Methods in bioprocessing purification systems

Abstract

The present invention relates to a method for monitoring operational conditions in a column capture chromatography system configured for cyclical repetitive purification performed on a predetermined volume of sample feed containing a product captured in the column during loading. The method includes steps of: performing a purification cycle (51); measuring a parameter during the purification cycle (52) during sample feed loading that is indicative of breakthrough of the captured product after the column; reducing the amount of sample feed loaded during loading in the next purification cycle (56a) when breakthrough is detected; and repeating the steps above to perform another purification cycle. Each purification cycle includes loading a predetermined amount of sample feed onto the column, washing the column, and eluting at least one captured product.

Description

[Technical field]

[0001] The present invention relates to a method for monitoring and controlling a bioprocess purification system for cyclical repetitive purification of at least one product in a bioreactor system. [Background technology]

[0002] The quality of the material produced in the bioreactor (in the cell culture system) is important to achieve a reliable, robust and economical manufacturing procedure when purifying the harvest from the bioreactor.

[0003] Currently, the clarified or clean feed from the bioreactor is introduced into a column capture chromatography system configured for a cyclic purification process to extract the product. The cyclic process involves loading the feed onto a column, washing the column, eluting the product, and then cleaning the column before it is repeatedly loaded with new feed. For a given volume of feed, the purification process using a small volume column will take longer than when using a large volume column.

[0004] Therefore, in purifications using column capture chromatography systems for a given volume of feed, there is a need to modify the purification process to achieve a more efficient cyclic process, especially for small volume columns. Summary of the Invention [Problem to be solved by the invention]

[0005] It is an object of the present disclosure to provide methods, devices configured to perform the methods, and computer programs that seek to mitigate, alleviate or eliminate one or more of the above-identified deficiencies and disadvantages in the art, either singly or in any combination. [Means for solving the problem]

[0006] This object is achieved by the method as defined by the independent claims.

[0007] The advantage is that the time to perform product purification can be reduced while maintaining high yields.

[0008] Another advantage is that the purification process can be adapted to more efficiently use the volume available within the chromatography system.

[0009] Further objects and advantages can be obtained by those skilled in the art from the detailed description. [Brief description of the drawings]

[0010] [Figure 1] FIG. 1 illustrates an overview of a bioprocess purification system designed to purify products from the harvest fluid from a bioreactor. [Diagram 2] FIG. 1 illustrates the concept of controlling upstream / downstream processes within a bioprocessing purification system. [Figure 3a] FIG. 1 illustrates an idealized cyclic capture chromatography process configured to deliver product from a sample feed using only one column. [Figure 3b] FIG. 1 illustrates a cyclic capture chromatography process that uses several columns in sequence. [Figure 4] 1 is a graph illustrating pressure behavior during a cyclic capture chromatography process. [Diagram 5]FIG. 1 illustrates a first embodiment of a process for monitoring operating conditions in a single-column capture chromatography system. [Figure 6] FIG. 1 illustrates a second embodiment of a process for monitoring operating conditions in a single-column capture chromatography system. [Figure 7] FIG. 1 illustrates a third embodiment of a process for monitoring operating conditions in a single-column capture chromatography system. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] Bioprocess purification systems are designed for the production and purification of products (e.g., proteins, biomolecules from cell culture / fermentation, natural extracts, etc.) by growing cells capable of expressing the product in a cell culture bioreactor, followed by a downstream purification process (also referred to as a downstream process) to purify the product. The downstream purification process can be any suitable process capable of providing a purified product, and the process can include one or more steps. One commonly used step in the downstream purification process is chromatography. In particular, the present invention relates to a bioprocess purification system that is arranged to produce and provide a purified product from a given volume of sample feed over an extended period of time. The product is provided as a batch having a volume larger than the volume of the column, or the product is harvested from the bioreactor and purified by a downstream purification process while the cell culture is maintained. This type of cell culture is referred to herein as a "continuous cell culture process", and examples of such cell culture include perfusion cell culture and chemostat cell culture.

[0012] 1 shows an overview of a bioprocessing purification system according to one embodiment, which is configured to purify a product using a separation process. The bioprocessing purification system includes multiple steps related to cell culture 11, retention 12, capture 13, viral inactivation 14, polish 15, and delivery 16.

[0013] In one of the disclosed embodiments of the present invention, the cell culture step 11 can be a continuous cell culture process, which includes continuous addition of nutrients and continuous removal (harvesting) of products and waste products over an extended period of time. The process can be operated in perfusion, for example, by using an Alternate Tangential Filtration (ATF) device to retain the cells in the bioreactor. Alternatively, the bioreactor is operated without cell retention (i.e., a chemostat). The cell culture step includes process control for Viable Cell Density (VCD) and can also include process control for nutrients and metabolites. VCD, productivity, and product quality can be controlled by adapting the components of the cell culture medium fed to the culture or by adding certain components directly to the culture, as described in more detail below.

[0014] In some embodiments, the harvest containing the product may be clarified, for example by filtration, centrifugation, or another technique, prior to sending the harvest to a downstream purification process.

[0015] The holding step 12 is an optional step depending on the needs of the process (e.g., if a filter is in-line before the capture step 13). The step can include process control over weight, where the next step in the process begins after a pre-determined volume value is reached, or alternatively, after a certain period of time or when a pre-determined mass is reached. The holding step can be used to collect a predetermined volume of filtered feed from the perfusion cell culture.

[0016] In another embodiment of the invention, the cell culture step is omitted and a batch of sample feed containing the product is provided in the holding step 12 and provided to the purification process.

[0017] In the disclosed embodiment, the downstream purification process includes three steps: capture 13, viral inactivation 14, and polish 15. The capture step 13 can include a chromatography process in a single chromatography column. A filter can be provided in-line prior to the capture step. The capture step includes multiple batch elutions, and process control (e.g., using an in-line UV sensor) handles variations in feed concentration and resin volume. The next step begins when a pre-determined quantity value (e.g., volume, mass, or time) is reached.

[0018] In viral inactivation step 14, different options for viral inactivation are available depending on the process needs. One option is to use a batch mode with low pH for 30-60 minutes in a hold-up tank. The step can include process controls for volume, time, temperature, and pH. The next step begins when a pre-determined time is reached.

[0019] The polish step 15 can be straight-through processing (STP) with a connected batch step, or continuous chromatography with a continuous load step, or a combination thereof. The flow rate is adjusted to the perfusion rate required by the producer cells, meaning that the flow rate is determined by the preceding step. The steps can include process controls on UV, flow, and volume, and the next step starts when a pre-determined volume and amount is reached, or alternatively when a timeout is reached.

[0020] The delivery step 16 may include a virus removal step (e.g., a virus filter) prior to the ultrafiltration step. The delivery step may be used as a concentration step for batch addition of processed harvest from the polishing step. The delivery step 16 may include continuous or batch delivery of product and may include continuous or batch removal of waste. The steps may include process control for pH, conductivity, absorbance, volume, and pressure, and delivery is realized when a pre-determined product concentration in a pre-defined environment is reached.

[0021] An automation layer 17 is used to handle decision points for the next step in the process. Different types of sensors (not shown), both in-line and off-line sensors, are integrated into the process flow to monitor different parameters that can be used to provide data to the automation layer 17 that can be used to handle the decision points. Sensors include, but are not limited to, measuring flow, VCD, weight, pressure, UV, volume, pH, conductivity, absorbance, etc.

[0022] It should be noted that UV absorption is an example of a parameter that may be monitored to detect the composition of the harvest being refined, but other parameters operating in other frequency ranges (e.g., IR, fluorescence, X-ray, etc.) may also be used.

[0023] The product quality of products produced in bioprocessing purification systems can be improved by obtaining information related to the product during the process run or the produced product itself. Attributes related to product quality must be measured and different analytical methods can be used (e.g., mass spectrometry, MS, light scattering, size exclusion chromatography, SEC, Raman spectroscopy, etc.).

[0024] The cell culture system includes a bioreactor that produces a product-containing harvest, and the cell culture process can be controlled to optimize the product quality of the product. Examples of parameters that can be controlled in the bioreactor are temperature, aeration, agitation, etc.

[0025] Figure 2 illustrates the concept of controlling upstream / downstream processes in a bioprocessing purification system. The illustration of the bioprocessing purification system is simplified and includes three steps: sample feed 20, separation 21, and batching 22. The output (exemplified in this example by an "active pharmaceutical ingredient (API)") is delivered after the batching step.

[0026] The sample feed 20 can be a cell culture step in a continuous cell culture process as described above, which includes, for example, continuous harvesting of products and waste products, or continuous addition of nutrients to a cell perfusion process with batches of sample feed provided in a volume larger than the capacity of a single column chromatography system. The sample feed including products and waste products is considered to be a harvest that is fed into a separation step 21, which can include one or more steps of a downstream purification process. The separation step includes a process for at least partially separating the products from the waste products in the harvest, and the products are transferred to a final step batching 22, where the products are handled to prepare them for delivery as an API.

[0027] After the separation step, using a mass spectrometer, MS, or spectroscopy, certain parameters or quality attributes can be measured (e.g., the composition of impurities in the product, or the amount of product fragments or aggregates). This information can be used to control 23 the upstream process. For example, if after separation, a high amount of degraded product is detected, this can be countered by changing parameters in the cell culture step, for example by increasing the flow rate of the medium into the bioreactor, to prevent degradation of the product molecules before they are introduced into the separation step 21. Alternatively, the feeding of nutrients or process parameters in the cell culture can be adjusted based on the measured quality attributes, as explained in more detail below. Variations in the composition of the sample feed provided to the separation step 21 can be detected after the column, for example, if a breakthrough of the captured product is detected (which can be countered by changing the amount of sample feed loaded on the column).

[0028] The same concept can be used to control downstream processes 24. The concentration of the product in the harvest being fed into the separation step 21 can be determined by measuring the time to load each column and the peak amount of product after elution. This information can be used to adjust the elution based on the concentration of the product in the harvest being fed into the separation step.

[0029] FIG. 3a illustrates an ideal cyclic capture chromatography process, which is configured to deliver at least one product from a given volume of sample feed using only one column with a cyclically repeated purification process. In this example, the same column is used for 200 cycles, with each purification cycle including loading a given amount of sample feed onto the column, washing the column, and eluting at least one captured product. However, this is not true, since the performance of the system deteriorates as a function of the cycles performed, and when the performance is reduced, the column needs to be cleaned using cleaning-in-place (CIP), which improves the performance of the chromatography column. And when the performance of the column is not improved by CIP, the column needs to be replaced by a new column. This process is illustrated in FIG. 3b.

[0030] Figure 3b illustrates a cyclic capture chromatography process using several columns in sequence. A CIP trigger is shown, which results in a CIP being performed on the columns. The CIP trigger can be either: - The pressure during sample feed loading increases by 10% - If the elution peak width during elution increases by, for example, >5% - Increased elution peak asymmetry

[0031] CIP will improve the performance of the system to a certain level before the column must be replaced. Below are some examples of indications that can be used to determine when a column needs to be replaced: - if the pressure during loading of the sample feed increases beyond a safe limit (as indicated by dashed line 40 in Figure 4) - When the sample feed volume drops below 70% of the dynamic binding capacity (DBC) of the resin in the column - An increase in elution peak width or elution peak asymmetry that does not result in improvement within 3 cycles after CIP is initiated

[0032] In Figure 3b, CIP is performed in response to a CIP trigger and the first column "Unit 1" is replaced by a new column "Unit 2" when a significant drop in DBC is detected. Moreover, some CIP is performed on Unit 2, but a significant increase in pressure is detected and "Unit 2" is replaced by "Unit 3". The final amount of product is produced using Unit 3.

[0033] FIG. 4 is a graph illustrating pressure curves 41 for columns used in sequence, designated "Unit 1," "Unit 2," and "Unit 3." After X cycles (e.g., 70 cycles), a significant drop in DBC is detected and the columns are replaced. The pressure curve 41 is still below the dash-dotted line 40 (indicating a safety limit for pressure) at this point. However, for Y more cycles (e.g., an additional 65 cycles), the pressure increases beyond the safety limit as purification begins, and "Unit 2" is replaced by "Unit 3" after X+Y cycles (in this example, 135 cycles). After Z additional cycles (in this example, 65 cycles), the process is completed and the final amount of product is produced after 200 cycles.

[0034] The shape of the pressure curve can be used to identify trends and thereby more effectively detect degraded performance of the column.

[0035] FIG. 5 illustrates a first embodiment of a method 50 for monitoring operating conditions in a column capture chromatography system configured for cyclical repetitive purification performed on a sample feed of a predetermined volume comprising at least one product (e.g., a target product) configured to be captured in the column during loading. The sample feed volume comes from the same feed (e.g., a batch or continuous feed). The capture chromatography system includes one single column or, alternatively, several columns coupled in parallel to which a bioreactor may be connected, the bioreactor configured to provide a sample feed of a predetermined volume to the column, the sample feed volume being larger than the volumetric capacity of the capture chromatography system. Thus, a repetitive cyclic purification is required. The process includes several steps: a) performing a purification cycle 51, which includes loading a predetermined amount of sample feed onto a column, washing the column, and eluting the captured product; b) measuring 52 at least one parameter during the purification cycle indicative of breakthrough of at least one captured product down the column during loading of the sample feed; c) as illustrated by 56, if breakthrough of at least one captured product is detected, reducing the amount of sample feed loaded during loading in the next purification cycle 56a; d) repeating steps a) through c) until a desired amount of product is produced. This is illustrated in relation to Figures 3a and 3b above.

[0036] According to some embodiments, loading of the sample at 51 is performed for a loading time (Δt); the loading time is reduced at 56a to reduce the amount of sample feed loaded and prevent breakthrough during the next purification cycle. According to some embodiments, the loading time is reduced by at least 5%. In some embodiments, the loading time is reduced by 10% or less when breakthrough is detected.

[0037] According to some embodiments, when the amount of sample feed is maintained in step c) for a predefined number of consecutive purification cycles (x), as illustrated by 58, the loading time is increased 58a to increase the amount of sample feed loaded during the next purification cycle. An example x is at least 25 cycles. According to some embodiments, the loading time is increased by 1% to find the optimal loading without risking yield loss.

[0038] According to some embodiments, the at least one parameter measured at 52 is the signal from a UV detector mounted after the column.

[0039] According to some embodiments, the method further comprises monitoring at least one additional parameter measured during elution of the at least one captured product, as illustrated by 54. The at least one additional parameter is indicative of the purification capacity of the column, and the method further comprises increasing the amount of sample feed loaded during loading of the next purification cycle, as illustrated by 58a, if the purification capacity is decreasing without an indication of breakthrough, as illustrated by 57. According to some embodiments, the amount of sample feed is increased by 1%.

[0040] According to some embodiments, the method further comprises, prior to 56, a step 54 of evaluating at least one additional parameter measured during elution of the captured product indicative of a purification capacity in the chromatographic system; and a step 55a of cleaning the column, e.g., using CIP, if the purification capacity falls below an upper predefined threshold, as indicated by 55. For example, the upper threshold corresponds to a level at which the at least one additional parameter indicates a 5-10% drop in purification capacity.

[0041] CIP will improve the performance of the system to a certain level before the column must be replaced. According to some embodiments, the column performance is monitored in step 53 and if poor performance is detected, the column needs to be replaced.

[0042] As mentioned above, below are some examples of indications that can be used to determine when a column needs to be replaced. - if the pressure during loading of the sample feed increases beyond a safe limit (as indicated by dashed line 40 in Figure 4) - When the amount of sample feed drops below 70% of the dynamic binding capacity (DBC) of the resin in the column - An increase in elution peak width or elution peak asymmetry that does not result in improvement within 3 cycles after CIP is initiated

[0043] According to some embodiments, the evaluation in step 54 is a trend analysis of the at least one additional measured parameter over time.

[0044] According to some embodiments, an additional parameter in step 54 is the elution peak area measured upon elution of the at least one captured product in relation to the amount of sample feed loaded onto the column.

[0045] According to some embodiments, the step of evaluating the at least one additional parameter further comprises replacing the column with a new column 59 if the purification capacity falls below a lower predefined threshold, as indicated by 55b, during the breakthrough detection. For example, the lower threshold corresponds to a level at which the at least one additional parameter indicates a 10-20% drop in purification capacity.

[0046] The present invention also relates to a capture chromatography system, comprising a column configured for purification performed on a sample feed comprising at least one product configured to be captured in the column during loading, and a control unit configured to control the purification in the column, the control unit being further configured to perform the method described in relation to FIG. 5.

[0047] In FIG. 6, a method 60 according to a second aspect of the present invention is disclosed, the method comprising: a) performing a purification cycle 61, comprising loading a predetermined amount of sample feed onto a column, washing the column, and eluting at least one captured product; b) measuring 62 at least one parameter during the purification cycle during loading of the sample feed that is indicative of the breakthrough of the at least one captured product after the column; and measuring 64 an additional parameter during the purification cycle during elution that is indicative of the amount of the at least one captured product after the column; c) if the amount of at least one captured product is declining without an indication of breakthrough, as shown at 67, increasing the amount of sample feed loaded during loading in the next purification cycle 68a; d) Repeating steps a) to c). It is possible to include:

[0048] According to some embodiments, the amount of sample feed in step c) is increased by 1%.

[0049] According to some embodiments, the method further comprises, prior to step c), a step 65 of evaluating at least one additional parameter; and a step 65a of cleaning the column if the purification capacity is below an upper predefined threshold.

[0050] According to some embodiments, the evaluation is a trend analysis of the at least one additional measured parameter over time.

[0051] According to some embodiments, the additional parameter is the elution peak area measured upon elution of at least one captured product relative to the amount of sample feed loaded onto the column.

[0052] According to some embodiments, the step of evaluating at least one additional parameter further comprises a step 69 of replacing the column with a new column if the purification capacity falls below the above-mentioned lower predetermined threshold while breakthrough is detected, as indicated by 65b.

[0053] According to some embodiments, step c) further comprises a step 66a of reducing the amount of sample feed loaded during loading in the next purification cycle if breakthrough of at least one captured product is detected, as indicated by 66. According to some embodiments, the amount of sample feed is reduced by at least 5%. In some embodiments, the amount of sample feed is reduced by 10% or less when breakthrough is detected.

[0054] According to some embodiments, the loading of the sample in step a) is performed during a loading time Δt; the loading time is reduced in step c) to prevent breakthrough during the next purification cycle.

[0055] According to some embodiments, as indicated by 68, when the loading time has been maintained in step c) for at least "x" consecutive purification cycles (e.g., 25 consecutive cycles), the loading time is increased for the next purification cycle 68a.

[0056] According to some embodiments, the parameter measured in step b) is the signal from a UV detector mounted after the column.

[0057] According to some embodiments, cyclical repetitive purification is performed on batches of sample feed or on a sample feed provided continuously from a cell culture reactor.

[0058] The present invention also relates to a capture chromatography system, comprising a column configured for purification performed on a sample feed including at least one product configured to be captured in the column during loading, and a control unit configured to control the purification in the column, the control unit being further configured to perform the method described in relation to FIG. 6.

[0059] FIG. 7 illustrates a third embodiment of a method for monitoring operating conditions in a column capture chromatography system, the column capture chromatography system being configured for cyclical repetitive purification performed on a volume of sample feed comprising at least one product configured to be captured in the column during loading. The volume of the sample feed comes from the same feed (e.g. a large batch or continuous feed). The capture chromatography system includes one single column or, alternatively, several columns coupled in parallel, to which a bioreactor may be connected, and configured to provide a volume of sample feed to the column, the volume of the sample feed being larger than the volumetric capacity of the capture chromatography system. Thus, a repetitive cyclic purification is required. The process includes several steps: a) performing a purification cycle 71, comprising loading a predetermined amount of sample feed onto a column, washing the column, and eluting at least one captured product; b) measuring 72 at least one parameter during elution of at least one captured product indicative of purification capacity within the chromatography system; c) evaluating at least one parameter 74; and if the purification capacity is below an upper predetermined threshold, cleaning the column 75a; d) Repeating steps a) to c). Includes.

[0060] According to some embodiments, the at least one parameter in step b) is selected to be the amount of eluted product in relation to the amount of sample feed loaded on the column. According to some embodiments, the at least one parameter in step b) is selected to be the titer, and the method further comprises determining the peak area during elution and estimating the titer based on the determined peak area.

[0061] According to some embodiments, cyclical repetitive purification is performed on batches of sample feed or on a sample feed provided continuously from a cell culture reactor.

[0062] The present invention also relates to a capture chromatography system, comprising a column configured for cyclical repetitive purification performed on a predetermined amount of a sample feed containing at least one product, and a control unit configured to control the purification in the column, and further configured to perform the method described in relation to Figure 7.

[0063] The methods described above may be implemented in a computer program for controlling a bioprocess purification system. The computer program includes instructions that, when executed on at least one processor, cause the at least one processor to perform the methods described in connection with Figures 5-7. The computer program for controlling a bioprocess purification system may be stored in and carried by a computer readable storage medium.

[0064] Thus, various aspects and embodiments of the present invention can provide a process for running a first chromatography column without manual intervention (e.g., automatically) and for switching to use another chromatography column only when the first chromatography column is exhausted. [Explanation of symbols]

[0065] 11 Cell culture 12 retention 13. Capture 14 Virus inactivation 15 Polish 16 Delivery 17 Automation Layer 20 Sample Feed 21 separation 22 Batching 23 Controlling the upstream process 24 Controlling downstream processes

Claims

1. 1. A method for monitoring operating conditions in a column capture chromatography system configured for cyclical repetitive purification performed on a volume of a sample feed including at least one product configured to be captured in a column during loading, the method comprising: Step a) performing a purification cycle (51) including loading a predetermined amount of sample feed onto the column, washing the column, and eluting at least one of the captured products; step b) measuring (52) at least one parameter during the purification cycle indicative of breakthrough of at least one captured product after the column during loading of a sample feed; step c) reducing the amount of sample feed loaded during loading in a next purification cycle if breakthrough of at least one of the captured products is detected (56a); Step d) Repeat steps a) to c). Including, A method, wherein the loading of the sample in step a) is performed during a loading time (Δt); said loading time is reduced in step c) to prevent breakthrough during the next purification cycle.

2. 2. The method of claim 1, wherein when the loading time has been maintained in step c) for a predetermined number of consecutive purification cycles, the loading time is increased (58a) for the next purification cycle.

3. 3. The method according to claim 1 or 2, wherein the parameter measured in step b) is the signal from a UV detector mounted after the column.

4. 4. The method of claim 1, further comprising monitoring at least one additional parameter measured during elution of at least one captured product indicative of the purification capacity of the column, and increasing the amount of sample feed loaded during loading of the next purification cycle if the purification capacity is decreasing without an indication of breakthrough.

5. 5. The method of claim 4, further comprising, prior to step c), evaluating (54) at least one additional parameter; and cleaning (55a) the column if the purification capacity is below an upper predetermined threshold.

6. The method of claim 5 , wherein the evaluation is a trend analysis of the measured at least one additional parameter over time.

7. 7. The method of claim 5 or 6, wherein the additional parameter is an elution peak area measured upon elution of at least one captured product in relation to the amount of sample feed loaded onto the column.

8. and / or wherein said step of evaluating at least one additional parameter further comprises the step of replacing said column with a new column if said purification capacity falls below a lower predetermined threshold during breakthrough detection (59).

8. The method of claim 5, wherein the cyclical purification is performed on a batch of a sample feed or on a sample feed provided continuously from a cell culture reactor.

9. 1. A method for monitoring operating conditions in a column capture chromatography system configured for cyclical repetitive purification performed on a volume of a sample feed including at least one product configured to be captured in a column during loading, the method comprising: Step a) performing a purification cycle (61) including loading a predetermined amount of sample feed onto the column, washing the column, and eluting at least one of the captured products; step b) measuring (62) at least one parameter during the purification cycle during loading of the sample feed that is indicative of breakthrough of the at least one captured product after the column; and measuring (64) an additional parameter during the purification cycle during elution that is indicative of the amount of the at least one captured product after the column; step c) increasing the amount of sample feed loaded during loading in a next purification cycle if the amount of at least one captured product is decreasing without an indication of breakthrough (68a); Step d) Repeat steps a) to c). A method comprising:

10. 10. The method of claim 9, further comprising, prior to step c), the step of evaluating (65) at least one additional parameter; and cleaning (65a) the column if the purification capacity falls below an upper predetermined threshold.

11. The method of claim 10 , wherein the evaluation is a trend analysis of the measured at least one additional parameter over time.

12. 12. The method of claim 10 or 11, wherein the additional parameter is an elution peak area measured upon elution of at least one captured product in relation to the amount of sample feed loaded onto the column.

13. 13. The method according to any one of claims 9 to 12, wherein the step of evaluating at least one additional parameter further comprises the step (69) of replacing the column with a new column if the purification capacity falls below a lower predetermined threshold during breakthrough detection.

14. 14. The method according to any one of claims 9 to 13, wherein step c) further comprises the step of: reducing (66a) the amount of sample feed loaded during loading in the next purification cycle if breakthrough of at least one captured product is detected.

15. 15. The method of claim 14, wherein the loading of the sample in step a) is performed during a loading time (Δt); the loading time is reduced in step c) to prevent breakthrough during the next purification cycle.

16. 16. The method of claim 15, wherein the loading time is increased (68a) for the next purification cycle when the loading time has been maintained in step c) for at least 25 consecutive purification cycles.

17. The parameter measured in step b) is the signal from a UV detector mounted after the column; and / or 17. The method of any one of claims 9 to 16, wherein the cyclic iterative purification is performed on a batch of a sample feed or on a sample feed provided continuously from a cell culture reactor.

18. 17. A capture chromatography system comprising: a column configured for cyclical iterative purification performed on a volume of a sample feed comprising at least one product; and a control unit configured to control the purification in the column, the control unit being further configured to perform the method of any one of claims 1 to 16.

19. 18. A computer program for monitoring operating conditions in a column chromatography system, the computer program comprising instructions which, when executed on at least one processor, cause the at least one processor to perform the method of any one of claims 1 to 17.

20. 20. A computer readable storage medium embodying a computer program for monitoring operating conditions in a column chromatography system according to claim 19.

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