Bispecific binding construct

Abstract

Novel configurations of bispecific binding constructs are described, as well as methods for their production. Further, uses in therapeutic indications are described.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to U.S. Provisional Patent Application No. 62 / 858,509, filed June 7, 2019, and U.S. Provisional Patent Application No. 62 / 858,630, filed June 7, 2019. Each of the above-identified applications is incorporated herein by reference for all purposes.

[0002] Sequence Listing Reference This application contains a Sequence Listing that has been submitted electronically in ASCII format, which is incorporated herein by reference in its entirety. The ASCII copy, created on June 4, 2020, is named A-2355-WO-PCT_SL.txt and is 174,109 bytes in size.

[0003] The present invention belongs to the field of protein engineering. [Background technology]

[0004] Bispecific binding constructs have recently emerged as promising therapeutic approaches. For example, bispecific constructs targeting both CD3 and CD19 in a bispecific T cell inducer (BiTE®) configuration have shown impressive efficacy at low doses. Bargou et al. (2008), Science 321:974-978. This BiTE® configuration consists of two scFv's, one targeting CD3 and one targeting the tumor antigen CD19, linked by a flexible linker. This unique design allows the bispecific construct to bring activated T cells into close proximity with target cells, resulting in cytolytic killing of the target cells. See, for example, WO 99 / 54440 A1 (U.S. Patent No. 7,112,324 B1) and WO 2005 / 040220 (U.S. Patent Application Publication No. 2013 / 0224205 A1). Later, bispecific constructs have been developed that bind to context-independent epitopes at the N-terminus of the CD3 epsilon chain (see WO 2008 / 119567; US 2016 / 0152707 A1).

[0005] In the biopharmaceutical industry, molecules are typically produced in a large scale to meet commercial demands for patient supply, and multiple attributes can be evaluated to mitigate the risk that the molecule is not suitable for large scale production and purification. Efficient expression of these complex recombinant polypeptides can be an ongoing challenge. Furthermore, once expressed, the polypeptides are often not as stable as desired for pharmaceutical compositions. Thus, there is a need for technologies for bispecific therapeutics that have not only therapeutic efficacy, but also favorable pharmacokinetic properties, and configurations that provide efficient productivity and improved stability. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] International Publication No. 99 / 54440A1 (U.S. Patent No. 7,112,324B1) [Patent Document 2] International Publication No. 2005 / 040220 (US Patent Application Publication No. 2013 / 0224205A1) [Patent Document 3] International Publication No. 2008 / 119567; U.S. Patent Application Publication No. 2016 / 0152707A1 [Non-patent literature]

[0007] [Non-Patent Document 1] Bargou et al. (2008), Science 321:974-978 Summary of the Invention [Means for solving the problem]

[0008] Several novel configurations of bispecific antibodies are described herein. In one embodiment, the invention provides a bispecific binding construct comprising a polypeptide chain comprising an amino acid sequence having the formula VH1-L1-VH2-L2-VL1-L3-VL2, where VH1 and VH2 are immunoglobulin heavy chain variable regions, VL1 and VL2 are immunoglobulin light chain variable regions, L1, L2 and L3 are linkers, L1 is at least 10 amino acids, L2 is at least 15 amino acids, and L3 is at least 10 amino acids, and the bispecific binding construct is capable of binding to immune effector cells and target cells.

[0009] In another embodiment, the invention provides a bispecific binding construct comprising a polypeptide chain comprising an amino acid sequence having the formula VH1-L1-VH2-L2-VL1-L3-VL2, wherein VH1 and VH2 are immunoglobulin heavy chain variable regions, VL1 and VL2 are immunoglobulin light chain variable regions, L1, L2 and L3 are linkers, L1 is at least 10 amino acids, L2 is at least 10 amino acids, L3 is at least 10 amino acids, and the total amino acid length of L1, L2 and L3 is at least 35 amino acids, and wherein the bispecific binding construct is capable of binding to immune effector cells and target cells.

[0010] In another embodiment, the invention provides a bispecific binding construct comprising a polypeptide chain comprising an amino acid sequence having the formula VH1-L1-VH2-L2-VL1-L3-VL2-Fc, wherein VH1 and VH2 are immunoglobulin heavy chain variable regions, VL1 and VL2 are immunoglobulin light chain variable regions, Fc comprises an antibody Fc region (e.g., scFc), L1, L2, L3 and L4 are linkers, L1 is at least 10 amino acids, L2 is at least 10 amino acids, L3 is at least 10 amino acids, and the total amino acid length of L1, L2 and L3 is at least 35 amino acids, and wherein the bispecific binding construct is capable of binding to an immune effector cell and a target cell.

[0011] In further embodiments, the present invention provides nucleic acids encoding the bispecific binding constructs described herein, and vectors comprising these nucleic acids. Additionally, the present invention provides host cells comprising the vectors described herein.

[0012] In yet another embodiment, the invention provides a method for producing a bispecific binding construct described herein, comprising: (1) culturing a host cell under conditions to express the bispecific binding construct; and (2) recovering the binding construct from the cell mass or cell culture supernatant, wherein the host cell comprises one or more nucleic acids encoding any of the bispecific binding constructs described herein.

[0013] In other embodiments, the present invention provides a method of treating a patient with cancer comprising administering to the patient a therapeutically effective amount of a bispecific binding construct described herein.

[0014] In other embodiments, the present invention provides a method of treating a patient having an infectious disease comprising administering to the patient a therapeutically effective amount of a bispecific binding construct described herein.

[0015] In other embodiments, the present invention provides a method of treating a patient suffering from an autoimmune, inflammatory, or fibrotic condition comprising administering to the patient a therapeutically effective amount of a bispecific binding construct described herein.

[0016] In another embodiment, the present invention provides a pharmaceutical composition comprising a bispecific binding construct as described herein. [Brief description of the drawings]

[0017] [Figure 1] FIG. 1 is a representative diagram of an exemplary embodiment of an HHLL configuration. [Diagram 2] Representative diagrams of various molecular configurations, including the standard BiTE configuration of HLHL and various embodiments of HHLL configurations with different linker lengths. [Diagram 3] FIG. 16 is a representative diagram of HLHL BiTE configurations and linker lengths compared to HHLL configurations with representative embodiment linker lengths. [Figure 4A]Figure 4A shows a representative molecular model of the HHLL configuration in a neutral orientation. Figure 4B shows a representative molecular model of the HHLL configuration rotated 90° about the Y axis. Figure 4C shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis. Figure 4D shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis and 90° about the X axis. These models illustrate how the linker lengths relate to each other and how the molecule can properly form its structure. [Figure 4B] Figure 4A shows a representative molecular model of the HHLL configuration in a neutral orientation. Figure 4B shows a representative molecular model of the HHLL configuration rotated 90° about the Y axis. Figure 4C shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis. Figure 4D shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis and 90° about the X axis. These models illustrate how the linker lengths relate to each other and how the molecule can properly form its structure. [Figure 4C] Figure 4A shows a representative molecular model of the HHLL configuration in a neutral orientation. Figure 4B shows a representative molecular model of the HHLL configuration rotated 90° about the Y axis. Figure 4C shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis. Figure 4D shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis and 90° about the X axis. These models illustrate how the linker lengths relate to each other and how the molecule can properly form its structure. [Figure 4D]Figure 4A shows a representative molecular model of the HHLL configuration in a neutral orientation. Figure 4B shows a representative molecular model of the HHLL configuration rotated 90° about the Y axis. Figure 4C shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis. Figure 4D shows a representative molecular model of the HHLL configuration rotated 180° about the Y axis and 90° about the X axis. These models illustrate how the linker lengths relate to each other and how the molecule can properly form its structure. [Figure 5A] Representative examples of purification chromatograms and gel results of different wild-type ("WT") (HLHL) constructs. [Figure 5B] Representative examples of purification chromatograms and gel results of different wild-type ("WT") (HLHL) constructs. [Figure 6A] Representative examples of purification chromatograms and gel results of different HHLL constructs. [Figure 6B] Representative examples of purification chromatograms and gel results of different HHLL constructs. [Figure 7] Representative results of the expression of various HHLL constructs compared to WT. [Figure 8] Representative results of the chemical stability of various HHLL constructs compared to WT. [Figure 9A] Representative results of the thermostability of various HHLL constructs compared to WT. [Figure 9B] Representative results of the thermostability of various HHLL constructs compared to WT. [Figure 10] Representative results of accelerated stability studies at 40° C. of various HHLL constructs compared to WT. Based on molecular modeling, the “332” construct was designed to be a negative control. [Figure 11-1] Representative results of clipping studies of various HHLL constructs compared to WT. [Figure 11-2] Representative results of clipping studies of various HHLL constructs compared to WT. [Figure 12]Representative results of binding studies of various HHLL constructs compared to WT. [Figure 13] Representative results of target cell killing and efficacy of various HHLL constructs compared to WT. [Figure 14] Representative results of various HHLL constructs compared to wild type (WT) at 0, 2, 4, and 8 weeks in a -20°C stability assay. [Figure 15] Representative results of various HHLL constructs compared to wild type (WT) in a freeze-thaw assay. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0018] A novel configuration of bispecific binding constructs is described herein. The constructs comprise a single chain polypeptide consisting of two immunoglobulin variable heavy (VH) regions, two immunoglobulin variable light (VL) regions, and optionally an Fc region (e.g., scFC), arranged in the following order: VH-linker-VH-linker-VL-linker-VL ("HHLL"), or VH-linker-VH-linker-VL-linker-VL-linker-scFc. The HHLL configuration of the bispecific binding constructs provides both improved stability and improved in vitro expression, while maintaining the intended function of binding to desired targets in immune effector cells and target cells, as compared to, for example, the HLHL configuration. Thus, the HHLL configuration provides bispecific molecules that can be produced more efficiently and have better stability, which are desired properties for pharmaceutical compositions.

[0019] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not intended to limit the invention as claimed. In this application, the use of the singular includes the plural unless specifically stated otherwise. In this application, the use of "or" means "and / or" unless specifically stated otherwise. Furthermore, the use of the term "comprising" is not limiting, as are other forms such as "comprises" and "comprises." Additionally, the use of terms such as "element" or "component" encompasses both elements and components comprising one unit, as well as elements and components comprising two or more subunits, unless specifically stated otherwise. Additionally, the use of the term "moiety" can include a portion of a moiety or the entire moiety.

[0020] In this specification, unless otherwise defined, scientific and technical terms used in connection with the present invention shall have the meanings commonly understood by those skilled in the art. Furthermore, unless the context requires otherwise, singular terms shall include the plural and plural terms shall include the singular. In general, the nomenclature and techniques used in connection with cell and tissue culture, molecular biology, immunology, microbiology, genetics, and chemical properties and hybridization of proteins and nucleic acids described herein are those well known and commonly used in the art. The methods and techniques of the present invention are generally carried out according to conventional methods well known in the art, as described in various general and more specific references.

[0021] Polynucleotide and polypeptide sequences are shown using standard one-letter or three-letter abbreviations. Unless otherwise noted, polypeptide sequences have their amino terminus on their left and their carboxy terminus on their right, and single-stranded nucleic acid sequences and the top strand of double-stranded nucleic acid sequences have their 5' terminus on their left and their 3' terminus on their right. Particular sites in a polypeptide can be designated by amino acid residue number, such as amino acids 1-50, or by the actual residue at the site, such as asparagine to proline. A particular polypeptide or polynucleotide sequence can also be described by identifying how it differs from a reference sequence.

[0022] definition The term "isolated" with respect to a molecule (where the molecule is, for example, a polypeptide, a polynucleotide, a bispecific binding construct, or an antibody) refers to a molecule that, depending on its origin or source of derivation, (1) is not associated with naturally associated components that accompany it in its native state, (2) is substantially free of other molecules from the same species, (3) is expressed by cells from a different species, or (4) is not naturally occurring. Thus, a molecule that is chemically synthesized or expressed in a cellular system different from the cell in which it naturally occurs will be "isolated" from its naturally associated components. A molecule may also be rendered substantially free of naturally associated components by isolation using purification techniques well known in the art. The purity or homogeneity of a molecule may be assessed by a number of means well known in the art. For example, the purity of a polypeptide sample may be assessed using polyacrylamide gel electrophoresis and staining the gel using techniques well known in the art to visualize the polypeptide. For certain purposes, higher resolution may be provided by using HPLC or other purification means well known in the art.

[0023] The terms "polynucleotide," "oligonucleotide," and "nucleic acid" are used interchangeably throughout and include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), analogs of DNA or RNA generated using nucleotide analogs (e.g., peptide nucleic acids and non-naturally occurring nucleotide analogs), and hybrids thereof. Nucleic acids can be single-stranded or double-stranded. In one embodiment, a nucleic acid molecule of the invention comprises a contiguous open reading frame encoding a binding construct of the invention or a fragment, derivative, mutein, or variant thereof.

[0024] A "vector" refers to a nucleic acid that can be used to introduce another nucleic acid linked to it into a cell. One type of vector, a "plasmid," refers to a linear or circular double-stranded DNA molecule to which additional nucleic acid segments can be linked. Another type of vector is a viral vector (e.g., replication defective retroviruses, adenoviruses, and adeno-associated viruses), which allows additional DNA segments to be introduced into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors containing a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of the host cell upon introduction into the host cell, and are thereby replicated along with the host genome. An "expression vector" refers to a type of vector that can direct the expression of a selected polynucleotide.

[0025] A nucleotide sequence is "operably linked" to a regulatory sequence if the regulatory sequence affects expression of the nucleotide sequence (e.g., the level, timing, or location of expression). A "regulatory sequence" is a nucleic acid that affects expression (e.g., the level, timing, or location of expression) of an operably linked nucleic acid. A regulatory sequence can exert an effect, for example, directly on the nucleic acid being regulated or through the action of one or more other molecules (e.g., regulatory sequences and / or polypeptides that bind to the nucleic acid). Examples of regulatory sequences include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals).

[0026] A "host cell" refers to a cell that can be used to express a nucleic acid, such as a nucleic acid of the invention. A host cell can be a prokaryote, such as E. coli, or a eukaryote, such as a unicellular eukaryote (e.g., yeast or other fungi), a plant cell (e.g., tobacco or tomato plant cell), an animal cell (e.g., a human cell, a monkey cell, a hamster cell, a rat cell, a mouse cell, or an insect cell), or a hybridoma. Typically, a host cell is a cultured cell that can be transformed or transfected with a nucleic acid encoding a polypeptide, and then the host cell can express the nucleic acid. The phrase "recombinant host cell" can be used to refer to a host cell that has been transformed or transfected with a nucleic acid to be expressed. A host cell can also be a cell that contains a nucleic acid but does not express the nucleic acid at a desired level unless a regulatory sequence is introduced into the host cell such that the regulatory sequence is operably linked to the nucleic acid. It will be understood that the term host cell refers not only to the particular subject cell, but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in subsequent generations due, for example, to mutations or environmental influences, such progeny may not be effectively identical to the parent cell, but are still included within the scope of the term as used herein.

[0027] A "single-chain variable fragment" ("scFv") is a fusion protein in which a VL domain and a VH domain are linked via a linker (e.g., a synthetic sequence of amino acid residues) to form a contiguous protein chain, the linker being of sufficient length to allow the protein chain to refold on itself and form a monovalent antigen-binding site (see, e.g., Bird et al., Science 242:423-26 (1988) and Huston et al., 1988. Proc. Natl. Acad. Sci. USA 85:5879-83 (1988)). When associated with additional moieties (e.g., an Fc domain), the scFv is arranged, for example, VH-linker-VL, or VL-linker-VH.

[0028] The term "CDR" refers to the complementarity determining regions (also called "minimal recognition units" or "hypervariable regions") within an antibody variable sequence, and the bispecific binding construct of the present invention comprises heavy and / or light chain CDRs. The CDRs enable the binding construct to specifically bind to a particular antigen of interest. There are three heavy chain variable region CDRs (CDRH1, CDRH2 and CDRH3) and three light chain variable region CDRs (CDRL1, CDRL2 and CDRL3). The CDRs of each of the two chains are typically aligned by framework regions to form a structure that specifically binds to a particular epitope or domain on the target protein. Both naturally occurring light and heavy chain variable regions, from N-terminus to C-terminus, typically follow the following order of these elements: FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4. A numbering system has been devised to assign numbers to the amino acids occupying each position in these domains. This numbering system is defined in Kabat Sequences of Proteins of Immunological Interest (1987 and 1991, NIH, Bethesda, MD) or Chothia & Lesk, 1987, J. Mol. Biol. 196:901-917; Chothia et al., 1989, Nature 342:878-883. The complementarity determining regions (CDRs) and framework regions (FRs) of a given antibody can be identified using this system. Other numbering systems for the amino acids of immunoglobulin chains include IMGT® (international ImMunoGeneTics information system; Lefranc et al., Dev. Comp. Immunol. 29:185-203; 2005) and AHo (Honegger and Pluckthun, J. Mol. Biol. 309(3):657-670; 2001). One or more CDRs may be incorporated into a molecule, either covalently or non-covalently, to form a binding construct.

[0029] The "binding domain" of the binding construct according to the invention may, for example, comprise the CDRs of the above-mentioned group. Preferably, those CDRs are comprised within the framework of the antibody light chain variable region (VL) and the antibody heavy chain variable region (VH) constituted by the bispecific binding construct of the invention. Alternatively, as the term is used herein, the "L" and "H" variable regions (e.g. "HHLL").

[0030] The term "human antibody" includes antibodies having antibody regions, such as variable and constant regions or domains, that substantially correspond to human germline immunoglobulin sequences known in the art, including, for example, those described by Kabat et al. (1991) supra. Human antibodies as referred to herein may contain amino acid residues not encoded by human germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo), for example in the CDRs, particularly CDR3. Human antibodies may have at least one, two, three, four, five or more positions replaced with amino acid residues not encoded by human germline immunoglobulin sequences. As used herein, the definition of human antibody also contemplates fully human antibodies that contain only human sequences of non-artificial and / or genetically engineered antibodies, such as may be derived by using techniques or systems known in the art, such as, for example, phage display technology or transgenic mouse technology, including, but not limited to, Xenomouse®. In the context of the present invention, variable regions derived from human antibodies can be used in the construction of the bispecific binding constructs envisaged.

[0031] A humanized antibody has a sequence that differs from that of an antibody derived from a non-human species by one or more amino acid substitutions, deletions, and / or additions, such that the humanized antibody is less likely to induce an immune response and / or induces a less severe immune response when administered to a human subject compared to the non-human species antibody. In one embodiment, certain amino acids within the framework and heavy and / or light chain constant domains of a non-human species antibody are mutated to generate a humanized antibody. In another embodiment, a constant domain from a human antibody is fused to a variable domain of a non-human species. In another embodiment, one or more amino acid residues within one or more CDR sequences of a non-human antibody are altered to likely reduce the immunogenicity of the non-human antibody when administered to a human subject, provided that none of the altered amino acid residues are critical to the antibody specific binding of the antibody or binding construct to its antigen, or that the changes made to the amino acid sequence are conservative changes, such that the binding of the humanized antibody to the antigen is not significantly worse than the binding of the non-human antibody to the antigen. Examples of methods for making humanized antibodies can be found in U.S. Patent Nos. 6,054,297, 5,886,152, and 5,877,293. In the context of the present invention, variable regions from humanized antibodies can be used in the construction of the bispecific binding constructs envisioned.

[0032] The term "chimeric antibody" refers to an antibody that contains one or more regions from one antibody and one or more regions from one or more other antibodies. In one embodiment, one or more of the CDRs are from a human antibody. In another embodiment, all of the CDRs are from a human antibody. In another embodiment, CDRs from two or more human antibodies are mixed and combined into a chimeric antibody. For example, a chimeric antibody may contain CDR1 from a light chain of a first human antibody, CDR2 and CDR3 from a light chain of a second human antibody, and CDRs from a heavy chain from a third antibody. Furthermore, the framework regions may be from the same antibody, one or more different antibodies, such as a human antibody, or one of a humanized antibody. In one example of a chimeric antibody, a portion of the heavy and / or light chain is identical to, homologous to, or derived from an antibody from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain is identical to, homologous to, or derived from an antibody from another species or belonging to another antibody class or subclass. Also included are fragments of such antibodies which exhibit the desired biological activity. In the context of the present invention, variable regions from chimeric antibodies can be used in the construction of the bispecific binding constructs envisaged.

[0033] The present invention provides a bispecific binding construct consisting of an HHLL construct. Most commonly, a bispecific binding construct as described herein comprises several polypeptide chains with different amino acid sequences that, when linked together, are capable of binding to two different antigens. Optionally, the HHLL molecule further comprises a half-life extending moiety. In some embodiments, the half-life extending moiety is an Fc polypeptide chain. In some embodiments, the half-life extending moiety is a single-chain Fc. In yet other embodiments, the half-life extending moiety is a hetero-Fc. In yet other embodiments, the half-life extending moiety is human albumin.

[0034] Linker Between the immunoglobulin variable regions there is a peptide linker, which may be the same linker or different linkers of different lengths. The linker may play an important role in the construction of the bispecific binding construct, and the invention described herein provides not only the appropriate linker sequence, but also the appropriate linker length for each position in the bispecific binding construct of the invention. If the linker is too short, it may not provide enough flexibility for the appropriate variable regions on a single polypeptide chain to interact and form an antigen binding site. If the linker is of the appropriate length, it allows the variable region to interact with another variable region on the same polypeptide chain to form an antigen binding site. In certain embodiments, the HHLL configuration includes disulfide bonds both intradomain (intradomain H1, L1) and interdomain (between H1 and L1). In certain embodiments, to achieve the proper expression and conformation of the bispecific binding construct of the invention, certain linkers are used between the various immunoglobulin regions (see, for example, FIG. 1 herein). Exemplary linkers are shown in Table 1 herein. In certain embodiments, increasing the linker length may result in the undesirable property of increased protein clipping. It is therefore desirable to strike an appropriate balance between linker length so as to allow for proper polypeptide structure and activity, while not resulting in increased clipping.

[0035] "Linker" as meant herein is a peptide that links two polypeptides. In certain embodiments, a linker can link two immunoglobulin variable regions in the context of a bispecific binding construct. A linker can be 2-30 amino acids in length. In some embodiments, a linker can be 2-25, 2-20, or 3-18 amino acids in length. In some embodiments, a linker can be a peptide that is 14, 13, 12, 11, 10, 9, 8, 7, 6, or 5 amino acids or less in length. In other embodiments, a linker can be 5-25, 5-15, 4-11, 10-20, or 20-30 amino acids in length. In other embodiments, the linker may be about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids in length. Exemplary linkers include, for example, the amino acid sequences GGGGS (SEQ ID NO:1), GGGGSGGGGS (SEQ ID NO:2), GGGGSGGGGSGGGGGS (SEQ ID NO:3), GGGGSGGGGSGGGGSGGGGS (SEQ ID NO:4), GGGGSGGGGSGGGGSGGGGSGGGGGS (SEQ ID NO:5), GGGGQ (SEQ ID NO:6), GGGGQGGGGQ (SEQ ID NO:7), GGGGQGGGGQGGGGQ (SEQ ID NO:8), GGGGQGGGGQGGGGQGGGGQ (SEQ ID NO:9), GGGGQGGGGQGGGGQGGGGQGGGGQ (SEQ ID NO:10), GGGGSAAA (SEQ ID NO:11), TVAAP (SEQ ID NO:12), ASTKGP (SEQ ID NO:13), and AAA (SEQ ID NO:14), in particular those that include repeats of the above-mentioned amino acid sequences or subunits of amino acid sequences (e.g., repeats of GGGGS (SEQ ID NO:1) or GGGGQ (SEQ ID NO:6)).

[0036] In certain embodiments in the context of the HHLL molecules of the invention, the linker sequence of linker 1 is at least 10 amino acids. In other embodiments, linker 1 is at least 15 amino acids. In other embodiments, linker 1 is at least 20 amino acids. In other embodiments, linker 1 is at least 25 amino acids. In other embodiments, linker 1 is at least 30 amino acids. In other embodiments, linker 1 is 10-30 amino acids. In other embodiments, linker 1 is 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids. In yet other embodiments, linker 1 is more than 30 amino acids.

[0037] In certain embodiments in the context of the HHLL molecules of the invention, the linker sequence of linker 2 is at least 15 amino acids. In other embodiments, linker 2 is at least 20 amino acids. In other embodiments, linker 2 is at least 25 amino acids. In other embodiments, linker 2 is at least 30 amino acids. In other embodiments, linker 2 is 15-30 amino acids. In other embodiments, linker 2 is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids. In yet other embodiments, linker 2 is more than 30 amino acids.

[0038] In certain embodiments in the context of the HHLL molecules of the invention, the linker sequence of linker 3 is at least 15 amino acids. In other embodiments, linker 3 is at least 20 amino acids. In other embodiments, linker 3 is at least 25 amino acids. In other embodiments, linker 3 is at least 30 amino acids. In other embodiments, linker 3 is 15-30 amino acids. In other embodiments, linker 3 is 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids. In yet other embodiments, linker 3 is more than 30 amino acids.

[0039] In certain embodiments in the context of the HHLL molecules of the invention, the linker sequence of linker 4 is at least 5 amino acids. In other embodiments, linker 4 is at least 10 amino acids. In other embodiments, linker 4 is at least 15 amino acids. In other embodiments, linker 4 is at least 20 amino acids. In other embodiments, linker 4 is at least 25 amino acids. In other embodiments, linker 4 is at least 30 amino acids. In other embodiments, linker 4 is 5-30 amino acids. In other embodiments, linker 4 is 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids. In yet other embodiments, linker 4 is more than 30 amino acids.

[0040] 4A-4D show molecular models of various orientations of the HHLL construct and indicate what specific linker lengths are required to allow the HHLL construct to adopt the proper conformation and allow both HL binding domains to function. A, B, and C represent the distance between the terminal residue of one domain and the Cα atom of the starting residue of the other domain. Using this information, a skilled practitioner could model a proposed HHLL construct and adjust the linker length required for a particular HL binding domain so that the HHLL construct will express and function as desired.

[0041] In a specific embodiment in the context of the HHLL molecule of the present invention, the linker sequences and positions are set out in Table 1 below, the linker positions correspond to those set out in Figure 1, and linker 4 is optionally used if an Fc region is further attached to the HHLL molecule.

[0042] [Table 1]

[0043] Note that the 3-3-2 linker was intentionally designed with a suboptimal length to serve as a negative control.

[0044] Amino acid sequence of the binding region In the exemplary embodiments described herein, the bispecific binding constructs maintain the desired binding to a variety of desired targets, provided that the appropriate conformation allows for such binding. Immunoglobulin variable regions contain VH and VL domains, which associate to form a variable domain that binds to the desired target.

[0045] The variable domains can be obtained from any immunoglobulin with the desired properties, and methods for achieving this are further described herein. In one embodiment, VH1 and VL1 associate to bind CD3ε, and VH2 and VL2 associate to bind different targets. In another embodiment, VH2 and VL2 bind CD3ε, and VH1 and VL1 bind different targets.

[0046] In another embodiment, the light chain variable domain comprises an amino acid sequence that is at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identical to a sequence of a light chain variable domain described herein.

[0047] In another embodiment, the light chain variable domain comprises an amino acid sequence encoded by a nucleotide sequence at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identical to a polynucleotide sequence described herein. In another embodiment, the light chain variable domain comprises an amino acid sequence encoded by a polynucleotide that hybridizes under moderately stringent conditions to the complement of a polynucleotide encoding a light chain variable domain selected from the sequences described herein. In another embodiment, the light chain variable domain comprises an amino acid sequence encoded by a polynucleotide that hybridizes under stringent conditions to the complement of a polynucleotide encoding a light chain variable domain selected from the group consisting of the sequences described herein.

[0048] In another embodiment, the heavy chain variable domain comprises an amino acid sequence at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identical to a sequence of a heavy chain variable domain selected from the sequences described herein. In another embodiment, the heavy chain variable domain comprises an amino acid sequence encoded by a nucleotide sequence at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identical to a nucleotide sequence encoding a heavy chain variable domain selected from the sequences described herein. In another embodiment, the heavy chain variable domain comprises an amino acid sequence encoded by a polynucleotide that hybridizes under moderately stringent conditions to the complement of a polynucleotide encoding a heavy chain variable domain selected from the sequences described herein. In another embodiment, the heavy chain variable domain comprises an amino acid sequence encoded by a polynucleotide that hybridizes under stringent conditions to the complement of a polynucleotide encoding a heavy chain variable domain selected from the sequences described herein.

[0049] replacement It will be understood that the bispecific binding constructs of the invention may have at least one amino acid substitution, provided that the binding construct retains the same or better desired binding specificity (e.g., binding to CD3). Thus, modifications to the structure of the binding construct are encompassed within the scope of the invention. In one embodiment, the binding construct comprises sequences that differ independently from the CDR sequences as described herein by the addition, substitution and / or deletion of 5, 4, 3, 2, 1 or 0 single amino acids. As used herein, a CDR sequence that differs from the CDR sequences described herein by, for example, the addition, substitution and / or deletion of 4 or less amino acids in total means a sequence that has 4, 3, 2, 1 or 0 single amino acid additions, substitutions and / or deletions compared to the sequences described herein. These may include amino acid substitutions that may be conservative or non-conservative, provided that they do not impair the desired binding ability of the binding construct. Conservative amino acid substitutions may include non-naturally occurring amino acid residues, which are typically incorporated by chemical peptide synthesis rather than by synthesis in a biological system. These include peptidomimetics and other forms in which amino acid moieties are reversed or inverted. Conservative amino acid substitutions can also include substitutions of natural amino acid residues for standard residues that have little or no effect on the polarity or charge of the amino acid residue at that position.

[0050] Non-conservative substitutions may also include the replacement of a member of one class of amino acid or amino acid mimetic with a member from another class that differs in physical properties (e.g., size, polarity, hydrophobicity, charge). In certain embodiments, such substituted residues may be introduced into regions of human antibodies that are homologous with non-human antibodies, or into the non-homologous regions of the molecule, which can be used to generate binding constructs of the invention.

[0051] Furthermore, one skilled in the art can generate test variants containing single amino acid substitutions at each desired amino acid residue. The variants can then be screened using activity assays known to those skilled in the art. Such variants can be used to gather information about suitable variants. For example, if one finds a modification to a particular amino acid residue that is destroyed, undergoes undesirable reduction, or results in inappropriate activity, variants with such modifications can be avoided. In other words, one skilled in the art can easily determine, based on the information gathered from such routine experiments, which amino acids need to be avoided for further substitutions, either alone or in combination with other mutations.

[0052] Those skilled in the art will be able to use well-known techniques to determine suitable variants of binding constructs as described herein. In certain embodiments, those skilled in the art can identify suitable regions of the molecule that may be altered without impairing activity by targeting regions that are not believed to be important for activity. In certain embodiments, residues and portions of the molecule that are conserved among similar polypeptides, as described above, may also be identified. In certain embodiments, even regions that may be important for biological activity or structure may be subject to conservative amino acid substitutions without impairing biological activity or adversely affecting the polypeptide structure.

[0053] Additionally, one skilled in the art can consider structure-function studies that identify residues in similar polypeptides that are important for activity or structure. By considering such comparisons, one can predict the importance of amino acid residues in a protein that correspond to amino acid residues that are important for the activity or structure of the similar protein. One skilled in the art can select chemically similar amino acid substitutions for such predicted important amino acid residues.

[0054] In some embodiments, one of skill in the art can identify residues that may be altered to provide desired improved properties, e.g., amino acid substitutions (conservative or non-conservative) can provide enhanced binding affinity for a desired target.

[0055] A person skilled in the art can also analyze the three-dimensional structure and amino acid sequence with respect to the structure of similar polypeptides. Taking such information into account, a person skilled in the art can predict the alignment of amino acid residues of an antibody with respect to its three-dimensional structure. In certain embodiments, a person skilled in the art can select amino acid residues predicted to be present on the surface of a protein so that such residues are not radically changed, since such residues may be involved in important interactions with other molecules. Many scientific publications have been devoted to the prediction of secondary structure. See Moult J., Curr. Op. in Biotech., 7(4):422-427(1996); Chou et al., Biochemistry, 13(2):222-245(1974); Chou et al., Biochemistry, 113(2):211-222(1974); Chou et al., Adv. Enzymol. Relat. Areas Mol. Biol., 47:45-148(1978); Chou et al., Ann. Rev. Biochem., 47:251-276 and Chou et al., Biophys. J., 26:367-384(1979). In addition, computer programs are now available to assist in the prediction of secondary structure. One method of predicting secondary structure is based on homology modeling. For example, two polypeptides or proteins with greater than 30% sequence identity or greater than 40% similarity often have similar structural topologies. The development of the protein structural database (PDB) has improved the accuracy of predicting secondary structure, including the potential number of folds within a polypeptide or protein structure. See Holm et al., Nucl. Acid. Res., 27(1):244-247 (1999).Additional methods for predicting secondary structure include "threading" (Jones, D., Curr. Opin. Struct. Biol., 7(3):377-87 (1997); Sippl et al., Structure, 4(1):15-19 (1996)), "profile analysis" (Bowie et al., Science, 253:164-170 (1991); Gribskov et al., Meth. Enzym., 183:146-159 (1990); Gribskov et al., Proc. Nat. Acad. Sci., 84(13):4355-4358 (1987)), and "evolutionary linkage" (see Holm (1999), supra, and Brenner (1997), supra).

[0056] In certain embodiments, variants of the binding construct include glycosylation variants in which the number and / or type of glycosylation sites are altered compared to the amino acid sequence of the parent polypeptide. In certain embodiments, variants contain more or fewer N-linked glycosylation sites than the native protein. Alternatively, substitutions that delete this sequence remove existing N-linked carbohydrate chains. Also, rearrangements of N-linked carbohydrate chains are provided in which one or more N-linked glycosylation sites (typically those that occur naturally) are deleted to generate one or more new N-linked sites. Further variants include cysteine ​​variants in which one or more cysteine ​​residues are deleted or replaced by another amino acid (e.g., serine) compared to the parent amino acid sequence. Cysteine ​​variants may be useful when the antibody or bispecific construct needs to be refolded into a biologically active conformation, such as after isolation of insoluble inclusion bodies. Cysteine ​​variants generally have fewer cysteine ​​residues than the native protein, and typically have an even number of cysteine ​​residues to minimize interactions resulting from unpaired cysteines.

[0057] Desired amino acid substitutions (whether conservative or non-conservative) can be determined by one of skill in the art at the time such substitutions are desired. In certain embodiments, amino acid substitutions can be used to identify key residues of a binding construct for a target of interest, or to increase or decrease the affinity of a binding construct for a target of interest as described herein.

[0058] According to certain embodiments, the desired amino acid substitutions are those that (1) reduce susceptibility to proteolysis, (2) reduce susceptibility to oxidation, (3) change the binding affinity for forming protein complexes, (4) change the binding affinity, and / or (4) confer or modify other physiochemical or functional properties to such polypeptides. According to certain embodiments, single or multiple amino acid substitutions (in certain embodiments, conservative amino acid substitutions) may be made within the naturally occurring sequence (in certain embodiments, in the portion of the polypeptide outside the domains that form intermolecular contacts). In certain embodiments, conservative amino acid substitutions typically cannot substantially alter the structural features of the parent sequence (e.g., the replacement amino acid should not tend to disrupt helices present in the parent sequence or other types of secondary structures that characterize the parent sequence). Examples of polypeptide secondary and tertiary structures known to those of skill in the art are described in Proteins, Structures and Molecular Principles (Creighton, Ed., W.H. Freeman and Company, New York (1984)); Introduction to Protein Structure (C. Branden and J. Tooze, eds., Garland Publishing, New York, NY (1991)); and Thornton et al. Nature 354:105 (1991), each of which is incorporated herein by reference.

[0059] Half-life extension and Fc region In certain embodiments, it is desirable to extend the in vivo half-life of the bispecific binding construct of the present invention. This can be achieved by including a half-life extending moiety as part of the bispecific binding construct. Non-limiting examples of half-life extending moieties include Fc polypeptides, albumin, albumin fragments, moieties that bind to albumin or neonatal Fc receptor (FcRn), derivatives of fibronectin modified to bind to albumin or a fragment thereof, peptides, single domain protein fragments, or other polypeptides that can increase serum half-life. In alternative embodiments, the half-life extending moiety can be a non-polypeptide molecule, such as, for example, polyethylene glycol (PEG).

[0060] As used herein, the term "Fc polypeptide" includes native and mutein forms of polypeptides derived from the Fc region of an antibody. Also included are truncated forms of such polypeptides that contain the hinge region that promotes dimerization. In addition to other properties described herein, polypeptides that contain an Fc portion offer the advantage that they can be purified, for example, by affinity chromatography on a Protein A or Protein G column.

[0061] In certain embodiments, the half-life extending moiety is an Fc region of an antibody. The Fc region may be located at the N-terminal end of the HHLL bispecific binding construct or at the C-terminal end of the HHLL bispecific binding construct. There may be, but need not be, a linker between the HHLL bispecific binding construct and the Fc region. As explained above, the Fc polypeptide chain may include all or part of the hinge region, followed by the CH2 and CH3 regions. The Fc polypeptide chain may be of mammalian (e.g., human, mouse, rat, rabbit, dromedary, or New or Old World monkey), avian, or shark origin. In addition, as explained above, the Fc polypeptide chain may include a limited number of mutations. For example, the Fc polypeptide chain may include one or more heterodimerization mutations, one or more mutations that inhibit or enhance binding to FcγR, or one or more mutations that increase binding to FcRn.

[0062] In certain embodiments, the Fc utilized to extend half-life is a single chain Fc ("scFc").

[0063] In some embodiments, the amino acid sequence of the Fc polypeptide may be a mammalian, e.g., human, amino acid sequence. The isotype of the Fc polypeptide may be IgG, such as IgG1, IgG2, IgG3, or IgG4, IgA, IgD, IgE, or IgM. An alignment of the amino acid sequences of the Fc polypeptide chains of human IgG1, IgG2, IgG3, and IgG4 is shown in Table 2 below.

[0064] Usable sequences of the Fc polypeptide chains of human IgG1, IgG2, IgG3, and IgG4 are shown in SEQ ID NOs: 36 to 39. Variants of these sequences containing one or more heterodimerization mutations, one or more Fc mutations that extend half-life, one or more mutations that enhance ADCC, and / or one or more mutations that inhibit Fcγ receptor (FcγR) binding are also contemplated, as are other similar variants that include up to 10 single amino acid deletions, insertions, or substitutions per 100 amino acids of the sequences.

[0065] [Table 2]

[0066] The numbering shown in Table 2 follows the EU numbering system, which is based on the consecutive numbering of the constant regions of IgG1 antibodies. Edelman et al. (1969), Proc. Natl. Acad. Sci. 63:78-85. This numbering therefore does not fully accommodate the extra length of the IgG3 hinge. Nevertheless, this numbering is used herein to denote positions within the Fc region, since this numbering is still commonly used in the art to refer to positions within the Fc region. The hinge regions of the IgG1, IgG2 and IgG4 Fc polypeptides extend from about position 216 to about position 230. It is clear from the alignment that the IgG2 and IgG4 hinge regions are each three amino acids shorter than the IgG1 hinge. The IgG3 hinge is significantly longer, stretching an additional 47 amino acids upstream. The CH2 region extends from approximately positions 231-340, and the CH3 region extends from approximately positions 341-447.

[0067] The naturally occurring amino acids of the Fc polypeptide may vary slightly. Such variations include insertions, deletions, or substitutions of no more than 10 single amino acids per 100 amino acids in the sequence of the naturally occurring Fc polypeptide chain. If substitutions are present, they may be conservative amino acid substitutions as defined above. The Fc polypeptides on the first and second polypeptide chains may differ in amino acid sequence. In some embodiments, they may include "heterodimerization mutations", e.g., charge pairing substitutions that promote heterodimer formation as defined above. Additionally, the Fc polypeptide portion of the PABP may include mutations that block or enhance FcγR binding. Such mutations are described above and in Xu et al. (2000), Cell Immunol. 200(1):16-26, the relevant portions of which are incorporated herein by reference. The Fc polypeptide portion may also include "half-life extending Fc mutations," as defined above, including, for example, those described in U.S. Patent Nos. 7,037,784, 7,670,600, and 7,371,827, U.S. Patent Application Publication No. 2010 / 0234575, and International Application No. PCT / US2012 / 070146, all relevant portions of which are incorporated herein by reference. Additionally, the Fc polypeptide may include "ADCC enhancing mutations," as defined above.

[0068] Another suitable Fc polypeptide, described in PCT Publication WO 93 / 10151 (herein incorporated by reference), is a single chain polypeptide extending from the N-terminal hinge region to the native C-terminus of the Fc region of a human IgG1 antibody. Another useful Fc polypeptide is the Fc mutein described in U.S. Pat. No. 5,457,035 and Baum et al., 1994, EMBO J. 13:3992-4001. The amino acid sequence of this mutein is identical to that of the native Fc sequence presented in WO 93 / 10151, except that amino acid 19 is changed from Leu to Ala, amino acid 20 is changed from Leu to Glu, and amino acid 22 is changed from Gly to Ala. This mutein exhibits reduced affinity for Fc receptors.

[0069] By introducing one or more mutations into the Fc, the effector function of the antibody can be increased or decreased. Embodiments of the invention include IL-2 mutein Fc fusion proteins with modified Fc to increase effector function (U.S. Patent No. 7,317,091 and Strohl, Curr. Opin. Biotech., 20:685-691, 2009; both of which are incorporated by reference herein in their entirety). For certain therapeutic indications, it may be desirable to increase effector function. For other therapeutic indications, it may be desirable to decrease effector function.

[0070] Exemplary IgG1 Fc molecules with enhanced effector function include those having the following substitutions: S239D / I332E S239D / A330S / I332E S239D / A330L / I332E S298A / D333A / K334A P247I / A339D P247I / A339Q D280H / K290S D280H / K290S / S298D D280H / K290S / S298V F243L / R292P / Y300L F243L / R292P / Y300L / P396L F243L / R292P / Y300L / V305I / P396L G236A / S239D / I332E K326A / E333A K326W / E333S K290E / S298G / T299A K290N / S298G / T299A K290E / S298G / T299A / K326E K290N / S298G / T299A / K326E

[0071] Another way to increase the effector function of IgG Fc-containing proteins is by reducing Fc fucosylation. Removal of core fucose from biantennary complex oligosaccharides attached to Fc significantly increases ADCC effector function without altering antigen binding or CDC effector function. Several methods are known to reduce or eliminate fucosylation of Fc-containing molecules, such as antibodies. These methods include recombinant expression in certain mammalian cell lines, including FUT8 knockout cell lines, mutant CHO line Lec13, rat hybridoma cell line YB2 / 0, cell lines containing small interfering RNA specific for the FUT8 gene, and cell lines co-expressing α-1,4-N-acetylglucosaminyltransferase III and Golgi α-mannosidase II. Alternatively, Fc-containing molecules may be expressed in non-mammalian cells, such as plant cells, yeast, or prokaryotic cells, such as E. coli.

[0072] In certain embodiments of the invention, the bispecific binding construct comprises an Fc that has been modified to reduce effector function. Exemplary Fc molecules with reduced effector function include those having the following substitutions: N297A or N297Q (IgG1) L234A / L235A(IgG1) V234A / G237A(IgG2) L235A / G237A / E318A(IgG4) H268Q / V309L / A330S / A331S(IgG2) C220S / C226S / C229S / P238S(IgG1) C226S / C229S / E233P / L234V / L235A(IgG1) L234F / L235E / P331S(IgG1) S267E / L328F(IgG1)

[0073] Human IgG1 has a glycosylation site at N297 (numbering system), and glycosylation is known to contribute to the effector function of IgG1 antibodies. An exemplary IgG1 sequence is shown in SEQ ID NO: 36. To generate an aglycosylated antibody, N297 may be mutated. For example, the mutation can replace N297 with an amino acid similar in physiochemical properties to asparagine, such as glutamine (N297Q), or with alanine (N297A), which mimics asparagine without the polar group.

[0074] In certain embodiments, mutating amino acid N297 of human IgG1 to glycine, i.e., N297G, results in much better purification efficiency and biophysical properties compared to other amino acid substitutions at that residue. See, e.g., U.S. Patent Nos. 9,546,203 and 10,093,711. In certain embodiments, the bispecific binding construct of the invention comprises an Fc of human IgG1 with an N297G substitution.

[0075] The bispecific binding construct of the invention comprising a human IgG1 Fc with a N297G mutation may also comprise further insertions, deletions and substitutions. In certain embodiments, the human IgG1 Fc comprises a N297G substitution and is at least 90% identical, at least 91% identical, at least 92% identical, at least 93% identical, at least 94% identical, at least 95% identical, at least 96% identical, at least 97% identical, at least 98% identical, or at least 99% identical to the amino acid sequence set forth in SEQ ID NO: 36. In particularly preferred embodiments, the C-terminal lysine residue is substituted or deleted.

[0076] In some cases, aglycosylated IgG1 Fc-containing molecules may be less stable than glycosylated IgG1 Fc-containing molecules. Thus, the Fc region may be modified to further increase the stability of the aglycosylated molecules. In some embodiments, one or more amino acids are substituted with cysteine ​​to form disulfide bonds in the dimeric state. In certain embodiments, residues V259, A287, R292, V302, L306, V323, or I332 of the amino acid sequence set forth in SEQ ID NO: 36 may be substituted with cysteine. In other embodiments, certain pairs of residues are substituted to preferentially form disulfide bonds with each other, thereby limiting or preventing scrambling of disulfide bonds. In certain embodiments, the pairs include, but are not limited to, A287C and L306C, V259C and L306C, R292C and V302C, and V323C and I332C.

[0077] As described herein above in the section on linkers, in certain embodiments, the bispecific binding construct of the invention comprises a linker between the Fc and the HHLL bispecific binding construct, in particular linking the Fc to VL2. In certain embodiments, one or more copies of the peptide between the Fc and the HHLL polypeptide comprise GGGGS (SEQ ID NO: 1), GGNGT (SEQ ID NO: 15), or YGNGT (SEQ ID NO: 16). In some embodiments, the polypeptide region between the Fc region and the HHLL polypeptide comprises a single copy of GGGGS (SEQ ID NO: 1), GGNGT (SEQ ID NO: 15), or YGNGT (SEQ ID NO: 16). In certain embodiments, the linker GGNGT (SEQ ID NO: 15) or YGNGT (SEQ ID NO: 16) is glycosylated when expressed in a suitable cell, and such glycosylation can promote stabilization of the protein in solution and / or when administered in vivo. Thus, in certain embodiments, the bispecific binding construct of the invention comprises a glycosylated linker between the Fc region and the HHLL polypeptide.

[0078] Nucleic Acids Encoding Bispecific Binding Constructs In another embodiment, the present invention provides isolated nucleic acid molecules encoding the bispecific binding constructs of the present invention. Additionally, vectors comprising the nucleic acid, cells comprising the nucleic acid, and methods of making the binding constructs of the present invention are provided. Nucleic acids include, for example, polynucleotides encoding all or part of the bispecific binding construct, or fragments, derivatives, muteins, or variants thereof, polypeptides that inhibit expression of the polynucleotide, polynucleotides sufficient for use as hybridization probes, PCR primers, or sequencing primers to identify, analyze, mutate, or amplify polynucleotides encoding antisense nucleic acids, and complementary sequences of the above. Nucleic acids may be of any length suitable for a desired use or function, and may include one or more additional sequences, such as regulatory sequences, and / or part of a larger nucleic acid, such as a vector. Nucleic acids may be single-stranded or double-stranded, and may include RNA and / or DNA nucleotides, as well as artificial variants thereof (e.g., peptide nucleic acids).

[0079] Nucleic acids encoding the polypeptides (e.g., heavy or light chains, variable domains only, or full length) may be isolated from B cells of mice immunized with the antigen. Nucleic acids may be isolated by conventional procedures such as polymerase chain reaction (PCR).

[0080] Nucleic acid sequences encoding the heavy and light chain variable regions are included herein. One of skill in the art will appreciate that due to the degeneracy of the genetic code, each of the polypeptide sequences disclosed herein is encoded by a multitude of other nucleic acid sequences. The present invention provides each degenerate nucleotide sequence encoding each binding construct of the present invention.

[0081] The present invention further provides nucleic acids that hybridize to other nucleic acids under specific hybridization conditions. Methods for hybridizing nucleic acids are well known in the art. For example, see Current Protocols in Molecular Biology, John Wiley & Sons, NY 1989; 6.3.1-6.3.6. As defined herein, for example, moderately stringent hybridization conditions use a pre-wash solution containing 5x sodium chloride / sodium citrate (SSC), 0.5% SDS, 1.0 mM EDTA (pH 8.0), a hybridization buffer of about 50% formamide, 6x SSC, and a hybridization temperature of 55°C (or other similar hybridization solutions, such as those containing about 50% formamide, with a hybridization temperature of 42°C), and washing conditions at 60°C in 0.5x SSC, 0.1% SDS. Stringent hybridization conditions include hybridization in 6×SSC at 45° C., followed by one or more washes in 0.1×SSC, 0.2% SDS at 68° C. Moreover, one of skill in the art can manipulate the hybridization and / or washing conditions to increase or decrease the stringency of hybridization such that nucleic acids containing nucleotide sequences that are at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98% or 99% identical to each other typically remain hybridized to each other.The basic parameters influencing the selection of hybridization conditions and guidance for devising suitable conditions are described, for example, in Sambrook, Fritsch, and Maniatis (1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, chapters 9 and 11; and Current Protocols in Molecular Biology, 1995, Ausubel et al., eds., John Wiley & Sons, Inc., sections 2.10 and 6.3-6.4) and can be readily determined by one of skill in the art, for example, based on the length and / or base composition of the DNA. Changes can be introduced by mutagenesis into the nucleic acid, which results in an alteration of the amino acid sequence of the polypeptide (e.g., binding construct) that the nucleic acid encodes. Mutations can be introduced using any technique known in the art. In one embodiment, one or more specific amino acid residues are altered, for example, using a site-directed mutagenesis protocol. In another embodiment, one or more randomly selected residues are altered, for example, using a random mutagenesis protocol. However performed, the mutant polypeptide can be expressed and screened for desirable properties.

[0082] Mutations can be introduced into the nucleic acid without significantly altering the biological activity of the polypeptide it encodes. For example, nucleotide substitutions can be made that result in amino acid substitutions at non-essential amino acid residue positions. In one embodiment, the nucleotide sequence provided herein for the binding construct of the invention, or a desired fragment, variant, or derivative thereof, encodes an amino acid sequence that includes one or more deletions or substitutions of the amino acid residues shown herein for the light chain of the binding construct of the invention or the heavy chain of the binding construct of the invention, and is mutated such that two or more sequences are different residues. In another embodiment, the mutagenesis inserts an amino acid adjacent to one or more amino acid residues shown herein for the light chain of the binding construct of the invention or the heavy chain of the binding construct of the invention, such that two or more sequences are different residues. Alternatively, one or more mutations can be introduced into the nucleic acid that selectively alter the biological activity of the polypeptide it encodes.

[0083] In another embodiment, the present invention provides a vector comprising a nucleic acid encoding a polypeptide of the present invention or a portion thereof. Examples of vectors include, but are not limited to, plasmids, viral vectors, non-episomal mammalian vectors and expression vectors, such as recombinant expression vectors.

[0084] A recombinant expression vector of the invention may contain a nucleic acid of the invention in a form suitable for expressing the nucleic acid in a host cell. The recombinant expression vector contains one or more regulatory sequences selected based on the host cell to be used for expression, and such regulatory sequences are operably linked to the nucleic acid sequence to be expressed. Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cells (e.g., the SV40 early gene enhancer, the Rous sarcoma virus promoter, and the cytomegalovirus promoter), those that direct expression of a nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences; see Voss et al., 1986, Trends Biochem. Sci. 11:287, Maniatis et al., 1987, Science 11:287, which are incorporated herein by reference in their entireties). 236:1237), as well as those that induce inducible expression of a nucleotide sequence in response to a particular treatment or condition (e.g., the metallothionein promoter in mammalian cells and the tet- and / or streptomycin-responsive promoters in both prokaryotic and eukaryotic systems, see ibid. Those of skill in the art will appreciate that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, and the like. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides encoded by nucleic acids as described herein.

[0085] In another embodiment, the present invention provides a host cell into which a recombinant expression vector of the present invention is introduced. The host cell may be any prokaryotic or eukaryotic cell. Prokaryotic host cells include gram-negative or gram-positive microorganisms, such as E. coli or bacilli. Higher eukaryotic cells include insect cells, yeast cells, and cell lines of mammalian origin. Examples of suitable mammalian host cell lines include Chinese Hamster Ovary (CHO) cells or their derivatives, such as Veggie CHO and related cell lines that grow in serum-free medium (see Rasmussen et al., 1998, Cytotechnology 28:31), or the DHFR-deficient CHO line DXB-11 (see Urlaub et al., 1980, Proc. Natl. Acad. Sci. USA 77:4216-20). Additional CHO cell lines include CHO-K1 (ATCC#CCL-61), EM9 (ATCC#CRL-1861), and UV20 (ATCC#CRL-1862). Additional host cells include the COS-7 line of monkey kidney cells (ATCC CRL 1651) (see Gluzman et al., 1981, Cell 23:175), L cells, C127 cells, 3T3 cells (ATCC CCL 163), AM-1 / D cells (described in U.S. Pat. No. 6,210,924), HeLa cells, the BHK (ATCC CRL 10) cell line, the CV1 / EBNA cell line (ATCC CCL 70) derived from the African green monkey kidney cell line CV1 (see McMahan et al., 1991, EMBO J. 10:2821), 293, 293 EBNA or MSR cells. Examples of such cells include human embryonic kidney cells such as 293, human epithelial A431 cells, human Colo205 cells, other transformed primate cell lines, normal diploid cells, cell lines derived from in vitro culture of primary tissues, primary explants, HL-60 cells, U937 cells, HaK cells or Jurkat cells.Cloning and expression vectors suitable for use with bacterial, fungal, yeast, and mammalian cell hosts are described in Pouwels et al. (Cloning Vectors: A Laboratory Manual, Elsevier, New York, 1985).

[0086] Vector DNA can be introduced into prokaryotic or eukaryotic cells by conventional transformation or transfection techniques. In the case of stable transfection of mammalian cells, it is known that only a small percentage of cells can integrate the foreign DNA into their genome, depending on the expression vector and transfection technique used. To identify and select such integrants, a gene encoding a selection marker (e.g., for antibiotic resistance) is generally introduced into the host cells together with the gene of interest. Additional selection markers include those that confer resistance to drugs such as G418, hygromycin, and methotrexate. Cells that have been stably transfected with the introduced nucleic acid can be identified by drug selection, among other methods (e.g., cells that have integrated the selection marker gene will survive, while other cells die).

[0087] The transformed cells can be cultured under conditions that promote expression of the polypeptide, which can be recovered by conventional protein purification procedures. Polypeptides contemplated for use herein include substantially homogeneous recombinant mammalian polypeptides that are substantially free from contaminating endogenous materials.

[0088] Cells containing nucleic acid encoding a bispecific binding construct of the invention also include hybridomas, the production and culture of which is described herein.

[0089] In some embodiments, a vector is provided that comprises a nucleic acid molecule as described herein. In some embodiments, the invention includes a host cell that comprises a nucleic acid molecule as described herein.

[0090] In some embodiments, a nucleic acid molecule is provided that encodes a bispecific binding construct as described herein.

[0091] In some embodiments, pharmaceutical compositions are provided comprising at least one bispecific binding construct as described herein.

[0092] Generation method The binding constructs of the present invention can be produced by any method known in the art for the synthesis of proteins (eg antibodies), in particular chemical synthesis techniques, or preferably recombinant expression techniques.

[0093] Recombinant expression of the binding construct requires constructing an expression vector that contains a polynucleotide encoding the bispecific binding construct. Once the polynucleotide encoding the bispecific binding construct is obtained, a vector for producing the bispecific binding construct can be produced by recombinant DNA techniques. The expression vector is constructed to contain the coding sequence of the bispecific binding construct and appropriate transcriptional and translational control signals. These methods include, for example, in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination.

[0094] The expression vector is transferred to a host cell by conventional techniques and the transfected cells are then cultured by conventional techniques to produce a bispecific binding construct of the invention.

[0095] A variety of host-expression vector systems may be utilized to express the bispecific binding constructs of the present invention. Such host-expression systems refer not only to the medium in which the coding sequence of interest can be produced and subsequently purified, but also to cells capable of expressing the molecules of the present invention in situ when transformed or transfected with the appropriate nucleotide coding sequence. Bacterial cells such as Escherichia coli (E. coli), and eukaryotic cells are commonly used for the expression of recombinant binding molecules, particularly whole recombinant binding molecules. For example, mammalian cells such as Chinese hamster ovary cells (CHO) in combination with vectors such as the promoter element of the major intermediate-early gene from human cytomegalovirus are effective expression systems for antibodies (Foecking et al., Gene 45:101 (1986); Cockett et al., Bio / Technology 8:2 (1990)).

[0096] In addition, a host cell line may be selected that modulates the expression of the inserted sequence or modifies and processes the gene product in the specific manner desired. Such modifications (e.g., glycosylation) and processing (e.g., cleavage) of protein products are important for the function of the protein. Different host cells have characteristic and specific mechanisms for post-translational processing and modification of proteins and gene products. Appropriate cell lines or host systems can be selected to ensure correct modification and processing of the expressed foreign protein. To this end, eukaryotic host cells that possess the cellular machinery for proper processing of the primary transcript, glycosylation, and phosphorylation of the gene product can be used. Such mammalian host cells include, but are not limited to, CHO, COS, 293, 3T3, or myeloma cells.

[0097] For long-term, high-yield production of recombinant proteins, stable expression is preferred. For example, cell lines may be engineered to stably express the binding molecule. Instead of using expression vectors containing viral origins of replication, host cells may be transformed with DNA regulated by appropriate expression control elements (e.g., promoters, enhancer sequences, transcription terminators, polyadenylation sites, etc.) and a selection marker. After introduction of the foreign DNA, the engineered cells may be grown in enriched medium for 1-2 days and then switched to selective medium. The selection marker in the recombinant plasmid confers resistance to selection, allowing the cells to stably integrate the plasmid into their chromosomes and grow to form accumulations that can be cloned and expanded into cell lines. Using this method, cell lines expressing the binding molecule may be advantageously engineered. Such engineered cell lines may be particularly useful in screening and evaluating compounds that interact directly or indirectly with the binding molecule.

[0098] A number of selection systems may be used, including, but not limited to, herpes simplex virus thymidine kinase (Wigler et al., Cell 11:223 (1977)), hypoxanthine-guanine phosphoribosyltransferase (Szybalska & Szybalski, Proc. Natl. Acad. Sci. USA 48:202 (1992)), and adenine phosphoribosyltransferase (Lowy et al., Cell 22:817 (1980)), and the genes may be used in tk, hgprt or aprt cells, respectively. Antimetabolite resistance may also be used as a criterion for selection of the following genes: dhfr, which confers resistance to methotrexate (Wigler et al., Proc. Natl. Acad. Sci. USA 77:357 (1980); O'Hare et al., Proc. Natl. Acad. Sci. USA 78:1527 (1981)); gpt, which confers resistance to mycophenolic acid (Mulligan & Berg, Proc. Natl. Acad. Sci. USA 78:2072 (1981)); neo, which confers resistance to the aminoglycoside G-418 (Wu and Wu, Biotherapy 3:87-95 (1991)); and hygro, which confers resistance to hygromycin (Santerre et al., Gene 30:147 (1984)).The desired recombinant clone may be selected by routine application of methods generally known in the art of recombinant DNA technology, such as those described, for example, in Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, NY (1993); Kriegler, Gene Transfer and Expression, A Laboratory Manual, Stockton Press, NY (1990); and in Chapters 12 and 13, Dracopoli et al. (eds), Current Protocols in Human Genetics, John Wiley & Sons, NY (1994); Colberre-Garapin et al., J. Mol. Biol. 150:1 (1981), which are incorporated herein by reference in their entireties.

[0099] The expression level of the binding molecule can be increased by amplifying the vector (for a review, see Bebbington and Hentschel, "The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells" (DNA Cloning, Vol. 3. Academic Press, New York, 1987)). If the marker in the vector system expressing the binding is amplifiable, increasing the level of inhibitor present in the host cell culture will increase the copy number of the marker gene. Since the amplified region is associated with the gene, the protein product will also increase (Crouse et al., Mol. Cell. Biol. 3:257 (1983)).

[0100] A host cell may be co-transfected with multiple expression vectors of the invention. The vectors may contain identical selectable markers that allow for similar expression of the expressed polypeptides. Alternatively, a single vector may be used that is capable of encoding and expressing, for example, the polypeptides of the invention. The coding sequence may comprise cDNA or genomic DNA.

[0101] Once the binding molecules of the invention are produced by an animal, by chemical synthesis, or expressed recombinantly, they may be purified by any method known in the art for purifying immunoglobulin molecules, such as chromatography (e.g., ion exchange chromatography, affinity chromatography, particularly affinity chromatography for specific antigens following Protein A, and size exclusion chromatography), centrifugation, differential solubility, or any other standard technique for purifying proteins. In addition, the binding constructs of the invention or fragments thereof can be fused to heterologous polypeptide sequences described herein or otherwise known in the art to facilitate purification. Purification techniques may vary depending on whether an Fc region (e.g., scFC) is attached to the bispecific binding construct of the invention.

[0102] In some embodiments, the present invention encompasses binding constructs that are recombinantly fused to a polypeptide or chemically conjugated to a polypeptide (including both covalent and non-covalent conjugates). The fused or conjugated binding constructs of the present invention may be used to facilitate purification. See, for example, Harbor et al., supra, and PCT Publication WO 93 / 21232; EP 439,095; Naramura et al., Immunol. Lett. 39:91-99 (1994); U.S. Pat. No. 5,474,981; Gillies et al., Proc. Natl. Acad. Sci. 89:1428-1432 (1992); Fell et al., J. Immunol. 146:2446-2452 (1991).

[0103] Furthermore, the binding constructs of the present invention or fragments thereof can be fused to a marker sequence, such as a peptide, to facilitate purification. In a preferred embodiment, the amino acid sequence of the marker is a hexahistidine peptide (SEQ ID NO: 58), such as the tag provided in the pQE vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, Calif., 91311), many of which are commercially available. For example, hexahistidine (SEQ ID NO: 58) provides for easy purification of the fusion protein, as described in Gentz ​​et al., Proc. Natl. Acad. Sci. USA 86:821-824 (1989). Other peptide tags useful for purification include, but are not limited to, the "HA" tag, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., Cell 37:767 (1984)), and the "flag" tag.

[0104] Generation of bispecific binding constructs The bispecific binding constructs of the invention are generally constructed by selecting the VH and VL regions from the desired antibodies and linking them using a polypeptide linker as described herein to form an HHLL bispecific binding construct, optionally with an Fc region. More specifically, nucleic acids encoding the VH, VL and linker, and optionally the Fc, are combined to generate an HHLL nucleic acid construct encoding the bispecific binding construct of the invention.

[0105] Generation of antibodies In certain embodiments, prior to generating the bispecific binding constructs of the invention, monospecific antibodies with binding specificity for the desired target are first generated.

[0106] Antibodies useful for generating the bispecific binding constructs of the present invention may be prepared by techniques well known in the art. For example, they may be prepared by immunizing an animal (e.g., a mouse or rat or rabbit) and then immortalizing the spleen cells taken from the animal after the completion of the immunization schedule. The spleen cells may be immortalized using any technique known in the art, for example, by fusing the spleen cells with myeloma cells to generate hybridomas. See, for example, Antibodies; Harlow and Lane, Cold Spring Harbor Laboratory Press, 1st Edition (e.g., from 1988) or 2nd Edition (e.g., from 2014).

[0107] In one embodiment, a humanized monoclonal antibody comprises a variable domain of a mouse antibody (or all or a portion of its antigen-binding site) and a constant domain from a human antibody. Alternatively, a humanized antibody fragment may comprise an antigen-binding site of a mouse monoclonal antibody and a variable domain fragment from a human antibody (which has lost its antigen-binding site). Procedures for generating engineered monoclonal antibodies include those described in Riechmann et al., 1988, Nature 332:323; Liu et al., 1987, Proc. Nat. Acad. Sci. USA 84:3439; Larrick et al., 1989, Bio / Technology 7:934; and Winter et al., 1993, TIPS 14:139. In one embodiment, the chimeric antibody is a CDR-grafted antibody. Techniques for humanizing antibodies are described, for example, in U.S. Patent Nos. 5,869,619; 5,225,539; 5,821,337; 5,859,205; al.,2000,J.Immunol.164:1432-41,Zhang,W.,et al.,Molecular Immunology.42(12):1445-1451,2005;Hwang W.et al.,Methods.36(1):35-42,2005;Dall'Acqua WF,et al.,Methods 36(1):43-60, 2005; and Clark, M., Immunology Today. 21(8):397-402, 2000.

[0108] The binding molecules of the invention may also comprise regions of fully human monoclonal antibodies. Fully human monoclonal antibodies may be generated by a number of techniques with which one of skill in the art would be familiar. Such methods include, but are not limited to, Epstein-Barr Virus (EBV) transformation of human peripheral blood cells (including, for example, B lymphocytes), in vitro immunization of human B cells, fusion of spleen cells from immunized transgenic mice carrying inserted human immunoglobulin genes, isolation of human immunoglobulin V regions from a phage library, or other procedures as known in the art and based on those disclosed herein.

[0109] Procedures have been developed for producing human monoclonal antibodies in non-human animals. For example, mice have been produced in which one or more endogenous immunoglobulin genes have been inactivated by various means. Human immunoglobulin genes have been introduced into mice to replace the inactivated mouse genes. In this approach, elements of human heavy and light chain loci are introduced into mouse strains derived from embryonic stem cells, which includes targeted disruption of the endogenous heavy and light chain loci (see also Bruggemann et al., Curr. Opin. Biotechnol. 8:455-58 (1997)). For example, the human immunoglobulin transgenes can be minigene constructs or transgene loci on yeast artificial chromosomes that have undergone DNA rearrangements and hypermutations specific to B cells of mouse lymphoid tissues.

[0110] The antibodies produced in the animal incorporate human immunoglobulin polypeptide chains encoded by the human genetic material introduced into the animal. In one embodiment, a non-human animal, such as a transgenic mouse, is immunized with a suitable immunogen.

[0111] Examples of techniques for producing and using transgenic animals to produce human or partially human antibodies are described in U.S. Pat. Nos. 5,814,318, 5,569,825, and 5,545,806; Davis et al., Production of human antibodies from transgenic mice in Lo, ed. Antibody Engineering: Methods and Protocols, Humana Press, NJ: 191-200 (2003); Kellermann et al., 2002, Curr Opin Biotechnol. 13: 593-97; Russell et al., 2000, Infect Immun. 68: 1820-26; Gallo et al., 2000, Eur J Immun. 30: 534-40; Davis et al., 1999, Cancer Metastasis Rev. 18: 421-25; Green, 1999, J. Immun. 1999, ... Immunol Methods.231:11-23,Jakobovits,1998,Advanced Drug Delivery Reviews 31:33-42,Green et al.,1998,J Exp Med.188:483-95,Jakobovits A,1998,Exp.Opin.Invest.Drugs.7:607-14,Tsuda et al. al.,1997,Genomics.42:413-21,Mendez et al.,1997,Nat Genet.15:146-56,Jakobovits,1994,Curr Biol.4:761-63,Arbones et al.,1994,Immunity.1:247-60,Green et al.,1994,Nat Genet.7:13-21,Jakobovits et. al.,1993,Nature.362:255-58,Jakobovits et al.,1993,Proc Natl Acad Sci US A.90:2551-55.Chen, J., M. Trounstine, FW Alt, F. Young, C. Kurahara, J. Loring, D. Huszar."Immunoglobulin gene rearrangement in B-cell deficient mice generated by targeted deletion of the JH locus." International Immunology 5(1993):647-656, Choi et al., 1993, Nature Genetics 4:117-23, Fishwild et al., 1996, Nature Biotechnology 14:845-51, Harding et al., 1995, Annals of the New York Academy of Sciences, Lonberg et al., 1994, Nature 368:856-59, Lonberg, 1994, Transgenic Approaches to Human Monoclonal Antibodies in Handbook of Experimental Pharmacology 113:49-101, Lonberg et al., 1995, Internal Review of Immunology 13:65-93, Neuberger, 1996, Nature Biotechnology 14:826, Taylor et al., 1992, Nucleic Acids Research 20:6287-95, Taylor et al., 1994, International Immunology 6:579-91, Tomizuka et al., 1997, Nature Genetics 16:133-43, Tomizuka et al., 2000, Proceedings of the National Academy of Sciences USA 97:722-27, Tuaillon et al., 1993, Proceedings of the National Academy of Sciences USA 90:3720-24, and Tuaillon et al., 1994, Journal of Immunology 152:2912-20.; Lonberg et al., Nature 368:856, 1994; Taylor et al., Int. Immun.6:579, 1994; U.S. Patent No. 5,877,397; Bruggemann et al., 1997 Curr. Opin. Biotechnol. 8:455-58; Jakobovits et al., 1995 Ann. NY Acad. Sci. 764:525-35. Additionally, protocols involving the XenoMouse® (Abgenix, now Amgen, Inc.) are described, for example, in U.S. Patent Application Serial No. 05 / 0118643, and PCT Publication Nos. WO 05 / 694879, WO 98 / 24838, WO 00 / 76310, and U.S. Patent No. 7,064,244.

[0112] For example, lymphoid cells from immunized transgenic mice are fused with myeloma cells to generate hybridomas. The myeloma cells used in the fusion procedure to generate hybridomas are preferably non-antibody producing cells, have high fusion efficiency, and have enzyme deficiencies that make them unable to grow in a specific selection medium that supports the growth of only the desired fused cells (hybridomas). Examples of cell lines suitable for use in such fusions include Sp-20, P3-X63 / Ag8, P3-X63-Ag8.653, NS1 / 1.Ag4 1, Sp210-Ag14, FO, NSO / U, MPC-11, MPC11-X45-GTG1.7, and S194 / 5XXO Bul, and examples of cell lines used in rat fusions include R210.RCY3, Y3-Ag1.2.3, IR983F, and 4B210. Other cell lines useful for cell fusion are U-266, GM1500-GRG2, LICR-LON-HMy2 and UC729-6.

[0113] Lymphoid (e.g., splenic) cells and myeloma cells may be mixed with a membrane fusion promoter, such as polyethylene glycol or a non-ionic detergent, for a few minutes and then plated at low density in a selective medium that supports the growth of hybridoma cells but does not fuse the myeloma cells. One selective medium is HAT (hypoxanthine, aminopterin, thymidine). After a sufficient amount of time, usually about 1-2 weeks, colonies of cells are observed. Single colonies may be isolated and antibodies produced by the cells may be tested for binding activity to the desired target using any one of a variety of immunoassays known in the art and described herein. The hybridomas may be cloned (e.g., by limiting dilution cloning or soft agar plaque isolation) and positive clones that produce molecules specific to the desired target are selected and cultured. Binding molecules from the hybridoma culture may be isolated from the supernatant of the hybridoma culture. Thus, the present invention provides a hybridoma comprising a polynucleotide encoding a binding construct of the present invention in the chromosome of the cell. These hybridomas can be cultured according to the methods described herein and known in the art.

[0114] Another method for generating human antibodies useful for generating the bispecific binding molecules of the present invention includes immortalizing human peripheral blood cells by EBV transformation. See, for example, U.S. Pat. No. 4,464,456. Such immortalized B cell lines (or lymphoblastoid cell lines) that produce monoclonal antibodies that specifically bind to the desired target can be identified by immunodetection methods such as those provided herein, e.g., ELISA, and then isolated by standard cloning techniques. The stability of the antibody-producing lymphoblastoid cell lines can be improved by fusing the transformed cells with mouse myeloma to generate mouse-human hybrid cell lines, according to methods known in the art (see, for example, Glasky et al., Hybridoma 8:377-89 (1989)). Yet another method for generating human monoclonal antibodies involves in vitro immunization, which involves priming human splenic B cells with an antigen and then fusing the primed B cells with a heterohybrid fusion partner. See, e.g., Boerner et al., 1991 J. Immunol. 147:86-95.

[0115] In certain embodiments, B cells producing the desired antibody are selected and the light and heavy chain variable regions are cloned from the B cells according to molecular biology techniques known in the art (WO 92 / 02551; U.S. Pat. No. 5,627,052; Babcook et al., Proc. Natl. Acad. Sci. USA 93:7843-48 (1996)) and described herein. B cells from immunized animals may be isolated from spleens, lymph nodes, or peripheral blood samples by selecting cells producing the desired antibody. B cells may also be isolated from humans, for example, from peripheral blood samples. Methods for detecting single B cells producing antibodies with the desired specificity are well known in the art, such as by plaque formation, fluorescence activated cell sorting, detection of specific antibodies after in vitro stimulation, and the like. Methods for selecting B cells producing specific antibodies include, for example, preparing a single cell suspension of B cells in soft agar containing the antigen. Binding of the specific antibody produced by the B cells to the antigen results in the formation of a complex that may be visible as an immunoprecipitate. After selection of the B cells producing the desired antibody, the specific antibody gene may be cloned by isolating and amplifying the DNA or mRNA and used to generate the bispecific binding construct of the invention according to methods known in the art and described herein.

[0116] A further method for obtaining antibodies useful for generating the bispecific binding constructs of the invention is by phage display. See, e.g., Winter et al., 1994 Annu. Rev. Immunol. 12:433-55; Burton et al., 1994 Adv. Immunol. 57:191-280. Combinatorial libraries of human or mouse immunoglobulin variable region genes may be generated in phage vectors, which can be screened to select Ig fragments (Fab, Fv, sFv, or multimers thereof) that specifically bind to a TGF-beta binding protein or variant or fragment thereof. See, e.g., U.S. Pat. No. 5,223,409; Huse et al., 1989 Science 246:1275-81; Sastry et al., Proc. Natl. Acad. Sci. USA 86:5728-32 (1989); Alting-Mees et al., Strategies in Molecular Biology 3:1-9 (1990); Kang et al., 1991 Proc. Natl. Acad. Sci. USA 88:4363-66; Hoogenboom et al., 1992 J. Molec. Biol. 227:381-388; Schlebusch et al., 1997 Hybridoma 16:47-52 and references cited therein. For example, a phage comprising multiple polynucleotide sequences encoding Ig variable region fragments may be inserted in frame with sequences encoding a phage coat protein into the genome of a filamentous bacteriophage such as M13 or a mutant thereof. The fusion protein may be a fusion of the coat protein with a light chain variable region domain and / or a heavy chain variable region domain. According to certain embodiments, immunoglobulin Fab fragments may also be displayed on a phage particle (see, e.g., U.S. Pat. No. 5,698,426).

[0117] Heavy and light chain immunoglobulin cDNA expression libraries can also be prepared in lambda phages, such as the lambda ImmunoZap™ (H) and lambda ImmunoZap™ (L) vectors (Stratagene, La Jolla, California). Briefly, mRNA is isolated from a B cell population and used to generate heavy and light chain immunoglobulin cDNA expression libraries in the lambda ImmunoZap(H) and lambda ImmunoZap(L) vectors. These vectors can be screened individually or co-expressed to form Fab fragments or antibodies (see Huse et al., supra; see also Sastry et al., supra). Positive plaques can then be converted to non-lytic plasmids that allow high level expression of monoclonal antibody fragments from E. coli.

[0118] In one embodiment, nucleotide primers are used to amplify the variable regions of the genes expressing the monoclonal antibody of interest in a hybridoma. These primers may be synthesized by one of skill in the art or purchased from a commercial source. (See, for example, Stratagene (La Jolla, California), which sells mouse and human variable region primers, including primers for the VHa, VHb, VHc, VHd, CH1, VL and CL regions, among others.) These primers may be used to amplify the heavy or light chain variable regions, which may then be inserted into vectors such as ImmunoZAP™ H or ImmunoZAP™ L (Stratagene), respectively. These vectors may then be introduced into E. coli, yeast, or mammalian-based systems for expression. Using these methods, large amounts of single-chain proteins containing fusions of the VH and VL domains can be produced (see Bird et al., Science 242:423-426, 1988).

[0119] In certain embodiments, the binding constructs of the invention may be obtained from transgenic animals (e.g., mice) producing "heavy chain only" antibodies, or "HCAbs." HCAbs are similar to naturally occurring single chain VHH antibodies of camels and llamas. See, for example, U.S. Pat. Nos. 8,507,748 and 8,502,014, as well as U.S. Pat. Appl. Pub. Nos. 2009 / 0285805A1, 2009 / 0169548A1, 2009 / 0307787A1, 2011 / 0314563A1, 2012 / 0151610A1, WO 2008 / 122886A2, and WO 2009 / 013620A2.

[0120] Once cells producing an antibody according to the invention have been obtained using any of the above mentioned immunization and other techniques, the specific antibody gene may be cloned therefrom by isolating and amplifying the DNA or mRNA according to standard procedures as described herein, and may then be used to generate a bispecific construct of the invention. The antibodies produced therefrom may be sequenced, the CDRs identified, and the DNA encoding the CDRs manipulated as described above to generate other bispecific constructs according to the invention.

[0121] Molecular evolution of the complementarity determining regions (CDRs) in the center of the antibody binding site has also been used to isolate antibodies with increased affinity, such as those described in Schier et al., 1996, J. Mol. Biol. 263:551. Such techniques are therefore useful for preparing the binding constructs of the invention.

[0122] While human, partially human or humanized antibodies are suitable for many applications, particularly for the present invention, other types of binding constructs are suitable for certain applications. These non-human antibodies may be derived, for example, from any antibody-producing animal, for example, mouse, rat, rabbit, goat, donkey, or monkey, such as a non-human primate (e.g., cynomolgus or rhesus monkey) or ape (e.g., chimpanzee). Antibodies from a particular species can be generated, for example, by immunizing an animal of that species with the desired immunogen, or by using an artificial system for generating antibodies of that species (e.g., bacterial or phage display-based systems for generating antibodies of a particular species), or by converting an antibody from one species into an antibody from another species, for example, by replacing the constant region of the antibody with a constant region from the other species, or by replacing one or more amino acid residues of the antibody to more closely resemble the sequence of an antibody from the other species. In one embodiment, the antibody is a chimeric antibody, comprising amino acid sequences derived from antibodies from two or more different species. The desired binding region sequences can then be used to generate the bispecific binding constructs of the present invention.

[0123] If it is desired to improve the affinity of a binding construct according to the invention, which contains one or more of the above mentioned CDRs, various methods can be used to improve the affinity of the binding construct, including the maintenance of the CDRs (Yang et al., J. Mol. Biol., 254, 392-403, 1995), chain shuffling methods (Marks et al., Bio / Technology, 10, 779-783, 1992), the use of mutant strains of E. coli (Low et al., J. Mol. Biol., 250, 350-368, 1996), DNA shuffling methods (Patten et al., Curr. Opin. Biotechnol., 8, 724-733, 1997), phage display methods (Thompson et al., J. Mol. Biol., 256, 7-88, 1996), and further PCR techniques (Crameri, et al., J. Mol. Biol., 256, 7-88, 1996). This can be accomplished by a number of affinity maturation protocols, including those described in Vaughan et al. (Nature Biotechnology, 16, 535-539, 1998), all of which are described in Vaughan et al. (Nature Biotechnology, 16, 535-539, 1998).

[0124] In certain embodiments, to generate the HHLL bispecific binding constructs of the invention, it may be desirable to first generate a more typical single-chain antibody, which can be formed by linking heavy and light chain variable domain (Fv region) fragments via an amino acid bridge (short peptide linker) to give a single chain polypeptide. Such single-chain Fvs (scFvs) are prepared by fusing DNA encoding a peptide linker between the DNA encoding the two variable domain polypeptides (VL and VH). The resulting polypeptides can refold on themselves to form antigen-binding monomers or multimers (e.g., dimers, trimers, or tetramers) depending on the length of the flexible linker between the two variable domains (Kortt et al., 1997, Prot. Eng. 10:423; Kortt et al., 2001, Biomol. Eng. 18:95-108). Techniques that have been developed to produce single chain antibodies include those described in U.S. Patent No. 4,946,778; Bird, 1988, Science 242:423; Huston et al., 1988, Proc. Natl. Acad. Sci. USA 85:5879; Ward et al., 1989, Nature 334:544, de Graaf et al., 2002, Methods Mol Biol. 178:379-87. These single chain antibodies are distinct and different from the bispecific binding constructs of the present invention.

[0125] Antigen-binding fragments derived from antibodies can also be obtained according to conventional methods, for example by proteolytic hydrolysis of the antibody, for example by pepsin or papain digestion of the whole antibody. As an example, antibody fragments can be generated by enzymatic cleavage of the antibody with pepsin to provide a 5S fragment designated F(ab')2. This fragment can be further cleaved using a thiol reducing agent to generate a 3.5S Fab' monovalent fragment. Optionally, the cleavage reaction can be carried out using a blocking group for the sulfhydryl groups resulting from cleavage of disulfide bonds. Alternatively, enzymatic cleavage with papain directly generates two monovalent Fab fragments and an Fc fragment. These methods are described, for example, in Goldenberg, U.S. Pat. No. 4,331,647, Nisonoff et al., Arch. Biochem. Biophys. 89:230, 1960; Porter, Biochem. J. 73:119, 1959; Edelman et al., in Methods in Enzymology 1:422 (Academic Press 1967); and by Andrews, S M and Titus, J A in Current Protocols in Immunology (Coligan J E, et al., eds), John Wiley & Sons, New York (2003), pages 2.8.1-2.8.10 and 2.10A.1-2.10A.5. Other methods for cleaving antibodies, such as separating heavy chains to form monovalent heavy-light chain fragments (Fd), further cleaving the fragments, or other enzymatic, chemical, or genetic methods may also be used so long as the fragment binds to an antigen recognized by the intact antibody.

[0126] In certain embodiments, the bispecific binding construct comprises one or more complementarity determining regions (CDRs) of an antibody. The CDRs can be obtained by constructing polynucleotides encoding the CDRs of interest. Such polynucleotides are prepared, for example, by using the polymerase chain reaction to synthesize the variable region using mRNA from an antibody-producing cell as a template (see, for example, Larrick et al., Methods: A Companion to Methods in Enzymology 2:106, 1991; Courtenay-Luck, "Genetic Manipulation of Monoclonal Antibodies," in Monoclonal Antibodies: Production, Engineering and Clinical Application, Ritter et al. (eds.), page 166 (Cambridge University Press 1995); and Ward et al., "Genetic Manipulation and Expression of Antibodies," in Monoclonal Antibodies: Principles and Applications, Birch et al., (eds.), page 137 (Wiley-Liss, Inc. 1995)). The antibody fragment may further comprise at least one variable region domain of an antibody described herein. Thus, for example, the V region domain may be a monomer and a VH or VL domain, which may comprise at least 10 -7 can independently bind to a desired target (e.g., human CD3) with an affinity equivalent to or less than M.

[0127] The variable region may be any naturally occurring variable domain or a modified version thereof. By modified version is meant a variable region that has been generated using recombinant DNA modification techniques. Such modified versions include, for example, those generated from the variable region of a specific antibody by insertions, deletions, or modifications within or to the amino acid sequence of the specific antibody. The skilled artisan can use any known method to identify suitable amino acid residues for modification. Further examples include modified variable regions that include at least one CDR and optionally one or more framework amino acids from a first antibody and the remainder of the variable region domain from a second antibody. Modified versions of antibody variable domains may be generated by a number of techniques with which the skilled artisan will be familiar.

[0128] The variable region may be covalently linked at the C-terminal amino acid to at least one other antibody domain or fragment thereof. Thus, for example, the VH present in the variable region may be linked to the CH1 domain of an immunoglobulin. Similarly, the VL domain may be linked to a CK domain. Thus, for example, the antibody may be a Fab fragment containing associated VH and VL domains, with the antigen-binding domain covalently linked to the CH1 and CK domains at the C-terminus of the VH and VL domains, respectively. The CH1 domain may be extended with further amino acids, for example to provide a hinge region or part of a hinge region domain as found in a Fab' fragment, or to provide further domains such as the CH2 and CH3 domains of an antibody.

[0129] Binding specificity The antibody or bispecific binding construct may be -7 A molecule "specifically binds" to an antigen when it binds to the antigen with high binding affinity as measured by an equilibrium dissociation constant (KD, or the equivalent of KD, as defined below) with a value equal to or less than M.

[0130] Affinity can be measured using a variety of techniques known in the art, including, but not limited to, equilibrium methods (e.g., enzyme-linked immunosorbent assay (ELISA); KinExA, Rathanaswami et al. Analytical Biochemistry, Vol. 373:52-60, 2008; or radioimmunoassay (RIA)), or surface plasmon resonance assays, or other kinetic-based assay mechanisms (e.g., BIACORE® analysis or Octet® analysis (forteBIO)), as well as other methods such as indirect binding assays, competitive binding assays, fluorescence resonance energy transfer (FRET), gel electrophoresis, and chromatography (e.g., gel filtration chromatography). These and other methods may employ a label on one or more of the components being tested and / or may employ a variety of detection methods, including, but not limited to, chromogenic, fluorescent, luminescent, or isotopic labels. A detailed description of binding affinity and kinetics can be found in Paul, WE, ed., Fundamental Immunology, 4th Ed., Lippincott-Raven, Philadelphia (1999), which focuses on antibody-immunogen interactions. One example of a competitive binding assay is a radioimmunoassay, which involves incubating a labeled antigen with an antibody of interest in the presence of increasing amounts of unlabeled antigen and detecting the antibody bound to the labeled antigen. The affinity and binding dissociation rate of the antibody of interest for a particular antigen can be determined from the data by Scatchard plot analysis. Competition with a second antibody can also be determined using a radioimmunoassay, in which the antigen is incubated with the antibody of interest conjugated to a labeled compound in the presence of increasing amounts of unlabeled second antibody. This type of assay can be easily adapted for use with the bispecific binding constructs of the present invention.

[0131] A further embodiment of the present invention is -7 Less than M or 10 -8 Less than M or 10 -9 Less than M or 10 -10Less than M or 10 -11 Less than M or 10 -12 Less than M or 10 -13 Less than M or 5 x 10 -13 In yet a further embodiment of the invention, the present invention provides a bispecific binding construct that binds to a desired target with an equilibrium dissociation constant, or KD (koff / kon), of less than about M, with lower values ​​indicating higher binding affinity. -7 Less than M or about 10 -8 Less than M or about 10 -9 Less than M or about 10 -10 Less than M or about 10 -11 Less than M or about 10 -12 Less than M or about 10 -13 Less than M or about 5 x 10 -13 A bispecific binding construct that binds to a desired target with an equilibrium dissociation constant, or KD (koff / kon), less than M.

[0132] In yet another embodiment, the bispecific binding construct that binds to a desired target is about 10 -7 M~about 10 -8 M, about 10 -8 M~about 10 -9 M, about 10 -9 M~about 10 -10 M, about 10 -10 M~about 10 -11 M, about 10 -11 M~about 10 -12 M, about 10 -12 M~about 10 -13 In yet another embodiment, the bispecific construct of the invention has an equilibrium dissociation constant, or KD(koff / kon), of 10 -7 M~10 -8 M, 10 -8 M~10 -9 M, 10 -9 M~10 -10 M, 10 -10 M~10 -11 M, 10 -11 M~10 -12 M, 10 -12 M~10 -13 M has an equilibrium dissociation constant, i.e., KD (koff / kon).

[0133] molecular stability Various aspects of molecular stability may be desired, particularly in the context of biopharmaceutical therapeutic molecules. For example, stability at various temperatures ("thermal stability") may be desired. In some embodiments, the thermal stability may include stability at physiological temperature ranges, e.g., about 37°C, or 32°C to 42°C. In other embodiments, the thermal stability may include stability at higher temperature ranges, e.g., 42°C to 60°C. In other embodiments, the thermal stability may include stability at lower temperature ranges, e.g., 20°C to 32°C. In yet other embodiments, the thermal stability may include stability in frozen conditions, e.g., at or below 0°C.

[0134] Assays for measuring the thermal stability of protein molecules are known in the art. For example, the fully automated UNcle platform (Unchained Labs) is used, which can simultaneously obtain intrinsic protein fluorescence and static light scattering (SLS) data during a thermal gradient, and is further described in the Examples. Furthermore, the thermal stability and aggregation assays described herein in the Examples, such as differential scanning fluorimetry (DSF) and static light scattering (SLS), can also be used to measure both thermal melting (Tm) and thermal aggregation (Tagg), respectively.

[0135] Alternatively, the molecule can be subjected to accelerated stress testing as described herein in the Examples. Briefly, this testing involves incubating the protein molecule at a specific temperature (e.g., 40°C) and then measuring aggregation at various time points by size exclusion chromatography (SEC), where lower aggregation levels indicate better protein stability.

[0136] Alternatively, the thermal stability parameter can be determined in terms of the temperature at which the molecule aggregates as follows: A solution of the molecule at a concentration of 250 μg / ml is transferred into a single-use cuvette and placed in a dynamic light scattering (DLS) instrument. The sample is heated from 40° C. to 70° C. at a heating rate of 0.5° C. / min while the measured radius is constantly being acquired. The increase in radius indicates the melting and aggregation of the protein and is used to calculate the aggregation temperature of the molecule.

[0137] Alternatively, melting temperature curves can be measured by differential scanning calorimetry (DSC) to measure the intrinsic biophysical protein stability of the binding construct. These experiments are performed using a MicroCal LLC (Northampton, MA, USA) VP-DSC instrument. The energy uptake of samples containing the binding construct is recorded from 20°C to 90°C and compared to samples containing only the formulation buffer. The binding construct is adjusted to a final concentration of, for example, 250 μg / ml in SEC running buffer. The overall temperature of the sample is increased stepwise to record each melting curve. The energy uptake of the sample and formulation buffer standard at each temperature T is recorded. The difference in the energy uptake Cp (kcal / mole / °C) of the sample minus the standard is plotted against each temperature. The melting temperature is defined as the temperature at which the energy uptake first reaches a maximum.

[0138] In a further embodiment, the bispecific binding construct according to the invention is stable at approximately physiological pH, i.e. about pH 7.4. In another embodiment, the bispecific binding construct is stable at a low pH, for example up to pH 6.0. In another embodiment, the bispecific binding construct is stable at a high pH, ​​for example up to pH 9.0. In one embodiment, the bispecific binding construct is stable at a pH between 6.0 and 9.0. In another embodiment, the bispecific binding construct is stable at a pH between 6.0 and 8.0. In another embodiment, the bispecific binding construct is stable at a pH between 7.0 and 9.0.

[0139] In certain embodiments, the more resistant the bispecific binding construct is to non-physiological pH (e.g., pH 6.0), the higher the recovery of molecules eluted from the ion exchange column relative to the total amount of protein loaded. In one embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧30%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧40%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧50%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧60%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧70%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧80%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧90%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧95%. In another embodiment, the recovery of molecules from the ion (e.g., cation) exchange column is ≧99%.

[0140] In certain embodiments, it may be desirable to measure the chemical stability of the molecule. Measurement of the chemical stability of the bispecific binding construct can be performed by isothermal chemical denaturation ("ICD") by monitoring intrinsic protein fluorescence as further described herein in the Examples. ICD yields C1 / 2 and ΔG, which can be good metrics for protein stability. C1 / 2 is the amount of chemical denaturant required to denature 50% of the protein, which is used to derive ΔG (i.e., the unfolding energy).

[0141] Clipping of protein chains is another critical product quality attribute closely observed or reported for biologics. Typically, longer and / or less structured linkers are expected to experience increased clipping depending on incubation time and temperature. Clipping is a significant issue for bispecific binding constructs, since clipping to the linker connecting the targeting domain or the T cell binding domain ultimately has a detrimental effect on the potency and efficacy of the drug. Clipping to additional sites, including the scFc, can affect pharmacodynamic / kinetic properties. Increased clipping is a property that must be avoided in pharmaceuticals. Thus, in certain embodiments, protein clipping can be assayed as described herein in the Examples.

[0142] Immune effector cells and effector cell proteins The bispecific binding construct can bind to a molecule expressed on the surface of an immune effector cell (herein referred to as an "effector cell protein") and to another molecule expressed on the surface of a target cell (herein referred to as a "target cell protein"). The immune effector cell can be a T cell, a NK cell, a macrophage, or a neutrophil. In some embodiments, the effector cell protein is a protein contained in the T cell receptor (TCR)-CD3 complex. The TCR-CD3 complex is a heteromultimer that includes heterodimers of TCRα and TCRβ, or TCRγ and TCRδ, as well as various CD3 chains from among the CD3 zeta (CD3ζ), CD3 epsilon (CD3ε), CD3 gamma (CD3γ), and CD3 delta (CD3δ) chains.

[0143] The CD3 receptor complex is a protein complex, composed of four chains. In mammals, this complex contains the CD3γ (gamma), CD3δ (delta) and two CD3ε (epsilon) chains. These chains associate with the T cell receptor (TCR) and the so-called ζ (zeta) chain to form the T cell receptor-CD3 complex, which generates an activation signal in T lymphocytes. The CD3γ (gamma), CD3δ (delta) and CD3ε (epsilon) chains are very closely related cell surface proteins of the immunoglobulin superfamily that contain a single extracellular immunoglobulin domain. The intracellular tail of the CD3 molecule contains a single conserved motif known as the immunoreceptor tyrosine-based activation motif, or ITAM for short, which is essential for the signaling capacity of the TCR. The CD3 epsilon molecule is a polypeptide encoded by the CD3E gene, which is present on chromosome 11 in humans. The most preferred epitope of CD3 epsilon is included in the range of amino acid residues 1-27 of the human CD3 epsilon extracellular domain. It is envisaged that the bispecific binding constructs according to the invention will typically and advantageously exhibit less undesirable non-specific T cell activation in certain immunotherapies, which in turn reduces the risk of side effects.

[0144] In some embodiments, the effector cell protein may be a human CD3 epsilon (CD3ε) chain (the mature amino acid sequence of which is disclosed in SEQ ID NO: 40), which may be part of a multimeric protein. Alternatively, the effector cell protein may be a human and / or cynomolgus TCR α, TCR β, TCR β, TCR γ, CD3 beta (CD3β) chain, CD3 gamma (CD3γ) chain, CD3 delta (CD3δ) chain, or CD3 zeta (CD3ζ) chain.

[0145] Furthermore, in some embodiments, the bispecific binding constructs may also bind to CD3ε chains from non-human species, such as mouse, rat, rabbit, New World monkey, and / or Old World monkey species. Such species include, but are not limited to, the following mammalian species: Mus musculus; Rattus rattus; Rattus norvegicus; Macaca fascicularis; Papio hamadryas; Papio papio; Papio anubis; Papio cynocephalus; Papio ursinus; Callithrix jacchus; Saguinus oedipus; and Saimiri sciureus. The mature amino acid sequence of the CD3ε chain from the cynomolgus monkey is shown in SEQ ID NO: 41. Therapeutic molecules that have comparable activity in humans and species commonly used for preclinical testing, such as mice and monkeys, can simplify and expedite drug development and ultimately lead to improved outcomes - advantages that can be significant during the lengthy and costly process of bringing a drug to market.

[0146] In a particular embodiment, the bispecific binding construct is capable of binding to an epitope within the first 27 amino acids of the CD3ε chain (SEQ ID NO: 43), which may be the human CD3ε chain or a CD3ε chain from a different species, in particular one of the mammalian species listed above. The epitope may contain the amino acid sequence Gln-Asp-Gly-Asn-Glu (SEQ ID NO: 59). The advantages of molecules that bind to such epitopes are explained in detail in US Patent Application Publication No. 2010 / 0183615A1, the relevant portions of which are incorporated herein by reference. The epitope to which an antibody or a bispecific binding construct binds can be determined, for example, by alanine scanning, as described in US Patent Application Publication No. 2010 / 0183615A1, the relevant portions of which are incorporated herein by reference. In another embodiment, the bispecific binding construct is capable of binding to an epitope within the extracellular domain of CD3ε (SEQ ID NO: 42).

[0147] In embodiments where T cells are immune effector cells, the effector cell proteins to which the bispecific binding constructs can bind include, but are not limited to, CD3ε chain, CD3γ, CD3δ chain, CD3ζ chain, TCRα, TCRβ, TCRγ, and TCRδ. In embodiments where NK cells or cytotoxic T cells are immune effector cells, the effector cell proteins can be, for example, NKG2D, CD352, NKp46, or CD16a. In embodiments where CD8+ T cells are immune effector cells, the effector cell proteins can be, for example, 4-1BB or NKG2D. Alternatively, in other embodiments, the bispecific binding constructs can bind other effector cell proteins expressed on T cells, NK cells, macrophages, or neutrophils.

[0148] Target cells and target cell proteins expressed in target cells As explained above, the bispecific binding construct can bind to an effector cell protein and a target cell protein. The target cell protein can be expressed, for example, on the surface of a cancer cell, a cell infected with a pathogen, or a cell that mediates a disease, such as an inflammatory, autoimmune, and / or fibrotic condition. In some embodiments, the target cell protein can be highly expressed on the target cell, although high levels of expression are not necessarily required.

[0149] When the target cell is a cancer cell, the bispecific binding construct as described herein can bind to a cancer cell antigen as explained above. The cancer cell antigen can be a human protein or a protein from another species. For example, the bispecific binding construct can bind to a target cell protein from mouse, rat, rabbit, New World monkey, and / or Old World monkey species, among others. Such species include, but are not limited to, the following: house mouse (Mus musculus); brown rat (Rattus rattus); brown rat (Rattus norvegicus); cynomolgus macaque (Macaca fascicularis); Hamadryas baboon (Papio hamadryas); Guinea baboon (Papio papio); Papio anubis; Papio cynocephalus; Papio ursinus; marmoset (Callithrix jacchus), cotton-top tamarin (Saguinus Oedipus), and squirrel monkey (Saimiri sciureus).

[0150] In some instances, the target cell protein may be a protein that is selectively expressed in infected cells. For example, in the case of HBV or HCV infection, the target cell protein may be an envelope protein of HBV or HCV that is expressed on the surface of infected cells. In other embodiments, the target cell protein may be gp120 encoded by human immunodeficiency virus (HIV) on HIV-infected cells.

[0151] In other embodiments, the target cells may be cells that mediate autoimmune or inflammatory diseases. For example, human eosinophils in asthma may be the target cells, in which case the target cell protein may be, for example, EGF-like module-containing mucin-like hormone receptor (EMR1). Alternatively, the target cells may be excess human B cells in systemic lupus erythematosus patients, in which case the target cell protein may be, for example, CD19 or CD20. In other autoimmune conditions, the target cells may be excess human Th2 T cells, in which case the target cell protein may be, for example, CCR4. Similarly, the target cells may be fibrotic cells that mediate diseases such as atherosclerosis, chronic obstructive pulmonary disease (COPD), cirrhosis, scleroderma, renal transplant fibrosis, renal allograft nephropathy, or pulmonary fibrosis, including idiopathic pulmonary fibrosis and / or idiopathic pulmonary hypertension. In such fibrotic conditions, the target cell protein may be, for example, fibroblast activation protein alpha (FAPα).

[0152] Therapeutic methods and compositions Bispecific binding constructs can be used to treat a wide variety of conditions including, for example, various forms of cancer, infectious diseases, autoimmune or inflammatory conditions, and / or fibrotic conditions.

[0153] Thus, in one embodiment, provided herein are bispecific binding constructs for use in preventing, treating, or ameliorating disease.

[0154] In another embodiment there is provided the use of a binding construct of the invention (or a binding construct produced according to a process of the invention) in the manufacture of a medicament for the prevention, treatment or amelioration of disease.

[0155] Pharmaceutical compositions comprising the bispecific binding constructs are provided herein. These pharmaceutical compositions comprise a therapeutically effective amount of the bispecific binding construct and one or more additional components, such as a physiologically acceptable carrier, excipient, or diluent. In some embodiments, these additional components may include buffers, carbohydrates, polyols, amino acids, chelating agents, stabilizers, and / or preservatives, among many other possibilities.

[0156] In some embodiments, bispecific binding constructs can be used to treat cell proliferative disorders including cancers involving unregulated and / or inappropriate cell proliferation, sometimes accompanied by destruction of adjacent tissue and growth of new blood vessels that allow cancer cells to invade new areas, i.e., metastasize. Conditions that can be treated by bispecific binding constructs include non-malignant conditions involving inappropriate cell proliferation, including colorectal polyps, cerebral ischemia, gross cystic disease, polycystic kidney disease, benign prostatic hyperplasia, and endometriosis. Bispecific binding constructs can be used to treat hematological or solid tumor malignancies. More specifically, cell proliferative diseases that can be treated using the bispecific binding constructs include, for example, mesothelioma, squamous cell carcinoma, myeloma, osteosarcoma, glioblastoma, glioma, carcinoma, adenocarcinoma, melanoma, sarcoma, acute and chronic leukemia, lymphoma, and meningioma, Hodgkin's disease, Sezary syndrome, multiple myeloma, and lung cancer, non-small cell lung cancer, small cell lung cancer, laryngeal cancer, breast cancer, head and neck cancer, bladder cancer, ovarian cancer. , skin cancer, prostate cancer, cervical cancer, vaginal cancer, gastric cancer, renal cell carcinoma, kidney cancer, pancreatic cancer, colorectal cancer, endometrial cancer, and esophageal cancer, hepatobiliary cancer, bone cancer, skin cancer, and blood cancer, as well as cancers of the nasal cavity and paranasal sinuses, nasopharynx, oral cavity, oropharynx, larynx, hypolarynx, salivary glands, mediastinum, stomach, small intestine, colon, rectum and anal region, ureter, urethra, penis, testes, vulva, endocrine system, central nervous system, and plasma cells.

[0157] Texts that provide guidance for cancer therapy include Cancer, Principles and Practice of Oncology, 4th Edition, DeVita et al., Eds. J.B. Lippincott Co., Philadelphia, PA (1993). Depending on the particular type of cancer and other factors such as the patient's general condition, appropriate therapeutic measures are selected as recognized in the relevant field. In treating cancer patients, bispecific binding constructs can be added to therapeutic regimens using other anti-neoplastic agents.

[0158] In some embodiments, the bispecific binding constructs can be administered simultaneously with, prior to, or subsequent to a variety of drugs and treatments commonly used in the treatment of cancer, such as, for example, chemotherapeutic agents, non-chemotherapeutic antineoplastic agents, and / or radiation. For example, chemotherapy and / or radiation can be administered prior to, during, and / or subsequent to any of the treatments described herein. Examples of chemotherapeutic agents are described above and include, but are not limited to, cisplatin, taxol, etoposide, mitoxantrone (Novantrone®), actinomycin D, cycloheximide, camptothecin (or water-soluble derivatives thereof), methotrexate, mitomycin (e.g., mitomycin C), dacarbazine (DTIC), antineoplastic antibiotics such as adriamycin (doxorubicin) and daunomycin, and all of the aforementioned chemotherapeutic agents.

[0159] The bispecific binding constructs can also be used to treat infectious diseases, such as chronic Hepatitis B virus (HBV) infection, Hepatitis C virus (HCV) infection, Human Immunodeficiency Virus (HIV) infection, Epstein-Barr virus (EBV) infection, or Cytomegalovirus (CMV) infection, among others.

[0160] Bispecific binding constructs may find further use in other types of conditions if they are useful for reducing certain cell types. For example, it may be beneficial to reduce human eosinophils in asthma, excess human B cells in systemic lupus erythematosus, excess human Th2 T cells in autoimmune conditions, or pathogen-infected cells in infectious diseases. In fibrotic conditions, it may be beneficial to reduce cells that form fibrotic tissue.

[0161] A therapeutically effective dose of the bispecific binding construct can be administered. The amount of the bispecific binding construct that constitutes a therapeutic dose may vary depending on the indication being treated, the patient's weight, and the calculated skin surface area of ​​the patient. The dosage of the bispecific binding construct can be adjusted to obtain the desired effect. Repeated administration may be required in many cases.

[0162] Bispecific binding constructs, or pharmaceutical compositions containing such molecules, can be administered by any feasible method. Protein therapeutics are usually administered parenterally, for example by injection, since oral administration, unless in a particular formulation or situation, would result in protein hydrolysis in the acidic environment of the stomach. Possible routes of administration include subcutaneous, intramuscular, intravenous, intraarterial, intralesional, or peritoneal bolus injection. Bispecific binding constructs can also be administered by injection, for example intravenous or subcutaneous. Topical administration is also possible, especially for diseases involving the skin. Alternatively, bispecific binding constructs can be administered by contact with a mucous membrane, for example by intranasal, sublingual, intravaginal, or rectal administration or as an inhalant. Alternatively, certain suitable pharmaceutical compositions containing bispecific binding constructs can be administered orally.

[0163] The term "treatment" encompasses alleviation of at least one symptom or other manifestation of a disorder, or reducing disease severity, and the like. A bispecific binding construct according to the invention need not result in a complete cure or eradication of every disease symptom or manifestation to constitute a viable therapeutic. As recognized in the relevant art, a drug used as a therapeutic can reduce the severity of a given pathology, but need not eliminate every manifestation of the disease to be considered a useful therapeutic. It is sufficient to merely reduce the impact of the disease (e.g., by reducing the number or severity of its symptoms, or by increasing the effectiveness of another treatment, or by producing another beneficial effect), or to reduce the likelihood that the disease will develop or worsen in a subject. One embodiment of the present invention relates to a method comprising administering to a patient a bispecific binding construct of the present invention in an amount and for a time sufficient to induce a sustained improvement over baseline in an index reflecting the severity of a particular disorder.

[0164] The term "prevention" includes preventing at least one symptom or other manifestation of the disorder, etc. A prophylactically administered treatment incorporating a bispecific binding construct according to the invention need not be completely effective in preventing the onset of a condition to constitute a viable prophylactic agent. It is sufficient to simply reduce the likelihood that a disease will develop or worsen in a subject.

[0165] As understood in the relevant art, pharmaceutical compositions comprising bispecific binding constructs are administered to a subject in a manner appropriate for the indication and composition. The pharmaceutical compositions may be administered by any suitable technique, including, but not limited to, parenteral administration, topical administration, or administration by inhalation. For injection, the pharmaceutical compositions may be administered, for example, via intra-articular, intravenous, intramuscular, intralesional, intraperitoneal, or subcutaneous routes, by bolus injection, or by continuous infusion. Delivery by inhalation includes, for example, nasal or oral inhalation, use of a nebulizer, inhaling the binding construct in aerosol form, and the like. Other alternatives include oral formulations, including pills, syrups, or drops.

[0166] The bispecific binding constructs can be administered in the form of a composition that includes one or more additional components, such as a physiologically acceptable carrier, excipient, or diluent. Optionally, the composition further includes one or more physiologically active agents. In various specific embodiments, the composition includes 1, 2, 3, 4, 5, or 6 physiologically active agents in addition to the one or more bispecific binding constructs.

[0167] Kits are provided for use by a physician that include one or more bispecific binding constructs and a label or other instructions for use in treating any of the conditions described herein. In one embodiment, the kit includes a sterile formulation of one or more bispecific binding constructs, which may be in the form of a composition as disclosed herein and may be contained in one or more vials.

[0168] The dosage and frequency of administration may vary depending on factors such as the route of administration, the particular bispecific binding construct used, the nature and severity of the disease being treated, whether the condition is acute or chronic, and the size and general condition of the subject.

[0169] Having described the invention in general terms above, the following examples are offered by way of illustration and not by way of limitation. EXAMPLES

[0170] Example 1 Generation and expression of bispecific HHLL-binding constructs To assess whether the proposed HHLL constructs result in enhanced stability, we designed, expressed, and purified a panel of BiTEs targeting Flt3, Msln, and Dll3, respectively, including standard, or wild-type (WT), HLE-BiTEs (HLE = extended half-life) and three different linker length constructs of the proposed HHLL BiTE structures: tight, medium, and loose.

[0171] To connect the various chains that comprise the HLE-BiTE, a linker of repeats of GlyGlyGlyGlySer (G4S) (SEQ ID NO: 1) was used. In the WT HLE-BiTE, the heavy and light chains (H1 and L1) of the targeting domain were connected by three G4S repeats (SEQ ID NO: 3). The anti-CD3 domain also contained a pair of heavy and light chains (H2 and L2), connected by three G4S repeats (SEQ ID NO: 3). A single G4S linker (SEQ ID NO: 1) was required to connect the anti-targeting domain and the anti-CD3 scFv domain. Alternatively, this can be described by the following nomenclature: H1-(G4S) 3 -L1-(G4S) 1 -H2-(G4S) 3 -L2.

[0172] In the proposed "tight" HHLL configuration, two G4S repeats (SEQ ID NO:2) connect H1 to H2, followed by three G4S repeats (SEQ ID NO:3) connecting this segment to the L1 strand. Another three G4S repeats (SEQ ID NO:3) were required to connect H1H2L1 to L2 and allow for proper folding as determined by molecular modeling. This "tight" configuration can also be written as follows: H1-(G4S)2-H2-(G4S)3-L1(G4S)3-L2, or 233 for short.

[0173] The "medium" configuration connects all of the various chains (H1, H2, L1 and L2) by a series of four repeats of G4S linkers (SEQ ID NO: 4) and can be written as follows: H1-(G4S)4-H2-(G4S)4-L1(G4S)4-L2, or 444 for short.

[0174] The "loose" construct uses a series of five repeats of the G4S linker (SEQ ID NO:5) and can be written as follows: H1-(G4S)5-H2-(G4S)-L1(G4S)5-L2, or 555 for short.

[0175] Plasmids: An expression plasmid harboring the BiTE gene of interest with a signal peptide at its N-terminus was cloned into the pTT5 vector.

[0176] Expression and purification: All BiTE proteins were produced using transiently transfected HEK293-6E cells. Briefly, plasmid DNA encoding the BiTE target sequence with a signal secretion peptide at the N-terminus was introduced into cells with a viability of approximately 99.9% and a cell density of 1.5e6 by PEI MAX transfection reagent. Protein overproduction was achieved by incubation at 37°C, 5% CO 2 The cells were then maintained at 150 RPM for 6 days, after which the cells were harvested by centrifugation (4000 RPM for 30 minutes) and the resulting cell culture supernatant was then filtered and saved for purification.

[0177] BiTEs expressed with single-chain Fc ("scFc") were purified using Protein A affinity chromatography (GE Healthcare, HiTrap mAb Select SuRe). Protein A resin was equilibrated in binding buffer (25 mM Tris, 100 mM NaCl, pH 7.4) and proteins were eluted using 100 mM sodium acetate (pH 3.6). To rapidly remove BiTEs from the elution buffer, a desalting step (GE Healthcare, HiPrep 26 / 10 Desalting) was performed in 10 mM potassium phosphate, 75 mM lysine, 4% trehalose (pH 8.0), followed by separation by size exclusion chromatography (GE Healthcare, HiLoad Superdex 200). BiTEs without scFc were purified in a similar manner using Protein L resin (GE Healthcare, HiTrap Protein L) instead of Protein A. Purity was confirmed by SDS-PAGE. The protein was then formulated at a concentration of 1 mg / ml in 10 mM glutamic acid, 9% sucrose, 0.01% polysorbate 80 (pH 4.2). The protein was stored at -80°C prior to use.

[0178] Example 2 Analytical HPLC Size Exclusion Chromatography To quantify protein aggregation and stability, several time course temperature studies were completed in conjunction with analytical size exclusion chromatography (SEC). To simplify the analysis, the same SEC method was used regardless of temperature study. Approximately 2 μg of sample was injected onto a SEC column (Waters ACQUITY UPLC Protein BEH SEC 200 Å) equilibrated in 100 mM sodium phosphate, 250 mM NaCl, pH 6.8. Absorbance data was collected at 280 nm and peaks were integrated using Chromeleon. Data was plotted and analyzed in GraphPad Prism.

[0179] Example 3 Reduced Capillary Electrophoresis To check for the formation of low molecular weight (LMW) species in the thermal stability assay, reduced capillary electrophoresis-sodium dodecyl sulfate (rCE-SDS) analysis was performed. Prior to analysis, samples were denatured and reduced by SDS and heat, and the presence of β-mercaptoethanol, respectively. Approximately 10 μg of sample was then loaded into a CE cassette, and proteins and fragments were monitored by UV detection. Purity was determined by quantifying the peak area percentage using Chromeleon.

[0180] Example 4 chemical stability Isothermal chemical denaturation Measurement of chemical stability of BiTEs is performed by isothermal chemical denaturation ("ICD") by monitoring intrinsic protein fluorescence. ICD yields C1 / 2 and ΔG, which can be good metrics for protein stability. C1 / 2 is the amount of chemical denaturant required to denature 50% of the protein, which is used to derive ΔG (i.e., unfolding energy). Intrinsic protein fluorescence was measured to monitor protein unfolding in response to chemical denaturant. Three different targets, Flt3, Msln, and Dll3, were used to investigate the chemical stability profile of HHLL constructs. The process was fully automated by using a HUNK instrument (Unchained Labs). Thirty-two independent denaturation data points were generated over the range of 0-5.52 M guanidine hydrochloride (GuHCl), and the resulting 350 / 330 nm fluorescence intensity ratios were plotted and fitted to determine the fraction denatured, deriving C1 / 2 and ΔG values. Forty-two data points were collected to define the Flt3 data for maximum robustness. The plot shows the normalized data fit. The data was fitted to either a two-state or three-state model. Figure 8 shows the results of this experiment.

[0181] The extent of improvement in chemical stability was observed to be the highest in the case of Flt3. Here, Flt3-444 and 555, i.e., the "medium" and "loose" mutants, showed a significant improvement compared to Flt3-WT, as observed by a shift in C1 / 2 of about 0.8 M and an improvement in ΔG of about 1-6 kcal / mol. On the other hand, in the case of both Msln and Dll3, no dramatic shift in C1 / 2 was observed. However, Msln-555, i.e., the loose structure, was slightly less stable than Msln-WT, as a lower ΔG value was observed. From this data, it was concluded that the chemical stability of the HHLL configuration, as observed for the Flt3-444 and Flt-555 mutants, can function comparably to the canonical HLHL configuration or, in ideal cases, surpass the WT protein in a target domain-dependent manner.

[0182] Table 3 below summarizes some of these results.

[0183] [Table 3]

[0184] Example 5 thermal stability Tm measurement by differential scanning fluorometer and Tag measurement by static light scattering To measure the various Tm and Tagg values ​​of BiTE proteins, a fully automated UNcle platform (Unchained Labs) was used that allows for the simultaneous acquisition of intrinsic protein fluorescence and static light scattering (SLS) data during a thermal gradient. Briefly, 1 mg / ml protein samples were subjected to a thermal gradient from 20 to 90°C during which data was acquired. Tm and Tagg values ​​were derived from the average of three replicates using UNcle analysis software.

[0185] Further characterization of the HHLL constructs using thermal stability and aggregation assays further revealed improved stability metrics. Results of these various experiments are shown in Figures 9A, 9B, and 10. A panel of BiTE molecules was screened using both differential scanning fluorimetry (DSF) and static light scattering (SLS) to measure both thermal melting (Tm) and thermal aggregation (Tagg), respectively. Interestingly, again, it was observed that the degree of improvement was highest for Flt3 in terms of Tm profile. In this data, all HHLL Flt3 mutants (Flt3-233, Flt3-444, and Flt3-555) showed an improvement in Tm of about 3°C, as confirmed by the DSF data and the shift in the derivative curve. Thermal gradient static light scattering data for Flt3 does not show this improvement as Flt3-444 and Flt3-555, but they have comparable Tagg values ​​compared to Flt3-WT. Notably, the tight conformation of Flt3-233 had a significant reduction in Tagg, which may indicate that this mutant may aggregate more readily compared to Flt3-444, Flt3-555, and Flt3-WT.

[0186] In the case of the HHLL variants of Msln, we did not observe any significant changes to the DSF or derivative curves, and therefore to the Tm. Interestingly, however, we did not observe any significant differences in the Tagg properties of the HHLL variants of Msln. Here, an improvement of about 2-3°C was observed in the Tagg values ​​of Msln-233, Msln-444, and Msln-555. This suggests that the HHLL variants of Msln do indeed have improved aggregation properties compared to the standard Msln BiTE. Similar trends were observed in the HHLL and standard BiTEs of Dll3. The data suggest that indeed the HHLL configuration may outperform the standard configuration.

[0187] Accelerated Stress Test The performance of HHLL constructs was evaluated under accelerated stress conditions. A panel of BiTEs was incubated at 40°C and aggregation was measured at TO, 2 weeks (2W) and 4 weeks (4W) by analytical size exclusion chromatography (SEC). Aggregation levels were quantified by integrating the high molecular weight (HMW), main and low molecular weight (LMW) peaks. Interestingly, unlike previous data where HHLL variants of Flt3 showed the greatest level of improvement in protein stability (i.e. ICD and Tm), no significant improvement in their aggregation properties was observed. However, this is consistent with the Tagg data obtained with SLS for Flt3, where no difference in Tagg was observed. It should be noted that the SLS data suggests that Flt-233, i.e. the "tight" construct, may be more prone to aggregation, as suggested by the reduced Tagg value, and this was also observed in the experimental SEC measurements. Both Msln and Dll3 HHLL mutants had improved Tagg properties, and SEC showed improved aggregation properties for Msln / Dll3-444 and Msln-555 mutants. Msln-444 and 555 BiTEs showed the greatest improvement. The 444 mutant showed half the aggregation levels compared to WT over time at 4 weeks. Interestingly, the Msln-555 mutant appeared to have fully reversible aggregation, with HMW species reduced to undetectable amounts over 4 weeks. As a negative control, the Dll3-332 HHLL mutant was included, which has a non-ideal linker length. This protein contained a short final linker that likely inhibited the trailing light chain from folding, as observed by molecular modeling. This protein aggregated more readily over time than WT or HHLL mutants, validating our molecular modeling approach. Taken together, these data suggest that HHLL constructs with optimized linker length can provide an improvement against aggregation, or are at least comparable to WT.

[0188] Protein Clipping In addition to investigating facilitating stress-induced aggregation, protein clipping was examined. Clipping is a significant issue for BiTEs, as clipping to the linkers connecting either the targeting domain or the T-cell binding domain ultimately has a detrimental effect on the potency and efficacy of the drug. Clipping to additional sites, including the scFc, may affect pharmacodynamic / kinetic properties. For this reason, a panel of BiTEs was evaluated for protein clipping at TO, 2W, and 4W using reduced capillary electrophoresis (rCE). Results of this experiment are shown in Figure 11. For clarity, exemplary rCE traces of the Flt3-444 mutant at 40 °C and TO and 4W are shown, with the LMW peak and main peak regions demarcated. Although the Flt3 BiTE showed the highest level of clipping, the Flt-444 mutant performed better than the WT HLE-BiTE, with 10-15% fewer clipping events. It should also be noted that the "tight" Flt3-233 protein behaves like WT, while the "loose" Flt3-555 protein with the longest linker shows the greatest degree of clipping at 2W, and furthermore by 4W these clipped segments have been degraded. Surprisingly, for the "medium and loose" Msln and Dll3, i.e., 444 and 555 proteins, we observed a 5-10% reduction in clipping in samples stressed at 4W and 40°C. There appeared to be an effect on protein clipping depending on the linker length. This data suggests that in general, the 444 HHLL configuration appears to be well tolerated across the panel of HLE-BiTEs.

[0189] Frozen (-20℃) stability 100 μL aliquots of each 1 mg / ml protein sample were frozen in triplicate at -20°C. Protein samples were thawed (at room temperature) at t0, 2 weeks, 4 weeks, and 8 weeks. Upon thawing, samples were immediately subjected to size exclusion chromatography HPLC to quantify the percentage of the main peak. Comparable frozen stability to the WT bispecific construct was obtained for HHLL constructs of various linker lengths (e.g., 444 and 555 constructs).

[0190] Example 6 Freeze-thaw stability assay 100 μL of each 1 mg / ml protein sample was frozen in triplicate in 1.5 mL Eppendorf tubes. The protein was thawed by removing it from the -80°C freezer and bringing it to room temperature, then frozen again by returning the protein to the -80°C freezer. Samples were tested for 1, 5, and 10 freeze-thaw cycles. Results are shown in Figure 15. Comparable stability to the WT bispecific construct is obtained for HHLL constructs of various linker lengths (e.g., 444 and 555 constructs). Although the HMW of HHLL is high after one freeze-thaw, the HMW decreases uniformly across freeze-thaw cycles, indicating that the HHLL construct is compatible with freeze-thaw.

[0191] Example 7 Cell-based activity assays Target cell viability was measured by quantifying constitutively expressed firefly luciferase and was performed by the Steady-Glo Luciferase Assay System (Promega). Briefly, in this assay, HuT-78 cells, a human cutaneous T-cell lymphocyte cell line expressing CD3, were incubated with OVCAR-8-Luc cells, a human ovarian cancer cell line expressing mesothelin, engineered to constitutively express luciferase as a marker for cell number and viability. Msln BiTE protein was diluted in triplicate ranging from 0.01ng / mL to 5ng / mL and placed into 96-well, full area, flat bottom, tissue culture treated, sterile, white polystyrene plates (Costar, #3917). Cells were added to plates containing HutT-78 T cells and OVCAR-8-Luc Msln expressing cells at a ratio of 10:1, respectively, and incubated for a minimum of 24 hours before the addition of Steady-Glo reagent. Reconstituted Steady-Glo reagent was added (25 μL per well) according to Promega's protocol, and the assay plate was incubated at room temperature for 30 min. Luminescence was quantified by an EnVision multilabel reader (Perkin Elmer) equipped with an ultrasensitive luminescence detector. Data were normalized to 100% using Msln-WT as a reference / control.

[0192] This cell-based cytotoxicity assay was used to evaluate the activity of a panel of Flt3 and Msln HHLL BiTEs in T cell-mediated cell killing. The assay used human-derived T cells and human ovarian cancer cell lines expressing both Flt3 and Msln markers, and a luciferase reporter incubated with either Flt3 or Msln BiTE WT and HHLL molecules. For simplicity, potency data was normalized to the respective WT HLE-BiTE. All Flt3-HHLL BiTEs retained similar potency to the WT. An overall reduction in potency with Msln-HHLL BiTEs of approximately 75% was observed compared to the WT Msln HLE-BiTE. The results of this experiment are shown in Figure 13.

[0193] All documents cited herein are incorporated by reference in their entirety for all purposes.

[0194] The present invention is not limited in scope by the specific embodiments described herein, which are intended as single descriptions of individual inventive embodiments and functionally equivalent inventive methods and components. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to be included within the scope of the appended claims.

[0195] array Exemplary Linker Sequences GGGGS (SEQ ID NO: 1) GGGGSGGGGS (SEQ ID NO:2) GGGGSGGGGSGGGGS (SEQ ID NO: 3) GGGGSGGGGSGGGGSGGGGS (SEQ ID NO: 4) GGGGSGGGGSGGGGSGGGGSGGGGS (SEQ ID NO:5) GGGGQ (SEQ ID NO:6) GGGGQGGGGQ (SEQ ID NO: 7) GGGGQGGGGQGGGGQ (SEQ ID NO: 8) GGGGQGGGGQGGGGQGGGGQ (SEQ ID NO: 9) GGGGQGGGGQGGGGQGGGGQGGGGQ (SEQ ID NO: 10) GGGGSAAA (SEQ ID NO: 11) TVAAP (SEQ ID NO: 12) ASTKGP (SEQ ID NO: 13) AAA (SEQ ID NO: 14) GGNGT (SEQ ID NO: 15) YGNGT (SEQ ID NO: 16)

[0196] Please note that the following sequence includes an N-terminal signal peptide that is removed upon expression. The signal peptide sequence is: MDMRVPAQLLGLLLLWLRGARC (SEQ ID NO:52).

[0197] Target 1: dll3_332 (SEQ ID NO: 17) [ka]

[0198] Target 2: dll3_444 (SEQ ID NO: 18) [ka]

[0199] Target 3: dll3_555 (SEQ ID NO: 19) [ka]

[0200] Target 4: dll3_WT_intact_HLHL_scFCfromFlt3 (SEQ ID NO: 20) [ka]

[0201] Target 5: FLT3_ _AT_Kozak (WT) (SEQ ID NO: 21) [ka]

[0202] Target 6: FLT3_233 (SEQ ID NO: 22) [ka]

[0203] Target 7: FLT3_444 (SEQ ID NO: 23) [ka]

[0204] Target 8: FLT3_555 (SEQ ID NO: 24) [ka]

[0205] Target 9: MSLN_444_noscFC (SEQ ID NO: 25) [ka]

[0206] Target 10: MSLN_H1L1H2L2_no_scFC (WT) (SEQ ID NO: 26) [ka]

[0207] Target 11: MSLN_ _H1L2L1H2_noSCFC (SEQ ID NO: 27) [ka]

[0208] Target 12: MSLN_L1H2H1L2_noscFC (SEQ ID NO: 28) [ka]

[0209] Target 13: MSLN L1L2H1H2_noscFC (SEQ ID NO: 29) [ka]

[0210] Target 14: MSLN_L2H1H2L1_noscFC (SEQ ID NO: 30) [ka]

[0211] Target 15: MSLN_233 (SEQ ID NO: 31) [ka]

[0212] Target 16: MSLN 355 (SEQ ID NO: 32) [ka]

[0213] Target 17: MSLN_444 (SEQ ID NO: 33) [ka]

[0214] Target 18: MSLN_555 (SEQ ID NO: 34) [ka]

[0215] Target 19: MSLN_with scFC (WT) (SEQ ID NO: 35) [ka]

[0216] Fc region (36~39)

[0217] [Table 4]

[0218] SEQ ID NO: 40 Amino acid sequence of mature human CD3ε [ka]

[0219] SEQ ID NO: 41: Amino acid sequence of mature CD3ε from cynomolgus monkey [ka]

[0220] SEQ ID NO: 42 Amino acid sequence of the extracellular domain of human CD3ε [ka]

[0221] SEQ ID NO: 43 Amino acids 1-27 of human CD3ε qdgneemgg itqtpykvsi sgttvilt

[0222] DLL3 Heavy (SEQ ID NO:44) [ka]

[0223] DLL3 Light (SEQ ID NO: 45) EIVLTQSPGTLSLSPGERVTLSCRASQRVNNNYLAWYQQRPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYDRSPLTFGCGTKLEIK

[0224] FLT3 Heavy (SEQ ID NO:46) [ka]

[0225] FLT3 Light (SEQ ID NO: 47) DIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASTLQSGVPSRFSGSGSGTEFTLTISSLQPEDFATYYCLQHNSYPLTFGCGTKVEIK

[0226] MSLN Heavy (SEQ ID NO:48) [ka]

[0227] MSLN Light (SEQ ID NO: 49) DIQMTQSPSSVSASVGDRVTITCRASQGINTWLAWYQQKPGKAPKLLIYGASGLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQAKSFPRTFGQGTKVEIK

[0228] CD3 Heavy (SEQ ID NO:50) [ka]

[0229] CD3 Light (SEQ ID NO:51) QTVVTQEPSLTVSPGGTVTLTCGSSTGAVTSGNYPNWVQQKPGQAPRGLIGGTKFLAPGTPARFSGSLLGGKAALTLSGVQPEDEAEYYCVLWYSNRWVFGGGTKLTVL

Claims

1. A bispecific binding construct comprising a polypeptide chain comprising an amino acid sequence having the formula VH1-L1-VH2-L2-VL1-L3-VL2, wherein VH1 and VH2 are immunoglobulin heavy chain variable regions, VL1 and VL2 are immunoglobulin light chain variable regions, L1, L2 and L3 are linkers, L1 is at least 25 amino acids, L2 is at least 25 amino acids, and L3 is at least 25 amino acids, and wherein said bispecific binding construct is capable of binding to immune effector cells and target cells, the immune effector cell is a T cell that expresses an effector cell protein that is part of the human T cell receptor (TCR)-CD3 complex; The target cell is a cancer cell and the target is Msln or Dll3. Bispecific binding constructs.

2. 2. The bispecific binding construct of claim 1, further comprising a half-life extending moiety next to the VL2.

3. 3. The bispecific binding construct of claim 2, wherein the half-life extending moiety comprises an additional linker (L4) and a single chain immunoglobulin Fc region (scFc) derived from a human IgG1, IgG2, or IgG4 antibody.

4. 4. The bispecific binding construct of claim 3, wherein the scFc polypeptide chain comprises one or more modifications that inhibit Fcγ receptor (FcγR) binding and / or one or more modifications that extend half-life.

5. 2. The bispecific binding construct of claim 1, wherein all of said VH1, VH2, VL1, and VL2 have different sequences.

6. 2. The bispecific binding construct of claim 1, wherein the VH2 sequence comprises SEQ ID NO:50, the VL2 sequence comprises SEQ ID NO:51, the VH1 sequence comprises SEQ ID NO:44, the VL1 sequence comprises SEQ ID NO:45, or the VH1 sequence comprises SEQ ID NO:48 and the VL1 sequence comprises SEQ ID NO:

49.

7. 2. The bispecific binding construct of claim 1, wherein L1, L2 and L3 are of different lengths.

8. 2. The bispecific binding construct of claim 1, wherein L1, L2 and L3 are the same length.

9. 2. The bispecific binding construct of claim 1, wherein L1 and L2 are the same length.

10. 2. The bispecific binding construct of claim 1, wherein L1 and L3 are the same length.

11. 2. The bispecific binding construct of claim 1, wherein L2 and L3 are the same length.

12. 2. The bispecific binding construct of claim 1, wherein said bispecific binding construct exhibits improved stability compared to a bispecific binding construct having the formula VH1-L1-VL1-L2-VH2-L3-VL2.

13. 2. The bispecific binding construct of claim 1, wherein the bispecific antibody exhibits improved in vitro expression compared to a bispecific binding construct having the formula VH1-L1-VL1-L2-VH2-L3-VL2.

14. 2. The bispecific binding construct of claim 1 , wherein the effector cell protein is the CD3 epsilon chain.

15. A nucleic acid encoding a bispecific binding construct according to claims 1 to 14.

16. A vector comprising the nucleic acid of claim 15.

17. A host cell comprising the vector of claim 16.

18. 10. A method for producing the bispecific binding construct of claim 1, comprising: (1) culturing a host cell under conditions such that the bispecific binding construct is expressed; and (2) recovering the bispecific binding construct from the cell mass or from the culture supernatant, the host cell comprising one or more nucleic acids encoding the bispecific binding construct of any one of claims 1 to 9.

19. A pharmaceutical composition for treating a cancer patient comprising a therapeutically effective amount of a bispecific binding construct according to any one of claims 1 to 14.

20. A pharmaceutical composition comprising a bispecific binding construct according to any one of claims 1 to 14.

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