Multispecific antibodies and methods of use thereof

JP2025532897APending Publication Date: 2025-10-03DREN BIO INC
View PDF 6 Cites 0 Cited by

Patent Information

Application Number
JP2025518243
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2022-09-28
Filing Date
2023-09-27
Publication Date
2025-10-03

AI Technical Summary

Technical Problem

Existing multispecific antibodies face challenges in promoting proper pairing between heavy and light chains, particularly in designing stable, non-immunogenic, and high-affinity antigen-binding sites that do not pair with other IgG1-4 domains.

Method used

The use of antibody CH4 domains with specific amino acid substitutions to promote stable heavy chain:light chain pairing and heavy chain:heavy chain association, utilizing IgM or IgA domains with high structural similarity to the CH1:CL interface, reduces the likelihood of adverse pairing with Fc region domains.

Benefits of technology

This approach results in stable multispecific antibodies suitable for manufacturing, with improved heavy chain:light chain pairing and reduced immunogenicity, enabling effective targeting of multiple antigens.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure 2025532897000001_ABST
    Figure 2025532897000001_ABST
Patent Text Reader

Abstract

The present disclosure relates to multispecific antibodies, as well as related polynucleotides, vectors, host cells, pharmaceutical compositions, methods of use, and methods of making thereof. In some embodiments, the multispecific antibodies use antibody CH4 domains that contain at least one amino acid substitution that promotes association between the antibody CH4 domains and favors proper heavy chain:light chain pairing.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • US10,982,008

  • Multispecific antibodies

    US20170129962A1

  • Multispecific Antibodies Facilitating Selective Light Chain Pairing

    US20180057567A1

  • Bispecific antibodies

    US20200123260A1

  • Novel bispecific polypeptide complexes

    US20200283524A1