Method for evaluating the health of a river ecosystem based on specific responses of microbiota

By leveraging microbial community responses, the method addresses biased evaluation of river health by establishing a scientific index system for assessing ecosystem health through microbial sensitivity classification, enabling accurate and objective pollutant indication.

JP2025522119AActive Publication Date: 2025-07-10GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Patent Information

Application Number
JP2025501822
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2022-08-16
Filing Date
2022-09-21
Publication Date
2025-07-10
Estimated Expiration
2042-09-21

AI Technical Summary

Technical Problem

Existing methods for evaluating river ecosystem health rely heavily on expert knowledge and experience, leading to biased and random evaluation results due to limited understanding of microbial community responses to environmental changes.

Method used

A method utilizing microbial community responses to environmental changes, involving sampling, water quality measurement, microbial DNA extraction, bacterial 16S rRNA gene sequencing, and sensitivity classification to establish a scientific index system for evaluating river ecosystem health.

Benefits of technology

The method provides a direct, objective, and accurate assessment of river ecosystem health by identifying sensitive microbial taxa that indicate characteristic pollutants, applicable to urban river ecosystems.

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Abstract

The present invention discloses a method for evaluating the soundness of a river ecosystem based on the specific response of a microbial community. This method includes the steps of: a: setting sampling points in rural, suburban, and urban central areas along the river respectively; b: measuring 12 indicators including pH, water temperature (WT), dissolved oxygen (DO), chemical oxygen demand (COD), total nitrogen (TN), total organic carbon, nitrate nitrogen, ammonium nitrogen, nitrite nitrogen, total phosphorus, sulfide, and fluoride, and calculating the water quality index WQI; c: extracting the DNA of aquatic microorganisms and performing bacterial 16s rRNA amplicon sequencing; d: obtaining a genus-level information table and screening effective genus information; e: performing sensitivity classification of microorganisms based on an ecological niche model; f: analyzing the reliability of the results. This method can quickly, accurately, and objectively reflect the health status of the urban river ecosystem.
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Citation Information

Patent Citations

  • Urban inland river ecosystem health assessment method based on weights

    CN109063962A

  • Plateau river ecological health evaluation method based on random forest optimization microbial index

    CN110675036A

  • River ecosystem health evaluation method based on co-occurrence network

    CN114707786A

  • METHOD OF DETECTING AND QUANTITATING MICROORGANISM HAVING SPECIFIC FUNTION AND ITS GENE FROM NATURAL ENVIRONMENT, NOVEL 16SrRNA GENE DATA AND PROBES

    WO2001014587A1