Methods and systems for analyzing receptor interactions

Abstract

This invention provides methods and systems for analyzing receptor interactions to enable high-throughput mapping, validation, and prediction of receptor sequence interactions. [Solution] The method for analyzing receptor interactions receives single-cell sequencing data including single-cell sequence data, dextramer sequence data, and single-cell T cell receptor (TCR) sequence data; filters out data associated with low-quality cells from the dextramer sequence data based on the single-cell sequence data; adjusts the dextramer sequence data based on background noise measurements; filters out data from the dextramer sequence data based on the presence or absence of α-chain or β-chain based on the single-cell TCR data; and identifies the data remaining in the normalized filtered dextramer sequence data associated with reliable TCR-pMHC (peptide-major histocompatibility complex) binding phenomena.

Claims

[Claim 1] The invention described in the specification and drawings.

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