Macrolide derivatives, their production method and use

Abstract

To provide a novel macrolide derivative, and a preparation method thereof.SOLUTION: Provided are a macrolide derivative represented by formula (I), a preparation method thereof, and an application of the macrolide derivative as an inhibitor of one or more protein kinases of TRK, ALK and ROS1.SELECTED DRAWING: None

Description

Detailed Description of the Invention

[0001] [Technical field] The present invention relates to novel macrolide derivatives, processes for their preparation, pharmaceutical compositions containing said derivatives and their use as therapeutic agents, in particular as inhibitors of one or more of the protein kinases TRK, ALK and ROS1.

[0002] [Background technology] Tropomyosin-related receptor tyrosine kinases (TRKs) are high-affinity receptors for neurotrophins (NTs), the neurotrophic factor (NGF) family of proteins. The neurotrophic factor receptor tyrosine kinase genes NTRK1, NTRK2, and NTRK3 encode the TRKA, TRKB, and TRKC proteins, respectively. These TRKA, TRKB, and TRKC proteins are all tyrosine kinases and are collectively referred to as TRK family proteins. All TRK proteins have similar extracellular domain structures, but each has a different ligand: NGF binds to TRKA, brain-derived neurotrophic factor (BDNF) and neurotrophic factor 4 (NT-4) bind to TRKB, and neurotrophic factor 3 (NT-3) binds to TRKC.

[0003] Gene expression studies show that members of the TRK family are widely expressed in neural tissues and are involved in the maintenance, signal transduction and survival of neural cells. NTRK genes are mainly expressed in the nervous system, and also in embryonic development and adults. When activated by signal induction, TRK can undergo autophosphorylation, activating downstream signal pathways to carry out various physiological functions. The downstream signal molecules of TRK include SHC, FRS2, PLCγ, MAPK, PI3K, PKC, etc. Most of these signal molecules are closely related to functions including cellular energy exchange, survival and proliferation. When TRK is dysfunctional, the physiological functions of cells can become uncontrollable, and cells can even become cancer cells. Overexpression, activation, amplification and mutation of TRK proteins, as well as TRK gene fusion, are associated with many cancers. TRK gene fusion refers to the fusion of a member of the NTRK gene family (NTRK1, NTRK2, NTRK3) with another unrelated gene due to chromosomal mutation. TRK fusion proteins can become persistently active, triggering a permanent signal cascade reaction and driving the spread and growth of TRK fusion tumors. Cancers include neuroblastoma, ovarian cancer, breast cancer, prostate cancer, gastric cancer, gastrointestinal cancer, liver cancer, cholangiocarcinoma, pancreatic cancer, multiple myeloma, astrocytoma, medulloblastoma, glioma, melanoma, thyroid cancer, lung cancer, magnocellular neuroendocrine tumor, colorectal cancer, breast analog secretory carcinoma (MASC), sarcoma, head and neck tumor, renal cancer, etc.

[0004] TRK inhibitors are effective in preclinical pain models, and in particular the antagonistic NGF and TRKA antibody (RN-624) is effective in animal models of inflammatory and neuropathic pain, as well as in human clinical trials. In addition, activation of the BDNF / TRKB pathway may be used as a regulator of various types of pain, including inflammatory pain, neuropathic pain, and surgical pain. Because TRKA and RKB kinases may be used as mediators of NGF-driven biological responses, TRKA and / or other TRK kinase inhibitors may provide effective treatment for chronic pain. TRK inhibitors may effectively treat inflammatory pain, including but not limited to asthma, interstitial cystitis, ulcerative colitis, inflammatory bowel disease including Crohn's disease, eczema, psoriasis, and the like. The TRKA receptor is critical for the disease process of Trypanosoma cruzi parasitic infection in the human host, and TRKA inhibitors may be used for the treatment of Chagas disease and related protozoal infections. The TRK / neurotrophin pathway, which also refers to BDNF / TRKB, is also associated with the pathogenesis of neurodegenerative diseases, including multiple sclerosis, Parkinson's disease and Alzheimer's disease. TRK inhibitors can also be used to treat diseases associated with imbalances in bone remodeling regulations, such as osteoporosis, rheumatoid arthritis and bone metastasis.

[0005] Anaplastic lymphoma kinase (ALK) is a member of the insulin receptor superfamily of receptor tyrosine kinases and is closely associated with the oncogenesis of hematopoietic and non-hematopoietic tumors. The ALK gene is located on chromosome 2 and is expressed primarily in neurons, especially during development. The ALK gene is involved in a balanced chromosomal translocation of the nucleolar phosphoprotein (NPM) gene on chromosome 5 in a large subset of anaplastic large cell lymphomas (ALCLs). In ALK+ALCLs, as a result of the translocation, the ubiquitous promoter of NPM drives the ectopic expression of a fusion protein, in which NPM is partially dimerized and the ALK kinase domain undergoes autophosphorylation and becomes constitutively active. Full-length ALK receptor protein has been reported to be aberrantly expressed in neuroblastomas and glioblastomas. In addition, ALK fusion proteins have emerged in degenerative large cell lymphomas. Research on ALK fusion proteins has promoted the feasibility of novel therapeutic approaches for patients with ALK-positive malignancies. ROS1 belongs to the insulin receptor superfamily and is similar to other tyrosine kinase receptor molecules. ROS1 is responsible for transmitting growth signals from the cell's external environment into the cell nucleus. Genetic alterations of ROS1, such as gene rearrangements, mutations or copy number gains, produce oncogenes that can cause cancer. ROS1 is in the form of a fusion protein (six different partners of ROS1) in NSCLC patients and has been found to be present in approximately 2% of patients suffering from NSCLC (Bergethon et al., 2012; Davies et al., 2012). Two other ROS1 gene rearrangements have been detected in various other cancers. These cancers include glioblastoma multiforme, cholangiocarcinoma, ovarian cancer, gastric adenocarcinoma, colorectal cancer, inflammatory myofibroblastoma, angiosarcoma and epithelioid hemangioendothelioma. ROS1 gene rearrangements result in a fusion protein with a constitutively active kinase domain that activates downstream signaling pathways resulting in oncogenic properties in cells, including uncontrolled proliferation and resistance to cell death by prolonging tumor cell survival.

[0006] In November 2018, the US Food and Drug Administration approved the listing of the TRK inhibitor larotrectinib, a novel oral drug, for treating patients with abnormal TRK mutations. Previous studies have shown that the NTRK gene, which codes for TRK, can be abnormally fused with other genes, leading to the growth of cancer in many parts of the body, and that larotrectinib can selectively inhibit TRK. Meanwhile, LOXO-195 (Loxo Oncology There are TRK kinase inhibitors being tested, including riboflavin (TP Therapeutics Inc, Phase 2) and repotrectinib (TP Therapeutics Inc, Phase 2). Currently, a series of patent applications for TRK inhibitors have been published, including WO2015089139A1, WO2006082392A1, WO2007123269A1, etc. Although some progress has been made in the research and application of TRK inhibitors, there is still significant room for improvement. Therefore, there remains a need to continue the research and development of novel TRK inhibitors.

[0007] 〔overview〕 In view of the above technical problems, the present invention provides a novel macrolide derivative represented by general formula (I), which can significantly improve the activity of wild-type and mutant TRKA, TRKB and TRKC enzymes. Meanwhile, the compound of the present invention has good pharmacokinetic absorption, significantly increased Cmax, low clearance rate, significantly improved bioavailability and favorable pharmacokinetic properties.

[0008] Thus, in a first aspect, the present invention relates to a compound represented by general formula (I) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof,

[0009] [ka]

[0010] During the ceremony: Ring A is selected from bicyclic heteroaryls; Ring B is selected from a bicyclic aryl, a bicyclic heteroaryl or a bicyclic fused ring, wherein said bicyclic fused ring is preferably a fused ring of an aryl or heteroaryl with a monocyclic heterocyclyl or a monocyclic cycloalkyl; L1 is -(CR a R b ) m -, where -(CR a R b )- is optionally -N(R c )-, -O- or -S(O) r - has been further replaced by; L2 is -(CR d R e ) n -, where -(CR d R e )- is optionally -N(R f )-, -O- or -S(O) r - has been further replaced by; R a , R b , R d and R e are the same or different and each independently represent a hydrogen atom, deuterium, halogen, hydroxyl, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, heteroaryl, or -NR 5 R 6 wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally selected from hydroxyl, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6and is further substituted by one or more substituents selected from; Alternatively, R a and R b together with the same carbon atom to which they are attached form a C3-C8 cycloalkyl or a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) r wherein the C3-C8 cycloalkyl or 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, R a Any two of these, together with the different carbon atoms to which they are each bonded, form a C3-C8 cycloalkyl or a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) r wherein the C3-C8 cycloalkyl or 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, R d and R e together with the same carbon atom to which they are attached form a C3-C8 cycloalkyl or a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) r wherein the C3-C8 cycloalkyl or 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, R d Any two of these, together with the different carbon atoms to which they are each bonded, form a C3-C8 cycloalkyl or a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) r wherein the C3-C8 cycloalkyl or 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; R c and Rf are each the same or different and each independently selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; R c and R f is preferably selected from a hydrogen atom; Alternatively, one -(CR a R b )--N(R c )-, R a or R b and R c together with the carbon and nitrogen atoms to which they are respectively bonded form a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) r wherein the 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O, —C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, one -(CR d R e )--N(R f )-, R d or R e and R f together with the carbon and nitrogen atoms to which they are respectively bonded form a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) rwherein the 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O, —C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; R 1 and R 2 are the same or different and each independently represent a hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O, —C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6and preferably, R 1 and R 2 are each independently selected from a hydrogen atom, halogen, amino, cyano, alkyl, alkoxy, haloalkyl, haloalkoxy, hydroxyalkyl, or alkoxyalkyl; more preferably, R 1 and R 2 are each independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl; R 3 is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; R 4 , R 5 and R 6 are each independently selected from a hydrogen atom, hydroxy, halogen, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl, wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally selected from hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 7 , -C(O)OR 7 , -OC(O)R 7 , -NR 8 R 9 , -C(O)NR 8 R 9 , -SO2NR 8 R 9 or -NR 8 C(O)R 9 and is further substituted by one or more substituents selected from; Alternatively, R 5 and R 6 together with the atom to which they are attached form a 4-8 membered heterocyclyl, wherein the 4-8 membered heterocyclyl is selected from the group consisting of one or more N, O, or S(O)r wherein the 4-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 7 , -C(O)OR 7 , -OC(O)R 7 , -NR 8 R 9 , -C(O)NR 8 R 9 , -SO2NR 8 R 9 or -NR 8 C(O)R 9 and is further substituted by one or more substituents selected from; R 7 , R 8 and R 9 are each independently selected from a hydrogen atom, an alkyl, a cycloalkyl, a heterocyclyl, an aryl, or a heteroaryl, wherein said alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl is optionally further substituted by one or more substituents selected from hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, carboxyl, or carboxylate; m and n are the same or different and are each independently selected from 1, 2, 3, or 4; p and q are the same or different and are each independently selected from 0, 1, 2, 3, 4 or 5; and r is selected from 0, 1 or 2; The present invention provides a compound, or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof.

[0011] In some preferred embodiments of the present invention, the compound represented by general formula (I), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, is a compound represented by general formula (II), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof:

[0012] [ka]

[0013] During the ceremony: Ring B is selected from:

[0014] [ka]

[0015] Ring C is selected from monocyclic cycloalkyl, monocyclic heterocyclyl, monocyclic aryl, or monocyclic heteroaryl, where Ring C is bonded to L2; X is -N(R c )-, -O- or -S(O) r -; preferably -NH-; Z 1 , Z 2 , and Z 4 ~Z 6 are the same or different and each independently represents N, NH, C(=O) or C(R 1 ) are selected from; Z 3 and Z 7 are the same or different and each is independently selected from N or C; Z 1 ~Z 7 at least one of is not selected from N or NH; X 1 ~X 4 are the same or different and each independently represents a bond, N, NH, C(=O) or C(R 2 ) are selected from;X 1 ~X 4 At least one of X is not selected from N or NH; 1 ~X 4 at most one of is selected from a bond; R Ais selected from a hydrogen atom or an alkyl, wherein said alkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; R A is preferably methyl; R c is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; Alternatively, X is -N(R c )-, R A and R c together with the carbon atom and nitrogen atom to which they are respectively bonded, form a 4-8 membered heterocyclyl, said 4-8 membered heterocyclyl being one or more of N, O or S(O) r wherein the 4-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and R 1 ~R 6 , L2, q and r are as defined in general formula (I).

[0016] In some preferred embodiments of the present invention, the compound represented by general formula (II), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, is a compound represented by general formula (III), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof,

[0017] [ka]

[0018] In the formula: Ring B, X, L2, R 1 ~R 3 , R A and q are as defined in general formula (II).

[0019] In some preferred embodiments of the present invention, in the compound represented by general formula (I), (II) or (III), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, ring B is selected from 8- to 10-membered bicyclic fused rings, preferably selected from the following:

[0020] [ka]

[0021] In some preferred embodiments of the present invention, in the compounds represented by general formula (I), (II) or (III), or stereoisomers or tautomers thereof, or pharma- ceutically acceptable salts thereof, ring B is selected from 8-10 membered bicyclic heteroaryls, preferably selected from the following:

[0022] [ka]

[0023] In some preferred embodiments of the present invention, the compound represented by general formula (III), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, is a compound represented by general formula (IV) or (V), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof:

[0024] [ka]

[0025] During the ceremony: G 1 and G 2 are the same or different and each independently represents a bond, -N(R g )-, -(CR h R i )- or -O-; R g is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy or alkoxy; R h and R i are the same or different and are each independently selected from a hydrogen atom, deuterium, halogen, hydroxy, alkoxy, alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl, wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl is optionally selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, R h and R i together with the carbon atom to which they are attached form a C3-C8 cycloalkyl or a 3-8 membered heterocyclyl, wherein the 3-8 membered heterocyclyl is selected from the group consisting of one or more N, O or S(O) rwherein the C3-C8 cycloalkyl or 3-8 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; R 1 and R 2 are the same or different and each independently represent a hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally further substituted by one or more substituents selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl or heterocyclyl; preferably, R 1 and R 2 are each independently selected from a hydrogen atom, halogen, amino, cyano, alkyl, alkoxy, haloalkyl, haloalkoxy, hydroxyalkyl, or alkoxyalkyl; more preferably, R 1 and R 2are each independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl; R B is selected from a hydrogen atom, an alkyl or an alkoxy, wherein said alkyl or alkoxy is hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and preferably, R B is selected from a hydrogen atom or methyl, wherein said methyl is optionally selected from halogen, hydroxy, cycloalkyl, alkoxy or -NR 5 R 6 and is further substituted by one or more substituents selected from; R D and R E are the same or different and each independently represent a hydrogen atom, a deuterium atom, a halogen atom, a hydroxyl group, an alkoxy group, an alkyl group, an alkenyl group, an alkynyl group, a cycloalkyl group, a heterocyclyl group, an aryl group, a heteroaryl group, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6wherein said alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and is further substituted by one or more substituents selected from; Alternatively, R D and R E together with the carbon atom to which they are attached form a C3-C6 cycloalkyl or a 3- to 6-membered heterocyclyl, wherein the 3- to 6-membered heterocyclyl is selected from the group consisting of one or more N, O, or S(O) r wherein the C3-C6 cycloalkyl or 3-6 membered heterocyclyl is optionally selected from the group consisting of hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 4 , -C(O)OR 4 , -OC(O)R 4 , -NR 5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 and L2, r and R 3 ~R 6 is as defined in general formula (III).

[0026] In some preferred embodiments of the present invention, the compound represented by general formula (III), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, is a compound represented by general formula (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof,

[0027] [ka]

[0028] In the formula: L2, G 1 , G 2 , R 1 ~R 3 , R B , R D and R E is as defined in general formula (IV) or (V).

[0029] In some preferred embodiments of the present invention, the compounds represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, are represented by the formula: L2 is -(CR d R e ) n - Selected from; R d is selected from a hydrogen atom; R e is selected from a hydrogen atom, an alkyl or an alkoxy, preferably a hydrogen atom or a methyl, wherein said alkyl or alkoxy is optionally further substituted by one or more halogens; and n is 1, 2 or 3.

[0030] Exemplary compounds of the present invention, or stereoisomers, tautomers, or pharma- ceutically acceptable salts thereof, include, but are not limited to, the following:

[0031] [Table 1-1]

[0032] [Table 1-2]

[0033] [Table 1-3]

[0034] [Table 1-4]

[0035] [Table 1-5]

[0036] In another aspect, the present invention provides a method for preparing a compound represented by general formula (I), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, comprising the steps of: The method includes:

[0037] [ka]

[0038] subjecting a compound represented by general formula (IA) to a condensation reaction under basic conditions to obtain a compound represented by general formula (I); In the formula: Ring A, Ring B, R 1 ~R 3 , L1, L2, p and q are as defined in general formula (I); A method is provided.

[0039] In another aspect, the present invention provides a method for preparing a compound represented by general formula (I), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, comprising the steps of: The method includes:

[0040] [ka]

[0041] subjecting the compound represented by general formula (If) to a condensation reaction under basic conditions to obtain a compound represented by general formula (I); During the ceremony: R j is selected from alkyl; and Ring A, Ring B, R 1 ~R 3 , L1, L2, p and q are as defined in general formula (I); A method is provided.

[0042] In another aspect, the present invention relates to a compound represented by the general formula (IA) or a stereoisomer or tautomer thereof,

[0043] [ka]

[0044] In the formula: Ring A, Ring B, R 1 ~R 3 , L1, L2, p and q are as defined in general formula (I); A compound, or a stereoisomer or tautomer thereof, is provided.

[0045] Exemplary compounds represented by general formula (IA), or stereoisomers, tautomers, or pharma- ceutically acceptable salts thereof, include, but are not limited to, the following:

[0046] [Table 2-1]

[0047] [Table 2-2]

[0048] [Table 2-3]

[0049] In another aspect, the present invention provides a method for preparing a compound of general formula (IA) or a stereoisomer or tautomer thereof, comprising the steps of: The method includes:

[0050] [ka]

[0051] reacting a compound represented by general formula (Ic) in the presence of di-tert-butyl dicarbonate, hydrogen and a catalyst to obtain a compound represented by general formula (Id); subjecting said compound of general formula (Id) to hydrolysis under basic conditions to obtain a compound of general formula (Ie); and removing the protecting group PG from the compound represented by general formula (Ie) to obtain a compound represented by general formula (IA); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; PG is an amino protecting group, preferably tert-butoxycarbonyl; and Ring A, Ring B, R 1 ~R 2 , L1, L2, p and q are as defined in general formula (I); A method is provided.

[0052] The present invention further provides a pharmaceutical composition comprising an effective amount of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, and a pharma- ceutically acceptable carrier, excipient, or combination thereof.

[0053] The present invention further provides a method for inhibiting the activity of one or more of the protein kinases TRK, ALK and ROS1, comprising contacting a TRK receptor with a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof.

[0054] The present invention further provides the use of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof, in the manufacture of a medicament for treating a disease mediated by one or more of the protein kinases TRK, ALK and ROS1, wherein the disease mediated by one or more of the protein kinases TRK, ALK and ROS1 is preferably pain, cancer, inflammation, a neurodegenerative disease or a trypanosoma infection, and wherein the cancer is preferably neurocytoma, ovarian cancer, breast cancer, prostate cancer, gastric cancer, gastrointestinal cancer, liver cancer, bile duct cancer, pancreatic cancer, multiple myeloma, astrocytoma, medulloblastoma, glioma, melanoma, thyroid cancer, lung cancer, magnocellular neuroendocrine tumor, colorectal cancer, breast analog secretory breast cancer, sarcoma, head and neck tumor and renal cancer.

[0055] The present invention further provides the use of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof, in the manufacture of an inhibitor of one or more of the protein kinases TRK, ALK and ROS1.

[0056] The present invention further provides the use of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof, in the manufacture of a medicament for treating pain, cancer, inflammation, neurodegenerative disease or trypanosoma infection, wherein the cancer is preferably neurocytoma, ovarian cancer, breast cancer, prostate cancer, gastric cancer, gastrointestinal cancer, liver cancer, bile duct cancer, pancreatic cancer, multiple myeloma, astrocytoma, medulloblastoma, glioma, melanoma, thyroid cancer, lung cancer, magnocellular neuroendocrine tumor, colorectal cancer, breast analog secretory breast cancer, sarcoma, head and neck tumor and renal cancer.

[0057] The present invention further provides a method for treating pain, inflammation, symptoms of a neurodegenerative disease or a trypanosomal infection, comprising administering to a patient in need thereof a therapeutically effective amount of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof.

[0058] The present invention further provides a method for treating cancer, comprising administering a therapeutically effective amount of a compound represented by general formula (I), (II), (III), (IV), (V), (VI), (VII), (VIII) or (IX) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof, to a patient in need thereof, wherein the cancer is preferably neurocytoma, ovarian cancer, breast cancer, prostate cancer, gastric cancer, gastrointestinal cancer, liver cancer, bile duct cancer, pancreatic cancer, multiple myeloma, astrocytoma, medulloblastoma, glioma, melanoma, thyroid cancer, lung cancer, magnocellular neuroendocrine tumor, colorectal cancer, breast analog secretory breast cancer, sarcoma, head and neck tumor and renal cancer.

[0059] Detailed Description of the Invention Unless stated to the contrary, several terms used in the present specification and claims are defined as follows.

[0060] "Bond" means that the indicated substituent is not present and both ends of the substituent are directly connected to form a bond.

[0061] "Alkyl" when considered as a group or part of a group means C1-C 20 It is meant to include linear or branched aliphatic hydrocarbon groups. Alkyl is preferably C1-C 10 It is preferably an alkyl group, more preferably a C1-C6 alkyl group. Examples of alkyl groups include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, tert-butyl, sec-butyl, n-pentyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 2,2-dimethylpropyl, 1-ethylpropyl, 2-methylbutyl, 3-methylbutyl, n-hexyl, 1-ethyl-2-methylpropyl, 1,1,2-trimethylpropyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1,3-dimethylbutyl, 2-ethylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 2,3-dimethylbutyl, and the like. The alkyl group may be substituted or unsubstituted.

[0062] "Alkenyl" refers to an alkyl as defined above, consisting of at least two carbon atoms and at least one carbon-carbon double bond. Representative examples of alkenyl include, but are not limited to, ethenyl, 1-propenyl, 2-propenyl, 1-, 2- or 3-butenyl, and the like. Alkenyl may be substituted or unsubstituted.

[0063] "Alkynyl" refers to an aliphatic hydrocarbon group having one carbon-carbon triple bond. Alkynyl may be linear or branched. Alkynyl is preferably C2-C6 10 Alkynyl, more preferably C2-C6 alkynyl, most preferably C2-C4 alkynyl. Examples of alkynyl groups include, but are not limited to, ethynyl, 1-propynyl, 2-propynyl, 1-, 2- or 3-butynyl, etc. Alkynyl may be substituted or unsubstituted.

[0064] "Cycloalkyl" refers to saturated or partially saturated monocyclic, fused, bridged and spirocyclic carbocycles. Cycloalkyl is preferably C3-C 12 Cycloalkyl is preferably C3-C8 cycloalkyl, and most preferably C3-C6 cycloalkyl. Examples of monocyclic cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cyclohexadienyl, cycloheptyl, cycloheptolienyl, cyclooctyl, and the like, with cyclopropyl and cyclohexenyl being preferred. Cycloalkyl may be optionally substituted or unsubstituted.

[0065] "Spirocycloalkyl" refers to a 5-18 membered polycyclic group having two or more ring structures, with the single rings sharing one carbon atom (called a spiro atom) with each other. The rings contain one or more double bonds, but none of the rings have a fully conjugated pi-electron aromatic system. Spirocycloalkyls are preferably 6-14 membered, more preferably 7-10 membered. Depending on the number of spiro atoms shared between the rings, spirocycloalkyls may be classified as monospiro, dispiro or multispirocycloalkyls, preferably monospiro and dispirocycloalkyls, preferably 4-membered / 5-membered, 4-membered / 6-membered, 5-membered / 5-membered, or 5-membered / 6-membered. Non-limiting examples of "spirocycloalkyls" include, but are not limited to, spiro[4.5]decyl, spiro[4.4]nonyl, spiro[3.5]nonyl, and spiro[2.4]heptyl.

[0066] "Fused cycloalkyl" refers to a 5-18 membered all-carbon polycyclic group having two or more ring structures sharing a pair of carbon atoms, where one or more rings may contain one or more double bonds, but none of the rings has a fully conjugated π-electron aromatic system. Fused cycloalkyls are preferably 6-12 membered, more preferably 7-10 membered. Depending on the number of constituent rings, fused cycloalkyls may be classified as bicyclic, tricyclic, tetracyclic or polycyclic fused cycloalkyls, preferably bicyclic or tricyclic, more preferably 5-membered / 5-membered or 5-membered / 6-membered bicycloalkyls. Non-limiting examples of "fused cycloalkyls" include, but are not limited to, bicyclo[3.1.0]hexyl, bicyclo[3.2.0]hept-1-enyl, bicyclo[3.2.0]heptyl, decalinyl or tetradecahydrophenanthrenyl.

[0067] "Bridged cycloalkyl" refers to a 5-18 membered all-carbon polycyclic group having two or more ring structures sharing two carbon atoms that are not directly bonded to each other, where one or more rings may contain one or more double bonds, but none of the rings has a fully conjugated π-electron aromatic system. The bridged cycloalkyl is preferably 6-12 membered, more preferably 7-10 membered. The bridged cycloalkyl is preferably 6-14 membered, more preferably 7-10 membered. Depending on the number of constituent rings, the bridged cycloalkyl may be classified as a bicyclic, tricyclic, tetracyclic or polycyclic bridged cycloalkyl, preferably bicyclic, tricyclic or tetracyclic, more preferably bicyclic or tricyclic. Non-limiting examples of "bridged cycloalkyl" include, but are not limited to, (1s,4s)-bicyclo[2.2.1]heptyl, bicyclo[3.2.1]octyl, (1s,5s)-bicyclo[3.3.1]nonyl, bicyclo[2.2.2]octyl, and (1r,5r)-bicyclo[3.3.2]decyl.

[0068] The terms "heterocyclyl", "heterocycle" or "heterocyclic" are used interchangeably in this application. All refer to non-aromatic heterocyclyls in which one or more ring-forming atoms are heteroatoms such as oxygen, nitrogen, or sulfur atoms, and include monocyclic, fused, bridged, and spiro rings. Non-aromatic heterocyclyls preferably have 5-7 membered monocyclic rings or 7-10 membered bicyclic or tricyclic rings, and may contain 1, 2, or 3 atoms selected from nitrogen, oxygen, and / or sulfur. Examples of "heterocyclyl" include, but are not limited to, morpholinyl, oxetanyl, thiomorpholinyl, tetrahydropyranyl, 1,1-dioxo-thiomorpholinyl, piperidinyl, 2-oxo-piperidinyl, pyrrolidinyl, 2-oxo-pyrrolidinyl, piperazin-2-one, 8-oxa-3-aza-bicyclo[3.2.1]octyl, and piperazinyl. Heterocyclyl may be substituted or unsubstituted.

[0069] "Spiroheterocyclyl" refers to a 5- to 18-membered polycyclic group having two or more ring structures, where the single rings share one atom with each other. The rings contain one or more double bonds, but none of the rings has a completely conjugated pi-electron aromatic system, where one or more of the ring atoms is nitrogen, oxygen, or S(O) n (n is selected from 0, 1 or 2) heteroatoms, the remaining ring atoms being carbon. Spiroheterocyclyls are preferably 6-14 membered, more preferably 7-10 membered. Depending on the number of spiro atoms shared between the rings, spiroheterocyclyls may be classified as monospiroheterocyclyl, bispyroheterocyclyl or multispiroheterocyclyl, preferably monospiroheterocyclyl and bispyroheterocyclyl. Spiroheterocyclyls are more preferably 4-membered / 4-membered, 4-membered / 5-membered, 4-membered / 6-membered, 5-membered / 5-membered, or 5-membered / 6-membered monospiroheterocyclyl. Non-limiting examples of "spiroheterocyclyl" include, but are not limited to, 1,7-dioxaspiro[4.5]decyl, 2-oxa-7-azaspiro[4.4]nonyl, 7-oxaspiro[3.5]nonyl and 5-oxaspiro[2.4]heptyl.

[0070] "Fused heterocyclyl" refers to an all-carbon polycyclic group having two or more ring structures sharing a pair of atoms, where one or more of the rings may contain one or more double bonds, but none of the rings has a completely conjugated pi-electron aromatic system, where one or more of the ring atoms is nitrogen, oxygen, or S(O) n(n is selected from 0, 1 or 2) heteroatoms, the remaining ring atoms being carbon. The fused heterocyclyl is preferably 6-14 membered, more preferably 7-10 membered. Depending on the number of constituent rings, the fused heterocyclyl may be classified as bicyclic, tricyclic, tetracyclic or polycyclic fused heterocyclyl, preferably bicyclic or tricyclic, more preferably 5-membered / 5-membered or 5-membered / 6-membered bicyclic fused heterocyclyl. Non-limiting examples of "fused heterocyclyl" include, but are not limited to, octahydropyrrolo[3,4-c]pyrrolyl, octahydro-1H-isoindolyl, 3-azabicyclo[3.1.0]hexyl, and octahydrobenzo[b][1,4]dioxine.

[0071] "Bridged heterocyclyl" refers to a 5-14 or 5-18 membered polycyclic group having two or more ring structures that share two atoms that are not directly bonded to each other. One or more of the rings may contain one or more double bonds, but none of the rings has a completely conjugated pi-electron aromatic system, where one or more of the ring atoms is nitrogen, oxygen, or S(O) n (n is selected from 0, 1 or 2) heteroatoms, the remaining ring atoms being carbon. Bridged heterocyclyls are preferably 6-14 membered, more preferably 7-10 membered. Depending on the number of constituent rings, bridged heterocyclyls may be classified as bicyclic, tricyclic, tetracyclic or polycyclic bridged heterocyclyls, preferably bicyclic, tricyclic or tetracyclic, more preferably bicyclic or tricyclic. Non-limiting examples of "bridged heterocyclyls" include, but are not limited to, 2-azabicyclo[2.2.1]heptyl, 2-azabicyclo[2.2.2]octyl and 2-azabicyclo[3.3.2]decyl.

[0072] "Aryl" refers to a carbocyclic aromatic system containing one or two rings, where the rings may be joined together in a fused fashion. The term "aryl" includes monocyclic or bicyclic aryls such as the aromatic groups phenyl, naphthyl, and tetrahydronaphthyl. Preferably, aryl is a C6-C 10More preferably, aryl is phenyl and naphthyl, and most preferably, aryl is naphthyl. Aryl may be substituted or unsubstituted.

[0073] "Heteroaryl" refers to a 5-6 membered monocyclic ring or an 8-10 membered bicyclic ring, optionally containing 1-4 atoms selected from nitrogen, oxygen and / or sulfur. Heteroaryl is preferably a bicyclic heteroaryl. Examples of "heteroaryl" include, but are not limited to, furanyl, pyridinyl, 2-oxo-1,2-dihydropyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, thienyl, isoxazolyl, oxazolyl, oxadiazolyl, imidazolyl, pyrrolyl, pyrazolyl, triazolyl, tetrazolyl, thiazolyl, isothiazolyl, 1,2,3-thiadiazolyl, benzodioxolyl, benzothienyl, benzimidazolyl, indolyl, isoindolyl, 1,3-dioxo-isoindolyl, quinolinyl, indazolyl, benzisothiazolyl, benzoxazolyl, and benzisoxazolyl.

[0074] [ka]

[0075] The heteroaryl may be substituted or unsubstituted.

[0076] "Fused ring" refers to a polycyclic group having two or more ring structures that share a pair of atoms with each other. One or more of the rings may contain one or more double bonds, but at least one ring does not have a fully conjugated pi-electron aromatic system, while at least one ring does have a fully conjugated pi-electron aromatic system, where zero, one or more of the ring atoms are nitrogen, oxygen or S(O) n(n is selected from 0, 1 or 2) heteroatoms, with the remaining ring atoms being carbon. The fused ring is preferably a bicyclic or tricyclic fused ring, where the bicyclic fused ring is preferably a fused ring of an aryl or heteroaryl with a monocyclic heterocyclyl or a monocyclic cycloalkyl. The fused ring is preferably 7-14 members, more preferably 8-10 members. Examples of "fused rings" include, but are not limited to, the following:

[0077] [ka]

[0078] "Alkoxy" refers to the group (alkyl-O-). Here, for the definition of alkyl, please refer to the relevant definition in this specification. C1-C6 alkoxy is preferred. Examples of alkoxy include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, isobutoxy, tert-butoxy, etc.

[0079] "Haloalkyl" refers to an alkyl substituted by a halogen, where definitions of halogen and alkyl are referred to herein in the relevant definitions.

[0080] "Haloalkoxy" refers to an alkoxy substituted by halogen, where the definitions of halogen and alkoxy are as defined herein.

[0081] "Hydroxyalkyl" refers to alkyl substituted by hydroxy, where for a definition of alkyl, see relevant definition herein.

[0082] "Alkoxyalkyl" refers to an alkyl substituted by an alkoxy, where the definitions of alkyl and alkoxy are referred to herein in the relevant definitions.

[0083] "Hydroxy" refers to the group --OH.

[0084] "Halogen" refers to fluorine, chlorine, bromine and iodine.

[0085] "Amino" refers to -NH2.

[0086] "Cyano" refers to -CN.

[0087] "Nitro" refers to -NO2.

[0088] "Benzyl" refers to -CH2-phenyl.

[0089] "Carboxyl" refers to -C(O)OH.

[0090] "Carboxylate" refers to -C(O)O-alkyl or -C(O)O-cycloalkyl, where alkyl and cycloalkyl are defined above.

[0091] "DMSO" refers to dimethylsulfoxide.

[0092] "BOC" refers to tert-butoxycarbonyl.

[0093] "Ts" refers to p-toluenesulfonyl.

[0094] "Leaving group" is an atom or functional group that separates from a larger molecule in a chemical reaction, a term used in nucleophilic substitution and elimination reactions. In a nucleophilic substitution reaction, the reactant that is attacked by the nucleophile is called the substrate, while the atom or group of atoms that has an electron pair and separates in the substrate molecule is called the leaving group. A group that readily accepts electrons and has a strong ability to retain a negative charge is a good leaving group. The smaller the pKa of the conjugate acid of the leaving group, the easier it is for the leaving group to separate from the other molecule. The reason is that the smaller the pKa of the conjugate acid of the leaving group, the more likely the corresponding leaving group is to exist in the form of anion (or an electrically neutral leaving group) without the need to bond with other atoms. Common leaving groups include, but are not limited to, halogens, -OTs, or -OH.

[0095] "Substituted" means that one or more hydrogen atoms in the group, preferably at most 5, more preferably 1 to 3 hydrogen atoms, are independently replaced by the corresponding number of substituents. Naturally, the substituents are only present in their possible chemical positions, and the skilled artisan can determine (through experiment or theory) possible or impossible substitutions without much effort. For example, amino or hydroxyl with free hydrogen may be unstable when combined with a carbon atom with an unsaturated (e.g., olefinic) bond.

[0096] As used herein, unless otherwise stated, "substituted" or "substituted" means that a group may be substituted with one or more groups selected from the following: alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylamino, halogen, mercapto, hydroxy, nitro, cyano, cycloalkyl, heterocyclic, aryl, heteroaryl, cycloalkoxy, heterocyclic alkoxy, cycloalkylthio, heterocycloalkylthio, amino, haloalkyl, hydroxyalkyl, carboxyl, carboxylate, =O, -C(O)R. 4 , -C(O)OR 4 , -OC(O)R 4 , -NR5 R 6 , -C(O)NR 5 R 6 , -SO2NR 5 R 6 or -NR 5 C(O)R 6 .

[0097] R 4 , R 5 and R 6 are each independently selected from a hydrogen atom, hydroxy, halogen, alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl, where the alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, or heteroaryl is optionally selected from hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, =O, -C(O)R 7 , -C(O)OR 7 , -OC(O)R 7 , -NR 8 R 9 , -C(O)NR 8 R 9 , -SO2NR 8 R 9 or -NR 8 C(O)R 9 is further substituted by one or more substituents selected from:

[0098] Alternatively, R 5 and R 6 together with the atom to which they are attached form a 4-8 membered heterocyclyl, wherein the 4-8 membered heterocyclyl is selected from the group consisting of one or more N, O, or S(O) r wherein the 4-8 membered heterocyclyl is optionally substituted with hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O, —C(O)R 7 , -C(O)OR 7 , -OC(O)R 7 , -NR 8 R 9 , -C(O)NR 8 R9 , -SO2NR 8 R 9 or -NR 8 C(O)R 9 is further substituted by one or more substituents selected from:

[0099] R 7 , R 8 and R 9 are each independently selected from a hydrogen atom, an alkyl, a cycloalkyl, a heterocyclyl, an aryl, or a heteroaryl, where the alkyl, cycloalkyl, heterocyclyl, aryl, or heteroaryl is optionally further substituted by one or more substituents selected from hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, carboxyl, or carboxylate.

[0100] The compounds of the present invention may contain asymmetric or chiral centers and therefore may exist in various stereoisomeric forms. All stereoisomeric forms of the compounds of the present invention, including, but not limited to, diastereomers, enantiomers, and atropisomers, as well as geometric (conformational) isomers, and mixtures thereof, such as racemic mixtures, are intended to be within the scope of the present invention.

[0101] Unless otherwise specified, structures depicted in the present invention also include all isomers of the structures (e.g., diastereomers, enantiomers and antimetamers, as well as geometric (conformational) isomeric forms; e.g., R and S configurations of each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers). Thus, single stereoisomers as well as enantiomeric, diastereomeric, and geometric (conformational) isomeric mixtures of the compounds of the present invention are all within the scope of the present invention.

[0102] "Pharmaceutically acceptable salt" refers to any salt of the above-mentioned compound that can maintain its original biological activity and is suitable for medical use. The pharmaceutically acceptable salt of the compound represented by formula (I) may be a metal salt, an amine salt formed with a suitable acid.

[0103] A "pharmaceutical composition" refers to a mixture containing one or more compounds described herein or physiologically acceptable salts or prodrugs thereof, and other chemical components, as well as other components such as physiologically acceptable carriers and excipients. The purpose of a pharmaceutical composition is to facilitate administration to an organism and facilitate absorption of the active ingredients to exert a biological activity.

[0104] [Method of synthesizing the compound of the present invention] In order to achieve the objectives of the present invention, the following technical solutions are adopted by the present invention.

[0105] [Solution 1] The method for producing a compound represented by general formula (I) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0106] [ka]

[0107] A step of reacting a compound represented by general formula (Ia) with a compound represented by general formula (Ib) under basic conditions to obtain a compound represented by general formula (Ic); a step of reacting a compound represented by general formula (Ic) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (Id); a step of subjecting a compound represented by general formula (Id) to hydrolysis under basic conditions to obtain a compound represented by general formula (Ie); a step of further removing a protecting group PG from the compound represented by general formula (Ie) to obtain a compound represented by general formula (IA); and a step of subjecting a compound represented by general formula (IA) to a condensation reaction in the presence of a condensation reagent to obtain a compound represented by general formula (I); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; R k is a leaving group, preferably a halogen; PG is an amino protecting group, preferably tert-butoxycarbonyl; and Ring A, Ring B, R 1 ~R 2 , L1, L2, p and q are as defined in general formula (I).

[0108] [Solution 2] The method for producing a compound represented by general formula (I) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0109] [ka]

[0110] a step of reacting a compound represented by general formula (Ic) with triphenylphosphine and water to obtain a compound represented by general formula (If); and a step of subjecting the compound represented by general formula (If) to a condensation reaction under basic conditions to obtain a compound represented by general formula (I); R 3 is selected from a hydrogen atom; R j is selected from alkyl; and Ring A, Ring B, R 1 ~R 2 , L1, L2, p and q are as defined in general formula (I).

[0111] [Solution 3] The method for producing a compound represented by general formula (II) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0112] [ka]

[0113] A step of reacting a compound represented by general formula (IIa) with a compound represented by general formula (IIc) under basic conditions to obtain a compound represented by general formula (IIc); a step of reacting a compound represented by general formula (IIc) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (IId); a step of subjecting a compound represented by general formula (IId) to hydrolysis under basic conditions to obtain a compound represented by general formula (IIe); a step of further removing a protecting group PG from the compound represented by general formula (IIe) to obtain a compound represented by general formula (IIA); and a step of subjecting a compound represented by general formula (IIA) to a condensation reaction in the presence of a condensation reagent to obtain a compound represented by general formula (II); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; R k is a leaving group, preferably a halogen; PG is an amino protecting group, preferably tert-butoxycarbonyl; and Ring B, R 1 ~R 2 , R A , Z 1 ~Z 7 , X, L2, p and q are as defined in general formula (II).

[0114] [Solution 4] The method for producing a compound represented by general formula (II) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0115] [ka]

[0116] a step of reacting a compound represented by general formula (IIc) with triphenylphosphine and water to obtain a compound represented by general formula (IIf); and a step of subjecting the compound represented by general formula (IIf) to a condensation reaction under basic conditions to obtain a compound represented by general formula (II); R 3 is selected from a hydrogen atom; R j is selected from alkyl; and Ring B, R 1 ~R 2 , R A , Z 1 ~Z 7 , X, L2, p and q are as defined in general formula (II).

[0117] [Solution 5] The method for producing a compound represented by general formula (III) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0118] [ka]

[0119] A step of reacting a compound represented by general formula (IIIa) with a compound represented by general formula (IIIb) under basic conditions to obtain a compound represented by general formula (IIIc); a step of reacting a compound represented by general formula (IIIc) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (IIId); a step of subjecting a compound represented by general formula (IIId) to hydrolysis under basic conditions to obtain a compound represented by general formula (IIIe); a step of further removing a protecting group PG from the compound represented by general formula (IIIe) to obtain a compound represented by general formula (IIIA); and a step of subjecting a compound represented by general formula (IIIA) to a condensation reaction in the presence of a condensation reagent to obtain a compound represented by general formula (III); During the ceremony: R 3 is selected from a hydrogen atom; R jis selected from alkyl; R k is a leaving group, preferably a halogen; PG is an amino protecting group, preferably tert-butoxycarbonyl; and Ring B, R 1 ~R 2 , R A , X, L2 and q are as defined in general formula (III).

[0120] [Solution 6] The method for producing a compound represented by general formula (III) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0121] [ka]

[0122] a step of reacting a compound represented by general formula (IIIc) with triphenylphosphine and water to obtain a compound represented by general formula (IIIf); and a step of subjecting the compound represented by general formula (IIIf) to a condensation reaction under basic conditions to obtain a compound represented by general formula (III); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; and Ring B, R 1 ~R 2 , R A , X, L2 and q are as defined in general formula (III).

[0123] [Solution 7] The method for producing a compound represented by general formula (IV) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0124] [ka]

[0125] A step of reacting a compound represented by general formula (IIIa) with a compound represented by general formula (IVa) under basic conditions to obtain a compound represented by general formula (IVb); a step of reacting a compound represented by general formula (IVb) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (IVc); a step of subjecting a compound represented by general formula (IVc) to hydrolysis under basic conditions to obtain a compound represented by general formula (IVd); a step of further removing a protecting group PG from the compound represented by general formula (IVd) to obtain a compound represented by general formula (IVA); and a step of subjecting a compound represented by general formula (IVA) to a condensation reaction in the presence of a condensation reagent to obtain a compound represented by general formula (IV); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; R k is a leaving group, preferably a halogen; PG is an amino protecting group, preferably tert-butoxycarbonyl; and R 1 ~R 2 , G 1 , G 2 , R B , R D , R E and L2 is as defined in general formula (IV).

[0126] [Solution 8] The method for producing a compound represented by general formula (IV) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0127] [ka]

[0128] a step of reacting a compound represented by general formula (IVb) with triphenylphosphine and water to obtain a compound represented by general formula (IVe); a step of subjecting the compound represented by general formula (IVe) to a condensation reaction under basic conditions to obtain a compound represented by general formula (IV); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; and R 1 ~R 2 , G 1 , G 2 , R B , R D , R E and L2 is as defined in general formula (IV).

[0129] [Solution 9] The method for producing a compound represented by general formula (V) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0130] [ka]

[0131] A step of reacting a compound represented by general formula (IIIa) with a compound represented by general formula (Va) under basic conditions to obtain a compound represented by general formula (Vb); a step of reacting a compound represented by general formula (Vb) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (Vc); a step of subjecting a compound represented by general formula (Vc) to hydrolysis under basic conditions to obtain a compound represented by general formula (Vd); a step of further removing a protecting group PG from the compound represented by general formula (Vd) to obtain a compound represented by general formula (VA); and a step of subjecting a compound represented by general formula (VA) to a condensation reaction in the presence of a condensation reagent to obtain a compound represented by general formula (V); During the ceremony: R 3 is selected from a hydrogen atom; Rj is selected from alkyl; R k is a leaving group, preferably a halogen; PG is an amino protecting group, preferably tert-butoxycarbonyl; and R 1 ~R 2 , G 1 , G 2 , R B , R D , R E and L2 is as defined in general formula (V).

[0132] [Solution 10] The method for producing a compound represented by general formula (V) or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof according to the present invention comprises the following steps:

[0133] [ka]

[0134] a step of reacting a compound represented by general formula (Vb) with triphenylphosphine and water to obtain a compound represented by general formula (Ve); and a step of subjecting the compound represented by general formula (Ve) to a condensation reaction under basic conditions to obtain a compound represented by general formula (V); During the ceremony: R 3 is selected from a hydrogen atom; R j is selected from alkyl; and R 1 ~R 2 , G 1 , G 2 , R B , R D , R E and L2 is as defined in general formula (V).

[0135] In the above reaction conditions for preparing compounds of general formula (I), (II), (III), (IV) or (V): The basic condition is provided by an organic or inorganic base. The organic base is selected from N,N-diisopropylethylamine, pyridine, triethylamine, piperidine, N-methylpiperazine and 4-dimethylaminopyridine. The inorganic base is selected from potassium phosphate, potassium phosphate trihydrate, potassium acetate, sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, cesium carbonate, sodium hydride and potassium hydride, preferably N,N-diisopropylethylamine, sodium hydroxide, potassium hydroxide or lithium hydroxide.

[0136] The condensation reagent is selected from 2-(7-benzotriazole oxide)-N,N,N',N'-tetramethyluronium, dicyclohexylcarbodiimide, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N,N'-dicyclohexylcarbodiimide, N,N'-diisopropylcarbodiimide, 1-hydroxy-7-azobenzotriazole, 2-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate, pentafluorophenyl diphenylphosphinate, benzotriazol-1-yl-oxytris(dimethylamino)phosphonium hexahexafluorophosphate, or benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate, preferably pentafluorophenyl diphenylphosphinate.

[0137] [Mode for carrying out the invention] The following examples are used to further illustrate the present invention, but these examples are not intended to limit the scope of the present invention.

[0138] [Example] The examples show the preparation of typical compounds represented by formula (I) and the relevant structural identification data. Please note that the following examples are only used to illustrate the present invention, and are not intended to limit the present invention. 1H NMR spectra were recorded on a Bruker instrument (400 MHz) and chemical shifts are expressed in ppm with tetramethylsilane as the internal standard (0.00 ppm). 1 H NMR was designated as follows: s = singlet, d = doublet, t = triplet, m = multiplet, br = broad, dd = double doublet, dt = double triplet. Coupling constants, when given, are in Hz.

[0139] Mass spectra were measured by LC / MS, and the ionization method was either ESI or APCI.

[0140] Yantai Huanghai HSGF254 or Qingdao GF254 silica gel plates were used as silica gel plates for thin layer chromatography (TLC). The dimensions of the silica gel plates used for thin layer chromatography (TLC) were 0.15 mm to 0.2 mm, and the dimensions of the products separated and purified by TLC were 0.4 mm to 0.5 mm.

[0141] In general, 200-300 mesh Yantai Huanghai silica gel was used as the support for column chromatography.

[0142] In the following examples, unless otherwise stated, all temperatures are in degrees Celsius.Unless otherwise stated, various starting materials and reagents are commercially available or synthesized according to known methods, and commercially available materials and reagents are used directly without further purification.Unless otherwise stated, commercial manufacturers include, but are not limited to, Shanghai Hao Hong Biomedical Technology Co., Ltd., Shanghai Accela ChemBio Inc., and Shanghai Han Feng Chemical Co., Ltd., etc.

[0143] CD3OD: deuterated methanol.

[0144] CDCl3: deuterated chloroform.

[0145] DMSO-d6: deuterated dimethyl sulfoxide.

[0146] Argon atmosphere indicates that the reaction flask is connected to an argon balloon of approximately 1 L volume.

[0147] In the present examples, unless otherwise specified, the solutions refer to aqueous solutions.

[0148] The compounds are purified by the elution system of silica gel column chromatography and thin layer chromatography. The elution system is selected from A: petroleum ether and ethyl acetate system; B: dichloromethane and methanol system; and C: dichloromethane and ethyl acetate system. The volume ratio of the solvents varies according to the polarity of the compounds, and may be adjusted by adding a small amount of an acidic or basic reagent such as acetic acid or triethylamine.

[0149] Example 1 (3R)-6-Fluoro-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0150] [ka]

[0151] [ka]

[0152] (Step 1) (R,1E)-N-(3-allyl-5-fluoro-2-hydroxybenzylidene)-2-methylpropane-2-sulfenamide 3-Allyl-5-fluoro-2-hydroxybenzaldehyde 1a (2.80 g, 15.5 mmol) (prepared according to patent application WO2014022858), (R)-2-methylpropane-2-sulfinamide 1b (2.07 g, 17.1 mmol) and cesium carbonate (8.08 g, 24.8 mmol) were added to 50 mL of dichloromethane and reacted at room temperature overnight. After completion of the reaction, water (30 mL) was added to the reaction solution and extracted with dichloromethane (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R,1E)-N-(3-allyl-5-fluoro-2-hydroxybenzylidene)-2-methylpropane-2-sulfenamide 1c (4.39 g, yellow semi-solid) in a yield of 100%. MS m / z(ESI):284.1[M+1] (Step 2) (R)-N-((1R)-1-(3-allyl-5-fluoro-2-hydroxyphenyl)ethyl)-2-methylpropane-2-sulfenamide (R,1E)-N-(3-allyl-5-fluoro-2-hydroxybenzylidene)-2-methylpropane-2-sulfenamide 1c (4.39 g, 15.5 mmol) was added to 50 mL of tetrahydrofuran, and the temperature was controlled at -65°C, after which methylmagnesium bromide (77.5 ml, 77.5 mmol, 1 mol / L) was added dropwise. After the dropwise addition, the mixture was transferred to room temperature and reacted overnight. After the reaction was completed, the reaction solution was quenched with water (30 mL) and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1R)-1-(3-allyl-5-fluoro-2-hydroxyphenyl)ethyl)-2-methylpropane-2-sulfenamide 1d (1.5 g, yellow viscous substance) in a yield of 32.6%. MS m / z(ESI):300.1[M+1] (Step 3) (1R)-2-Allyl-6-(1-aminoethyl)-4-fluorophenol (R)-N-((1R)-1-(3-allyl-5-fluoro-2-hydroxyphenyl)ethyl)-2-methylpropane-2-sulfenamide 1d (0.50 g, 1.67 mmol) was added to 5 mL of dichloromethane. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 2 h. After the completion of the reaction was monitored by LC-MS, the reaction solution was filtered, the filter cake was collected, dissolved in 10 mL of water, and the pH was adjusted to neutral with sodium carbonate solution. The reaction solution was extracted with ethyl acetate (20 mL×3). Then, the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of (1R)-2-allyl-6-(1-aminoethyl)-4-fluorophenol 1e (yellow and viscous). MS m / z(ESI):178.1[M-16] (Step 4) (1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine The crude product (300 mg) of (1R)-2-allyl-6-(1-aminoethyl)-4-fluorophenol 1e was added to 5 mL of tetrahydrofuran, cooled to 0°C, and then iodosuccinimide (516 mg, 2.29 mmol) was added and reacted at room temperature overnight. After the reaction was completed, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (elution system: B) to obtain (1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 1f (100 g, brown viscous substance) in a yield of 20%. MS m / z(ESI):304.0[M-16] (Step 5) ((1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamic acid tert-butyl ester (1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 1f (500 mg, 1.56 mmol) and triethylamine (316 mg, 3.12 mmol) were dissolved in 5 mL of dichloromethane, and then di-tert-butyl dicarbonate (374 mg, 1.71 mmol) was added and reacted at room temperature for 4 hours. After the reaction was completed, the mixture was washed with saturated ammonium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated to obtain a crude product of ((1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 1g (yellow, viscous). MS m / z(ESI):422.1[M+1] (Step 6) ((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamic acid tert-butyl ester The crude product (170 mg) of ((1R)-1-(2-(iodomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 1g was added to 2 mL of N,N-dimethylformamide, and then sodium azide (32 mg, 0.49 mmol) was added and reacted at room temperature overnight. 20 mL of water was added to the reaction solution, and it was extracted with ethyl acetate (20 mL x 3). Then, the organic phase was combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product (yellow liquid) of ((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 1h. MS m / z(ESI):337.2[M+1] (Step 7) (1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine The crude product of ((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 1h (120 mg) was added to 3 mL of dichloromethane, followed by addition of 1.5 mL of hydrogen chloride-1,4-dioxane solution, and reacted at room temperature for 2 hours. The reaction solution was concentrated under reduced pressure to give the crude product of (1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 1i (yellow, viscous).

[0153] (Step 8) 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product (84 mg) of (1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 1i, N,N-diisopropylethylamine (280 mg, 2.16 mmol) and 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 1j (84 mg, 0.4 mmol) were dissolved in 3 mL of n-butyl alcohol and reacted at 120 ° C. for 5 hours. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 1k (120 mg, yellow solid). MS m / z(ESI):426.2[M+1] (Step 9) 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 1k (120 mg, 0.28 mmol) and triphenylphosphine (82 mg, 0.31 mmol) were added to 6 mL of a mixture solution (tetrahydrofuran:water=2:1) ​​and reacted at room temperature overnight. After completion of the reaction, 20 mL of water was added to the reaction solution and extracted with ethyl acetate (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 1l (76 g, yellow solid) in a yield of 67.25%. MS m / z(ESI):400.2[M+1] (Step 10) (3R)-6-Fluoro-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 1l (76 mg, 0.19 mmol) was dissolved in 3 mL of a mixture (tetrahydrofuran:methanol:water=1:4:1), lithium hydroxide monohydrate (48 mg, 1.14 mmol) was added, and the mixture was stirred at 70° C. overnight. After the reaction was monitored by LC-MS for completion, 20 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain 5-(((1R)-1-(2-(aminomethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 1m and the title product, (3R)-6-fluoro-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 1 (5 mg) was obtained in 7% yield. 1m MS m / z(ESI):372.0[M+1] 1 MS m / z(ESI):354.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.88 (d, J = 9.3 Hz, 1H), 8.65 (d, J = 4.9 Hz, 1H), 8.55 (d, J = 7.6 Hz, 1H), 8.01 (s, 1H), 6.84 (dd, J = 8.5, 2.5 Hz, 1H), 6.75 (dd, J = 9.9, 2.7 Hz, 1H), 6.38 (d, J = 7.6 Hz, 1H), 5.36 (dt, J = 10.0, 4.7 Hz, 1H), 4.92-5.04 (m, 1H), 3.56-3.70 (m, 2H), 3.50 (dd, J = 13.4, 4.9 Hz, 1H), 2.83 (dd, J = 16.9, 4.8 Hz, 1H), 1.55 (d, J = 7.0 Hz, 3H). Example 2 (12S)-6-Fluoro-3-methyl-10,24-dioxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,12 .0 4,9 .0 19,23 ]Tetracosa-1(22),4(9),5,7,16(23),17,20-heptaen-15-one

[0154] [ka]

[0155] [ka]

[0156] (Step 1) 1-(5-fluoro-2,3-dihydroxyphenyl)ethan-1-one 4-Fluoro-2-methoxyphenol 2a (13.5 g, 95 mmol) was added to 200 mL of boron trifluoride-acetic acid complex, heated to 135 °C, and reacted for 16 hours. After completion of the reaction, 100 mL of water was added to the reaction solution, and extracted with ethyl acetate (80 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain 1-(5-fluoro-2,3-dihydroxyphenyl)ethan-1-one 2b (1.2 g, yellow solid) in a yield of 7.5%. MS m / z(ESI):171.0[M+1] (Step 2) (2S)-1-(7-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one Sodium hydroxide (564 mg, 14.1 mmol) was dissolved in 20 mL of water, and 1-(5-fluoro-2,3-dihydroxyphenyl)ethan-1-one 2b (1.2 g, 7.05 mmol) and (R)-2-(chloromethyl)oxirane were added, heated to 105 ° C, and reacted overnight. After completion of the reaction, the reaction solution was cooled, 20 mL of water was added to the reaction solution, and extracted with ethyl acetate (30 mL × 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain (2S)-1-(7-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 2c (320 mg, yellow viscous substance) in a yield of 20.5%. MS m / z(ESI):227.0[M+1] (Step 3) (2R)-(5-acetyl-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonate (2S)-1-(7-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 2c (320 mg, 1.41 mmol) was added to 1.5 mL of dichloromethane, cooled to 0°C, 4-dimethylaminopyridine (350 mg, 2.86 mmol) was added, methylsulfonyl chloride (243 mg, 2.12 mmol) was added dropwise, and the mixture was allowed to react at room temperature overnight. After the reaction was completed, 30 mL of saturated ammonium chloride solution was added, extracted with dichloromethane (30 mL x 3), and washed with saturated ammonium chloride solution. The reaction mixture was then dried over anhydrous sodium sulfate and concentrated under reduced pressure to give the crude product (2R)-(5-acetyl-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonic acid 2d (430 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):305.0[M+1] (Step 4) (2S)-1-(2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one (2R)-(5-acetyl-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonate 2d (430 mg) was added to 10 mL of N,N-dimethylformamide solution, then sodium azide (193 mg, 2.97 mmol) was added, and the mixture was heated to 55° C. and reacted overnight. After completion of the reaction, 30 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (30 mL×3). Then, the organic phases were combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (2S)-1-(2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 2e (230 g, yellow semi-solid) in 65% yield. MS m / z(ESI):252.0[M+1] (Step 5) 1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine (2S)-1-(2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 2e (230 mg, 0.92 mmol), ammonium acetate (710 mg, 9.2 mmol) and sodium cyanoborohydride (116 mg, 1.84 mmol) were added to 5 mL of methanol, reacted at room temperature for 1 hour, heated to reflux, and then reacted overnight. The reaction solution was concentrated under reduced pressure, 30 mL of water was added, and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 230 mg of crude 1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 2f. The product was used in the next reaction without purification. MS m / z(ESI):253.1[M+1] (Step 6) 5-((1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazole[1,5]-a]pyrimidine-3-carboxylate ethyl The crude product of 1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 2f (230 mg), N,N-diisopropylethylamine (95 mg, 0.74 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (248 mg, 1.10 mmol) were dissolved in 5 mL of n-butanol and reacted at 120° C. for 5 hours. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-((1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazole[1,5]-a]pyrimidine-3-carboxylate 2g (50 mg, yellow semi-solid) in a yield of 12.4%. MS m / z(ESI):442.2[M+1] (Step 7) 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 2g (50mg, 0.11mmol) of 5-((1-((2S)-2-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazole[1,5]-a]pyrimidine-3-carboxylate ethyl was added to 5mL of methanol. Then, di-tert-butyl dicarbonate (30mg, 0.14mmol) and 10mg of 10% palladium carbon were added, and the mixture was reacted at room temperature for 5 hours under the protection of hydrogen. The reaction mixture was filtered, and the filtrate was directly concentrated to give a crude product of ethyl 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 2h (58 mg, yellow viscous substance). This product was used in the next reaction without purification. MS m / z(ESI):516.2[M+1] (Step 8) 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of 2h (58 mg) was dissolved in 3 mL of a mixed solution (tetrahydrofuran:methanol:water = 1:4:1), lithium hydroxide monohydrate (46 mg, 1.10 mmol) was added, and the mixture was stirred at 75 ° C. overnight. After the reaction was completed, 20 mL of water was added and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give crude 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 2i (54 mg, yellow viscous material), which was used in the next reaction without purification. MS m / z(ESI):488.2[M+1] (Step 9) 5-((1-((2S)-2-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid The crude product of 5-((1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 2i (54 mg) was added to 2 mL of dichloromethane, and 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. The reaction solution was concentrated under reduced pressure to obtain the crude product of 5-((1-((2S)-2-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 2j. The product was used in the next reaction without purification. MS m / z(ESI):388.1[M+1] (Step 10) (12S)-6-Fluoro-3-methyl-10,24-dioxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,12 .0 4,9 .0 19,23 ]Tetracosa-1(22),4(9),5,7,16(23),17,20-heptaen-15-one The crude product of 5-((1-((2S)-2-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 2j (43 mg), pentafluorobenzene diphenyl phosphate (51 mg, 0.13 mmol) and N,N-diisopropylethylamine (142 mg, 1.10 mmol) were dissolved in 4 mL of a mixture (dichloromethane:N-dimethylformamide = 3:1) and reacted at room temperature overnight. After the reaction was completed, 20 mL of saturated aqueous ammonium chloride solution was added and extracted with dichloromethane (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (12S)-6-fluoro-3-methyl-10,24-dioxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,12 .0 4,9 .0 19,23 ]Tetracosa-1(22),4(9),5,7,16(23),17,20-heptaen-15-one 2 (3 mg) was obtained in 7.5% yield. MS m / z(ESI):370.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.56 (d, J = 7.6 Hz, 1H), 8.52 (d, J = 3.8 Hz, 1H), 8.08 (d, J = 6.4 Hz, 2H), 6.65 (dd, J = 9.6, 3.0 Hz, 1H), 6.56 (dd, J = 9.2, 3. 0 Hz, 1H), 6.43 (d, J = 7.6 Hz, 1H), 5.16-5.21 (m, 1H), 4.80-4.86 (m, 2H), 4.35-4.38 (m, 1H), 4.16-4.22 (m, 2H), 3.55 (d, J = 14.8 Hz, 1H), 1.57 (d, J = 7.0 Hz, 3H). [Examples 3 and 4] (3R,11R)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 3 (3R,11S)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 4

[0157] [ka]

[0158] [ka]

[0159] (Step 1) (1R,2E)-4-(3-(1-(tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-hydroxyphenyl)but-2-enoic acid ethyl ester (1R)-(3-allyl-5-fluoro-2-hydroxyphenyl)ethyl)carbamate tert-butyl 3a (2.95 g, 10 mmol), ethyl acrylate (2.5 g, 25 mmol), benzylidene-bis(tricyclohexylphosphine)dichlororuthenium (170 mg, 0.2 mmol) and cuprous iodide (40 mg, 0.2 mmol) were added to 50 mL of diethyl ether, heated to 40 ° C. and reacted overnight. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A), to obtain (1R,2E)-4-(3-(1-(tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-hydroxyphenyl)but-2-enoate ethyl 3b (2.3 g, yellow liquid) in a yield of 57.07%. MS m / z(ESI):368.2[M+1] (Step 2) 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl acetate (1R,2E)-4-(3-(1-(tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-hydroxyphenyl)but-2-enoic acid ethyl ester 3b (2.18 g, 5.95 mmol) and cesium carbonate (1.94 g, 5.95 mmol) were dissolved in 4 mL of acetonitrile and reacted at room temperature overnight. After completion of the reaction, 20 mL of water was added and extracted with ethyl acetate (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl acetate 3c (1.18 g, yellow solid) in 53.63% yield. MS m / z(ESI):368.2[M+1] (Step 3) ((1R)-1-(5-fluoro-2-(2-hydroxyethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)carbamic acid tert-butyl ester 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl acetate 3c (1.18 g, 3.2 mmol) was dissolved in 15 mL of dichloromethane and cooled to −78° C. under the protection of argon, and diisobutylaluminum hydride (3.84 mL, 3.84 mmol, 1 M) was added dropwise. After the addition, the mixture was reacted at room temperature. After the reaction was completed, the reaction solution was quenched with 30 mL of water and extracted with ethyl acetate (20 mL×3). Then, the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of ((1R)-1-(5-fluoro-2-(2-hydroxyethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)tert-butyl carbamate 3d (yellow, viscous). MS m / z(ESI):326.2[M+1] (Step 4) 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl methanesulfonate ((1R)-1-(5-fluoro-2-(2-hydroxyethyl)-2,3-dihydrobenzofuran-7-yl)ethyl) tert-butyl carbamate 3d (1.05 g) and triethylamine (1.34 mL, 9.6 mmol) were dissolved in 20 mL of dichloromethane, and methylsulfonyl chloride (440 mg, 3.84 mmol) was slowly added dropwise at 0° C. and reacted at room temperature for 2 hours. After completion of the reaction, the reaction solution was quenched with 30 mL of water and extracted with ethyl acetate (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl methanesulfonate 3e (yellow, viscous). MS m / z(ESI):404.2[M+1] (Step 5) ((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamic acid tert-butyl ester The crude product (300 mg) of 2-(7-((1R)-1-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-2-yl)ethyl methanesulfonate 3e was added to 3 mL of N,N-dimethylformamide solution. Sodium azide (64 mg, 0.98 mmol) was then added and reacted at room temperature overnight. After the reaction was completed, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product (yellow liquid) of ((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 3f. MS m / z(ESI):351.2[M+1] (Step 6) (1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine The crude product of ((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)carbamate tert-butyl 3f was added to 5mL of dichloromethane. Then, 2mL of hydrogen chloride·1,4-dioxane solution (4mol / L) was added and reacted at room temperature for 2 hours. After the reaction was completed, the reaction solution was directly concentrated under reduced pressure to obtain the crude product of (1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 3g. MS M / z(ESI):233.1[M-16] (Step 7) 5-(((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of (1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 3g (193 mg), N,N-diisopropylethylamine (597 mg, 4.62 mmol) and 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 1j (182 mg, 0.81 mmol) were dissolved in 5 mL of n-butanol and reacted at 120 ° C. for 5 hours. After the reaction was completed, the reaction solution was directly concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 3h. MS m / z(ESI):440.2[M+1] (Step 8) 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of 5-(((1R)-1-(2-(2-azidoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 3h and triphenylphosphine (200 mg, 0.92 mmol) were added to 6 mL of a mixed solution (tetrahydrofuran: water = 2: 1) and reacted at room temperature overnight. The reaction solution was concentrated under reduced pressure, and the obtained residue was further separated and purified by silica gel column chromatography (eluent: A), and 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 3i (280 mg, yellow foam solid) was obtained in a yield of 88%. MS m / z(ESI):414.2[M+1] (Step 9) 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl Ethyl 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 3i (110 mg, 0.27 mmol) and triethylamine (55 mg, 0.54 mmol) were dissolved in 5 mL of dichloromethane. Di-tert-butyl dicarbonate (69 mg, 0.32 mmol) was then added and the mixture was allowed to react at room temperature for 3 hours. After completion of the reaction, the reaction solution was washed with saturated ammonium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to obtain the crude product of ethyl 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 3j (yellow liquid). MS m / z(ESI):514.2[M+1] (Step 10) 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 3j (100 mg) was dissolved in 5 mL of a mixture (tetrahydrofuran:methanol:water=1:3:1), lithium hydroxide monohydrate (50 mg, 1.19 mmol) was added, and the mixture was stirred at 70° C. overnight. After completion of the reaction, 20 mL of water was added, and the mixture was extracted with ethyl acetate (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the crude product of 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 3k. MS m / z(ESI):486.2[M+1] (Step 11) 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 3k (80 mg) was added to 3 mL of dichloromethane. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 2 hours. The reaction solution was concentrated under reduced pressure to obtain a crude product of 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 3l. MS m / z(ESI):386.2[M+1] (Step 12) (3R,11R)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 3 (3R,11S)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 4 5-(((1R)-1-(2-(2-aminoethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 3l, pentafluorophenyl diphenylphosphinate (97 mg, 0.19 mmol) and N,N-diisopropylethylamine (170 mg, 1.31 mmol) were dissolved in 5 mL of a mixed solution (dichloromethane: N-N-dimethylformamide = 1.5: 1) and reacted at room temperature overnight. After the reaction was completed, 20 mL of water was added to the reaction solution and extracted with dichloromethane (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (3R,11R)-6-fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 3 (8 mg) and (3R,11S)-6-fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1 (22),4,6,8,16 (23),17,20-heptaen-15-one 4 (6 mg) was obtained. 3 MS m / z(ESI):368.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.64 (s, 1H), 8.16 (s, 1H), 8.04 (d, J = 17.2 Hz, 1H), 7.63-7.72 (m, 1H), 6.78 (t, J = 9.5 Hz, 1H), 6.68 (t, J = 7.7 Hz, 1H), 6.28 (t, J = 7.3 Hz, 1H), 5.32 (d, J = 8.6 Hz, 1H), 5.07 (s, 1H), 3.94-4.30 (m, 1H), 3.31 (dt, J = 9.9, 5.5 Hz, 2H), 2.90 (dt, J = 16.1, 8.0 Hz, 1H), 2.10-2.18 (m, 1H), 1.71-1.75 (m, 1H), 1.42 (d, J = 6.3 Hz, 3H). 4 MS m / z(ESI):368.3[M+1] 1 H NMR (400 MHz, DMSO-d6) δ 8.90 (d, J = 9.1 Hz, 1H), 8.52 (d, J = 7.6 Hz, 1H), 8.43 (d, J = 8.3 Hz, 1H), 8.08 (s, 1H), 6.98 (t, J = 9.3 Hz, 2H), 6.28 (d, J = 7.1 Hz, 1H), 5.55 (q, J = 7.6 Hz, 1H), 4.37-4.42 (m, 1H), 4.11 (d, J = 11.6 Hz, 1H), 3.22-3.24 (m, 1H), 2.99 (dd, J = 14.7, 6.3 Hz, 1H), 2.87 (t, J = 13.9 Hz, 1H), 2.18 (d, J = 11.5 Hz, 1H), 1.97- 2.12 (m, 1H), 1.54 (d, J = 7.1 Hz, 3H). [Example 5] (3R)-6-フルオロ-3,11-ジメチル-10-オキサ-2,13,17,18,21-ペンタアザペンタシクロ[13.5.2.1 8,11 .0 4,9 .0 18,22]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0160] [ka]

[0161] [ka]

[0162] (Step 1) 1-(5-Fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-fluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 5a (12 g, 57.67 mmol) (prepared according to patent application WO2014022858) was added to 75 mL of tetrahydrofuran. Then, iodosuccinimide (25.95 g, 115.33 mmol) was added and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution and extracted with ethyl acetate (50 mL x 3). Then, the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain 1-(5-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5b (15.2 g, yellow solid) in a yield of 78.9%. MS m / z(ESI):335.0[M+1] (Step 2) 1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5b (3 g, 8.98 mmol) was added to 20 mL of N,N-dimethylformamide and stirred to dissolve. Sodium azide (1.17 g, 17.96 mmol) was then added, heated to 75 ° C, and reacted overnight. After the reaction was completed, 60 mL of water was added to the reaction solution and extracted with ethyl acetate (50 mL × 3). The organic phases were then combined, washed three times with 30 mL of water, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain 1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5c (1.2 g, yellow liquid) in a yield of 60%. MS m / z(ESI):250.1[M+1] (Step 3) (R)-N-((1E)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfenamide 1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5c (1.2 g, 4.8 mmol), (R)-2-methylpropane-2-sulfinamide 1b (1.17 g, 9.6 mmol) and tetraethyl titanate (4.4 g, 19.3 mmol) were dissolved in 20 mL of tetrahydrofuran and reacted at 75° C. overnight. After completion of the reaction, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1E)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfenamide 5d (1.02 g, yellow liquid) in a yield of 60.3%. MS m / z(ESI):353.1[M+1] (Step 4) (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfenamide (R)-N-((1E)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfenamide 5d (1.02 g, 2.90 mmol) was added to 20 mL of tetrahydrofuran, and 9-boron bicyclo[3,3,1]-nonane (11.6 mL, 5.80 mmol, 0.5 mol / L) was added and reacted at room temperature for 4 hours. After completion of the reaction, the reaction solution was quenched with 30 mL of water and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to give (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfenamide. 5e (840 mg, yellow solid) was obtained in 81.9% yield. MS m / z(ESI):355.1[M+1] (Step 5) (1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfenamide 5e (840 mg, 2.37 mmol) was added to 5 mL of dichloromethane solution. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 2 hours. After completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of (1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 5f. The product was used in the next reaction without purification. MS m / z(ESI):234.1[M-16] (Step 6) 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of (1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 5f (593 mg), ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (533 mg, 2.37 mmol) and N,N-diisopropylethylamine (2.45 g, 18.96 mmol) were dissolved in 5 mL of n-butanol and reacted at 125 ° C for 3 hours. After the completion of the reaction was monitored by LC-MS, 20 mL of water was added to the reaction solution and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 5 g of crude ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate, which was used in the next reaction without purification. MS m / z(ESI):440.1[M+1] (Step 7) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5g (410mg, 0.93mmol)) was added to 5mL of methanol. Then, di-tert-butyl dicarbonate (244mg, 1.12mmol) and 100mg of 10% palladium carbon were added, and the mixture was reacted at room temperature for 4 hours under hydrogen protection. After the reaction was completed, the reaction solution was filtered, and the filtrate was directly concentrated to obtain the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]]pyrimidine-3-carboxylate ethyl 5h. The product was used in the next reaction without purification. MS m / z(ESI):514.2[M+1] (Step 8) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]]pyrimidine-3-carboxylate ethyl The crude product of 5h (479 mg) was dissolved in 6 mL of a mixture solution (tetrahydrofuran:ethanol:water:2:2:1), lithium hydroxide monohydrate (310 mg, 7.39 mmol) was added, and the mixture was stirred at 80 ° C. overnight. After the completion of the reaction was monitored by LC-MS, the reaction solution was cooled and diluted hydrochloric acid was slowly added dropwise to adjust it to acidity, then 10 mL of water was added and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5i, which was used in the next reaction without purification. MS m / z(ESI):486.2[M+1] (Step 9) 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5i (451 mg) was added to 10 mL of dichloromethane solution. Then, 3 mL of hydrogen chloride·1,4-dioxane solution was added and reacted at room temperature for 1 hour. After completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5j. The product was used in the next reaction without purification. MS m / z(ESI):386.1[M+1] (Step 10) (3R)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5j (358 mg), pentafluorophenyl diphenylphosphinate (428 mg, 1.12 mmol) and N,N-diisopropylethylamine (961 mg, 7.44 mmol) were dissolved in 6 mL of a mixture (dichloromethane: N-N-dimethylformamide = 5:1) and reacted at room temperature overnight. After the completion of the reaction was monitored by LC-MS, 20 mL of saturated aqueous ammonium chloride was added to the reaction solution and extracted with dichloromethane (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (3R)-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 5 (20 mg) was obtained. MS m / z(ESI):368.1[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.60 (d, J = 7.2 Hz, 1H), 8.51 (d, J = 7.6 Hz, 1H), 8.16 (s, 1H), 8.04 (s, 1H), 7.22-7.30 (m, 1H), 7.02-7.14 (m, 1H), 6.72 (s, 1H), 6.34 (d, J = 7.7 Hz, 1H), 5.74 (t, J = 7.2 Hz, 1H), 3.74 (dd, J = 13.0, 5.5 Hz, 1H), 3.46-3.59 (m, 1H), 3.24 (dd, J = 15.4, 5.4 Hz, 1H), 3.19-3.04 (m, 1H), 1.64 (d, J = 7.1 Hz, 3H). Example 6 (3R)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,11(24),16(23),17,20-octaen-15-one

[0163] [ka]

[0164] [ka]

[0165] (Step 1) 4-Fluoro-2,6-diiodophenol 4-Fluorophenol 6a (1.12 g, 0.01 mol), iodine (3.81 g, 0.015 mol) and potassium iodide (2.50 g, 0.015 mol) were added to 50 mL of water, cooled to 0°C, sodium hydroxide solution (800 mg, 0.02 mol, 50 mL of water) was added dropwise, and the mixture was transferred to room temperature and reacted overnight. After the reaction was completed, the pH of the solution was adjusted to weak acidity with dilute hydrochloric acid and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to obtain 4-fluoro-2,6-diiodophenol 6b (3.5 g, brown viscous substance) in a yield of 96.25%. MS m / z(ESI):364.8[M+1] (Step 2) 2-(5-Fluoro-7-iodobenzofuran-2-yl)ethan-1-ol 4-Fluoro-2,6-diiodophenol 6b (960 mg, 2.64 mmol), but-3-yn-1-ol 6c (185 mg, 2.64 mmol), potassium acetate (778 mg, 7.92 mmol), triphenylphosphine (104 mg, 0.40 mmol), palladium acetate (30 mg, 0.13 mmol) and cuprous iodide (50 mg, 0.26 mmol) were added to 10 mL of acetonitrile, heated to 80 ° C under argon protection, and reacted overnight. The reaction solution was concentrated under reduced pressure, and the obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain 2-(5-fluoro-7-iodobenzofuran-2-yl)ethan-1-ol 6d (510 mg, yellow solid) in 63.1% yield. MS m / z(ESI):307.0[M+1] (Step 3) 2-(5-fluoro-7-iodobenzofuran-2-yl)ethyl methanesulfonate 2-(5-fluoro-7-iodobenzofuran-2-yl)ethan-1-ol 6d (6.7 g, 21.9 mmol) was dissolved in 70 mL of dichloromethane, triethylamine (6.1 mL, 44.0 mmol) was added, methanesulfonyl chloride (3.0 g, 26.2 mmol) was slowly added dropwise at 0 ° C, and the mixture was transferred to room temperature and reacted for 4 hours. After the reaction was completed, the reaction solution was quenched with 50 mL of saturated aqueous ammonium chloride solution and extracted with dichloromethane (30 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 2-(5-fluoro-7-iodobenzofuran-2-yl)ethyl methanesulfonate 6e (7.7 g, yellow liquid). MS m / z(ESI):384.9[M+1] (Step 4) 2-(2-azidoethyl)-5-fluoro-7-iodobenzofuran The crude product (7.7 g) of 2-(5-fluoro-7-iodobenzofuran-2-yl)ethyl methanesulfonate 6e was added to 60 mL of N,N-dimethylformamide solution. Sodium azide (1.56 g, 24.0 mmol) was then added and reacted at room temperature overnight. 150 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (80 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product (yellow liquid) of 2-(2-azidoethyl)-5-fluoro-7-iodobenzofuran 6f. MS m / z(ESI):332.0[M+1] (Step 5) 1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-one 2-(2-azidoethyl)-5-fluoro-7-iodobenzofuran 6f, tributyl-(1-ethoxyvinyl)stannane (2.78 g, 7.7 mmol) and dichlorobu(triphenylphosphine)palladium (250 mg, 0.36 mmol) were added to 25 mL of 1,4-dioxane, heated to 90 ° C under the protection of argon, and reacted overnight. After the reaction was completed, the reaction solution was cooled to room temperature, 10% potassium fluoride aqueous solution (405 mg, 7 mmol) was added, and stirred at room temperature for 2 hours. After filtering the reaction solution, the filtrate was concentrated, and 10 mL of 1 mol / L dilute hydrochloric acid was added and stirred at room temperature for 2 hours. The pH of the solution was adjusted to basic with saturated sodium hydroxide solution, and then extracted with ethyl acetate (30 mL × 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain 1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-one 6g (580 mg, yellow liquid) in a yield of 33.7%. MS m / z(ESI):248.0[M+1] (Step 6) (R,1E)-N-(1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-one 6g (580 mg, 2.35 mmol), (R)-2-methylpropane-2-sulfinamide 1b (287 mg, 2.37 mmol) and tetraethyl titanate (1.07 g, 4.69 mmol) were dissolved in 8 mL of tetrahydrofuran and reacted at 75 ° C. overnight. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A), and (R, 1E)-N-(1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 6h (750 mg, yellow semi-solid) was obtained in a yield of 91.2%. MS m / z(ESI):351.1[M+1] (Step 7) (R)-N-((1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R,1E)-N-(1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 6h (750 mg, 2.14 mmol) was dissolved in 15 mL of methanol, sodium borohydride (162 mg, 4.28 mmol) was added, and the mixture was reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 50 mL of water and extracted with ethyl acetate (30 mL x 3). Then, the organic phases were combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain (R)-N-((1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 6i (262 mg, yellow semi-solid) in a yield of 34.7%. MS m / z(ESI):353.1[M+1] (Step 8) (1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 6i (262 mg, 0.74 mmol) was added to 2 mL of dichloromethane. Then, 3 mL of hydrogen chloride-1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 183 mg of crude product of (1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-amine 6j. The product was used in the next reaction without purification.

[0166] (Step 9) (1R)-5-((1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product (1R)-1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethan-1-amine 6j (183 mg), N,N-diisopropylethylamine (597 mg, 4.62 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (200 mg, 0.89 mmol) were dissolved in 3 mL of n-butanol and reacted at 120 ° C. for 6 hours. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A), to obtain ethyl (1R)-5-((1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 6k (300 mg, yellow solid) in a yield of 92.8%. MS m / z(ESI):438.2[M+1] (Step 10) (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-5-((1-(2-(2-azidoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 6k (300 mg, 0.69 mmol) was added to 5 mL of methanol. Then, di-tert-butyl dicarbonate (180 mg, 0.82 mmol) and 60 mg of 10% palladium carbon were added, and the mixture was reacted at room temperature overnight under hydrogen protection. After the reaction was completed, the reaction solution was filtered, and the filtrate was directly concentrated to obtain a crude product of (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 6l (353 mg, yellow viscous substance). The product was used in the next reaction without purification. MS m / z(ESI):512.2[M+1] (Step 11) (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid The crude product (353 mg) of (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 6l was dissolved in 5 mL of a mixture (tetrahydrofuran:methanol:water=1:3:1), and lithium hydroxide monohydrate (290 mg, 6.9 mmol) was added and stirred at 70°C overnight. After the reaction was completed, 20 mL of water was added and extracted with ethyl acetate (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain a crude product of (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 6m, which was used in the next reaction without purification. MS m / z(ESI):484.2[M+1] (Step 12) (1R)-5-((1-(2-(2-aminoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (1R)-5-((1-(2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 6m was added to 2mL of dichloromethane, and 4mL of hydrogen chloride·1,4-dioxane solution (4mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was directly concentrated under reduced pressure to obtain the crude product of (1R)-5-((1-(2-(2-aminoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 6n. MS m / z(ESI):384.1[M+1] (Step 13) (3R)-6-Fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,11(24),16(23),17,20-octaen-15-one (1R)-5-((1-(2-(2-aminoethyl)-5-fluorobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 6n (265 mg), pentafluorophenyl diphenylphosphinate (318 mg, 0.83 mmol) and N,N-diisopropylethylamine (713 mg, 5.52 mmol) were dissolved in 4 mL of a mixed solution (dichloromethane: N-N-dimethylformamide = 3:1) and reacted at room temperature overnight. After the reaction was completed, 20 mL of saturated aqueous ammonium chloride solution was added and extracted with dichloromethane (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (3R)-6-fluoro-3-methyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,11(24),16(23),17,20-octaen-15-one 6 (64 mg) was obtained in 25.4% yield. MS m / z(ESI):366.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.60 (d, J = 7.2 Hz, 1H), 8.51 (d, J = 7.6 Hz, 1H), 8.16 (s, 1H), 8.04 (s, 1H), 7.22-7.30 (m, 1H), 7.02-7.14 (m, 1H), 6.72 (s, 1H), 6.34 (d, J = 7.7 Hz, 1H), 5.74 (t, J = 7.2 Hz, 1H), 3.74 (dd, J = 13.0, 5.5 Hz, 1H), 3.46-3.59 (m, 1H), 3.24 (dd, J = 15.4, 5.4 Hz, 1H), 3.19-3.04 (m, 1H), 1.64 (d, J = 7.1 Hz, 3H). Example 7 (2R,14R)-19-Fluoro-2-methyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one

[0167] [ka]

[0168] [ka]

[0169] (3R)-1-(7-fluoro-3-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one Sodium hydroxide (564 mg, 14.1 mmol) was dissolved in 20 mL of water, 1-(5-fluoro-2,3-dihydroxyphenyl)ethan-1-one 2b (1.2 g, 7.05 mmol) and (R)-2-(chloromethyl)ethylene oxide were added, and the mixture was heated to 105 ° C. and reacted overnight. After the reaction was completed, 20 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (30 mL × 3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain (3R)-1-(7-fluoro-3-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 7a (320 mg, yellow dope) in a yield of 20.5%. MS m / z(ESI):227.0[M+1] (Step 2) (2S)-(8-Acetyl-6-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonate (3R)-1-(7-fluoro-3-(hydroxymethyl)-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 7a (320 mg, 1.41 mmol) was added to 1.5 mL of dichloromethane, cooled to 0°C, 4-dimethylaminopyridine (350 mg, 2.86 mmol) was added, methylsulfonyl chloride (243 mg, 2.12 mmol) was added dropwise, and the mixture was allowed to react at room temperature overnight. After the reaction was completed, 30 mL of saturated ammonium chloride solution was added and extracted with dichloromethane (30 mL x 3). The organic phases were then combined, washed with saturated ammonium chloride solution, dried over anhydrous sodium sulfate, and the reaction solution was concentrated under reduced pressure to give crude (2S)-(8-acetyl-6-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonic acid 7b (430 mg, yellow viscous material) in 100% yield. MS m / z(ESI):305.0[M+1] (Step 3) (3R)-1-(3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one (2S)-(8-acetyl-6-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-2-yl)methyl methanesulfonate 7b (430 mg, 1.41 mmol) was added to 10 mL of N,N-dimethylformamide solution. Sodium azide (193 mg, 2.97 mmol) was then added and the mixture was heated to 55° C. and reacted overnight. After completion of the reaction, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (3R)-1-(3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 7c (230 g, yellow semi-solid) in a yield of 65%. MS m / z(ESI):252.0[M+1] (Step 4) (R)-N-((1E)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylidene)-2-methylpropane-2-sulfinamide (3R)-1-(3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 7c (230 mg, 0.92 mmol), (R)-2-methylpropane-2-sulfinamide 1b (333 mg, 2.75 mmol) and tetraethyl titanate (836 mg, 3.66 mmol) were dissolved in 5 mL of tetrahydrofuran and reacted at 75° C. overnight. The reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1E)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylidene)-2-methylpropane-2-sulfinamide 7d (218 mg, yellow solid) in a yield of 66.9%. MS m / z(ESI):355.1[M+1] (Step 5) (R)-N-((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((1E)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylidene)-2-methylpropane-2-sulfinamide 7d (1.0 g, 2.82 mmol) was added to 10 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (11.3 mL, 5.65 mmol, 0.5 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction was quenched with 30 mL of water and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the crude product of (R)-N-((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)-2-methylpropane-2-sulfinamide 7e (1.0 g, yellow gum) in 100% yield. MS m / z(ESI):357.1[M+1] (Step 6) (1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine The crude product (1.0 g) of (R)-N-((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)-2-methylpropane-2-sulfinamide 7e was added to 10 mL of dichloromethane, and 10 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain the crude product (708 mg) of (1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 7f in a yield of 100%. The product was used in the next reaction without purification. MS m / z(ESI):253.1[M+1] (Step 7) 5-(((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product (708 mg) of (1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 7f, N,N-diisopropylethylamine (3.62 g, 28.0 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (695 mg, 3.09 mmol) were dissolved in 10 mL of n-butanol and reacted at 120° C. for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure, and the obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 7g (570 mg, yellow viscous substance) in a yield of 46%. MS m / z(ESI):442.2[M+1] (Step 8) 5-(((1R)-1-((3R)-3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-((3R)-3-(azidomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 7g (570mg, 1.29mmol) was added to 5mL of methanol. Then, di-tert-butyl dicarbonate (338mg, 1.55mmol) and 100mg of 10% palladium carbon were added, and the mixture was reacted at room temperature for 5 hours under the protection of hydrogen. After completion of the reaction, the reaction solution was filtered, and the filtrate was directly concentrated to obtain the crude product of ethyl 5-(((1R)-1-((3R)-3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 7h (666 mg, yellow viscous substance) in 100% yield. The product was used in the next reaction without purification. MS m / z(ESI):516.2[M+1] (Step 9) The crude product (666 mg) of 5-(((1R)-1-((3R)-3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 7h was dissolved in 5 mL of a mixture solution (tetrahydrofuran:methanol:water=1:3:1), lithium hydroxide monohydrate (845 mg, 13.0 mmol) was added, and the mixture was stirred at 75° C. overnight. After the reaction was completed, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product 5-(((1R)-1-((3R)-3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 7i in 100% yield. The product was used in the next reaction without purification. MS m / z(ESI):488.2[M+1] (Step 10) 5-(((1R)-1-((3R)-3-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid The crude product of 5-(((1R)-1-((3R)-3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 7i (630 mg) was added to 3 mL of dichloromethane, and 5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. The reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-((3R)-3-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 7j (500 mg) in 100% yield. MS m / z(ESI):388.1[M+1] (Step 11) (2R,14R)-19-Fluoro-2-methyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one The crude product of 5-(((1R)-1-((3R)-3-(aminomethyl)-7-fluoro-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 7j (500 mg), pentafluorophenyl diphenylphosphinate (600 mg, 1.56 mmol) and N,N-diisopropylethylamine (1.35 g, 10.44 mmol) were dissolved in 6 mL of a mixture (dichloromethane: N,N-dimethylformamide = 5:1) and reacted at room temperature overnight. After the reaction was completed, 20 mL of saturated aqueous ammonium chloride solution was added and extracted with dichloromethane (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (2R,14R)-19-fluoro-2-methyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2]. 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 7 (200 mg) was obtained in 42% yield. MS m / z(ESI):370.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 9.39 (t, J = 4.9 Hz, 1H), 8.74 (d, J = 6.0 Hz, 1H), 8.58 (d, J = 7.6 Hz, 1H), 8.05 (s, 1H), 6.68 (ddd, J = 14.1, 9.4, 3.0 Hz, 2H), 6.38 (d, J = 7.6 Hz, 1H), 5.45 (q, J = 6.9 Hz, 1H), 4.91 (dt, J = 9.3, 4.5 Hz, 1H), 4.38 (dd, J = 11.8, 3.3 Hz, 1H), 3.90 (dd, J = 11.8, 5.4 Hz, 1H), 3.67 (dt, J = 13.7, 5.5 Hz, 1H), 3.48 (dt, J = 13.5, 5.5 Hz, 1H), 1.47 (d, J = 7.0 Hz, 3H). Example 8 (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0170] [ka]

[0171] [ka]

[0172] (Step 1) (R)-N-((1E)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 8a (R)-N-((1E)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 8b 1-(2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5c (1.92 g, 7.71 mmol), (R)-2-methylpropane-2-sulfinamide 1b (1.87 g, 15.4 mmol) and tetraethyl titanate (7.03 g, 30.8 mmol) were dissolved in 20 mL of tetrahydrofuran and reacted at 75° C. overnight. After completion of the reaction, 30 mL of water was added to the reaction solution and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: petroleum ether: ethyl acetate = 10: 1) to obtain the first eluted (R)-N-((1E)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl) ethylidene)-2-methylpropane-2-sulfinamide 8a (1.32 g, yellow liquid, yield: 48.8%), and the gradually eluted (R)-N-((1E)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl) ethylidene)-2-methylpropane-2-sulfinamide 8b (1.02 g, yellow liquid, yield 37.4%). MS m / z(ESI):353.2[M+1] (Step 2) (R)-N-((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((1E)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 8a (1.32 g, 3.77 mmol) was added to 20 mL of tetrahydrofuran. Then, 9-boron bicyclo[3,3,1]-nonane (15.08 mL, 7.54 mmol, 0.5 mol / L) was added and reacted at room temperature for 4 hours. After completion of the reaction, the reaction solution was quenched with 30 mL of water and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 8c (1.22 g, yellow solid) in a yield of 91.1%. MS m / z(ESI):355.1[M+1] (Step 3) (1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 8c (1.22 g, 3.46 mmol) was added to 5 mL of dichloromethane solution. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of (1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 8d. The product was used in the next reaction without purification. MS m / z(ESI):234.1[M-16] (Step 4) 5-(((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of (1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 8d (866 mg), ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (810 mg, 3.46 mmol) and N,N-diisopropylethylamine (3.58 g, 27.68 mmol) were dissolved in 10 mL of n-butanol and reacted at 125° C. for 3 hours. After the completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure, and the obtained residue was further separated and purified by silica gel column chromatography (elution system: A) to give ethyl 5-(((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 8e (910 mg, slightly yellow solid) in 59.8% yield. MS m / z(ESI):440.0[M+1] (Step 5) 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-((2R)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 8e (910 mg, 2.07 mmol) was added to 10 mL of methanol. Then, di-tert-butyl dicarbonate (543 mg, 2.49 mmol) and 200 mg of 10% palladium carbon were added, and the mixture was reacted at room temperature for 6 hours under the protection of hydrogen. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to obtain a crude product of ethyl 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 8f. The product was used in the next reaction without purification. MS m / z(ESI):514.2[M+1] (Step 6) 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid The crude product of 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 8f (1.06 g) was dissolved in 5 mL of a mixture (tetrahydrofuran:ethanol:water = 2:2:1), lithium hydroxide monohydrate (868 mg, 20.7 mmol) was added, and the mixture was stirred at 80 ° C. overnight. After the completion of the reaction was monitored by LC-MS, the reaction solution was cooled and diluted hydrochloric acid was added dropwise to adjust it to acidity, and then 20 mL of water was added and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 8 g of crude 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid, which was used in the next reaction without purification. MS m / z(ESI):486.2[M+1] (Step 7) 5-(((1R)-1-((2R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-((2R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 8g (1.00g) was added to 10mL of dichloromethane solution. Then, 10mL of hydrogen chloride·1,4-dioxane solution was added and reacted at room temperature for 1 hour. After monitoring the completion of the reaction by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-((2R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 8h. The product was used in the next reaction without purification. MS m / z(ESI):386.1[M+1] (Step 8) (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-((2R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 8h (797 mg), pentafluorophenyl diphenylphosphinate (1.08 g, 2.48 mmol) and N,N-diisopropylethylamine (2.14 g, 16.56 mmol) were dissolved in 12 mL of a mixture of dichloromethane: N,N-dimethylformamide = 5:1) and reacted at room temperature overnight. After the completion of the reaction was monitored by LC-MS, 30 mL of saturated aqueous ammonium chloride was added to the reaction solution and extracted with dichloromethane (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, concentrated under reduced pressure, and the liquid phase was prepared (separation column: AKZONOBEL Kromasil; 250 x 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) to obtain the title product, (3R,11R)-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 8 (200 mg) was obtained. MS m / z(ESI):368.1[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.62 (d, J = 4.9 Hz, 1H), 8.53 (d, J = 7.7 Hz, 1H), 8.00 (s, 1H), 6.80 (d, J = 7.9 Hz, 1H), 6.73 (d, J = 10.1 Hz, 1H), 6.37 (d, J = 7.6 Hz, 1H), 4.94 (s, 1H), 4.03 (d, J = 7.1 Hz, 1H), 3.79 (d, J = 9.5 Hz, 2H), 3.24-3.15 (m, 2H), 1.59 (s, 3H), 1.54 (d, J = 7.0 Hz, 3H). Example 9 (3R,11S)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0173] [ka]

[0174] [ka]

[0175] (Step 1) (R)-N-((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((1E)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 8b (1.02 g, 2.90 mmol) was added to 10 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (11.6 mL, 5.80 mmol, 0.5 mol / L) was added and reacted at room temperature for 4 hours. After completion of the reaction, the reaction solution was quenched with 30 mL of water and extracted with ethyl acetate (30 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was further separated and purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 9a (793 mg) in a yield of 77.5%. MS m / z(ESI):355.1[M+1] (Step 2) (1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 9a (793 mg, 2.24 mmol) was added to 3 mL of dichloromethane solution. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of (1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 9b. The product was used in the next reaction without purification. MS m / z(ESI):234.1[M-16] (Step 3) 5-(((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl The crude product of (1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 9b (560 mg), ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (504 mg, 2.24 mmol) and N,N-diisopropylethylamine (2.32 g, 17.92 mmol) were dissolved in 10 mL of n-butanol and reacted at 125° C. for 4 hours. After the completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure, and the resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to give ethyl 5-(((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 9c (610 mg, pale yellow solid) in 61.9% yield. MS m / z(ESI):440.0[M+1] (Step 4) 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-((2S)-2-(azidomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 9c (610 mg, 1.39 mmol) was added to 10 mL of methanol. Then, di-tert-butyl dicarbonate (364 mg, 1.67 mmol) and 200 g of wet palladium carbon were added, and the mixture was reacted at room temperature for 6 hours under the protection of hydrogen. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to obtain a crude product of ethyl 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 9d. The product was used in the next reaction without purification. MS m / z(ESI):514.2[M+1] (Step 5) 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid The crude product of 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 9d (713 mg) was dissolved in 5 mL of a mixture solution (tetrahydrofuran:ethanol:water = 2:2:1), lithium hydroxide monohydrate (583 mg, 13.9 mmol) was added, and the mixture was stirred at 80 ° C. overnight. After the completion of the reaction was monitored by LC-MS, the reaction solution was cooled and diluted hydrochloric acid was added dropwise to adjust it to acidity, and then 20 mL of water was added and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 9e, which was used in the next reaction without purification. MS m / z(ESI):486.2[M+1] (Step 6) 5-(((1R)-1-((2S)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-((2S)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 9e (674 mg) was added to 8 mL of dichloromethane solution. Then, 8 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction was monitored by LC-MS, the reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-((2S)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 9f. The product was used in the next reaction without purification. MS m / z(ESI):386.1[M+1] (Step 7) (3R,11S)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-((2S)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 9f (535 mg), pentafluorophenyl diphenylphosphinate (641 mg, 1.67 mmol) and N,N-diisopropylethylamine (1.44 g, 11.12 mmol) were dissolved in 6 mL of a mixture (dichloromethane: N-N-dimethylformamide = 5: 1) and reacted at room temperature overnight. After the completion of the reaction was monitored by LC-MS, 20 mL of saturated aqueous ammonium chloride was added to the reaction solution and extracted with dichloromethane (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to prepare a liquid phase (separation column: AKZONOBEL Kromasil; 250×21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA+H2O, mobile phase B: CH3CN) to obtain the title product, (3R,11S)-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 9 (90 mg) was obtained. MS m / z(ESI):368.1[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.64 (d, J = 5.0 Hz, 1H), 8.37 (d, J = 8.0 Hz, 1H), 8.10 (s, 1H), 6.8 0(d, J = 7.9 Hz, 1H), 6.74 (d, J = 10.0 Hz, 1H), 6.37 (d, J = 7.6 Hz, 1H), 4.93 (s, 1H), 4.03 (d, J = 7.1 Hz, 1H), 3.93-3.68(m, 2H), 3.66-3.58 (m, 2H), 1.60 (m, 3H), 1.53 (d, J = 7.0 Hz, 3H). [Examples 10 and 11] (3R,11R)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 10 (3R,11S)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 11

[0176] [ka]

[0177] [ka]

[0178] (Step 1) 1-(4,5-difluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 1-(4,5-difluoro-2-hydroxyphenyl)ethan-1-one 10a (5.8 g, 33.7 mmol) was dissolved in 30 mL of N,N-dimethylformamide. Then, 3-bromo-2-methylpropene (4.5 g, 33.7 mmol) and potassium carbonate (9.3 g, 67.4 mmol) were added in sequence and reacted at room temperature for 4 hours. After the reaction was completed, 200 mL of water was added and extracted with ethyl acetate (100 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (300 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 1-(4,5-difluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 10b (7.2 g, gray liquid) in 94.5% yield. MS m / z(ESI):227.1[M+1] (Step 2) 1-(2,3-Difluoro-6-hydroxy-5-(2-methylallyl)phenyl)ethan-1-one 10c 1-(4,5-Difluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 10d 1-(4,5-difluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 10b (7.2 g, 31.8 mmol) was heated to 220 ° C., stirred and reacted for 10 hours. After completion of the reaction, the reaction solution was cooled to room temperature, dissolved in 100 mL of dichloromethane, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(2,3-difluoro-6-hydroxy-5-(2-methylallyl)phenyl)ethan-1-one 10c (700 mg, yellow liquid, yield 9.7%) and 1-(4,5-difluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 10d (2.7 g, yellow liquid, yield 37.6%). 10c MS m / z(ESI):227.1[M+1] 10d MS m / z(ESI):227.1[M+1] (Step 3) 1-(5,6-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(2,3-difluoro-6-hydroxy-5-(2-methylallyl)phenyl)ethan-1-one 10c (700 mg, 3.08 mmol) was dissolved in 10 mL of tetrahydrofuran, and N-iodosuccinimide (1.39 g, 6.16 mmol) was added and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution, and then sodium thiosulfate solids were gradually added and dissolved by stirring until the color of the solution was no longer bright, and extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5,6-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 10e (440 mg, yellow viscous substance) in a yield of 40.7%. MS m / z(ESI):353.2[M+1] (Step 4) 1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5,6-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 10e (440 mg, 1.25 mmol) was dissolved in 3 mL of N,N-dimethylformamide. Sodium azide (98 mg, 1.5 mmol) was then added, and the mixture was heated to 60° C. and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature, 100 mL of water was added, and the mixture was extracted with ethyl acetate (50 mL×3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 10f (300 mg, light brown viscous substance) in a yield of 90.9%. MS m / z(ESI):268.0[M+1] (Step 5) (R)-N-((E)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 10f (300 mg, 1.12 mmol) was dissolved in 5 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (272 mg, 2.25 mmol) and tetraethyl titanate (1.02 g, 4.48 mmol) were added in sequence, and the mixture was refluxed and reacted for 4 hours. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 10g (370 mg, yellow viscous substance) in a yield of 89.16%. MS m / z(ESI):371.2[M+1] (Step 6) (R)-N-((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 10g (370 mg, 1.0 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (4 mL, 2.0 mmol, 0.5 mol / L) was added and reacted at room temperature for 2 hours. After the reaction was completed, the reaction solution was quenched with 30 mL of methanol and concentrated under reduced pressure to obtain the crude product of (R)-N-((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 10h (372 mg, yellow viscous material). The product was used directly in the next reaction. MS m / z(ESI):373.2[M+1] (Step 7) (1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 10h (372 mg, 1.0 mmol) was dissolved in 5 mL of dichloromethane. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After the reaction was completed, the reaction solution was concentrated under reduced pressure to obtain the crude product (1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 10i (268 mg). The product was used directly in the next reaction without purification. MS m / z(ESI):268.2[M+1] (Step 8) 5-(((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 10i (268 mg, 1.0 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (225 mg, 1.0 mmol) were dissolved in 3 mL of n-butanol, and N,N-diisopropylethylamine (1.03 g, 8.0 mmol) was added, heated to 125° C., and reacted for 6 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 10j (120 mg, yellow viscous substance) in a yield of 26.2%. MS m / z(ESI):458.2[M+1] (Step 9) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 10j (120 mg, 0.26 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (338 mg, 1.55 mmol) and 10% palladium carbon (30 mg, containing 30% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature for 5 hours. After completion of the reaction, the reaction solution was filtered through diatomaceous earth, and the filtrate was concentrated under reduced pressure to obtain crude product of ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 10k (138 mg, yellow viscous material). The product was used directly in the next reaction without purification. MS m / z(ESI):532.2[M+1] (Step 10) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 10k (138 mg, 0.26 mmol) was dissolved in 4 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 2:1:1), lithium hydroxide monohydrate (110 mg, 2.6 mmol) was added, heated to 80 ° C., and reacted overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to remove ethanol and tetrahydrofuran, 20 mL of water was added, and the mixture was adjusted to acidity with 1.0 M dilute hydrochloric acid, and then extracted with ethyl acetate (15 mL × 3). The organic phases were then combined, washed with 50 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 10l (123 mg, yellow gum). The product was used directly in the next reaction without further purification. MS m / z(ESI):504.2[M+1] (Step 11) 5-(((1R)-1-(2-(aminomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 10l (123mg, 0.24mmol) was dissolved in 2mL of dichloromethane. Then, 4mL of hydrogen chloride·1,4-dioxane solution (4mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain a crude product (98mg) of 5-(((1R)-1-(2-(aminomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 10m. The product was used directly in the next reaction without purification. MS m / z(ESI):404.2[M+1] (Step 12) (3R,11R)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 10 (3R,11S)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 11 5-(((1R)-1-(2-(aminomethyl)-5,6-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 10m (98 mg, 0.24 mmol), pentafluorophenyl diphenylphosphinate (111 mg, 0.28 mmol) and N,N-diisopropylethylamine (248 mg, 1.92 mmol) were dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=5:1) and reacted at room temperature overnight. After completion of the reaction, 20 mL of saturated aqueous ammonium chloride solution was added to the reaction solution and extracted with dichloromethane (20 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11R)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 10 (20 mg, 20.4% yield) and (3R,11S)-5,6-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 11 (40 mg, 40.8% yield) was obtained. 10 MS m / z(ESI):386.2[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.97 (d, J = 6.7 Hz, 1H), 8.86 (d, J = 9.7 Hz, 1H), 8.49 (d, J = 7.4 Hz, 1H), 8.00 (d, J = 5.9 Hz, 1H), 7.31 (s, 1H), 7.08 (d, J = 8.7 Hz, 1H), 4.98-4.89 (m, 1H), 3.83 (dd, J = 16.3, 7.3 Hz, 1H), 3.16-3.15 (m, 1H), 3.03-3.01 (m, 1H), 2.98-2.96 (m, 1H), 1.82 (s, 3H), 1.56 (d, J = 18.1 Hz, 3H). 11 MS m / z(ESI):386.2[M+1] 1 H NMR (400 MHz, DMSO-d6) δ 8.92 (d, J = 9.5 Hz, 1H), 8.75 (d, J = 5.2 Hz, 1H), 8.55 (d, J = 7.6 Hz, 1H), 8.01 (s, 1H), 7.06 (t, J = 8.9 Hz, 1H), 6.41 (d, J = 7.6 Hz, 1H), 5.01 (t, J = 6.7 Hz, 1H), 3.81 (dd, J = 13.3, 9.8 Hz, 1H), 3.27-3.15 (m, 2H), 3.00 (d, J = 16.6 Hz, 1H), 1.67 (d, J = 7.2 Hz, 3H), 1.59 (s, 3H). [Example 12] (3R)-6,7-ジフルオロ-3,11-ジメチル-10-オキサ-2,13,17,18,21-ペンタアザペンタシクロ[13.5.2.1 8,11 .0 4,9 .0 18,22 ]トリコサ-1(21),4,6,8,15(22),16,19-ヘプタエン-14-オン

[0179]

change

[0180] [ka]

[0181] (Step 1) 1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(4,5-difluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 10d (2.7 g, 11.9 mmol) was dissolved in 50 mL of tetrahydrofuran, N-iodosuccinimide (5.4 g, 23.8 mmol) was added, and the mixture was allowed to react at room temperature overnight. After completion of the reaction, sodium thiosulfate was added to the reaction solution to decolorize it, and then 50 mL of water was added and extracted with ethyl acetate (100 mL x 3). The organic phases were then combined, washed with 300 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (elution system: A) to obtain 1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 12a (1.35 g, white solid) in a yield of 37.5%. MS m / z(ESI):353.0[M+1] (Step 2) (R)-N-((E)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 12a (352 mg, 1.0 mmol) was dissolved in 5 mL of tetrahydrofuran, and (R)-2-methylpropane-2-sulfinamide 1b (242 mg, 2.0 mmol) and tetraethyl titanate (912 g, 4.0 mmol) were added in sequence and reacted at 75° C. for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature, 20 mL of water was added, and the mixture was filtered. The filtrate was extracted with ethyl acetate (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 12b (162 mg, yellow oil) in a yield of 36%. MS m / z(ESI):456.0[M+1] (Step 3) (R)-N-((1R)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 12b (162 mg, 0.36 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (1.4 mL, 0.72 mmol, 0.5 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 30 mL of methanol and concentrated under reduced pressure to give (R)-N-((1R)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 12c (130 mg, yellow oil) in 78% yield. MS m / z(ESI):458.0[M+1] (Step 4) (R)-N-((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((1R)-1-(4,5-difluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 12c (1.65 g, 3.6 mmol) was dissolved in 20 mL of N,N-dimethylformamide, sodium azide (351 mg, 5.4 mmol) was added, and the mixture was heated to 95° C. and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature, 50 mL of water was added, and the mixture was extracted with ethyl acetate (50 mL×3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 12d (700 mg, yellow oily substance) in a yield of 52%. MS m / z(ESI):373.1[M+1] (Step 5) (1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 12d (700 mg, 1.9 mmol) was dissolved in 5 mL of dichloromethane. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain the crude product (1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 12e (500 mg). The product was used directly in the next reaction without purification. MS m / z(ESI):269.1[M+1] (Step 6) 5-(((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 12e (500 mg, 1.87 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (420.75 mg, 1.87 mmol) was dissolved in 10 mL of n-butanol, N,N-diisopropylethylamine (1.45 g, 11.22 mmol) was added, and the mixture was heated to 125° C. and reacted for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain 5-(((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 12f (550 mg, yellow oily substance) in a yield of 64%. MS m / z(ESI):458.0[M+1] (Step 7) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 12f (550 mg, 1.2 mmol) was dissolved in 10 mL of methanol. Then, di-tert-butyl dicarbonate (315 mg, 1.2 mmol) and 10% palladium carbon (100 mg, containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature for 4 hours. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to obtain crude product 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 12g (637 mg, yellow oily substance). The product was used directly in the next reaction without purification. MS m / z(ESI):532.1[M+1] (Step 8) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 12g (637mg, 1.2mmol) of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl was dissolved in 12mL of a mixed solvent (V:V:V=5:5:2) of ethanol, tetrahydrofuran and water, lithium hydroxide monohydrate (503.5mg, 12mmol) was added, and the mixture was heated to 80°C and reacted overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to remove ethanol and tetrahydrofuran. Then, 50mL of water was added, and the mixture was acidified with 2.0M diluted hydrochloric acid, followed by extraction with ethyl acetate (20mL×3). The organic phases were then combined, washed with 50 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 12h (603 mg, yellow solid) in 99% yield. MS m / z(ESI):504.2[M+1] (Step 9) 5-(((1R)-1-(2-(aminomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 12h (603 mg, 1.2 mmol) was dissolved in 5 mL of dichloromethane. Then, 5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After the reaction was completed, the reaction was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 12i (484 mg, yellow oily substance). The product was used directly in the next reaction without purification. MS m / z(ESI):404.2[M+1] (Step 10) (3R)-6,7-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-4,5-difluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 12i (484 mg, 1.2 mmol), pentafluorophenyl diphenylphosphinate (554 mg, 1.44 mmol) and N,N-diisopropylethylamine (1.24 g, 9.6 mmol) were dissolved in 10 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=1:1) and reacted at room temperature overnight. After completion of the reaction, 20 mL of saturated aqueous ammonium chloride solution was added to the reaction solution and extracted with dichloromethane (20 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6,7-difluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 12 (20 mg) was obtained in 5% yield. MS m / z(ESI):386.1[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.88 (d, J = 9.5 Hz, 1H), 8.60 (d, J = 4.7 Hz, 1H), 8.58-8.52 (m, 1H), 8.01 (d, J = 2.3 Hz, 1H), 6.98 (dd, J = 11.5, 8.1 Hz, 1H), 6.38 (d, J = 7.8 Hz, 1H), 4.89 (t, J = 6.4 Hz, 1H), 3.84 (dd, J = 13.4, 9.8 Hz, 1H), 3.56 (dd, J = 14.2, 7.2 Hz, 1H), 3.25 (s, 1H), 3.10 (d, J = 16.8 Hz, 1H), 1. 62 (s, 3H), 1.53 (d, J = 7.3 Hz, 3H). Example 13 (3R)-7-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0182] [ka]

[0183] [ka]

[0184] (Step 1) 1-(4-fluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 1-(4-fluoro-2-hydroxyphenyl)ethan-1-one 13a (1.54 g, 10 mmol) was dissolved in 30 ml of N,N-dimethylformamide. Then, 3-bromo-2-methylpropene (1.62 g, 12 mmol) and potassium carbonate (2.76 g, 20 mmol) were added in sequence and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution and extracted with ethyl acetate (50 mL x 2). The organic phases were then combined, washed with saturated sodium chloride solution (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 1-(4-fluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 13b (2.08 g, white solid). The product was used directly in the next reaction without further purification. MS m / z(ESI):209.0[M+1] (Step 2) 1-(4-fluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one The crude product (2.08 g) of 1-(4-fluoro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 13b was heated to 220° C. and reacted with stirring for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature, dissolved in 100 mL of dichloromethane, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain 1-(4-fluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 13c (1.35 g, yellow oily substance) in a yield of 65%. MS m / z(ESI):209.0[M+1] (Step 3) 1-(4-Fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(4-fluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 13c (5.4 g, 25.96 mmol) was dissolved in 50 mL of tetrahydrofuran, and N-iodosuccinimide (11.7 g, 51.92 mmol) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure and diluted by adding 50 mL of ethyl acetate. It was then washed with saturated sodium bisulfite solution (30 mL x 2) and saturated sodium chloride solution (20 mL) successively, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 13d (3.4 g, white solid) in a yield of 39%. MS m / z(ESI):335.0[M+1] (Step 4) (R)-N-((E)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(4-Fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 13d (2.98 g, 8.92 mmol) was dissolved in 40 mL of tetrahydrofuran and (R)-2-methylpropane-2-sulfinamide 1b (2.16 g, 17.8 mmol) and tetraethyl titanate (8.14 g, 35.68 mmol) were added in sequence, and then the mixture was refluxed overnight to react. After completion of the reaction, the reaction solution was cooled to room temperature, and 40 mL of ethyl acetate and 50 mL of water were added, followed by filtration. The filtrate was separated, the organic phase was retained, and the aqueous phase was extracted with ethyl acetate (30 mL x 2). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (elution system: A) to give (R)-N-((E)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 13e (980 mg, yellow oily substance) in 30% yield. MS m / z(ESI):438.0[M+1] (Step 5) (R)-N-((1R)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 13e (980 mg, 2.24 mmol) was dissolved in 10 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (9 mL, 4.48 mmol, 0.5 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 30 mL of methanol and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((1R)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 13f (770 mg, white solid) in a yield of 78.3%. MS m / z(ESI):440.0[M+1] (Step 6) (R)-N-((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((1R)-1-(4-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 13f (770 mg, 1.75 mmol) was dissolved in 10 mL of N,N-dimethylformamide, sodium azide (228 mg, 3.5 mmol) was added, and the mixture was reacted at 95 ° C. overnight. After the reaction was completed, the reaction solution was cooled to room temperature, 20 mL of water was added, and the mixture was extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, washed with water (10 mL) and saturated sodium chloride solution (100 mL × 2) in sequence, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain 13g (450mg, colorless oily substance) of (R)-N-((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide in a yield of 72.7%. MS m / z(ESI):355.1[M+1] (Step 7) (1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 13g (450 mg, 1.27 mmol) was dissolved in 4 mL of dichloromethane. Then, 1 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain the crude product (1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 13h (317 mg, colorless liquid). The product was used directly in the next reaction without purification. MS M / z(ESI):234.0[M-16] (Step 8) 5-(((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 13h (317 mg, 1.27 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (343 mg, 1.52 mmol) were dissolved in 10 mL of n-butanol. Then, N,N-diisopropylethylamine (1.31 g, 10.16 mmol) was added, heated to 125 ° C, and reacted for 4 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 13i (380 mg, white solid) in a yield of 68%. MS m / z(ESI):440.0[M+1] (Step 9) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 13i (380 mg, 0.86 mmol) was dissolved in 10 mL of methanol. Then, di-tert-butyl dicarbonate (226 mg, 1.04 mmol) and 10% palladium carbon (80 mg, containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature overnight. After the reaction was completed, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 13j (200 mg, white solid) in a yield of 45%. MS m / z(ESI):514.0[M+1] (Step 10) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 13j (200 mg, 0.39 mmol) was dissolved in 5 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V=2:2:1), lithium hydroxide monohydrate (130 mg, 3.1 mmol) was added, and the mixture was heated to 85° C. and reacted for 4 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure to remove ethanol and tetrahydrofuran, diluted with 20 mL of ethyl acetate, washed successively with 10% aqueous citric acid solution (20 mL) and saturated sodium chloride solution (20 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 13k (189 mg, white solid). The product was used directly in the next reaction. MS m / z(ESI):386.0[M-100] (Step 11) 5-(((1R)-1-(2-(aminomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 13k (189 mg, 0.39 mmol) was dissolved in 2 mL of dichloromethane. Then, 0.5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After the reaction was completed, the reaction was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 13l (150 mg, red oily substance). The product was used directly in the next reaction without purification. MS m / z(ESI):386.0[M+1] (Step 12) (3R)-7-Fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-4-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 13l (150 mg, 0.26 mmol), pentafluorophenyl diphenylphosphinate (119.8 mg, 0.31 mmol) and N,N-diisopropylethylamine (268 mg, 2.08 mmol) were dissolved in 4 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=1:1) and reacted at room temperature for 4 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure, dissolved in 10 mL of ethyl acetate, washed with saturated sodium chloride solution (10 mL x 2), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-7-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21), 4, 6, 8, 15 (22), 16, 19-heptaen-14-one 13 (20 mg) was obtained in a yield of 21.1%. MS m / z (ESI): 368.0 [M+1] 1H NMR (400 MHz, CDCl3) δ 9.22-9.20 (d, J = 8.0 Hz, 1H), 8.22-8.14 (m, 2H), 6.97-6.94 (m, 1H), 6.49-6.44 (m, 1H), 6.14-6.12 (m, 1H), 5.91 (s, 1H), 5.19-5.13 (m, 1H), 4.02-3.96 (m, 1H), 3.43-3.23 (m, 3H), 1.67-1.64 (m, 6H). [Examples 14 and 15] (3R,11S)-6-Fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .018,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 14 (3R,11R)-6-Fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 15

[0185] [ka]

[0186] [ka]

[0187] (Step 1) 1-(2-((2-(bromomethyl)allyl)oxy)-5-fluorophenyl)ethan-1-one 3-Bromo-2-(bromomethyl)prop-1-ene 14b (4.28 g, 20 mmol) and potassium carbonate (1.66 g, 12 mmol) were dissolved in 40 mL of N,N-dimethylformamide, and 1-(5-fluoro-2-hydroxyphenyl)ethan-1-one 14a (1.54 g, 10 mmol) was dissolved in 15 mL of N,N-dimethylformamide, added dropwise to the above mixture, and reacted at room temperature overnight. After completion of the reaction, 100 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (200 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(2-((2-(bromomethyl)allyl)oxy)-5-fluorophenyl)ethan-1-one 14c (1.8 g, gray liquid) in a yield of 62.5%. MS m / z(ESI):287.1[M+1] (Step 2) 1-(5-fluoro-2-((2-(methoxymethyl)allyl)oxy)phenyl)ethan-1-one 1-(2-((2-(bromomethyl)allyl)oxy)-5-fluorophenyl)ethan-1-one 14c (1.8 g, 6.29 mmol) was dissolved in 20 mL of methanol, sodium methanolate (1.37 g, 25.16 mmol) was added, and the mixture was reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure, dissolved by adding 100 mL of water, and extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (200 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(5-fluoro-2-((2-(methoxymethyl)allyl)oxy)phenyl)ethan-1-one 14d (1.1 g, yellow oily substance) in a yield of 73.8%. MS m / z(ESI):239.1[M+1] (Step 3) 1-(5-fluoro-2-hydroxy-3-(2-(methoxymethyl)allyl)phenyl)ethan-1-one 1-(5-fluoro-2-((2-(methoxymethyl)allyl)oxy)phenyl)ethan-1-one 14d (1.1 g, 4.6 mmol) was heated to 220° C. and stirred for 8 hours. After completion of the reaction, the reaction solution was cooled to room temperature to obtain the crude product 1-(5-fluoro-2-hydroxy-3-(2-(methoxymethyl)allyl)phenyl)ethan-1-one 14e (1.1 g, brown viscous material). The product was used directly in the next reaction without purification. MS m / z(ESI):239.1[M+1] (Step 4) 1-(5-Fluoro-2-(iodomethyl)-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Fluoro-2-hydroxy-3-(2-(methoxymethyl)allyl)phenyl)ethan-1-one 14e (1.1 g, 4.6 mmol) was dissolved in 10 mL of acetonitrile, and iodine (2.34 g, 9.2 mmol) and sodium bicarbonate (1.55 g, 18.4 mmol) were added and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution, and solid sodium thiosulfate was gradually added and dissolved by stirring until the color of the solution was no longer bright, and extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5-fluoro-2-(iodomethyl)-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 14f (1.4 g, brown viscous substance) in a yield of 83.6%. MS m / z(ESI):365.0[M+1] (Step 5) 1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Fluoro-2-(iodomethyl)-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 14f (1.4 g, 3.84 mmol) was dissolved in 15 mL of N,N-dimethylformamide, sodium azide (500 mg, 7.69 mmol) was added, and the mixture was heated to 60° C. and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature, poured into 100 mL of water, and extracted with ethyl acetate (50 mL×3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 14g (430 mg, yellow viscous substance) in a yield of 41.2%. MS m / z(ESI):280.1[M+1] (Step 6) (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 14g (430mg, 1.54mmol) was dissolved in 10mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (373mg, 3.08mmol) and tetraethyl titanate (1.41g, 6.16mmol) were added in sequence, and the mixture was refluxed and reacted for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 14h (440 mg, yellow viscous substance) in a yield of 74.8%. MS m / z(ESI):383.2[M+1] (Step 7) (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 14h (440 mg, 1.15 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (4.6 mL, 2.31 mmol, 0.5 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 30 mL of methanol and concentrated under reduced pressure to obtain the crude product of (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 14i (440 mg, yellow viscous material). The product was used directly in the next reaction. MS m / z(ESI):385.2[M+1] (Step 8) (1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 14i (440 mg, 1.15 mmol) was dissolved in 5 mL of dichloromethane. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain the crude product of (1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 14j (320 mg, yellow viscous material). The product was used directly in the next reaction without purification. MS m / z(ESI):281.1[M+1] (Step 9) 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 14j (320 mg, 1.14 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (257 mg, 1.14 mmol) were dissolved in 5 mL of n-butanol, N,N-diisopropylethylamine (1.18 g, 9.12 mmol) was added, and the mixture was heated to 135° C. and reacted for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 14k (170 mg, yellow viscous substance) in a yield of 31.8%. MS m / z(ESI):470.3[M+1] (Step 10) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 14k (170 mg, 0.36 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (97 mg, 0.44 mmol) and 10% palladium carbon (50 mg, containing 50% water) were added, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was allowed to proceed at room temperature overnight. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to obtain crude product of ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 14l (197 mg, yellow viscous substance). The product was used directly in the next reaction without purification. MS m / z(ESI):544.3[M+1] (Step 11) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 14l (197mg, 0.36mmol) was dissolved in 7mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V=5:1:1), lithium hydroxide monohydrate (152mg, 3.6mmol) was added, heated to 90°C, and reacted for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure to remove ethanol and tetrahydrofuran. Then, 20mL of water was added, and the mixture was acidified with 1.0M diluted hydrochloric acid, and extracted with ethyl acetate (15mL×3). The organic phases were then combined, washed with 50 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 14m (187 mg, yellow foamy solid), which was used directly in the next reaction without purification. MS m / z(ESI):516.2[M+1] (Step 12) 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 14m (187 mg, 0.36 mmol) was dissolved in 1 mL of dichloromethane. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After the reaction was completed, the reaction was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 14n (151 mg). The product was used directly in the next reaction without purification. MS m / z(ESI):416.2[M+1] (Step 13) (3R,11S)-6-Fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 14 (3R,11R)-6-Fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 15 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(methoxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 14n (151 mg, 0.36 mmol), pentafluorophenyl diphenylphosphinate (167 mg, 0.44 mmol) and N,N-diisopropylethylamine (374 mg, 2.93 mmol) were dissolved in 4 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=3:1) and reacted at room temperature overnight. After completion of the reaction, 50 mL of saturated aqueous ammonium chloride solution was added to the reaction solution and extracted with dichloromethane (30 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 50 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11S)-6-fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 14 (3.85 mg, 2.7% yield) and (3R,11R)-6-fluoro-11-(methoxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] to obtain tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 15 (30 mg, 21% yield). 14 MS m / z(ESI):398.2[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.66 (d, J = 7.6 Hz, 1H), 8.37 (d, J = 8.0 Hz, 1H), 8.09 (s, 1H), 7.94 (d, J = 9.5 Hz, 1H), 7.03 (d, J = 8.0 Hz, 1H), 6.66 (d, J = 10.4 Hz, 1H), 6.60 (d, J = 7.5 Hz, 1H), 5.48 (t, J = 7.3 Hz, 1H), 3.76-3.60 (m, 1H), 3.14 (d, J = 14.0 Hz, 2H), 3.03 (s, 1H), 2.99 (s, 1H), 2.92 (s, 3H), 2.73 (s, 1H), 1.56 (d, J = 6.9 Hz, 3H). 15 MS m / z(ESI):398.2[M+1] 1 H NMR (400 MHz, DMSO-d6) δ 8.89 (d, J = 9.3 Hz, 1H), 8.62 (d, J = 4.9 Hz, 1H), 8.54 (d, J = 7.6 Hz, 1H), 8.00 (s, 1H), 6.82 (dd, J = 8.2, 2.6 Hz, 1H), 6.74 (dd, J = 10.1, 2.7 Hz, 1H), 6.37 (d, J = 7.6 Hz, 1H), 4.95 (t, J = 6.8 Hz, 1H), 3. 78-3.68 (m, 1H), 3.67-3.58 (m, 2H), 3.46 (s, 1H), 3.36 (s, 3H), 3.28 (d, J = 13.1 Hz, 1H), 2.89 (d, J = 16.8 Hz, 1H), 1.54 (d, J = 7.0 Hz, 3H). [Example 16] (3R)-6-ブロモ-3,11-ジメチル-10-オキサ-2,13,17,18,21-ペンタアザペンタシクロ[13.5.2.1 8,11 .0 4,9 .0 18,22 ]トリコサ-1(21),4,6,8,15(22),16,19-ヘプタエン-14-オン

[0188] [ka]

[0189] [ka]

[0190] (Step 1) 1-(5-bromo-2-((2-methylallyl)oxy)phenyl)ethan-1-one 1-(5-bromo-2-hydroxyphenyl)ethan-1-one 16a (10 g, 46.5 mmol) was dissolved in 100 ml of N,N-dimethylformamide. Then, 3-bromo-2-methylpropene (7.53 g, 55.8 mmol) and potassium carbonate (12.8 g, 93 mmol) were added in sequence and reacted at room temperature overnight. After the reaction was completed, 200 mL of water was added to the reaction solution and extracted with ethyl acetate (100 mL). The organic phases were then combined, washed with saturated sodium chloride solution (100 mL x 2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain the crude product of 1-(5-bromo-2-((2-methylallyl)oxy)phenyl)ethan-1-one 16b (12.46 g, yellow oily substance). The product was used directly in the next reaction without further purification. MS m / z(ESI):269.0[M+1] (Step 2) 1-(5-bromo-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 1-(5-bromo-2-((2-methylallyl)oxy)phenyl)ethan-1-one 16b (12.46 g, 46.5 mmol) was heated to 220° C. and reacted with stirring for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature, dissolved in 100 mL of dichloromethane, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain 1-(5-bromo-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 16c (6.97 g, red oily substance) in a yield of 56%. MS m / z(ESI):268.9[M+1] (Step 3) 1-(5-bromo-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Bromo-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 16c (11 g, 41 mmol) was dissolved in 200 mL of acetonitrile, and iodine (20.8 g, 82 mmol) and sodium bicarbonate (13.8 g, 164 mmol) were added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The residue was dissolved in 200 mL of ethyl acetate, washed with 25% saturated sodium bisulfite solution (100 mL x 2) and saturated sodium chloride solution (100 mL x 2) in sequence, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5-bromo-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 16d (15 g, white solid) in a yield of 92.6%. MS m / z(ESI):394.5[M+1] (Step 4) 1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Bromo-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 16d (6.4 g, 16.2 mmol) was dissolved in 50 mL of N,N-dimethylformamide, sodium azide (2.1 g, 32.4 mmol) was added, and the mixture was heated to 95° C. and reacted overnight. After the reaction was completed, the reaction solution was cooled to room temperature, and then 100 mL of water was added and extracted with ethyl acetate (50 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (50 mL×3), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 16e (3.97 g, white solid) in a yield of 79.4%. MS m / z(ESI):310.9[M+1] (Step 5) (R)-N-((E)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 16e (3.97 g, 12.8 mmol) was dissolved in 50 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (3.1 g, 25.6 mmol) and tetraethyl titanate (11.67 g, 51.2 mmol) were added in sequence, and the mixture was reacted at 75 ° C overnight. After the reaction was completed, the reaction solution was cooled to room temperature, and 100 mL of water was added to precipitate a large amount of solids, which were then filtered. The filter cake was rinsed with 100 mL of ethyl acetate to obtain the filtrate, which was then extracted with ethyl acetate (50 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give (R)-N-((E)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide. 16f (5.17 g, white solid) was obtained in 97.8% yield. MS m / z(ESI):413.0[M+1] (Step 6) (R)-N-((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 16f (5.17 g, 12.5 mmol) was dissolved in 100 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (50 mL, 25 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 100 mL of methanol and concentrated under reduced pressure to obtain (R)-N-((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 16g (4.5 g, white solid) in 86.7% yield. MS m / z(ESI):415.0[M+1] (Step 7) (1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 16g (4.5 g, 10.8 mmol) was dissolved in 40 mL of dichloromethane. Then, 10 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain (1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 16h (3.36 g, white solid) in 100% yield. MS m / z(ESI):294.0[M-16] (Step 8) 5-(((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 16h (3.36 g, 10.8 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (2.67 g, 11.8 mmol) were dissolved in 60 mL of n-butanol. Then, N,N-diisopropylethylamine (15 mL, 86.4 mmol) was added and the mixture was heated to 125° C. and reacted for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give ethyl 5-(((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate. 16i (4.5 g, white solid) was obtained in 83.3% yield. MS m / z(ESI):501.1[M+1] (Step 9) 5-(((1R)-1-(5-bromo-2-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 16i (3.19 mg, 6.38 mmol) was dissolved in 25 mL of methanol, triphenylphosphine (2.5 g, 9.57 mmol) was added, and the mixture was reacted at 80° C. for 1.5 hours. After the reaction was completed, the reaction solution was concentrated under reduced pressure, diluted with 50 mL of ethyl acetate, and adjusted to a pH value of 4 with 1.0 M dilute hydrochloric acid. The reaction solution was separated and the aqueous phase was collected, and the pH value was adjusted to 10 with 25% aqueous sodium hydroxide solution, and the reaction solution was extracted with ethyl acetate (100 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (100 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was dissolved in 100 mL of dichloromethane, and di-tert-butyl dicarbonate (2.09 g, 9.57 mmol) and triethylamine (1.3 g, 12.76 mmol) were added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(5-bromo-2-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 16j (2.776 g, white solid) in a yield of 76%. MS m / z(ESI): 473.9[M-100] (Step 10) 5-(((1R)-1-(5-bromo-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(5-bromo-2-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)ethyl pyrazolo[1,5-a]pyrimidine-3-carboxylate 16j (450 mg, 0.78 mmol) was dissolved in 5 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V=2:2:1), lithium hydroxide monohydrate (238 mg, 6.28 mmol) was added, and the mixture was heated to 80° C. and reacted for 2 hours. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure to remove ethanol and tetrahydrofuran. Then, 20 mL of water was added, the mixture was acidified with 1.0 M diluted hydrochloric acid, and extracted with ethyl acetate (20 mL×3). The organic phases were then combined, washed with 20 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give the crude product of 5-(((1R)-1-(5-bromo-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 16k (426 mg, white solid), which was used directly in the next reaction without purification. MS m / z(ESI):545.9[M+1] (Step 11) 5-(((1R)-1-(2-(aminomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(5-bromo-(((tert-butoxycarbonyl)amino)methyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 16k (426 mg, 0.78 mmol) was dissolved in 5 mL of dichloromethane. Then, 0.5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 2 hours. After the reaction was completed, the reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 16l (348 mg, white solid). The product was used directly in the next reaction without purification. MS m / z(ESI):445.9[M+1] (Step 12) (3R)-6-Bromo-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-bromo-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 16l (348 mg, 0.78 mmol), pentafluorophenyl diphenylphosphinate (360 mg, 0.94 mmol) and N,N-diisopropylethylamine (805 mg, 6.24 mmol) were dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=1:1) and reacted at room temperature overnight. After completion of the reaction, 10 mL of water was added to the reaction solution and extracted with ethyl acetate (10 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 10 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6-bromo-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 16 (100 mg) was obtained in 29.9% yield. MS m / z(ESI):427.9[M+1] 1 H NMR (400 MHz, CDCl3) δ 9.16-9.13 (d, J =12.0 Hz, 1H), 8.23-8.19 (m, 2H), 7.08 (s, 1H), 7.03 (s, 1H), 6.15-6.13 (d, J =8.0 Hz, 1H),5.72 (s, 1H), 5.19-5.15 (m, 1H), 3.99-3.94 (m, 1H), 3.41-3.15 (m, 3H), 1.66-1.65 (m, 6H). Example 17 (3R)-6-Cyano-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .04,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0191] [ka]

[0192] [ka]

[0193] (Step 1) (3R)-6-Cyano-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one (3R)-6-Bromo-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 16 (45 mg, 0.105 mmol), zinc cyanide (62 mg, 0.525 mmol), zinc powder (0.7 mg, 0.0105 mmol), 1,1'-bis(diphenylphosphino)ferrocene (29 mg, 0.053 mmol) and tris(dibenzylideneacetone)dipalladium (19.2 mg, 0.021 mmol) were dissolved in 5 mL of N,N-dimethylaniline and reacted at 130 °C for 3 h. After completion of the reaction, the reaction solution was cooled to room temperature, 10 mL of water was added, and extracted with ethyl acetate (10 mL × 3). The organic phases were then combined, washed with saturated sodium chloride solution (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6-cyano-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 17 (10 mg) was obtained in 25.6% yield. MS m / z(ESI):375.0[M+1] 1 H NMR (400 MHz,CDCl3) δ 9.02-9.00 (d, J = 8.0 Hz, 1H), 8.22-8.18 (m, 2H), 7.36 (s, 1H),7.21 (s, 1H), 6.19-6.17 (d, J =8.0 Hz, 1H),6.03 (s, 1H), 5.23-5.19 (m, 1H), 4.13-4.10 (m, 1H), 3.43-3.29 (m, 3H), 1.69-1.66 (m, 6H). Example 18 (3R)-6-Fluoro-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .018,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0194] [ka]

[0195] [ka]

[0196] (Step 1) 5-Methylene-1,3,2-dioxathiane-2-oxide 2-Methylenepropane-1,3-propanediol 18a was dissolved in 100 mL of carbon tetrachloride and cooled to 0°C. Thionyl chloride (50.7 g, 426 mmol) was dissolved in 50 mL of carbon tetrachloride and added dropwise to the above solution at 0°C, and reacted at 0°C for 1 hour. After completion of the reaction, the reaction solution was poured into 200 mL of ice water and extracted with dichloromethane (100 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (200 mL) and saturated sodium bicarbonate solution (200 mL) in succession, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 5-methylene-1,3,2-dioxathiane-2-oxide 18b (28.7 g, yellow liquid). The product was used directly in the next reaction. MS m / z(ESI): 135.0[M+1] (Step 2) 1-(5-fluoro-2-((2-(hydroxymethyl)allyl)oxy)phenyl)ethan-1-one 1-(5-fluoro-2-hydroxyphenyl)ethan-1-one 14a (33.0 g, 214 mmol) was dissolved in 100 mL of N,N-dimethylformamide, cooled to 0 ° C, sodium hydride (10.2 g, 256 mmol) was added gradually in batches, and stirred at 0 ° C for 0.5 h. Then, 5-methylene-1,3,2-dioxathiane-2-oxide 18b (28.7 g, 214 mmol) was added dropwise at 0 ° C, allowed to warm to room temperature naturally, heated to 50 ° C, reacted for 1 h, and then heated continuously to 100 ° C and reacted overnight. After the reaction was completed, the reaction solution was cooled to room temperature. The reaction solution was poured into 300 mL of ice water and extracted with ethyl acetate (200 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5-fluoro-2-((2-(hydroxymethyl)allyl)oxy)phenyl)ethan-1-one 18c (20 g, dark brown liquid) in a yield of 41.7%. MS m / z(ESI):225.2[M+1] (Step 3) 1-(5-fluoro-2-hydroxy-3-(2-(hydroxymethyl)allyl)phenyl)ethan-1-one 1-(5-fluoro-2-((2-(hydroxymethyl)allyl)oxy)phenyl)ethan-1-one 18c (20 g, 89.2 mmol) was heated to 220° C., stirred, and reacted for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature, dissolved in 100 mL of ethyl acetate, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain 1-(5-fluoro-2-hydroxy-3-(2-(hydroxymethyl)allyl)phenyl)ethan-1-one 18d (5.5 g, yellow liquid) in a yield of 27.5%. MS m / z(ESI):225.2[M+1] (Step 4) 1-(5-Fluoro-2-(hydroxymethyl)-2-(iodomethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Fluoro-2-hydroxy-3-(2-(hydroxymethyl)allyl)phenyl)ethan-1-one 18d (5.5 g, 24.55 mmol) was dissolved in 55 mL of acetonitrile, and iodine (12.4 g, 49.1 mmol) and sodium bicarbonate (8.24 g, 98.1 mmol) were added and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution, and it was extracted with ethyl acetate (100 mL x 3). Then, the organic phases were combined, washed with saturated sodium thiosulfate solution (200 mL x 3) until the color of the organic phase was no longer bright, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5-fluoro-2-(hydroxymethyl)-2-(iodomethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18e (5.4 g, yellow solid) in a yield of 62.8%. MS m / z (ESI): 351.1 [M+1] (Step 5) 1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-Fluoro-2-(hydroxymethyl)-2-(iodomethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18e (5.4 g, 15.4 mmol) was dissolved in 50 mL of N,N-dimethylformamide, sodium azide (2.0 g, 30.8 mmol) was added, and the mixture was heated to 85° C. and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature, 100 mL of water was added, and the mixture was extracted with ethyl acetate (50 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (100 mL×2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18f (2.9 g, brown viscous substance) in a yield of 70.7%. MS m / z(ESI):266.0[M+1] (Step 6) 1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18f (1 g, 3.77 mmol) was dissolved in 10 mL of dichloromethane, diethylaminosulfur trifluoride (728 mg, 4.5 mmol) was added, and the mixture was refluxed and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18g (440 mg, yellow liquid) in a yield of 44%. MS m / z(ESI):268.1[M+1] (Step 7) (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18g (440mg, 1.65mmol) was dissolved in 10mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (400mg, 3.3mmol) and tetraethyl titanate (1.51g, 6.6mmol) were added in sequence, and the mixture was refluxed at 90°C overnight. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 18h (420 mg, yellow viscous substance) in a yield of 68.9%. MS m / z(ESI):371.2[M+1] (Step 8) (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 18h (420 mg, 1.14 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (6.8 mL, 3.42 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction was quenched with 10 mL of methanol and concentrated under reduced pressure to give (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 18i (420 mg, yellow gum) in 100% yield. MS m / z(ESI):373.1[M+1] (Step 9) (1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 18i (420 mg, 1.13 mmol) was dissolved in 3 mL of dichloromethane. Then, 5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to give (1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 18j (303 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):269.2[M+1] (Step 10) 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 18j (303 mg, 1.13 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (255 mg, 1.13 mmol) were dissolved in 5 mL of n-butanol, and N,N-diisopropylethylamine (1.17 g, 9 mmol) was added, heated to 130° C., and reacted for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 18k (200 g, white solid) in a yield of 38.7%. MS m / z(ESI):458.3[M+1] (Step 11) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 18k (200 mg, 0.44 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (115 mg, 0.52 mmol) and 10% palladium carbon (containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature for 3 hours. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to give ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrazolo[1,5-a]pyrimidine-3-carboxylate 18l (232 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):532.2[M+1] (Step 12) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 18l (232 mg, 0.44 mmol) was dissolved in 5 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V=3:1:1), lithium hydroxide monohydrate (185 mg, 4.4 mmol) was added, heated to 95 ° C, and reacted for 2 hours. After the reaction was completed, the reaction solution was cooled to room temperature and concentrated under reduced pressure to remove ethanol and tetrahydrofuran. Then, 20 mL of water was added, the mixture was acidified with 1.0 M diluted hydrochloric acid, and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, washed with 20 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 18m (222 mg, yellow viscous material) in 100% yield. MS m / z(ESI):504.2[M+1] (Step 13) 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 18m (222 mg, 0.44 mmol) was dissolved in 5 mL of dichloromethane. Then, 4 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 0.5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain the crude product of 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 18n (178 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):404.3[M+1] (Step 14) (3R)-6-Fluoro-11-(fluoromethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(fluoromethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 18n (178 mg, 0.44 mmol), pentafluorophenyl diphenylphosphinate (203 mg, 0.88 mmol) and N,N-diisopropylethylamine (340.56 mg, 2.64 mmol) were dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=5:1) and reacted at room temperature overnight. After completion of the reaction, 10 mL of dichloromethane was added to the reaction solution, washed with saturated sodium chloride solution (20 mL x 3), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 10 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6-fluoro-11-(fluoromethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 18 (90 mg) was obtained in 53.2% yield. MS m / z(ESI):386.2[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.88 (d, J = 9.0 Hz, 1H), 8.65 (d, J = 4.8 Hz, 1H), 8.53 (dd, J = 7.6, 2.3 Hz, 1H), 8.00 (d, J = 2.4 Hz, 1H), 6.91-6.78 (m, 1H), 6. 76 (d, J = 2.7 Hz, 1H), 6.38 (dd, J = 7.6, 2.3 Hz, 1H), 4.96 (t, J = 6.5 Hz, 1H), 4.78 (q, J = 12.2, 11.4 Hz, 1H), 4.66 (q, J = 12.4, 11.5 Hz, 1H), 3.79 (dd, J = 13.0, 9.3 Hz, 1H), 3.40 (d, J = 16.4 Hz, 1H), 3.29 (s, 1H), 2.96 (d, J = 17.1 Hz, 1H), 1.55 (d, J = 6.8 Hz, 3H). Example 19 (3R)-6-Fluoro-11-(hydroxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0197] [ka]

[0198] [ka]

[0199] (Step 1) (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethan-1-one 18f (500 mg, 1.89 mmol) was dissolved in 5 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (459 mg, 3.79 mmol) and tetraethyl titanate (1.73 g, 7.59 mmol) were added in sequence, and the mixture was refluxed at 90° C. overnight. After completion of the reaction, the reaction solution was cooled to room temperature. The two reaction solutions were combined and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 19a (278 mg, yellow viscous substance) in an overall yield of 40%. MS m / z(ESI):369.1[M+1] (Step 2) (R)-N-((E)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 19a (50 mg, 0.136 mmol) was dissolved in 5 mL of N,N-dimethylformamide, and imidazole (19 mg, 0.28 mmol) and tert-butyldimethylsilyl chloride (24 mg, 0.16 mmol) were added and reacted at 75° C. for 6 hours. After completion of the reaction, the reaction solution was cooled to room temperature, poured into 100 mL of water, and extracted with ethyl acetate (20 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (100 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give (R)-N-((E)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 19b (50 mg, yellow gum) in 76.9% yield. MS m / z(ESI):483.3[M+1] (Step 3) (R)-N-((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 19b (50 mg, 0.1037 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (0.4 mL, 0.2 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction was quenched with 10 mL of methanol and concentrated under reduced pressure to give (R)-N-((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 19c (50 mg, yellow gum) in 100% yield. MS m / z(ESI):485.2[M+1] (Step 4) (1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 19c (1 g, 2.1 mmol) was dissolved in 11 mL of a mixed solvent of tetrahydrofuran and water (V:V=10:1), iodine (105 mg, 0.42 mmol) was added, and the mixture was reacted at 50 °C for 3 hours. After the reaction was completed, the mixture was cooled to room temperature, 50 mL of water was added, and then sodium thiosulfate solids were gradually added, stirred until the color of the solution was no longer bright, and dissolved, and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give (1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 19d (yellow gum), which was used directly in the next reaction without further purification. MS m / z(ESI):381.2[M+1] (Step 5) 5-(((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 19d and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (489 mg, 2.17 mmol) were dissolved in 5 mL of n-butanol, and N,N-diisopropylethylamine (2.25 g, 17.44 mmol) was added, heated to 130° C., and reacted for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give ethyl 5-(((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 19e (110 mg, white solid) in a two-step yield of 9.2%. MS m / z(ESI):570.3[M+1] (Step 6) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 19e (110 mg, 0.19 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (51 mg, 0.23 mmol) and 10% palladium carbon (50 mg, containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature for 3 hours. After completion of the reaction, the reaction solution was filtered and the filtrate was concentrated under reduced pressure to give ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 19f (122 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):644.3[M+1] (Step 7) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-2-(((tert-butyldimethylsilyl)oxy)methyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 19f (122 mg, 0.19 mmol) was dissolved in 7 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 5:1:1), lithium hydroxide monohydrate (160 mg, 3.8 mmol) was added, heated to 90 ° C, and reacted for 2 hours. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure to remove ethanol and tetrahydrofuran. Then, 20 mL of water was added, acidified with 1.0 M diluted hydrochloric acid, and extracted with ethyl acetate (20 mL × 3). The organic phases were then combined, washed with 50 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the crude product of 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 19g (95 mg, yellow gum) in 100% yield. MS m / z(ESI):502.2[M+1] (Step 8) 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 19g (95 mg, 0.19 mmol) was dissolved in 3 mL of dichloromethane. Then, 5 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 0.5 hours. After the reaction was completed, the reaction solution was concentrated under reduced pressure to obtain the crude product (76 mg) of 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 19h. The product was used directly in the next reaction without further purification. MS m / z(ESI):402.2[M+1] (Step 9) (3R)-6-Fluoro-11-(hydroxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-fluoro-2-(hydroxymethyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 19h (76 mg, 0.19 mmol), pentafluorophenyl diphenylphosphinate (88 mg, 0.228 mmol) and N,N-diisopropylethylamine (196 mg, 1.52 mmol) were dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=5:1) and reacted at room temperature overnight. After completion of the reaction, 10 mL of dichloromethane was added to the reaction solution, washed with water (20 mL x 3), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 10 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6-fluoro-11-(hydroxymethyl)-3-methyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 19 (14 mg) was obtained in 18.4% yield. MS m / z(ESI):384.1[M+1] Example 20 (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one

[0200] [ka]

[0201] [ka]

[0202] (Step 1) 1-(3-acetyl-5-fluoro-2-hydroxyphenyl)propan-2-one 1-(5-fluoro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 5a (5 g, 24 mmol) was dissolved in 500 mL of dichloromethane and stirred at room temperature. After introducing ozone, the resulting solution was reacted at room temperature for 6 hours. After completion of the reaction, the reaction solution was quenched with triphenylphosphine and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(3-acetyl-5-fluoro-2-hydroxyphenyl)propan-2-one 20a (2 g, yellow solid) in a yield of 39.7%. MS m / z(ESI):211.1[M+1] (Step 2) (E)-3-(2-(cyanomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)but-2-enenitrile Diethyl cyanomethylphosphonate (6.3 g, 35.5 mmol) was dissolved in 100 mL of tetrahydrofuran and cooled to 0° C., sodium hydride (1.42 g, 35.5 mmol) was added in batches slowly, and the mixture was reacted at 0° C. for 1 hour. Then, 1-(3-acetyl-5-fluoro-2-hydroxyphenyl)propan-2-one 20a (5 g, 23.7 mmol) was added, and the mixture was warmed to room temperature and reacted continuously overnight. After the reaction was completed, the system was poured into 50 mL of ice water and extracted with ethyl acetate (50 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain (E)-3-(2-(cyanomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)but-2-enenitrile 20b (1.1 g, yellow solid) in a yield of 18.1%. MS m / z(ESI):257.1[M+1] (Step 3) 2-(7-Acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile (E)-3-(2-(cyanomethyl)-5-fluoro-2,3-dihydrobenzofuran-7-yl)but-2-enenitrile 20b (1.9 g, 2.4 mmol) was dissolved in 50 mL of dichloromethane and stirred at room temperature. Ozone was introduced, and the resulting solution was reacted continuously at room temperature for 4 hours. After completion of the reaction, the reaction solution was quenched with triphenylphosphine and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 2-(7-acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 20c (560 mg, off-white solid) in 56% yield. MS m / z(ESI):234.1[M+1] (Step 4) (R)-N-((E)-1-(2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 2-(7-acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 20c (1.2 g, 5.15 mmol) was dissolved in 20 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (1.25 g, 10.3 mmol) and tetraethyl titanate (4.7 g, 20.6 mmol) were added in sequence, and the mixture was refluxed at 90° C. overnight. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 20d (1.3 g, yellow viscous substance) in a yield of 75%. MS m / z(ESI):337.2[M+1] (Step 5) (R)-N-((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20e (R)-N-((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20f (R)-N-((E)-1-(2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 20d (1.3 g, 3.87 mmol) was dissolved in 15 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (15 mL, 7.5 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction solution was quenched with 10 mL of methanol and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give (R)-N-((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20e (300 mg, yellow viscous substance, yield 23.1%) and (R)-N-((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20f (530 mg, yellow viscous substance, yield 40.77%). MS m / z(ESI):339.0[M+1] (Step 6) 2-((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile (R)-N-((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20e (300 mg, 0.89 mmol) was dissolved in 2 mL of dichloromethane. Then, 3 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 2-((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 20g (208 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):235.3[M+1] (Step 7) 5-(((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 2-((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 20g (208mg, 0.89mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (240mg, 1.07mmol) were dissolved in 5mL of n-butanol, N,N-diisopropylethylamine (918mg, 7.12mmol) was added, the mixture was heated to 80℃, and reacted for 5 hours. 2-((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 20g (1.3g, 5.56mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (1.26g, 5.6mmol) were dissolved in 10mL of n-butanol, N,N-diisopropylethylamine (5.74g, 44mmol) was added, and the mixture was heated to 80°C and reacted for 5 hours. After the reaction was completed, the two reaction solutions were combined and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 20h (678 mg, white solid) in a yield of 24.8%. MS m / z(ESI):424.3[M+1] (Step 8) 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((R)-1-((R)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 20h (678 mg, 1.6 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (419 mg, 1.92 mmol) and Raney nickel (1 g) were added, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was allowed to proceed at room temperature overnight. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to give ethyl 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 20i (843 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):528.4[M+1] (Step 9) 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 20i (843 mg, 1.6 mmol) was dissolved in 12 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 10:10:1), lithium hydroxide monohydrate (671 mg, 15.99 mmol) was added, and the mixture was heated to 80 ° C. and reacted for 2 hours. After the reaction was completed, the reaction solution was cooled to room temperature and concentrated under reduced pressure to remove ethanol and tetrahydrofuran, 50 mL of water was added, and the mixture was adjusted to acidity with 1.0 M diluted hydrochloric acid, and then extracted with ethyl acetate (50 mL × 3). The organic phases were then combined, washed with 100 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 20j (720 mg, yellow viscous substance) in 90% yield. MS m / z(ESI):500.2[M+1] (Step 10) 5-(((R)-1-((R)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((R)-1-((R)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 20j (720 mg, 1.44 mmol) was dissolved in 5 mL of dichloromethane. Then, 8 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 5-(((R)-1-((R)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 20k (574 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):400.1[M+1] (Step 11) (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 5-(((R)-1-((R)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 20k (574 mg, 1.44 mmol), pentafluorophenyl diphenylphosphinate (665 mg, 1.73 mmol) and N,N-diisopropylethylamine (1.49 g, 11.55 mmol) were dissolved in 11 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=10:1) and reacted at room temperature overnight. After completion of the reaction, 50 mL of dichloromethane was added to the reaction solution, washed with water (50 mL x 3), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11R)-6-fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 20 (100 mg) was obtained in a yield of 18.22%. MS m / z(ESI):382.0[M+1] 1 H NMR (400 MHz, DMSO-d6) δ 8.77-8.68 (m, 1H), 8.50 (d, J = 7.6 Hz, 2H), 8.05 (d, J = 1.9 Hz, 1H), 6.96 (s, 1H), 6.83 (s, 1H), 6.30 (d, J = 7.6 Hz, 1H), 4.66-4.47 (m, 1H), 3.61 (s, 2H), 3.26 (d, J = 22.3 Hz, 2H), 2.88 (d, J = 16.2 Hz, 1H), 2.14 (s, 1H), 1.51-1.40 (m, 3H), 1.43-1.34 (m, 3H). Example 21 (3R,11S)-6-Fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one

[0203] [ka]

[0204] [ka]

[0205] (Step 1) 2-((R)-7-((S)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile (R)-N-((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 20f (530 mg, 1.57 mmol) was dissolved in 3 mL of dichloromethane. Then, 4 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 2-((R)-7-((S)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 21a (367 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):235.4[M+1] (Step 2) 5-(((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 2-((R)-7-((S)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 21a (367 g, 1.57 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (433 mg, 1.92 mmol) were dissolved in 5 mL of n-butanol, and N,N-diisopropylethylamine (1.62 g, 12.56 mmol) was added. The mixture was heated to 80° C. and reacted for 5 hours. 2-((R)-7-((S)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)acetonitrile 21a (831 mg, 3.55 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (800 mg, 3.56 mmol) were dissolved in 10 mL of n-butanol, N,N-diisopropylethylamine (3.67 g, 28.4 mmol) was added, and the mixture was heated to 80° C. and reacted for 5 hours. After the reaction was completed, the two reaction solutions were combined and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give ethyl 5-(((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 21b (1.0 g, white solid) in a yield of 46.18%. MS m / z(ESI):424.2[M+1] (Step 3) 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((R)-1-((S)-2-(cyanomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 21b (1.0 g, 2.36 mmol) was dissolved in 5 mL of methanol. Then, di-tert-butyl dicarbonate (618 mg, 2.8 mmol) and Raney nickel (1 g) were added, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was allowed to proceed at room temperature overnight. After completion of the reaction, the reaction solution was filtered, and the filtrate was concentrated under reduced pressure to give ethyl 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 21c (1.24 g, yellow viscous substance) in 100% yield. MS m / z(ESI):528.3[M+1] (Step 4) 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 21c (1.24 g, 2.36 mmol) was dissolved in 12 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 10:1:1), lithium hydroxide monohydrate (994 mg, 23.69 mmol) was added, and the mixture was heated to 80 ° C. and reacted for 2 hours. After the reaction was completed, the reaction solution was cooled to room temperature, and ethanol and tetrahydrofuran were removed under reduced pressure. Then, 50 mL of water was added, and 1.0 M diluted hydrochloric acid was added to adjust the mixture to acidity, followed by extraction with ethyl acetate (50 mL × 3). The organic phases were then combined, washed with 10 mL of saturated sodium chloride solution, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the crude product of 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 21d (910 mg, yellow viscous material) in 77.27% yield. MS m / z(ESI):500.2[M+1] (Step 5) 5-(((R)-1-((S)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((R)-1-((S)-2-(2-((tert-butoxycarbonyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 21d (910 mg, 1.8 mmol) was dissolved in 5 mL of dichloromethane. Then, 8 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 5-(((R)-1-((S)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 21e (728 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):400.1[M+1] (Step 6) (3R,11S)-6-Fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 5-(((R)-1-((S)-2-(2-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 21e (728 mg, 1.8 mmol), pentafluorophenyl diphenylphosphinate (840 mg, 2.18 mmol) and N,N-diisopropylethylamine (1.89 g, 14.65 mmol) were dissolved in 11 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=10:1) and reacted at room temperature overnight. After completion of the reaction, 50 mL of dichloromethane was added to the reaction solution, washed with water (50 mL x 3), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11S)-6-fluoro-3,11-dimethyl-10-oxa-2,14,18,19,22-pentaazapentacyclo[14.5.2.1 8,11 .0 4,9 .0 19,23 ]Tetracosa-1(22),4,6,8,16(23),17,20-heptaen-15-one 21 (230 mg) was obtained in a yield of 33.54%. MS m / z(ESI):382.3[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.63 (d, J = 6.4 Hz, 1H), 8.55 (d, J = 7.6 Hz, 1H), 8.47 (t, J = 5.8 Hz, 1H), 8.05 (s, 1H), 6.96-6.90 (m, 1H), 6.85 (dd, J = 10.0, 2.8 Hz, 1H), 6.37 (d, J = 7.6 Hz, 1H), 5.25-5.06 (m, 1H), 3.75 (dt, J = 15.2, 7. 7 Hz, 1H), 3.70-3.62 (m, 1H), 3.48 (dt, J = 13.3, 5.5 Hz, 1H), 3.22 (d, J = 16.6 Hz, 1H), 3.03 (d, J = 16.5 Hz, 1H), 2.17-2.05 (m, 1H), 1.49 (s, 3H), 1.43 (d, J = 7.1 Hz, 3H). Example 22 (3R)-6-Chloro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0206] [ka]

[0207] [ka]

[0208] (Step 1) 1-(5-chloro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 1-(5-chloro-2-hydroxyphenyl)ethan-1-one 22a (5.1 g, 30 mmol) was dissolved in 50 ml of N,N-dimethylformamide. Then, 3-bromo-2-methylpropene (4.86 g, 36 mmol) and potassium carbonate (8.29 g, 60 mmol) were added in sequence and reacted at room temperature overnight. After the reaction was completed, 100 mL of water was added to the reaction solution and extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (50 mL x 2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to obtain 1-(5-chloro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 22b (6.72 g, yellow oil). The product was used directly in the next reaction without further purification. MS m / z(ESI):225.0[M+1] (Step 2) 1-(5-chloro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 1-(5-chloro-2-((2-methylallyl)oxy)phenyl)ethan-1-one 22b (6.72 g, 30 mmol) was heated to 220° C., stirred and reacted for 5.5 hours. After completion of the reaction, the reaction solution was cooled to room temperature, dissolved in 100 mL of dichloromethane, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain 1-(5-chloro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 22c (6.72 g, red oily substance) in 100% yield. MS m / z(ESI):224.9[M+1] (Step 3) 1-(5-chloro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-chloro-2-hydroxy-3-(2-methylallyl)phenyl)ethan-1-one 22c (1 g, 4.46 mmol) was dissolved in 30 mL of tetrahydrofuran, and iodosuccinimide (2 g, 8.93 mmol) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The residue was dissolved by adding 30 mL of dichloromethane. It was then washed with 25% sodium bisulfite solution (20 mL x 2) and saturated sodium chloride solution (20 mL x 2) successively, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (elution system: A) to obtain 1-(5-chloro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 22d (1.34 g, white solid) in 85.9% yield. MS m / z(ESI):351.0[M+1] (Step 4) 1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 1-(5-chloro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 22d (1.34 g, 3.82 mmol) was dissolved in 20 mL of N,N-dimethylformamide, sodium azide (496 mg, 7.64 mmol) was added, and the mixture was heated to 95° C. and reacted overnight. LC-MS showed the reaction was incomplete, and the reaction was continued at 115° C. for 3 hours. After the reaction was completed, the reaction solution was cooled to room temperature, and then 50 mL of water was added and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was further separated and purified by silica gel column chromatography (eluent: A) to give 1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 22e (1 g, yellow oil) in 100% yield. MS m / z(ESI):265.9[M+1] (Step 5) (R)-N-((E)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 22e (940 mg, 3.55 mmol) was dissolved in 20 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (858 mg, 7.09 mmol) and tetraethyl titanate (3.24 g, 14.2 mmol) were added in sequence, and the mixture was reacted at 75° C. overnight. After completion of the reaction, the reaction solution was cooled to room temperature, poured into 50 mL of water, added with 50 mL of ethyl acetate, and filtered. The filtrate was concentrated under reduced pressure and extracted with ethyl acetate (50 mL×2). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 22f (360 mg, white solid) in a yield of 27.7%. MS m / z(ESI):368.9[M+1] (Step 6) (R)-N-((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 22f (360 mg, 1 mmol) was dissolved in 10 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (4 mL, 2 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain (R)-N-((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 22g (271 mg, white solid) in a yield of 73%. MS m / z(ESI):371.0[M+1] (Step 7) (1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine (R)-N-((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 22g (271 mg, 0.73 mmol) was dissolved in 3 mL of dichloromethane. Then, 4 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain (1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 22h (195 mg, white solid) in 100% yield.

[0209] MS m / z(ESI):250.0[M-16] (Step 8) 5-(((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-amine 22h (525 mg, 1.97 mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (443 mg, 1.97 mmol) were dissolved in 10 mL of n-butanol. Then, N,N-diisopropylethylamine (2.7 mL, 15.76 mmol) was added and heated to 125° C. for 5 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 22i (731 mg, yellow solid) in a yield of 81%. MS m / z(ESI):456.1[M+1] (Step 9) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 22i (731 mg, 1.6 mmol) was dissolved in 10 mL of methanol. Then, di-tert-butyl dicarbonate (420 mg, 1.93 mmol) and 10% palladium carbon (100 mg, containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was carried out at room temperature overnight. After the reaction was completed, the reaction solution was filtered through diatomaceous earth, and the filtrate was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography to obtain ethyl 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 22j (350 mg, white solid) in a yield of 41%. MS m / z(ESI):530.2[M+1] (Step 10) 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid Ethyl 5-(((1R)-1-(2-(azidomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 22j (350 mg, 0.66 mmol) was dissolved in 5 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 2:2:1), lithium hydroxide monohydrate (222 mg, 5.29 mmol) was added, and the mixture was heated to 85°C and reacted for 6 hours. After completion of the reaction, the reaction was cooled to room temperature, 15 mL of ethyl acetate was added to dilute the reaction, washed with 10% aqueous citric acid (10 mL), washed with anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 22k (330 mg, yellow oil) in 100% yield. MS m / z(ESI):502.2[M+1] (Step 11) 5-(((1R)-1-(2-(aminomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(2-(((tert-butoxycarbonyl)amino)methyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 22k (330 mg, 0.66 mmol) was dissolved in 5 mL of dichloromethane. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain 5-(((1R)-1-(2-(aminomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 22l (264 mg, white solid) in 100% yield. MS m / z(ESI):401.8[M+1] (Step 12) (3R)-6-Chloro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 5-(((1R)-1-(2-(aminomethyl)-5-chloro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 22l (264 mg, 0.66 mmol), pentafluorophenyl diphenylphosphinate (304 mg, 0.79 mmol) and N,N-diisopropylethylamine (0.5 mL, 2.44 mmol) were dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=1:1) and reacted at 35°C for 4 hours. After the reaction was completed, 10 mL of water was added to the reaction solution and extracted with ethyl acetate (10 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R)-6-chloro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 22 (50 mg) was obtained in a yield of 19.8%. MS m / z(ESI):384.0[M+1] 1H NMR (400 MHz, CDCl3) δ 9.18-9.16 (d, J = 8.0 Hz, 1H), 8.22-8.13 (m, 2H), 6.97 (s, 1H), 6.88 (s, 1H), 6.59 (s, 1H),6.25-6.23 (d, J = 8.0 Hz, 1H), 5.17-5.15 (m, 1H), 3.99-3.93 (m, 1H), 3.43-3.14 (m, 3H), 1.66-1.63 (m, 6H). [Examples 23 and 24] (2R,14R)-19-Fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7.0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 23 (2R,14S)-19-Fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 24

[0210] [ka]

[0211] [ka]

[0212] (Step 1) 1-(5-fluoro-2-hydroxy-3-((2-methylallyl)oxy)phenyl)ethan-1-one 1-(5-fluoro-2,3-dihydroxyphenyl)ethan-1-one 23a (1.7 g, 10 mmol) was dissolved in 20 mL of N,N-dimethylformamide, potassium carbonate (1.52 g, 11 mmol) was added, and the mixture was stirred at room temperature. 3-Bromo-2-methylpropene (1.35 g, 10 mmol) was dissolved in 15 mL of N,N-dimethylformamide, and then added dropwise to the above mixture, heated to 50°C, and reacted at 50°C overnight. After the reaction was completed, 100 mL of water was added to the reaction solution, and the mixture was extracted with ethyl acetate (50 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (100 mL x 2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(5-fluoro-2-hydroxy-3-((2-methylallyl)oxy)phenyl)ethan-1-one 23b (430 mg, yellow oil) in a yield of 19.1%.

[0213] (Step 2) 1-(7-Fluoro-3-(iodomethyl)-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 1-(5-Fluoro-2-hydroxy-3-((2-methylallyl)oxy)phenyl)ethan-1-one 23b (130 mg, 0.58 mmol) and sodium bicarbonate (97 mg, 1.16 mmol) were dissolved in 5 mL of acetonitrile, iodine (176 mg, 0.7 mmol) was added, and the mixture was reacted at 60°C for 4 hours, then heated to 85°C and reacted continuously for 3 hours. After the reaction was completed, the reaction solution was cooled to room temperature, 50 mL of water was added, and the solid content of sodium thiosulfate was gradually added and dissolved by stirring until the color of the solution was no longer bright, and extracted with ethyl acetate (20 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (50 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 1-(7-fluoro-3-(iodomethyl)-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 23c (190 mg, pale yellow solid) in a yield of 93.6%. MS m / z(ESI):351.0[M+1] (Step 3) 1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 1-(7-Fluoro-3-(iodomethyl)-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 23c (190 mg, 0.54 mmol) was dissolved in 3 mL of N,N-dimethylformamide. Sodium azide (71 mg, 1.09 mmol) was then added and heated to 75° C. and allowed to react overnight. After completion of the reaction, the reaction solution was cooled to room temperature, 50 mL of water was added, and extracted with ethyl acetate (30 mL×3). The organic phases were then combined, washed with saturated sodium chloride solution (30 mL), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give 1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 23d (143 mg) in 98% yield. MS m / z(ESI):266.2[M+1] (Step 4) (R)-N-((E)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylene)-2-methylpropane-2-sulfinamide 1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-one 23d (143 mg, 0.54 mmol) was dissolved in 5 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (131 mg, 1.08 mmol) and tetraethyl titanate (492 mg, 2.16 mmol) were added in sequence, and the mixture was allowed to react overnight at 95° C. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give (R)-N-((E)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylene)-2-methylpropane-2-sulfinamide 23e (90 mg, yellow liquid) in a yield of 45.5%. MS m / z(ESI):369.0[M+1] (Step 5) (R)-N-((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxo-5-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethylidene)-2-methylpropane-2-sulfinamide 23e (90 mg, 0.24 mmol) was dissolved in 5 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (1 mL, 0.48 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction was quenched with 10 mL of methanol and concentrated under reduced pressure to give (R)-N-((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxo-5-yl)ethyl)-2-methylpropane-2-sulfinamide 23f (90 mg, yellow gum) in 100% yield. MS m / z(ESI):371.4[M+1] (Step 6) (1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine (R)-N-((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxo-5-yl)ethyl)-2-methylpropane-2-sulfinamide 23f (90 mg, 0.24 mmol) was dissolved in 2 mL of dichloromethane. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to obtain (1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 23g (65 mg, white solid) in 100% yield. MS m / z(ESI):267.0[M+1] (Step 7) 5-(((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl (1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethan-1-amine 23g (65mg, 0.24mmol) and ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate 1j (55mg, 0.24mmol) were dissolved in 5mL of n-butanol, N,N-diisopropylethylamine (248mg, 1.92mmol) was added, and the mixture was heated to 130°C and reacted for 6 hours. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The obtained residue was purified by silica gel column chromatography (eluent: A) to obtain ethyl 5-(((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 23h (50 mg, yellow viscous substance) in a yield of 45.87%. MS m / z(ESI):456.3[M+1] (Step 8) 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo)[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 5-(((1R)-1-(3-(azidomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 23h (50 mg, 0.1098 mmol) was dissolved in 3 mL of methanol. Then, di-tert-butyl dicarbonate (29 mg, 0.13 mmol) and 10% palladium carbon (10 mg, containing 50% water) were added in sequence, hydrogen gas was replaced three times, a hydrogen balloon was inserted, and the reaction was allowed to proceed at room temperature overnight. After completion of the reaction, the reaction solution was filtered through diatomaceous earth, and the filtrate was concentrated under reduced pressure to give ethyl 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo)[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 23i (58 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):530.1[M+1] (Step 9) 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo)[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate ethyl 23i (58 mg, 0.11 mmol) was dissolved in 4 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 2:1:1), lithium hydroxide monohydrate (41 mg, 1.1 mmol) was added, and the mixture was heated to 85 °C and reacted for 6 hours. After completion of the reaction, the reaction was cooled to room temperature and 5 mL of ethyl acetate was added to dilute the reaction, washed with 10% aqueous citric acid solution (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 23j (55 mg, yellow gum) in 100% yield. MS m / z(ESI):502.1[M+1] (Step 10) 5-(((1R)-1-(3-(aminomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 5-(((1R)-1-(3-(((tert-butoxycarbonyl)amino)methyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 23j (55 mg, 0.11 mmol) was dissolved in 2 mL of dichloromethane. Then, 2 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to give 5-(((1R)-1-(3-(aminomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 23k (44 mg, yellow viscous substance) in 100% yield. MS m / z(ESI):402.2[M+1] (Step 11) (2R,14R)-19-Fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 23 (2R,14S)-19-Fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 24 5-(((1R)-1-(3-(aminomethyl)-7-fluoro-3-methyl-2,3-dihydrobenzo[b][1,4]dioxin-5-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 23k (44 mg, 0.11 mmol), pentafluorophenyl diphenylphosphinate (51 mg, 0.13 mmol) and N,N-diisopropylethylamine (114 mg, 0.88 mmol) were dissolved in 4 mL of a mixed solvent of dichloromethane and N-N-dimethylformamide (V:V=3:1) and reacted at room temperature overnight. After completion of the reaction, 10 mL of water was added to the reaction solution and extracted with dichloromethane (10 mL x 3). The organic phases were then combined, washed with saturated sodium chloride solution (10 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (2R,14R)-19-fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21]tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 23 (10 mg, 23.8% yield), (2R,14S)-19-fluoro-2,14-dimethyl-16,22-dioxa-3,5,7,8,12-pentaazapentacyclo[12.6.2.2 4,7 .0 6,10 .0 17,21 ]Tetracosa-1(20),4,6(10),8,17(21),18,23-heptaen-11-one 24 (4.1 mg) was obtained in 9.3% yield. twenty three MS m / z(ESI):384.1[M+1] twenty four MS m / z(ESI):384.1[M+1] Example 25 (3R,11R)-16-amino-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one

[0214] [ka]

[0215] [ka]

[0216] (Step 1) 2-((7-acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)isoindoline-1,3-dione 1-(5-fluoro-2-(iodomethyl)-2-methyl-2,3-dihydrobenzofuran-7-yl)ethan-1-one 5b (33.4 g, 100 mmol) and phthalimide potassium salt 25a (22 g, 120 mmol) were dissolved in 200 mL of N,N-dimethylformamide and reacted at 145° C. for 6 hours. After completion of the reaction, 1 L of water was added to the reaction solution and extracted with ethyl acetate (300 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to give 2-((7-acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)isoindoline-1,3-dione 25b (22 g, white solid) in 62.8% yield. MS m / z(ESI):354.1[M+1] (Step 2) (R)-N-((E)-1-(2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 2-((7-acetyl-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)isoindoline-1,3-dione 25b (21.3 g, 60 mmol) was dissolved in 250 mL of tetrahydrofuran, (R)-2-methylpropane-2-sulfinamide 1b (14.6 g, 120 mmol) and tetraethyl titanate (54.7 g, 240 mmol) were added in sequence, and the mixture was reacted at 75° C. overnight. After completion of the reaction, the reaction solution was cooled to room temperature and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain (R)-N-((E)-1-(2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide. 25c (21.4 g, yellow solid) was obtained in 78.2% yield. MS m / z(ESI):457.1[M+1] (Step 3) (R)-N-((R)-1-((R)-2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((E)-1-(2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethylidene)-2-methylpropane-2-sulfinamide 25c (21.4 g, 47 mmol) was dissolved in 250 mL of tetrahydrofuran. Then, 9-borabicyclo[3,3,1]-nonane (188 mL, 94 mmol, 0.5 mol / L) was added dropwise and reacted at room temperature overnight. After completion of the reaction, the reaction was quenched with 100 mL of methanol and concentrated under reduced pressure to give (R)-N-((R)-1-((R)-2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 25d (4.5 g, yellow gum) in 21% yield. MS m / z(ESI):459.2[M+1] (Step 4) (R)-N-((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide (R)-N-((R)-1-((R)-2-((1,3-dioxoisoindolin-2-yl)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 25d (4.5 g, 10 mmol) and hydrazine hydrate (2.5 g, 50 mmol) were dissolved in 100 mL of ethanol, refluxed, and reacted for 2 hours. After completion of the reaction, the reaction solution was filtered, the filter cake was washed with ethanol (50 mL x 3), the filtrate was concentrated under reduced pressure, 500 mL of water was added, and the mixture was extracted with ethyl acetate (200 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give (R)-N-((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 25e (2.9 g, yellow oil) in 88% yield.

[0217] MS m / z(ESI):329.1[M+1] (Step 5) tert-Butyl (((R)-7-((R)-1-(((R)-tert-butylsulfinyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)carbamate (R)-N-((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)-2-methylpropane-2-sulfinamide 25e (2.9 g, 8.84 mmol) was dissolved in 20 mL of dichloromethane, di-tert-butyl dicarbonate (2.1 g, 9.7 mmol) and triethylamine (2.4 mL, 17.68 mmol) were added, and the mixture was allowed to react at room temperature overnight. After completion of the reaction, 300 mL of water was added to the reaction solution, which was then extracted with dichloromethane (100 mL x 3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (eluent: A) to obtain tert-butyl (((R)-7-((R)-1-(((R)-tert-butylsulfinyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)carbamate 25f (1.4 g, yellow oily substance) in a yield of 33.6%.

[0218] MS m / z(ESI):329.0[M-100] (Step 6) (((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)carbamic acid tert-butyl ester (((R)-7-((R)-1-(((R)-tert-butylsulfinyl)amino)ethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl) tert-butyl carbamate 25f (1.4 g, 3 mmol) and iodine (152 mg, 0.6 mmol) were dissolved in 15 mL of a mixed solvent of tetrahydrofuran and water (V:V=4:1) and reacted at 50° C. for 3 hours. After completion of the reaction, 100 mL of water was added and extracted with ethyl acetate (15 mL×3). The organic phases were then combined, dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure to give tert-butyl (((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl)carbamate 25g (0.9 g, yellow oil) in 92% yield. MS m / z(ESI):325.1[M+1] (Step 7) 2-Amino-5-(((R)-1-((R)-2-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate tert-butyl ester (((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl) tert-butyl carbamate 25g (3.7g, 11.41mmol), tert-butyl 2-amino-5-(p-tosyloxy)pyrazolo[1,5-a]pyrimidine-3-carboxylate 25h (4.61g, 11.41mmol, prepared according to published patent WO2019023417A1) and N,N-diisopropylethylamine (11.79g, 91.25mmol) were dissolved in 35mL of tertiary butyl alcohol, heated to 100°C, and reacted overnight. After completion of the reaction, the reaction solution was concentrated under reduced pressure. The resulting residue was purified by thin layer chromatography (development system: A) to obtain tert-butyl 2-amino-5-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate 25i (2.4 g, yellow foamy solid) in a yield of 37.8%. MS m / z(ESI):557.1[M+1] (Step 8) 2-Amino-5-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 2-Amino-5-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylate tert-butyl 25i (2.4 g, 4.31 mmol) was dissolved in 25 mL of dichloromethane. Then, 10 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and the mixture was reacted at room temperature for 1 hour. After completion of the reaction, the reaction solution was concentrated under reduced pressure to give 2-amino-5-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 25j (1.73 g, yellow viscous substance) in 100% yield. MS m / z(ESI):401.1[M+1] (Step 9) (3R,11R)-16-amino-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15(22),16,19-heptaen-14-one 2-Amino-5-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid 25j (1.73 g, 4.32 mmol) was dissolved in 30 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=5:1), and pentafluorophenyl diphenylphosphinate (1.99 g, 5.18 mmol) and N,N-diisopropylethylamine (4.47 g, 34.56 mmol) were added and reacted at room temperature for 2 hours. After the reaction was completed, 100 mL of dichloromethane was added to the reaction solution. The system was washed with saturated sodium chloride solution (100 mL x 2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11R)-16-amino-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,18,21-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1 (21),4,6,8,15 (22),16,19-heptaen-14-one 25 (700 mg) was obtained in 42.37% yield. MS m / z(ESI):383.0[M+1] 1H NMR (400 MHz, DMSO-d6) δ 8.68 (d, J = 9.7 Hz, 1H), 8.28 (d, J = 5.1 Hz, 1H), 8.16 (d, J = 7.5 Hz, 1H), 6.79 (dd, J = 8.1, 2.7 Hz, 1H), 6.71 (dd, J = 10.0, 2.8 Hz, 1H), 6.09 (d, J = 7.4 Hz, 1H), 4.90 (ddd, J = 7.0, 5.0, 2.0 Hz, 1H), 3.75 (dd, J = 13.1, 9.8 Hz, 1H), 3.23 (d, J = 16.8 Hz, 1H), 3.18 (d, J = 13.1 Hz, 1H), 3.00 (d, J = 16.8 Hz, 1H), 1.58 (s, 3H), 1.51 (d, J = 7.0 Hz, 3H). Example 26 (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,21,22-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15,17,19-heptaen-14-one

[0219] [ka]

[0220] [ka]

[0221] (Step 1) 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylate ethyl (((R)-7-((R)-1-aminoethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-2-yl)methyl) tert-butyl carbamate 25g (500 mg, 1.54 mmol), ethyl 6-chloroimidazo[1,2-b]pyridazine-3-carboxylate 26a (417.33 mg, 1.85 mmol) and potassium fluoride (447.76 mg, 7.71 mmol) were dissolved in 10 mL of dimethyl sulfoxide, heated to 120 ° C, and reacted overnight. After completion of the reaction, 100 mL of water was added to the reaction solution and extracted with ethyl acetate (100 mL). The organic phase was then washed with saturated sodium chloride solution (100 mL × 2), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. The resulting residue was purified by thin layer chromatography (development system: A) to obtain ethyl 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylate 26b (230 mg, yellow foamy solid) in a yield of 29.1%. MS m / z(ESI):514.3[M+1] (Step 2) 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylic acid 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl)-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylate ethyl 26b (230 mg, 0.447 mmol) was dissolved in 7 mL of a mixed solvent of ethanol, tetrahydrofuran and water (V:V:V = 5:1:1), lithium hydroxide monohydrate (187.92 mg, 4.48 mmol) was added, and the mixture was heated to 85°C and reacted overnight. After completion of the reaction, the reaction solution was cooled to room temperature, concentrated under reduced pressure, added with 100 mL of water, acidified with 1 M dilute hydrochloric acid, extracted with ethyl acetate (100 mL), dried over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylic acid 26c (217 mg, yellow viscous substance) in 99.8% yield. MS m / z(ESI):486.2[M+1] (Step 3) 6-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazole[1,2-b]pyridazine-3-carboxylic acid 6-(((R)-1-((R)-2-(((tert-butoxycarbonyl)amino)methyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazo[1,2-b]pyridazine-3-carboxylic acid 26c (217 mg, 0.447 mmol) was dissolved in 5 mL of dichloromethane. Then, 0.11 mL of hydrogen chloride·1,4-dioxane solution (4 mol / L) was added and reacted at room temperature for 1 hour. After the reaction was completed, the reaction solution was concentrated under reduced pressure to obtain the crude product of 6-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazole[1,2-b]pyridazine-3-carboxylic acid 26d (172 mg, yellow solid). The product was used directly in the next reaction. MS m / z(ESI):386.2[M+1] (Step 4) (3R,11R)-6-Fluoro-3,11-dimethyl-10-oxa-2,13,17,21,22-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ]Tricosa-1(21),4,6,8,15,17,19-heptaen-14-one 6-(((R)-1-((R)-2-(aminomethyl)-5-fluoro-2-methyl-2,3-dihydrobenzofuran-7-yl)ethyl)amino)imidazole[1,2-b]pyridazine-3-carboxylic acid 26d (172 mg, 0.447 mmol) was dissolved in 6 mL of a mixed solvent of dichloromethane and N,N-dimethylformamide (V:V=5:1), pentafluorophenyl diphenylphosphinate (172 mg, 0.447 mmol) and N,N-diisopropylethylamine (0.461 mg, 3.57 mmol) were added, and the mixture was reacted at room temperature for 2 hours. After the reaction was completed, 50 mL of dichloromethane was added to the reaction solution, which was washed with saturated sodium chloride solution (50 mL x 3), dried over anhydrous sodium sulfate, filtered, and concentrated under reduced pressure. A liquid phase was prepared from the resulting residue (separation column: AKZONOBEL Kromasil; 250 × 21.2 mm (inner diameter); 5 μm, 20 mL / min; mobile phase A: 0.05% TFA + H2O, mobile phase B: CH3CN) and (3R,11R)-6-fluoro-3,11-dimethyl-10-oxa-2,13,17,21,22-pentaazapentacyclo[13.5.2.1 8,11 .0 4,9 .0 18,22 ] Tricosa-1(21),4,6,8,15,17,19-heptaen-14-one 26 (80 mg) was obtained in 26% yield. MS m / z(ESI):368.1[M+1] 1H NMR (400 MHz, DMSO-d6) δ 9.34 (d, J = 9.4 Hz, 1H), 8.07 (d, J = 5.3 Hz, 1H), 7.88-7.72 (m, 2H), 6.86-6.73 (m, 3H), 4.89-4.74 (m, 1H), 3.93 (dd, J = 13.5, 9.6 Hz, 1H), 3.33-3.25 (m, 2H), 3.06 (d, J = 16.9 Hz, 1H), 1.63 (s, 3H), 1.55 (d, J = 6.9 Hz, 3H). [Biological evaluation] [Test Example 1] Measurement of the compounds of the present invention in the kinase activity of TRKA, TRKB, TRKC, TRKA (G595R), TRKA (G667C) and TRKC (G623R) The following method was used to measure the inhibitory degree of the compound of the present invention against the activity of recombinant human NTRK family kinase in vitro. In this method, Cisbio's HTRF® KinEASE-TK tyrosine kinase kit (product number: 62TK0PEB) was used. The principle of this kit is based on time-resolved fluorescence resonance energy transfer (TF-FRET). The inhibition of the compound against the activity of NTRK kinase was reflected by measuring the phosphorylation degree of biotinylated polypeptide substrate mediated by NTRK kinase. For detailed experimental procedures, please refer to the kit manual. Recombinant human wild-type NTRK protein was purchased from Carna bioscience (product numbers are TRKA#08-186, TRKB#08-187 and TRKC#08-197, respectively). Recombinant human mutant NTRK proteins were purchased from SignalChem (product numbers: TRKA G595R #N16-12BG-10, TRKA G667C #N16-12CG-10, and TRKC G623R #N18-12CH-10, respectively).

[0222] The experimental method was performed according to the steps in the kit instructions. The procedure is briefly described below. The test compounds were first dissolved in DMSO to prepare a stock solution. Then, they were serially diluted with the buffer provided in the kit. The final concentration of the test compounds in the reaction system ranged from 1,000 nM to 0.004 nM. The ATP Km value concentration of each NTRK protein was measured using serially diluted ATP solutions (Sangon Biotech (Shanghai) Co., Ltd., A600311). From the obtained Km values, the ATP concentrations in the reaction system were set to 100 μM (TRKA), 10 μM (TRKB), 50 μM (TRKC), 7 μM (TRKA (G595R)), 1 μM (TRKA (G667C)), and 100 μM (TRKC (G623R)). The reaction was performed in a 384-well microplate. First, the compounds and a certain amount of the corresponding NTRK protein were added to the wells and incubated at room temperature for 5 to 10 minutes. Then, the ATP solution and the biotinylated polypeptide substrate solution were added to the reaction solution and incubated at room temperature for 60 minutes with shaking. Then, the anti-phosphorylated tyrosine antibody conjugated with europium compounds and streptavidin conjugated with modified allophycocyanin XL665 were added to the reaction solution and incubated continuously at room temperature for 1 hour with shaking. After incubation, the fluorescence intensity values ​​of each well at an excitation wavelength of 304 nm and emission wavelengths of 620 nM and 665 nM were measured in the TF-FRET mode of a microplate reader. Compared with the fluorescence intensity ratio of the control group (0.1% DMSO), the inhibition rate of the compound at each concentration was calculated, and the IC of the compound was determined by performing nonlinear regression analysis of the logarithm of the compound concentration-inhibition rate by GraphPad Prism 5 software. 50 The values ​​were obtained (see Table 1).

[0223] [Table 3]

[0224] Conclusion: The compounds of the present invention have good inhibitory effects on both wild-type and mutant TRKA, TRKB and TRKC.

[0225] [Test Example 2] BAF3 LMNA-TRKA WT and BAF3 LMNA-TRKA G667C Measurement of the cell proliferation activity of the compounds of the present invention The effect of the compound of the present invention on cell proliferation was measured using the following method. BAF3 cells were purchased from the National Infrastructure of Cell Line Resource (Beijing Headquarters). For TRKA, BAF3 cells were used to express BAF3 LMNA-TRKA. WT and BAF3 LMNA-TRKA G667C Three stable cell lines were constructed. The above three cell lines were used to measure the inhibition of compounds on cell activity. The cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, 100U penicillin and 100μg / mL streptomycin. The cells were cultured in a 5% CO2 incubator at 37℃. The activity of the cells was measured by CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega, Part No.: G7573).

[0226] The experimental method was performed according to the steps in the kit instructions. It is briefly described below. The test compounds were first dissolved in DMSO to prepare stock solutions. Then, the test samples were prepared by serial dilution with the corresponding cell culture medium. The final concentrations of the compounds ranged from 1 μM to 0.15 nM. Logarithmic growth phase cells were seeded in 96-well cell culture plates at appropriate concentrations and cultured overnight in a 37°C 5% CO2 incubator. After adding the test compound samples, the cells were continuously cultured for 72 hours. After culture, 50 μL of CellTiter-Glo detection solution was added to each well, shaken for 5 minutes, and then left to stand for 10 minutes, and the luminescence value of each well was read by a microplate reader using the luminescence mode. The inhibition rate of the compound at each concentration was calculated by comparing with the absorbance value of the control group (0.3% DMSO), and the IC of the compound in inhibiting cell proliferation was calculated by performing nonlinear regression analysis of the logarithm of the compound concentration-inhibition rate using GraphPad Prism 5 software. 50The values ​​were obtained (see Table 2).

[0227] [Table 4]

[0228] Conclusion: BAF3 LMNA-TRKA WT and BAF3 LMNA-TRKA G667C The compounds of the present invention have a significant inhibitory effect on the proliferation of the stable metastatic cell lines of .

[0229] [Test Example 3] BAF3 LMNA-TRKA G595R Measurement of the cell proliferation activity of the compounds of the present invention The effect of the compound of the present invention on cell proliferation was measured using the following method. BAF3 cells were purchased from the National Infrastructure of Cell Line Resources (Beijing Headquarters). TRKA was synthesized from BAF3 cells using the BAF3 LMNA-TRKA G595R A stable transformed cell line of was constructed. The cell line was used to measure the inhibition of the compound on cell activity. The cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum, 100U penicillin and 100μg / mL streptomycin. The cells were cultured in a 5% CO2 incubator at 37℃. The activity of the cells was measured by CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega, Part No.: G7573).

[0230] The experimental method was performed according to the steps in the kit instructions. It is briefly described below. The test compounds were first dissolved in DMSO to prepare stock solutions. Then, the test samples were prepared by serial dilution with the corresponding cell culture medium. The final concentrations of the compounds ranged from 1 μM to 0.15 nM. Logarithmic growth phase cells were seeded in 96-well cell culture plates at appropriate concentrations and cultured overnight in a 37°C 5% CO2 incubator. After adding the test compound samples, the cells were continuously cultured for 72 hours. After culture, 50 μL of CellTiter-Glo detection solution was added to each well, shaken for 5 minutes, and then left to stand for 10 minutes, and the luminescence value of each well was read by a microplate reader using the luminescence mode. The inhibition rate of the compound at each concentration was calculated by comparing with the absorbance value of the control group (0.3% DMSO), and the IC of the compound in inhibiting cell proliferation was calculated by performing nonlinear regression analysis of the logarithm of the compound concentration-inhibition rate using GraphPad Prism 5 software. 50 The values ​​were obtained (see Table 3).

[0231] [Table 5]

[0232] Conclusion: Compared with repotrectinib, BAF3 LMNA-TRKA G595 The compounds of the present invention have significant inhibitory effects on the proliferation of stable metastatic cell lines of BAF3 LMNA-TRKA. G595 It has an unexpected inhibitory effect against rectinib (2.9 to 4.1 times that of rectinib).

[0233] [Test Example 4] Measurement of the activity of the compound of the present invention in ALK kinase The following method was used to measure the inhibitory degree of the compound of the present invention on the activity of recombinant human ALK kinase in vitro. This method uses Cisbio's HTRF® KinEASE-TK tyrosine kinase kit (product number: 62TK0PEB). The principle of this kit is based on time-resolved fluorescence resonance energy transfer (TF-FRET). The inhibition of the compound on the activity of ALK kinase was reflected by measuring the phosphorylation degree of biotinylated polypeptide substrate mediated by ALK kinase. For detailed experimental procedures, please refer to the kit's instruction manual. Recombinant human ALK was purchased from SignalChem (product number was ALK#A19-11G-10).

[0234] The experimental procedure is briefly described below. The test compounds were first dissolved in DMSO to prepare a stock solution. Then, they were serially diluted with the buffer solution provided in the kit. The final concentration of the test compounds in the reaction system ranged from 1,000 nM to 0.004 nM. The ATP Km value concentration of each ALK protein was measured using serially diluted ATP solutions (Sangon Biotech (Shanghai) Co., Ltd., A600311). From the obtained Km values, the ATP concentrations in the reaction system were set to 1 μM (ALK), 10 μM (ALK (G1202R)), and 25 μM (ALK (L1196M, G1202R)). The reaction was carried out in a 384-well microplate. First, the compounds and a certain amount of the corresponding ALK proteins were added to the wells and incubated at room temperature for 5 to 10 minutes. Then, the ATP solution and the biotinylated polypeptide substrate solution were added to the reaction solution and incubated at room temperature for 60 minutes with shaking. Then, anti-phosphorylated tyrosine antibody conjugated with europium compound and streptavidin conjugated with modified allophycocyanin XL665 were added to the reaction mixture and incubated continuously with shaking for 1 h at room temperature. After incubation, the fluorescence intensity values ​​of each well at an excitation wavelength of 304 nm and emission wavelengths of 620 nM and 665 nM were measured in the TF-FRET mode of a microplate reader. The fluorescence intensity ratio 665 / 620 of each cell was also calculated. Compared with the fluorescence intensity ratio of the control group (0.1% DMSO), the inhibition rate of the compound at each concentration was calculated, and the IC of the compound was determined by per...

Claims

1. A method for producing a compound represented by general formula (I), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, comprising the steps of: The method includes: 【Chemistry 1】 subjecting the compound represented by general formula (IA) to a condensation reaction under basic conditions to obtain a compound represented by general formula (I); During the ceremony, Ring A is 【Chemistry 2】 and Ring B is 【Chemistry 3】 Selected from: L １ is -NH-CH(CH ３ )—wherein L ２ is -(CR ｄ R ｅ ) ｎ -, where R ｄ and R ｅ is a hydrogen atom; R １ and R ２ are the same or different, and each independently represents a hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -NR ５ R 6 , wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally further substituted by one or more substituents selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O; R 5 and R 6 are each independently selected from a hydrogen atom; R ３ is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; n is 1 or 2; and The method, wherein p and q are the same or different and each independently selected from 0, 1, 2, or 3.

2. R １ and R ２ are each independently selected from a hydrogen atom, a halogen, an amino, a cyano, an alkyl, an alkoxy, a haloalkyl, a haloalkoxy, a hydroxyalkyl, or an alkoxyalkyl.

3. R １ and R ２ are each independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl.

4. A method for producing a compound represented by general formula (I), or a stereoisomer or tautomer thereof, or a pharma- ceutically acceptable salt thereof, comprising the steps of: The method includes: 【Chemistry 4】 subjecting the compound represented by general formula (If) to a condensation reaction under basic conditions to obtain a compound represented by general formula (I); During the ceremony: R ｊ is selected from alkyl; and Ring A is 【Chemistry 5】 and Ring B is 【Chemistry 6】 Selected from: L １ is -NH-CH(CH ３ )—wherein L ２ is -(CR ｄ R ｅ ) ｎ -, where R ｄ and R ｅ is a hydrogen atom; R １ and R ２ are the same or different, and each independently Hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -NR ５ R 6 , wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally further substituted by one or more substituents selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O; R 5 and R 6 are each independently selected from a hydrogen atom; R ３ is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; n is 1 or 2; and The method, wherein p and q are the same or different and each independently selected from 0, 1, 2, or 3.

5. R １ and R ２ are each independently selected from a hydrogen atom, a halogen, an amino, a cyano, an alkyl, an alkoxy, a haloalkyl, a haloalkoxy, a hydroxyalkyl, or an alkoxyalkyl.

6. R １ and R ２ are each independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl.

7. A compound represented by general formula (IA) or a stereoisomer or tautomer thereof, 【Chemistry 7】 During the ceremony: Ring A is 【Chemistry 8】 and Ring B is 【Chemistry 9】 Selected from: L １ is -NH-CH(CH ３ )—wherein L ２ is -(CR ｄ R ｅ ) ｎ -, where R ｄ and R ｅ is a hydrogen atom; R １ and R ２ are the same or different, and each independently Hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -NR ５ R 6 , wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally further substituted by one or more substituents selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O; R 5 and R 6 are each independently selected from a hydrogen atom; R ３ is selected from a hydrogen atom, an alkyl or a cycloalkyl, wherein said alkyl or cycloalkyl is optionally further substituted by one or more substituents selected from halogen, hydroxy, alkoxy or cycloalkyl; n is 1 or 2; and p and q are the same or different and are each independently selected from 0, 1, 2, or 3, or a stereoisomer or tautomer thereof.

8. R １ and R ２ is independently selected from a hydrogen atom, halogen, amino, cyano, alkyl, alkoxy, haloalkyl, haloalkoxy, hydroxyalkyl, or alkoxyalkyl, or a stereoisomer or tautomer thereof.

9. R １ and R ２ is independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl, or a stereoisomer or tautomer thereof.

10. The compound is selected from the following: 【Chemistry 10】 【Chemistry 11】 8. A compound according to claim 7, or a stereoisomer or tautomer thereof.

11. A method for producing a compound represented by general formula (IA) according to claim 7, or a stereoisomer or tautomer thereof, comprising the steps of: The method includes: 【Chemistry 12】 Reacting a compound represented by general formula (Ic) in the presence of di-tert-butyl dicarbonate under hydrogen and catalytic conditions to obtain a compound represented by general formula (Id); subjecting said compound of general formula (Id) to hydrolysis under basic conditions to obtain a compound of general formula (Ie); and removing the protecting group PG from the compound represented by general formula (Ie) to obtain a compound represented by general formula (IA); During the ceremony: R ３ is selected from a hydrogen atom; R ｊ is selected from alkyl; PG is tert-butoxycarbonyl; and Ring A is 【Chemistry 13】 and Ring B is 【Chemistry 14】 Selected from: L １ is -NH-CH(CH ３ )—and L ２ is -(CR ｄ R ｅ ) ｎ -, where R ｄ and R ｅ is a hydrogen atom; R １ and R ２ are the same or different, and each independently represents a hydrogen atom, hydroxyl, halogen, nitro, cyano, alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, -NR ５ R 6 , wherein said alkyl, alkoxy, cycloalkyl, heterocyclyl, aryl or heteroaryl is optionally further substituted by one or more substituents selected from hydroxy, halogen, nitro, cyano, alkoxy, cycloalkyl, heterocyclyl, aryl, heteroaryl, ═O; R 5 and R 6 are each independently selected from a hydrogen atom; n is 1 or 2; and The method, wherein p and q are the same or different and each independently selected from 0, 1, 2, or 3.

12. R １ and R ２ are each independently selected from a hydrogen atom, a halogen, an amino, a cyano, an alkyl, an alkoxy, a haloalkyl, a haloalkoxy, a hydroxyalkyl, or an alkoxyalkyl.

13. R １ and R ２ are each independently selected from a hydrogen atom, amino, cyano, F, Cl, Br, methyl, hydroxymethyl, halomethyl, or methoxymethyl. A method for producing a compound represented by general formula (IA) according to claim 12, or a stereoisomer or tautomer thereof.

Images