Endo180 gene expression enhancer

Abstract

The present invention aims to provide a novel Endo180 gene expression promoter. [Solution] The technical features of the present invention for solving the above problems are as follows. 1. An Endo180 gene expression enhancer containing a composition containing GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-bD-glucose) as an active ingredient. 2. An Endo180 gene expression promoter containing a composition with GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-bD-glucose) derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name TRFK306) as the active ingredient.

Description

Technical Field

[0001] The present invention relates to an Endo180 gene expression promoter. In particular, the present invention relates to a topical skin composition or a food or drink containing an Endo180 gene expression promoter.

Background Art

[0002] Collagen is a major component of the extracellular matrix in the skin and plays an important role in maintaining the elasticity and firmness of the skin. As a major component of the extracellular matrix other than collagen, elastin is mentioned. Elastin is also an important factor in maintaining the elasticity of the skin, just like collagen. It is known that collagen and elastin are produced by fibroblasts and their degradation products are absorbed again by fibroblasts in a cycle. However, it is known that an abnormality in this cycle and the accumulation of degradation products cause alterations in cell morphology and an increase in oxidative stress in the skin. Therefore, it is considered very important to keep the production-degradation cycle of collagen and elastin that make up the extracellular matrix normal in order to keep the skin condition normal. Here, since it is known that the endo180 gene is involved in the intracellular uptake of degraded collagen, by expressing the endo180 gene, degraded collagen can be taken into the cell and the skin condition can be kept normal (Patent Document 1).

[0003]

Patent Document 1

Disclosure of the Invention

Problems to be Solved by the Invention

[0004] Against this background, the inventors discovered that a composition containing purple tea and its intrinsic component GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose) has an Endo180 gene expression promoting effect, and thus completed the present invention. In other words, the present invention aims to provide a novel Endo180 gene expression promoter. [Means for solving the problem]

[0005] The technical features of the present invention for solving the above problems are as follows. 1. An Endo180 gene expression enhancer containing a composition containing GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose) as an active ingredient. 2. An Endo180 gene expression promoter containing a GHG-containing composition derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name TRFK306) as the active ingredient. [Brief explanation of the drawing]

[0006] [Figure 1] This is the HPLC chromatogram of the Kenyan purple tea extract (purple tea extract) used in this embodiment. [Figure 2] This graph shows the results of the evaluation of the Endo180 gene expression promoting effect of purple tea extract. [Best Mode for Carrying Out the Invention]

[0007] The present invention will be described in detail below. The Endo180 gene expression promoter of the present invention contains a composition containing GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose) as an active ingredient.

[0008] GHG is a compound represented by the following chemical formula (1). [ka] It is preferable to use a GHG-containing composition derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name TRFK306) as described above. The GHG-containing composition is an extract obtained from Kenyan purple tea and contains GHG in an amount of 0.1 to 99% by mass. Its form may be liquid, solid, semi-solid, or gel-like, and the GHG content in terms of solid content is preferably 0.1 to 99% by mass, but when used as an ingredient in food, beverages, or cosmetics, it is preferable to have a GHG content of 1 to 30% by mass, and more preferably 3 to 10% by mass, in order to improve productivity. In particular, a GHG-containing composition containing GHG in an amount of 1 to 10% by mass can be efficiently obtained by the manufacturing method described below.

[0009] The Kenyan purple tea used as the raw material for the above-mentioned GHG-containing composition is a tea plant developed by the Kenyan government through crossbreeding, and is named the variety TRFK306. The tea leaves of Kenyan purple tea contain anthocyanins, which give it its purple color, hence it is commonly called "purple tea." In addition to TRFK306, other purple teas such as Sunrouge, developed by the National Agriculture and Food Research Organization (NARO), are also known, but Kenyan purple tea contains a high concentration of GHG, a unique component not found in other purple teas.

[0010] The part of the Kenyan purple tea (TRFK306) used in the production of the above GHG-containing composition is not particularly limited; leaves, stems, roots, flowers, seeds, etc., can be used, but the use of leaves is particularly preferred because a higher concentration of GHG can be obtained.

[0011] The above GHG-containing composition can preferably be obtained, for example, by crushing fresh or dried leaves of Kenyan purple tea TRFK306 (hereinafter referred to as "purple tea leaves") and extracting them using a polar solvent (including water; the same applies hereinafter). To improve the extraction efficiency, the purple tea leaves may be subjected to appropriate chemical treatments such as acid or alkali decomposition or enzymatic decomposition before extraction.

[0012] Specifically, the GHG-containing composition can be manufactured by the method described below. Specifically, the purple tea leaves described above, either fresh or dried, are first subjected to chemical treatment such as acid or alkali decomposition or enzymatic decomposition. Citric acid is preferred in this process.

[0013] Then, a polar solvent is added to the purple tea leaves, and the mixture is shaken or heated under reflux to extract GHG into the solvent.

[0014] In this case, the polar solvent is not particularly limited, but water, alcohols, and ketones can be used. These may be used individually or in combination of two or more. In this case, it is preferable to use water.

[0015] As aqueous alcohol-based solvents, aqueous solvents such as ethanol, methanol, and propanol can be used, with aqueous ethanol being particularly preferred. As aqueous ketone-based solvents, aqueous solvents such as acetone, methyl ethyl ketone, diethyl ketone, and chloroacetone can be used, with aqueous acetone being particularly preferred.

[0016] In the method for producing the above-mentioned GHG-containing composition, the heating under reflux can be carried out by a well-known method using the above-mentioned water, aqueous alcohol-based solvent, or aqueous ketone-based solvent. The heating temperature is preferably 30 to 95°C, more preferably 30 to 50°C, and the reflux time is preferably 1 to 4 hours.

[0017] In addition, in the method for producing the above GHG-containing composition, shaking, stirring, etc. may be appropriately carried out as necessary.

[0018] In addition, in the method for producing the above GHG-containing composition, it is preferable to distill off the above solvent under reduced pressure after extraction. According to this, a composition containing no organic solvent can be obtained, and it is possible to conform to the safety standards, etc. as a food material for blending in food and drink products such as functional foods and health foods.

[0019] Furthermore, in the method for producing the above GHG-containing composition, stepwise extraction can be carried out with a plurality of solvents. Thereby, a GHG-containing composition containing GHG at a high concentration can be produced with a higher yield.

[0020] The extract obtained by the above method can be used as it is or concentrated to obtain a GHG-containing composition. Furthermore, it can be made into a powder by freeze-drying or spray-drying to obtain a powdered GHG-containing composition. Also, it is not limited to these forms. Insoluble matters contained in the extract can be appropriately removed by filtration or the like. The insoluble matters may be further pulverized into fine particles.

[0021] Furthermore, the above GHG-containing composition can use an extract of commercially available purple tea TRFK306 produced in Kenya. Specifically, purple tea extract-WSPS, purple tea extract-P, etc. manufactured by Oriza Oil Co., Ltd. can be used.

[0022] The Endo180 gene expression promoter of the present invention can be used in the form of tablets, granules, powders, liquids, powders, granules, capsules, jelly-like agents, etc. (pharmaceuticals or quasi-drugs) by adding a pharmaceutically acceptable base material to the active ingredient thereof, namely, GHG or a GHG-containing composition, as necessary. Furthermore, the active ingredient of the present invention, GHG or a GHG-containing composition, can be used as a material for various food compositions, beverage compositions, and cosmetic compositions. These compositions refer to those obtained by combining ingredients suitable for their respective uses through some technical means.

[0023] Examples of the food and beverage compositions according to the present invention include general foods such as edible oils (salad oil), confectionery (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummies, tablets, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, and beverages (juice, coffee, black tea, tea, carbonated drinks, sports drinks, etc.), as well as health foods (tablets, capsules, etc.) and nutritional supplements (nutritional drinks, etc.). The active ingredient of the present invention (GHG or GHG-containing composition) may be appropriately blended into these food and beverage compositions.

[0024] The food and beverage compositions according to the present invention can contain various components depending on their type. For example, food materials such as glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, sucrose fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorants, flavorings, and preservatives can be used.

[0025] Furthermore, food and beverage compositions with health-maintaining functions may contain other antioxidants and health food ingredients, (for example, antioxidants, reduced ascorbic acid (vitamin C), vitamin E, reduced glutathione, tocotrienol, vitamin A derivatives, lycopene, β-cryptoxanthin, astaxanthin, zeaxanthin, fucoxanthin, uric acid, ubiquinone, coenzyme Q10, folic acid, garlic extract, allicin, sezamin, lignans, catechin, isoflavone, chalcone, tannins, flavonoids, coumarin, isocoumarins, blueberry extract, arbutin, tannin, anthocyanin, apple polyphenols, grape seed extract, elladic acid, kojic acid, surge extract, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin V, choline, niacin, Pantothenic acid, calcium folate, EPA, oligosaccharides, dietary fiber, squalene, soy lecithin, taurine, Dunaliella, protein, octacosanol, DHA, egg yolk lecithin, linoleic acid, lactoferrin, magnesium, zinc, chromium, selenium, potassium, heme iron, oyster extract, chitosan, chitin oligosaccharides, collagen, chondroitin, turmeric, licorice, goji berry, cinnamon, hawthorn, ginger, reishi mushroom, Clam extract, soft-shelled turtle, plantain, chamomile, dandelion, hibiscus, honey, bollen, royal jelly, lime, lavender, rosehip, rosemary, sage, bifidobacteria, Enterococcus faecalis, Lacris, wheat germ oil, sesame oil, perilla oil, soybean oil, medium-chain fatty acids, Agaricus, ginkgo leaf extract, turmeric, chondroitin, brown rice germ extract, reishi mushroom, onion, DHA, EPA, DPASweet tea, Cordyceps sinensis, garlic, bee larvae, papaya, Pu-erh tea, propolis, Megusuri tree, Yabushitake mushroom, royal jelly, saw palmetto, hyaluronic acid, collagen, GABA, harp seal oil, shark cartilage, glucosamine, lecithin, phosphatidylserine, Panax notoginseng, mulberry leaves, soybean extract, echinacea, Eleutherococcus senticosus, barley extract, olive leaves, olive fruit, Gymnema sylvestre, Banaba, Salacia, Garcinia cambogia, chitosan, St. John's wort, jujube, carrot, Passionflower, broccoli, placenta, Job's tears, grape seeds, peanut husks, bilberry, black cohosh, milk thistle, bay leaf, sage, rosemary, rhodiola, black vinegar, bitter melon, maca, safflower, flax, oolong tea, flower thorns, caffeine, capsaicin, xylooligosaccharides, glucosamine, buckwheat, citrus, dietary fiber, protein, prune, spirulina, barley grass, nucleic acids, yeast, shiitake mushroom, plum paste, amino acids, deep-sea shark extract, noni, oyster meat, soft-shelled turtle, shiitake mushrooms Champignon, plantain, acerola, pineapple, banana, peach, apricot, melon, strawberry, raspberry, orange, fucoidan, Phellinus linteus, cranberry, chondroitin sulfate, zinc, iron, ceramide, silk peptide, glycine, niacin, chaste tree, ceramide, L-cysteine, L-carnitine, red wine leaf, millet, horsetail, biotin, Centella asiatica, haskap, pycnogenol, butterbur, rhubarb, clove, rosemary Ingredients such as catechin, pu-erh tea, citric acid, brewer's yeast, melilot, black ginger, ginger, zedoary, nattokinase, red yeast rice, tocotrienol, lactoferrin, cinnamon, buckwheat, cocoa, yuzu seed extract, perilla seed extract, lychee seed extract, evening primrose extract, black rice extract, alpha-lipoic acid, GABA, green coffee bean extract, butterbur extract, kiwi seed extract, Satsuma mandarin extract, red ginger extract, and astaxanthin can also be included.

[0026] Specifically, the active ingredient of the present invention (GHG or GHG-containing composition) can be spray-dried or freeze-dried as is, or, in the case of an extract, together with powdered dextrin. This can then be easily incorporated into food products (such as instant foods) by processing it into powder, granules, tablets, or solutions. Furthermore, it is possible to mix it with a binder such as gum arabic to form a powder or granules, which can then be added to solid foods.

[0027] When the Endo180 gene expression promoter of the present invention is to be made into a pharmaceutical product (including pharmaceuticals and quasi-drugs), it can be manufactured by appropriately blending the active ingredient of the present invention (GHG or a GHG-containing composition) with raw materials for pharmaceutical formulations. The above-mentioned pharmaceutical product may be used in humans or in organisms other than humans (such as mammals). Examples of formulation ingredients that can be incorporated into the above-mentioned drugs include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hydrogenated vegetable oil, kaolin, talc, etc.), binders (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrants (sodium alginate, agar, sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, monoglyceride stearate, starch, lactose, acacia powder, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (quaternary ammonium base, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), and lubricants (purified talc, stearate, polyethylene glycol, etc.).

[0028] The above-mentioned drugs can generally be administered orally in the form of tablets, pills, soft or hard capsules, granules, powders, or granules. Water-soluble preparations can also be administered orally as liquids. Furthermore, parenteral administration is also possible. When administered parenterally, the composition can be dispersed in a suitable solubilizer such as ethanol or water, and then applied in the form of poultices, lotions, ointments, tinctures, or creams. Water-soluble preparations of the composition can also be applied as is, or with the addition of dispersants, suspensions, stabilizers, etc., in the form of poultices, lotions, ointments, tinctures, or creams.

[0029] The dosage may vary depending on the method of administration, the patient's condition, and the patient's age, but adults can usually be given 5 to 200 mg of the active ingredient per day, while children can usually be given 0.5 to 100 mg.

[0030] The mixing ratio when using the active ingredient of the present invention (GHG or GHG-containing composition) as the above-mentioned pharmaceutical can be appropriately changed depending on the dosage form, but it is generally appropriate to use about 0.01 to 10 wt% when administered orally or by mucosal absorption, and about 0.01 to 20 wt% when administered parenterally. Note that the dosage will vary depending on various conditions, so it may be sufficient to use a smaller amount than the above dosage, or it may be necessary to administer a dose exceeding the range. In addition to the present composition, the pharmaceutical composition may also contain other known compounds commonly used in the pharmaceutical field, and compounds necessary for molding into a form suitable for oral administration. Examples of such compounds include lactose, starch, hydroxypropyl cellulose, kaolin, talc, and calcium carbonate.

[0031] Examples of the cosmetic composition of the present invention include emulsions, soaps, facial cleansers, bath additives, creams, lotions, toners, colognes, shaving creams, shaving lotions, cosmetic oils, sunscreen lotions, face powders, foundations, perfumes, face masks, nail creams, nail polish, nail polish removers, eyebrow pencils, blushes, eye creams, eyeshadows, mascaras, eyeliners, lipsticks, lip balms, shampoos, conditioners, hair dyes, dispersions, and cleansing agents.

[0032] In addition to the active ingredient of the present invention (GHG or GHG-containing composition), the above-described cosmetic composition may contain, to the extent that it does not impair the effects of the active ingredient, other ingredients used in topical skin preparations such as cosmetics and quasi-drugs, oils, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc. Examples are listed below, but the present invention is not limited to these examples.

[0033] (1) Examples of oil content Ester-based oil phase components: Glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diethyl adipate Sopropyl, Isoarachil Neopentanoate, Caprylic / Capric Triglyceride, Trimethylolpropane Tri-2-ethylhexanoate, Trimethylolpropane Triisostearate, Pentaerythritol Tetra-2-ethylhexanoate, Cetyl Caprylate, Decyl Laurate, Hexyl Laurate, Decyl Myristate, Myristyl Myristate, Cetyl Myristate, Stearyl Stearate, Decyl Oleate, Cetyl Ricinoleate, Isostearyl Laurate, Isotridecyl Myristate, Isocetyl Myristate, Iso Tearyl, Isocetyl Palmitate, Isostearyl Palmitate, Octyl Stearate, Isocetyl Stearate, Isodecyl Oleate, Octyldodecyl Oleate, Octyldodecyl Linoleate, Isopropyl Isostearate, Cetostearyl 2-Ethylhexanoate, Stearyl 2-Ethylhexanoate, Hexyl Isostearate, Ethylene Glycol Dioctanoate, Ethylene Glycol Dioleate, Propylene Glycol Dicaprate, Di(Capryl / Capric Acid)Propylene Glycol, Propylene Glycol Dicaprylate, Dicaprin Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate,Dialkyl (C12-18) carbonate, triisocetyl citrate, triisoarachil citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate Examples include ropil, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate. Hydrocarbon-based oil phase components include squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and naturally derived waxes: animal oils and their hydrogenated oils such as beef tallow, hydrogenated beef tallow, pork tallow, hydrogenated pork tallow, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, Examples include hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, meadowfoam oil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, hydrogenated coconut oil, and other vegetable oils and their hydrogenated oils, beeswax, high-acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, montan wax, and other waxes. Silicone-based oil phase components include dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, silicone RTV rubber, etc. Examples of fluorine-based oil phase components include perfluoropolyethers, fluorine-modified organopolysiloxanes, fluorinated pitch, fluorocarbons, fluoroalcohols, and fluoroalkyl / polyoxyalkylene copolymerized organopolysiloxanes.

[0034] (2) Examples of higher alcohols Examples include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0035] (3) Examples of fatty acids Examples include caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0036] (4) Examples of UV absorbers Para-aminobenzoic acid, amyl para-aminobenzoate, ethyl dihydroxypropyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyl dimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl mono-2-ethylhexanoate dipara-methoxycinnamate, isopropyl para-methoxycinnamate, diethanolamine para-methoxyhydrocinnamate salt, diisopropyl / diisopropyl cinnamic acid ester mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, Examples include dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and their polymer derivatives and silane derivatives.

[0037] (5) Examples of powders and pigments Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake, Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polymers such as polyethylene powder, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, Prussian blue, zinc oxide, titanium dioxide, acid Examples include white pigments such as cerium oxide, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium mica, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular restrictions on the shape (spherical, rod-shaped, needle-shaped, plate-shaped, irregular shape, flake-shaped, spindle-shaped, etc.) and particle size of these powders. These powders may or may not have been pre-treated by conventional surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil treatment, N-acylated lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc.

[0038] (6) Examples of surfactants Anionic surfactants include fatty acid soaps, α-acyl sulfonates, alkyl sulfonates, alkylallyl sulfonates, alkylnaphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkylylalkyl taurates, N-acyl amino acid salts, POE alkyl ether carboxylates, alkyl sulfosuccinates, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptides, perfluoroalkyl phosphates, and the like. Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, beheninamidepropyldimethylhydroxypropylammonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amideamine type. Nonionic surfactants include propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE-POP copolymers, POE-POP alkyl ethers, polyether-modified silicone lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of natural surfactants include lecithin, saponin, and sugar-based surfactants.

[0039] (7) Examples of polyhydric alcohols and sugars Examples include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugars, sulfated trehalose, pullulan, etc. Chemically modified versions of these can also be used.

[0040] (8) Examples of polymers Acrylic acid ester / methacrylic acid ester copolymer (Plussize, manufactured by Go-O Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Corporation), vinyl acetate / crotonic acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC Corporation), methyl vinyl ether maleate half ester (Gantlets ES, manufactured by ISP Corporation), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubymer, manufactured by BASF Corporation), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubiscol VAP, manufactured by BASF Corporation), vinyl acetate β / crotonic acid copolymer (Rubicet CA, BASF), vinyl acetate / crotonic acid / vinylpyrrolidone copolymer (Rubicet CAP, BASF), vinylpyrrolidone / acrylate copolymer (Rubiflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Examples include anionic polymer compounds such as those manufactured by Goodrich, amphoteric polymer compounds such as the amphoteric acetate of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation), octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC), cationic polymer compounds such as the quaternary compound of vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP), methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubicort, manufactured by BASF), and nonionic polymer compounds such as polyvinylpyrrolidone (Rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (Rubiscol VA, manufactured by BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer 937, manufactured by ISP), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer VC713, manufactured by ISP).Furthermore, naturally derived polymer compounds such as cellulose or its derivatives, keratin and collagen or their derivatives, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, acacia gum, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran can also be suitably used.

[0041] (9) Examples of bioactive ingredients Bioactive ingredients include substances that provide some kind of bioactivity to the skin when applied to the skin. Examples include whitening ingredients, immune stimulants, anti-aging agents, UV protection agents, slimming agents, skin tightening agents, antioxidants, hair growth agents, hair tonics, moisturizers, blood circulation promoters, antibacterial agents, disinfectants, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation relievers, analgesics, cell activators, enzyme components, etc. Examples of suitable ingredients include Angelica keiskei extract, avocado extract, Hydrangea macrophylla extract, Althaea officinalis extract, Arnica montana extract, Aloe vera extract, apricot extract, apricot kernel extract, Ginkgo biloba extract, Fennel extract, Turmeric extract, Oolong tea extract, Rosa multiflora extract, Echinacea japonica leaf extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, Barley extract, Hypericum perforatum extract, Lamium album extract, Nasturtium officinale extract, Orange extract, Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, Artemisia capillaris extract, licorice extract, carrot extract, Arctium moniliforme extract, cinchona extract, cucumber extract, guanosine, gardenia extract, bamboo grass extract, Sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, corn Rice bran fermentation extract, rice germ oil, comfrey extract, collagen, lingonberry extract, asarum extract, bupleurum extract, umbilical cord extract, salvia extract, soapwort extract, bamboo extract, hawthorn extract, sansho extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, peony extract, calamus root extract, birch extract, horsetail extract, ivy extract, hawthorn extract, ce Elderflower extract, yarrow extract, peppermint extract, sage extract, mallow extract, Cnidium officinale extract, Swertia japonica extract, soybean extract, jujube extract, thyme extract, tea extract, clove extract, cogongrass extract, citrus peel extract, angelica extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract,Examples of extracts include Ophiopogon japonicus extract, parsley extract, honey, witch hazel extract, Parietaria trifolia extract, Isodon japonicus extract, bisabolol, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, etc. Furthermore, it contains biopolymers such as deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane; moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine; oily ingredients such as sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids; ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, and guaiazulene. Immunostimulants such as hydroxyl, hydrocholtisone, vitamins such as vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, saponins, cell activators such as alpha-hydroxy acids and beta-hydroxy acids, blood circulation promoters such as gamma-oryzanol and vitamin E derivatives, Wound-healing agents such as retinol and retinol derivatives, whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione, cepharanthine, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol All, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, vanillyl nonyl acid, vanillyl nonanoate, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, gamma-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharis tincture, cyclosporine, zinc pyrithione, hydrocoltisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil,Examples of hair growth products include Sasanishiki rice extract, placenta, yuzu seed extract, blueberry extract, lingonberry extract, Cistanche extract, black rice extract, green coffee bean extract, resveratrol, kiwi seed extract, strawberry seed extract, and cherry blossom extract.

[0042] (10) Examples of antioxidants Examples include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tol biguanide, nordihydroguaiaretinic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, and plant extracts with antioxidant effects such as apple extract and clove extract.

[0043] Furthermore, this composition can also be used as a cosmetic. Examples of cosmetic forms that may incorporate this composition include lotions, soaps, facial cleansers, bath additives, creams, lotions, toners, colognes, shaving creams, shaving lotions, cosmetic oils, sunscreen lotions, face powders, foundations, perfumes, face masks, nail creams, nail polish, nail polish removers, eyebrow pencils, blushes, eye creams, eyeshadows, mascaras, eyeliners, lipsticks, lip balms, shampoos, conditioners, hair dyes, dispersions, and cleansing products.

[0044] In addition to the composition according to the present invention, the cosmetic composition in the above form may contain, to the extent that it does not impair the effects of the composition, other ingredients used in topical skin preparations such as cosmetics and quasi-drugs, such as oils, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc. Examples are listed below, but the present invention is not limited to these examples.

[0045] (1) Examples of oil contentEster-based oil phase components: Glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diethyl adipate Sopropyl, Isoarachil Neopentanoate, Caprylic / Capric Triglyceride, Trimethylolpropane Tri-2-ethylhexanoate, Trimethylolpropane Triisostearate, Pentaerythritol Tetra-2-ethylhexanoate, Cetyl Caprylate, Decyl Laurate, Hexyl Laurate, Decyl Myristate, Myristyl Myristate, Cetyl Myristate, Stearyl Stearate, Decyl Oleate, Cetyl Ricinoleate, Isostearyl Laurate, Isotridecyl Myristate, Isocetyl Myristate, Iso Tearyl, Isocetyl Palmitate, Isostearyl Palmitate, Octyl Stearate, Isocetyl Stearate, Isodecyl Oleate, Octyldodecyl Oleate, Octyldodecyl Linoleate, Isopropyl Isostearate, Cetostearyl 2-Ethylhexanoate, Stearyl 2-Ethylhexanoate, Hexyl Isostearate, Ethylene Glycol Dioctanoate, Ethylene Glycol Dioleate, Propylene Glycol Dicaprate, Di(Capryl / Capric Acid)Propylene Glycol, Propylene Glycol Dicaprylate, Dicaprin Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate,Dialkyl (C12-18) carbonate, triisocetyl citrate, triisoarachil citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate Examples include ropil, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate. Hydrocarbon-based oil phase components include squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and naturally derived waxes: animal oils and their hydrogenated oils such as beef tallow, hydrogenated beef tallow, pork tallow, hydrogenated pork tallow, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, Examples include hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, meadowfoam oil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, hydrogenated coconut oil, and other vegetable oils and their hydrogenated oils, beeswax, high-acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, montan wax, and other waxes. Silicone-based oil phase components include dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, silicone RTV rubber, etc. Examples of fluorine-based oil phase components include perfluoropolyethers, fluorine-modified organopolysiloxanes, fluorinated pitch, fluorocarbons, fluoroalcohols, and fluoroalkyl / polyoxyalkylene copolymerized organopolysiloxanes.

[0046] (2) Examples of higher alcohols Examples include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0047] (3) Examples of fatty acids Examples include caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0048] (4) Examples of UV absorbers Para-aminobenzoic acid, amyl para-aminobenzoate, ethyl dihydroxypropyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyl dimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl mono-2-ethylhexanoate dipara-methoxycinnamate, isopropyl para-methoxycinnamate, diethanolamine para-methoxyhydrocinnamate salt, diisopropyl / diisopropyl cinnamic acid ester mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, di Examples include hydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenyl acrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and their polymer derivatives and silane derivatives.

[0049] (5) Examples of powders and pigments Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake, Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polymers such as polyethylene powder, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, Prussian blue, zinc oxide, titanium dioxide, acid Examples include white pigments such as cerium oxide, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium mica, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular restrictions on the shape (spherical, rod-shaped, needle-shaped, plate-shaped, irregular shape, flake-shaped, spindle-shaped, etc.) and particle size of these powders. These powders may or may not have been pre-treated by conventional surface treatments such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil treatment, N-acylated lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc.

[0050] (6) Examples of surfactants Anionic surfactants include fatty acid soaps, α-acyl sulfonates, alkyl sulfonates, alkylallyl sulfonates, alkylnaphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkylylalkyl taurates, N-acyl amino acid salts, POE alkyl ether carboxylates, alkyl sulfosuccinates, alkyl sulfoacetate sodium, acylated hydrolyzed collagen peptides, perfluoroalkyl phosphates, and the like. Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, beheninamidepropyldimethylhydroxypropylammonium chloride, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like. Amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amidesulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amideamine type. Nonionic surfactants include propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE-POP copolymers, POE-POP alkyl ethers, polyether-modified silicone lauric acid alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like. Examples of natural surfactants include lecithin, saponin, and sugar-based surfactants.

[0051] (7) Examples of polyhydric alcohols and sugars Examples include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugars, sulfated trehalose, pullulan, etc. Chemically modified versions of these can also be used.

[0052] (8) Examples of polymers Acrylic acid ester / methacrylic acid ester copolymer (Plussize, manufactured by Go-O Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Corporation), vinyl acetate / crotonic acid / vinyl neodecanate copolymer (28-2930, manufactured by NSC Corporation), methyl vinyl ether maleate half ester (Gantlets ES, manufactured by ISP Corporation), T-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubymer, manufactured by BASF Corporation), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (Rubiscol VAP, manufactured by BASF Corporation), vinyl acetate β / crotonic acid copolymer (Rubicet CA, BASF), vinyl acetate / crotonic acid / vinylpyrrolidone copolymer (Rubicet CAP, BASF), vinylpyrrolidone / acrylate copolymer (Rubiflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulene, BF Examples include anionic polymer compounds such as those manufactured by Goodrich, amphoteric polymer compounds such as the amphoteric acetate of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation), octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC), cationic polymer compounds such as the quaternary compound of vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP), methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubicort, manufactured by BASF), and nonionic polymer compounds such as polyvinylpyrrolidone (Rubiscol K, manufactured by BASF), vinylpyrrolidone / vinyl acetate copolymer (Rubiscol VA, manufactured by BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer 937, manufactured by ISP), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer VC713, manufactured by ISP).Furthermore, naturally derived polymer compounds such as cellulose or its derivatives, keratin and collagen or their derivatives, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, acacia gum, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran can also be suitably used.

[0053] (9) Examples of bioactive ingredients Bioactive ingredients include substances that provide some kind of bioactivity to the skin when applied to the skin. Examples include whitening ingredients, immune stimulants, anti-aging agents, UV protection agents, slimming agents, skin tightening agents, antioxidants, hair growth agents, hair tonics, moisturizers, blood circulation promoters, antibacterial agents, disinfectants, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation relievers, analgesics, cell activators, enzyme components, etc. Examples of suitable ingredients include Angelica keiskei extract, avocado extract, Hydrangea macrophylla extract, Althaea officinalis extract, Arnica montana extract, Aloe vera extract, apricot extract, apricot kernel extract, Ginkgo biloba extract, Fennel extract, Turmeric extract, Oolong tea extract, Rosa multiflora extract, Echinacea japonica leaf extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, Barley extract, Hypericum perforatum extract, Lamium album extract, Nasturtium officinale extract, Orange extract, Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, Artemisia capillaris extract, licorice extract, carrot extract, Arctium moniliforme extract, cinchona extract, cucumber extract, guanosine, gardenia extract, bamboo grass extract, Sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, corn Rice bran fermentation extract, rice germ oil, comfrey extract, collagen, lingonberry extract, asarum extract, bupleurum extract, umbilical cord extract, salvia extract, soapwort extract, bamboo extract, hawthorn extract, sansho extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, peony extract, calamus root extract, birch extract, horsetail extract, ivy extract, hawthorn extract, ce Elderflower extract, yarrow extract, peppermint extract, sage extract, mallow extract, Cnidium officinale extract, Swertia japonica extract, soybean extract, jujube extract, thyme extract, tea extract, clove extract, cogongrass extract, citrus peel extract, angelica extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract,Examples of extracts include Ophiopogon japonicus extract, parsley extract, honey, witch hazel extract, Parietaria trifolia extract, Isodon japonicus extract, bisabolol, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, etc. Furthermore, it contains biopolymers such as deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane; moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine; oily ingredients such as sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids; ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, and guaiazulene. Immunostimulants such as hydroxyl, hydrocholtisone, vitamins such as vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester, active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, saponins, cell activators such as alpha-hydroxy acids and beta-hydroxy acids, blood circulation promoters such as gamma-oryzanol and vitamin E derivatives, Wound-healing agents such as retinol and retinol derivatives, whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione, cepharanthine, licorice extract, capsicum tincture, hinokitiol, garlic iodide extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropylmethylphenol, estradiol All, ethinylestradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, vanillyl nonyl acid, vanillyl nonanoate, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, gamma-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharis tincture, cyclosporine, zinc pyrithione, hydrocoltisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil,Examples of hair growth products include Sasanishiki rice extract, placenta, yuzu seed extract, blueberry extract, lingonberry extract, Cistanche extract, black rice extract, green coffee bean extract, resveratrol, kiwi seed extract, strawberry seed extract, and cherry blossom extract.

[0054] (10) Examples of antioxidants Examples include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tol biguanide, nordihydroguaiaretinic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, and plant extracts with antioxidant effects such as apple extract and clove extract.

[0055] (11) Example of Be Examples include purified water, ethanol, lower alcohols, ethers, LPG, fluorocarbons, N-methylpyrrolidone, fluoroalcohols, volatile linear silicones, and next-generation chlorofluorocarbons (CFCs). [Examples]

[0056] The following describes embodiments of the present invention. The present invention is not limited to the embodiments described below. Example: Regarding Kenyan purple tea (variety name TRFK306) extract (GHG-containing composition) In this embodiment, the Kenyan purple tea (variety name TRFK306) extract used was "Purple Tea Extract-WSPS" manufactured by Oryza Oil & Fat Chemical Co., Ltd., which is an aqueous extract of Kenyan purple tea (variety name TRFK306).

[0057] Component analysis of Kenyan purple tea (variety name TRFK306 (hereinafter simply referred to as "purple tea extract")) When the purple tea extract of this example was analyzed by HPLC under the following conditions, a peak of a component unique to purple tea extract, not found in common teas such as green tea, oolong tea, or black tea, was observed at 27.5 min (indicated by the arrow in Figure 1). Sample preparation: 350 mg of purple tea extract was dissolved in a 30% methanol aqueous solution and brought to a final volume of 20 mL in a volumetric flask. The solution was diluted 2-fold, filtered, and then analyzed by HPLC. The HPLC analysis conditions were as follows: HPLC analysis conditions Flow rate: 0.7 mL / min Mobile phase A: 0.3% TFA aqueous solution Mobile phase B: Acetonitrile Gradient: As shown in Table 1 below. Column: SunFire C18, 4.6×150 mm (Waters) or equivalent. Column temperature: 30℃ Sample injection volume: 10 μL Detection wavelength: 280 nm

[0058] [Table 1]

[0059] The above-mentioned unique component was separated, purified, and subjected to NMR analysis. The results are shown in Table 2. Based on the results in Table 2, the known component was identified as 1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose (GHG).

[0060] [Table 2]

[0061] Quantitative analysis by HPLC using GHG purified product as a standard substance revealed that GHG is present in purple tea extract. 8.1 It contained %. Using the same method as described above, purple tea extract was prepared two more times, and the GHG content in the extract was measured using the same method. The results showed that the GHG content was as follows: 7.8%、8.1%This confirmed that the purple tea extract produced by the method of this example contains approximately 1-9% GHG.

[0062] Test example: Evaluation of the Endo180 gene expression promoting effect of purple tea extract and GHG. <Method> Human dermal fibroblasts (NHDF) are placed in a 12-well plate in a 1.0 × 10⁶ size. 5 Cells were seeded at a cell / well concentration and cultured for 1 day. After culture, purple tea extract-WSPS (100% extract preparation, polyphenol content 30%) was added to the cultured cells to a final concentration of 10 or 100 μg / mL and cultured for 1 day. RNA was extracted from the cultured cells and the expression level of the Endo180 gene was evaluated by real-time RT-PCR. The results are shown in Figure 2.

[0063] Results and Effects of the Examples As shown in Figure 2, Endo180 gene expression was significantly increased by the addition of purple tea extract-WSPS (100 μg / mL). These results suggest that purple tea extract has the effect of maintaining extracellular matrix homeostasis by promoting the uptake of collagen degradation products.

[0064] The following are examples of formulations for the Endo180 gene expression promoter of the present invention, but these examples are not intended to limit the present invention. Example formulation 1: Chewing gum Sugar 53.0 wt% Gum base 20.0 Glucose 10.0 Starch syrup 16.0 Fragrance 0.5 Endo180 gene expression enhancer 0.5 100.0 wt%

[0065] Formula example 2: Gummy Reduced starch syrup 40.0 wt% Granulated sugar 20.0 Glucose 20.0 Gelatin 4.7 Wednesday 9.68 Kiwi juice 4.0 Kiwi flavor 0.6 Dye 0.02 Endo180 gene expression enhancer 1.0 100.0 wt%

[0066] Formula example 3: Candy Sugar 50.0 wt% Starch syrup 33.0 Wednesday 14.4 organic acid 2.0 Fragrance 0.2 Endo180 gene expression enhancer 0.4 100.0 wt%

[0067] Example of formulation 4: Yogurt (hard / soft) Milk 41.5 wt% Skim milk powder 5.8 Sugar 8.0 Agar 0.15 Gelatin 0.1 Lactic acid bacteria 0.005 Endo180 gene expression enhancer 0.4 fragrance trace amount water residue 100.0 wt%

[0068] Formulation example 5: Soft drink Fructose-glucose liquid sugar 30.0 wt% Emulsifier 0.5 Endo180 gene expression enhancer 0.05 Fragrance (appropriate amount) Purified water remainder 100.0 wt%

[0069] Formulation Example 6: Soft Capsules Rice germ oil 87.0wt% Emulsifier 12.0 Endo180 gene expression enhancer 1.0 100.0 wt%

[0070] Formulation example 7: Tablets Lactose 54.0wt% Crystalline cellulose 30.0 Starch decomposition product 10.0 Glycerin fatty acid ester 5.0 Endo180 gene expression enhancer 1.0 100.0 wt%

[0071] Example of formulation 8: Granule oral preparation (pharmaceutical) Endo180 gene expression enhancer 1.0 wt% Lactose 30.0 Cornstarch 60.0 Crystalline cellulose 8.0 Polyvinylpyrrolidone 1.0 100.0 wt%

[0072] Example of formulation 9: Tablet confectionery (pharmaceutical product) Sugar 76.4 wt% Glucose 19.0 Sucrose fatty acid ester 0.2 Endo180 gene expression enhancer 0.5 Purified water 3.9 100.0 wt%

[0073] Formula Example 15: Cat Food Corn 34.0 wt% Wheat flour 35.0 Meat meal 15.0 Beef tallow 8.9 Salt 1.0 Bonito extract 4.0 Endo180 gene expression enhancer 1.0 Taurine 0.1 Vitamins 0.5 Minerals 0.5 100.0 wt%

[0074] Formula Example 16: Dog Food Corn 30.0 wt% Meat (Chicken) 15.0 Defatted soybeans 10.0 Wheat flour 25.0 Bran 5.0 Endo180 gene expression enhancer 5.0 Animal fats and oils 8.9 Oligosaccharide 0.1 Vitamin 0.5 Minerals 0.5 100.0 wt%

[0075] Formula Example 17: Cosmetic Cream Squalane 20.0 wt% Beeswax 5.0 Refined jojoba oil 5.0 Glycerin 5.0 Glycerin monostearate 2.0 Polyoxyethylene (20) sorbitan Monosterate 2.0 Endo180 gene expression enhancer 2.0 Preservative (appropriate amount) Fragrance (appropriate amount) Purified water remainder 100.0 wt%

[0076] Formulation example 18: Lotion Ethanol 5.0 wt% Glycerin 2.0 1,3-Butylene glycol 2.0 Polyethylene oleyl ether 0.5 Sodium citrate 0.1 Citric acid 0.1 Endo180 gene expression enhancer 0.1 Purified water remainder 100.0 wt%

[0077] Formula Example 19: Body Gel Macadamia nut oil 2.0 wt% Octyldodecyl myristate 10.0 Methylphenylpolysiloxane 5.0 Behenyl alcohol 3.0 Stearic acid 3.0 Batyl alcohol 1.0 Glyceryl monostearate 1.0 Polyoxyethylene sorbitol tetraoleate 2.0 Hydrogenated soybean phospholipid 1.0 Ceramide 0.1 Retinyl palmitate 0.1 Preservative (appropriate amount) Centella asiatica extract 1.0 Endo180 gene expression enhancer 1.0 1,3-Butylene glycol 5.0 Purified water remainder 100.0 wt%

[0078] Formulation example 20: Emulsion Squalane 4.0 wt% Vaseline 2.5 Cetanol 2.0 Glycerin 2.0 Lipophilic glyceryl monostearate 1.0 Stearic acid 1.0 L-arginine 1.0 Endo180 gene expression enhancer 0.5 Potassium hydroxide 0.1 fragrance trace amount Purified water remainder 100.0 wt%

[0079] Formulation Example 21: Bath additive (liquid) Propylene glycol 50.0 wt% Ethanol 20.0 Sodium sulfate 5.0 Endo180 gene expression enhancer 0.5 Lanolin 0.5 Avocado oil 0.5 Dye 1.5 Fragrance 22.0 100.0 wt% [Industrial applicability]

[0080] As described above, the present invention can provide an Endo180 gene expression promoter that is safe and has few side effects.

Claims

1. An Endo180 gene expression enhancer containing a composition containing GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose) as an active ingredient.

2. An Endo180 gene expression promoter containing a composition with GHG (1,2-di-O-galloyl-4,6-O-(S)-hexahydroxydiphenoyl-b-D-glucose) derived from Kenyan purple tea (scientific name: Camellia sinensis, variety name TRFK306) as the active ingredient.

Images