 contains the liquid discharged from the pump 412 .

[0035] The flow channel 210 near the photodetector 201 preferably has a rectangular cross-sectional shape as shown in FIG. 1 to facilitate capture of the magnetic particles P. The shape of the flow channel 210 is defined by the length (path length) along the flow of the magnetic particles P (suspension), and the thickness (vertical direction) and width (horizontal direction) of the cross section perpendicular to the flow. The flow channel 210 near the photodetector 201 is preferably formed such that the path length is 2 to 20 times the greater of the thickness and width. This configuration of the flow channel 210 ensures a sufficient path for the magnetic particles P to flow. Furthermore, the above-described shape of the flow channel 210 allows the magnetic particles P in the fluid to spread within the flow channel 210. The magnetic particles P are then more easily adsorbed by the particle capture region 231 configured above the reaction field electrode 221 provided on the bottom surface of the flow channel 210.

[0036] The distribution of magnetic particles P adsorption inside flow channel 210 is determined by the magnetic force from magnet 101 installed near flow channel 210 and the drag force caused by the fluid flow. The magnetic flux density inside flow channel 210 is preferably about 0.1 to 0.5 T. The flow velocity of the fluid at that time is preferably about 0.05 to 0.10 m / s.

[0037] The particles used as the magnetic particles P are preferably the following particles. (A1) Particles that exhibit paramagnetism, superparamagnetism, ferromagnetism, or ferrimagnetism. (A2) Particles exhibiting paramagnetic, superparamagnetic, ferromagnetic, or ferrimagnetic properties encapsulated in materials such as synthetic polymer compounds (polystyrene, nylon, etc.), natural polymers (cellulose, agarose, etc.), and inorganic compounds (silica, etc.).

[0038] The particle size of the magnetic particles P is preferably in the range of 0.01 to 200 μm, specifically in the range of 1 to 10 μm. The specific gravity of the magnetic particles P is preferably 1.3 to 1.5. By using magnetic particles P with such specifications, the magnetic particles P are less likely to settle in the liquid and are more likely to be suspended. Furthermore, a substance that has the property of specifically binding to the analyte (sample), such as an antibody that has the property of specifically binding to an antigen, is bound to the surface of the magnetic particles P.

[0039] The labeling substance is preferably a substance as shown below. Specifically, when the labeling substance has an appropriate structure as shown below, the labeling substance specifically binds to the analyte (sample) and emits light due to the appropriate structure.

[0040] (B1) Labeling substances used in fluorescent immunoassays, such as antibodies labeled with fluorescein isothiocyanate. (B2) Labeling substances used in chemiluminescent immunoassays, such as antibodies labeled with acridinium esters. (B3) Labeling substances used in chemiluminescent enzyme immunoassays, such as antibodies labeled with chemiluminescent enzymes that use luminol or adamantyl derivatives as luminescent substrates.

[0041] The above is an example of the configuration of the sample analysis system 1.

[0042] (controller 300) FIG. 3 is a functional block diagram showing the configuration of the controller 300 used in the sample analyzing system 1 in this embodiment.

[0043] The controller 300 is configured by, for example, a PC (Personal Computer), etc. The controller 300 includes at least a memory 301, a calculation device 302, a storage device 303, and a communication device 304.

[0044] The memory 301 is configured by a RAM (Random Access Memory) or the like.

[0045] The arithmetic device 302 is configured with a CPU (Central Processing Unit), a GPU (Graphic Processing Unit), and the like.

[0046] The storage device 303 is configured from a hard disk drive (HDD), a solid state drive (SSD), or the like.

[0047] Then, a program stored in the storage device 303 is loaded into the memory 301. The loaded program is then executed by the arithmetic device 302. In this way, various functions of the controller 300 are realized.

[0048] The communication device 304 acquires information from the photodetector 201, the voltage application unit 401, the valves 413 and 414, the pump 412, the magnetic field change unit 110, and the arm 415, and transmits information.

[0049] (change in magnetic field) Next, the change in the magnetic field around the particle capture region 231 will be described with reference to FIGS. 4A and 4B.

[0050] Fig. 4A is a schematic diagram showing a state (ON state) in which a magnetic field is applied to particle capture region 231, and Fig. 4B is a schematic diagram showing a state (OFF state) in which no strong magnetic field is applied to particle capture region 231. Note that the same components as those shown in Figs. 1 and 2 are denoted by the same reference numerals and descriptions thereof will be omitted.

[0051] The magnetic flux guider 120 is placed at a magnetic field off position, which is a predetermined distance away from the flow channel 210. When the magnet 101 is in a position close to the flow channel 210 (referred to as a magnetic field on position) as shown in Fig. 4A, the magnet 101 is outside the magnetic flux guider 120, and therefore a strong magnetic field acts on the flow channel 210. This allows the magnetic particles P flowing through the flow channel 210 to be captured by the magnetic field.

[0052] In this embodiment, the magnetization direction A2 of the magnet 101 is defined as the direction from the south pole 101b to the north pole 101a. As shown in Fig. 4A, the magnetization direction A2 of the magnet 101 is parallel to the bottom surface (flow path bottom wall 212) of the flow path where the magnetic particles P are captured by the magnetic field generated by the magnet 101. With this configuration, a large number of magnetic particles P can be captured.

[0053] When detecting luminescence from the labeling substance by photodetector 201, magnetic field change unit 110 moves magnet 101 to a magnetic field off position as shown in FIG. 4B to avoid the influence of the magnetic field on photodetector 201. At this time, magnet 101 fits into magnetic flux guide unit 120. As a result, as shown in FIG. 4B, most of the magnetic flux MF (magnetic field) generated from magnet 101 passes through the soft magnetic material that makes up magnetic flux guide unit 120. Therefore, the magnitude of the magnetic field acting on photodetector 201 can be reduced.

[0054] In this way, magnetic flux induction unit 120 that induces magnetic flux MF is arranged to cover the periphery of magnet 101 when magnet 101 is in the magnetic field off position. Specifically, in the state of FIG. 4B , magnetic flux induction unit 120 has a first surface 122 (see FIG. 4A) that faces north pole 101a (see FIG. 4A) of magnet 101. Magnetic flux induction unit 120 also has a second surface 123 (see FIG. 4A) that faces south pole 101b (see FIG. 4A) of magnet 101. First surface 122 and second surface 123 are connected by a member of magnetic flux induction unit 120.

[0055] Supplementally, magnetic flux guider 120 includes a first portion and a second portion facing the first portion, as well as a third portion (member) connecting the first portion and the second portion. First surface 122 is provided on the first portion, and second surface 123 is provided on the second portion, facing each other. In this embodiment, magnetic flux guider 120 has a concave cross-sectional shape, and the third portion connecting the first portion and the second portion forms a magnetic path (the cross-sectional shape may be U-shaped, C-shaped, or the like).

[0056] With this configuration, when the magnet 101 is housed (FIG. 4B) in the housing 121 (see FIG. 4A), most of the magnetic flux MF generated from the magnet 101 passes through the soft magnetic material that constitutes the magnetic flux guider 120. As described above, the magnitude of the magnetic field acting on the photodetector 201 can be reduced. This makes it possible to change the magnitude of the magnetic field over a shorter travel distance than with conventional magnetic field change devices 100. This allows the configuration of the magnetic field change device 100 to be made more compact.

[0057] In this way, under the control of the controller 300, the magnetic field change unit 110 changes the magnetic field by switching the state in which the N pole 101a faces the first surface 122 and the S pole 101b faces the second surface 123. The state in which the N pole 101a faces the first surface 122 and the S pole 101b faces the second surface 123 is defined as a first state. The state in which the N pole 101a does not face the first surface 122 and the S pole 101b does not face the second surface 123 is defined as a second state. The magnetic field change unit 110 then switches between the first state and the second state, thereby changing the magnetic field around the magnet 101.

[0058] Next, the change in the magnetic field caused by the magnetic field change device 100 will be described with reference to FIGS. 5A to 8. FIG.

[0059] Fig. 5A is a diagram showing the positional relationship between magnetic flux induction unit 120 and magnet 101 in a magnetic field-on state. Fig. 5B is a diagram showing the distribution of the magnitude of magnetic flux density (state of magnetic field) analyzed in the state shown in Fig. 5A. Fig. 6A is a diagram showing the positional relationship between magnetic flux induction unit 120 and magnet 101 in a magnetic field-off state. Fig. 6B is a diagram showing the distribution of the magnitude of magnetic flux density (state of magnetic field) analyzed in the state shown in Fig. 6A.

[0060] The magnetic field on state refers to a state in which a strong magnetic field is applied to the flow channel 210 by the magnet 101. The magnetic field off state refers to a state in which a weaker magnetic field than that in the magnetic field on state is applied to the flow channel 210.

[0061] Furthermore, Fig. 7 is a diagram showing the analysis conditions. Note that magnetic flux guide unit 120 in Fig. 7 shows the state of magnetic flux guide unit 120 as viewed from the direction of the white arrow shown in Fig. 5A and Fig. 6A.

[0062] In this analysis, magnet 101 was made of N49M and measured 3 x 6 x 4 mm. The magnetization direction A2 of magnet 101 was the x-axis direction in Figure 7. The material of magnetic flux guider 120 was ferrite. The external dimensions of magnetic flux guider 120 were 24 x 20 x 12 mm.

[0063] 7, in this analysis, the position 201a of the light incident surface of the photodetector 201 is set to be 10 mm (reference symbol D1) above the top surface of the magnet 101 when the magnetic field is on. Furthermore, the distance (reference symbol D2) between the top surface position of the magnet 101 and the top surface position of the magnetic flux guider 120 when the magnetic field is on is 10 mm. Note that "upper" refers to the positive direction of the z axis in FIG. 7. In other words, as shown in FIG. 7, the side of the photodetector 201 is defined as the upper side, and the side of the magnetic flux guider 120 is defined as the lower side. Note that the arrow A3 in FIG. 7 indicates the direction of movement of the magnet 101.

[0064] 5A and 6A, magnetic flux induction section 120 has a U-shape and has storage section 121, which is a space for storing magnet 101, inside.

[0065] As shown in Fig. 5A, in the magnetic field on state, magnet 101 is not contained in storage section 121 of magnetic flux guide section 120. In this state, the magnetic field (distribution of the magnitude of magnetic flux density) around magnet 101 is as shown in Fig. 5B. Fig. 5B shows that a strong magnetic field is applied to flow channel 210 (particle capture region 231), and furthermore, the magnetic field extends to photodetector 201.

[0066] As shown in FIG. 6A, in the magnetic field off state, magnet 101 is accommodated in storage section 121 (see FIG. 5A) of magnetic flux induction section 120. As a result, as described above, most of the magnetic flux MF (see FIGS. 4A and 4B) generated from magnet 101 passes through the interior of magnetic flux induction section 120. In the magnetic field off state shown in FIG. 6A, the magnetic field (distribution of the magnitude of magnetic flux density) around magnet 101 is as shown in FIG. 6B. As shown in FIG. 6B, there is almost no magnetic field outside magnetic flux induction section 120. In particular, FIG. 6B shows that there is almost no effect of the magnetic field around photodetector 201. The relationship between the gradation in FIG. 6B and the magnitude of magnetic flux density is as shown in the legend in FIG. 5B.

[0067] 5A and 6A, magnetic field change unit 110 moves magnet 101 in and out of storage section 121 of magnetic flux induction unit 120. With this configuration, the magnetic field generated by magnet 101 can be changed with a simple configuration.

[0068] 8 is a diagram showing the relationship between the position of magnet 101 and the magnetic flux density. With reference to FIG. 8, the movement distance of magnet 101 in the vertical direction and the magnitude of the magnetic flux density at position 201a on the light incident surface of photodetector 201 are evaluated.

[0069] 8, the horizontal axis represents the distance traveled by magnet 101. The distance traveled by magnet 101 is defined as the distance traveled by the top surface of magnet 101, with the position in FIG. 7 being "0." The vertical axis in FIG. 8 represents the magnetic flux density (unit: T) at position 201a (see FIG. 7) on the light incident surface of photodetector 201.

[0070] In addition, in FIG. 8, the dashed line B1 indicates the value of the magnetic flux density at which the photodetector 201 is not affected, and its magnitude is approximately 50 μT.

[0071] 8, curve C1 shows the change in magnetic flux density with the movement distance of the magnet 101 when the magnetic field change device 100 of this embodiment is used. Curve C2 shows the change in magnetic flux density with the movement distance of the magnet 101 when the magnetic field change device 100 of the comparative example is used. The comparative example is a magnetic field change device that is not provided with a magnetic flux induction unit 120.

[0072] 8, in the comparative example (curve C2), magnet 101 needs to move 42 mm or more around photodetector 201 to reach a magnetic flux density (50 μT: dashed line B) at which the sensitivity of photodetector 201 is not reduced. In contrast, in this embodiment (curve C1), the magnetic flux density (50 μT: dashed line B) at which the sensitivity of photodetector 201 is not reduced is reached with a movement distance of 15 mm or less. This is because, as described above, when magnet 101 moves to the magnetic field off position, magnet 101 fits into storage portion 121 (see FIG. 5A) of magnetic flux guide unit 120, and therefore most of the magnetic flux generated from magnet 101 passes through the interior of magnetic flux guide unit 120 and is not generated outside magnetic flux guide unit 120.

[0073] (Specific example of magnetic field change device 100) Next, a specific example of the magnetic field changing device 100 will be described with reference to FIGS. 9A and 9B.

[0074] 9A and 9B are diagrams showing a specific example of the magnetic field change device 100 according to the first embodiment. FIG. 9A shows a state in which the magnet 101 is positioned in the magnetic field-on position. FIG. 9B shows a state in which the magnet 101 is positioned in the magnetic field-off position. In FIGS. 9A and 9B, the same components as those in FIGS. 4A and 5B are denoted by the same reference numerals, and their description will be omitted. In FIGS. 9A and 9B, the magnetic particles P are assumed to flow from the back of the paper to the front of the paper.

[0075] 9A and 9B, the magnetic field change unit 110a includes an arm unit 111. A magnet 101 is provided at one end of the arm unit 111. A rotating unit 112 that rotates the arm unit 111 is provided at the other end of the arm unit 111. By rotating the rotating unit 112 (indicated by the double-headed arrow in FIGS. 9A and 9B), the magnet 101 can move between the magnetic field-on position in FIG. 9A and the magnetic field-off position in FIG. 9B.

[0076] Next, with reference to FIGS. 10 and 11, the difference between this embodiment and a comparative example in which the magnetic field change portion 110a has the arm portion 111 as shown in FIGS. 9A and 9B will be described.

[0077] Fig. 10 is a diagram showing the movement of magnet 101 by magnetic field change unit 110a in a comparative example, and Fig. 11 is a diagram showing the movement of magnet 101 by magnetic field change unit 110a in this embodiment. Note that the length of arm unit 111 is assumed to be the same in Fig. 10 and Fig. 11.

[0078] 10 and 11, the magnetic field change section 110a has the same configuration as that shown in Figures 9A and 9B. In Figures 10 and 11, the dashed lines indicate the magnetic field on positions, and the solid lines indicate the magnetic field off positions.

[0079] In the example shown in FIG. 10, in order to prevent the photodetector 201 from being affected by the magnetic field of the magnet 101, the arm 111 needs to be rotated by approximately 90° by the rotating part 112.

[0080] In contrast to this, in the example shown in FIG. 11, the influence of the magnetic field on the photodetector 201 can be reduced by simply rotating it by approximately 11.8°.

[0081] (flowchart) Next, a description will be given of the operation of the sample analysis system 1 of this embodiment. One analysis is considered to be one cycle, and the basic operating state of the device is to perform the cycle multiple times in succession.

[0082] Figure 12 is a flowchart showing the steps of the sample analysis method performed by the sample analysis system 1. Figures 1, 2 and 4A will be referenced as appropriate.

[0083] One cycle of sample analysis consists of a suspension aspiration period (S1), a magnetic particle capture period (S2), a detection period (S3), a washing period (S4), a reset period (S5), and a preliminary aspiration period (S6). One cycle starts when the suspension container 512 containing the suspension treated in the reaction unit 511 is set in a predetermined position.

[0084] During the suspension suction period (S1), controller 300 sets valve 413 to an open state and sets valve 414 to a closed state. Then, arm 415 is operated in response to a signal transmitted from controller 300. The operation of arm 415 causes sipper nozzle 411 to be inserted into suspension container 512. Subsequently, pump 412 performs a suction operation of a fixed amount in response to a signal transmitted from controller 300. This operation causes the suspension contained in suspension container 512 to be introduced into tube T1 via sipper nozzle 411.

[0085] Then, once the suspension has been introduced into the inside of tube T1, controller 300 temporarily stops pump 412. Then, controller 300 operates arm 415 to insert sipper nozzle 411 into cleaning unit 515. Sipper nozzle 411 is cleaned as it passes through cleaning unit 515. Incidentally, cleaning of sipper nozzle 411 is separate from cleaning performed during a cleaning period, which will be described later.

[0086] During the magnetic particle adsorption period (S2), which is the magnetic particle capture step, the magnetic field change unit 110 is operated by a signal transmitted from the controller 300. As a result, the magnet 101 moves to the vicinity of the particle capture region 231 at the bottom of the flow channel 210 (magnetic field on position). Thereafter, the pump 412 is operated by a signal transmitted from the controller 300. At this time, the pump 412 sucks the suspension at a constant speed. As a result, the suspension present inside the tube T1 flows inside the flow channel 210. A magnetic field generated by the magnet 101 is present on the wall 11 of the flow channel 210 and the bottom wall 212 of the flow channel. Therefore, the magnetic particles P contained in the suspension are attracted toward the magnet 101 by the magnetic field and are captured (adsorbed) in the particle capture region 231.

[0087] In the magnetic particle adsorption period (S2), the magnetic particles P are captured by the magnetic field generated by the magnet 101.

[0088] After the magnetic particles P have been captured for a certain period of time, the controller 300 stops the pump 412 from sucking the suspension.

[0089] During the detection period (S3), which is the detection step, the controller 300 operates the magnetic field change unit 110 to move the magnet 101 to a position (magnetic field off position) away from the flow channel 210. Subsequently, a signal is sent from the controller 300 to operate the photodetector 201 and apply voltage to the reaction field electrode 221 and the counter electrode 222. As a result, with the magnetic particles P held above the reaction field electrode 221, the photodetector 201 receives light emitted from the labeled substance bound to the magnetic particles P.

[0090] In this way, during the detection period (S3), after the magnetic particle adsorption period (S2), the magnetic field change unit 110 causes the N pole 101a to face the first surface 122 and the S pole 101b to face the second surface 123. Then, detection is performed by the photodetector 201.

[0091] Since the pump 412 stops sucking the suspension before the detection period, the magnetic particles P continue to remain in the particle capture region 231 even if the magnetic field from the magnet 101 disappears (decreases).

[0092] In this way, the luminescence intensity from the labeling substance is measured. The detected luminescence intensity is sent to the controller 300 as a signal.

[0093] After the measurement has been performed for a certain period of time, controller 300 stops the application of voltage to reaction field electrode 221 and counter electrode 222, and stops the operation of photodetector 201. Furthermore, during the detection period, controller 300 operates arm 415 to insert sipper nozzle 411 into cleaning section 515.

[0094] During the cleaning period (S4), the controller 300 uses the pump 412 to suck in the cleaning liquid. As a result, the cleaning liquid sucked from the cleaning liquid container 513 passes through the inside of the flow path 210. At this time, since the magnet 101 is away from the flow path 210 (located in the magnetic field off position), the magnetic particles P are not retained in the particle capture region 231 on the reaction field electrode 221 and are washed away together with the cleaning liquid.

[0095] In the reset period (S5), the controller 300 closes the valve 413, opens the valve 414, and causes the pump 412 to perform a discharge operation. As a result, the liquid inside the pump 412 is discharged into the waste liquid container 516.

[0096] During the preliminary suction period (S6), the controller 300 opens the valve 413 and closes the valve 414. Then, the controller 300 operates the arm 415 to move the sipper nozzle 411. As a result, the controller 300 inserts the sipper nozzle 411 into the buffer solution container 514. Thereafter, the controller 300 operates the pump 412 to aspirate the buffer solution from the buffer solution container 514. As a result, the inside of the tube T1 and the flow path 210 is filled with the buffer solution. After the preliminary suction period, the next cycle can be executed.

[0097] According to the first embodiment, the magnetic field change device 100 can be miniaturized without causing a decrease in the sensitivity of the photodetector 201. This makes it possible to miniaturize the unit formed by the magnetic field change device 100 and the detection section 200 and improve the degree of freedom in the configuration of the flow channel 210. By miniaturizing the unit formed by the magnetic field change device 100 and the detection section 200, the sample analysis system 1 can also be miniaturized.

[0098] Furthermore, by configuring the arm portion 111 to rotate by the rotating portion 112, the magnetic field change device 100 can be configured with a simple structure.

[0099] Furthermore, in the first embodiment, the magnetic field change unit 110 (110a) is configured to move the magnet 101 in a direction perpendicular to (i.e., up and down) the bottom surface of the flow channel (flow channel bottom wall 212) where the magnetic particles P are captured by the magnetic field generated by the magnet 101. The flow channel bottom surface is the surface of the flow channel bottom wall 212 that faces the flow channel 210, and corresponds to the lower surface of each of the surfaces that make up the flow channel 210. With this configuration, the magnetic field change device 100 can be configured simply.

[0100] [Second embodiment] 13A and 13B are diagrams showing the configuration of a magnetic field change device 100 in the second embodiment. FIG. 13A shows the case where the magnet 101 is in the magnetic field-on position, and FIG. 13B shows the case where the magnet 101 is in the magnetic field-off position. In addition, in FIGS. 13A and 13B, the same components as those in FIGS. 4A and 4B are denoted by the same reference numerals, and their description will be omitted. In addition, in FIGS. 13A and 13B, the flow path 210 and the magnetic flux guide 120 are shown as cross-sectional views.

[0101] The magnetic particles P move in the flow channel 210 from the back side of the paper to the front side of the paper.

[0102] 13A and 13B includes an arm 111, one end of which is provided with a magnet 101. The other end of the arm 111 is provided with a solenoid 113 that moves the arm 111 using a solenoid. The arm 111 has an L-shape. The solenoid 113 moves the arm 111 in the up and down direction using a solenoid (electromagnet) (as indicated by the double-headed arrow in FIGS. 13A and 13B).

[0103] According to the example shown in FIGS. 13A and 13B, the arm portion 111 and the magnet 101 are moved by the solenoid (electromagnet) of the solenoid portion 113, so that the magnet 101 can be moved quickly.

[0104] [Third embodiment] Next, a third embodiment of the present invention will be described with reference to FIGS. 14A and 14B.

[0105] 14A and 14B are diagrams showing the configuration of a magnetic field change device 100 according to the third embodiment.

[0106] In the first and second embodiments, an example is shown in which the magnet 101 moves in the vertical direction. The vertical direction means a direction perpendicular to the channel bottom (channel bottom wall 212) where the magnetic particles P are captured by the magnetic field generated by the magnet 101. In contrast, in the third embodiment, an example is shown in which the magnet 101 moves in the horizontal direction. Note that the horizontal direction means a direction parallel to the channel bottom (channel bottom wall 212) where the magnetic particles P are captured in the channel 210.

[0107] 14A and 14B, the same components as those in FIGS. 13A and 13B are denoted by the same reference numerals, and the description thereof will be omitted. Also, in FIGS. 14A and 14B, magnetic particles P are assumed to flow within flow channel 210 from the back of the page toward the front of the page. Also, in FIGS. 14A and 14B, flow channel 210 and magnetic flux guide 120 are shown as cross-sectional views.

[0108] 14A and 14B, the magnet 101 is moved laterally (horizontally) by the solenoid unit 113. That is, the magnetic field change unit 110c moves the magnet 101 horizontally. The magnetic flux induction unit 120 is positioned laterally with respect to the particle capture region (displaced from directly below the particle capture region). That is, the magnetic field off position of the magnet 101 is located laterally with respect to the magnetic field on position.

[0109] In the example shown in FIGS. 14A and 14B, the magnet 101 moves laterally (horizontally) by the solenoid part 113, so that the magnet 101 moves between the magnetic field on position and the magnetic field off position.

[0110] The magnetic field change device 100c according to the third embodiment can reduce the space in the vertical direction (the direction perpendicular to the channel bottom (channel bottom wall 212) where the magnetic particles P are captured by the magnetic field generated by the magnet 101) compared to the first and second embodiments. This allows the size of the sample analysis system 1 to be more compact than the first and second embodiments.

[0111] [Fourth embodiment] Next, a fourth embodiment of the present invention will be described with reference to FIGS. 15A and 15B.

[0112] 15A and 15B are diagrams showing the configuration of a magnetic field change device 100 in the fourth embodiment. FIG. 15A shows the case where the magnetic field is on, and FIG. 15B shows the case where the magnetic field is off. In addition, in FIGS. 15A and 15B, the same components as those in FIGS. 4A and 4B are denoted by the same reference numerals, and their description will be omitted. In the example shown in FIGS. 15A and 15B, magnetic particles P are assumed to flow in the horizontal direction of the page (x-axis direction: direction of arrow A1). In addition, in FIGS. 15A and 15B, the flow path 210, magnet 101, and magnetic flux guide 120A are shown as cross-sectional views.

[0113] 15A and 15B, the magnetic field change device 100 is configured such that the magnet 101 is fixed in position, and the magnetic flux induction unit 120A is moved by the magnetic field change unit 110d. Specifically, the magnetic field change unit 110d is configured with an L-shaped arm unit 111 and a solenoid unit 113. The magnetic flux induction unit 120A is provided on one end of the arm unit 111, and the solenoid unit 113 is provided on the other end.

[0114] Then, the arm 111 is moved vertically (perpendicular to the bottom surface of the flow path (flow path bottom wall 212) where the magnetic particles P are captured by the magnetic field generated by the magnet 101) by the solenoid 113, thereby moving the magnetic flux induction unit 120A vertically. That is, in the magnetic field-on state, as shown in FIG. 15A, the magnetic flux induction unit 120A moves downward in the plane of the page (negative side in the z-axis direction: toward the ground), thereby exposing the magnet 101 to the outside of the magnetic flux induction unit 120A. As a result, a magnetic field for capturing the magnetic particles P can be applied to the particle capture region 231.

[0115] As described above, in the fourth embodiment, the magnetic field change unit 110d includes an arm unit 111. The magnetic flux induction unit 120A is provided at one end of the arm unit 111. Furthermore, the solenoid unit 113 that moves the arm unit 111 by a solenoid is provided at the other end of the arm unit 111.

[0116] 15B, magnetic flux induction unit 120A moves upward in the plane of the drawing (positive side in the z-axis direction: opposite to the ground), causing magnet 101 to fit into storage section 121 of magnetic flux induction unit 120A. As a result, most of the magnetic flux MF (see FIGS. 4A and 4B) generated from magnet 101 passes through the interior of magnetic flux induction unit 120A, reducing the strength of the magnetic field outside magnetic flux induction unit 120A. This reduces the effect of the magnetic field on photodetector 201.

[0117] In the first to third embodiments, the magnet 101 moves between a magnetic field-on position and a magnetic field-off position. In contrast, in the fourth embodiment shown in Figures 15A and 15B, the magnetic flux guide 120A moves to switch between the magnetic field-on state and the magnetic field-off state.

[0118] Next, the effects of the fourth embodiment will be described with reference to FIGS. 16A and 16B.

[0119] Fig. 16A is a diagram showing the configuration of a magnetic flux induction unit 120 used in the first to third embodiments. Fig. 16B is a diagram showing the configuration of a magnetic flux induction unit 120A used in the fourth embodiment. Figs. 16A and 16B show the magnetic field-off state and are top perspective views of the magnetic flux induction units 120 and 120A.

[0120] 16A, the magnetic flux guide 120 used in the first to third embodiments is provided with a notch 124 through which the arm 111 (see FIG. 9A, etc.) passes. Therefore, one of the side surfaces of the magnet 101 stored in the storage section 121 is exposed.

[0121] In the fourth embodiment, as shown in FIG. 16B, it is not necessary to provide the magnetic flux induction unit 120A with the notch 124 (see FIG. 16A) for providing the arm 111 (see FIG. 9A, etc.). This makes it possible to cover the surfaces of the magnet 101 other than the upper surface with the magnetic flux induction unit 120A in the magnetic field-off state, as shown in FIG. 16B. As a result, in the magnetic field-off state, it is possible to prevent the magnetic field generated from the magnet 101 from leaking outside the magnetic flux induction unit 120A. Therefore, according to the fourth embodiment, the influence of the magnetic field on the photodetector 201 can be reduced more than in the magnetic field change device 100 of the first to third embodiments.

[0122] (Example of the configuration of the magnetic flux induction unit 120) Next, with reference to FIGS. 17A to 18B, configuration examples of the magnetic flux induction section 120 will be shown.

[0123] 17A to 17C are diagrams showing configuration examples of the magnetic flux induction unit 120 used in the first to third embodiments. In Fig. 17A to Fig. 17C, top views of the magnetic flux induction unit 120 are shown.

[0124] 17A to 17C all have a notch 124 for passing the arm portion 111 (see FIG. 9A, etc.). In the example shown in FIG. 17A, the magnetic flux induction portion 120 has a U-shape, in the example shown in FIG. 17B, the magnetic flux induction portion 120 has a U-shape, and in the example shown in FIG. 17C, the magnetic flux induction portion 120 has a C-shape.

[0125] 18A and 18B are diagrams showing an example of the configuration of a magnetic flux induction unit 120A used in the fourth embodiment, in which top views of the magnetic flux induction unit 120A are shown.

[0126] 18A and 18B do not have notch 124 (see FIGS. 17A to 17C) for passing arm 111 (see FIG. 9A, etc.). Therefore, all surfaces of magnet 101 except for the top surface are covered by magnetic flux induction section 120A. In the example shown in FIG. 18A, the top surface of magnetic flux induction section 120A has a rectangular shape, while in the example shown in FIG. 18B, the top surface of magnetic flux induction section 120A has an oval shape.

[0127] In a first embodiment of this embodiment, the magnet 101 moves up and down as the arm 111 rotates using the rotating part 112. In a second embodiment, the magnet 101 moves up and down using the solenoid part 113. In a third embodiment, the magnet 101 moves horizontally. In a fourth embodiment, the position of the magnet 101 is fixed, and the magnetic flux induction part 120 moves up and down.

[0128] However, the magnetic field change device 100 is not limited to the forms of the first to fourth embodiments, as long as it is possible to place the N pole 101a facing the first surface 122 and the S pole 101b facing the second surface 123.

[0129] For example, the magnetic flux induction unit 120 may move in the horizontal direction, i.e., the magnet 101 and the magnetic flux induction unit 120 may be interchanged in the third embodiment. Alternatively, the magnetic flux induction unit 120 may rotate in the horizontal direction, thereby switching the axis connecting the first surface 122 and the second surface 123 and the magnetization direction A2 between being in different directions and being in the same direction.

[0130] Magnet 101 can move to the outside of magnetic flux guides 120, 120A through cutout 124 or an opening provided in magnetic flux guide 120A, or can be stored in storage section 121 of magnetic flux guides 120, 120A.

[0131] Furthermore, although the magnet 101 is assumed to be a permanent magnet, it may be an electromagnet.

[0132] Note that the above-described configurations, functions, controller 300, storage unit, etc. may be partially or entirely implemented in hardware by, for example, designing them as integrated circuits. Also, as shown in Fig. 3, the above-described configurations, functions, etc. may be implemented in software by a processor such as a CPU interpreting and executing a program that implements each function. Information such as the program, table, and file that implements each function can be stored in a recording device such as memory 301 or an SSD (Solid State Drive), or in a recording medium such as an IC (Integrated Circuit) card, an SD (Secure Digital) card, or a DVD (Digital Versatile Disc).

[0133] In addition, in each embodiment, the control lines and information lines shown are those that are considered necessary for explanation, and not all control lines and information lines in the product are necessarily shown. In reality, it can be considered that almost all components are interconnected. [Explanation of symbols]

[0134] 1. Sample analysis system 100 Magnetic field change device 101 Magnet 101a N pole 101b S pole 110 Magnetic field change section 111 Arm 112 Rotating part 113 Solenoid section 120,120A magnetic flux induction section 121 Storage section 122 First Side 123 Second Side 124 Notch 200 Detector 201 Photodetector (detection unit) 210 Flow path 300 Controller A2 Magnetization direction MF magnetic flux (magnetic field) P magnetic particles (bound to labeling substances)

Claims

1. a magnet that applies a magnetic field to the magnetic particles bound to the labeling substance; a magnetic flux guide portion made of a soft magnetic material; a magnetic field changer that changes the magnetic field around the magnet; Equipped with The magnetic flux guide portion is a first surface facing the north pole of the magnet and a second surface facing the south pole of the magnet; the first surface and the second surface are connected by a member of the magnetic flux guider, The magnetic field change unit is The magnetic field is changed by making the N pole face the first surface and the S pole face the second surface. Magnetic field change device.

2. the magnetic flux induction unit has a storage portion that is a space for storing the magnet, The magnetic field change unit moves the magnet in and out of the storage portion of the magnetic flux guide unit.

2. The magnetic field changing device according to claim 1.

3. The magnetic field change unit moves the magnet in a direction perpendicular to the bottom surface of the flow channel where the magnetic particles are captured by the magnetic field generated by the magnet.

3. The magnetic field changing device according to claim 2.

4. The magnetic field change unit moves the magnet in a direction parallel to the bottom surface of the flow channel where the magnetic particles are captured by the magnetic field generated by the magnet.

3. The magnetic field changing device according to claim 2.

5. The magnetic field change unit moves the magnetic flux induction unit.

3. The magnetic field changing device according to claim 2.

6. The magnetic field change unit includes an arm unit, one end of which is provided with the magnet, and the other end of which is provided with a rotation unit that rotates the arm unit.

2. The magnetic field changing device according to claim 1.

7. The magnetic field change unit has an arm unit, one end of which is provided with the magnet, and the other end of which is provided with a solenoid unit that moves the arm unit by a solenoid.

2. The magnetic field changing device according to claim 1.

8. The magnetic field change unit has an arm unit, one end of the arm unit is provided with the magnetic flux induction unit, and the other end of the arm unit is provided with a solenoid unit that moves the arm unit by a solenoid.

2. The magnetic field changing device according to claim 1.

9. The magnetic flux guide portion is Consisting of iron, silicon steel, permalloy, permendur, magnetic alloy, and soft ferrite 2. The magnetic field changing device according to claim 1.

10. The magnetization direction of the magnet is parallel to the bottom surface of the flow channel where the magnetic particles are captured by the magnetic field generated by the magnet.

2. The magnetic field changing device according to claim 1.

11. The magnet is a permanent magnet.

2. The magnetic field changing device according to claim 1.

12. a magnet that applies a magnetic field to the magnetic particles bound to the labeling substance; a magnetic flux guide portion made of a soft magnetic material; a magnetic field changer that changes the magnetic field around the magnet; and a magnetic field changing device having a detection unit that detects light emitted by the labeling substance; a controller for controlling the magnetic field change unit; A sample analysis system comprising: The magnetic flux guide portion is a first surface facing the north pole of the magnet and a second surface facing the south pole of the magnet; the first surface and the second surface are connected by a member of the magnetic flux guider, The magnetic field change unit, under the control of the controller, changing the magnetic field by causing the north pole to face the first surface and the south pole to face the second surface; The detection unit The light is detected in a state where the N pole faces the first surface and in a state where the S pole faces the second surface. Sample analysis system.

13. a magnet that applies a magnetic field to the magnetic particles bound to the labeling substance; a magnetic flux guide portion made of a soft magnetic material; a magnetic field changer that changes the magnetic field around the magnet; and a magnetic field changing device having a detection unit that detects light emitted by the labeling substance; a controller for controlling the magnetic field change unit; A sample analysis method performed by a sample analysis system comprising: The magnetic flux guide portion is a first surface facing the north pole of the magnet and a second surface facing the south pole of the magnet; the first surface and the second surface are connected by a member of the magnetic flux guider, The magnetic field change unit is controlled by the controller. changing the magnetic field by causing the north pole to face the first surface and the south pole to face the second surface; The sample analysis system comprises: a magnetic particle capturing step of capturing the magnetic particles with the magnetic field generated by the magnet; a detection step in which, after the magnetic particle capturing step, the magnetic field changing unit causes the N pole to face the first surface and the S pole to face the second surface, and the detection unit performs detection; Perform sample analysis method.

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