A method for decomposing and purifying residual pesticides in crops, soil, processed foods, beverages, raw materials for herbal medicines, and sewage residues, and for decomposing and purifying soil nitrate nitrogen, using ascomycete mycorrhizal fungi and wood-rotting fungi.

Abstract

To provide a technology that balances increased food production and control of environmental pollution that can cope with the increasing population.SOLUTION: After the agrochemicals used on the crops fulfilled their function, the ascomycetes white wood-rotting fungus having the characteristics of mycorrhizal fungus is used to decompose the agrochemicals remaining on the crops and soil.SELECTED DRAWING: Figure 4

Description

[Technical field]

[0001] This invention relates to a technology for decomposing and detoxifying residual components of chemical fertilizers and pesticides used in agricultural plants and crop cultivation worldwide using the white truffle Tuber fungus, a wood-rotting mycorrhizal fungus, to produce safe and reliable food, processed foods, beverages, tea, and herbal medicines. [Background technology]

[0002] The 20th century was a century in which the world built a carbon society, wasted the earth's limited resources, and sought comfortable lives and prosperity. However, as we entered the 21st century, many problems and issues have appeared, as if the burden of all this has suddenly surfaced. To make matters worse, the new coronavirus of 2020 has shaken the foundations of human society, and we are now faced with the challenge of being unable to foresee the future of humanity unless we solve the biggest problem of overcoming epidemics. All industries cannot exist without human health. In the future, society will become one in which health is maintained by one's own "immunity," and health awareness will likely increase very rapidly in the future.

[0003] At this time, the problem of "pesticide residues" in the food we eat every day becomes an issue. Completely pesticide-free food would be ideal, but it would not be enough to feed the current population. Malnutrition immediately leads to a weakened "immunity," and in poor countries, epidemics and starvation lead to tragic situations. In order to prevent this starvation and weakened immunity, we must secure food by using a lot of pesticides and chemical fertilizers.

[0004] Vegetables and fruits grown in Japan are sprayed with pesticides at least six times before harvest, and in some cases more than 60 times, with the amount of pesticide used per hectare (2010) being 12.1 kg, the third highest in the world after China and South Korea. If you eat crops grown under such conditions, you will inevitably ingest residual pesticides, but these residual pesticides play a major role in the human immune system, and a decline in "immunity" cannot be avoided no matter what you do. The new coronavirus has demonstrated that the strength of the immune system determines the survival of humanity.

[0005] It is an urgent task to develop technology that can simultaneously satisfy two of the most difficult problems for human survival: producing safe, pesticide-free food that does not weaken the immune system, while securing enough food to meet the future population growth. In today's world agriculture, large amounts of pesticides and chemical fertilizers are used to feed the growing world population, and the problem of residual pesticides (herbicides) and residual nitrate nitrogen is not limited to food and soil. Antibiotics in livestock feed and contamination by spraying herbicides on non-agricultural land are serious problems that affect rivers, oceans, the global environment, and biological ecosystems. In particular, the new coronavirus that shocked the entire world in 2020 has exposed the problem of weakening human immunity, and the world must solve a new challenge: fighting epidemics.

[0006] The world population, which was approximately 1.6 billion in 1900 at the beginning of the 20th century, has rapidly increased to 7.7 billion in 2019. This rapid population growth was made possible by the increased food production through chemical fertilizers and pesticides invented by Haber-Bosch in the early 1900s using ammonia synthesis. It is said that in order to support the population that is expected to grow in the future, it will be necessary to use more fertilizers and pesticides in the future. Poor growth and weakening due to high temperature damage caused by global warming induces a large outbreak of pests. This environmental change has progressed to the point where it threatens the appropriate production of crops in suitable locations, and in this situation, more chemical fertilizers, pesticides, and herbicides have to be used to ensure yields. This has further increased the energy consumption for fertilizer production using the Haber-Bosch method, and it is no exaggeration to say that many of the important problems and challenges that humanity must solve in the 21st century, such as oil depletion, promotion of global warming due to residual nitrate nitrogen in the soil, destruction of biological ecosystems, and disappearance of tropical rainforests, are agricultural problems.

[0007] It is said that current food production would drop by 60% if heavy fertilizer cultivation and pesticide use were not implemented, and countries around the world are prepared to continue using pesticides from the standpoint of stable food production. In particular, grain cultivation is a "battle against weeds," and the most commonly used pesticide is herbicide. The amount of herbicide used is expected to increase in the future, going in the opposite direction to food safety and security, but if we do not do so, we will not be able to feed the growing population. The world of the 21st century is being forced to simultaneously solve food safety and hunger, pesticides and immunity. Even if a cure or vaccine for the new coronavirus is created, residual pesticides will remain dormant in the body and cause immunity to be lost, and the same thing could happen again with the next epidemic.

[0008] Various methods have been proposed as means for treating residual pesticides and accumulated chemical fertilizers in the soil of farmland, etc., but physical methods (e.g., Patent Documents 1-3) require the treatment of large amounts of soil and the use of complex equipment, and are not widely used in practice. In addition, chemical treatment methods (e.g., Patent Document 4) require the spraying of the required amount over a wide area, and the treatment agent remains in the soil as it is, so they cannot be used for some crops. Furthermore, treatment methods using microorganisms have been proposed, not only for specific pesticides (Patent Documents 5-7) but also for general-purpose microorganisms (Patent Document 8), but because the growth of these microorganisms is not taken into consideration, they eventually die out and the effect is limited.

[0009] On the other hand, residual pesticides in harvested crops must be removed after ensuring the safety of the crops, and washing with water is the main method used, but it is difficult to remove them completely, and this method is particularly ineffective at removing pesticides that remain inside the crops while they are growing. Thus, there has been a demand for a simple and safe method of removing harmful substances from nearby environments such as agricultural crops and their farmland. [Prior art documents] [Patent documents]

[0010] [Patent Document 1] JP 2019-181403 A [Patent Document 2] JP 2016-010768 A [Patent Document 3] JP 2013-220419 A [Patent Document 4] JP 2010-017219 A [Patent Document 5] JP 2011-031180 A [Patent Document 6] JP 2010-017086 A [Patent Document 7] JP 2005-065614 A [Patent Document 8] JP 2000-232875 A Summary of the Invention [Problem to be solved by the invention]

[0011] As mentioned above, humans have developed pesticides to secure food to meet the growing population, and by using ingredients that contain pesticide residues, almost all food, processed foods, and beverages have become contaminated with pesticides. When food is produced to secure food, these pesticides weaken the human immune system, have a major impact on the biological ecosystem, and are even threatening the global environment.

[0012] In nature, there is a law of nature that toxic substances produced by living organisms are always decomposed, detoxified, and purified by the living organism. Humans are the only ones who create chemical compounds that cannot be decomposed or detoxified. In principle, when science creates toxic pesticides, it should also develop "antidotes" that decompose, detoxify, and purify them in the same way as nature does, but the root cause of the current residue problem is the result of pursuing profits without doing this. When the inventor considered the above law of nature, he came to the conclusion that if we cannot continue to secure food without using pesticides, the problem cannot be solved unless we "decompose and detoxify pesticides" in accordance with the law of nature. [Means for solving the problem]

[0013] The inventor has previously considered "completely pesticide-free cultivation" and "reduced fertilizer and pesticide-free cultivation" and has filed three patent applications (Patent Application No. 2019-164530, Patent Application No. 2019-164410, Patent Application No. 2020-142873). However, the current situation is that, conversely, it is predicted that the production and use of pesticides will continue to increase in the future, and when looking at agriculture around the world, it is predicted that "reduced fertilizer, reduced pesticide and pesticide-free cultivation", which requires advanced technology, will result in reduced yields and food shortages in some countries. Therefore, the inventors have come up with the idea of ​​using pesticides, but then carrying out an antidote treatment to break down, detoxify and purify residual pesticides from food, processed foods, beverages and the environment, thereby preventing damage to the human immune system and environmental damage while still using pesticides.

[0014] In order to completely decompose, detoxify, and purify residual pesticides from food, processed foods, beverages, and the environment, it is necessary to remove not only the pesticides attached to the surface of crops, but also the pesticides accumulated inside growing plants and crops, but no such technology has been known so far. Moreover, since the problem of residual pesticides is now a global issue, the technology must be applicable worldwide.

[0015] In nature, almost all of the toxic compounds produced as bioactive substances by organisms in nature as metabolites are decomposed and detoxified by wood-rotting fungi and white wood-rotting fungi as the primary decomposers, and the soil of the Earth's nature is maintained by this purification system. The inventor, focusing on the laws and providence of the Earth's nature, thought that if this purification system of nature is introduced to the decomposition and detoxification of residual pesticides, it would be possible to produce food that is safe and secure, while also being able to produce food that can meet the current population and the expected population increase in the future. Furthermore, the problem of pesticide residues in the food cycle does not only affect crops, but also meat, eggs, and dairy products that are fed feed containing pesticide residues, and seafood and fish that are eaten from seawater contaminated with pesticides.The pollution has progressed to the point where there is no safe and secure food on Earth.

[0016] The residual pesticides that affect the ecosystem of the area around the field are not human-oriented residual concentration standards at the time of harvesting and shipping, but the problem of pesticides remaining in the area during cultivation. From the above perspective, the inventor thought that it would be impossible to conserve the biological ecosystem unless the residual pesticides at harvesting, as well as the residual pesticides during the growth process of crop cultivation, are decomposed, detoxified, and purified. In other words, if the residual pesticides are decomposed and detoxified quickly after the effect of pesticide spraying or herbicide spraying is observed, all of the above problems should be solved. Furthermore, by decomposing and removing the residual pesticides in agricultural crops that are the raw materials for processed food ingredients, safe and secure processed foods, beverages, teas, etc. can be produced.

[0017] As mentioned earlier, the problem of residual pesticides in the food cycle does not only affect crops, but also meat, eggs, and dairy products that are eaten with feed containing residual pesticides, and seafood and fish that are eaten with seawater contaminated with pesticides.The pollution has progressed to the point where there is no safe food on Earth, but the above pollution can be controlled by decomposing and removing the residual pesticides in the crops that are used to make feed, and in fields and other areas where pesticides have been sprayed.

[0018] In order to decompose and detoxify residual pesticides, the objective of the present invention cannot be achieved unless the fungus has better fungicide resistance than the Pezizales sp. fungus used in the prior application of the reduced fertilizer, reduced pesticide and pesticide-free cultivation method (Patent Application No. 2019-164530). Therefore, the inventor assumed that ascomycete wood-rotting mycorrhizal fungi that form fruit bodies in the soil would have resistance to various environmental conditions and bacteria in the soil. This is because, in order to form the next generation of fruit bodies in the soil, it is necessary to decompose and purify the various harmful components, which contain strong antibacterial properties as well as biologically active substances such as antibiotics and biological toxins produced by various microorganisms living in the soil.

[0019] From this perspective, the present inventor has pinned his hopes on the "white truffle" Tuber magnatum, which belongs to the Tuber ssp., an "ascomycete white wood-rotting mycorrhizal fungus" used in the "reduced fertilizer, reduced pesticide and pesticide-free cultivation". This white truffle Tuber has been used as an edible fungus in luxury cuisine as the best mushroom for centuries in Western countries, is harmless to humans and animals, and grows wild all over the world. The present inventor has focused on this white truffle Tuber and conducted extensive tests to elucidate its characteristics, and has elucidated 31 unique characteristics not seen in other microorganisms, as shown in Table 1 below.

[0020] [Table 1]

[0021] The reasons for focusing on the white truffle Tuber fungus are that it is a white wood-rotting fungus that can break down lignin and is the microorganism with the most powerful decomposition power on Earth; there are two types of white wood-rotting fungi: ascomycetes and basidiomycetes; however, ascomycetes do not have fungi that kill plants like basidiomycetes, and they are harmless to plants as they break down residual pesticides in growing crops; although it is an "ascomycete white wood-rotting fungus," it has evolved into a mycorrhizal fungus that helps plant growth and forms fruiting bodies (so-called truffles) in the soil; it is absolutely safe for humans, livestock, and other living organisms; it has strong antibacterial powers and can live in a variety of soils from polar to tropical regions around the world.

[0022] The white truffle, Tuber, is a mushroom fungus that grows naturally in alkaline soils (Figure 2) in Italy, France, and other countries. It belongs to the genus Tuber and family Tuberaceae. The other well-known fungus is the black truffle, but recent research has revealed that new species of the closely related genus Tuber have been discovered one after another in various parts of the world. Approximately 160 species have been discovered and registered from the northern to southern hemispheres, and it is said that approximately 15 species grow naturally in Japan.

[0023] In addition, to achieve this invention using the white truffle Tuber fungus and to implement it worldwide, a large amount of mycelium is required, but the inventors were the first in the world to succeed in mass-cultivating the white truffle Tuber fungus (Figure 8).

[0024] Furthermore, the present invention is not limited to white truffles, but can use most of the fungi in the genus Tuber, as well as 160 species of Tuber ssp., including black truffles and Chinese black truffles. Furthermore, since the closely related ascomycete wood-rotting fungi Pezizales. genus have similar characteristics (Patent Application No. 2019-164530), it is suggested that other ascomycete white wood-rotting fungi of the family Camphoraceae, Camphoraceae, and Camphoraceae that have the characteristics of "mycorrhizal fungi" can also be used. For example, among the fungi of the Camphoraceae family, it is thought that there is also the possibility of using the following fungi: Oo-chawandake, Beni-chawandake, Kuro-chawandake, Tanuki-chawandake, Kuriiro-chawandake, and False-churiiro-chawandake.

[0025] In nature, wood-rotting fungi are primary decomposers that survive by feeding on lignin, cellulose, and other physiologically active substances from dead leaves, fallen trees, and plant remains. There are more than tens of thousands of wood-rotting fungi, including basidiomycetes and ascomycetes, all over the world. However, there are only a few wood-rotting fungi, such as orchid fungi, matsutake fungi, and truffle fungi, that are wood-rotting fungi but also coexist with living plants and have the characteristics of a mycorrhizal fungus, helping the plants grow. In order to achieve the objective of the present invention, the wood-rotting fungi must be mycorrhizal fungi that coexist with living plants and help the plants grow. This is because if the fungi are not of this type, they cannot decompose and detoxify the pesticides remaining in living crops during cultivation. If the purpose is simply to decompose the residual pesticides in crop residues, basidiomycete wood-rotting fungi such as shiitake mushrooms that decompose plant remains can be used, but they cannot be used on living, growing crops.

[0026] White truffle is the most difficult mushroom to cultivate, but the fruiting body of this fungus is edible and is a harmless mushroom that has been enjoyed as the most expensive mushroom in the world. It has been eaten for hundreds of years, and there is no known evidence of its toxicity. The most important factor that attracted the inventor's attention to this white truffle Tuber fungus is that it is a safe and secure mushroom fungus. Furthermore, it has the rare characteristic of being a safe "mycorrhizal fungus" that does not harm crops, which is rare among wood-decaying fungi.

[0027] When the mycorrhizal fungus of the white truffle Tuber fungus grows symbiotically with the roots of a plant, the roots change into roots that do not have "root hairs." Figure 9 (1) shows the mycorrhizal roots of a Cattleya plant that grows symbiotically with an orchid fungus, and the roots do not have root hairs. On the other hand, the strawberry shown in Figure 9(2) has root hairs, but these root hairs are weak against drying and have a weak water absorption ability, so when the soil dries out, the plant is severely damaged. To avoid this risk, wild plants live in symbiosis with mycorrhizal fungi, using the mycorrhizal fungal hyphae as root hairs to withstand drying by utilizing the mycorrhizal fungal hyphae's strong water absorption ability. Also, white wood-rotting fungi such as the white truffle Tuber fungus break down the lignin and cellulosus in dead leaves and plant remains to produce glucose, which is then supplied to plants to complement their photosynthesis. At the same time, they also supply minerals, oxygen, and nitrogen to prevent continuous crop damage. In addition, many mycorrhizal fungi have strong antibacterial properties, putting disease-causing fungi into hibernation and protecting plants from disease. By living in symbiosis with mycorrhizal fungi, plants can store sufficient energy, which increases their "heat resistance" and "cold resistance," and perennial plants can maintain their health for many years. The 7,000-year-old Daio Cedar tree in Yakushima is said to be an example of this.

[0028] The following describes aspects of the present invention. [1] A method for decomposing and purifying residual harmful compounds in materials that contain or have residual harmful compounds attached thereto, using a white-rot ascomycete wood-rotting fungus having the characteristics of a mycorrhizal fungus. [2] The method for decomposing and purifying according to [1], wherein the ascomycete white wood-rotting fungus having the characteristics of a mycorrhizal fungus is a fungus selected from the family Camphoraceae, Camphoraceae, or Camphoraceae. [3] The method for decomposing and purifying according to [1], wherein the ascomycete white wood-rotting fungus having the characteristics of a mycorrhizal fungus is the white truffle Tuber fungus. [4] The method for decomposition and purification according to any one of [1] to [3], wherein the substance containing or having residual hazardous compounds attached thereto is a plant, an animal waste, a sewage sludge or a soil. [5] The method for decomposition and purification according to any one of [1] to [4], wherein the residual compound is a residual pesticide. [6] The method for decomposition and purification according to any one of [1] to [4], wherein the residual compound is an antibiotic contained in animal excrement. [7] A method for stable cultivation of plants using white-rot ascomycete fungi with mycorrhizal properties [8] The method for stably cultivating a plant according to [7], wherein the ascomycete white-rot fungus having characteristics of a mycorrhizal fungus is a fungus selected from the family Camphoraceae, Camphoraceae, or Camphoraceae. [9] The method for stably cultivating a plant according to [7], wherein the ascomycete white wood-decaying fungus having characteristics of a mycorrhizal fungus is the white truffle Tuber fungus.

[0029] Since the diluted suspension of white truffle Tuber fungus is in liquid form, it can be sprayed in the same way as ordinary pesticides, using watering cans, sprayers, airplanes, drones, etc. However, it is not limited to the diluted suspension, and it can also be used as a powder or granule that supports the white truffle Tuber fungus obtained by mass cultivation, so it can be selected according to the location and target object. In the case of herbicides, the amount used per area is small, so to decompose and purify them, it is sufficient to spray a suspension of white truffle Tuber fungus on the plant residues until they are sufficiently wet.

[0030] To break down residual pesticides in the crops during growth, spray 200 to 500 liters of a 30 to 50 times diluted suspension of white truffle Tuber fungus per 10 ares within 24 to 48 hours of pesticide application. In this case, add a wetting agent to a specified concentration and add 5g of white sugar per 1L of diluted solution. When white sugar is added, it inactivates pathogenic bacteria in the phyllosphere and rhizosphere, and the white truffle Tuber fungus fixes nitrogen in the air, making it possible to grow the truffle with reduced fertilizer and reduced or no pesticides. The effectiveness of both insecticides and fungicides becomes apparent about 24 hours after application, so it is important not to spray them any earlier than this.

[0031] To break down the herbicide components in weeds, in the case of stems and leaves, spray a diluted solution of the white truffle Tuber fungus suspension 24 to 48 hours after spraying the herbicide until the stems and leaves are sufficiently wet. In the case of decomposing residual pesticides in herbicide treatment residues (withered residues), even if the weeds are withered by spraying the herbicide, the herbicide components remain in the residues, and after the residues decompose, they remain and contaminate the soil, so it is best to spray the withered weed residues with a 30 to 50 times diluted solution of the white truffle Tuber fungus suspension until they are sufficiently wet. In addition, in the case of residues from weed cuttings, herbicide residues, or fruit tree cultivation residues, spray a 30 to 50 times diluted suspension of white truffle Tuber fungus until it is sufficiently wet (add a spreading agent and add 5 to 10g per 1L of diluted white sugar solution). Then, in about 7 to 15 days, the white truffle Tuber fungus will grow and reproduce on the residues, decomposing the residues while simultaneously decomposing and detoxifying pesticide and herbicide ingredients, putting soil disease bacteria to sleep, and purifying the residual nitrate nitrogen in the soil. Furthermore, the white truffle Tuber fungus will fix nitrogen in the air, allowing the field to be regenerated into one where reduced fertilizer and reduced or no pesticide cultivation is possible.

[0032] There are two possible ways to decompose pesticides remaining in the soil: <Method 1> How to grow white truffle Tuber fungus in soil in advance In early spring, or about 15 to 30 days before planting the crop, carefully spray 200 to 300 liters of a 30 to 50 times diluted suspension of white truffle Tuber fungus onto the soil surface (add 5 to 10 g per 1 liter of diluted white sugar solution) per 10 ares. It is important not to till the soil after spraying (tilling will cut the mycelium of the white truffle Tuber fungus and destroy the fungal network). In this way, the white truffle Tuber fungus grows on the surface of the ground, putting soil pathogens into hibernation. Even if pesticides or herbicides are sprayed afterwards, the white truffle Tuber fungus does not die, and it decomposes and detoxifies the pesticides that fall into the soil and the herbicides that have been sprayed, and by maintaining the soil in a constantly clean state, it prevents the crops from absorbing the pesticides through their roots. To explain this in Figure 3, in the left figure, the pesticides used in the previous crop remain not only in the crop but also in the soil, so by spraying a suspension of white truffle Tuber fungus there, the residual pesticides in the soil as well as the crop are reduced, as shown in the middle figure. If the next crop is grown in such soil, the residual pesticides in the next crop will also be very low, as shown in the right figure.

[0033] <Method 2> Spray white truffle Tuber fungus on the soil after weeds have grown. After the weeds have started to grow, spray a 30 to 50 times diluted suspension of white truffle Tuber fungus until it is thoroughly wet (add a wetting agent, 5 to 10 g per 1 L of diluted white sugar). Spray the pesticide or herbicide 2-3 days after spraying. This will allow the white truffle Tuber fungus to decompose and detoxify the pesticide or herbicide ingredients.

[0034] In addition, the white truffle fungus Tuber can grow and reproduce at temperatures between 0 and 5°C and even under snow, so if an herbicide is sprayed in the fall, the soil bacteria may go dormant at the low temperatures and not decompose the herbicide. In late autumn, spraying 200 to 300 liters of a 50-fold diluted suspension of white truffle Tuber fungus (with added white sugar) per 10 ares will break down the residual herbicide components and put the pathogenic fungus into dormancy.

[0035] As described later, the white truffle Tuber fungus has the ability to decompose toxic substances such as fungicides and insecticides, so fungicides and insecticides are sprayed using a spraying device like the one shown in Figure 7 (an example of a cherry orchard). If the same spraying device is used to spray a suspension of the white truffle Tuber fungus 1-2 days after the fungicide or insecticide has taken effect, the fungicide or insecticide that has completed its function will be decomposed, and the amount of residual fungicide or insecticide can be significantly reduced.

[0036] Sewage residue contains high concentrations of nitrate nitrogen and bacteria that do not live in symbiosis with crops. It is known that crops do not grow well if this residue is applied directly to farm fields, so it is often incinerated. The sewage residue is flattened to a height of 15 to 20 cm, and a suspension of the white truffle Tuber fungus is added to it and kept at a humidity of about 80%. The white truffle Tuber fungus will grow to saturation and be complete in about 7 to 15 days at a temperature range of 0°C to 50°C. White truffle Tuber fungus is a mycorrhizal fungus that helps crops, and has the ability to put soil disease-causing bacteria into hibernation. It can suppress soil diseases and transform soil into an excellent soil conditioner for reduced fertilizer and reduced or no pesticide cultivation, making it possible to establish a circular agriculture system.

[0037] Figure 4 (1) shows the residuals after the harvest of cabbage, and Figure 4 (2) shows the residuals after the harvest of corn. In particular, the pesticide components contained in the residues of crops harvested in the fall remain in the soil as the temperature drops toward the cold season in temperate regions, and bacteria and microorganisms lose their activity and are unable to decompose the residues. In addition to residual pesticides, disease-causing bacteria also live in the crop residues after the harvest. To remove such residual pesticides from crop residues, the crop residues after harvest are spread on the ground and sprayed with a diluted 50 to 100 times suspension of white truffle Tuber fungus (add 5 to 10g to 1L of diluted white sugar solution). This puts the pathogenic fungus in the crop residue into dormancy and prevents the disease in the next crop. The residual pesticides contained in the crop residue can be decomposed, and the crop residue can be used as a raw material for the carbon cycle in the field.

[0038] A wide variety of processed foods are produced from a wide variety of crops, but most of these raw materials contain residual pesticides. In order to break down the residual pesticides from such raw materials, as shown in Figure 5, the raw materials are immersed in a diluted suspension of white truffle Tuber fungus for 1 to 2 hours during the food processing process, and then a process of washing with water is inserted. This type of treatment makes it possible to break down, detoxify, and purify almost all of the residual pesticides in the raw materials.

[0039] In tea cultivation fields, large amounts of pesticides (fungicides, insecticides) are sprayed (usually about eight times) (Figure 6 (1)), and as of 2020, approximately 100 types of pesticides for tea are registered and in use. Naturally, processed "tea" contains "residual pesticides," although the amount is below the standard level, and when the tea is drunk, these residual pesticide components naturally leach out along with the components contained in the tea leaves. In addition, when making tea, tea from multiple producers is blended, so it is nearly impossible to identify the "tea farm" when analyzing the finished tea. This problem can be solved by adding a process for decomposing and detoxifying residual pesticides to the processing process, just like processed foods. That is, as shown in Figure 6 (4), before picking and steaming the tea leaves, they can be purified by soaking them in a diluted suspension of white truffle Tuber fungus for one to several hours, and then washed with water before the "steaming" process. Figure 6 (3) shows the conventional tea manufacturing process.

[0040] In order for the white truffle Tuber fungus to grow, it needs a carbon source as an energy source. If white sugar is added as this carbon source, the white truffle Tuber fungus will grow and grow rapidly using this as food. To be specific, a solution containing 10g of sugar dissolved in 1 liter should be sprayed once a month at a rate of about 200 liters per 10 ares.

[0041] The difference between fungicides and insecticides is that insecticides do not have a significant effect on the white truffle Tuber fungus. In addition, the white truffle Tuber fungus does not break down the insecticide components within 24 hours of spraying. Taking advantage of this characteristic, insecticides can be mixed with white truffle Tuber fungus suspensions and sprayed. Most insecticides have an insecticidal effect within 24 hours of spraying, so it is desirable to decompose, detoxify, and purify the residual pesticides as soon as possible after that, but by mixing them, it is possible to kill insects with the insecticide and then decompose the remaining insecticide in a single spray.

[0042] In this invention, we have newly discovered that the white truffle Tuber fungus produces the plant hormone indole-3-acetic acid. The discovery of this hormone production allows us to produce safe and secure food, and at the same time, by mixing this hormone into a suspension of the white truffle Tuber fungus and spraying it, it will decompose residual pesticides during growth and at the same time promote growth, enabling stable production that can withstand climate change, which is expected to increase production. Effect of the Invention

[0043] The effects of the present invention are listed below. 1. The decomposition and detoxification effect of the white truffle Tuber fungus on residual pesticides allows the production and supply of safe, secure food with fewer residual pesticide components. Because the white truffle Tuber fungus is resistant to pesticides (fungicides, insecticides, herbicides), it can decompose a variety of pesticides even while the crops are growing. By spraying a diluted suspension of the white truffle Tuber fungus on crops before harvesting, it is possible to produce and supply safer agricultural products with fewer residual pesticides, which is what consumers desire. This will allow for both stable food security and safe food, making it possible to secure food to meet the future population growth. 2. Residual pesticides that adhere to the inside and outside of crops due to the spraying of fungicides, insecticides, and herbicides during crop growth can be decomposed and detoxified by spraying a 30-fold diluted suspension of white truffle Tuber fungus on the leaves of the aboveground parts 12 to 48 hours after the spraying. This not only enables safe and secure production of food and fresh vegetables, but also prevents pesticide damage to the growers' bodies, prevents damage to flower-visiting insects such as honeybees, and preserves the ecosystem. In addition, the rapid decomposition of herbicides contributes to the conservation of the global environment in soil, rivers, and seas. 3. In addition to decomposing residual pesticides, the suspension of white truffle Tuber fungus contains the plant hormone "indole 3. acetic acid" produced by the white truffle Tuber fungus, which decomposes residual pesticides and promotes crop growth, making it possible to reduce fertilizer and increase harvests. 4. By soaking various processed food ingredients in a diluted suspension of white truffle Tuber for a certain period of time, it is possible to decompose and purify the residual pesticides contained in the processed ingredients. This makes it possible to produce safe and secure processed foods, beverages, teas, and herbal medicines. 5. By quickly breaking down and detoxifying the pesticide components after controlling disease bacteria, pests, and weeds without reducing the effectiveness of the pesticide, it is possible to cultivate crops safely and securely with stable harvests, and the soil can also be purified. 6. White truffle Tuber has antibacterial properties and the ability to fix nitrogen in the air. By spraying a suspension of white truffle Tuber on crops before and during cultivation, the Tuber fungus will decompose and detoxify residual pesticides in the crops' bodies and in the soil, and at the same time, the white truffle Tuber fungus will live in the soil, allowing for reduced fertilizer and pesticide use in cultivation. 7. The method of the present invention is not limited to the place of application. By spraying the white truffle Tuber suspension 24 to 48 hours after spraying herbicides in areas other than agricultural land, it not only neutralizes the residual herbicide components, but also decomposes dead weeds, and prevents the human body from coming into contact with residual pesticides, making these areas safer. 8. This method can be implemented in fields in tropical and tropical regions around the world, and even in acidic and alkaline soils, so it can prevent the degradation of fields and thereby ensure both food safety and food security. 9. Purifying and regenerating the nitrate nitrogen in the current farm soil will restore the nutrient balance of the field, making it possible to grow healthy crops. By permanently preserving this clean soil, the disappearance of tropical rainforests can be prevented. Furthermore, nitrate nitrogen remaining in the soil is reduced to nitric oxide gas, which causes global warming, but purifying the soil with the white truffle Tuber fungus, which has the ability to fix atmospheric nitrogen, can solve the problem of greenhouse gas emissions. 10. White truffle Tuber is a mycorrhizal fungus that can compensate for the lack of photosynthesis in crops caused by global warming, stabilizing crop growth and making the crops more resistant to pests and diseases. This not only enables safe and stable food production, but also stable and sustainable agricultural management. 11. The white truffle Tuber fungus is harmless to humans, livestock and other living organisms. It can survive and reproduce in almost all areas of the world, making it possible to neutralize residual pesticides in farm and non-farmland all over the world, and preventing the destruction of ecosystems caused by residual pesticides. 12. Food and the Ecosystem By decomposing and detoxifying residual pesticide components, we can prevent the decline in the immune system of humans and other living organisms. By suppressing the decline in immunity, which has emerged as one of the most important issues for global society in the future, we can make the human body, living organisms, medical care, and society more resistant to major epidemics. 13. By decomposing and detoxifying the antibiotics contained in livestock waste and using the resulting culture soil to cultivate crops that do not contain antibiotics, it is possible to maintain the human body in a state where the effects of antibiotics can be sustained with food that does not contain antibiotics. 14. Methane gas power generation using livestock waste is gaining attention worldwide and is becoming more widespread, but the antibiotics contained in the gas inhibit the growth and reproduction of methane bacteria, and methane gas cannot be produced in many cases. This problem can be solved by breaking down the antibiotics using white truffle Tuber fungus before putting livestock waste into the methane gas generation tank. 15. In crops and fields where it is difficult to grow completely pesticide-free, by combining the use of white truffle Tuber fungus with the conventional use of pesticides, it is possible to produce food that is almost as safe as pesticide-free cultivation, because even if pesticides are sprayed, the sprayed pesticides are decomposed and detoxified after the effect is exerted. 16. Decomposition and purification of pesticides can be achieved by mixing the pesticide with a suspension of white truffle Tuber fungus and spraying it, which makes it possible to kill insects and decompose residual pesticides in one operation. 17. In the post-COVID-19 society, where we coexist with the virus, the keyword will be "immunity." However, food containing residual pesticides is said to be one of the factors that weakens the "immunity" of humanity. By decomposing and purifying residual pesticides using this invention, it is possible to prevent the weakening of the human immune system. [Brief description of the drawings]

[0044] [Figure 1] Acid soil fields around the world [Diagram 2]Alkaline soil fields around the world [Diagram 3] Previous crop residual pesticide, next crop residual pesticide conceptual diagram [Figure 4] Crop residue images [Diagram 5] Schematic diagram of the processed food manufacturing process [Figure 6] (1) Spraying pesticides on tea fields (2) Soaking stacked tea leaves in a suspension of white truffle fungus (3) (4) Tea manufacturing process [Figure 7] Decomposition and detoxification test of pesticides (fungicides and insecticides) by spraying a 50-fold diluted suspension of "white truffle" Tuber fungus on the leaves of cherries [Figure 8] Mass cultivation of the white truffle fungus Tuber [Figure 9] Image of mycorrhizal symbiosis with white truffle mycorrhizal fungi [Figure 10] Effects of white truffle fungus on seedlings [Figure 11] Cell permeability test of white truffle fungus [Figure 12] Resistance test of white truffle fungus to fungicides [Figure 13] Microbial antibacterial test of the white truffle fungus Tuber. [Figure 14] Inactivation test of alcohol fermentation yeast by white truffle fungus Tuber. [Figure 15] Neonicotinoid decomposition and detoxification test of white truffle fungus [Figure 16] White truffle fungus decomposition and purification test for various herbicide residual pesticides [Figure 17] Round-up resistance test of the white truffle Tuber fungus [Figure 18] Time course of decomposition of roundup by the white truffle fungus Tuber [Figure 19] Livestock waste decomposition test using white truffle Tuber fungus [Figure 20] White truffle Tuber cultivation of strawberries outdoors with reduced pesticides [Figure 21] Atmospheric nitrogen fixation test of the white truffle Tuber [Figure 22] Organic matter decomposition test of white truffle Tuber fungus [Diagram 23] White truffle Tuber soil growth test [Figure 24] Growth test of white truffle Tuber in highly acidic soil [Diagram 25] Growth test of white truffle Tuber in strong alkaline soil [Figure 26] Low-temperature growth test of the white truffle Tuber fungus [Figure 27] High temperature growth and reproduction test of the white truffle Tuber fungus [Figure 28] Growth and reproduction test of the white truffle Tuber fungus in diluted insecticide solutions [Figure 29] Decomposition and detoxification of various insecticides by the white truffle Tuber fungus. Insecticidal effect test using the detoxified solution. [Diagram 30] A study on the decomposition and purification of nitrate nitrogen contained in sewage purification residues using the white truffle Tuber fungus [Diagram 31] A trial of cultivating strawberries in elevated beds and Chinese cabbage in the field using sewage residue purified by the white truffle Tuber fungus [Diagram 32] Testing the effect of foliar spraying of white truffle Tuber fungus culture suspension on plant and crop growth (testing plant hormone production) [Diagram 33] Growth test of rice sprayed with a suspension of cultured white truffle Tuber fungus (Plant hormone production test 2) [Diagram 34] Detoxification and decomposition test of grass endophytes by spraying a suspension of white truffle Tuber fungus culture [Diagram 35] Testing the penetration time of a 30-fold diluted suspension of the white truffle Tuber fungus culture sprayed on crops and plant tissues [Diagram 36] White truffle Tuber suspension 30-fold diluted solution skin penetration test DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0045] Examples of the present invention will be described below, but the present invention is not limited thereto. EXAMPLES

[0046] Test 1 <Liquid culture test of white truffle Tuber mycelium> Cultivating white truffles is one of the most difficult mushroom cultivations, along with that of matsutake, and there are no successful examples anywhere in the world of mass cultivation of fruiting bodies or mycelium. Figure 8(1) shows the lysed mycelium of white truffle fruiting bodies taken out on a basic white truffle medium. Figure 8 (2) shows an isolated colony (mycobion) that was cultured from the lysed mycelium of (1). Figure 8 (3) shows the mycelium harvested from the isolated colony in (2) and mass-cultured. [Composition and culture conditions of the white truffle Tuber basal medium] water 1000cc Hyponex 3g (liquid fertilizer, manufactured by Hyponex Japan Co., Ltd.) 30g white sugar Minimum temperature 5℃ Maximum 15℃ Stationary, light culture. As shown in the above photo, the mycelium of the white truffle fruiting body was cultured in the above basal medium to form colonies, and the white truffle fungus Tuber was mass-cultured from the resulting colonies.

[0047] Test 2: Comparison of biofilm production in liquid culture of Tuber. and Tuber. root nodule bacteria Rhizobium sp. Figure 8 (4) shows the shape and production images of the biofilms in the case of the white truffle Tuber monoculture (right) and in the case of co-culture with Rhizobium sp. (left) in the liquid medium of the basic culture medium for the white truffle Tuber. Figure 8 (5) shows a biofilm formed by co-cultivation of the white truffle fungus Tuber. and the root nodule bacteria Rhizobium sp. (Enlarged photo) Figure 8 (6) is an enlarged image of a biofilm formed in a monoculture of the white truffle fungus Tuber. As the photo shows, although it is inferior to the bacterial flora formed by the symbiotic relationship between the white truffle fungus Tuber. and the root nodule bacterium Rhizobium sp. (a commercially available root nodule bacterium), a sufficient amount of biofilm can be formed even with the white truffle fungus Tuber. alone. The biofilm formed in this way was crushed in a mixer and diluted with 100 parts water to prepare a 100-fold suspension of the white truffle fungus Tuber, which was used in various experiments. As is clear from this test, the white truffle fungus Tuber grows well in liquid culture. In order to implement the present invention on a global scale, it is necessary to mass-produce the white truffle fungus Tuber., and it is advantageous to be able to easily produce a biofilm in liquid culture.

[0048] Test 3: Experiment to confirm that the white truffle fungus Tuber. does not harm plants and makes them healthy A suspension of the white truffle fungus Tuber. was sprayed onto seedlings of cabbage (left), Chinese cabbage (right) (Fig. 10(1)) and mycorrhizal sea daisy (Fig. 10(2)). Photographs taken 5 days later are shown in Fig. 10(3) and Fig. 10(4). None of the seedlings were affected by the white truffle fungus Tuber. This suggests that the white truffle fungus Tuber. has mycorrhizal function.

[0049] Test 4: Experiment to confirm that the white truffle fungus Tuber. rapidly penetrates plant and animal cells When a suspension of the white truffle fungus Tuber. was applied to inflamed skin caused by a mosquito bite (Fig. 11(1)), the inflammation healed within a few minutes (Fig. 11(2)). This indicates that the white truffle fungus Tuber. or its secretions quickly penetrated the skin cells and decomposed the toxic components.

[0050] Test 5 <Test on the resistance of white truffle fungus Tuber to fungicides> Benlate (Figure 12(1)) and procymidone (Figure 12(2)) were diluted at the normal dilution ratio and sprayed on the white truffle fungus Tuber cultivated in a beaker. The photographs show the state of the fungus several days after spraying, and the white truffle fungus Tuber was not affected by either fungicide.

[0051] Test 6 <Microbial antibacterial test of white truffle Tuber.> After the preparation of the white truffle Tuber culture medium, it was left in an unsterilized, open room. An airborne microbial proliferation test was conducted at a minimum culture temperature of 15°C and a maximum of 25°C. In Figure 13(1), 10cc of white truffle mycelium crushed finely in a mortar was added to 100cc of water to create a suspension (non-sterile solution), which was then added to the above non-sterile culture medium. The photo shows the state after 7 days, when airborne microbial spores should be constantly falling onto the culture medium, but no microbial colonies have formed at all. It can be assumed that the white truffle fungus inactivates the fallen spores of airborne microorganisms, preventing them from germinating. Figure 13(2) shows the state on the 7th day after inoculating the above non-sterile culture medium with "Aspergillus niger" to form colonies, and then adding the above white truffle suspension to culture. Just as the white truffle fungus feeds on the black mold colonies, it extends its hyphae over the colonies and exerts its dominance over them. Figure 13 (3) shows the state on the 7th day after inoculating the "Peniculum" in the same way as (2) to form colonies, then adding the above-mentioned white truffle fungus suspension and culturing. Penicillium difficile produces the antibiotic penicillin and creates an inhibition circle to prevent other bacteria from invading an area. However, the white truffle fungus neutralizes this inhibition mechanism of the mold, dissolves the mold and uses it as food, forming a white truffle flora on top of the mold flora.

[0052] Test 7 <Inactivation test of alcohol fermentation yeast using white truffle fungus Tuber.> The white truffle fungus Tuber. was aseptically inoculated onto a sterilized culture medium of the white truffle base medium, and alcohol fermentation yeast (yeast derived from sake lees) was inoculated in parallel to the side of the medium. Cultivation temperature: 25°C. Cultivated in a bright place. The photo (Figure 14) shows the state after 10 days of treatment and cultivation. The strong antibacterial ability of the white truffle fungus Tuber. inhibited the growth of the alcohol fermentation yeast, which was cultivated at the optimal temperature. Although colonies were formed, growth thereafter stopped. This test demonstrated that the white truffle fungus Tuber. has the ability to inactivate yeast fungi that ferment sugar into alcohol, proving that it is possible to administer sugar as a carbon source to establish and settle the white truffle fungus and rhizobia in the field. This is because if sugar is added to soil with active alcohol-producing yeast, the sugar will be converted into alcohol, damaging the white truffle fungus Tuber. and causing severe damage to the roots of the crops.

[0053] Test 8 <Experiment on the decomposition and detoxification of the insecticide neonicotinoid by the white truffle fungus Tuber.> The pesticide that is causing the most problems in the world due to pesticide residues in biological ecosystems is "neonicotinoids." This pesticide has been used to control many pests around the world because of its excellent residual activity. However, because the residual components of this pesticide have a significant impact on the ecosystems of various insects, especially honeybees, its use is being banned in many countries around the world. Based on the above, we conducted a decomposition and detoxification test of residual pesticides by spraying a diluted suspension of the neonicotinoid pesticide "nitenpyram" in "white truffle" Tuber fungus. [Test Method] Test material: 2 trays of mycorrhizal sea daisy. Pesticide name: Nitenpyram 100 times diluted solution Pesticide residue decomposition detoxifier White truffle Tuber bacteria suspension 100x solution Number of tests conducted: 2 (Tests A and B) Spray two trays of mycorrhizal sea daisies with a 1000-fold diluted solution of nitenpyram. [Test A] B. Untreated area: Nothing done (b) Treatment area: 24 hours after the application of nitenpyram, a suspension of the white truffle Tuber fungus was sprayed in the same manner as the insecticide (Figure 15(1)). [Sample collection and analysis] Stems and leaves of mycorrhizal sea daisy were collected from the trays of the untreated and treated areas, one day after the untreated area and six days after the treated area, and the samples were crushed in a mortar and pestle. 1 g of the sample was mixed with 99 cc of honey to create 100 g of sample solution (Figure 15 (2)). This was used as a sample to analyze the residual pesticide concentration of nitenpyram 24 hours after spraying and 5 days after spraying a suspension of white truffle Tuber fungus. [Test B] In Test A, the two trays of the untreated area of ​​the mycorrhizal sea chrysanthemum and the treated area of ​​the white truffle Tuber fungus were sprayed again with a 1000-fold diluted solution of nitenpyram. B. Untreated area: Nothing done B. Treatment area: 24 hours after the second Nitenpyram spray, the white truffle Tuber suspension was sprayed. [Sample collection and analysis] Five days after treatment with the white truffle Tuber fungus, the stems and leaves of the mycorrhizal sea daisy were collected from both the untreated and treated areas and crushed. The Mie Environmental Conservation Corporation then analyzed the residual pesticide concentrations using the same procedures as in Test A. The results are shown in Table 2. Of the 10 types of pesticides tested, the following two were detected, and it can be seen that the amount of pesticide residues was dramatically reduced by spraying the suspension of white truffle Tuber.

[0054] [Table 2]

[0055] Test 9: <Decomposition and detoxification test of pesticide components by immersing tea leaves sprayed with pesticides in a suspension of white truffle Tuber fungus> Sagae tea leaves A and B were soaked in a suspension of white truffle Tuber for 2 hours during the tea manufacturing process according to the process in Figure 16-2. The Mie Environmental Conservation Corporation analyzed the residual pesticides in the finished tea leaves. As shown in the table below, the following 5 types of pesticides were detected out of 120 types of pesticides tested, but all were below the standard value.

[0056] [Table 3]

[0057] Test 10: Decomposition and detoxification test of fungicides and insecticides by white truffle Tuber fungus in cherry cultivation Cherries grown in greenhouses are sprayed with fungicides and insecticides using equipment such as that shown in Figure 7. Therefore, 24 to 48 hours after the fungicides and insecticides had been sprayed and their effects had been felt, a 50-fold diluted suspension of the white truffle Tuber fungus was sprayed using the same equipment, and the residual pesticides in the harvested cherries from Higashine were examined. Analysis was conducted at the Tsukuba Analytical Center, and the following three types of pesticides were detected out of the 250 types tested, all of which were below the standard values.

[0058] [Table 4]

[0059] Test 11: <Soil decomposition and purification test of various herbicide residues using white truffle Tuber fungus> Currently, agriculture around the world is facing severe weed overgrowth due to global warming, and agriculture is becoming dependent on herbicides, which is causing serious problems with the carcinogenicity of herbicides. One of the objectives of this invention is to use the white truffle Tuber fungus to decompose and detoxify the residual herbicides in the soil, which is currently a problem in many countries around the world. By purifying the soil, the inside of the crops can also be purified, making it possible to produce small quantities of crops safely and securely. We conducted a verification test to verify the possibility of decomposing herbicide components using the white truffle Tuber fungus. [Test materials] Small granular Akadama soil was placed in No. 3 and No. 5 pots, and diluted solutions of the standard spraying concentrations of each herbicide were thoroughly irrigated to prepare test soil. A 100-fold diluted suspension of "white truffle" Tuber fungus was sprayed onto the surface using a watering can, and the surface was left as is for 10 days. After 10 days, cabbage seeds were sown in each post. In Figure 16(1), the herbicides sprayed onto each post were as follows: 1:MCPP Ward 2: Casoron District 3: Land Up Area 4: Busta Ward 5:2,4-D Ward 6: MCPP irrigation and white truffle Tuber suspension treatment area 7: Laputiuron District 8: Kusatoroze District As is clear from Figure 16 (2), germinated in all posts except for the untreated area 6 where the 100-fold diluted white truffle Tuber suspension was not sprayed. In this way, the white truffle Tuber can decompose and detoxify the above-mentioned multiple herbicides.

[0060] Test 12 <Roundup resistance test using white truffle Tuber fungus> Roundup is a herbicide (a type of pesticide) developed by the American company Monsanto in 1970. Its active ingredient is glyphosate isopropylamine salt (a compound with a phosphonomethyl group substituted on the nitrogen atom of glycine). This herbicide is a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibitor that inhibits the synthesis of 5-enolpyruvylshikimate-3-phosphate in the plant body, and thereby inhibits the synthesis of aromatic amino acids (tryptophan, phenylalanine, tyrosine) and proteins and metabolic products containing these amino acids.It is an absorbed and translocated herbicide that causes the entire plant it comes into contact with to die (to stems and leaves), and is a non-selective herbicide that causes damage to most plants. A bacterium that does not have the shikimic acid cycle was discovered, and by introducing the gene of this bacterium into crops, crops that do not die from round-up were created, and round-up agriculture became widespread worldwide. As a result, the residue of this herbicide in food has become a major problem. If the white truffle Tuber fungus is a fungus that dies from Round Up, not only will it not be possible to use the white truffle Tuber fungus to decompose the residual components of Round Up, but the fungus itself will die. For this reason, this test was carried out. [Test Method] Hyponex basal medium After autoclaving and cooling, 5cc of a 50x solution of Runup was added and the Runup solution was mulched onto the culture medium. Two pieces were made. 1 cc of a suspension of white truffle Tuber fungus was added to this culture medium and cultured at a minimum temperature of 5°C and a maximum of 20°C in a bright room. Figure 17(1) shows the state of the white truffle Tuber colonies formed on the wrap-up medium about three months after treatment. Figure 17(2) is a photograph taken from above. White truffle Tuber fungus grew and multiplied in the Hyponex medium supplemented with Landup. The fact that it did not die but instead grew proved that the white truffle Tuber fungus does not use the "shikimic acid cycle" for protein synthesis.

[0061] Test 13: Optimal application time test for decomposition of herbicide residual pesticides using a suspension of white truffle Tuber fungus This invention involves spraying a suspension of "white truffle" Tuber fungus after spraying herbicide, which decomposes and detoxifies residual components in the soil and ecosystem, purifying the environment. Most of the herbicides currently in use act on the physiological activity of plants, making them unable to survive and causing them to die. When the herbicide sprayed on the leaves is absorbed by the cells, migrates to the entire plant tissue, and acts on the physiological activity production function, the enzyme group of "white truffle" Tuber fungus breaks down the herbicide components in the body, and can break down the residual components of fungicides and insecticides in the body. Therefore, a test was conducted to determine how many hours after the herbicide application a suspension of "white truffle" Tuber fungus should be sprayed to most effectively break down the pesticide components. [Test Method] Landup Maxload was used as the pesticide, and a solution containing 100cc of Landup added per 1000cc of soil (a solution 10 times more concentrated than normal use) was sprayed onto the leaves of 14 pots of mycorrhizal sea daisies. A 100-fold diluted suspension of "white truffle" Tuber fungus was sprayed onto each of the two pots at the following times: A: no treatment, B: 4 hours later, C: 6 hours later, D: 12 hours later, E: 24 hours later, F: 60 hours later. The condition of the mycorrhizal sea daisy was then observed. Figure 18 shows the state of mycorrhizal sea daisies one month later, with two pots each from the left in areas A, B, C, D, E, and F. As is clear from Figure Test 6, after spraying Landup, the two pots sprayed with the "White Truffle" Tuber fungus suspension at the untreated area, 4 hours, and 6 hours later were unable to decompose and detoxify the residual components of Landup in the plants' bodies, and both died 30 days after treatment. In the 12-hour treatment area, one pot died and the other survived, and in the 24-hour, 48-hour, 60-hour, and 72-hour treatment areas, the "White Truffle" Tuber fungus decomposed and detoxified the residual components that had penetrated into the plants' bodies, allowing the mycorrhizal sea daisies to survive. This test demonstrated that spraying a 100-fold diluted suspension of "white truffle" Tuber fungus on the leaves between 24 and 60 hours after the application of the pesticide decomposed and detoxified the pesticide components remaining in the crop.

[0062] Test 14 <Test on resistance of white truffle Tuber to antibiotic residues in livestock waste> Antibiotics from feed remain in livestock waste. Anaerobic bacteria are used to produce compost and culture soil from this material, which means that antibiotics are hardly broken down. As a result, compost and culture soil contain antibiotics. When this is applied to fields, it is absorbed by crops and remains in the crops. When people eat this, it accumulates in the human body, and when they become sick, there are cases where antibiotics are ineffective. To prevent this, it is desirable to quickly decompose livestock waste using white truffle Tuber fungus to decompose and detoxify the antibiotics. Antibiotics are mainly produced by bacteria such as actinomycetes to protect the living space from other microorganisms. Biologically active substances produced in nature are usually decomposed and detoxified by wood-rotting fungi. Nature has a system in place whereby anything produced by living organisms is decomposed by nature, and antibiotics are no exception. It is highly likely that the antibiotics contained in the lignin and cellulose of the plant fiber excreted by the white truffle Tuber fungus are simultaneously decomposed and detoxified by decomposing the lignin and cellulose. Test material: Dairy cow manure (moisture content: about 70 to 80%) Test method: 2 kg of cow dung was flattened to a thickness of 3 cm and the white truffle Tuber fungus was planted on it. Spray 30x suspension and cover with vinyl. Minimum 8℃ Maximum 20℃ Indoor culture Photograph taken 10 days after treatment (Figure 19) As is clear from Figure 19, the white truffle Tuber fungus can grow in dairy cow manure containing antibiotics and has resistance to antibiotics. In addition, based on the above-mentioned laws of nature, it is believed that the white truffle Tuber fungus decomposes not only cellulose and lignin but also antibiotics.

[0063] Test 15: Experiment to reduce the use of pesticides in outdoor strawberry cultivation by spraying diluted suspension of white truffle Tuber fungus. This invention is a technology that enables safe and secure food production by decomposing and detoxifying residual pesticide components in the body of crops during crop cultivation by spraying a diluted suspension of white truffle Tuber fungus. At the same time, we tested whether it is possible to suppress the occurrence of disease by inactivating (dormant) crop disease bacteria with the strong antibacterial action of white truffle Tuber fungus, thereby significantly reducing the number of times and the amount of pesticide spraying. This experiment involved reduced pesticide cultivation using strawberries, which are the crop that receives the most pesticide sprays and the highest amounts of pesticide used (approximately 50 to 70 sprays) among all crops in the world. Test material: Outdoor strawberry, Takakou Wase, two-year-old outdoor field (Fig. 20(1), Fig. 20(2) (enlarged view)) Test method: A 50-fold diluted suspension of the white truffle Tuber fungus was sprayed on the leaves 10 times at 10-day intervals from April 1. After three months, strawberries were able to bear fruit well, despite the absence of any pesticides (Figure 20(3)).

[0064] Test 16: Nitrogen fixation by the white truffle Tuber fungus. In this test, the white truffle Tuber fungus obtained in Test 1 was grown in the following minimal nitrogen medium. Potassium phosphate monobasic 3g Wako Pure Chemical Reagent Grade 1 Water 1000cc Purified water Glucose 30g Wako Pure Chemical Reagent Grade 1 Agar 15g Wako Pure Chemical Reagent Grade 1 [Test Method] After cooling in the autoclave, the white truffle Tuber fungus was inoculated and cultured in a bright room with the following culture conditions: minimum temperature 5℃, maximum temperature 18℃. Wako Pure Chemical Reagent Grade 1 contains a very small amount of nitrogen, but the white truffle Tuber fungus grew and formed colonies in this very small amount of nitrogen medium (Figure 21). This demonstrated that the white truffle Tuber fungus, a mycorrhizal fungus and ascomycete white wood-rotting fungus, can fix nitrogen on its own.

[0065] Test 17 <Organic matter decomposition test using the white truffle fungus Tuber.> The photograph in Figure 22(1) shows weeds that were cut, placed in a storage jar, sprayed with a suspension of white truffle Tuber ssp., and incubated at 25°C. The photograph shows the state of decomposition five days after treatment. The photograph in Figure 22(2) shows pellets made from organic industrial waste cardboard and old paper placed in a 300cc flask, sprayed with a suspension of the white truffle fungus Tuber, and then cultivated at 25°C. The photograph shows the state 20 days after treatment. The white truffle fungus Tuber. is an ascomycete white wood-decay fungus and is the only fungus on Earth that can decompose lignin from organic industrial waste. This test suggested that the white truffle fungus Tuber. has the ability to decompose lignin.

[0066] Test 18 <Soil growth test of white truffle fungus Tuber.> A suspension of white truffle bacteria and general-purpose root nodule bacteria was added to the field soil at a concentration of 1 m. 2 per 1m 2 After spraying 500cc of water and then a sugar solution (1000cc of water and 10g of white sugar), after 25 days, the white truffle fungus Tuber began to grow and multiply in the soil, as shown in the photo in Figure 23. The white truffle fungus Tuber. is a mycorrhizal fungus that lives symbiotically with trees, including hard-leaved broad-leaved trees such as oaks. It was previously thought that the fungus could not live and grow without the roots of the trees with which it mutually benefits. However, the results of this test suggest that it grows well in soil that contains a nitrogen source, mineral source, temperature, moisture, and oxygen.

[0067] Test 19 <Growth test of the white truffle fungus Tuber. under strong acidic conditions> Figure 24(1) shows a colony (bacterial flora) of the white truffle fungus Tuber. cultivated in a basic medium. [White truffle Tuber medium composition and culture conditions] water 1000cc Hyponex 3g 30g sugar 15g agar Minimum temperature 5℃ Maximum 15℃ Stationary, light culture. Figure 24(2) shows the growth and reproduction of the white truffle Tuber. after 7 days of cultivation, after adding 5cc of pyruvic acid solution at pH 3.5 to the colony-forming culture medium made of the basic medium of the white truffle Tuber. Acidic soil occupies a large area of ​​agricultural fields around the world (Figure 1), but the white truffle fungus Tuber. grows and reproduces well even in such fields, suggesting that it can constantly produce mycelial chitin, which is food for actinomycetes.

[0068] Test 20 <Growth test of the white truffle fungus Tuber. under strongly alkaline conditions> Figure 25(1) shows colonies of the white truffle fungus Tuber. growing on a basic medium. Figure 25 (2) shows the flask in the state shown in photo (1) after adding quicklime granules (culture temperature 25°C). The quicklime in the flask now has a strong alkaline pH of 11. Figure 25(3) shows the state 7 days after adding the quicklime granules. Surprisingly, the mycelium of the white truffle fungus Tuber. grew even on the quicklime granules (culture temperature 25°C). White truffles originate from Europe and China, which have limestone soils, so it was expected that they would be able to grow in highly alkaline soil, and we were able to confirm this.

[0069] Test 21 <Growth test of the white truffle fungus Tuber. under low temperature and snow conditions> Figure 26(1) shows the state in which the sterilized Tuber. basic medium was inoculated with the white truffle fungus and the culture flask was buried in snow. The temperature in the snow was 0 to 5 degrees Celsius. Figure 26(2) shows the state 7 days after treatment, and the photo on the upper right is an enlarged image. As shown in the photo, huge colonies were formed. This test suggests that the white truffle fungus can grow even during the winter in both the cold and temperate regions of the world.

[0070] Test 22 <Growth and reproduction test of the white truffle fungus Tuber. under high temperature conditions> (38) Figure 27(1) shows the state in which the white truffle Tuber. was inoculated into the white truffle basal medium and the culture flask was placed on a shelf in the greenhouse. It was covered with transparent vinyl, and the flask temperature was set to a maximum of 50°C during the day and a minimum of 25°C in the morning. Figure 27(2) shows the growth of the white truffle fungus Tuber in the flask after 10 days. The high temperature had no effect on the growth and reproduction rate, which was the same as at the optimum temperature of 22°C. This test demonstrated that the white truffle fungus Tuber can grow in tropical and temperate summers around the world without being affected by high temperatures.

[0071] Test 23 <Growth and reproduction test of white truffle Tuber fungus in 1000-fold diluted solutions of various insecticides> White truffle Tuber fungus was added to 1000-fold diluted solutions of various insecticides, and cultured at a minimum temperature of 10°C and a maximum temperature of 25°C in a bright room. Figure 28(1) shows the growth of the white truffle Tuber fungus after 7 days in (from left) Supraside, Acephate, Nitenpyram, and a 1000-fold solution of Hyponex, and Figure 28(2) shows the growth of the white truffle Tuber fungus after 7 days in (from left) PMP, Ethiprole Flowable Buprofezin, and a 1000-fold solution of Hyponex. All insecticides diluted 1000 times showed growth and reproduction speeds equivalent to those of the white truffle Tuber fungus grown on Hyponex medium. This suggests that the white truffle Tuber fungus grew and reproduced using the insecticide components as "food," and that it broke down the insecticide components, thereby "deactivating" their insecticidal ability. Furthermore, the fact that the white truffle Tuber fungus was not adversely affected by the insecticide components indicates that the insecticide and the white truffle Tuber fungus suspension can be mixed and sprayed. By adopting such an embodiment, the insecticide exerts its insecticidal effect while being decomposed by the white truffle Tuber fungus, so that the residual amount of the insecticide can be significantly reduced.

[0072] Test 24 <Insecticidal effect test using various insecticides decomposed and detoxified by white truffle Tuber fungus> Under the same conditions as in Test 23, the white truffle Tuber fungus was added to 1000-fold diluted solutions of various insecticides and cultured to examine the decomposition and detoxification of the various insecticide components. [Test Method] Three days after the start of the incubation, the insecticide solution in which the white truffle Tuber fungus had been cultivated was sprayed on the caterpillars, and the survival status of the caterpillars was visually observed after 24 hours to determine the insecticide decomposition and detoxification effect of the white truffle Tuber fungus. Figure 29 (1A) shows an image of a caterpillar sprayed with a solution of Supracide, which is a culture of the white truffle Tuber fungus. Figure 29 (1B) shows the caterpillars 24 hours after application, and they were still alive. Figure 29 (2A) shows an image of a caterpillar sprayed with an acephate solution containing the white truffle Tuber fungus. Figure 29 (2B) shows the caterpillars 24 hours after application, and they were still alive. Figure 29 (3A) shows an image of a caterpillar sprayed with a solution of nitenpyram cultured with the white truffle Tuber fungus. Figure 29 (3B) shows the caterpillars 24 hours after application, and they were still alive. Figure 29 (4A) shows an image of a caterpillar sprayed with a PMP solution containing cultured white truffle Tuber fungus. Figure 29 (4B) shows the caterpillars 24 hours after application, and they were still alive. Figure 29 (5A) shows an image of a caterpillar sprayed with an ethiprole solution containing cultured white truffle Tuber fungus. Figure 29 (5B) shows the caterpillars 24 hours after application, still alive. Figure 29 (6A) shows an image of a caterpillar sprayed with a flowable solution containing cultured white truffle Tuber fungus. Figure 29 (6B) shows the caterpillars 24 hours after application, and they were still alive. These tests demonstrated that the white truffle Tuber fungus decomposes and detoxifies a variety of insecticides in just three days. This indicates that the mixture of insecticide and white truffle Tuber fungus can decompose residual insecticide quickly after the insecticide takes effect.

[0073] Test 25 <Test on the decomposition and purification of nitrate nitrogen contained in sewage purification residue by the white truffle Tuber fungus> In a decarbonized society, human and livestock waste should be returned to agricultural fields. Food derived from animals and plants is produced using solar energy and the carbon cycle of crops, and it is desirable to return it to the soil rather than incinerating it, so that it can be recycled for food reproduction. Currently, human and livestock waste is decomposed in sewage treatment plants by microorganisms such as Bacillus, Aspidisca, Epistylis, Amoeba, Corella and others, and purified to a certain standard. However, sewage contains refractory organic matter, including lignin, which cannot be decomposed by these bacteria and microorganisms, and this remains as residual sludge. To treat this residue, it is returned to agricultural fields or incinerated, but not only is this very costly, but it also has the problem that crops do not grow well when used as agricultural material, so its use in fields has not progressed. One of the reasons for this is that it is supplied as agricultural material without being denitrified because denitrification is very costly, resulting in an excess of nitrate nitrogen that has a major impact on crop roots. The inventors carried out a habitat and reproduction test of white truffle Tuber fungus by making use of the property that the Tuber fungus can reproduce even in a high concentration ion solution. Furthermore, the inventors mixed the residue from the sewage plant after feeding on the nitrate nitrogen of the white truffle Tuber fungus as "food" into the soil and performed a cultivation test of strawberries and Chinese cabbage. The test conditions are shown in Figures 16-1 to 16-6. Figure 30(1) Sewage treatment plant residue image Figure 30 (2) Image of white truffle Tuber fungus growing on the sewage residue in Photo 1 Figure 30 (3) Poor growth of bok choy cultivated with sewage residue Figure 30 (4) Bok choy grown in 10% akadama soil mixed with the nitrate nitrogen purification residue from the white truffle Tuber fungus shown in Photo 2. Good growth. Figure 30 (5) Chinese cabbage grown in Akadama soil mixed with 10% of the purification residue shown in Photo 2 Figure 30(6) Strawberries grown in Akadama soil mixed with 10% of the purification residue shown in Photo 2 The white truffle fungus Tuber has a strong antibacterial effect against soil-borne pathogens. This antibacterial property is due to the fact that the white truffle Tuber fungus is a mushroom fungus that forms fruit bodies in the soil up to 40 cm deep. In order to form fruit bodies that leave offspring in the soil, it must kill or suppress dormancy of the various microorganisms and pathogenic bacteria already living in the soil. This test was conducted in the hope that mixing residues of the white truffle Tuber fungus into the culture soil would suppress the growth and reproduction of soil-borne pathogens. As expected, no diseases such as soft rot, anthracnose, or fusarium were observed in the crops in the test cultivation. Furthermore, the fungus grew smoothly without any growth problems caused by the high concentration of nitrate nitrogen in sewage residues, which is a problem. This indicates that one of the objects of the present invention can be achieved by using sewage residues, livestock waste, and soil containing residual nitrate nitrogen as excellent agricultural materials by feeding on the nitrate nitrogen contained in the soil and allowing the mycelium to grow. This invention was made possible by using the white truffle Tuber fungus, which is an ascomycete wood-decaying fungus that has the properties of a mycorrhizal fungus that lives symbiotically with plants and crops, rather than using bacteria as in the past.

[0074] Test 26: <Strawberry cultivation in elevated beds and Chinese cabbage in the field using sewage residue purified by white truffle Tuber fungus> Since the results of the experiment25 demonstrated that sewage residue had a positive effect on plant growth and disease control, sewage residue purified with the white truffle Tuber fungus was used in large-area farming to cultivate strawberries in elevated greenhouses and Chinese cabbage in open fields, resulting in excellent growth. In both cases, the disease bacteria was put into hibernation by spraying a 30 to 100 times diluted solution of the white truffle Tuber fungus suspension on the leaves or drenching the soil, making it possible to harvest strawberries and Chinese cabbage that were almost "pesticide-free" without the use of fungicides, and the residual components of the insecticide were decomposed and detoxified by spraying a suspension of the white truffle Tuber fungus. Cultivation method For both strawberries and Chinese cabbage, 200 kg of sewage residue, which is a habitat for the white truffle Tuber fungus, per 10 ares was mulched onto the soil surface. Other cultivation practices were followed. For strawberries, a 30 to 100 times diluted suspension of the white truffle Tuber fungus was sprayed at 10 to 15 day intervals from the time of seedling raising until the end of harvest. The insecticide was sprayed several times, and 24 hours after each spray, a 30-fold diluted solution of the white truffle Tuber fungus suspension was always sprayed over the entire greenhouse to decompose and detoxify any residual pesticide. Chinese cabbage was directly sown in the field, and conventional agricultural chemicals were used, but after the application of the chemicals, a 30-fold diluted suspension of the white truffle Tuber fungus was always sprayed over the entire greenhouse 24 hours later to decompose and detoxify any residual chemicals. Figure 31(1) Image of strawberry cultivation in a greenhouse Figure 31(2) Image of Chinese cabbage grown in an open field

[0075] Test 27 <Test on the effect of foliar spraying of white truffle Tuber fungus culture suspension on plant and crop growth (Test on plant hormone production)> The white truffle fungus Tuber produces the plant hormone indole-3-acetic acid. The purpose of this test is to confirm that the white truffle Tuber fungus produces glucose → indole-pyruvic acid → indole-3-pyruvic acid → indole-3-acetic acid (auxin) using the sugar added to the culture medium during liquid culture by spraying a 30 to 100-fold diluted solution of the culture residue of the white truffle Tuber fungus on the leaves. [Nadeshiko] When a 30 to 100 times diluted solution of the residue of the liquid culture of the white truffle Tuber fungus was sprayed on the leaves of "Nadeshiko" and "Rice", the height of the plants increased by about 20%, and the size of the flowers of "Nadeshiko" also increased by one size. Figure 32(1) Side view of potted dianthus. In the image, the pot on the left has been sprayed with white truffle Tuber fungus culture residue liquid (twice, every 10 days, starting just before flowering), while the pot on the right has not been treated. Figure 32 (2) Image from directly above. The flowers of the dianthus in the pot sprayed with the residue of the white truffle Tuber fungus culture have grown a size larger than those in the untreated pot. [Rice] Akadama soil, medium grain, single use Fertilizer Long slow release fertilizer for rice 6g Base fertilizer Grown in a No. 6 pot using the bottom water absorption method Spraying of 50 times diluted solution of White Truffle Tuber fungal culture suspension (heat inactivated) (performed every 15 days from planting until heading in mid-August) Figure 32 (3) Rice test images (early August, panicle formation stage images), left 3 rows: untreated area, right 3 rows: area sprayed with white truffle Tuber fungus culture residue liquid [result] As is clear from the photo in the figure, the leaves in the untreated area have turned yellow due to lack of fertilizer and damage from high temperatures, but in the area sprayed with the residue of the white truffle Tuber culture, the plant height has grown by about 20% compared to the untreated area, and the leaves are green, showing healthy growth that does not appear to be due to lack of fertilizer. The results of this experiment suggest that spraying the white truffle Tuber fungus suspension on the leaves allows for stable harvests with reduced fertilizer. The fact that the growth of flowers and crops is promoted without fertilization suggests that the white truffle Tuber fungus produces the plant hormone indole-3-acetic acid. This means that spraying the white truffle Tuber fungus suspension in reduced fertilizer cultivation is expected to increase yields by about 20%.

[0076] Test 28 <Growth test of rice sprayed with a suspension of cultured white truffle Tuber fungus (Plant hormone production test 2)> [Test Method] Variety: Tsuyahime · Plant the rice seedlings in No. 6 pots and administer 5g of long rice fertilizer to each pot (this amount of fertilizer will cause the fertilizer to run out after one month). - For rice plants that had run out of fertilizer, a 50-fold diluted suspension of the white truffle Tuber fungus culture was sprayed on the leaves at 15-day intervals for two months (treated area), and six pots of each were prepared as control areas. If the white truffle Tuber fungus culture produces the plant hormone indole-3-acetic acid in the culture suspension, some kind of change should occur when sprayed onto the leaves. Figure 33 (1) Images taken after foliar spraying. The left three rows are untreated areas, and the right three rows are treated areas. As the photograph shows, there was a large difference in growth and plant height between the treated and untreated areas, with the plant height in the treated area being approximately 55 cm and that in the untreated area being 45 cm. There was also a big difference in leaf color; the leaves in the untreated area were yellow-green due to "running out of fertilizer," while the leaves in the treated area were green with no sign of "running out of fertilizer." This phenomenon suggests that the white truffle Tuber culture suspension had an anti-aging effect on the crops. Figure 33 (2) is an image of the untreated area three weeks after the foliar spray. The untreated area (right side) of “Tsuyahime” shows no ears of rice and is growing poorly due to a lack of fertilizer. With this growth rate, almost no harvest can be expected. On the other hand, the rice in the treatment area (left) grew in the same way as in paddy field cultivation, and there was no sign of fertilizer depletion. With this growth, a harvest of about 500 kg per 10 ares can be expected. This experiment suggests that it is possible to "reduced fertilizer cultivation" of white truffle crops by foliar spraying with a suspension of Tuber fungus. It also suggests that it is possible to prevent aging and poor growth caused by lack of fertilizer. The white truffle Tuber fungus of the present invention can be easily cultivated without requiring any special facilities. Therefore, in developing countries, by cultivating the white truffle Tuber fungus of the present invention, producing a culture solution and spraying it on the leaves of crops, a stable harvest can be expected without the use of expensive chemical fertilizers or pesticides.

[0077] Test 29 <Detoxification and decomposition test of grass endophytes by spraying a suspension of white truffle Tuber fungus> Endophytes (endophytic fungi) are fungi or bacteria that live symbiotically within a plant, and the term is a combination of endo (within) and phyte (plant). In general, it refers to fungi of the Ergot family that parasitize grasses. In relation to livestock poisoning, endophytes have become a problem, with tall fescue parasitizing coenophialum (previously called Acremonium) and perennial ryegrass parasitizing N. lolii, and many livestock becoming poisoned when they eat pasture grass in the United States, Australia, and New Zealand. In this way, many microorganisms and mushrooms, such as endophytes, contain components that exhibit toxicity as biologically active substances. However, it is known that all toxic organic compounds produced by organisms in nature are decomposed by wood-rotting fungi and returned to the soil. The white truffle Tuber fungus, a white wood-rotting ascomycete fungus of the present invention, decomposes various pesticide components of organic compounds remaining in crops, and is therefore expected to decompose the toxic components produced by endophytes. Figure 34 (1) shows the actual situation of irrigation and pesticide spraying in a pasture production field in the United States. A suspension of white truffle Tuber fungus was mixed into the irrigation or pesticide solution (insecticide) and sprayed. Figure 34 (2) A photo of the harvesting scene of pasture grass that has been purified by decomposing and detoxifying the "mycotoxin" produced by the endophyte Neotyphodium fungus through spraying of a suspension of the white truffle Tuber fungus (a pasture grass cultivation field in the suburbs of Seattle, USA, where the present invention was tested).

[0078] Test 30 <Crops and plant tissues spray penetration time test of 30 times diluted white truffle Tuber fungus culture suspension> The purpose of this invention is to decompose, detoxify and purify residual pesticides in processed food ingredients during growth and after harvest by spraying or immersing them in a suspension of white truffle Tuber fungus culture. For this purpose, it is absolutely essential that the diluted suspension of white truffle Tuber fungus is rapidly absorbed into the plant tissue. A test was conducted on the time required for absorption. [Test Method] Under conditions of 15°C and 90% humidity, a 30-fold diluted suspension of the white truffle Tuber fungus was sprayed onto Chinese cabbage leaves as test material, and the penetration status was observed over time. Figure 35 (1) Image of white truffle Tuber fungus suspension sprayed 30 times diluted on Chinese cabbage leaves Figure 35(2) State after 5 minutes: Starting to penetrate the leaf cells. Figure 35(3) Condition after 10 minutes: The diluted solution has penetrated the surface of the leaves until it is almost gone.

[0079] Test 31 <White truffle Tuber suspension 30-fold diluted solution skin penetration test> When spraying pesticides on crops, the pesticides are inhaled and adhere to the body during work, which can lead to acute poisoning in some cases. The pesticide components that adhere to the skin penetrate the skin and are absorbed into the body, becoming residual pesticides in the body, which can cause a weakened immune system. We have confirmed that the white truffle Tuber fungus suspension of the present invention is effective not only for crops, but also for decomposing, detoxifying, and purifying residual pesticides that penetrate the skin of agricultural and plant cultivation workers and remain in the body. [Test Method] A 30-fold diluted suspension of the white truffle Tuber fungus was sprayed onto the surface of a human arm, and the penetration of the solution into the skin was observed over time. The temperature was 20°C and the humidity was 90%. Figure 36 (1) The condition of wet skin immediately after spraying a 30-fold diluted suspension of white truffle Tuber on the left arm Figure 36 (2) Skin image 5 minutes after spraying. Skin penetration has begun. Figure 36 (3) Image of skin 10 minutes after spraying. The product has almost completely penetrated the skin, and no liquid or moisture remains on the skin. The results of this test show that after spraying pesticides, workers can decompose and purify residual pesticides in skin cells by washing the areas with pesticide on their faces, hands, etc., and then spraying them with a 30-fold diluted suspension of white truffle Tuber.

Claims

1. A method for decomposing and purifying residual pesticides in materials that contain or have residual pesticides attached thereto, using the white truffle Tuber fungus.

2. 2. The method for decomposing and purifying according to claim 1, wherein the material containing or having residual pesticides attached thereto is a plant, sewage sludge or soil.

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