Adhesive fiber mesh with frame

Abstract

To provide an adhesive fiber mesh with a frame, allowing a fine and uniform fiber mesh that serves as a scaffolding material for cells to be stably incorporated into a device used for cell culture with high quality.SOLUTION: An adhesive fiber mesh with a frame comprises a frame, an arrayed fiber mesh layer arranged in an opening area of the frame, and an adhesive layer in contact with the fiber mesh layer and arranged on an outer periphery of the opening area of the frame. A thickness of the frame is 0.5 to 10 times a total thickness of the fiber mesh layer and the adhesive layer.SELECTED DRAWING: Figure 1-3

Description

[Technical field]

[0001] The present invention relates to an adhesive, aligned fiber mesh structure. [Background technology]

[0002] 2. Description of the Related Art In recent years, fiber meshes having fine and uniform alignment and made of polymeric materials such as polyethylene phthalate and polystyrene have been attracting attention as scaffold materials for cell culture, filtration filters, and the like.

[0003] In particular, in the field of cell culture, 3D cell culture, which mimics the growth morphology of cells in an actual living body in vitro (outside the body), has been attracting attention in recent years, in which cells grow three-dimensionally (3D) to build biological organs. As a scaffold material for realizing this 3D cell culture, layered meshes with fine and uniform alignment have been attracting increasing attention.

[0004] For example, a known scaffold material that enables 3D cell culture is a laminated fiber sheet structure formed by stacking aligned fiber mesh layer structures crossing each other at 90° lengthwise and widthwise and attaching the stacked fiber sheet to a frame, as shown in Figure 18B of Patent Document 1. Cells are cultured on this laminated fiber sheet with alignment.

[0005] Fig. 6 is a schematic diagram showing the structure of an example of a framed fiber mesh proposed in Patent Document 1, in which a fiber mesh layer having a conventional alignment is attached to a frame. Figs. 7-1 to 7-4 are schematic diagrams showing the steps of an example of a manufacturing method for incorporating a fiber mesh part of a conventional framed fiber mesh into a device used for cell culture or the like.

[0006] First, the structure of a conventional framed fiber mesh will be described with reference to FIG. In FIG. 6, the framed fiber mesh 30 is a 3D structure formed by stacking alternately in the Z direction a layer structure in which fibers 22 made of a polymeric material such as polystyrene, polyethylene, or polyethylene terephthalate are arranged parallel to the X direction and at a uniform pitch, and a layer structure in which fibers 21 made of the same polymeric material are arranged parallel to the Y direction and at a uniform pitch, and then affixed to a frame 50.

[0007] Next, an example of a manufacturing method for incorporating a fiber mesh portion serving as a scaffold for cells from a conventional framed fiber mesh 30 into a device used for cell culture will be described below with reference to Figs. 7-1 to 7-4. (1) First, as shown in Fig. 7-1, adhesive 8 is applied to a device 5 used for cell culture in a shape that matches the outer periphery of a fiber mesh portion of a framed fiber mesh 30. Next, the framed fiber mesh 30 is aligned so that the fibers 21 (22) attached to the frame 50 face the device 5, and the fibers 21 (22) are placed in contact with the adhesive 8. Instead of applying adhesive 8, an adhesive tape may be attached. (2) Next, as shown in FIG. 7-2, the fibers 21 (22) inside the frame 50 are pressed by the pressing member 6 to bond the fibers 21 (22) and the adhesive 8 together. (3) Next, as shown in FIG. 7-3, the pressing member 6 is lifted up. (4) Next, as shown in FIG. 7-4, the frame 50 is lifted up. As a result, the frame 50 and the fibers 22 (21) are separated, and the fiber mesh portion of the frame-attached fiber mesh 30 is incorporated into the device 5, completing a device for cell culture. [Prior art documents] [Patent documents]

[0008] [Patent Document 1] JP 2020-79460 A Summary of the Invention [Problem to be solved by the invention]

[0009] However, in the case of the framed fiber mesh of Patent Document 1, the manufacturing method shown in Figures 7-1 to 7-4 in which a fiber mesh part serving as a scaffolding material for cells is incorporated into a device used for cell culture may cause the following problem: In the process shown in Figure 7-2 in which the pressing member 6 presses the fibers 21 (22) inside the frame 50 to bond the fibers 21 (22) to the adhesive 8, the adhesive 8 climbs over the fibers 21 (22) and adheres to the frame 50 and the pressing member 6 as shown in Figure 8-1.

[0010] As a result, when the pressing member 6 is pulled up in the process shown in Fig. 7-3, the adhesive 8 attached to the pressing member 6 and the fibers 21 (22) with which the adhesive 8 is entangled are also pulled up together with the pressing member 6. For this reason, after the pressing member 6 is pulled up, the fiber mesh 21 (22) is torn or its alignment is disturbed, as shown in the circle G in Fig. 8-2.

[0011] 7-4, when the frame body 50 is pulled up, the adhesive 8 attached to the frame body 50 and the fibers 21 (22) with which the adhesive 8 is entangled are also pulled up. Therefore, after the frame body 50 is pulled up, the fiber mesh 21 (22) is torn or its alignment is disturbed, as shown in the circle H in FIG. 8-3.

[0012] For this reason, it has been difficult to stably and with good quality incorporate a fiber mesh having fine and uniform alignment to serve as a scaffolding material for cells into a device used for cell culture.

[0013] Furthermore, this problem is not limited to when a fiber mesh having a fine and uniform alignment is incorporated into a device used for cell culture, but also occurs when a fiber mesh having a fine and uniform alignment is incorporated into other devices such as filtration filters.

[0014] Based on the above, an object of the present invention is to provide an adhesive fiber mesh with a frame that can be used as a scaffolding material in 3D cell culture, a high-performance filtration filter, etc., and that has fine and uniform alignment and can be stably and with good quality incorporated into a device. [Means for solving the problem]

[0015] In order to solve the above problems, the present invention provides a frame-attached adhesive fiber mesh comprising a frame and The frame body has a fiber mesh layer having an alignment and arranged in the opening region of the frame body, and an adhesive layer in contact with the fiber mesh layer and arranged on the outer periphery of the opening region of the frame body, and the thickness of the frame body is 0.5 to 10 times the total thickness of the fiber mesh layer and the adhesive layer combined.

[0016] A method for manufacturing a cell culture device according to the present invention includes the steps of aligning and placing the above-mentioned frame-attached adhesive fiber mesh on a substrate so that the adhesive layer and the substrate face each other, pressing the fiber mesh layer inside the opening area of ​​the frame with a pressing member to adhere the adhesive layer to the substrate, and lifting the pressing member and the frame vertically above the substrate to transfer the adhesive layer and the fiber mesh layer onto the substrate. Effect of the Invention

[0017] According to the frame-attached adhesive fiber mesh of the present invention, in the manufacturing process of incorporating the fiber mesh portion, which serves as a scaffolding material for cells, into a device used for cell culture or the like, no adhesive layer adheres to the pressing member or frame. This makes it possible to stably and with good quality incorporate a fiber mesh having fine and uniform alignment into a device used for cell culture. This makes it possible to stably and with good quality manufacture and provision of devices for 3D cell culture, high-performance filtration filters, and the like. [Brief description of the drawings]

[0018] [Figure 1-1] 1 is a schematic diagram showing the structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 1-2]1 is a schematic diagram showing the structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 1-3] 1 is a schematic diagram showing a cross-sectional structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 2-1] FIG. 2 is a schematic diagram showing a step of an example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device. [Figure 2-2] FIG. 2 is a schematic diagram showing a step of an example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device. [Figure 2-3] FIG. 2 is a schematic diagram showing a step of an example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device. [Figure 2-4] FIG. 2 is a schematic diagram showing a step of an example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device. [Figure 2-5] FIG. 2 is a schematic diagram showing a step of an example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device. [Figure 2-6] FIG. 2 is a schematic diagram showing an example of the cross-sectional structure of the frame-attached adhesive fiber mesh according to embodiment 1 after a fiber mesh portion thereof has been incorporated into a device. [Figure 2-7] FIG. 2 is a schematic diagram showing an example of the structure of a device after a fiber mesh portion of a frame-attached adhesive fiber mesh according to embodiment 1 has been incorporated therein. [Figure 3-1] 1 is a schematic diagram showing the structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 3-2] 1 is a schematic diagram showing a cross-sectional structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 4-1] 1 is a schematic diagram showing the structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 4-2] 1 is a schematic diagram showing a cross-sectional structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 5-1]1 is a schematic diagram showing the structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 5-2] 1 is a schematic diagram showing a cross-sectional structure of an example of a frame-attached adhesive fiber mesh according to embodiment 1. FIG. [Figure 6] FIG. 1 is a schematic diagram showing an example of a conventional framed fiber mesh. [Figure 7-1] FIG. 1 is a schematic diagram showing one step of an example of a manufacturing method for incorporating a fiber mesh portion of a conventional frame-attached adhesive fiber mesh into a device. [Figure 7-2] FIG. 1 is a schematic diagram showing one step of an example of a manufacturing method for incorporating a fiber mesh portion of a conventional frame-attached adhesive fiber mesh into a device. [Figure 7-3] FIG. 1 is a schematic diagram showing one step of an example of a manufacturing method for incorporating a fiber mesh portion of a conventional frame-attached adhesive fiber mesh into a device. [Figure 7-4] FIG. 1 is a schematic diagram showing one step of an example of a manufacturing method for incorporating a fiber mesh portion of a conventional frame-attached adhesive fiber mesh into a device. [Figure 8-1] FIG. 1 is a schematic diagram showing an example of a problem that occurs when a fiber mesh portion of a conventional frame-attached adhesive fiber mesh is incorporated into a device. [Figure 8-2] FIG. 1 is a schematic diagram showing an example of a problem that occurs when a fiber mesh portion of a conventional frame-attached adhesive fiber mesh is incorporated into a device. [Figure 8-3] FIG. 1 is a schematic diagram showing an example of a problem that occurs when a fiber mesh portion of a conventional frame-attached adhesive fiber mesh is incorporated into a device. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0019] The frame-attached adhesive fiber mesh of the first aspect comprises a frame, a fiber mesh layer having an alignment and arranged in an opening region of the frame, and an adhesive layer in contact with the fiber mesh layer and arranged on the outer periphery of the opening region of the frame, and the thickness of the frame is 0.5 to 10 times the total thickness of the fiber mesh layer and the adhesive layer combined.

[0020] In the second aspect of the frame-attached adhesive fiber mesh of the first aspect, the fiber mesh layer may be composed of a first fiber layer in which first fibers made of a polymeric material are arranged in parallel, and a second fiber layer in which a second fiber layer made of a polymeric material is arranged in parallel on the first fiber layer so as to intersect with the first fiber layer.

[0021] The frame-attached adhesive fiber mesh according to a third aspect is the above-mentioned first aspect or the above-mentioned second aspect, in which the adhesive layer may be made of a silicone-based adhesive.

[0022] The frame-attached adhesive fiber mesh according to a fourth aspect is the same as that of the third aspect, and the adhesive layer may be configured such that fibers made of a silicone-based adhesive are arranged parallel to each other.

[0023] The frame-attached adhesive fiber mesh of the fifth aspect is any one of the first to fourth aspects, in which an adhesive layer may also be disposed between the fiber mesh layer and the frame.

[0024] The frame-attached adhesive fiber mesh of the sixth aspect is any one of the first to fifth aspects above, in which a second frame may be arranged on the opposite side of the frame via the fiber mesh layer.

[0025] A method for manufacturing a cell culture device according to the seventh aspect includes the steps of aligning and placing a frame-attached adhesive fiber mesh according to any one of the first to sixth aspects on a substrate so that the adhesive layer faces the substrate, pressing the fiber mesh layer inside the opening area of ​​the frame with a pressing member to adhere the adhesive layer to the substrate, and lifting the pressing member and the frame vertically above the substrate to transfer the adhesive layer and the fiber mesh layer onto the substrate.

[0026] Hereinafter, a frame-attached adhesive fiber mesh according to an embodiment will be described with reference to the drawings, in which substantially the same components are given the same reference numerals.

[0027] (Embodiment 1) <Structure of adhesive fiber mesh with frame> 1-1 to 1-3 are schematic diagrams showing an example of the structure of a frame-attached adhesive fiber mesh according to embodiment 1. An example of the structure of a frame-attached adhesive fiber mesh according to embodiment 1 will be described with reference to Figs. 1-1 to 1-3.

[0028] Figure 1-1 is a schematic plan view of a frame-attached adhesive fiber mesh 1 viewed from above (Z direction), Figure 1-2 is a schematic oblique view viewed from above at an angle, and Figure 1-3 is a schematic cross-sectional view showing the cross-sectional structure viewed in the AA' direction of Figure 1-1.

[0029] As shown in Figures 1-1 and 1-2, the frame-attached adhesive fiber mesh 1 is composed of a frame 2, an aligned fiber mesh layer 3 formed on the frame 2, and an adhesive layer 4 in contact with the aligned fiber mesh 3 and formed on the outer periphery of the opening area of ​​the frame 2.

[0030] In embodiment 1, the aligned fiber mesh layer 3 is composed of a layer structure in which the first fibers 11 are formed at equal intervals parallel to the X direction, and a layer structure in which the second fibers 12 are formed at equal intervals parallel to the Y direction on top of the layer structure formed by the first fibers 11. The oriented fiber mesh layer 3 was produced by a process of forming first fibers 11 made of polystyrene and having a diameter of 20 μm in parallel to the X direction at intervals of 50 μm, and then forming second fibers also made of polystyrene and having a diameter of 20 μm in parallel to the Y direction at intervals of 50 μm so that the cross angle with the first fibers is 90°.

[0031] 1-1 and 1-2 show an example in which the crossing angle between the first fibers 11 and the second fibers 12 is 90°. The crossing angle between the first fibers and the second fibers 12 is not limited to this value, and is preferably in the range of 30° to 150°. Further, although the material of the first fibers 11 and the second fibers 12 is polystyrene in the above example, the material is not limited to polystyrene and may be other polymeric materials such as polyethylene, polyethylene phthalate, or the like.

[0032] <Frame> As shown in Fig. 1-3, in the frame-attached adhesive fiber mesh 1, the frame 2 and the adhesive layer 4 are formed on (in the Z direction) an aligned fiber mesh layer 3 composed of first fibers 11 and second fibers 12. The thickness T of the frame 2 is a structure in which, where S is the total thickness of the aligned fiber mesh layer 3 and the adhesive layer 4, it falls within a range of T = 0.5 x S to T = 10 x S.

[0033] The reasons and effects thereof will be explained later in connection with an example of a manufacturing method for incorporating a fiber mesh portion of a frame-attached adhesive fiber mesh into a device.

[0034] In the structure of the frame-attached adhesive fiber mesh 1 relating to embodiment 1, the frame 2 was made of polyethylene terephthalate and had a thickness of 1 mm, and the adhesive layer 4 was made of a silicone-based adhesive having a thickness of 0.075 mm.

[0035] In this configuration, the thickness T of the frame 2 is T = 1 mm. The total thickness S of the aligned fiber mesh layer 3 and the adhesive layer 4 is S = [(thickness of the first fibers 11 0.05 mm) + (thickness of the second fibers 12 0.05 mm) + (thickness of the adhesive layer 4 0.075 mm)] = 0.175. Therefore, the relationship between T and S is T = 5.7 × S, which satisfies the range indicating the relationship between T and S described above.

[0036] The material of the frame 2 is not limited to polyethylene terephthalate, but may be other resin materials or metal materials such as aluminum and SUS. When a fiber mesh is used as a scaffold for cell culture, the material of the adhesive layer 4 is preferably a silicone adhesive in consideration of biocompatibility, but is not limited thereto.

[0037] <How to embed into device> Figures 2-1 to 2-5 are schematic cross-sectional views showing the steps of an example of a manufacturing method for incorporating a fiber mesh part of the framed adhesive fiber mesh according to embodiment 1 into a device. Figures 2-6 to 2-7 are schematic cross-sectional views showing an example of a cross-sectional structure after incorporating a fiber mesh part of the framed adhesive fiber mesh according to embodiment 1 into a device. An example of a manufacturing method for incorporating a fiber mesh portion of the frame-attached adhesive fiber mesh according to embodiment 1 into a device will be described with reference to Figs. 2-1 to 2-7.

[0038] (1) First, the frame-attached adhesive fiber mesh 1 is aligned and placed in a predetermined position on the device 5 shown in Fig. 2-1 where the fiber mesh is to be attached, with the adhesive layer 4 facing the device 5. Although not shown in Figs. 2-1 to 2-7, in the case of a device for cell culture, a flow path for circulating a cell culture medium is often formed in the location where the fiber mesh of the device 5 is to be attached.

[0039] (2) Next, as shown in Fig. 2-2, the pressing member 6 is aligned on the aligned fiber mesh layer 3 so as not to overlap with the formation position of the frame body 2, and then pressed. The size of the pressing member 6 is designed to be large enough to fit inside the frame body 2, i.e., inside the opening region of the frame body 2, and large enough to press the adhesive layer 4.

[0040] (3) By pressing the alignable fiber mesh layer 3 with the pressing member 6, the adhesive layer 4 comes into contact with the device 5, as shown in part E enclosed in a circle in FIG. 2-3. Thereafter, the pressing member 6 is further pressed into the frame 2 until the contact surface between the pressing member 6 and the aligned fiber mesh layer 3 is lower than the upper surface of the frame 2. As a result, since the height of the frame 2 remains unchanged, a shear stress is generated between the frame 2 and the end of the aligned fiber mesh layer 3 pressed by the pressing member 6.

[0041] (4) At that time, if the thickness of the frame body 2 is within the range of 0.5 to 10 times the total thickness of the aligned fiber mesh layer 3 and the adhesive layer 4 combined, the generated shear stress will cause the aligned fiber mesh layer 3 to break between the end of the aligned fiber mesh layer 3 pressed by the pressing member 6 and the frame body 2, as shown in area F surrounded by a circle in Figure 2-4. If the thickness of the frame body 2 is less than 0.5 times the total thickness of the front fiber mesh layer 3 and the adhesive layer 4 combined, the shear stress generated between the end of the aligned fiber mesh layer 3 pressed by the pressing member 6 and the frame body 2 will be small, and the aligned fiber mesh layer 3 may not break. Conversely, if the thickness of the frame 2 exceeds 10 times the total thickness of the front fiber mesh layer 3 and the adhesive layer 4, the contact position of the adhesive layer 4 on the device 5 will vary greatly, making it difficult to incorporate the fiber mesh at a predetermined location on the device 5. Furthermore, the variation in the contact position of the adhesive layer 4 on the device 5 will disrupt the fine and uniform alignment of the alignable fiber mesh layer 3. As shown in FIG. 2-4, after the oriented fiber mesh layer 3 is broken, the pressing member 6 is pulled upward.

[0042] (5) Next, as shown in FIG. 2-5, the frame 2 is pulled upward.

[0043] Through the above steps, a structure is completed in which the alignable fiber mesh layer 3 is incorporated through the adhesive layer 4 while maintaining fine and uniform alignment at a predetermined position of the device 5, as shown in the schematic cross-sectional diagram of the structure shown in Figure 2-6 and the schematic plan view of the top surface of the device 5 shown in Figure 2-7.

[0044] [effect] When this frame-attached adhesive fiber mesh 1 is used, when the fiber mesh portion is incorporated into a device, a process is carried out in which the aligned fiber mesh layer 3, which has a fine and uniform alignment, is broken by shear stress, so the pressure with which the pressing member 6 presses the aligned fiber mesh layer 3 is very small. Therefore, there is no risk of the adhesive layer 4 being spread out by the pressure of the pressing member 6 when it is pressed, and adhering to the frame 2 or the pressing member 6, as in the conventional example. As a result, the alignable fiber mesh layer 3 is free from tearing or disturbance in alignment, and a fiber mesh having fine and uniform alignment can be stably incorporated into a device with good quality.

[0045] (Embodiment 2) <Adhesive fiber mesh with frame> Next, a frame-attached adhesive fiber mesh according to embodiment 2 will be described with reference to Figures 3-1 and 3-2. Figures 3-1 and 3-2 are schematic diagrams showing an example of the structure of a frame-attached adhesive fiber mesh 1a according to embodiment 2.

[0046] FIG. 3-1 is a schematic plan view of a frame-attached adhesive fiber mesh 1a viewed from above (Z direction), and FIG. 3-2 is a schematic cross-sectional view showing the cross-sectional structure viewed in the BB' direction of FIG. 3-1.

[0047] As shown in Figure 3-1, the frame-attached adhesive fiber mesh 1a of embodiment 2 is composed of a frame 2, an aligned fiber mesh layer 3 formed on the frame 2, and an adhesive layer 4 that is in contact with the aligned fiber mesh layer 3 and is formed between the frame 2 and the aligned fiber mesh layer 3 in the thickness direction (Z direction) as shown on the outer periphery of the opening region of the frame 2 and in part D surrounded by a circular frame in Figure 3-2. Furthermore, since the area of ​​the adhesive layer 4 formed between the frame body 2 and the aligned fiber mesh layer 3 is very small, it has almost no effect on the shear stress generated between the end of the aligned fiber mesh layer 3 pressed by the pressing member 6 shown in Figure 2-3 and the frame body 2. Therefore, in the frame-attached adhesive fiber mesh 1a of embodiment 2, it is preferable that the thickness of the frame 2 be within the range of 0.5 to 10 times the total thickness of the aligned fiber mesh layer 3 and the adhesive layer 4 combined.

[0048] According to this configuration, the adhesive layer 4 is formed between the frame body 2 and the aligned fiber mesh layer 3 in the thickness direction (Z direction), thereby making the bond between the frame body 2 and the aligned fiber mesh layer 3 on the frame body 2 stronger. As a result, in the process shown in Figure 2-3 in which the pressing member 6 is pressed to generate shear stress in the aligned fiber mesh layer 3, it is possible to suppress even slight distortion caused by the shear stress in the aligned fiber mesh layer 3 on the frame 2. This makes it possible to stably incorporate a fiber mesh having very uniform alignment into a device with good quality.

[0049] (Embodiment 3) <Adhesive fiber mesh with frame> Next, a frame-attached adhesive fiber mesh according to embodiment 3 will be described with reference to Fig. 4-1 and Fig. 4-2. Fig. 4-1 and Fig. 4-2 are schematic diagrams showing an example of the structure of a frame-attached adhesive fiber mesh 1b according to embodiment 3.

[0050] FIG. 4-1 is a schematic perspective view of a frame-attached adhesive fiber mesh 1b as viewed obliquely from above (Z direction), and FIG. 4-2 is a schematic cross-sectional view as viewed in the CC' direction of FIG. 4-1.

[0051] As shown in Figures 4-1 and 4-2, the frame-attached adhesive fiber mesh 1b of embodiment 3 is composed of a frame 2, an alignable fiber mesh layer 3 formed on the frame 2, and adhesive fibers 13 and 14 formed parallel to the X direction and parallel to the Y direction in contact with the alignable fiber mesh layer 3 on the outer periphery of the opening region of the frame 2. In addition, in Figures 4-1 and 4-2, adhesive fiber 13 and adhesive fiber 14 are shown to have a configuration in which two fibers are formed in parallel, but this configuration is not limited to that, and the adhesive fiber may also have a structure composed of one fiber or three or more fibers.

[0052] According to this structure, the fibrous adhesive fibers 13 and the adhesive fibers 14 can be formed using the same equipment as that for forming the aligned fiber mesh layer 3. This reduces the manufacturing cost of the frame-attached adhesive fiber mesh 1b, making it possible to provide it at low cost.

[0053] (Embodiment 4) <Adhesive fiber mesh with frame> Next, a frame-attached adhesive fiber mesh according to embodiment 4 will be described with reference to Fig. 5-1 and Fig. 5-2. Fig. 5-1 and Fig. 5-2 are schematic diagrams showing an example of the structure of a frame-attached adhesive fiber mesh 1c according to embodiment 4.

[0054] FIG. 5-1 is a schematic perspective view of a frame-attached adhesive fiber mesh 1c as viewed obliquely from above (Z direction), and FIG. 5-2 is a schematic cross-sectional view as viewed from the II' direction in FIG. 5-1.

[0055] As shown in Figures 5-1 and 5-2, the frame-attached adhesive fiber mesh 1c of embodiment 4 is composed of a frame 2, an aligned fiber mesh layer 3 formed on the frame 2, an adhesive layer 4 in contact with the aligned fiber mesh layer 3 and formed on the outer periphery of the opening area of ​​the frame 2, and a frame 7 formed on the opposite side of the frame 2 via the aligned fiber mesh layer 3.

[0056] In the structure of the frame-attached adhesive fiber mesh 1c relating to embodiment 4, the frame 2 is made of polyethylene phthalate and has a thickness of 1 mm, and the frame 7 is made of the same polyethylene phthalate resin as the frame 2 and has a thickness of 5 mm.

[0057] According to this configuration, by forming the frame 7 as a second frame on the opposite surface of the frame 2 with the aligned fiber mesh layer 3 interposed therebetween, the rigidity of the frame-attached adhesive fiber mesh 1c is increased. As a result, the handleability of the framed adhesive fiber mesh 1c is improved, which makes it possible to prevent quality defects such as tearing of the aligned fiber mesh layer 3 during handling in the manufacturing process. This reduces the manufacturing cost of the framed adhesive fiber mesh 1c, making it possible to provide the framed adhesive fiber mesh 1c at low cost.

[0058] In addition, the present disclosure includes appropriate combinations of any of the various embodiments and / or examples described above, and can achieve the effects of each embodiment and / or example. [Industrial Applicability]

[0059] The frame-attached adhesive fiber mesh of the present invention makes it possible to stably incorporate a fiber mesh having fine and uniform alignment into a device with good quality without tearing or disturbance of the fiber mesh layer, which allows devices used as 3D cell culture devices, high-performance filtration filters, etc. to be manufactured with good quality and at low cost. [Explanation of symbols]

[0060] 1. Adhesive fiber mesh with frame 2 frame 3. Aligned fiber mesh layer 4 Adhesive layer 5 Devices 6 Pressing member 7 Second Frame 8 Adhesive 11 First Fiber 12 Second Fiber 21 Textiles 22 Textiles 30 Fiber mesh with frame 50 frame S: Total thickness of the first fiber, the second fiber, and the adhesive, etc. T Frame thickness

Claims

1. A frame body, a fiber mesh layer having an alignment and disposed in the opening region of the frame; an adhesive layer in contact with the fiber mesh layer and disposed on an outer periphery of the opening region of the frame; Equipped with The thickness of the frame is 0.5 to 10 times the total thickness of the fiber mesh layer and the adhesive layer. Adhesive fibre mesh with frame.

2. The fiber mesh layer is a first fiber layer including first fibers made of a polymeric material arranged in parallel; a second fiber layer, the second fibers being made of the polymer material and arranged in parallel on the first fiber layer so as to cross the first fiber layer; It is made up of:

2. The frame-attached adhesive fiber mesh according to claim 1.

3. The adhesive layer is made of a silicone adhesive.

3. The frame-attached adhesive fiber mesh according to claim 1 or 2.

4. The adhesive layer is configured such that fibers made of a silicone-based adhesive are arranged parallel to each other. The frame-attached adhesive fiber mesh according to claim 3.

5. The adhesive layer is also disposed between the fiber mesh layer and the frame body. The frame-attached adhesive fiber mesh according to any one of claims 1 to 4.

6. A second frame body is disposed on the opposite side of the frame body via the fiber mesh layer. The frame-attached adhesive fiber mesh according to any one of claims 1 to 5.

7. A step of aligning and disposing the frame-attached adhesive fiber mesh according to any one of claims 1 to 6 on a substrate so that the adhesive layer faces the substrate; a step of pressing the fiber mesh layer inside the opening region of the frame with a pressing member to bond the adhesive layer to the substrate; a step of lifting the pressing member and the frame in a vertical direction of the substrate to transfer the adhesive layer and the fiber mesh layer onto the substrate; A method for manufacturing a cell culture device comprising the steps of:

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