Centrifugal cup, cell centrifugation method and centrifugation equipment

Abstract

The present invention discloses a centrifuge cup, which relates to the concentration of cell fluid in the field of cell processing. The centrifuge cup comprises a bowl (1), a cup cap (2), a first region partition plate (3), a second region partition plate (4), and a third region partition plate (5). There is a predetermined gap (7) between one end of the first region partition plate (3) facing the side wall of the bowl (1) and the inner wall of the bowl (1). Drains are respectively formed between the outer end of the second region partition plate (4) and the inner wall of the bowl (1), and between the outer end of the third region partition plate (5) and the inner wall of the bowl (1). An inlet flow path (10) and an outlet flow path (11) are formed in the cup cap (2) and / or the bowl (1). The inlet flow port (14) of the inlet flow path (10) is located within the predetermined gap (7), and the distance between the inlet flow path (10) and the inner wall of the bowl is within a first predetermined range. The distance between the outlet flow port (15) of the outlet flow path (11) and the inner wall of the bowl is within a second predetermined range, and the outlet flow path (11) is located between the second region partition plate (4) and the third region partition plate (5). The present invention further discloses a cell centrifugation method and a centrifuge equipment. The centrifuge cup realizes high-speed rotation, reduction of leakage in the case of high flow rate, rapid concentration on the basis of continuous flow concentration, and improves the yield and activity rate.

Description

Technical Field

[0001] The present invention relates to the technical field of cell processing, and particularly to a centrifuge cup, a cell centrifugation method, and a centrifugation facility.

Background Art

[0002] In the field of cell processing, for subsequent cell processing such as storage and transportation, it is necessary to concentrate the cell solution, that is, to concentrate a low-concentration cell solution into a high-concentration cell solution. Therefore, it is common to concentrate cells by the centrifugal sedimentation method. Specifically, the cell solution is placed in a centrifuge cup, and the cells are extruded to the edge of the centrifuge cup by centrifugal force at high speed rotation, and the supernatant close to the rotation center in the centrifuge cup is extracted to complete the concentration of the cell solution. In the concentration of cells based on the centrifugal sedimentation method, paying attention to two characteristic indexes of the yield and the activity rate, the yield is the ratio of the cells escaped from the outlet of the centrifuge cup during concentration, that is, yield = the number of viable cells after treatment / the number of viable cells before treatment, and the activity rate is the ratio of the viable cells that can withstand the fluid shear by centrifugation, that is, activity rate = the number of viable cells / the total number of cells.

[0003] Currently, many centrifuge cups cause damage to cells due to large shear when the rotation speed is 3000 rpm or more, resulting in a low yield. However, when the rotation speed is 2500 rpm or less, a high flow rate cannot be used, the rotation speed becomes low, and the sedimentation speed of the cells also decreases accordingly, resulting in a low activity rate. Therefore, in the conventional continuous flow concentration technology, in the case of a centrifuge cup with a high flow rate and a high rotation speed, the yield and the activity rate are low, and the concentration effect is insufficient.

Summary of the Invention

Problems to be Solved by the Invention

[0004] The present invention provides a centrifuge cup, a cell centrifugation method, and a centrifugation facility. The centrifuge cup optimizes the flow field and changes the cell trajectory during continuous flow concentration, thereby realizing a reduction in leakage and rapid concentration in the case of high-speed rotation and high flow rate, and also increasing the yield and the activity rate.

Means for Solving the Problem

[0005] To achieve the above object, the present invention provides the following technical forms. The present application provides a centrifuge cup, which includes a bowl, a cup cap, a first region partition plate, a second region partition plate, and a third region partition plate. The extending direction of the first region partition plate is from the center of the bowl towards the side wall of the bowl, and there is a predetermined gap between one end towards the side wall of the bowl and the inner wall of the bowl. Drains are respectively formed between the outer end of the second region partition plate and the inner wall of the bowl, and between the outer end of the third region partition plate and the inner wall of the bowl. An inlet flow path and an outlet flow path are installed in the cup cap and / or the bowl. The inflow port of the inlet flow path is located within the predetermined gap. The distance between the inlet flow path and the inner wall of the bowl is within a first predetermined range. The distance between the outflow port of the outlet flow path and the inner wall of the bowl is within a second predetermined range. And the outlet flow path is located between the second region partition plate and the third region partition plate.

[0006] Furthermore, the existence of a predetermined gap between one end towards the side wall of the bowl and the inner wall of the bowl as described above means that An end plate is installed at one end towards the side wall of the bowl, and a gap is formed between the end plate and the inner wall of the bowl.

[0007] Furthermore, the end plate is arc-shaped, and the arc length is 5 mm or more.

[0008] Furthermore, the first predetermined range is 0.1 mm to 20 mm.

[0009] Furthermore, the second predetermined range is 4 mm or more.

[0010] Furthermore, the first region partition plate, the second region partition plate, and the third region partition plate are installed with gaps in order along the rotation direction of the bowl. The first region partition plate, the second region partition plate, and the third region partition plate each extend along the radial direction of the bowl and are distributed in a Y shape. The first region partition plate is fixedly connected to the cup cap or the bowl where the inlet flow path is installed. The second region partition plate and the third region partition plate are fixedly connected to the cup cap or the bowl.

[0011] Furthermore, the drain formed between the outer end of the second region partition plate and the inner wall of the bowl, and the drain formed between the outer end of the third region partition plate and the inner wall of the bowl are each smaller than the distance between the inlet flow port and the inner wall of the bowl.

[0012] Furthermore, the second region partition plate and the third region partition plate are distributed on both sides of the outlet flow port.

[0013] Furthermore, the present application provides a cell centrifugation method. The cell centrifugation method performs a cell centrifugation operation using the centrifugation cup according to any one of the above items. The cell centrifugation method includes a step of flowing a liquid to be treated in from the inlet flow port, a step of driving the centrifugation cup connected to the rotating device to rotate by the rotating device, thereby circulating and centrifuging the liquid to be treated from the periphery of a predetermined gap to the drain, and a step of extracting, from the outlet flow port, an outflow liquid that is the liquid present in the vicinity of the outlet flow port after the liquid to be treated is centrifuged during centrifugation.

[0014] The present application provides a centrifugation facility including the centrifugation cup according to any one of the above items.

Advantages of the Invention

[0015] Compared with the prior art, the present invention has the following beneficial effects. 1. In the centrifuge cup of the present invention, a first region partition plate, a second region partition plate, and a third region partition plate are installed. The three region partition plates realize the control of the flow of cell fluid. A predetermined gap is formed between one end of the first region partition plate facing the side wall of the bowl and the inner wall of the bowl. By installing an inflow port for transporting cell fluid into the bowl within the predetermined gap, the position of the inflow port for transporting cell fluid into the bowl is away from the rotation center, providing a large centrifugal force for the sedimentation of cells. Also, according to the flow pattern in which the cell fluid enters the predetermined gap from the inflow port and the flow direction of the liquid is converted to the tangential direction, the influence of fluid perturbation is smaller than that of centrifugal sedimentation, and the cells will gradually adhere to the wall. As the liquid with cells continues to flow along the circumferential wall surface, the fluid perturbation is further reduced, and the cells are sufficiently sedimented until they adhere to the wall. Through the structural design of the predetermined gap and the inflow port, the control of the supply speed and acceleration is possible, the trajectory of the cells is controlled, the rapid sedimentation of the cells is realized, and the centrifugation yield and activity rate are increased.

[0016] 2. In the centrifuge cup of the present invention, three region partition plates distributed in a Y shape are installed. A cell fluid inlet region is formed between the arc-shaped end plate and the inner wall of the bowl. The inlet region is for converting the flow direction of the inflowing liquid from the radial direction to the tangential direction. A development region is formed between the first region partition plate and the second region partition plate. The development region is for continuously flowing the cell fluid along the circumferential wall surface together with the liquid with cells, further reducing the fluid perturbation, and sufficiently sedimenting the cells until they adhere to the wall. An outlet region is formed between the second region partition plate and the third region partition plate. The outlet region is for completing the separation of the cells and the supernatant by forming a huge vortex and increasing the centrifugation yield and activity rate.

[0017] 3. The centrifuge cup of the present invention can form drains between the installed second region partition plate and the third region partition plate, between the second region partition plate and the inner wall of the bowl, and between the third region partition plate and the inner wall of the bowl, respectively. The second region partition plate and the third region partition plate can provide a flow field situation similar to a predetermined gap. The gap formed by the drain induces the cells to move along the circumferential wall surface, sediment them to be closely attached to the circumferential wall surface forcibly, further avoid cell perturbation, and continuously sediment the cells while remaining adhered to the wall. Also, since the second region partition plate and the third region partition plate are close to the outflow port, when the cells adhere to the wall, the liquid will flow out from the outflow port, avoiding the cells from rising near the outflow port, reducing cell loss, and increasing the centrifugation yield and activity rate.

[0018] 4. By installing the outflow port between the second region partition plate and the third region partition plate, the present invention can form a huge vortex between the second region partition plate and the third region partition plate, enabling the separation of cells and the supernatant to be carried out gently and clearly, and increasing the centrifugation yield and activity rate.

[0019] Therefore, according to the centrifuge cup with the above configuration, for both the velocity and acceleration of the liquid, cell movement can be optimized, reducing cell leakage in the case of low rotation speed and high flow rate, realizing rapid concentration of the cell solution, and increasing the yield and activity rate.

Brief Description of the Drawings

[0020]

Figure 1

Figure 2

Figure 3

Figure 4

Embodiments for Carrying Out the Invention

[0021] Hereinafter, with reference to the drawings in the embodiments of the present invention, the technical aspects in the embodiments of the present invention will be clearly and completely described. Of course, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without inventive efforts also belong to the protection scope of the present invention.

[0022] Example 1 This example provides a centrifuge cup. As shown in the configurations of FIGS. 1, 2, and 3, the centrifuge cup includes a bowl 1, a cup cap 2, a first region partition plate 3, a second region partition plate 4, and a third region partition plate 5. A circular cavity for accommodating cell fluid is formed in the bowl 1. The cup cap 2 is installed to airtightly cover the top of the bowl 1. The radius and height of the bowl 1 can be set as required. The radius of the bowl 1 may be 30 mm to 80 mm, and the height of the bowl 1 may also be 30 mm to 80 mm. Preferably, the radius of the bowl 1 is 30 mm, and the height of the bowl 1 is preferably 35 mm.

[0023] The extending direction of the first region partition plate 3 is from the center of the bowl 1 towards the side wall direction of the bowl 1, and there is a predetermined gap between one end towards the side wall of the bowl 1 and the inner wall of the bowl 1. Drains are respectively formed between the outer end of the second region partition plate 4 (i.e., the end close to the inner wall of the bowl 1) and the inner wall of the bowl 1, and between the outer end of the third region partition plate 5 (i.e., the end close to the inner wall of the bowl 1) and the inner wall of the bowl 1.

[0024] In the cup cap 2 and / or the bowl 1, an inlet flow path 10 and an outlet flow path 11 are provided. The inflow port 14 of the inlet flow path 10 is located within a predetermined gap 7, and the distance between the inlet flow path 10 and the inner wall of the bowl 1 is within a first predetermined range. The distance between the outflow port 15 of the outlet flow path 11 and the inner wall of the bowl 1 is within a second predetermined range, and the outlet flow path 11 is located between the second region partition plate 4 and the third region partition plate 5. Among them, the first predetermined range and the second predetermined range may be set as required. For example, the first predetermined range is 0.01 to 30 mm, and the second predetermined range is 4 mm to 20 mm.

[0025] In one embodiment, the first predetermined range is 0.1 mm to 20 mm.

[0026] In one embodiment, the second predetermined range is 4 mm or more.

[0027] In one embodiment, when it is necessary to concentrate cell fluid by a centrifuge cup, the bowl 1 rotates clockwise. As shown in FIG. 3, the first region partition plate 3, the second region partition plate 4, and the third region partition plate 5 are arranged in sequence with gaps along the rotation direction of the bowl 1. However, the first region partition plate 3, the second region partition plate 4, and the third region partition plate 5 may also be arranged in sequence with gaps along the direction opposite to the rotation direction of the bowl 1. The first region partition plate 3, the second region partition plate 4, and the third region partition plate 5 each extend along the radial direction of the bowl 1 and are distributed in a Y shape. The first region partition plate 3 is fixedly connected to the cup cap 2 or the bowl 1 where the inlet flow path 10 is installed. The second region partition plate 4 and the third region partition plate 5 are fixedly connected to the cup cap 2 or the bowl 1. There are gaps between the second region partition plate 4 and the first region partition plate 3, between the second region partition plate 4 and the third region partition plate 5, and between the third region partition plate 5 and the first region partition plate 3, respectively. The angle between the second region partition plate 4 and the third region partition plate 5 is 10° to 120°, for example, 10°, 20°, 30°, 40°, 50°, 60°, 80°, 90°, 100°, 120°. As shown in the configuration of FIG. 3, preferably, the angle between the second region partition plate 4 and the third region partition plate 5 is 60°.

[0028] The first region partition plate 3 extends from the center of the bowl 1 in the direction of the side wall of the bowl 1, and there is a predetermined gap 7 between one end facing the side wall of the bowl 1 and the inner wall of the bowl 1. In one embodiment, an end plate 6 is installed at one end facing the side wall of the bowl 1, and a gap for forming the predetermined gap 7 is formed between the end plate 6 and the inner wall of the bowl 1. The end plate 6 is useful for reducing damage to cells when liquid flows in from the inflow port 14. In one embodiment, an end plate 6 is installed at one end facing the side wall of the bowl 1. In one embodiment, the curvature of the end plate 6 may be the same as the curvature of the inner side wall of the bowl 1, so that an arc-shaped gap is formed between the end plate 6 and the inner wall of the bowl 1. One end of the first region partition plate 3 is installed near the center of the bowl 1 and overlaps the rotation axis of the bowl 1, and the other end is installed near the inner side wall of the bowl 1, and the end plate 6 is fixedly connected. The arc length of the end plate 6 is 5 mm or more. A first drain 8 is formed between the outer end of the second region partition plate 4 and the inner wall of the bowl 1, and a second drain 9 is formed between the outer end of the third region partition plate 5 and the inner wall of the bowl 1. The distance between the end plate 6 and the side wall of the bowl 1, the distance between the second region partition plate 4 and the side wall of the bowl 1, and the distance between the third region partition plate 5 and the side wall of the bowl 1 are each 0.1 mm to 10 mm. The distance between the second region partition plate 4 and the inner wall of the bowl 1 may be 3 mm, the distance between the third region partition plate 5 and the inner wall of the bowl 1 may be 3 mm, and the distance between the inflow port 14 and the side wall of the bowl 1 is smaller than the distance between the end plate 6 and the side wall of the bowl 1.

[0029] Among them, the shape of the end plate 6 can be set as needed. For example, the shape of the end plate 6 is an arc shape, a T shape, a triangular shape, etc.

[0030] In one embodiment, the first drain 8 formed between the outer end of the second region partition plate 4 and the inner wall of the bowl 1, and the second drain 9 formed between the outer end of the third region partition plate 5 and the inner wall of the bowl 1 are each smaller than the distance between the inflow port 14 and the inner wall of the bowl 1. The cup cap 2 and / or the bowl 1 are provided with an inlet channel 10 and an outlet channel 11. That is, the inlet channel 10 may be provided in the cup cap 2 or the bowl 1, or may be provided in both the cup cap 2 and the bowl 1. The same applies to the outlet channel 11. As shown in the configurations of FIGS. 2 and 3, the inlet channel 10 first extends along the center of the cup cap 2 and the bowl 1 to the inner bottom surface of the bowl 1, and then extends along the bottom edge of the first region partition plate 3 to the predetermined gap 7 to communicate the inlet 12 and the inflow port 14. The outlet channel 11 extends along the radial direction of the rotation center and the bottom surface of the cup cap 2 to communicate the outlet 13 and the outflow port 15. The inlet 12 of the inlet channel 10 and the outlet 13 of the outlet channel 11 may be located at the top of the cup cap 2, respectively. The inflow port 14 of the inlet channel 10 is located within the predetermined gap 7, and the distance between the inflow port 14 and the inner wall of the bowl 1 is 5 mm. The outflow port 15 of the outlet channel 11 is installed at the bottom of the cup cap 2, and the distance between it and the inner wall of the bowl 1 is 20 mm to 30 mm, and preferably 25 mm. The outflow port 15 is located between the second region partition plate 4 and the third region partition plate 5. The second region partition plate 4 and the third region partition plate 5 are symmetrically distributed on both sides of the outflow port 15, but the second region partition plate 4 and the third region partition plate 5 may be generally symmetrically distributed on both sides of the outflow port 15. The areas of the inflow port 14 and the outflow port 15 are each less than 9 mm 2 and preferably 4 mm 2 and the inflow port 14 and the outflow port 15 are each square.

[0031] In the above-mentioned centrifuge cup, a first region partition plate 3, a second region partition plate 4, and a third region partition plate 5 are installed. By means of the three region partition plates, control over the flow of cell fluid is realized. At one end of the first region partition plate 3 facing the side wall of the bowl 1, an arc-shaped end plate 6 is installed. An arc-shaped gap is formed between the end plate 6 and the inner wall of the bowl 1. By installing an inflow port 14 for transporting cell fluid into the bowl 1 within the arc-shaped gap, an inlet region of cell fluid is formed within the arc-shaped gap, and the position of the inflow port 14 for transporting cell fluid into the bowl 1 is away from the rotation center, providing a large centrifugal force for the sedimentation of cells. Also, when the cell fluid enters the arc-shaped gap from the inflow port 14 and is constrained by the gap wall surface, the flow direction of the liquid is converted to the tangential direction, and the influence of fluid perturbation is smaller than the influence of centrifugal sedimentation. Therefore, the cells gradually adhere to the wall. As the liquid with cells continues to flow along the circumferential wall surface, the fluid perturbation is further reduced, and the cells are sufficiently sedimented until they adhere to the wall. Through the structural design of the arc-shaped gap and the inflow port 14, the supply speed and acceleration can be controlled, enabling the control of cell trajectories and realizing the rapid sedimentation of cells. The end plate 6 installed at the end of the first region partition plate 3 allows the cell fluid flowing into the bowl 1 to enter the arc-shaped gap from the inflow port 14, and the cell fluid forms a circumferential flow. At this time, since the cell velocity is along the circumferential direction, the control of the initial movement characteristics and initial trajectories of the cells is simplified, and the appropriate control of the initial trajectories of the cells is the key to the continuous sedimentation of the cells.

[0032] The centrifuge cup can form a first drain 8 between the second region partition plate 4 and the inner wall of the bowl 1, and a second drain 9 between the third region partition plate 5 and the inner wall of the bowl 1 by the installed second region partition plate 4 and third region partition plate 5. The centrifuge cup can provide a flow field situation similar to an arc-shaped gap between the bowl 1 by the installed second region partition plate 4 and third region partition plate 5. The gap formed by the drain guides the cells to move along the circumferential wall surface, and sediment until the cells are forced to adhere closely to the circumferential wall surface, further avoiding cell perturbation and continuously sedimenting the cells while adhering to the wall. When the distance between the outflow port 15 and the inner wall of the bowl 1 is within a second predetermined range, preferably, the second predetermined range is 20 mm to 30 mm. Thereby, when the centrifuge cup rotates, the liquid is formed into an annular liquid surface by centrifugal force. When the liquid is higher than the outflow port 15, it is quickly extracted, so that the liquid surface becomes stable and the control is simplified. By ensuring the coincidence of the fluid trajectory, the control effect of the liquid flow of the first drain 8 is further ensured. In addition, since the second region partition plate 4 and the third region partition plate 5 are close to the outflow port 15, when the cells adhere to the wall, the liquid will flow out from the outflow port 15, avoiding the cells from rising near the outflow port 15 and reducing cell loss.

[0033] The centrifuge cup has an outflow port 15 installed at the bottom of the cup cap 2, positions the outflow port 15 at the top of the bowl 1, and by the installed outflow port 15, the liquid reaching the outflow port 15 can be made to flow out of the centrifuge cup, thereby determining the liquid level position in the centrifuge cup and maintaining a stable liquid level in the centrifuge cup.

[0034] The centrifuge cup has three region partition plates distributed in a Y shape installed in the cup cap 2 or the bowl 1. An inlet region for cell fluid is formed between the arc-shaped end plate 6 and the inner wall of the bowl 1, and the inlet region is for converting the flow direction of the inflowing liquid from the radial direction to the tangential direction. A development region is formed between the first region partition plate 3 and the second region partition plate 4, and the development region is for continuously flowing the cell fluid along the circumferential wall surface together with the liquid with cells, further reducing fluid perturbation, and allowing the cells to sediment sufficiently until they adhere to the wall. Due to the development region, it is possible to continuously enhance the effect of cell sedimentation according to the flow law in the arc-shaped gap. The outflow port 15 is installed between the second region partition plate 4 and the third region partition plate 5, and an outlet region for forming a huge vortex between the second region partition plate 4 and the third region partition plate 5 is formed between the second region partition plate 4 and the third region partition plate 5, thereby completing the separation of cells and the supernatant, and enhancing the centrifugation yield and activity rate.

[0035] Therefore, the centrifuge cup with the above configuration can optimize cell movement in terms of both the velocity and acceleration of the liquid, achieve a reduction in cell leakage in the case of high-speed rotation and high flow rate, realize rapid concentration of the cell fluid, and also enhance the yield and activity rate.

[0036] Furthermore, there are radial gaps between the second region partition plate 4 and the first region partition plate 3, and between the third region partition plate 5 and the first region partition plate 3, respectively.

[0037] Verification was carried out with T cells. After multiple concentration tests, the yield and activity rate after human T cell concentration using the above centrifuge cup are shown in Table 1 below.

[0038] JPEG2025518270000002.jpg255161

[0039] With the above-mentioned centrifuge cup, continuous flow concentration of cell fluid can be achieved. A continuous flow is formed between the inlet and the outlet, and the flow rate of the cell fluid can reach 100 ml / min to 250 ml / min. Moreover, the rotational speed of the centrifuge cup during concentration may be 2000 rpm to 2500 rpm.

[0040] When the outflow port 15 is installed at the bottom of the cup cap 2 of the centrifuge cup, the outflow port 15 is located at the top of the bowl 1. By allowing the liquid arriving at the installed outflow port 15 to flow out of the centrifuge cup, the liquid level position in the centrifuge cup can be determined, and a stable liquid level in the centrifuge cup can be maintained.

[0041] Example 2 This example provides a cell centrifugation method. As shown in FIG. 4, the cell centrifugation method performs cell centrifugation operation using the centrifuge cup of the above example. The cell centrifugation method includes: Step S10 of introducing the liquid to be treated from the inflow port 14; Step S20 of driving the centrifuge cup connected to the rotating device to rotate by the rotating device, so as to cause the liquid to be treated to circulate from the periphery of the predetermined gap 7 to the drain for centrifugation; During centrifugation, step S30 of extracting the outflow liquid, which is the liquid existing near the outflow port 15 after the liquid to be treated is centrifuged, by the outflow port 15.

[0042] The liquid to be processed is a solution that requires centrifugation of cells. The liquid to be processed flows in from the inflow port 14, and a cell liquid inlet region is formed between the arc-shaped end plate 6 and the inner wall of the bowl 1. The inlet region is for converting the flow direction of the inflowing liquid from the radial direction to the tangential direction. A development region is formed between the first region partition plate 3 and the second region partition plate 4. The development region is for continuously flowing the cell liquid along the circumferential wall surface together with the liquid with cells. The fluid perturbation is further reduced, and the cells are sufficiently sedimented until they adhere to the wall. In the rotation of the centrifuge cup, due to the development region, it is possible to continuously enhance the effect of cell sedimentation according to the flow law in the predetermined gap 7. By installing the outflow port 15 between the second region partition plate 4 and the third region partition plate 5, an outlet region for forming a huge vortex between the second region partition plate 4 and the third region partition plate 5 is formed between the second region partition plate 4 and the third region partition plate 5. Therefore, the present invention realizes flowing in the liquid to be processed from the inflow port 14, and by driving the centrifuge cup connected to the rotating device to rotate by the rotating device, the liquid to be processed is circulated and centrifuged from the periphery of the predetermined gap 7 to the drain, and during centrifugation, the outflow liquid is extracted through the outflow port 15. Thereby, the separation of the cells and the supernatant is gently and clearly performed, the outflow liquid, which is the liquid present near the outflow port 15 after the liquid to be processed is centrifuged, can be extracted, and the yield and activity rate of the outflow liquid obtained by centrifugation are increased.

[0043] Example 3 This example provides a centrifugation facility equipped with the centrifuge cup in the above example used for cell centrifugation operations. The cell centrifugation operation is an operation process of cell concentration using the centrifugal sedimentation method.

[0044] Since the centrifugation facility uses the above centrifuge cup, continuous flow concentration of the cell liquid can be realized, and the yield and activity rate of the cell liquid during centrifugation can be improved.

[0045] It is apparent to those skilled in the art that various changes and modifications can be made to the embodiments of the present invention without departing from the spirit and scope of the present invention. Therefore, the present invention is intended to include such changes and modifications when they are within the scope of the claims of the present invention and the scope of equivalent technologies thereof.

Description of Reference Numerals

[0046] 1 Bowl, 2 Cup Cap, 3 First Region Partition Plate, 4 Second Region Partition Plate, 5 Third Region Partition Plate, 6 End Plate, 7 Predetermined Gap, 8 First Drain, 9 Second Drain, 10 Inlet Flow Path, 11 Outlet Flow Path, 12 Inlet, 13 Outlet, 14 Inlet Flow Port, 15 Outlet Flow Port.

Claims

1. A centrifuge cup, comprising: a bowl, a cup cap, a first region partition plate, a second region partition plate, and a third region partition plate; the extending direction of the first region partition plate is from the center of the bowl towards the side wall of the bowl, and there is a predetermined gap between one end towards the side wall of the bowl and the inner wall of the bowl; drains are respectively formed between the outer end of the second region partition plate and the inner wall of the bowl and between the outer end of the third region partition plate and the inner wall of the bowl; an inlet flow path and an outlet flow path are installed in the cup cap and / or the bowl; the inlet flow port of the inlet flow path is located within the predetermined gap; the distance between the inlet flow path and the inner wall of the bowl is within a first predetermined range; the distance between the outlet flow port of the outlet flow path and the inner wall of the bowl is within a second predetermined range; and the outlet flow path is located between the second region partition plate and the third region partition plate. The centrifuge cup is characterized by the above.

2. The fact that there is a predetermined gap between one end towards the side wall of the bowl and the inner wall of the bowl as described above includes that an end plate is installed at one end towards the side wall of the bowl, and a gap is formed between the end plate and the inner wall of the bowl. The centrifuge cup according to Claim 1.

3. The end plate is in an arc shape, and the arc length is 5 mm or more. The centrifuge cup according to Claim 2.

4. The first predetermined range is 0.1 mm to 20 mm. The centrifuge cup according to Claim 1.

5. The second predetermined range is 4 mm or more. The centrifuge cup according to Claim 1.

6. The first region partition plate, the second region partition plate, and the third region partition plate are installed with gaps in sequence along the rotation direction of the bowl. The first region partition plate, the second region partition plate, and the third region partition plate each extend along the radial direction of the bowl and are distributed in a Y shape. The first region partition plate is fixedly connected to the cup cap or the bowl where the inlet flow path is installed, and the second region partition plate and the third region partition plate are fixedly connected to the cup cap or the bowl. The centrifugal cup according to claim 1, characterized in that.

7. The drain formed between the outer end of the second region partition plate and the inner wall of the bowl, and the drain formed between the outer end of the third region partition plate and the inner wall of the bowl are each smaller than the distance between the inlet flow port and the inner wall of the bowl. The centrifugal cup according to claim 1, characterized in that.

8. The second region partition plate and the third region partition plate are distributed on both sides of the outlet flow port. The centrifugal cup according to claim 6, characterized in that.

9. A cell centrifugation method, Performing a cell centrifugation operation with the centrifugal cup according to any one of claims 1 to 8, The cell centrifugation method is, A step of introducing a liquid to be treated from an inlet flow port, A step of circulating and centrifuging the liquid to be treated from the periphery of a predetermined gap to a drain by rotationally driving the centrifugal cup connected to the rotating device by the rotating device, A step of extracting, from the outlet flow port, an outflow liquid which is a liquid present in the vicinity of the outlet flow port after the liquid to be treated has been centrifuged during centrifugation. A cell centrifugation method, characterized by including.

10. A centrifugation facility comprising the centrifugal cup according to any one of claims 1 to 8.

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