A method for producing red algae using finely fragmented discoids of red algae

Abstract

To provide a method for producing red algae by obtaining seeds of red algae without newly collecting spores and using the seeds. [Solution] Disc callus obtained by irradiating fragmented discs of red algae with blue monochromatic light and culturing them in suspension; germinated disc callus obtained by irradiating said disc callus with light other than blue monochromatic light and culturing them in suspension; germinated disc fragments obtained by irradiating fragmented discs of red algae with light other than blue monochromatic light and culturing them in suspension, and said disc callus; said germinated disc callus, said germinated disc fragments, and fragmented discs of red algae with blue monochromatic light or A method for producing red algae, characterized in that at least one of the following types of germinated attached discs is obtained by irradiating attached discs obtained by irradiating them with light other than monochromatic blue light and culturing them in an attached state, and then irradiating them with light other than monochromatic blue light; or germinated attached discs obtained by irradiating attached discs obtained by irradiating the disc callus with monochromatic blue light or light other than monochromatic blue light and culturing them in an attached state, and then irradiating them with light other than monochromatic blue light, as seeds for the red algae.

Description

[Technical Field]

[0001] The present invention relates to a method for producing red algae using finely fragmented discoids of red algae. [Background technology]

[0002] There are several known methods for cultivating seaweed: (1) collecting spores from seaweed, attaching them to a substrate (Cremona thread or rope), and allowing them to sprout and grow in the ocean; (2) cutting off only the tip of the seaweed (where the growth point is) and clamping the tip directly to a rope or similar for cultivation in the ocean; and (3) tearing up the seaweed and placing it in a pond filled with seawater to allow it to grow naturally (extensive cultivation).

[0003] A modified version of the method (1) above is known as a method for cultivating seaweed (Taoyagisou) of the Rhodophyta, a type of red algae (Patent Document 1). Patent Document 1 discloses a method for producing seeds of seaweed of the Rhodophyta, characterized by including a seedling harvesting step in which algae bodies of seaweed of the Rhodophyta order are placed together with a growth substrate in a tank filled with seawater at a temperature of 15 to 22°C, and carpospores and tetraspores released from the algae are attached to the growth substrate while aerating and stirring the seawater.

[0004] However, in this conventional cultivation method, seaweed is allowed to mature (to form tetraspores and carpospores), and the spores released from the seaweed are then attached to a substrate to form discoids and then upright bodies, which are then used as seedlings for cultivation.This requires artificially creating the ``maturation'' step, which is costly (in terms of time and workability). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Patent Publication No. 2008-11734 Summary of the Invention [Problem to be solved by the invention]

[0006] Therefore, an object of the present invention is to provide a method for obtaining red algae seeds without newly collecting spores and for producing red algae using the seeds. [Means for solving the problem]

[0007] As a result of intensive research aimed at solving the above problems, the present inventors discovered that by irradiating small pieces of red algae discs germinated from tetraspores or carpospores with light of a specific wavelength and culturing them under specific conditions, they can be grown without forming upright bodies (without germination), and that the formation of upright bodies can be induced from the grown disc fragments or discs, thereby completing the present invention. Furthermore, they discovered that by using these as red algae seedlings, red algae (mature bodies) can be efficiently produced without the need to newly collect tetraspores or carpospores, thereby completing the present invention.

[0008] That is, the present invention is as follows. [1] Disc callus obtained by irradiating small pieces of red algae discs with monochromatic blue light and culturing them in suspension. [2] Germinated disc callus obtained by irradiating the disc callus described in [1] with light other than monochromatic blue light and then subjecting it to suspension culture. [3] Germinated disc fragments obtained by irradiating the fragmented discs of red algae with light other than blue monochromatic light and culturing them in suspension. [4] A method for producing disc callus, which comprises irradiating small pieces of red algae discs with blue monochromatic light and culturing them in suspension. [5] A method for producing germinated disc callus, characterized by irradiating the disc callus described in [1] with light other than blue monochromatic light and culturing the resulting germinated disc callus in suspension, and then culturing the germinated disc callus with light other than blue monochromatic light. [6] A method for producing sprouted disc fragments, comprising: irradiating the fragmented discs of red algae with light other than blue monochromatic light and culturing them in suspension. [7] A method for producing attached discoids, characterized in that the discoids are obtained by irradiating fragments of red algae with blue monochromatic light or light other than blue monochromatic light and culturing them in an attached state. [8] A method for producing germinated adherent discs, characterized in that the adherent discs obtained by attaching and culturing fragmented red algae discs under irradiation with blue monochromatic light or light other than blue monochromatic light are then irradiated with light other than blue monochromatic light and then subjected to attachment and culturing. [9] A method for producing adherent discoids, characterized in that the discoid callus according to [1] is cultured in an adherent manner under irradiation with monochromatic blue light or light other than monochromatic blue light.

[10] A method for producing germinated adherent discs, characterized in that the adherent discs obtained by culturing the disc callus described in [1] under exposure to blue monochromatic light or light other than blue monochromatic light are then irradiated with light other than blue monochromatic light and cultured under exposure to the adherent discs.

[11] A method for producing red algae, characterized in that at least one of the following is used as a seedling for the red algae: the disc callus described in [1], the germinated disc callus described in [2], the germinated disc strips described in [3], the germinated attached discs obtained by irradiating fragmented discs of red algae with blue monochromatic light or light other than blue monochromatic light and culturing the attached discs, and then irradiating the attached discs with light other than blue monochromatic light and culturing them in an adherent manner; and the germinated attached discs obtained by irradiating the disc callus described in [1] with blue monochromatic light or light other than blue monochromatic light and culturing them in an adherent manner and then irradiating the attached discs with light other than blue monochromatic light. [Effects of the Invention]

[0009] The discoid callus, germinated discoid callus, and germinated discoid fragments of the present invention are based on discoids and are obtained by artificially controlling the conditions during cultivation, so they can be supplied stably without being affected by natural conditions.

[0010] Furthermore, the method for producing red algae of the present invention is an excellent method for efficiently producing red algae (mature bodies) without the need to newly collect tetraspores or carpospores by using at least one of the above-mentioned disc callus, germinated disc callus, germinated disc fragments, germinated attached discs obtained by attaching and culturing attached discs obtained by irradiating shredded red algal discs with monochromatic blue light or light other than blue light and then irradiating the attached discs with monochromatic ... as seeds. [Brief explanation of the drawings]

[0011] [Figure 1] FIG. 1 is a graph showing the relationship between the number of days of culture and the upright formation rate (vertical axis: %) in Example 1. [Figure 2] 1 is a photograph showing the appearance of discoids after culture in Example 1 (the scale bar in the figure is 100 μm). [Figure 3] 1 is a photograph showing the appearance of discoid callus after culturing in Example 2 (the scale bar in the figure is 200 μm). [Figure 4] 1 is a photograph showing the appearance of germinated disc callus after culturing in Example 3 (the scale bar in the figure is 200 μm). [Figure 5] 1 shows a diagram illustrating the relationship between the number of days of culture and the rate of erect body formation (vertical axis: %) in Example 5, and photographs of the appearance of discoids before and after culture (the scale bar in the diagram is 200 μm). [Figure 6] 1 shows a diagram illustrating the relationship between the number of days of culture and the rate of erect body formation (vertical axis: %) in Example 6, and photographs of the appearance of discoids before and after culture (the scale bar in the diagram is 500 μm). DETAILED DESCRIPTION OF THE INVENTION

[0012] The discoid callus of the present invention is obtained by irradiating small pieces of red algae discoids with blue monochromatic light and then subjecting them to suspension culture.

[0013] The red algae that can be used as the source of discoid callus in the present invention are those that have discoids in their life cycle, and do not include those that do not have discoids in their life cycle. Examples of red algae that have discoids in their life cycle include those in the order Acrocephalales, such as A. sieboldii; those in the order Gracilariales, such as Gracilaria sieboldii; those in the order Gigarinales, such as A. sieboldii, A. nigricans ...

[0014] The method for fragmenting red algae discoids is not particularly limited, but examples include crushing using a mixer, mill, etc. After crushing, the discoids are preferably sieved using a plankton net, metal sieve, etc. to ensure that the discoid fragments are of a uniform size. The size of the red algae discoid fragments is not particularly limited, but is, for example, 10 to 500 mm, preferably 50 to 200 mm. This size refers to the length of the long side.

[0015] The strips of red algae discoids are cultured in suspension under blue monochromatic light irradiation. Monochromatic blue light here refers to light capable of irradiating wavelengths in the blue range of 430 to 490 nm. Examples of light sources capable of irradiating such blue monochromatic light include LEDs. The conditions for irradiating the blue monochromatic light are not particularly limited, but for example, it may be irradiated from above the culture vessel. It goes without saying that when irradiating this blue monochromatic light, light of other wavelengths must be prevented from entering. The illuminance is a value measured at the surface of the culture vessel.

[0016] The conditions for the suspension culture under irradiation with blue monochromatic light are not particularly limited, except that the platelets are irradiated with blue monochromatic light to float (not adhere), and for example, the water temperature is 15 to 30°C, preferably 24 to 26°C, the illuminance is 1000 to 5000 lux, preferably 2000 to 3000 lux, the light and dark periods are 10 hours:14 hours or 14 hours:10 hours, preferably 12 hours:12 hours, and the culture is performed in artificial seawater, seawater, PES culture medium (Taizo Motomura, "Culture Method (3) Preparation of Culture Medium (Seaweed)", "Algology"). A culture solution such as that in "Experiments and Practice" (eds. Ariga Yukatsu, Inoue Isao, Tanaka Jiro, Yokohama Yasutsugu, and Yoshida Tadao), Kodansha, Tokyo, 2000; 170-171) can be placed in a container, such as a flask, at least part of which, preferably the entire container, is light-transmitting, and suspension culture can be performed while stirring, shaking, aerating, etc., to prevent the discoid bodies from adhering to the container. The number of days for culture is not particularly limited, but is preferably 12 to 60 days, and more preferably 14 to 30 days.

[0017] By culturing in this manner, the discoid fragments undergo cell division to become discoid callus. The size of this discoid callus is not particularly limited, but is, for example, 10 to 500 μm, preferably 50 to 200 μm. This size is the length of the long side.

[0018] This discoid callus can be re-fragmented and cultured under monochromatic blue light to produce discoid callus, allowing for indefinite proliferation. Furthermore, if the discoid callus is derived from a single disc, it is possible to obtain genetically uniform clones.

[0019] The discoid callus is specified by the production method, but this is because it cannot be specified by structure, composition, or other physical properties.

[0020] Furthermore, adherent discs can be obtained by irradiating the disc callus with monochromatic blue light or light other than monochromatic blue light and culturing it under adherent conditions. When disc callus is irradiated with monochromatic blue light and cultured under adherent conditions, growth without upright growth occurs. When disc callus is irradiated with light other than monochromatic blue light and cultured under adherent conditions, normal disc growth occurs. Furthermore, when adherent discs are irradiated with light other than monochromatic blue light and cultured under adherent conditions, germinated adherent discs, which form upright discs, are obtained. The adherent discs and germinated adherent discs are similar to conventional discs and germinated adherent discs. Unlike suspension culture, adherent culture refers to a culture in which disc callus adheres to a container or the like by standing without stirring, shaking, aeration, etc. during culture. Other culture conditions are the same as those described above. After disc callus adherence, stirring, shaking, aeration, etc. may be performed.

[0021] On the other hand, the germinated discoid callus of the present invention can be obtained by irradiating the discoid callus obtained as described above with light other than monochromatic blue light and culturing it in suspension. Here, "light other than monochromatic blue light" refers to light other than the monochromatic blue light used in the suspension culture of the discoid callus. Specific examples include red light, green light, white light (a combination of blue, red, and green light), fluorescent light, sunlight, etc.

[0022] The conditions for floating culture of discoid callus by irradiating it with light other than the above-mentioned monochromatic blue light are not particularly limited as long as they allow floating culture of discoid callus, and are similar to those for floating culture of the above-mentioned discoid pieces.

[0023] By this suspension culture, the disc callus germinates to form an upright germinated disc callus. The size of this germinated disc callus is not particularly limited, but is, for example, 500 μm to 3 mm, preferably 1 to 2 mm. This size is the length of the long stem.

[0024] The germinated disc callus is specified by the production method, but this is because it cannot be specified by structure, composition, or other physical properties.

[0025] Furthermore, by irradiating strips of red algae with light other than monochromatic blue light and culturing them in suspension, germinated strips of red algae can be obtained, in which the strips germinate and form upright bodies, as described above. The red algae strips and other culture conditions are the same as those described above.

[0026] The size of the germinated disc pieces is not particularly limited, but is, for example, 200 μm to 2 mm, preferably 300 μm to 1.5 mm. This size is the length of the long edge.

[0027] The germinated disc pieces are specified by the manufacturing method, but this is because they cannot be specified by structure, composition, or other physical properties.

[0028] Furthermore, adherent discoids can be obtained by irradiating strips of red algae discoids with light other than monochromatic blue light and culturing them in an adherent culture. Furthermore, if these adherent discoids are irradiated with light other than monochromatic blue light and cultured in an adherent culture, sprouted adherent discoids, in which upright bodies form from the discoids, can be obtained. These adherent discoids and sprouted adherent discoids are similar to conventional discoids and sprouted discoids. The red algae discoid fragments and other culture conditions are the same as those described above.

[0029] Furthermore, adherent discoids can be obtained by irradiating strips of red algae discoids with blue monochromatic light or blue monochromatic light and culturing them in an adherent culture. When strips of red algae discoids are irradiated with blue monochromatic light and cultured in an adherent culture, they can grow without forming an upright body, while when strips of red algae discoids are irradiated with light other than blue monochromatic light and cultured in an adherent culture, they grow into normal discoids. Furthermore, when these adherent discoids are irradiated with light other than blue monochromatic light and cultured in an adherent culture, sprouted adherent discoids, in which an upright body forms from the discoids, can be obtained. These adherent discoids and sprouted adherent discoids are similar to conventional discoids and sprouted discoids. The red algae discoids and other culture conditions are the same as those described above.

[0030] The disc callus, germinated disc callus, germinated disc fragments, attached discs, and germinated attached discs of the present invention obtained as described above can be used for the collection of red algae seedlings, the collection of active ingredients contained in the discs, etc. Among these, the disc callus, germinated disc callus, and germinated disc fragments of the present invention are preferably used, and the disc callus and germinated disc callus of the present invention are more preferably used in terms of efficiency, etc.

[0031] When the disc callus, germinated disc callus, germinated disc fragments, attached discs, or germinated attached discs of the present invention are used as red algae seedlings, any of the conventionally known red algae cultivation methods can be used, except that the disc callus, germinated disc callus, germinated disc fragments, attached discs, or germinated attached discs of the present invention are used as red algae seedlings. Such cultivation methods include, for example, floating culture in land-based aquariums, rope culture in which the discs are attached to a substrate to promote the formation of upright bodies, and extensive culture in which the upright bodies are spread in seawater ponds, allowed to grow, and then harvested.

[0032] By culturing the seedlings of the present invention by these culturing methods for 60 to 90 days, preferably 70 to 80 days, mature plants can be produced.

[0033] The red algae (mature bodies) obtained in this manner can be eaten as they are in seaweed salads, as a garnish for sashimi, or can be processed into agar ingredients, tsukudani (simmered food), dried red algae, etc., just like conventional red algae.

[0034] Furthermore, when useful components are extracted from the disc callus, germinated disc callus, germinated disc fragments, attached discs, and germinated attached discs of the present invention, conventional extraction methods can be used depending on the desired useful component. Useful components include carrageenan. [Example]

[0035] The present invention will be described in detail below by way of examples, but the present invention is not limited to these examples in any way.

[0036] Example 1 Effect of light type on discoid suspension culture: Discs of Acanthus japonica (Methoxyles) were placed one per culture vessel (24 locations) containing 1 mL of PES medium. The light source was a blue monochromatic light (B: wavelength 445 nm; Nippon Medical and Chemical Instruments Manufacturing Co., Ltd., 3-in-1 LED; also used in the following examples), red monochromatic light (R: wavelength 660 nm), green monochromatic light (G: wavelength 520 nm), or white light (W: mixed light of wavelengths 660 nm, 520 nm, and 445 nm) from an LED or fluorescent lamp (FL). The light was irradiated from above the culture vessel at an illuminance of 2500 lux, with a 12:12-hour light:dark cycle, at a water temperature of 26-24°C, and the suspension culture was performed with shaking for 17 days. During culture, no light other than the above was allowed to enter.

[0037] The formation of upright bodies from the discs was confirmed under a microscope every day of culture. The number of days in culture and the percentage of upright bodies formed from the discs (upright body formation rate (vertical axis: %)) are shown in Figure 1. Figure 2 shows a photograph of the appearance of the discs after 17 days of culture.

[0038] Even after 17 days of culture, when cells were exposed to monochromatic blue light and cultured in suspension, no upright bodies were formed from the discoids, but the size of the discoids increased. On the other hand, when cells were exposed to monochromatic red and green light, a mixture of white light, and fluorescent light, upright bodies were formed from the discoids, although at different times.

[0039] Example 2 Suspension culture of discoid fragments: The discoids of Acanthus sieboldii (Pyralidae) were crushed in a blender and then sieved through 200 μm and 50 μm plankton nets to obtain discoid fragments. Approximately 100,000 of these fragments were placed in a culture vessel containing 200 mL of culture medium. The vessel was irradiated from the bottom with monochromatic blue light (B: wavelength 445 nm) at an illuminance of 2500 lux, with a 12:12-hour light / dark cycle, at a water temperature of 24-26°C, and cultured with shaking for 14 days. A photograph of the appearance after culture is shown in Figure 3.

[0040] When the discoid fragments were irradiated with monochromatic blue light and cultured in suspension, cell division occurred, forming discoid calli, the size (long side) of which was approximately 100-200 μm.

[0041] Example 3 Discoid callus suspension culture: Five hundred discoid calli obtained in Example 2 were placed in a culture vessel containing 200 mL of culture solution, and the vessels were irradiated with white light from above at an illuminance of 5,000 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 19-21°C, and cultured in suspension for 14 days with shaking. A photograph of the appearance after culture is shown in Figure 4.

[0042] When the discoid callus was cultured under white light (light other than blue monochromatic light), it germinated and formed an upright germinated discoid callus, with the size (long side) of about 1 to 2 mm.

[0043] Example 4 Red algae (mature) production: Fifty germinated disc-shaped calli obtained in Example 3 were placed in a culture vessel containing 1 L of culture medium, and white light was irradiated from the top of the culture vessel at an illuminance of 5000 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 19-21°C, and cultured with aeration, resulting in the production of mature Taoyagisou plants.

[0044] Example 5 Discoid suspension culture: A single disc of Gracilaria spp. (Gracilariales) was placed in a culture vessel containing 1 mL of culture medium, and a blue monochromatic light (B: wavelength 445 nm) or white light (W: mixed light of wavelengths 660 nm, 520 nm, and 445 nm) LED was used as the light source. This was irradiated from above the culture vessel at an illuminance of 2500 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 25°C, and the vessel was cultured in suspension for 14 days with shaking. When using white light, the vessel was also cultured at a water temperature of 20°C.

[0045] The formation of upright bodies from the discs was examined under a microscope every 7 days of culture. The number of days of culture and the percentage of upright bodies formed from the discs (upright body formation rate (vertical axis: %)) are shown in Figure 5. Figure 5 also shows a photograph of the appearance of the discs after 14 days of culture.

[0046] Similar to T. sieboldii, when exposed to monochromatic blue light and cultured in suspension, the formation of upright bodies from discoids was not observed, whereas when exposed to white light, the formation of upright bodies from discoids was observed at both culture temperatures.

[0047] Example 6 Discoid suspension culture: A single centipede (Gigartinales) disc was placed in a culture vessel containing 2 mL of culture medium, and the vessel was irradiated from above with a blue monochromatic light (B: wavelength 445 nm) or white light (W: mixed light of wavelengths 660 nm, 520 nm, and 445 nm) LED as the light source, with an illuminance of 2500 lux and a 12-hour light:12-hour dark cycle. The vessel was cultured in suspension for 14 days with shaking at a water temperature of 25°C. When using white light, the vessel was also cultured at a water temperature of 20°C.

[0048] The formation of upright bodies from the discs was examined under a microscope every 7 days of culture. The number of days of culture and the percentage of upright bodies formed from the discs (upright body formation rate (vertical axis: %)) are shown in Figure 6. Figure 6 also shows a photograph of the appearance of the discs after 14 days of culture.

[0049] Like C. japonica, when the centipede was exposed to monochromatic blue light and cultured in suspension, no upright bodies were formed from the discs, whereas when exposed to white light, upright bodies were formed from the discs at both culture temperatures.

[0050] Example 7 Suspension culture of discoid fragments: Strips of discoids of Acanthus japonica (Acanthiales) were obtained in the same manner as in Example 2. Five hundred of these strips were placed in a culture vessel containing 200 mL of culture solution, and the vessel was irradiated from above with white light at an intensity of 5000 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 19-21°C, and aerated for 14 days in suspension culture.

[0051] When the disc fragments were irradiated with white light (light other than monochromatic blue light) and cultured in suspension, they germinated into upright germinated disc fragments. The size (long side) of these germinated disc fragments was approximately 0.5 mm to 1.0 mm. Furthermore, the shape of these germinated disc fragments was different from that of conventional discs and germinated discs.

[0052] Example 8 Adhesion culture of discoid callus: Five hundred disc-shaped calli obtained in Example 2 were placed in a culture vessel containing 200 mL of culture medium, and white light was irradiated from above the culture vessel at an illuminance of 5000 lux with a 12-hour light:12-hour dark cycle.The water temperature was kept at 19-21°C, and the calli were allowed to stand for 14 days for attachment culture.

[0053] The discoid callus became discoids attached to the culture vessel. These attached discoids were further cultured under the same conditions as above to produce germinated attached discoids. These attached discoids and germinated attached discoids were similar to conventional discoids and germinated discoids.

[0054] Example 9 Adherent culture of discoid strips: Strips of discoids of Acanthus japonica (Acanthales) were obtained in the same manner as in Example 2. These strips were irradiated with blue monochromatic light (B: wavelength 445 nm) from the bottom of the culture vessel at an illuminance of 2500 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 24-26°C, and allowed to stand for 14 days for adherent culture.

[0055] The discoid fragments became adherent discoids. These adherent discoids were further cultured under white light under the same conditions as in Example 8, and developed into germinated adherent discoids. These adherent discoids and germinated adherent discoids were similar to conventional discoids and germinated discoids.

[0056] Example 10 Adherent culture of discoid strips: Strips of discoid bodies of Acanthus japonica (Acanthiales) were obtained in the same manner as in Example 2. These strips were irradiated with white light from above the culture vessel at an illuminance of 5000 lux, with a 12-hour light:12-hour dark cycle, at a water temperature of 19 to 21°C, and allowed to stand for 14 days for attachment culture.

[0057] The discoid fragments became adherent discoids. When these adherent discoids were further cultured under the same conditions as above, they became germinated adherent discoids. These adherent discoids and germinated adherent discoids were similar to conventional discoids and germinated discoids.

[0058] These results indicate that the combination of monochromatic blue light and suspension culture promotes the growth of discoid fragments and discoid callus without promoting germination (formation of upright bodies), while the combination of light other than monochromatic blue light and suspension culture promotes germination from discoid fragments and discoid callus. Furthermore, in the case of adherent culture, any light causes the seeds to revert to their original discoid state, and subsequent irradiation with light other than monochromatic blue light results in germination from normal discoids. [Industrial Applicability]

[0059] The present invention can be used to produce red algae seeds and mature red algae.

Claims

1. Discoid callus is obtained by irradiating small pieces of red algae discoids with blue monochromatic light and culturing them in suspension.

2. A germinated discoid callus obtained by irradiating the discoid callus of claim 1 with light other than monochromatic blue light and then subjecting it to suspension culture.

3. Germinated disc fragments are obtained by irradiating the fragmented discs of red algae with light other than blue monochromatic light and culturing them in suspension.

4. A method for producing discoid callus, which comprises irradiating small pieces of red algae discoids with blue monochromatic light and culturing them in suspension.

5. A method for producing germinated discoid callus, comprising irradiating the discoid callus according to claim 1 with light other than blue monochromatic light and culturing the resulting germinated discoid callus in suspension under light other than blue monochromatic light.

6. A method for producing sprouted disc fragments, comprising: irradiating strips of red algae discs with light other than blue monochromatic light and culturing them in suspension.

7. A method for producing adherent discoids, characterized in that the discoids are obtained by culturing fragments of red algae in adherent culture under irradiation with blue monochromatic light or light other than blue monochromatic light.

8. A method for producing germinated adherent discoids, characterized in that the adherent discoids obtained by culturing fragmented red algae discoids in an adherent culture medium under irradiation with blue monochromatic light or light other than blue monochromatic light are then irradiated with light other than blue monochromatic light and then cultured in an adherent culture medium.

9. A method for producing adherent discoids, which is obtained by culturing the discoid callus of claim 1 under irradiation with blue monochromatic light or light other than blue monochromatic light.

10. A method for producing germinated adherent discs, characterized in that the adherent discs obtained by culturing the disc callus described in claim 1 under exposure to blue monochromatic light or light other than blue monochromatic light are then irradiated with light other than blue monochromatic light and cultured under exposure to the adherent discs.

11. A method for producing red algae, comprising using at least one of the following as seeds for the red algae: the disc callus of claim 1; the germinated disc callus of claim 2; the germinated disc strips of claim 3; germinated attached discs obtained by culturing attached discs obtained by irradiating fragmented discs of red algae with blue monochromatic light or light other than blue monochromatic light and then irradiating the attached discs with light other than blue monochromatic light; and germinated attached discs obtained by culturing attached discs obtained by irradiating the disc callus of claim 1 with blue monochromatic light or light other than blue monochromatic light and then irradiating the attached discs with light other than blue monochromatic light.

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