Antibacterial composition

Abstract

According to an embodiment of the present invention, there is provided an antibacterial composition comprising Lemongrass Oil, Lavender Oil, and Ylang Ylang Oil.

Description

[Technical field]

[0001] The present invention relates to an antibacterial composition. [Background technology]

[0002] Recently, there has been much interest in the development of antibacterial substances derived from natural products. These antibacterial substances are known to have high antibacterial effects due to the combined action of various components derived from natural products, and often have low toxicity to humans or animals.

[0003] In addition, antibacterial substances play an important role in the medical and cosmetic fields, and are used in many cosmetics to solve problems related to skin diseases. For example, many antibacterial cosmetics have been developed to improve skin problems such as hair loss, dandruff, and acne, and such cosmetics can minimize the growth of harmful strains of bacteria present on the skin by using antibacterial substances. Summary of the Invention [Problem to be solved by the invention]

[0004] The present invention has been made to solve the above problems, and an object of the present invention is to provide an antibacterial composition.

[0005] The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the following description. [Means for solving the problem]

[0006] According to an embodiment of the present invention, there is provided an antibacterial composition comprising Lemongrass Oil, Lavender Oil, and Ylang Ylang Oil.

[0007] The weight ratio of the lavender oil and the lemongrass oil may be 1:1.3 to 2.7.

[0008] The weight ratio of the ylang-ylang oil and the lemongrass oil may be 1:4-8.

[0009] The weight ratio of the ylang-ylang oil and the lavender oil may be 1:1.5 to 4.5.

[0010] The weight ratio of the ylang-ylang oil, the lavender oil and the lemongrass oil may be 1:1.5-4.5:4-8.

[0011] In addition, the composition may have antibacterial activity against at least one of Staphylococcus strains, Streptococcus strains, Malassezia strains, Propionibacterium strains, and Candida strains.

[0012] According to an embodiment of the present invention, a cosmetic composition including an antibacterial composition is provided.

[0013] According to an embodiment of the present invention, there is provided an external skin preparation composition comprising an antibacterial composition.

[0014] According to an embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating hair loss, comprising an antibacterial composition. Effect of the Invention

[0015] The antibacterial composition according to the present invention contains lemongrass oil, lavender oil and ylang-ylang oil, and thus can provide excellent antibacterial or antifungal effects against harmful strains of bacteria that cause symptoms such as hair loss, acne or dandruff.

[0016] In addition, the antibacterial composition according to the present invention can alleviate symptoms such as hair loss, acne, or dandruff, and promote hair growth.

[0017] In order to more fully understand the drawings referred to in the detailed description of the invention, a brief description of each of the drawings is provided. [Brief description of the drawings]

[0018] [Figure 1] 1 is a photograph showing the results of a Minimal Inhibitory Concentration (MIC) measurement experiment of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by a skilled artisan in the art to which the present invention belongs. In general, the nomenclature used herein is well known and commonly used in the art. In addition, when describing an embodiment of the present invention, if a specific description of a related known configuration or function is deemed to hinder understanding of the embodiment of the present invention, the detailed description will be omitted. In addition, although an embodiment of the present invention will be described below, the technical idea of ​​the present invention is not limited or restricted thereto, and may be modified and variously implemented by those skilled in the art.

[0020] When any part is referred to herein as including a certain element, this does not mean excluding other elements, but may further include other elements, unless specifically stated to the contrary. As used herein, the term "and / or" includes a combination of multiple associated items or any one item of multiple associated items.

[0021] In the present invention, "oil" refers to a natural vegetable oil extracted from a plant, and is used interchangeably with "essence," "distilled extract," or "essential oil." The plant used to produce the oil may be an original product that has not been subjected to a crushing or grinding process, or may be cut products that have been subjected to a crushing or grinding process, and may include all parts of the plant, such as roots, leaves, stems, flowers, and fruits.

[0022] In the present invention, the oil can be extracted by steam distillation, which is a method of extracting aromatic active ingredients from plants using steam and condensing them by cooling with cooling water. The oil also includes the extract itself and all forms of extract that can be formed using the extract, such as a diluted or concentrated extract obtained by steam distillation of the plant body, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture of these, but is not limited to these.

[0023] In the present invention, the oil may be in a liquid form, a solid form obtained by drying a liquid extract, or a powder form obtained by pulverizing a solid extract. In this case, the drying method may be any of various methods commonly used in the art, such as spray drying, freeze drying, vacuum drying, hot air drying, etc., but is not limited thereto.

[0024] According to an embodiment of the present invention, an antibacterial composition containing Lemongrass Oil, Lavender Oil, and Ylang Ylang Oil is provided. The antibacterial composition according to the embodiment of the present invention uses the above three oils in combination, and as a result of the synergistic effect of each oil, the antibacterial activity can be improved compared to when one or two oils are used.

[0025] Lemongrass oil is an oil extracted from Cymbopogon Schoenanthus and is also known as Cymbopogon Schoenanthus Oil. It is known to have antifungal activity, disinfecting action, deodorizing action and bactericidal action.

[0026] In an embodiment, the antibacterial composition may contain 30-85% by weight of lemongrass oil, specifically 43-75% by weight, more specifically 45-70% by weight, and even more specifically 50-70% by weight, based on the total weight of the antibacterial composition.

[0027] Lavender oil is an oil extracted from lavender (Lavandula angustifolia) and is known to have antibacterial, anti-inflammatory and antioxidant properties, as well as to protect the skin from harmful external substances and to have a soothing and circulatory effect.

[0028] In an embodiment, the antibacterial composition may contain 10-50% by weight, specifically 15-45% by weight, and more specifically 20-40% by weight, of lavender oil based on the total weight of the antibacterial composition.

[0029] Ylang-ylang oil is an oil extracted from the ylang-ylang tree (Cananga odorata) and is known to help balance sebum secretion, relieve skin and scalp symptoms caused by excessive sebum secretion, and strengthen hair.

[0030] In an embodiment, the antibacterial composition may contain ylang-ylang oil in an amount of 1-30% by weight, specifically 3-25% by weight, and more specifically 5-20% by weight, based on the total weight of the antibacterial composition.

[0031] For example, by including lemongrass oil, lavender oil, and / or ylang-ylang oil in the above content range, excellent antibacterial properties, fragrance, usability, etc. can be realized. However, the present invention is not limited to these.

[0032] In an embodiment, the weight ratio of lavender oil and lemongrass oil may be 1:1.3-2.7. Additionally / alternatively, the weight ratio of ylang-ylang oil and lemongrass oil may be 1:4-8. Additionally / alternatively, the weight ratio of ylang-ylang oil and lavender oil may be 1:1.5-4.5. When the weight ratio of lemongrass oil, lavender oil and / or ylang-ylang oil is within the above range, antibacterial activity can be improved. However, the present invention is not limited thereto.

[0033] In the embodiment, the weight ratio of ylang-ylang oil, lavender oil and lemongrass oil may be 1:1.5-4.5:4-8, specifically 1:2-4:4-8, and more specifically 1:2.5-3.5:4-8. When the weight ratio of lemongrass oil, lavender oil and ylang-ylang oil is within the above range, antibacterial activity is maximized, hair loss is minimized, and excellent hair growth is provided. However, the present invention is not limited thereto.

[0034] In an embodiment, the antibacterial composition may have antibacterial activity against harmful strains that cause symptoms such as hair loss, acne, dandruff, etc. For example, the antibacterial composition may have antibacterial activity against at least one of strains of the genus Staphylococcus, strains of the genus Streptococcus, strains of the genus Malassezia, strains of the genus Propionibacterium, and strains of the genus Candida, and more specifically, may have antibacterial activity against strains of the genus Staphylococcus.

[0035] In an embodiment, the antibacterial composition may have antibacterial activity against at least one of Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Malassezia furfur, Propionibacterium acnes, and Candida albicans, specifically, may have antibacterial activity against Staphylococcus aureus.

[0036] According to an embodiment of the present invention, a cosmetic composition is provided. The cosmetic composition may include the antibacterial composition described above.

[0037] In the embodiment, the antibacterial composition has antibacterial activity against harmful strains that cause symptoms such as hair loss, acne, or dandruff, and therefore the cosmetic composition according to the embodiment of the present invention can alleviate symptoms such as hair loss, acne, or dandruff and promote hair growth.

[0038] In the embodiment, the cosmetic composition may further contain additives commonly used in the art within the scope of the present invention. For example, the cosmetic composition may further contain thickeners, pH adjusters, isotonicity agents, surfactants, stabilizers, preservatives, antiseptics, bactericides, moisturizers, blocking agents, antioxidants, organic pigments, inorganic pigments, fragrances, vitamins, etc. The amounts of the above ingredients can be easily selected by those skilled in the art within the scope of the present invention.

[0039] In an embodiment, the cosmetic composition can be provided in a formulation that is commonly produced in the art.For example, the cosmetic composition can be formulated as an oil dispersion formulation, a water dispersion formulation, an oil-in-water (O / W), a water-in-oil (W / O), a water-in-oil-in-water (W / O / W) and an oil-in-water-in-oil (O / W / O) emulsion formulation, a solubilized formulation, etc.However, the cosmetic composition can also be provided in a formulation such as a solution, a suspension, an emulsion, a solid, a gel, an oil, a powder, a paste or a foam aerosol, etc., without being limited thereto.

[0040] In the embodiment, the cosmetic composition may be prepared in various products. For example, it may be prepared in hair washing products such as shampoo, rinse, hair conditioner, hair gel, etc., or in scalp and / or hair care products such as scalp or hair toner, scalp or hair essence, scalp or hair serum, scalp or hair tonic, etc. In addition, it may be prepared in soap, detergent, cleansing cream, cleansing water, etc., or in color cosmetics such as lipstick, lip balm, mascara, blusher, shading, highlighter, makeup base, foundation, fact, concealer, skin cover, etc. In addition, it may be prepared in functional cosmetics such as nutritional cream, essence, ampoule, or basic cosmetics such as toner, lotion, etc. In addition, it may be prepared in products that are attached to the skin such as packs, hydrogel slimming patches, etc., or in products that are sprayed on the skin such as mists, aerosols, etc.

[0041] According to an embodiment of the present invention, a skin topical composition is provided. The skin topical composition may include the antibacterial composition as described above.

[0042] The skin topical composition refers to a preparation that acts on the skin among topical agents, and may include, but is not limited to, pharmaceutical products, quasi-drugs, etc. For example, the skin topical composition may have the dosage form of a cream, gel, skin emulsion, patch, spray, etc.

[0043] In the embodiment, the skin external preparation composition may be appropriately blended with ingredients generally used in skin external preparations such as cosmetics and medicines, such as aqueous ingredients, oily ingredients, powdery ingredients, alcohols, moisturizing agents, thickeners, UV blocking agents, whitening agents, preservatives, antioxidants, surfactants, fragrances, coloring agents, fatty substances, dissolving agents, thickening agents, gelling agents, softening agents, foaming agents, fragrances, sequestering agents, chelating agents, vitamins, various skin nutrients, or combinations thereof, as required. The blending amounts of the above ingredients can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present invention.

[0044] According to an embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating hair loss. The pharmaceutical composition may comprise an antibacterial composition as described above. "Preventing hair loss" means preventing, inhibiting, arresting and reducing partial or complete hair loss.

[0045] "Alopecia" refers to the loss of hair growth and partial or total hair loss, and may include, for example, androgenic alopecia or androgenic pattern alopecia, toxic alopecia, androgenic alopecia, alopecia areata, telogen effluvium, alopecia due to endocrine disorder, metabolic disorder, nutritional disorder, drug-induced alopecia, mechanical alopecia, alopecia associated with skin disease, cicatricial alopecia, congenital alopecia, trichotillomania.For example, alopecia can have many etiologies, including genetic factors, aging, local and systemic diseases, fever symptoms, mental stress, hormonal problems and drug side effects, etc.

[0046] In an embodiment, the pharmaceutical composition can promote hair growth. "Hair growth" can include maintaining, inducing, stimulating, promoting and regenerating hair growth; growing missing hair, extending the anagen phase in the hair cycle, or converting vellus hair to adult hair, etc. Also, "promoting hair growth" means promoting hair growth, extending the anagen phase in the hair cycle, or converting vellus hair, which is thin, smooth, short and bleached hair whose root is located on the surface of the dermis, to adult hair, which is thick, long and pigmented hair whose root is deep in the dermis.

[0047] In an embodiment, the pharmaceutical composition may further contain a pharma- ceutically acceptable salt within a range that does not impair the effects of the present invention. Here, the "pharma- ceutically acceptable salt" may be a salt that does not induce severe irritation to an organism to which the compound is administered and does not impair the biological activity and physical properties of the compound.

[0048] For example, pharma- ceutically acceptable salts can be formed from inorganic acids, such as hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, or organic acids, including acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, N-acetylcysteine, and the like.

[0049] Also, for example, pharma- ceutically acceptable salts can be prepared by adding an inorganic or organic base to the free acid. Salts derived from inorganic bases may include, but are not limited to, sodium, potassium, lithium, ammonium, calcium, magnesium salts, etc. Salts derived from organic bases include, but are not limited to, salts of primary, secondary and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, ethanolamine, lysine, arginine, N-ethylpiperidine, piperidine, polyimine resins, and the like.

[0050] In the embodiment, the pharmaceutical composition may further contain a pharma- ceutically acceptable carrier or additive within a range that does not impair the effects of the present invention. Here, the "pharma- ceutically acceptable carrier" may be a compound that is commonly used in formulation, does not suppress the activity of an active ingredient, does not have toxicity beyond the range that is applicable to the subject of application or formulation, and facilitates the addition of a compound into cells or tissues.

[0051] In an embodiment, the pharmaceutical composition may be formulated as a solid formulation or a liquid formulation. For example, when formulated as a solid formulation, it may further include a carrier, a flavoring agent, a binder, a preservative, a disintegrant, a lubricant, a filler, etc., and when formulated as a liquid formulation, it may further include a solution such as water or a propylene glycol solution, a suspension, an emulsion, etc., but is not limited thereto, and it may further include a coloring agent, a flavoring agent, a stabilizer, a viscosity agent, etc.

[0052] In the embodiment, the solid preparation may include powder, granule, tablet, capsule, suppository, etc. For example, the powder can be prepared by simply mixing the mekabu extract, which is the active ingredient of the present invention, with a carrier such as lactose, starch, microcrystalline cellulose, etc. The granule can be prepared by mixing the mekabu extract of the present invention with a carrier, a binder such as polyvinylpyrrolidone, hydroxypropylcellulose, etc., and then using a wet granulation method using a solvent such as water, ethanol, isopropanol, etc., or a dry granulation method using compression force. In addition, the tablet can be prepared by mixing the granule with a lubricant such as magnesium stearate, and then tableting using a tablet press.

[0053] In embodiments, the pharmaceutical composition may be administered orally, by injection (e.g., intramuscular injection, intraperitoneal injection, intravenous injection, infusion, subcutaneous injection, implant), inhalation, nasal administration, vaginal administration, rectal administration, sublingual administration, transdermal administration, topical administration, etc., depending on the disease or individual condition to be treated, but is not limited thereto. In addition, the pharmaceutical composition of the present invention may be formulated into an appropriate dosage unit form containing a non-toxic, pharma- ceutical acceptable carrier, additive, or vehicle that is commonly used depending on the administration route.

[0054] In the embodiment, the dosage of the pharmaceutical composition varies depending on the patient's condition and body weight, the degree of the disease, the drug form, the administration route and period, but can be appropriately selected by those skilled in the art. The administration form, administration route and administration method of the pharmaceutical composition may be independent of each other and are not particularly limited in the method, and can follow any administration route and administration method as long as the pharmaceutical composition can reach the target site. For example, the administration form of the pharmaceutical composition according to the present invention can be used in the form of these pharma-ceutically acceptable salts, and can be used alone or in combination with other pharma-ceutical active compounds, as well as in an appropriate combination. For example, the administration method of the pharmaceutical composition according to the present invention can be intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, as well as a method of applying the composition to the disease site, spraying, or inhaling the composition, but is not limited thereto.

[0055] [Experimental Example] The following examples are provided to more specifically explain the present invention, but the present invention is not limited to these.

[0056] Experimental example 1: Confirmation of synergistic effects by using mixed oils (1) Preparation of Examples 1 to 3 Examples of compositions according to the present invention were prepared using the components shown in Table 1 below. In Table 1, the unit of component content is % by weight.

[0057] [Table 1]

[0058] The specific manufacturing method is as follows. After mixing the oil components, the mixture was stirred uniformly for 10 minutes to complete the antibacterial compositions of Examples 1 to 3.

[0059] (2) Preparation of Comparative Examples 1 to 6 Comparative examples of compositions according to the present invention were prepared using the components shown in Table 2 below. In Table 2, the unit of component content is % by weight.

[0060] [Table 2]

[0061] The specific manufacturing method is the same as in Examples 1-3.

[0062] (3) Measurement of Minimal Inhibitory Concentration (MIC) The antibacterial activity of Example 1 and Comparative Example 1 was evaluated using Minimal Inhibitory Concentration (MIC), and the results are shown in Table 3 and FIG.

[0063] The experimental method is as follows. 100 μl of 200% TSB was added to a 96-well plate, and a sample was prepared so that the oil concentration was twice that of the oil to be treated, and 100 μl of the sample was dispensed into the 96-well plate. Then, the samples of Example 1 and Comparative Example 1 were adjusted to 4000 ppm, 800 ppm, 160 ppm, and 32 ppm, respectively, and then cultured at a temperature of 32.5° C. for 24 hours. After a certain period of time, the presence or absence of bacterial growth was determined by a color change (yellow indicates bacterial growth; colorless indicates bacterial growth inhibition). Control group 1 (C1) is a medium without inoculation of the bacterial strain, control group 2 (C2) is a medium without the addition of the antibacterial composition after inoculation of the bacterial strain, the positive control group (C3) is a medium with phenoxyethanol added instead of the antibacterial composition, and the negative control group (C4) is a medium with DMSO added.

[0064] [Table 3]

[0065] As a result of the experiment, in the case of Example 1 in which three types of oils were mixed and used, the color appeared yellow at 800 ppm, whereas the color appeared colorless at 4000 ppm. Therefore, it was found that the minimum inhibitory concentration of Example 1 against Staphylococcus aureus was 800 to 4000 ppm.

[0066] In addition, in the case of Comparative Example 1 containing only lemongrass oil, the oil appeared yellow even at 4000 ppm, indicating that the minimum inhibitory concentration of Comparative Example 1 against Staphylococcus aureus exceeded 4000 ppm.

[0067] That is, it was confirmed that Example 1 had improved antibacterial activity against Staphylococcus aureus compared to Comparative Example 1.

[0068] (4) Paper Disk Diffusion Assay The antibacterial activity of the above-mentioned Examples 1 to 3 and Comparative Examples 2 to 6 was evaluated using a Paper Disk Diffusion Assay, and the results are shown in Table 4.

[0069] The experimental method is as follows. Staphylococcus aureus strain was inoculated onto tryptic soy agar using a 100% loop and cultured for 24 hours. After that, the cultured strain was added to 0.85% NaCl at 1.0 × 10 7 The bacteria were suspended in a solution so that the bacteria concentration was 1.0×10. The bacteria solution was then dispensed in an amount of 100 μl into the TSA medium, which was then smeared with glass beads to obtain a bacterial count of 1.0×10. 6 The samples of the Examples and Comparative Examples were each dispensed onto a paper disk at 40 μl / disk, and after being fully absorbed into the paper disk, the paper disk was placed on the medium on which the bacterial strain was smeared, and the sample-treated plate was turned upside down and cultured at 32.5° C. for 24 hours. After the culture, the short and long diameters of the inhibition zone around the disk were measured, and the average value was calculated to evaluate the antibacterial activity.

[0070] [Table 4]

[0071] As a result of the experiment, in the case of Examples 1 to 3 in which lemongrass oil, lavender oil and ylang-ylang oil were used in combination, the diameter of the growth inhibition zone was measured to be 43 mm or more in all cases, demonstrating excellent antibacterial activity.

[0072] In addition, in the case of Comparative Examples 2 and 3, which contained only one of lemongrass oil, lavender oil, and ylang-ylang oil, the diameter of the growth inhibition zone was measured to be less than 26 mm, and in the case of Comparative Examples 4 to 6, which contained only two of the above oils, the diameter of the growth inhibition zone was measured to be less than 38 mm in all cases, indicating that the antibacterial activity was lower than in Examples 1 to 3.

[0073] Experimental Example 2: Confirmation of the effect of oil weight ratio (1) Preparation of Comparative Examples 7 to 13 Examples of compositions according to the present invention were prepared using the components listed in Table 5 below. In Table 5, the unit of component content is % by weight.

[0074] [Table 5]

[0075] The specific manufacturing method is the same as in Examples 1-3.

[0076] (2) Paper Disk Diffusion Assay The antibacterial activity of the above-mentioned Examples 1 to 3 and Comparative Examples 7 to 13 was evaluated using the Paper Disk Diffusion Assay in the same manner as in Experimental Example 1, and the results are shown in Table 6.

[0077] [Table 6]

[0078] As a result of the experiment, in the case of Examples 1 to 3, which contained lemongrass oil, lavender oil and ylang-ylang oil in weight ratios within the range of the examples, the diameter of the growth inhibition zone was measured to be 43 mm or more in all cases, indicating that the antibacterial activity was excellent.

[0079] In addition, in the case of Comparative Examples 7 to 13, which contained lemongrass oil, lavender oil, and ylang-ylang oil in weight ratios outside the range of the Examples, the diameter of the growth inhibition zone was measured to be less than 37 mm in all cases, indicating that the antibacterial activity was lower than that of Examples 1 to 3.

[0080] Although the specific parts of the present invention have been described in detail above, those skilled in the art will recognize that these specific techniques are merely preferred embodiments and do not limit the scope of the present invention. Therefore, the true scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. Contains Lemongrass Oil, Lavender Oil and Ylang Ylang Oil An antibacterial composition comprising:

2. The weight ratio of the lavender oil and the lemongrass oil is 1:1.3-2.

7. The antibacterial composition according to claim 1.

3. The weight ratio of the ylang-ylang oil and the lemongrass oil is 1:4-8. The antibacterial composition according to claim 1.

4. The weight ratio of the ylang-ylang oil and the lavender oil is 1:1.5-4.

5. The antibacterial composition according to claim 1.

5. The weight ratio of the ylang-ylang oil, the lavender oil and the lemongrass oil is 1:1.5-4.5:4-8. The antibacterial composition according to claim 1.

6. The composition has antibacterial activity against at least one of Staphylococcus, Streptococcus, Malassezia, Propionibacterium, and Candida. The antibacterial composition according to claim 1.

7. The antibacterial composition according to any one of claims 1 to 6 is included. A cosmetic composition comprising:

8. The antibacterial composition according to any one of claims 1 to 6 is included. A skin topical composition comprising:

9. The antibacterial composition according to any one of claims 1 to 6 is included. A pharmaceutical composition for preventing or treating hair loss, comprising:

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