Cell Culture Evaluation System

Abstract

To solve following problems: differences in skill levels of culture operation among operators or differences in operation among culture devices cause variations in the quality of final cell products; and it is required to check whether a desired culture operation is performed as a whole culture process.SOLUTION: A cell culture evaluation system includes a measurement unit, a first storage unit, a second storage unit, an evaluation unit and an output unit, in which: the measurement unit includes a sensor that acquires evaluation target data about motion or a state of a culture tool; the first storage unit stores the evaluation target data; the second storage unit stores reference data; the evaluation unit compares the evaluation target data with the reference data so as to evaluate an execution state of a cell culture process; and the output unit outputs an evaluation result created by the evaluation unit.SELECTED DRAWING: Figure 5

Description

[Technical field]

[0001] The present invention relates to a cell culture evaluation system. [Background technology]

[0002] Cells used in regenerative medicine and cells used in the manufacture of biopharmaceuticals need to be cultured for a long period of time while maintaining a sterile condition. However, the properties and state of cells change depending on the culture conditions and the type of cell line, and sometimes cells with the desired properties cannot be obtained. In order to mass-produce cells that maintain the desired properties, it is necessary to culture them while maintaining constant culture conditions, and various cell culture devices have been developed to achieve this.

[0003] In cell manufacturing facilities, cell culture equipment is combined depending on the purpose, and when cells that are difficult to process in a culture equipment are used, a combination of manual processes is used. However, as mentioned above, even slight differences in the culture process can affect the properties and state of the cells, so in order to manufacture cell products with a consistent quality, it is essential to train skilled workers and develop automated culture equipment that can reliably perform the culture operations of skilled workers.

[0004] Attempts have been made to quantitatively evaluate the culture operations performed by operators or automatic culture devices, and culture state monitoring systems have been developed (Patent Document 1 and Non-Patent Documents 1-2). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] JP 2013-202030 A [Non-patent literature]

[0006] [Non-Patent Document 1] Hodaka Makino, Quantification of cell culture techniques using an acceleration sensor, Medical Instrumentation, 85(4), 438-442, 2015 [Non-Patent Document 2] Ryuji Kato, Development of non-destructive quality control technology using image information processing in the manufacturing process of regenerative medicine products, Journal of Bioengineering and Biotechnology, 96(3), 121-128, 2018 Summary of the Invention [Problem to be solved by the invention]

[0007] Due to the nature of cell products, it is difficult to perform destructive testing, so the quality of the final product is guaranteed by strictly following the processes described in the standard operating procedures (SOPs). However, even if the work processes are faithfully performed, the quality of the final cell product varies depending on the difference in the worker's proficiency in the culture operation and the operation of the culture equipment. The operation of the culture equipment is checked only for the items determined by each facility and each culture equipment manufacturer during regular inspections, and it is necessary to check whether the desired culture operation is being performed throughout the entire culture process. [Means for solving the problem]

[0008] As a result of intensive research into the above-mentioned problems, the present inventors have developed a system capable of evaluating the manufacturing process of a cell product.

[0009] [1] A cell culture evaluation system including a measurement unit, a first memory unit, a second memory unit, an evaluation unit, and an output unit, wherein: The measurement unit includes a sensor for acquiring data to be evaluated regarding the operation or state of the culture device during the performance of any culture process; The first storage unit stores the evaluation target data; The second storage unit stores reference data related to a reference operation or a reference state of the culture instrument when a referenced culture process is performed; the evaluation unit compares the evaluation target data stored in the first storage unit with the reference data stored in the second storage unit to evaluate an implementation state of the cell culture process; The output unit outputs the evaluation result created by the evaluation unit, The culture device includes a culture vessel, and a pipette and / or a centrifuge tube; Cell culture evaluation system. [2] The cell culture evaluation system according to Item 1, wherein the evaluation target data and the reference data are one or more selected from the group consisting of the acceleration, angular velocity, position, attitude, operating time, number of uses and temperature of the culture instrument, as well as the amount of liquid contained in the culture instrument, dissolved oxygen (DO) in the liquid, turbidity, discharge flow rate, suction flow rate and pH. [3] The evaluation unit compares the evaluation target data stored in the first storage unit with the reference data stored in the second storage unit, and when the evaluation target data at a given time point deviates from a predetermined condition set in the reference data at the corresponding time point, evaluates that an abnormality has occurred at the given time point; The output unit outputs an evaluation result including information regarding a time point when the abnormality occurred. 3. The cell culture evaluation system according to item 1 or 2. [4] The cell culture evaluation system according to any one of items 1 to 3, wherein the output unit outputs the evaluation result as a time-series trend graph of the evaluation target data and the reference data. [5] The cell culture evaluation system according to Item 4, wherein the trend graph can be enlarged and reduced to any desired magnification. [6] The cell culture evaluation system according to item 3, wherein the output unit outputs the evaluation target data and the reference data as a time-series trend graph, and displays information on the time point at which it is evaluated that the abnormality has occurred on the trend graph. [7] The cell culture evaluation system according to any one of items 1 to 6, characterized in that the measurement unit comprises a third memory unit that stores the evaluation target data, and the evaluation target data stored in the third memory unit is transferred to the first memory unit for use. [8] The cell culture evaluation system according to any one of items 1 to 7, wherein the first storage unit is a portable storage device. [9] The measurement unit includes a first transmission unit for transmitting the evaluation target data, 9. The cell culture evaluation system according to any one of items 1 to 8, wherein the first storage unit receives and stores the evaluation target data transmitted from the first transmission unit via the first reception unit.

[10] The cell culture evaluation system further comprises a sensor control unit that issues instructions to control start and stop of operation of the measuring unit, and a second transmission unit that transmits the instructions; 10. The cell culture evaluation system according to any one of items 1 to 9, wherein the measurement unit includes a second receiving unit for receiving the instruction transmitted from the second transmitting unit.

[11] The cell culture evaluation system according to any one of items 1 to 10, wherein the cell culture evaluation system comprises a third transmitting unit for transmitting parameters related to measurement conditions for the operation of a sensor provided in the measurement unit, and the measurement unit further comprises a third receiving unit for receiving the parameters transmitted from the third transmitting unit.

[12] Further comprising a photographing unit and a fourth memory unit, The photographing unit acquires an evaluation target image related to an operation or a state of the culture instrument during the arbitrary culture process; The second storage unit stores a reference image related to the reference operation or the reference state of the culture instrument when the referenced culture process is performed; The fourth storage unit stores the evaluation target image; The output unit outputs the reference image and / or the evaluation target image in conjunction with the evaluation result as necessary. 12. The cell culture evaluation system according to any one of items 1 to 11.

[13] The cell culture evaluation system described in Item 12, characterized in that the photographing unit is provided with a fifth memory unit that stores the image to be evaluated, and the image to be evaluated stored in the fifth memory unit is transferred to the fourth memory unit for use.

[14] The cell culture evaluation system described in any one of items 12 and 13, wherein the fourth memory unit is a portable memory device.

[15] The photographing unit includes a fourth transmission unit for transmitting the evaluation target image, 15. The cell culture evaluation system according to any one of items 12 to 14, wherein the fourth storage unit receives and stores the evaluation target image transmitted from the fourth transmission unit via the fourth reception unit.

[16] The cell culture evaluation system according to any one of items 1 to 15, which evaluates a culture process carried out by a human or an automatic culture device.

[17] The second memory unit stores a layout of the culture instrument and the culture material over time when the referenced culture process is performed, The output unit outputs the evaluation result linked to the layout. 16. The cell culture evaluation system according to any one of items 1 to 15. Effect of the Invention

[0010] According to the present invention, it is possible to quantitatively evaluate the actions and conditions in the cell culture process performed by an operator and / or a cell culture device, and to analyze the actions or conditions that may affect the quality of the cells. Furthermore, by using the present invention, it is possible to guarantee the quality of the final cell product. [Brief description of the drawings]

[0011] [Figure 1] FIG. 1 is a schematic configuration diagram of a cell culture evaluation system according to one embodiment. [Diagram 2] FIG. 2 is a schematic configuration diagram of a cell culture evaluation system according to one embodiment. [Diagram 3] FIG. 3 is a schematic configuration diagram of a cell culture evaluation system according to one embodiment. [Figure 4] FIG. 4 is a schematic configuration diagram of a cell culture evaluation system according to one embodiment. [Diagram 5]FIG. 5 shows an example of an image displayed on an output section of a cell culture evaluation system according to one embodiment. [Figure 6] FIG. 6 shows an example of an image displayed on an output section of a cell culture evaluation system according to one embodiment. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The present invention will be described below through embodiments of the invention, with reference to the drawings as necessary. However, the following embodiments do not limit the invention according to the claims, and the technical features described in the embodiments may be used in combination.

[0013] In this specification, terms such as "first," "second," "third," "fourth," etc. are used to distinguish one element from other elements. For example, a first element may be expressed as a second element, and similarly, a second element may be expressed as a first element, without departing from the scope of the present invention.

[0014] <Cell culture evaluation system (first aspect)> 1 shows a schematic configuration diagram of a cell culture evaluation system 1 according to one embodiment of the present invention. In one embodiment, the cell culture evaluation system 1 includes a measurement unit 10, a first storage section 20, a second storage section 21, an evaluation section 30, and an output section 40. The first storage section 20, the second storage section 21, the evaluation section 30, and the output section 40 are or can be electrically or electronically connected to each other.

[0015] The measurement unit 10 includes a sensor 100 that acquires evaluation target data related to the operation or state of the culture tool 70 during an arbitrary culture process. In this specification, the term "any culture process" refers to any process that handles cells, such as a process of culturing cells (e.g., maintenance culture, expansion culture, subculture, etc.), as well as a process of recovering, processing, or freezing cells or substances produced by cells (e.g., proteins, antibodies, viruses, etc.), and is not particularly limited. The culture process that is the evaluation target of the cell culture evaluation system 1 of the present invention may be performed by a human or may be performed by an automatic culture device (e.g., a robot 80).

[0016] The cell culture evaluation system 1 of the present invention acquires evaluation target data related to the operation or state of a culture tool 70 used during the culture process. The culture tool 70 evaluated by the cell culture evaluation system 1 of the present invention includes a culture vessel (for example, a petri dish-type, bottle-type, or any other shaped culture vessel or storage vessel, etc.), as well as a pipette and / or a centrifuge tube. Thus, although not shown, at least two measurement units 10 are provided in the cell culture evaluation system 1. The pipette used in the present invention may be any pipette used for cell culture, and is not particularly limited. The centrifuge tube used in the present invention may be any centrifuge tube that can be used in a centrifuge, and is not particularly limited.

[0017] In this specification, "evaluation target data related to the operation or state of the culture instrument" and "reference data related to the reference operation or reference state of the culture instrument" refer to data related to the operation or state of the culture instrument, which is selected from the group consisting of, for example, the acceleration, angular velocity, position, attitude, operating time, number of uses, and temperature of the culture instrument, as well as the amount of liquid contained in the culture instrument, the dissolved oxygen (DO) in the liquid, turbidity, discharge flow rate, suction flow rate, and pH. That is, it is quantifiable data indicating the operation or state of the culture instrument that may affect the state of the cells. Therefore, the sensor 100 in the measurement unit 10 may be a sensor capable of acquiring the above-mentioned evaluation target data, and may be, for example, an acceleration sensor, a gyro sensor, a geomagnetic sensor, an impact sensor, a magnetic position sensor, a GPS sensor, a pH sensor, an optical sensor, an ultrasonic sensor, a pressure sensor, a flow rate sensor, a weight sensor, a level sensor, a proximity sensor, a temperature sensor, or other known sensors may be used alone or in combination. In the present invention, the measurement unit 10 may be, for example, a measurement unit described in JP 2013-202030 A.

[0018] The first storage unit 20 can store the evaluation target data acquired by the sensor 100. The first storage unit 20 may be a memory device such as a RAM, a ROM, or a flash memory, a fixed disk device such as a hard disk drive, or a portable storage medium such as a flexible disk or an optical disk, or may be a cloud server connected by electrical communication via the Internet. For example, as illustrated in FIG. 1, when the first storage unit 20 is a portable storage device, the evaluation target data acquired by the sensor 100 is stored in the first storage unit 20, and the first storage unit 20 is connected to a computing unit equipped with an evaluation unit 30 via, for example, a connection slot, and can be used for evaluation.

[0019] The second storage unit 21 stores reference data relating to a reference operation or a reference state of the culture instrument when the referenced culture step is performed. The reference data refers to data relating to the operation or state of the culture instrument acquired when the culture step performed to manufacture a final cell product having a desired quality is performed, and includes data when a final cell product having a desired quality is obtained and when it is not obtained. Therefore, the reference data may be data acquired by the measurement unit 10 of the same cell culture evaluation system 1, or may be data acquired by the measurement unit 10 of another cell culture evaluation system 1. The second storage unit 21 (and the third storage unit, fourth storage unit, fifth storage unit, and sixth storage unit described later) may be a memory device such as a RAM, a ROM, or a flash memory, a fixed disk device such as a hard disk drive, or a portable storage medium such as a flexible disk or an optical disk, or may be a cloud server electrically connected via the Internet, like the first storage unit 20.

[0020] The evaluation unit 30 compares the evaluation target data stored in the first storage unit 20 with the reference data stored in the second storage unit 21 to evaluate the implementation state of the cell culture process. The evaluation of the culture process by the evaluation unit 30 is performed by a processor included in the evaluation unit, such as a CPU or a GPU. For example, the evaluation unit 30 reads and compares the time-series evaluation target data stored in the first storage unit 20 with the time-series reference data stored in the second storage unit 21, and when the evaluation target data at an arbitrary time deviates from a predetermined condition set in the reference data at the corresponding time, it can evaluate that an abnormality has occurred at that arbitrary time (see FIG. 5(B)). In addition, the evaluation unit 30 reads and compares the time-series evaluation target data stored in the first storage unit 20 with the time-series reference data stored in the second storage unit 21, and when the evaluation target data at an arbitrary time deviates from a predetermined condition set in the reference data at the corresponding time, for example, in the cell culture process, it can make an evaluation to improve that point and propose an improvement point.

[0021] The output unit 40 is configured to output the evaluation result created by the evaluation unit 30. The output unit 40 may be, for example, a monitor or a printer, but is preferably a monitor. In one embodiment, the output unit 40 is configured to output the evaluation result including information on the time point at which the abnormality occurred. In another embodiment, the output unit 40 may be configured to output the evaluation result as a time-series trend graph of the evaluation target data and the reference data (see, for example, FIGS. 5 and 6). The trend graph may be enlarged and reduced at any magnification, which makes it possible to grasp information on the time point at which the abnormality was determined in detail. In another embodiment, the output unit 40 may output the evaluation target data and the reference data as a time-series trend graph, and display information on the time point at which the abnormality was evaluated to have occurred on the trend graph (see, for example, FIGS. 5 and 6). This makes it easier to visually confirm information on the time point at which the abnormality was evaluated in the cell culture evaluation system 1.

[0022] <Cell culture evaluation system (second aspect)> FIG. 2 shows a schematic diagram of a cell culture evaluation system 1a according to one embodiment of the present invention. The above description of the corresponding members in the first embodiment applies to the description of each member in the cell culture evaluation system 1a of the second embodiment, which is given the same reference numerals as those of the members provided in the cell culture evaluation system 1 of the first embodiment, except that "a" is added to the reference numerals of each member. Here, only members to which the description of each member in the first embodiment does not apply will be described. Note that the culture instrument 70 and the robot 80 shown in FIG. 1 are omitted in FIGS. 2 to 4.

[0023] In one embodiment, the measurement unit 10a of the cell culture evaluation system 1a includes a first transmission unit 50a for transmitting the evaluation target data. The cell culture evaluation system 1a also includes a first reception unit 51a electrically connected to the first storage unit 20a. This allows the first storage unit 20a to receive and store the evaluation target data transmitted from the first transmission unit 50a via the first reception unit 51a. The communication between the first transmission unit 50a and the first reception unit 51a may be wired communication, wireless communication, or communication via a portable storage medium.

[0024] The first storage unit 20a and the second storage unit 21a may be separate storage media, or may be separate areas of the same storage medium.

[0025] <Cell culture evaluation system (third aspect)> 3 shows a schematic diagram of a cell culture evaluation system 1b according to one embodiment of the present invention. The above-mentioned explanations of the corresponding members in the first and second embodiments are applied to the explanations of the members of the cell culture evaluation system 1b of the third embodiment, which are given the same reference numbers as the members provided in the cell culture evaluation system 1 of the first embodiment and the cell culture evaluation system 1a of the second embodiment, except that "b" is added to the reference numbers of the members. Here, only the members to which the explanations of the members in the first and second embodiments do not apply are explained.

[0026] In one embodiment, the measurement unit 10b in the cell culture evaluation system 1b may include a third storage unit 22b that stores the evaluation target data. Thus, in the cell culture evaluation system 1b, the evaluation target data stored in the third storage unit 22b can be transferred to the first storage unit 20b for use.

[0027] In one embodiment, the evaluation target data temporarily stored in the third memory unit 22b may be transmitted from the first transmitting unit 50b provided in the measurement unit 10b, received by the first receiving unit 51b, and transferred to and stored in the first memory unit 20b.

[0028] In one embodiment, the cell culture evaluation system 1b includes a sensor control unit 31b that issues instructions to control the start and stop of the operation of the measurement unit 10b, and a second transmission unit 52b for transmitting the instructions, and the measurement unit 10b may further include a second reception unit 53b for receiving the instructions transmitted from the second transmission unit 52b. This allows the sensor 100b of the measurement unit 10b to start and stop operation at any time.

[0029] In one embodiment, the cell culture evaluation system 1b includes a third transmitting unit 54b for transmitting parameters related to the measurement conditions of the operation of the sensor 100b included in the measuring unit 10b, and the measuring unit 10b may further include a third receiving unit 55b for receiving the parameters transmitted from the third transmitting unit 54b. This makes it possible to set at least one of the measurement range, period, and measurement interval of the evaluation target data that can be acquired by the sensor 100b.

[0030] <Cell Culture Evaluation System (Fourth Aspect)> 4 shows a schematic configuration diagram of a cell culture evaluation system 1c according to one embodiment of the present invention. The above-mentioned explanations of the corresponding members in the first to third embodiments are applied to the explanations of the members of the cell culture evaluation system 1c of the fourth embodiment, which are given the same reference numbers as the members provided in the cell culture evaluation systems (1, 1a, and 1b) of the first to third embodiments, except that "c" is added to the reference numbers of the members. Here, only the members to which the explanations of the members in the first to third embodiments do not apply are explained.

[0031] In one embodiment, the cell culture evaluation system 1c may include a photographing unit 60c and a fourth storage unit 23c. In this case, the photographing unit 60c acquires an evaluation target image related to the operation or state of the culture instrument 70 during the implementation of an arbitrary culture step; the second storage unit 21c stores a reference image related to a reference operation or the reference state of the culture instrument 70 during the implementation of the reference culture step; the fourth storage unit 23c stores the evaluation target image; and the output unit 40c outputs the reference image and / or the evaluation target image in conjunction with the evaluation result as necessary (see, for example, FIG. 6). The photographing unit 60c includes a photographing means 600c. The photographing means 600c may be, for example, a camera capable of photographing still images or a video camera capable of photographing moving images, but is preferably a video camera. If the photographing means 600c is a video camera, it becomes possible to visually confirm in more detail the abnormality of the operation being performed in the culture step at the time when it is determined to be abnormal by the cell culture evaluation system 1c.

[0032] In one embodiment, the photographing unit 60c may include a fifth storage unit 24c that stores the evaluation target image. In this way, in the cell culture evaluation system 1c, the evaluation target image stored in the fifth storage unit 24c can be transferred to the fourth storage unit 23c for use.

[0033] In one embodiment, the evaluation target image temporarily stored in the fifth memory unit 24c may be transmitted from a fourth transmitting unit 56c provided in the photographing unit 60c, received by a fourth receiving unit 57c, and transferred to and stored in the fourth memory unit 23c.

[0034] In one embodiment, the photographing unit 60c does not include the fifth memory unit 24c, and the image to be evaluated is transmitted from the fourth transmitting unit 56c, received by the fourth receiving unit 57c, and transferred to and stored in the fourth memory unit 23c.

[0035] In one embodiment, the cell culture evaluation system 1c includes an imaging means control unit 32c that issues instructions to control the start and stop of the operation of the imaging unit 60c, and a fifth transmission unit 58c for transmitting the instructions, and the imaging unit 60c may further include a fifth reception unit 59c for receiving the instructions transmitted from the fifth transmission unit 58c. This allows the imaging means 600c of the imaging unit 60c to be started and stopped at any time.

[0036] In one embodiment, the fourth storage unit 23c may be a portable storage device. In this case, the evaluation target image acquired by the photographing means 600c of the photographing unit 60c is stored in the fourth storage unit 23c, and the fourth storage unit 23c can be connected to a computing device equipped with the evaluation unit 30c via, for example, a connection slot, and used for evaluation (not shown).

[0037] In one embodiment, the second storage unit 21c in the cell culture evaluation system 1c may store the layout of the culture tools 70 and culture materials (e.g., medium bottles, pipettes, culture vessels, centrifuge tubes, etc.) over time when the referenced culture process is performed (see, for example, FIG. 5(D)). In this case, the output unit 40c can output the evaluation results linked to the layout (FIGS. 5 and 6).

[0038] In this specification, the transmitting units (1st to 5th transmitting units) and the receiving units (1st to 5th receiving units) are described as separate elements for the sake of convenience of explanation, but it should be noted that these may be a bidirectional "communication unit" in which the transmitting unit and the receiving unit are integrated, or may be the same transmitting unit or receiving unit. [Explanation of symbols]

[0039] 1, 1a, 1b, 1c Cell Culture Evaluation System 10, 10a, 10b, 10c Measuring units 100, 100a, 100b, 100c Sensor 20, 20a First memory unit 21, 21a 2nd memory section 22b, 22c 3rd memory section 23c 4th memory section 24c 5th memory section 30, 30a, 30b, 30c Evaluation section 31b, 31c Sensor control unit 32c Photographing means control section 40, 40a, 40b, 40c Output section 50a, 50b, 50c First transmitter 51a, 51b, 51c First receiving unit 52b, 52c Second transmission unit 53b, 53c Second receiving unit 54b, 54c Third transmission unit 55b, 55c Third receiving section 56c 4th Transmitter 57c 4th Receiving Section 58c 5th Transmission Unit 59c 5th Receiving Section 60c photography unit 600c shooting method 70 Culture equipment 80 Robot

Claims

1. A cell culture evaluation system for evaluating a culture process performed by a human manual operation (excluding an operation via a robot) or a human manual operation (excluding an operation via a robot) and an automatic culture device, the system including a measurement unit, a first memory unit, a second memory unit, an evaluation unit, and an output unit, wherein: The measurement unit includes a sensor for acquiring data to be evaluated regarding the operation or state of the culture tool during the execution of any culture process performed by a human manual operation (but excluding the operation via a robot) or by a human manual operation (but excluding the operation via a robot) and an automatic culture device; The first storage unit stores the evaluation target data; The second storage unit stores reference data regarding a reference operation or a reference state of the culture instrument during a culture process performed by a human manual operation (excluding a robotic operation) or a human manual operation (excluding a robotic operation) and an automatic culture device; the evaluation unit compares the evaluation target data stored in the first storage unit with the reference data stored in the second storage unit to evaluate an implementation state of the cell culture process; The output unit outputs the evaluation result created by the evaluation unit, The culture device includes a culture vessel, and a pipette and / or a centrifuge tube; The evaluation target data and the reference data are one or more selected from the group consisting of an acceleration of the culture instrument, an angular velocity of the culture instrument, a position of the culture instrument, an attitude of the culture instrument, an operating time of the culture instrument, a number of times the culture instrument has been used, a temperature of the culture instrument, an amount of liquid contained in the culture instrument, dissolved oxygen (DO) in the liquid contained in the culture instrument, a turbidity of the liquid contained in the culture instrument, a discharge flow rate of the liquid contained in the culture instrument, a suction flow rate of the liquid contained in the culture instrument, and a pH of the liquid contained in the culture instrument. Cell culture evaluation system.

2. the evaluation unit compares the evaluation target data stored in the first storage unit with the reference data stored in the second storage unit, and when the evaluation target data at an arbitrary time point deviates from a predetermined condition set in the reference data at the corresponding time point, evaluates that an abnormality has occurred at the arbitrary time point; The output unit outputs an evaluation result including information regarding a time point when the abnormality occurred. The cell culture evaluation system according to claim 1 .

3. 3. The cell culture evaluation system according to claim 1, wherein the output unit outputs the evaluation result as a time-series trend graph of the evaluation target data and the reference data.

4. The cell culture evaluation system according to claim 3 , wherein the trend graph can be enlarged or reduced to an arbitrary magnification.

5. 3. The cell culture evaluation system according to claim 2, wherein the output unit outputs the evaluation target data and the reference data as a time-series trend graph, and displays information regarding a time point at which it was evaluated that the abnormality occurred on the trend graph.

6. The cell culture evaluation system according to any one of claims 1 to 5, characterized in that the measurement unit comprises a third storage unit that stores the evaluation target data, and the evaluation target data stored in the third storage unit is transferred to the first storage unit for use.

7. The cell culture evaluation system according to any one of claims 1 to 6, wherein the first storage unit is a portable storage device.

8. The measurement unit includes a first transmission unit for transmitting the evaluation target data, The cell culture evaluation system according to any one of claims 1 to 7, wherein the first storage unit receives and stores the evaluation target data transmitted from the first transmission unit via the first reception unit.

9. The cell culture evaluation system includes a sensor control unit that issues instructions for controlling start and stop of operation of the measuring unit, and a second transmission unit that transmits the instructions, The cell culture evaluation system according to any one of claims 1 to 8, wherein the measurement unit includes a second receiving unit for receiving the instruction transmitted from the second transmitting unit.

10. The cell culture evaluation system according to any one of claims 1 to 9, further comprising a third transmitting unit for transmitting parameters related to measurement conditions for the operation of a sensor provided in the measuring unit, and the measuring unit further comprising a third receiving unit for receiving the parameters transmitted from the third transmitting unit.

11. A photographing unit and a fourth storage unit, The photographing unit acquires an evaluation target image related to an operation or state of the culture instrument during the arbitrary culture process; The second storage unit stores a reference image related to the reference operation or the reference state of the culture instrument during the referenced culture process; the fourth storage unit stores the evaluation target image; The output unit outputs the reference image and / or the evaluation target image in conjunction with the evaluation result as necessary. The cell culture evaluation system according to any one of claims 1 to 10.

12. 12. The cell culture evaluation system according to claim 11, wherein the photographing unit comprises a fifth storage unit that stores the image to be evaluated, and the image to be evaluated stored in the fifth storage unit is transferred to the fourth storage unit for use.

13. The cell culture evaluation system according to claim 11 , wherein the fourth storage unit is a portable storage device.

14. the photographing unit includes a fourth transmission unit for transmitting the evaluation target image, The cell culture evaluation system according to any one of claims 11 to 13, wherein the fourth storage unit receives and stores the evaluation target image transmitted from the fourth transmission unit via the fourth reception unit.

15. The second storage unit stores a layout of the culture instrument and the culture material over time when the culture process is performed, The output unit outputs the evaluation result linked to the layout. The cell culture evaluation system according to any one of claims 1 to 14.

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