Medical products and methods for making same

Abstract

To provide a nonwoven coated with nanofibrillar cellulose for use as a wound dressing.SOLUTION: A method for preparing a medical product comprises providing an aqueous dispersion of nanofibrillar cellulose, providing a nonwoven fabric, immersing the nonwoven fabric in the aqueous dispersion of nanofibrillar cellulose to form a coating on the nonwoven fabric, passing the immersed nonwoven fabric through a preset gap to define the thickness of the coating on the immersed nonwoven fabric without pressing, and dewatering the immersed nonwoven fabric, to obtain the medical product. A medical product comprises a supporting layer and an absorbent layer, where the supporting layer comprises a nonwoven fabric, and the absorbent layer comprises unpressed nanofibrillar cellulose having an average fibril diameter of 200 nm or less, where the supporting layer is coated with the absorbent layer.SELECTED DRAWING: Figure 26

Description

[Technical field]

[0001] The present application relates to a method of making a medical product and to the medical product. More particularly, the present application relates to a nonwoven fabric coated with nanofibrillar cellulose for use as a wound dressing. [Background technology]

[0002] In wound healing, it is often necessary to cover the wound with a suitable dressing to protect the wound from infection and mechanical stress and to allow the wound to heal. Many types of conventional dressings tend to stick to the wound and even damage the wound when they are moved or removed. Said conditions in the wound are not optimal for the healing process.

[0003] It is therefore desirable to have wound care products that have properties that allow better healing of wounds and do not damage the wound, and it is also desirable to be able to control the healing process. Summary of the Invention [Problem to be solved by the invention]

[0004] Wound healing is based on complex biological mechanisms in which cells produce a variety of biomolecules, such as growth factors and proteins, that drive and activate the wound healing process and its different phases.

[0005] It has been shown how a wound dressing comprising a nonwoven with a specific nanofibrillar cellulose absorbent layer can improve the wound healing process and show better handling and usability of the dressing. It has been found that said dressing can provide conditions that allow the control of the flow of active biomolecules from the wound and back, a phenomenon that is useful for activating, controlling and optimizing the wound healing process. [Means for solving the problem]

[0006] This application is Providing an aqueous dispersion of nanofibril cellulose, - providing a nonwoven fabric; - immersing the nonwoven fabric in the aqueous dispersion of nanofibril cellulose to form a coating on the nonwoven fabric; - passing the immersed nonwoven fabric through a preset gap to define the thickness of the coating on the immersed nonwoven fabric without pressing; and - dewatering the soaked nonwoven fabric; - optionally repeating said dipping and said gap passage at least once; The present invention provides a method of making a medical product, comprising obtaining the medical product by

[0007] The present application provides a medical product comprising a support layer and an absorbent layer as a coating on the support layer, wherein the support layer comprises a nonwoven fabric and the absorbent layer comprises unpressed nanofibrillar cellulose having an average fibril size (average fibril diameter) of 200 nm or less.

[0008] The present application provides a medical product for use to treat a skin wound by a method comprising applying the medical product onto a skin wound to absorb a bioactive agent from the wound, storing the bioactive agent in the medical product for a period of time, and diffusing the bioactive agent back into the wound during a later phase of the wound's healing process.

[0009] Main embodiments are characterized in the independent claims. Various embodiments are disclosed in the dependent claims. The embodiments and examples described in the claims and in the description are freely combinable with one another, unless expressly stipulated otherwise.

[0010] The impregnated medical products described herein are useful in medical applications where the material comprising nanofibril cellulose comes into contact with living tissue. Nanofibril cellulose (NFC) has been found to provide advantageous properties when applied, for example, on the skin. The nanofibril cellulose-containing products described herein are non-cytotoxic and highly biocompatible with living tissue, providing several advantageous effects. Without being bound to any particular theory, it is believed that the impregnated medical products comprising nanofibril cellulose exhibit a highly hydrophilic surface, and when the medical products are applied to skin or other tissue, for example, a skin graft wound, they absorb and retain water from the tissue, forming a water film between the medical product and the tissue to promote wound healing. The medical products may also be moistened to enhance this effect. Although the nanofibril cellulose concentration is lower in the interior of the product, the surface of the product with a higher nanofibril cellulose concentration can maintain this highly hydrophilic state. It is possible to provide the advantageous properties of the nanofibril cellulose where desired, while still obtaining a lower density and other physical properties for the product as a whole.

[0011] The higher content of nanofibrillar cellulose at and / or near the surface of the nonwoven and lower at the cross-sectional center of the nonwoven, i.e., between the surfaces, provides several functions. First, the relatively higher concentration of NFC at the surface of the nonwoven, especially when present as a coating layer with suitable thickness and / or density, creates a barrier that can block or slow the flow of substances through the barrier. For example, the barrier can restrict the flow of biomolecules so that after they enter and pass through the barrier, it takes some time for the molecules to be contained in the nonwoven with a lower NFC content. In this way, a reservoir of biomolecules with liquid retention is formed, and the substances remain active in the environment within the nonwoven. Thus, when the impregnated nonwoven is applied on a wound, the biomolecules from the wound enter the nonwoven after a certain delay and are retained in the nonwoven. Furthermore, the biomolecules may also diffuse back into the nonwoven after a period of time in the opposite direction. Without being bound to any particular theory, it is believed that this phenomenon somehow creates conditions that improve wound healing. Wound healing may be improved or accelerated by storing some of the specific biomolecules secreted into the wound when the dressing is applied and / or in the early stages of wound healing, and providing these molecules back to the wound in later phases when healing conditions change in the wound and the wound may contain a different spectrum of biomolecules. It may also be advantageous to simply remove some of the biomolecules present in the wound to the dressing to promote healing. Regardless of the exact mechanism involved, it has been found that wound healing may be promoted by using the coatings and impregnated nonwovens disclosed herein.

[0012] It has been found that the nanofibrillar cellulose layer or region acts as an absorbent layer providing the above mentioned properties. In particular, it has been found that the presence of a separate coating on top of the supporting and reinforcing nonwoven layer to provide absorbency and liquid retention is advantageous, but also that the feature of the coating layer continuing into the nonwoven while decreasing as it is impregnated improves the desired functional and mechanical properties of the medical product. On the other hand, the support properties of the nonwoven are improved, since the nonwoven is not completely impregnated with nanofibrillar cellulose.

[0013] Moreover, since the nonwoven contains more concentrated NFC regions on both sides, the medical product can be applied to a wound or other target with either side facing the target. This improves the usefulness of the product. Also, since the side not facing the wound or skin contains barrier-forming NFC, the outside of the medical product is protected from contamination, in particular from microorganisms and large molecules, proteins, fats, etc. However, gases can pass through the barrier, creating and / or maintaining less anaerobic conditions, which may improve healing conditions in the wound. Thus, there is no need to cover the medical product with a covering layer, e.g., plastic, i.e., the medical product is simple, breathable, biodegradable, and reusable, especially when used as a dressing. The medical product with two substantially identical sides can be formed by a simple process. For example, it is possible to form products in which each side is different, e.g., a product with an unpressed coating on one side and a different coating, e.g., a fully or partially pressed coating, on the other side.

[0014] The medical products disclosed herein exhibit high absorption capacity and rate, which are desired in medical applications such as wound healing. Larger sheets may be made that can be used to cover large areas.

[0015] When the impregnated product is used to cover a wound or other injury or injury, for example in a product such as a plaster, dressing, medical patch, or part of a plaster, patch, or dressing, several advantages are provided. The product shows good utility because it can be easily applied and removed without being damaged, for example without tearing. The product can also be cut to the desired size and shape without affecting its properties. The material of the impregnated product, when used to cover a wound, acts as an artificial skin, which protects the wound and loosens as the wound heals. The impregnated product does not adhere to the damaged skin in an irreversible manner like conventional materials, which are usually very difficult to remove without damaging the healed area. The condition between the impregnated product and the skin facilitates the healing of the damaged area.

[0016] The medical product may be used in the treatment of grafts, such as skin grafts. The impregnated product may be used to cover the graft area, where it acts as a protective layer. As the graft heals, the impregnated product forms a scab-like structure that promotes healing.

[0017] It has been found that wounds treated with NFC wound dressings exhibit better scar quality after healing compared to nonwoven only products or products containing other polymer supports but not NFC. [Brief description of the drawings]

[0018] [Figure 1] FIG. 1 is a schematic diagram of the experimental setup in the pre-phase. [Diagram 2] FIG. 1 is a schematic diagram of the experimental setup in the main phase. [Diagram 3] Dialysate levels of IL-1α (n=3 donors) are shown. LLOQ=lower limit of quantification. [Figure 4] Dialysate levels of IL-6 (n=3 donors) are shown. ULOQ = upper limit of quantification. [Diagram 5]Dialysate levels of MIF (n=3 donors) are shown. ULOQ = upper limit of quantification. [Figure 6] Dialysate levels of NAP-2 (n=3 donors) are shown. [Figure 7] Dialysate levels of EGF (n=3 donors) are shown. LLOQ=lower limit of quantification. [Figure 8] Dialysate levels of IFN-γ (n=3 donors) are shown. LLOQ=lower limit of quantification. [Figure 9] Dialysate levels of IL-17 (n=3 donors) are shown. LLOQ=lower limit of quantification. [Figure 10] Dialysate levels of TNF-α (n=3 donors) are shown. LLOQ=lower limit of quantification. [Figure 11] Figure 1 shows levels of IL-1α in the dialysate. A) Absolute levels of IL-1α (n=5 donors). Mean ± SD is shown (for individual donors see Figure 11). B) Relative levels of IL-1α (ΔIL-1α) in response to incubation of skin samples with and without injury with NFC dressing or Suprathel® treatment, respectively. NFC dressing and Suprathel® samples have been normalized by subtracting the no dressing control levels for each time point. Boxes represent 25%-75% percentiles and whiskers represent min-max. C) and D) show ΔIL-1α plotted comparing two time points, 6 h and 24 h, without injury (C) and with injury (D). *P<0.05, Tukey's multiple comparison test. No=no dressing, Fib=NFC dressing, Sup=Suprathel®. [Figure 12]Figure 1 shows the levels of IL-6 in the dialysate. A) Absolute levels of IL-6 (n=5 donors). Mean ± SD is shown (for individual donors see Figure 12). B) Relative levels of IL-6 (ΔIL-6) in response to incubation of skin samples with and without injury with NFC dressing or Suprathel® treatment, respectively. NFC dressing and Suprathel® samples have been normalized by subtracting the no dressing control levels for each time point. Boxes represent 25%-75% percentiles and whiskers represent min-max. C) and D) show ΔIL-6 plotted for two time points, 6 h and 24 h, without injury (C) and with injury (D). **P<0.005, Tukey's multiple comparison test. No=no dressing, Fib=NFC dressing, Sup=Suprathel®. [Figure 13] Figure 1 shows levels of MIF in the dialysate. A) Absolute levels of MIF (n=5 donors). Mean ± SD is shown (for individual donors see Figure 13). B) Relative levels of MIF (ΔMIF) in response to incubation of skin samples with and without trauma with NFC dressing or Suprathel® treatment, respectively. NFC dressing and Suprathel® samples have been normalized by subtracting the no dressing control levels for each time point. Boxes represent 25%-75% percentiles and whiskers represent min-max. C) and D) show ΔMIF plotted comparing two time points, 6 h and 24 h, with no trauma (C) and with trauma (D). *P<0.05, Tukey's multiple comparison test. No=no dressing, Fib=NFC dressing, Sup=Suprathel®. [Figure 14]Figure 1 shows levels of NAP-2 in the dialysate. A) Absolute levels of NAP-2 (n=5 donors). Mean ± SD is shown (see Figure 14 for individual donors). B) Relative levels of NAP-2 (ΔNAP-2) in response to incubation of skin samples with and without injury with NFC dressing or Suprathel® treatment, respectively. NFC dressing and Suprathel® samples have been normalized by subtracting the no dressing control levels for each time point. Boxes represent 25%-75% percentiles and whiskers represent min-max. C) and D) show ΔNAP-2 plotted comparing two time points, 6 h and 24 h, with no injury (C) and injury (D). **P<0.005, Tukey's multiple comparison test. No=no coating, Fib=NFC coating, Sup=Suprathel®. [Figure 15] IL-1α levels in NFC dressings and dialysate are shown. A) Trend plots of IL-1α levels recovered from GrowDase-treated NFC dressings after 6 and 24 hours of incubation on human skin samples (n=5 donors). Data from individual donors are shown. B) Trend plots of dialysate levels of IL-1α in corresponding NFC dressing samples. C) Correlation plots comparing IL-1α levels measured in NFC dressings with corresponding dialysate levels. Lines represent linear regression at the two time points. Dotted lines represent 95% confidence intervals. Values ​​represent corresponding P-values ​​based on Pearson correlation, *P<0.05. [Figure 16]IL-6 levels in NFC dressings and dialysate. A) Trend plots of IL-6 levels recovered from GrowDase treated NFC dressings after 6 and 24 hours of incubation on human skin samples (n=5 donors). Data from individual donors are shown. B) Trend plots of dialysate levels of IL-6 in corresponding NFC dressing samples. C) Correlation plots comparing IL-6 levels measured in NFC dressings with corresponding dialysate levels. Lines represent linear regression at the two time points. Dotted lines represent 95% confidence intervals. Values ​​represent corresponding P-values ​​based on Pearson correlation. [Figure 17] Figure 1 shows levels of MIF in NFC dressings and dialysate. A) Trend plots of MIF levels recovered from GrowDase treated NFC dressings after 6 and 24 hours of incubation on human skin samples (n=5 donors). Data from individual donors are shown. B) Trend plots of dialysate levels of MIF in corresponding NFC dressing samples. C) Correlation plots comparing MIF levels measured in NFC dressings with corresponding dialysate levels. Lines represent linear regression at the two time points. Dotted lines represent 95% confidence intervals. Values ​​represent corresponding P values ​​based on Pearson correlation. [Figure 18] Figure 1 shows levels of NAP-2 in NFC dressings and dialysate. A) Trend plots of NAP-2 levels recovered from GrowDase treated NFC dressings after 6 and 24 hours of incubation on human skin samples (n=5 donors). Data from individual donors are shown. B) Trend plots of dialysate levels of NAP-2 in corresponding NFC dressing samples. C) Correlation plots comparing NAP-2 levels measured in NFC dressings with corresponding dialysate levels. Lines represent linear regression at the two time points. Dotted lines represent 95% confidence intervals. Values ​​represent corresponding P values ​​based on Pearson correlation. [Figure 19] Examples of regulating parts that form a gap g are shown in Fig. 19A, which shows two blades that form a gap g between the blades, and Fig. 19B, which shows two rollers that form a gap g between the rollers. [Figure 20] 1 shows an example where the soaked nonwoven passes A) over two blades and B) over two rollers. [Figure 21] An example of an arrangement having an immersion reservoir, a pair of nip rollers, and a cylinder dryer and / or a non-contact dryer is shown. [Figure 22] 1 shows a visual representation of the relationship between basis weight and air permeability. The line depicts the upper specification limit for air permeability, and the curve is a polynomial fit of the measured data. [Diagram 23] A bar graph for comparison of sample thickness (μm) is shown. [Figure 24] A bar graph of sample liquid retention (%) is shown. [Diagram 25] 1 shows the correlation between NFC content and water vapor transmission rate of wound dressings. [Figure 26] 1 shows a cross-sectional view of a schematic example of a medical product including a layer of nonwoven fabric coated on both sides. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0019] In this specification, percentage values ​​are by weight (w / w) unless otherwise specified. When numerical ranges are given, the ranges are inclusive of any upper and lower limits. The open term "comprise" also includes the closed term "consisting of" as an alternative.

[0020] The present application provides medical products and methods for making medical products. Terms such as "medical product", "coated product", "impregnated product", "coated and impregnated product" or "impregnated medical product", or more specifically, "medical product coated and impregnated with nanofibril cellulose" can be used interchangeably and refer to a product comprising a nonwoven or a layer of a nonwoven treated with nanofibril cellulose as described herein. The medical product may also be referred to as a medical structure. Said impregnated product may be obtained by the method of making described herein.

[0021] The medical products obtained by the immersion and / or impregnation process are different from those obtained by coating or lamination methods, such as blade coating or lamination. Such laminated products contain separate layers, which can be detected from the final product, for example, by drying and / or by using microscopic methods, and which can even be separated by peeling. In the products obtained by the processes described herein, the nanofibril cellulose is distributed on the nonwoven as a coating, and also on the fibers of the nonwoven, and also at least partially penetrates into the nonwoven. Furthermore, the products obtained by immersion and / or impregnation have a more open structure with high air and liquid permeability. A coating layer with the desired concentration, density, thickness, and surface properties can be obtained on the nonwoven. When using the immersion and / or impregnation process, a substantially small amount of nanofibril cellulose may be used. The nanofibril portion of the product is substantially inseparable from the nonwoven. Immersion makes it possible to produce a continuous coating into the nonwoven as an impregnation. On the other hand, the products obtained by the method using vacuum or pressure are also different from the present product; because the distribution of nanofibrillary cellulose in the product is different and it is not possible to maintain the same coating layer. The present method allows to maintain a separate coating layer with the desired thickness, density, concentration, and surface properties such as smoothness and permeability described herein, which is non-uniform or partially impregnated into the nonwoven fabric, and which is fixed during dehydration. The use of vacuum or pressure would destroy these structures.

[0022] The term "medical" refers to a product or use that is used or suitable for medical purposes. A medical product may be sterile or sterilizable, and sterilization can be achieved, for example, by using temperature, pressure, moisture, chemicals, radiation, or a combination thereof. The product may be, for example, autoclaved or other methods using high temperatures may be applied, in which case the product should be able to withstand high temperatures above 100°C, for example 121°C or higher or 134°C or higher. In one example, the product is autoclaved at 121°C for 15 minutes. UV or gamma sterilization may be used. A medical product may also be suitable for, for example, cosmetic purposes.

[0023] The medical products provide improved mechanical strength and other properties, such as high tear strength (tear resistance), especially in wet conditions. By combining supporting and reinforcing nonwoven structures, such as dressing fabrics, such as gauze, with nanofibrillated cellulose, impregnated products are formed. The fabrics create a continuous supporting network, the strength of which is not significantly affected by wet conditions.

[0024] Advantageous properties of the medical product include flexibility, elasticity and reshapeability. The nanofibrillar cellulose may also exhibit favorable permeability when it contains water. These properties are useful, for example, when the impregnated product is used as a dressing for healing wounds or in other medical applications, such as for delivering therapeutic or cosmetic agents.

[0025] Flexibility is a desirable feature in many applications, such as medical applications, and flexible patches and dressings comprising nanofibrillar cellulose are useful for application onto the skin, for example to cover wounds and other injuries or injuries, such as burns.

[0026] The relatively small amount and specific distribution of nanofibrillary cellulose in the product influences its flexibility, elasticity, reshapeability and stiffness. The impregnated product has a relatively low stiffness and has an open structure that exhibits favorable air and / or liquid permeability.

[0027] The flexibility or elasticity (elongation) of the product can also be influenced by the choice of nonwoven. The nanofibril cellulose itself has low flexibility and elasticity, especially when dry. For this reason, it is important to match the nonwoven with the nanofibril cellulose network to obtain a balance between the elasticity of the nonwoven and the nanofibril cellulose network. This has been achieved in the present case, where the absorbent and support layers can be provided in a functional balance.

[0028] The method includes providing an aqueous dispersion of nanofibril cellulose, which may be nanofibril cellulose as disclosed or defined in the present disclosure. Chemically unmodified and anionically chemically modified nanofibril cellulose are preferred for most applications. The nanofibril cellulose should have an appropriate degree of fibrillation to obtain the desired properties and effects.

[0029] The content of the nanofibril cellulose in the dispersion may be 2% (w / w) or less, or 1.5% (w / w) or less, or 1.2% (w / w) or less, for example in the range of 0.5-1.5% (w / w), preferably in the range of 0.7-1.2% (w / w), or most preferably in the range of 0.8-1.0% (w / w). It has been found that when using concentrations higher than 1.2% (w / w), it is difficult to obtain even a coating, but the material tends to agglomerate, which results in a poor quality coating and a poor quality coating surface. This may also depend on the desired degree of impregnation and / or coating. Within the above ranges, the nanofibril cellulose exists as a viscous hydrogel, which has certain properties characteristic of non-Newtonian fluids and also exhibits very hydrophilic properties. Thus, the properties of the hydrogel that affect the processability and the resulting structure, such as the degree of penetration or impregnation, the adhesion to the nonwoven, the viscosity, etc., are also influenced by the concentration of the dispersion. For example, using a higher NFC concentration, the hydrogel may be stronger and better able to withstand mechanical manipulation compared to a hydrogel with a lower concentration, while a hydrogel with a lower concentration may penetrate deeper and / or faster into the nonwoven and form a thinner coating during the immersion step. The dispersion may be formed in water or water with one or more additives and may contain nanofibrillar cellulose as the only or substantially only solid material, optionally also containing any suitable additives and / or auxiliaries, or the nanofibrillar cellulose may be the only fibrous or fibrillar material in the dispersion.

[0030] In one embodiment, the nanofibril cellulose has an average fibril diameter in the range of 1 to 200 nm, for example 1 to 50 nm, and / or, when dispersed in water, has a storage modulus of 350 Pa or more, for example 350 to 5000 Pa, or preferably 350 to 1000 Pa, measured using a rotational rheometer at 25°C, a frequency of 10 rad / sec, and a strain of 2% while gradually increasing the shear stress in the range of 0.001 to 100 Pa, and a yield stress of 25 Pa or more, for example 25 to 300 Pa, preferably 25 to 75 Pa.

[0031] In one embodiment, the nanofibril cellulose comprises or is a chemically anionically modified nanofibril cellulose with an average fibril diameter of 50 nm or less, for example in the range of 1-50 nm. Such highly fibrillated and chemically modified nanofibril cellulose has been found to improve the absorption and retention of bioactive agents, as well as the barrier and mechanical properties, of the medical product. In particular, such nanofibril cellulose exhibits good low swelling properties for the absorbent layer when exposed to liquids.

[0032] The method also includes providing a nonwoven. Nonwoven or nonwoven fabric may be used interchangeably and refer herein to any suitable nonwoven, such as fabric, cloth, or similar fiber-containing material, such as gauze. The nonwoven may be sterile or nonsterile, plain or impregnated, fenestrated (perforated or slitted), or a combination thereof. The nonwoven may be provided as a nonwoven sheet or web, or the like.

[0033] Nonwovens, such as nonwoven fabrics, may be provided as flat sheets. Nonwovens have two sides, a first side and a second side opposite the first side. These sides are the sides with the largest area. The operations disclosed herein may be performed on or on one or both sides of the nonwoven, and the resulting structures described herein, such as coatings, may be present on one or both sides of the nonwoven.

[0034] The nonwoven may comprise natural, semi-synthetic or synthetic fibers, such as viscose, rayon, polypropylene, polyester, etc., or combinations thereof, such as viscose-polyester blends, or blends of cellulose (pulp) with polypropylene and / or polyester. When used as a medical dressing, the nonwoven may be made of cotton. The nonwoven may also act as a pad for a patch. In one embodiment, the nonwoven is a viscose-polyester nonwoven, such as gauze. Such nonwovens are highly porous and permeable and moderately elastic to exhibit irreversible stretch in one direction.

[0035] In one embodiment, the nonwoven is a gauze. Nonwoven gauze contains fibers pressed together to resemble a weave, which provides improved wicking and greater absorbent capacity. Compared to woven gauze, this type of gauze has the advantage of producing less lint and leaving fewer fibers on the wound when removed. Examples of nonwoven gauze dressings include gauzes made of polyester, viscose, or blends of these fibers that are stronger, bulkier, and softer than woven pads.

[0036] The nonwoven may also serve to absorb substances, for example allowing the medical product to absorb exudate, absorbing blood, plasma and other liquids that exude from a wound and storing them in one place. The nonwoven may also stop bleeding and help seal the wound. The nonwoven may also contain or absorb therapeutic or other agents.

[0037] In one embodiment, the nonwoven fabric comprises natural fibers or natural fiber-based materials, such as cotton, cellulose, linen, silk, etc. Natural fibers provide free hydroxyl groups that facilitate the attachment of the nonwoven fabric to one or more layers comprising nanofibrillar cellulose via hydrogen bonding. Semi-synthetic fibers, such as viscose, may also provide free hydroxyl groups.

[0038] In one embodiment, the nonwoven fabric comprises a natural nonwoven fabric, such as a cellulose or cotton nonwoven fabric, a synthetic or semi-synthetic nonwoven fabric, or a mixture thereof. In one example, the nonwoven fabric comprises a mixture of polypropylene and cellulose. In one example, the nonwoven fabric comprises a mixture of polypropylene, polyester and cellulose. In one example, the nonwoven fabric comprises a mixture of viscose and polypropylene. In one example, the nonwoven fabric comprises a mixture of viscose and polyester. Cellulosic fibers may be mixed with these materials. These nonwoven fabrics may include or be gauzes.

[0039] The nonwoven should be highly permeable to allow the passage of liquids. The nonwoven is not a filter and does not restrict the flow through of many polymers. The nonwoven may not be used as a filter to dewater dispersions containing nanofibrillated cellulose. The nonwoven may be porous and / or fenestrated, such as with perforations or slits. Paper or cardboard is not a nonwoven. More specifically, paper is not suitable, since it does not provide a sufficiently high tear strength at the basis weight or thickness that would be suitable for the product. The same applies to cardboard or other similar cellulose products. The difference between nonwovens and paper and cardboard is that nonwovens usually contain longer fibers, for example with an average length of 4 mm or more, such as 5 mm or more. Nonwovens usually do not contain fillers used in paper and cardboard, such as inorganic fillers, sizing agents, retention agents, etc. In one example, the nonwoven is non-cellulosic.

[0040] In one example, the nonwoven fabric is elastic. Many natural, semi-synthetic, or synthetic fibers are elastic. However, in one example, the nonwoven fabric is stiff, providing inelasticity; for example, when the nonwoven fabric comprises cotton. The nonwoven fabric may also provide reinforcement, which, for example, increases the tear strength of the medical product.

[0041] Tear strength (tear resistance) is a measure of how well a material can withstand the effects of tearing. More specifically, tear strength is a measure of how well a material resists the growth of any cut under tension. Tear resistance may be measured by the method of ASTM D 412, which may be used to measure tensile strength, modulus and elongation. Tear index may also be given, where Tear Index = Tear Strength / Basis Weight, and is usually expressed in mNm 2 It is measured in g / g.

[0042] The nonwoven fabric may have a tear strength in the range of 800 to 2000 mN. The tear index may be measured according to ISO 1974. The nonwoven fabric may have a tensile strength in the range of, for example, 0.6 to 1.5 kN / m, for example, 0.7 to 1.2 kN / m. The tensile strength may be measured according to ISO 1924-3. The nonwoven fabric may have a basis weight of 20 to 60 g / m 2 Within the range of, for example, 30 to 55 g / m 2 Or 40~50g / m 2 The basis weight may be measured according to ISO 536. The nonwoven fabric may have a density of, for example, 100 to 400 g / cm. 3 may be in the range of 160 to 330 g / cm 3 Bulk is measured according to ISO 534 and is in the range of cm 3 It can be expressed in g / g.

[0043] The nonwoven, such as the dry nonwoven, may have a thickness in the range of 100 to 1000 μm, for example 100 to 200 μm, 150 to 200 μm, 150 to 300 μm, 200 to 300 μm, 300 to 400 μm, 400 to 500 μm, 500 to 600 μm, 600 to 700 μm, 700 to 800 μm, 800 to 900 μm or 900 to 1000 μm. However, thicker nonwovens may be used, for example up to 2000 μm or 3000 μm. In one embodiment, the thickness of the nonwoven may be in the range of 100 to 200 μm, for example 100 to 120 μm, 120 to 140 μm, or 140 to 160 μm, or 160 to 190 μm. These thicknesses refer to the thickness of the nonwoven fabric prior to treatment by the present method, however, the thickness after such treatment may be the same or substantially the same, or may include additional thickness due to a coating on one or both sides of the nonwoven fabric.

[0044] The gauze may comprise a natural gauze, such as cellulose or cotton gauze, a synthetic or semi-synthetic gauze, such as viscose or polyester, or a mixture thereof, In some embodiments, the gauze comprises a mixture of polypropylene and cellulose, or a mixture of polypropylene, polyester and cellulose.

[0045] The cellulose or cellulose fiber content may be 60% (w / w) or more, or 70% (w / w) or more of the nonwoven, for example about 80% (w / w) of the nonwoven, with the remainder being synthetic fibers. In one example, the nonwoven comprises about 2 / 3 cellulose fibers and about 1 / 3 synthetic fibers, such as polypropylene fibers. In one example, the nonwoven comprises about 4 / 5 cellulose fibers and about 1 / 5 synthetic fibers. Such a combination of cellulose and synthetic fibers provides good attachment of nanofibrillar cellulose to the cellulose fibers, along with the structural and mechanical properties, such as stiffness, provided by the synthetic fibers.

[0046] The method includes impregnating the nonwoven by soaking in an aqueous dispersion of nanofibril cellulose. Soaking refers to the process of soaking, dipping, or otherwise completely covering and / or exposing the nonwoven to the dispersion of nanofibril cellulose. As the entire nonwoven, present in the form of a sheet, layer, or similar flat or laminated product, can be soaked in the nanofibril cellulose dispersion, both sides of the nonwoven are in contact with the dispersion, whereby the nanofibril cellulose begins to penetrate the nonwoven from both sides substantially simultaneously. This results in at least partial impregnation of the nonwoven with the dispersion of nanofibril cellulose.

[0047] Depending on the time the nonwoven is immersed in the aqueous dispersion of nanofibril cellulose, the concentration of the dispersion, and other process conditions and treatment steps, the nonwoven may initially be coated with nanofibril cellulose primarily at the surface and subsurface regions of the nonwoven. The middle or centre regions between the surfaces of the nonwoven receive a smaller amount of nanofibril cellulose. The nonwoven may be immersed for a sufficient time to obtain a desired impregnation result, such as a desired concentration and distribution of nanofibril cellulose within the nonwoven.

[0048] During the years of developing NFC coatings, various combinations and types of NFC and nonwovens were tested, such as nonwovens treated by filtering the NFC dispersion (e.g. by using a vacuum) or nonwovens treated by impregnation and pressing, and nonwovens with uniform distribution of NFC were obtained. However, the products obtained and their properties were found to be different from each other, and this difference also affects the effectiveness and type of treatment achieved by using said products. It was difficult to obtain nonwovens with a layer or part (especially a coating layer) of NFC at or near the surface with a higher concentration and partial impregnation. Careful control of the manufacturing conditions was required. It was also found that different preparation methods resulted in different products with significantly different properties. For example, using a coating method, the nonwoven was not impregnated, and using an impregnation method including squeezing (pressing), the nanofibrillated cellulose was uniformly distributed in the nonwoven, and a functional coating layer with a suitable thickness, structure and desired properties was not obtained. According to the disclosed method, a coating layer is obtained and nanofibril cellulose is partially impregnated into the nonwoven, for example gradually decreasing, from the surface towards the center of the nonwoven. Figure 26 shows an example of a nonwoven 32 coated on both sides with nanofibril cellulose layers 30 and 31. The nonwoven is impregnated with nanofibril cellulose with a gradually decreasing concentration (indicated by the horizontal lines) towards the center of the nonwoven 32. The nanofibril cellulose is continuous from the coating layer to the impregnation, i.e. there are no breaks in the nanofibril cellulose.

[0049] In this case, it is desirable for the nonwoven to be non-uniformly impregnated such that the content of nanofibrillar cellulose near the surface of the nonwoven is higher than the content of nanofibrillar cellulose in the center between the surfaces of the nonwoven. Certain process steps may be performed to facilitate this.

[0050] The present disclosure provides a medical product comprising a support layer and an absorbent layer, the support layer being or comprising a nonwoven fabric, and the absorbent layer being or comprising nanofibril cellulose. The absorbent layer is coating the support layer, the support layer comprises an absorbent layer coating, or the absorbent layer is present as a coating on the support layer. More specifically, the present disclosure provides a medical product comprising a nonwoven fabric (the nonwoven fabric may act as a support or support layer) and a nanofibril cellulose coating on the nonwoven fabric, the nanofibril cellulose may act as an absorbent or as an absorbent / absorbent layer. The nonwoven fabric may be at least partially impregnated with nanofibril cellulose. The nanofibril cellulose in the medical product, the nonwoven fabric, and / or the absorbent layer or coating is preferably not pressed and not vacuumed, i.e. is preferably obtained without pressing and vacuum.

[0051] It was found that when a nonwoven fabric is immersed with the dispersion for a controlled time, its surface receives a higher content of NFC than the content in the area between the surfaces, i.e., inside the nonwoven fabric. "Surface" refers to the portion of the nonwoven fabric in the cross-sectional direction that is located at or near each surface of the nonwoven fabric. NFC also accumulates on the surface of the nonwoven fabric. The accumulated NFC may form a coating on the nonwoven fabric or may be present as a coating on the nonwoven fabric, such as a coating layer. The nanofibril cellulose, especially the nanofibril cellulose in the coating layer, forms an absorbent / absorbent portion or layer, which may provide the functionality disclosed herein.

[0052] A dispersion of nanofibrillar cellulose (which may be at a concentration as disclosed herein) is provided. The dispersion may be provided in or into a basin or other suitable container. The container is open to allow nonwovens to be fed into and out of the container. The container contains an amount of NFC dispersion to allow nonwovens to be immersed in the dispersion. The container may be filled with the dispersion continuously or batchwise.

[0053] The nonwoven fabric or web may be immersed in the aqueous dispersion of nanofibril cellulose for a time sufficient to obtain a desired level of basis weight and / or thickness of the coating and / or a desired level of impregnation of the nonwoven (e.g., non-uniform impregnation as described herein). The web speed may be, for example, about 0.3-1 m / min. The nonwoven fabric's dwell time in the bowl or immersion bath may be in the range of 15-90 seconds. However, the immersion time may be adjusted depending on the need and the application of the material. The method may include immersing the nonwoven fabric in the aqueous dispersion of nanofibril cellulose for, for example, 15-90 seconds. It may be preferable not to use too long an immersion time to avoid impregnating every corner of the nonwoven fabric. However, too short an immersion time may result in an incomplete or non-uniform coating and insufficient or no impregnation of the nonwoven fabric.

[0054] In the dipping step, therefore, a coating is obtained, formed or laid on the nonwoven, for example on one or both sides of the nonwoven, more particularly obtained from a wet dispersion or hydrogel. This initial coating has an initial or first thickness, which may not be the desired thickness and / or the thickness of the intermediate product obtained after dehydration and / or the thickness of the final product. The initial coating may also have a surface that is not optimal for the final product and needs to be modified. However, since it is usually desired to maintain the density and structure of the coating formed by dipping, it is preferable to avoid any method steps that include pressing, vacuum and / or other operations that would modify the density and structure of the coating. It may be desired to maintain such an unmodified coating on only one or both sides of the nonwoven.

[0055] If the soaked nonwoven is allowed to dry or stand for a while, the surface of the soaked nonwoven becomes less permeable to further impregnation, which is due to the so-called skin formation, which is characteristic of, for example, nanofibril cellulose. However, since the aqueous dispersion of nanofibril cellulose is in the form of a hydrogel and is relatively viscous and thick, the soaked nonwoven contains a large amount of gel-like dispersion, some of which may need to be removed. In order to avoid impregnating every corner of the nonwoven, it is also desirable not to press or squeeze the dispersion into the nonwoven. Such pressing or squeezing would also destroy the NFC layer, i.e. the coating layer, on the top of the nonwoven. Moreover, since the NFC is present on the surface as a viscous hydrogel, it can escape from the pressure to the sides of the press in such a pressing step, resulting in at least partial loss of the NFC.

[0056] The soaked nonwoven is passed through a predefined gap, for example between a pair of rolls, plates or blades, which does not exert substantial pressure on the nonwoven but removes excess NFC hydrogel accumulated on the nonwoven. The initial coating is substantially cut in the gap to remove excess material, i.e. the outer part of the initial coating is peeled off. The soaked nonwoven is thus passed through a predefined gap to define the thickness of the coating of the soaked nonwoven comprising nanofibrillar cellulose. The thickness of the soaked nonwoven can also be defined. The predefined gap is designed so as not to create pressure on the soaked nonwoven or on the initial coating on at least one side, such as the pressures described herein. For this reason, the passage through the gap and other method steps involving handling of the intermediate product are carried out without a press, preferably also without using a vacuum. The preset gap may have a width equal to or greater than the thickness of the nonwoven, e.g., equal to or greater than the thickness of the nonwoven in a stretched and / or immersed state, after which an intermediate coating having an intermediate or second thickness is formed.

[0057] Nanofibrillar cellulose is present as a viscous hydrogel on the surface of the nonwoven, so passing through the gap mainly removes excess hydrogel and defines the thickness of the product and / or coating. NFC hydrogel is a viscous material that is not pressed or squeezed in the manner disclosed herein. As a result, an unpressed coating layer remains on the nonwoven, which is finally dehydrated in a dehydration step, resulting in a functional coating layer with the required thickness in the final product. If the coating is pressed during processing, there is essentially no separate coating layer, especially one that may exhibit similar absorption properties as the unpressed coating layer of the present invention.

[0058] As a result, an unpressed layer of nanofibrillated cellulose is formed, defined or modified on the nonwoven, preferably with a desired thickness. The unpressed layer may be formed on one or both sides of the nonwoven. The unpressed layer may also be called a laid layer. A dipped and passed nonwoven of the desired thickness and / or density is obtained. The surface of the coating after passing through the gap is smooth and remains smooth even after subsequent dehydration. This has advantages in the use of the product and in the function of the product. A smooth surface allows it to set perfectly on the target, such as the skin or wound, and to make good contact with the target. The surface treated by passing through the gap also has the desired permeability for gases and molecules or other substances. This facilitates the formation of a condition between the tissue and the NFC coating of the medical product, and also facilitates and enhances the transfer of agents or substances between them.

[0059] Unpressed can refer to the form of nanofibrillar cellulose in the medical product and / or the form of the medical product as a whole, and also to a method of preparation that does not include a step that results in pressing the nanofibrillar cellulose dispersion and / or hydrogel and / or nonwoven to an extent that it would be compressed or pressed into the nonwoven. The term non-pressed may also be used. The above undesirable method steps also include vacuum. In said preparation, non-squeezing and non-vacuuming methods are generally used that include gap-defining the thickness of the soaked nonwoven and / or coating, but do not press the nanofibrillar cellulose hydrogel into the nonwoven and / or press the coating on the surface of the nonwoven. Thus, the coating layer remains on the surface of the fabric and is preferably not compressed. The unpressed form is not molded or obtained by pressing or pressure.

[0060] "Unpressed" may include not being pressed into the nonwoven. "Unpressed" may also refer to not being squeezed. Unpressed coating may refer to a coating obtained by dipping, which is preferably modified by a method that does not disturb the structure formed in the dipping step. Preferably, a structure is obtained that is mostly or substantially located on the top of the nonwoven. In particular, the unpressed layer of nanofibril cellulose is not forced into the nonwoven by applying a vacuum, pressure or by squeezing. However, some of the nanofibril cellulose dispersion has entered the nonwoven during dipping. The content of nanofibril cellulose preferably gradually decreases from the surfaces of the nonwoven towards the center of the nonwoven, which is located between the surfaces (Figure 26). This can be detected in the final product by using microscopic methods, which are preferably combined with dyeing of said product. Thus, the final product comprises a nanofibril cellulose coating and nanofibril cellulose impregnated within the nonwoven. However, the coated portion comprises a higher concentration of NFC compared to the inner portion of the nonwoven. The coating may comprise nanofibril cellulose as the only or substantially only solid material, optionally further comprising any suitable additives and / or auxiliaries, or nanofibril cellulose may be the only fibrous or fibrillar material in the coating (particularly when the product is obtained directly from a manufacturing process). However, the coating acting as an absorbent during and / or prior to use may also comprise other agents, such as bioactive molecules, pharmaceuticals, additives, and / or similar agents. Such agents may be added to the medical product in a further method step. Areas near the surface, such as just below the surface of the nonwoven, may also have a higher NFC concentration, for example 2-fold higher, 5-fold higher, or 10-fold higher, compared to the NFC concentration in the center or centre of the nonwoven.

[0061] The immersion may be performed for a suitable time to obtain the desired degree of impregnation, basis weight, distribution, and / or desired build-up of the NFC dispersion on the nonwoven. The method may include allowing the nonwoven to dry or rest for 0-60 seconds after immersion, which may be, for example, 1-60 seconds, 5-60 seconds, 1-30 seconds, 1-10 seconds, 1-5 seconds, or even 0-1 second if it is desired to limit the degree of impregnation. "After immersion" may refer to the situation where the nonwoven is still in a soaking, bathing, or other immersion process, or may refer to the situation where the immersed nonwoven has been removed from the immersion step, i.e., the nonwoven is no longer in contact with the immersion dispersion. After this, the soaked nonwoven may be fed to a next step, which may involve removing excess hydrogel, for example by using one or more rolls, for example a pair of rolls, which may also treat the surface of the nonwoven to fix and smooth the resulting surface, although in a continuous process such a separate step may not be present.

[0062] The gap g is formed between two parallel objects such as regulating parts, which may be, for example, rollers, plates, blades, etc., or a combination thereof. The objects usually have straight edges or surfaces that participate in the formation of the gap. In one embodiment, the method includes passing the immersed nonwoven fabric through a pre-defined gap between a pair of rollers, between a roller and a blade, between a roller and a plate, between a blade and a plate, between a pair of plates, or between a pair of blades to define the thickness of the coating on the immersed nonwoven fabric. The rollers may be movable or fixed, and the pair of rollers may include one movable roller and one fixed roller, or both rollers may be of the same type. The width of the gap refers to the shortest distance between the surfaces of the regulating parts, such as two blades 11, 13, or two rollers 12, 14, as shown in Figures 19A and B. The gap g has a width such that the immersed nonwoven fabric is not pressed in such a way that the coating and / or uneven impregnation is disturbed. The regulating parts, such as plates and / or blades, may be arranged to minimize the pressure towards the immersed nonwoven, for example they may be arranged perpendicular or at an angle to each other or to one or more other regulating parts. The two regulating parts, having a flat or blade-like structure, may be arranged at an angle in the range of 45 to 180° to each other, for example at an angle of 60 to 180°, 45 to 90° or 90 to 180°. This allows for a specific cut away or peeling of a part of the surface layer when the immersed nonwoven passes through the gap. The gap may be arranged to cut away a part of the NFC dispersion or hydrogel present on the nonwoven, in particular arranged as described above to obtain a desired thickness of the immersed nonwoven and / or coating layer.

[0063] The gap width may depend on the thickness of the nonwoven, especially if the nonwoven is stretched in the process. The gap width may be adjustable and the method may include adjusting the gap width, preferably depending on the nonwoven selected, depending on the immersion degree and / or the immersion dispersion used, and / or depending on the desired end product, for example depending on the desired thickness of the coating layer. In one example, the regulating part does not include a roller.

[0064] Specifying the thickness of the soaked nonwoven or the thickness of the coating on the nonwoven may refer to removing excess nanofibril cellulose dispersion or hydrogel, in particular removing excess nanofibril cellulose dispersion or hydrogel from the surface of the soaked nonwoven, in order to obtain a soaked nonwoven of the desired thickness. Specifying the thickness may also include specifying the thickness of the coating layer on the surface of the soaked nonwoven, preferably to obtain a coating layer of the desired thickness comprising or consisting of the nanofibril cellulose dispersion or hydrogel.

[0065] The thickness of the immersed nonwoven defined by the gap may be less than the thickness of the immersed nonwoven prior to passing through the gap, or it may be greater than or equal to the thickness of the nonwoven, e.g., greater than or equal to the thickness of the nonwoven in a stretched and / or immersed state.

[0066] The preset gap may have a width equal to or greater than the thickness of the nonwoven, for example equal to or greater than the thickness of the nonwoven in a stretched and / or immersed state. The gap may have a width of 0-0.5 mm greater than the thickness of the nonwoven, for example 0.05-0.1 mm, 0.05-0.05 mm, or 0.05-0.01 mm greater than the thickness of the nonwoven. In some examples, the gap has a width in the range of 0.01-1.0 mm, for example 0.1-0.5 mm, 0.15-0.5 mm, or 0.2-0.3 mm. However, the width depends on the thickness of the nonwoven, the properties of the NFC dispersion and / or hydrogel, and other features of the method. The thickness and basis weight and / or other properties of the remaining coating layer can be controlled by selecting an appropriate gap and / or by adjusting the width of the gap. The method may include imparting and / or adjusting the gap width, for example, prior to inserting the soaked nonwoven into the gap and / or between soak runs or passes.

[0067] The thickness of the coating layer after passing through the gap may be at least 5 μm or at least 10 μm, such as at least 20 μm, at least 30 μm, at least 40 μm, or at least 50 μm. The thickness may refer to the medical product before or after dehydration. However, the thickness of the coating may depend on the number of passes through the immersion run, and the thickness of the coating layer may therefore vary during the process.

[0068] The method may include passing the soaked nonwoven through a gap g between a pair of rollers 12, 14, sometimes also called rolls. This step may be called rolling. For example, one or both of the rollers 12, 14 may be movable relative to the other roller, so that the gap width may be adjusted such that one roller is moved to a desired distance from the other roller and fixed in that position or distance to obtain the desired distance and width of the gap. Similar gaps may be located between other structures, such as between a roll and a blade, or between two blades, two plates, etc. Similarly, blades or other regulating parts may be movable relative to other regulating parts.

[0069] The roller may include a nip roller. Nip generally refers to the contact area where two opposing rolls meet, for example in a calender. Nip rolls or pinch rolls may be powered rolls and are commonly used to press two or more sheets together to form a laminated product. In one example, one roll is powered and the other roll is freely movable. Nip rolls may be called pinch rolls or wringers. Nip rolls may be overlapping and one roll may be freely movable. Nip rolls may be, for example, steel rolls, which may have fine grooves. The use of nip rolls has been found to be very effective in removing excess dispersion from the nonwoven. Nip rolls are very useful in industrial scale processes where a long nonwoven sheet is fed from immersion immediately to the nip rolls and further to the next step, such as a dewatering step. FIG. 20A shows how the nonwoven 10 passes between two blades 11, 13, and FIG. 20B shows how it passes through two rollers 12, 14 that roll in a direction 18, 19 following the direction 16 in which the nonwoven advances. In this method, it is desirable that the rollers do not cause squeezing of the soaked fabric, i.e. they do not substantially press the soaked fabric. This is obtained by choosing a gap width that allows to define the thickness but does not compress the nanofibrillated cellulose or press or push it into the nonwoven. The same applies to any regulating parts that may be used to form the gap.

[0070] In said method steps, the nonwoven is moved through steps such as the immersion step, the gap and / or the dewatering step, preferably by using drive rolls or other means arranged to drive or move the nonwoven, which may be connected to an actuator for moving said means, the speed of which may be adjustable.

[0071] The method comprises dewatering the impregnated nonwoven, in particular the impregnated nonwoven obtained in the previous production step (or production steps), which has passed through a gap and thus has a defined thickness and / or a defined thickness of the coating and / or a defined structure. The dewatering fixes the structure obtained and preserved in the previous method steps. It has been found that dewatering by evaporation alone may be sufficient for the present product. Dewatering by evaporation, for example by using a heat source, helps to preserve and maintain the non-uniformly impregnated structure of the coating and the nonwoven (in particular on both sides of the nonwoven), resulting in efficient and proper dewatering. In contrast, dewatering methods that deform the formed structure, for example methods using a vacuum (in particular suction through the nonwoven to form a vacuum) and / or methods using high pressure, may destroy said structure. Therefore, preferably, the dewatering does not involve vacuum and / or pressing (for example pressing through a filter or pressing at very high pressure), which may deform the nonwoven or the coating. Using such a preservative dehydration method, it was possible to obtain coatings on nonwovens that comprise a dense yet porous network of nanofibrillar cellulose that provides the desired permeability to air and liquids as well as bioactive and other molecules.

[0072] In one embodiment, the dewatering is by evaporation. The dewatering may be accomplished using non-contact drying, such as infrared, floating, or impingement dryers. Air impingement drying involves blowing hot air (e.g., 300° C.) from a gas burner at high velocity against the wet sheet.

[0073] In some cases, dewatering may be performed using contact drying, for example, contact drying with a press dryer, cylinder dryer (drying cylinder) or belt dryer. This can be done after another dewatering step, such as an evaporation step, or as the only dewatering step. If a drying cylinder is used, the surface of the product will be smooth and drying is cost-effective. In belt drying, the product is dried in a drying chamber by contact with a continuous hot steel band heated by steam or hot gas. Water from the band is evaporated by the heat from the band. In such contact drying, the coating on one side can be affected, while the coating on the opposite side can remain unpressed. Hence, the contact drying method can be useful to make a product with an unpressed NFC coating only on one side of the nonwoven.

[0074] After dehydration, a final product may be obtained, which includes a coating on the nonwoven fabric, preferably with impregnation of the nonwoven fabric with NFC, with a concentration decreasing from the surface to the center of the fabric. Alternatively, an intermediate product may be obtained, which may be subjected again to the immersion treatment described herein and passed through a gap. This may be repeated until the desired product is obtained.

[0075] In the intermediate product or final product, the thickness of the coating layer provided on the nonwoven fabric, i.e., on the outside of the nonwoven fabric, may be at least 5 μm, at least 10 μm, at least 20 μm, at least 30 μm, at least 40 μm, or at least 50 μm, for example, 5-200 μm, 5-100 μm, 5-50 μm, 10-200 μm, 10-100 μm, or 10-50 μm, etc. However, the thickness may depend on the thickness of the nonwoven fabric.

[0076] Upon dewatering, the dry matter content of the nonwoven increases, for example to at least 50% (w / w), at least 70% (w / w), at least 80% (w / w), or at least 90% (w / w), such as in the range of 90-100% (w / w), for example in the range of 90-99% (w / w). The dry matter content of the coating may also increase to the above ranges.

[0077] The thickness, density and / or structure of the unpressed and unvacuumed NFC coating, even after dehydration, is maintained at a level that provides the desired properties described herein, such as absorbent capacity, liquid retention, water vapor transmission rate, permeability, and other properties and functionality. However, the thickness of the coating layer, and possibly the thickness of the entire soaked nonwoven, may decrease upon dehydration. The resulting NFC coating has also been found to provide high wound healing properties, as described herein and demonstrated in the examples. Upon dehydration, the intermediate coating becomes the final coating, which has a final or third thickness, or other final properties.

[0078] After dehydration, the product may be cut to a desired size and packaged, preferably in a sterile package. The package may be sealed, preferably by one or more seals that are gas and / or water impermeable. The product or the cut pieces may be sterilized before packaging and / or before sealing the package. The moisture content of the product may be adjusted to a desired level before packaging and / or before sealing the package. At this point, any additive (or additives) may be added.

[0079] The method may be carried out as a batch or continuous process. It has been found that the method is very suitable to be carried out as a continuous process, since the means for immersing, the means for defining the coating thickness and the means for dewatering (particularly the means for dewatering by evaporation) can be provided in sequence and the nonwoven can be run at a relatively high speed in a system set-up including these means. This can increase the productivity of the method, saving time and money. Also, a uniform product can be obtained from such a process. In one example, the immersion, the gap passing and preferably also the dewatering are carried out as a continuous process. However, the overall process may include continuous parts as batch and / or manual operations, for example when the nonwoven is to be repeatedly subjected to a certain operation, for example when the immersion and / or the gap passing are repeated. The method may include detecting and / or measuring one or more characteristics of the product, for example the thickness of the coating or nonwoven and / or any characteristic indirectly characterizing the thickness of the coating or nonwoven or other characteristics described herein. For example, visual, photometric, acoustic, mechanical and / or other means for detecting such features may be used, such as using one or more sensors, one or more probes, one or more sources of sound or light, etc.

[0080] The method may be carried out by a suitable device setup, comprising a source of nanofibril cellulose (e.g. a container, in particular an open container allowing handling of nonwoven sheets), one or more guide means (e.g. one or more rollers) that may be provided at the source of nanofibril cellulose and / or at the path of the treated nonwoven, a gap providing means (e.g. a regulating part) that may be provided together with a means for adjusting the regulating part to define the gap g, and one or more means for dewatering the treated nonwoven. The guide means may comprise one or more moving means (e.g. a means, e.g. a roller, connected to an actuator for moving the means) for moving the nonwoven in the device setup. For example, the one or more rollers may be connected to an electric motor for rotating the rollers. The device setup may be fully or partially automated.

[0081] FIG. 21 shows an example of a continuous process, where the nonwoven 10 is first immersed in a container 22 containing the NFC dispersion 20. The nonwoven 10 is moved or guided by using one or more roll treatment rolls 24, 26, and the nonwoven advances in a direction 16 that leads to a pair of rollers 12, 14 that define a gap g. One roller 14 may be static, i.e. fixed or non-moving, and the other roller may be a rotating roll. The nonwoven then moves through a guide roll 27 onto a cylinder dryer 28, which may be a turning roll, where water evaporates from the nonwoven, and / or through a non-contact dryer 29, which may be a heat source, such as an infrared source, to finally obtain a dewatered product. At least one side of the nonwoven will have an unpressed coating. It is also possible to repeat the process on the dewatered nonwoven, or on the nonwoven that has passed through the rollers but has not been dewatered. In another example, the roller 14 can be replaced by a blade 11. The setup of FIG. 21 may be modified by one or more features described herein, such as parts or devices, for example by deleting and / or substituting any of the features presented and / or by adding any other feature or features.

[0082] The immersion, gap passing and dewatering, or immersion and gap passing, may be performed once, or, if necessary, these steps may be repeated to maximize saturation and / or obtain a desired distribution of the dispersion on and in the nonwoven. It is also possible to adjust the basis weight and / or other suitable properties of the formed product, in particular the NFC coating and / or impregnation of the product. The steps of immersion, gap passing and optionally dewatering may be collectively referred to as, for example, a "pass", immersion run or coating run. Particular properties, such as the basis weight of the product or the thickness or basis weight of the coating, may be desired. In such cases, the immersion runs are repeated until the medical product reaches the desired basis weight or coating. Thus, in one example, the steps, passes or runs are repeated at least once, i.e., immersion, gap passing and optionally dewatering are performed at least twice. In one embodiment, the steps or passes are performed 1-10 times, such as 1-6 times, 2-8 times, or 2-4 times, such as 1, 2, 3, 4, 5 or 6 times, or more. 2 Basis weight within the range of, for example, 50-60 g / m 2 or until the NFC coating layer reaches 0.1-60g / m 2 Basis weight within the range of, for example, 3 to 40 g / m 2 Or 5~20g / m 2 or any other basis weight and / or other characteristic disclosed herein is obtained. The basis weight of the coating can be determined from the product when the basis weight of the nonwoven is known. When these steps are repeated, different initial and / or intermediate coatings are obtained, such as a first, a second and optionally a third initial or intermediate coating. In general, the amount of NFC, which can be characterized by basis weight, is controlled by the gap, the NFC hydrogel concentration and the number of passes. Those skilled in the art can determine these characteristics without undue experimentation.

[0083] In one embodiment, the medical product comprises a nonwoven material impregnated with nanofibrillar cellulose. Preferably, the content of nanofibrillar cellulose decreases from the surface of the nonwoven material towards the center of the nonwoven material located between the surfaces. Such a product may be obtained by the method disclosed herein.

[0084] In one example, the medical product has a basis weight of 30 to 70 g / m 2 For example, 35 to 65 g / m 2 Within the range of, for example, 45 to 65 g / m 2 In one embodiment, which has been tested and found to be particularly suitable, the medical product has a basis weight of 50 to 60 g / m 2 In one example, the medical product, especially when sterile, has a basis weight of 50 to 55 g / m 2 is within the range.

[0085] The basis weight of the nanofibrillated cellulose in the medical product, measured as the dry weight of the product, is 1 to 60 g / m 2 For example, the range may be 1 to 40 g / m 2 For example, 3 to 40 g / m 2 , 2~20g / m 2 , 2~12g / m 2 , 5~20g / m 2 Or 5 to 15 g / m 2 It is.

[0086] In one embodiment, the medical product has a thickness, e.g. bulk thickness, in the range of 200-260 μm, particularly when anionic NFC is used, e.g. bulk thickness, in the range of 200-230.

[0087] In one example, the medical product has a density of 200 to 700 g / cm 3 Within the range of, for example, 200 to 530 kg / m 3The density may be measured as apparent bulk density according to ISO 534. In one embodiment, the medical product has an apparent bulk density of 200 to 260 kg / m 3 In one embodiment, the medical product has an apparent bulk density of 220 to 260 kg / m 3 is within the range.

[0088] In one example, the medical product has a bulk of 3.9 to 4.6 cm. 3 / g, for example, 3.9 to 4.3 cm 3 / g.

[0089] In one embodiment, the medical product has an absorption capacity of 1.5 to 2.1 g / 100 cm 2 For example, the range is 1.8 to 2.1 g / 100 cm 2 Within the range of 1.5 to 1.8 g / 100 cm 2 is within the range.

[0090] In one example, the medical product has an area change when wetted in the range of 1.9-2.5%, such as in the range of 2-2.4% or 2.0-2.2%.

[0091] In one example, the medical product has an area change when dried in the range of -3 to 0%, such as in the range of -2.8 to 0%, for example in the range of -2.8 to -0.4%. If an anionic NFC is used, the area change when dried is very low or zero, for example in the range of -0.2 to 0 or about 0.

[0092] In one embodiment, the medical product has a liquid retention in the range of 14.5-40%, for example 14.5-30%, 20-30%, or 22-30%. Medical products containing anionic NFC have higher levels of liquid retention, for example in the range of 22-30% or 25-30%. High liquid retention keeps the dressing wet for a long enough time to allow biomolecules to move on and within the dressing.

[0093] In one embodiment, the medical product has a moisture vapor transmission rate (MVTR) of 4000 to 5500 g / m as measured by SFS-EN-13726-2. 2* Within 24 hours, for example 4000-5000g / m 2* 24 hours or 4400~5000g / m 2* Within a 24 hour period.

[0094] The air permeability of the medical product, preferably in an autoclaved state, may be less than 120 ml / min, or less than 650 ml / min, such as less than 1000 ml / min or less than 2100 ml / min. However, in some instances, the air permeability may be higher, such as less than 5100 ml / min. The air permeability generally correlates with the amount of nanofibril cellulose. The higher the amount of nanofibril cellulose, the lower the air permeability. At exemplary air permeabilities of less than 600 ml / min, or less than 500 ml / min, the amount of nanocellulose is at a suitable level for many applications.

[0095] In one embodiment, the medical product includes one or more bioactive agents and / or one or more therapeutic agents. One or more bioactive agents and / or one or more therapeutic agents, such as those disclosed herein or other active agents, may be added to the medical product or may diffuse into the medical product during use, i.e., when the medical product is applied onto the skin, wound, or other target.

[0096] The term "bioactive agent" as used herein, which may also be referred to as bioactive molecule and bioactive compound, refers to a molecule or other substance that may participate in or affect a biological reaction or process, or an agent that may exhibit biological activity. Such agents include biomolecules such as proteins, lipids, nucleic acids, enzymes, hormones, growth factors and other factors, signaling molecules, antibodies, activators, inhibitors, organelles, etc., which may be of biological origin, e.g., derived from or obtained from tissues, i.e., they may be natural (non-synthetic) molecules. Bioactive agents may also include synthetic molecules, such as pharmaceuticals or other molecules. Therapeutic agents may be pharmaceutical agents, i.e., drugs, but may also be bioactive agents. Therapeutic agents are intended to provide a therapeutic effect against a disease or disorder. Therapeutic agents, especially when synthetic, may be isolated and therefore provided as pure substances. The bioactive agents described herein, on the other hand, may be obtained directly from tissues, and the exact content of such bioactive agents may not always be known. It is possible, however, to provide the bioactive agent in isolated and / or purified form.

[0097] In one embodiment, the medical product has a moisture content in the range of 0-10%. However, the product may contain at least a small amount of water, probably because it is not easy or even necessary to obtain a completely dry product. The moisture content may be in the range of 1-10% (w / w), for example in the range of 5-10% (w / w), which may be suitable for using, handling and / or storing the product, for example in terms of maintaining a certain structure of the product. In many cases, the moisture content may correspond to the environmental moisture content, which may be in the range of 5-7%.

[0098] In one embodiment, the medical product is packaged in a sealed package, preferably in the form of a medical dressing or patch. The medical product may be packaged in separate packings. The separate packings may be provided as a series of packages. Typically, such packaged products are provided in a sterile state. The sealed packings may include sealed bags or similar packages, e.g. made of plastic, paper, composite materials and / or combinations thereof, and may include a tearable portion. Prior to use, the tearable portion is torn to expose the medical product, which is preferably in a sterile form and may have a desired moisture content.

[0099] One embodiment provides a kit including a medical or cosmetic product as described herein, e.g., a packaged product, where the kit may include one or more of said packaged products. The kit may also include other materials or equipment, such as a container containing saline or the like, for pre-treating one or more of said products prior to use.

[0100] Nanofibril Cellulose The starting material for making the medical product is nanofibril cellulose, also called nanocellulose, which refers to isolated cellulose fibrils or fibril bundles from cellulose raw materials. Nanofibril cellulose is based on natural polymers that are abundant in nature. Nanofibril cellulose has the ability to form viscous hydrogels in water. Nanofibril cellulose production techniques can be based on disintegrating fibrous raw materials, such as grinding an aqueous dispersion of pulp fibers to obtain nanofibrillated cellulose. After the grinding or homogenization process, the resulting nanofibril cellulose material is a thin viscoelastic hydrogel.

[0101] The resulting material is usually present homogeneously distributed in water at a relatively low concentration due to the disintegration conditions. The starting material may be an aqueous gel at a concentration of 0.2-10% (w / w), for example 0.2-5% (w / w). The nanofibril cellulose may be obtained directly from the disintegration of fibrous raw materials. An example of a commercially available nanofibril cellulose hydrogel is GrowDex® by UPM.

[0102] Nanofibril cellulose has unique properties due to its nanoscale structure that allow functionality that cannot be provided by conventional cellulose. However, nanofibril cellulose is also a challenging material due to its nanoscale structure. For example, nanofibril cellulose can be difficult to dehydrate or handle.

[0103] Nanofibril cellulose may be produced from cellulose raw materials of plant origin or may originate from certain bacterial fermentation processes. Nanofibril cellulose is preferably made from plant materials. The raw materials may be based on any plant material containing cellulose. In one example, the fibrils are obtained from non-parenchymal plant materials. In such cases, the fibrils may be obtained from secondary cell walls. One abundant source of such cellulose fibrils is wood fibers. Nanofibril cellulose may be produced by homogenizing fibrous raw materials from wood, which may be chemical pulp and / or bleached pulp. The cellulose fibers are disintegrated to produce fibrils with an average diameter of only some nanometers (which in most cases may be 200 nm or less) and to give an aqueous dispersion of fibrils. Fibrils derived from secondary cell walls are essentially crystalline with a crystallinity of at least 55%. Such fibrils may have different properties than fibrils derived from primary cell walls, for example, dehydration of fibrils derived from secondary cell walls may be more difficult. In general, in cellulose sources from primary cell walls such as sugar beet, potato tuber and banana cob, microfibrils are easier to liberate from the fiber matrix and require less energy to disintegrate than fibrils from wood. However, these materials are still somewhat heterogeneous and consist of large fibril bundles.

[0104] The non-woody material may be from agricultural residues, grasses or other plant matter, such as straw, leaves, bark, seeds, husks, flowers, vegetables or fruits from cotton, corn, wheat, oats, rye, barley, rice, flax, hemp, abaca, sisal, jute, ramie, kenaf, bagasse, bamboo or reeds. The cellulose raw material may be derived from a cellulose-producing microorganism. The microorganism may be of the genera Acetobacter, Agrobacterium, Rhizobium, Pseudomonas or Alcaligenes, preferably of the genus Acetobacter, more preferably of the species Acetobacter xylinumor or Acetobacter pasteurianus.

[0105] Nanofibrillated cellulose obtained from wood cellulose has been found to be preferred for the medical or scientific products described herein. Wood cellulose is available in large quantities and the preparation methods developed for wood cellulose allow the production of nanofibrillated materials suitable for said products. Nanofibrillated cellulose obtained by fibrillating plant fibers, especially wood fibers, is structurally different and has different properties than nanofibrillated cellulose obtained from microorganisms. For example, compared to bacterial cellulose, nanofibrillated wood cellulose is a homogeneous, more porous and loose material, which is advantageous in applications involving living tissues. Bacterial cellulose is usually used as is without fibrillation as in the case of plant cellulose, so said material is also different in this respect. Bacterial cellulose is a dense material that tends to form small spheroids, and therefore the structure of the material is discontinuous: it is undesirable to use such material in applications involving living tissues, especially when homogeneity of the material is required.

[0106] The wood may be from a coniferous tree such as spruce, pine, fir, larch, douglas-fir, or hemlock, or from a hardwood such as birch, aspen, poplar, alder, eucalyptus, oak, beech, or acacia, or from a mixture of coniferous and hardwood trees. In one example, the nanofibril cellulose is obtained from wood pulp. The wood pulp may be bleached pulp. The nanofibril cellulose may be obtained from hardwood pulp. In one example, the hardwood is birch. The nanofibril cellulose may be obtained from coniferous pulp. In one example, the wood pulp is a chemical pulp. Chemical pulp may be desirable for the products disclosed herein. Chemical pulp is a pure material and can be used in a wide range of applications. For example, chemical pulp lacks pitch and resin acids present in mechanical pulp and is more sterile or easily sterilizable. In addition, chemical pulp is more flexible, which exhibits advantageous properties, for example, in medical and scientific materials. For example, highly homogenous nanofibrillar cellulose material can be made without excessive processing or the need for specific equipment or laborious process steps. In one example, the pulp is bleached birch pulp.

[0107] Nanofibrillar cellulose, including cellulose fibrils and / or fibril bundles, is characterized by a high aspect ratio (length / diameter). The average length of nanofibril cellulose (median length of particles such as fibrils or fibril bundles) may be greater than 1 μm and in most cases is less than 50 μm. When elementary fibrils are not completely separated from each other, the entangled fibrils may have an average total length, for example, in the range of 1-100 μm, 1-50 μm, or 1-20 μm. However, when the nanofibrillar material is highly fibrillated, the elementary fibrils may be completely or almost completely separated and the average fibril length is shorter, for example, in the range of 1-10 μm or 1-5 μm. This is especially true for native grade fibrils that have not been shortened or digested, for example chemically, enzymatically or mechanically. However, heavily derivatized nanofibril cellulose may have a shorter average fibril length, for example in the range of 0.3-50 μm, such as 0.3-20 μm, for example 0.5-10 μm or 1-10 μm. Particularly shortened fibrils, such as enzymatically or chemically digested fibrils or mechanically treated material, may have an average fibril length of less than 1 μm, for example 0.1-1 μm, 0.2-0.8 μm, or 0.4-0.6 μm. The fibril length and / or diameter may be estimated microscopically, for example using CRYO-TEM, SEM or AFM images.

[0108] The average diameter (width) of nanofibril cellulose is less than 1 μm, or 500 nm or less, for example in the range of 1-500 nm, but preferably 200 nm or less, and may even be 100 nm or less or 50 nm or less, for example in the range of 1-200 nm, 2-200 nm, 2-100 nm, or 2-50 nm, and even 2-20 for highly fibrillated materials. The diameters disclosed herein may refer to fibrils and / or fibril bundles. The smallest fibrils are on the scale of elementary fibrils, and the average diameter is typically in the range of 2-12 nm. The dimensions and size distribution of the fibrils depend on the purification method and efficiency. For highly purified native nanofibril cellulose, the average fibril diameter, including fibril bundles, may be in the range of 1-200 nm, or 1-100 nm, for example in the range of 2-100 nm, 1-50 nm, or 10-50 nm. Nanofibril cellulose is characterized by a large specific surface area and strong hydrogen bond formation ability. In aqueous dispersion, nanofibril cellulose is typically a light or hazy gel-like material. Depending on the fiber source, nanofibril cellulose obtained from plants, especially wood, may also contain small amounts of other plant components, especially wood components, such as hemicellulose or lignin. The amount depends on the plant source.

[0109] In general, cellulose nanomaterials can be classified into categories according to TAPPI W13021, which provides standard terminology for cellulose nanomaterials. Not all of these materials are nanofibrillar cellulose. The two main categories are "nano-objects" and "nano-structured materials". Nano-structured materials include "cellulose microcrystals" (sometimes called CMCs), which are 10-12 μm in diameter and have a length:diameter ratio (L / D)<2, and "cellulose microfibrils", which are 10-100 nm in diameter and 0.5-50 μm in length. Nano-objects include "cellulose nanofibers", which can be classified into "cellulose nanocrystals" (CNCs), which are 3-10 nm in diameter and L / D>5, and "cellulose nanofibrils" (CNFs or NFCs), which are 5-30 nm in diameter and L / D>50.

[0110] Various grades of nanofibrillated cellulose can be classified based on three main properties: (i) size distribution, length and diameter, (ii) chemical composition, and (iii) rheological properties. These properties may not be completely dependent on each other. They may be used in parallel to fully describe the grades. Examples of various grades include native (or chemically unmodified) NFC, oxidized NFC (high viscosity), oxidized NFC (low viscosity), carboxymethylated NFC, and cationized NFC. There are also subgrades within these main grades, such as: very high fibrillation vs. moderate fibrillation, high substitution vs. low substitution, low viscosity vs. high viscosity, etc. Fibrillation techniques and chemical pre-modifications affect fibril size distribution. Typically, the non-ionic grades have a wider average fibril diameter (e.g., in the range of 10-100 nm, or 10-50 nm), while the chemically modified grades are much finer (e.g., in the range of 2-20 nm). The distribution is also narrower for the modified grades. Certain modifications, notably TEMPO oxidation, produce shorter fibrils.

[0111] Depending on the raw material source, e.g., hardwood pulp vs. softwood pulp, different polysaccharide compositions will be present in the final nanofibril cellulose product. Generally, non-ionic grades are made from bleached birch pulp, which gives a high xylene content (25% by weight). Modified grades are made from either hardwood or softwood pulp. In these modified grades, hemicelluloses are also modified along with the cellulose domains. Almost certainly the modification is not homogenous, i.e., some parts are more modified than others. Therefore, the modified products are a complex mixture of different polysaccharide structures, and detailed chemical analysis is usually not possible.

[0112] In an aqueous environment, dispersions of cellulose nanofibers form viscoelastic hydrogel networks. The gel is already formed at relatively low concentrations, e.g., 0.05-0.2% (w / w), by dispersed and hydrated entangled fibrils. The viscoelastic properties of NFC hydrogels can be characterized, for example, by dynamic oscillatory rheology measurements.

[0113] Nanofibril cellulose hydrogels exhibit distinctive rheological properties. Nanofibril cellulose dispersions are non-Newtonian fluids that do not follow Newton's law of viscosity, i.e., a law of constant viscosity independent of stress. Nanofibril cellulose dispersions exhibit shear thinning, meaning that the viscosity depends on the rate (or force) of deformation of the material. This is a special case of thixotropic behavior, which is time-dependent shear thinning, and when subjected to a sudden change in shear rate, it takes a finite time to achieve equilibrium viscosity. More specifically, nanofibril cellulose dispersions are pseudoplastic fluids that return to a gel state almost immediately when the shear stress is removed. Conventional Newtonian materials, such as conventional cellulose, do not exhibit such behavior, and therefore teachings on such Newtonian materials cannot generally be applied to nanofibril cellulose.

[0114] When measuring viscosity in a rotational rheometer, shear thinning behavior is seen as a decrease in viscosity with increasing shear rate. The hydrogels exhibit plastic behavior, meaning that a certain shear stress (force) is required before the material begins to flow easily. This critical shear stress is often called the yield stress. The yield stress can be determined from the steady-state flow curve measured by a stress-controlled rheometer. When the viscosity is plotted as a function of applied shear stress, a sharp decrease in viscosity is seen after the critical shear stress is exceeded. Zero shear viscosity and yield stress are the most important rheological parameters to describe the suspending power of the material. These two parameters very clearly separate different grades, thus allowing the classification of grades.

[0115] The size of the fibrils or fibril bundles depends, for example, on the feedstock, the disintegration method, and the number of disintegration runs. Mechanical disintegration of the cellulosic feedstock can be performed by any suitable equipment, such as refiners, grinders, dispersers, homogenizers, colloiders, attrition grinders, pin mills, rotor-rotor dispersers, ultrasonic sonicators, fluidizers such as microfluidizers, macrofluidizers, or fluidizer-type homogenizers. The disintegration process is performed in the presence of sufficient water to prevent the formation of bonds between the fibers.

[0116] In one example, the disintegration is performed by using a disperser having at least one rotor, blade or similar moving mechanical member, for example, by using a rotor-rotor disperser having at least two rotors. In the disperser, the fibrous material in the dispersion is repeatedly impacted by the blades or ribs of the rotor, which strike the fibrous material from opposite directions, as the blades rotate in opposite directions at a rotational speed and at a peripheral speed determined by the radius (distance to the axis of rotation). As the fibrous material moves radially outward, it impacts on the broad surfaces of the blades or ribs that come from opposite directions at a high peripheral speed; in other words, the fibrous material is subjected to multiple successive impacts from opposite directions. Also, shear stresses are generated at the edges of the broad surfaces of the blades or ribs that form the blade gaps with the opposing edges of the next rotor blade, which contribute to the disintegration of the fibers and the detachment of the fibrils. Collision frequency is determined by the rotational speed of the rotors, the number of rotors, the number of blades on each rotor, and the flow rate of the dispersion through the device.

[0117] In rotor-rotor dispergators, the fibrous material is introduced through counter-rotating rotors radially outward relative to the rotor axes such that the fibrous material is subjected to repeated shear and impact forces by the effect of the counter-rotating rotors and is thereby simultaneously fibrillated. One example of a rotor-rotor dispergator is the Atrex device.

[0118] Another example of a device suitable for disintegration is a pin mill, such as a multi-peripheral pin mill. One example of such a device includes a housing and a first rotor with an impact surface inside the housing; a second rotor concentric with the first rotor and with an impact surface, the second rotor being arranged to rotate in the opposite direction to the first rotor; or a stator concentric with the first rotor and with an impact surface. The device includes a feed orifice located in the housing and opening into the center of the rotors or the center of the rotor and the stator, and a discharge orifice located on the housing wall and opening into the periphery of the outermost rotor or stator.

[0119] In one example, the disintegration is carried out by using a homogenizer, in which the fiber material is subjected to homogenization under the effect of pressure. The forced flow of the fiber material dispersion homogenizes the fiber material dispersion into nanofibrillary cellulose, which disintegrates the material into fibrils. The fiber material dispersion passes at a given pressure through a narrow flow-through gap, where an increase in the linear velocity of the dispersion creates shear and collision forces on the dispersion, which results in the removal of fibrils from the fiber material. The fiber fragments are disintegrated into fibrils in a fibrillation step.

[0120] The term "fibrillation" as used herein generally refers to the mechanical disintegration of fibrous material by a treatment applied to the particles, in which the cellulose fibrils are separated from the fibers or fiber fragments. The treatment may be based on various effects such as grinding, crushing or shearing, or a combination of these, or another corresponding action that reduces the particle size. The expressions "disintegration" or "disintegration treatment" may be used interchangeably with "fibrillation".

[0121] The fibrous material dispersion subjected to fibrillation is a mixture of fibrous material and water, also referred to herein as "pulp". Although fibrous material dispersion may generally refer to whole fibers, parts (fragments) separated therefrom, fibril bundles, or fibrils mixed with water, typically aqueous fibrous material dispersions are mixtures of the above elements, the ratio of said components depending on the degree of processing or processing stages, for example the number of runs or "passes" in the overall processing of the same batch of fibrous material.

[0122] One way to characterize nanofibril cellulose is to use the viscosity of an aqueous solution containing nanofibril cellulose. The viscosity may be, for example, Brookfield viscosity or zero shear viscosity. The specific viscosity described herein distinguishes nanofibril cellulose from non-nanofibril cellulose.

[0123] In one example, the apparent viscosity of nanofibril cellulose is measured by a Brookfield Viscometer (Brookfield Viscometer) or another corresponding device. Preferably, a vane spindle (#73) is used. There are several commercially available Brookfield Viscometers available for measuring the apparent viscosity, all based on the same principle. Preferably, an RVDV spring (Brookfield RVDV-III) is used in said device. A sample of nanofibril cellulose is diluted to a concentration of 0.8% by weight in water and mixed for 10 minutes. The diluted sample mass is added to a 250 ml beaker, the temperature is adjusted to 20°C ± 1°C, heated if necessary and mixed. A low rotation speed of 10 rpm is used. Generally, the Brookfield viscosity can be measured at a concentration of 0.8% (w / w) and 10 rpm at 20°C ± 1°C.

[0124] Nanofibril cellulose, for example the nanofibril cellulose provided as starting material in the method, can be characterized by the viscosity it gives in an aqueous solution. The viscosity describes, for example, the degree of fibrillation of the nanofibril cellulose. In one example, the nanofibril cellulose, when dispersed in water and measured at a concentration of 0.8% (w / w) and at 10 rpm at 20°C ± 1°C, gives a Brookfield viscosity of at least 2000 mPa·s, for example a Brookfield viscosity of at least 3000 mPa·s. In one example, the nanofibril cellulose, when dispersed in water and measured at a concentration of 0.8% (w / w) and at 10 rpm at 20°C ± 1°C, gives a Brookfield viscosity of at least 10000 mPa·s. In one example, the nanofibril cellulose, when dispersed in water and measured at a concentration of 0.8% (w / w) and at 10 rpm at 20°C ± 1°C, gives a Brookfield viscosity of at least 15000 mPa·s. Examples of Brookfield viscosity ranges of the nanofibril cellulose when dispersed in water, measured at a concentration of 0.8% (w / w) and 10 rpm at 20°C ± 1°C, include 2000 to 20000 mPa·s, 3000 to 20000 mPa·s, 10000 to 20000 mPa·s, 15000 to 20000 mPa·s, 2000 to 25000 mPa·s, 3000 to 25000 mPa·s, 10000 to 25000 mPa·s, 15000 to 25000 mPa·s, 2000 to 30000 mPa·s, 3000 to 30000 mPa·s, 10000 to 30000 mPa·s, and 15000 to 30000 mPa·s.

[0125] Nanofibril cellulose may also be characterized by its average diameter (or width), or by its average diameter together with its viscosity, such as Brookfield viscosity or zero shear viscosity. In one example, nanofibril cellulose suitable for use in the products described herein has an average fibril diameter in the range of 1-200 nm, or 1-100 nm. In one example, the nanofibril cellulose has an average fibril diameter in the range of 1-50 nm, such as 2-20 nm or 5-30 nm. In one example, the nanofibril cellulose has an average fibril diameter in the range of 2-15 nm, such as in the case of TEMPO oxidized nanofibril cellulose.

[0126] Fibril diameter can be determined by several techniques, including by microscopy. Fibril thickness and width distribution can be measured by image analysis of images from a field emission scanning electron microscope (FE-SEM), a transmission electron microscope (TEM), such as a cryo-transmission electron microscope (cryo-TEM), or an atomic force microscope (AFM). In general, AFM and TEM are best suited for nanofibrillar cellulose grades with a narrow fibril size distribution.

[0127] The rheometric viscosity of nanofibrillar cellulose dispersions can be measured at 22° C., according to one example, by a stress-controlled rotational rheometer (AR-G2, TA Instruments, UK) with a narrow gap vane geometry (diameter 28 mm, length 42 mm) in a cylindrical sample cup with a diameter of 30 mm. After the sample is introduced into the rheometer, it is left to stand for 5 minutes before the measurement is started. The steady-state viscosity is measured at gradually increasing shear stress (proportional to the applied torque) and the shear rate (proportional to the angular velocity). The viscosity indicated at a certain shear stress (=shear stress / shear rate) is recorded after a constant shear rate is reached or after a maximum time of 2 minutes. The measurement is performed at a shear rate of 1000 s -1 This method can be used to determine the zero shear viscosity.

[0128] In another example, rheological measurements of hydrogel samples were performed using a stress-controlled rotational rheometer (AR-G2, TA instruments, UK) with a 20 mm plate geometry. The samples, without dilution, were loaded into the rheometer with a 1 mm gap and allowed to stand for 5 min before measurements were started. Stress sweep viscosity was measured at 25°C, at a frequency of 10 rad / s, strain of 2%, and gradually increasing shear stress in the range of 0.001 to 100 Pa. Storage modulus, loss modulus, and yield stress / break strength can be determined.

[0129] In one example, the nanofibril cellulose, for example the nanofibril cellulose provided as a starting material in the method, when dispersed in water and measured by rotational rheometer at a concentration of 0.5% by weight (w / w) in aqueous medium at 22° C.±1° C., provides a zero shear viscosity (a "plateau" of constant viscosity at low shear stress) in the range of 1000 to 100000 Pa·s, for example in the range of 5000 to 50000 Pa·s, and a yield stress (shear stress at which shear thinning begins) in the range of 1 to 50 Pa, for example in the range of 3 to 15 Pa. Such nanofibril cellulose may also have an average fibril diameter of 200 nm or less, for example in the range of 1 to 200 nm.

[0130] Turbidity is the opacity or cloudiness of a fluid caused by individual particles (a totality of suspended or dissolved solids) that are generally not visible to the naked eye. There are several practical ways to measure turbidity, the most direct being some measure of the attenuation (i.e., decrease in intensity) of light as it passes through a sample column of water. An alternative method used, the Jackson Candle method (units: Jackson Turbidity Units or JTU), is essentially an inverse measurement of the length of a column of water required to completely obscure a candle flame seen through the column of water.

[0131] Turbidity can be quantitatively measured using optical turbidity measuring instruments. There are several commercially available turbidimeters for quantitatively measuring turbidity. In the present case, a nephelometric method is used. The unit of turbidity derived from a calibrated nephelometer is called Nephelometric Turbidity Units (NTU). The measuring device (turbidimeter) is calibrated and controlled by a standard calibration sample, and then the turbidity of a diluted NFC sample is measured.

[0132] In one turbidity measurement method, a nanofibril cellulose sample is diluted in water to a concentration below the gel point of the nanofibril cellulose, and the turbidity of the diluted sample is measured. The concentration at which the turbidity of the nanofibril cellulose sample is measured is 0.1%. A HACH P2100 turbidimeter with a 50 ml measuring vessel is used for the turbidity measurement. The dry content of the nanofibril cellulose sample is determined, and 0.5 g of the sample calculated as dry content is placed in the measuring vessel, which is filled with tap water to 500 g and mixed vigorously by shaking for about 30 seconds. This aqueous mixture is immediately divided into five measuring vessels inserted in the turbidimeter. Three measurements are made for each vessel. The mean value and standard deviation are calculated from the obtained results, and the final result is given in NTU.

[0133] One way to characterize nanofibrillated cellulose is to define both viscosity and turbidity. Small fibrils scatter less light, so low turbidity refers to small fibril size, such as small diameter. In general, as the degree of fibrillation increases, the viscosity increases and the turbidity decreases at the same time. This occurs, however, up to a certain point. If fibrillation continues further, the fibrils eventually begin to break and can no longer form a strong network structure. Thus, after this point, both turbidity and viscosity begin to decrease.

[0134] In one example, the turbidity of anionic nanofibril cellulose, measured at a concentration of 0.1% (w / w) in an aqueous medium and measured by nephelometry, is less than 90 NTU, such as 3-90 NTU, such as 5-60, such as 8-40. In one example, the turbidity of native nanofibril cellulose, measured at a concentration of 0.1% (w / w) in an aqueous medium and measured by nephelometry, can even be more than 200 NTU, such as 10-220 NTU, such as 20-200, such as 50-200. These ranges may be combined with the viscosity ranges of nanofibril cellulose, such as zero shear viscosity, storage modulus, and / or yield stress, to characterize the nanofibril cellulose.

[0135] The nanofibril cellulose may be or may include unmodified nanofibril cellulose. Modification may refer to chemical, enzymatic and / or physical modification. The discharge of unmodified nanofibril cellulose is significantly faster than, for example, anionic grades. Unmodified nanofibril cellulose generally has a Brookfield viscosity measured at 20°C ± 1°C at a concentration of 0.8% (w / w) and 10 rpm in the range of 2000 to 10000 mPa·s. The nanofibril cellulose preferably has a suitable carboxylic acid content, for example, a carboxylic acid content determined by conductometric titration in the range of 0.6 to 1.4 mmol COOH / g, for example, in the range of 0.7 to 1.2 mmol COOH / g, or in the range of 0.7 to 1.0 mmol COOH / g or 0.8 to 1.2 mmol COOH / g.

[0136] The disintegrated fibrous cellulose raw material may be a modified fibrous raw material, which is a raw material whose fibers have been affected by a treatment such that the cellulose nanofibrils are more easily separable from the fibers. The modification is usually carried out on a fibrous cellulose raw material that is present as a suspension in a liquid, i.e. a pulp.

[0137] The modification treatment of the fibers may be chemical, enzymatic or physical. In chemical modification, the chemical structure of the cellulose molecule is changed by chemical reactions ("derivatization" of cellulose), preferably resulting in the addition of functional groups to the β-D-glucopyranose units of the polymer, while the length of the cellulose molecule is not affected. The chemical modification of cellulose occurs to a certain degree of conversion, depending on the amount of reactants applied and the reaction conditions, and usually the chemical modification is not complete so that the cellulose remains in a solid form as fibrils and does not dissolve in water. In physical modification, anionic, cationic or non-ionic substances or any combination of these are physically adsorbed on the cellulose surface.

[0138] The cellulose in the fibers may be especially ionically charged after said modification. The ionic charge of the cellulose weakens the internal bonds of the fibers and facilitates their subsequent disintegration into nanofibril cellulose. The ionic charge may be achieved by chemical or physical modification of the cellulose. The fibers may have a higher anionic or cationic charge after said modification compared to the starting material. The most commonly used chemical modification methods to create anionic charges are oxidation, sulfonation, and carboxymethylation, in which hydroxyl groups are oxidized to aldehydes and carboxyl groups. Chemical modification introducing groups such as carboxyl groups, which may participate in the formation of covalent bonds between nanofibril cellulose and bioactive molecules, may be desired. On the other hand, cationic charges may be chemically created by cationization by attaching cationic groups such as quaternary ammonium groups to the cellulose.

[0139] The nanofibril cellulose may be or may include chemically modified nanofibril cellulose, where the chemically modified nanofibril cellulose is, for example, anionically modified nanofibril cellulose or cationically modified nanofibril cellulose. In one example, the nanofibril cellulose is anionically modified nanofibril cellulose. In one example, the anionically modified nanofibril cellulose is oxidized nanofibril cellulose. In one example, the anionically modified nanofibril cellulose is sulfonated nanofibril cellulose. In one example, the anionically modified nanofibril cellulose is carboxymethylated nanofibril cellulose. Materials obtained by anionic modification of cellulose may be referred to as anionic cellulose, which refers to a material in which the amount or proportion of anionic groups, such as carboxylic acid groups, is increased by modification compared to the unmodified material. Instead of or in addition to carboxylic acid groups, it is also possible to introduce other anionic groups, such as phosphate or sulfate groups, into the cellulose. The content of these groups may be within the same ranges as disclosed herein for the carboxylic acids.

[0140] The cellulose may be oxidized. In the oxidation of cellulose, the primary hydroxyl groups of cellulose may be catalytically oxidized by heterocyclic nitroxyl compounds, such as 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical, commonly referred to as "TEMPO", such as N-oxyl mediated catalytic oxidation. The primary hydroxyl groups (C6-hydroxyl groups) of cellulosic β-D-glucopyranose units are selectively oxidized to carboxylic acid groups. Some aldehyde groups are also formed from the primary hydroxyl groups. In connection with the finding that a low degree of oxidation does not allow sufficiently efficient fibrillation, and that a higher degree of oxidation leads to degradation of the cellulose after mechanical disruption treatment, the cellulose may be oxidized until the carboxylic acid content in the oxidized cellulose, determined by conductometric titration, is at a level in the range of 0.5 to 2.0 mmol COOH / g pulp, 0.6 to 1.4 mmol COOH / g pulp, or 0.8 to 1.2 mmol COOH / g pulp, preferably 1.0 to 1.2 mmol COOH / g pulp. The fibers of oxidized cellulose thus obtained, when disintegrated in water, give a stable and transparent dispersion of individualized cellulose fibrils, the width of which may be, for example, 3 to 5 nm. Using the oxidized pulp as starting medium, it is possible to obtain nanofibrillated cellulose having a Brookfield viscosity, measured at a concentration of 0.8% (w / w), of at least 10000 mPa·s, for example in the range of 10000 to 30000 mPa·s.

[0141] Whenever the term catalytic "TEMPO" is mentioned in this disclosure, it is clear that all measurements and procedures involving "TEMPO" apply equally and similarly to any derivative of TEMPO, or any heterocyclic nitroxyl radical capable of selectively catalyzing the oxidation of the hydroxyl group at the C6 carbon in cellulose.

[0142] In one example, the chemically modified nanofibril cellulose, when dispersed in water, has a Brookfield viscosity of at least 10,000 mPa·s when measured at 20° C.±1° C. at a concentration of 0.8% (w / w) and 10 rpm. In one example, the chemically modified nanofibril cellulose, when dispersed in water, has a Brookfield viscosity of at least 15,000 mPa·s when measured at 20° C.±1° C. at a concentration of 0.8% (w / w) and 10 rpm. In one example, the chemically modified nanofibril cellulose, when dispersed in water, has a Brookfield viscosity of at least 18,000 mPa·s when measured at 20° C.±1° C. at a concentration of 0.8% (w / w) and 10 rpm. Examples of anionic nanofibril cellulose used have, depending on the degree of fibrillation, a Brookfield viscosity in the range of 13000-15000 mPa·s or 18000-20000 mPa·s or even a Brookfield viscosity of up to 25000 mPa·s.

[0143] In one example, the nanofibril cellulose is TEMPO oxidized nanofibril cellulose, which provides high viscosity at low concentrations, for example a Brookfield viscosity of at least 20000 mPa·s, even at least 25000 mPa·s, when measured at 20°C±1°C at a concentration of 0.8% (w / w) and 10 rpm. In one example, the Brookfield viscosity of the TEMPO oxidized nanofibril cellulose is in the range of 20000-30000 mPa·s, for example 25000-30000 mPa·s, when measured at 20°C±1°C at a concentration of 0.8% (w / w) and 10 rpm.

[0144] In one example, the nanofibril cellulose is or comprises chemically unmodified nanofibril cellulose, hi one example, such chemically unmodified nanofibril cellulose, when dispersed in water, at a concentration of 0.8% (w / w) and measured at 10 rpm at 20° C.±1° C., has a Brookfield viscosity of at least 2000 mPa·s, or at least 3000 mPa·s.

[0145] In one example, the nanofibril cellulose is or includes non-enzymatically modified nanofibril cellulose, which may be chemically modified or non-chemically modified nanofibril cellulose.

[0146] Auxiliaries, e.g. additives, may be included in the nanofibril cellulose dispersion to improve the manufacturing process or to improve or adjust the properties of the product. Such auxiliaries may be soluble in the liquid phase of the dispersion, may form emulsions or may be solid. They may be added to the raw materials already during the production of the nanofibril cellulose dispersion or to the formed nanofibril cellulose dispersion or gel. They may be added to the final product, e.g. by impregnation, spraying, dipping, soaking, etc. The auxiliaries are usually not covalently bound to the nanofibril cellulose and may therefore be releasable from the nanocellulose matrix. Controlled and / or sustained release of such agents may be obtained when using NFC as a matrix. Examples of auxiliaries include therapeutic (pharmaceutical) agents and other agents that affect the properties of the product or the properties of the active agent, such as buffers, surfactants, plasticizers, emulsifiers, etc. In one example, the dispersion contains one or more salts, which may be added to improve the properties of the final product or to facilitate the removal of water from the product during the manufacturing process. Examples of salts include chloride salts such as sodium chloride, calcium chloride and potassium chloride. The salts may be present in an amount ranging from 0.01 to 1.0% (w / w) based on the dry matter in the dispersion. The final product may also be dipped or soaked in a solution of sodium chloride, such as an aqueous solution of about 0.9% sodium chloride. The desired salt content in the final product may be in the range of 0.5 to 1% of the volume of the wet product, for example about 0.9%. The salts, buffers, and other agents may be provided to obtain physiological conditions.

[0147] Polyvalent cations may be included to obtain non-covalent crosslinking of the nanofibril cellulose. In one example, a nanofibril cellulose product is provided that comprises nanofibril cellulose (including in particular anionically modified nanofibril cellulose) and polyvalent cations (e.g. polyvalent metal cations selected from calcium, magnesium, zinc, aluminum, gold, platinum and titanium cations), in which the nanofibril cellulose is crosslinked by the polyvalent cations. The amount of the polyvalent cations may be in the range of 0.1-3% (w / w), e.g. 0.1-2% (w / w), calculated from the dry content of the hydrogel.

[0148] In one example, a method of making such a hydrogel is provided, the method comprising providing pulp, disintegrating the pulp until nanofibril cellulose is obtained, and forming the nanofibril cellulose into a hydrogel.

[0149] The nanofibrillated cellulose may be fibrillated to a desired degree of fibrillation, adjusted to a desired water content, or otherwise modified so that the nanofibrillated cellulose forms a gel having the desired properties described herein. In one example, the nanofibrillated cellulose in the hydrogel is anionically modified nanofibrillated cellulose.

[0150] The present application provides the use of nanofibril cellulose for making the medical products described herein.

[0151] Hydrogels used as medical or scientific hydrogels need to be homogenous. Thus, the method of making a hydrogel may include homogenizing the hydrogel comprising nanofibrillar cellulose, preferably by a homogenizing device such as that described herein. This preferably non-fibril-forming homogenization step makes it possible to remove areas of discontinuity from the gel. A homogenous gel is obtained with better properties for the application. The hydrogel may further be sterilized, for example by using heat and / or radiation and / or by adding sterilizing agents such as antimicrobials.

[0152] The starting concentration of the nanofibril cellulose dispersion (usually an aqueous dispersion) provided to treat the nonwoven in the soaking step may be in the range of 0.1-10%. Concentrated dispersions may be diluted before use. However, said concentration is usually below 5%, for example in the range of 0.3-5.0%, for example in the range of 0.8-1.2%. This is usually the initial concentration of nanofibril cellulose at the outlet of the manufacturing process, where the nanofibril cellulose is produced by disintegrating fibrous raw materials. However, it is also possible to dilute the nanofibril cellulose dispersion with a liquid from the initial concentration (product concentration from the manufacturing process) to a suitable starting concentration to ensure that the nanofibril cellulose dispersion is distributed or impregnated evenly in the nonwoven. Depending on the characteristic viscosity of the nanofibril cellulose grade, said starting concentration may be lower or higher and may be in the range of 0.1-10%. Higher concentrations may be used for low viscosity grades, which may spread evenly on the filter fabric despite the high concentration. Nanofibril cellulose originates as aqueous nanofibril cellulose from a manufacturing process in which fibrous starting material suspended in water is disintegrated. The drainage of liquid from a nanofibril cellulose dispersion can also be referred to as "dehydration" in the case of water or an aqueous solution.

[0153] Auxiliaries may be included in the nanofibril cellulose dispersion to improve the manufacturing process or to improve or adjust the properties of the product. Such auxiliaries may be soluble in the liquid phase of the dispersion, may form an emulsion, or may be solid. The auxiliaries may be added to the raw materials already during the production of the nanofibril cellulose dispersion, or may be added to the nanofibril cellulose dispersion before soaking. The auxiliaries may be added to the final product, for example by impregnation. Examples of auxiliaries include therapeutic and cosmetic agents, as well as other agents that affect the properties of the product or the properties of the active agent, such as surfactants, plasticizers, or emulsifiers. In one example, the dispersion contains one or more salts, which may be added to improve the properties of the final product or to facilitate the removal of water from the product during the manufacturing process. An example of such a salt is sodium chloride. The salt may be included in an amount ranging from 0.01 to 1.0% (w / w) based on the dry matter in the dispersion. The final product may also be dipped or soaked in a solution of sodium chloride, such as about 0.9% aqueous sodium chloride solution. The desired sodium chloride content in the final product may be in the range of 0.5-1% of the volume of the wet product, for example about 0.9%.

[0154] The cytotoxicity of the NFC wound dressing described herein was evaluated by utilizing the XTT test using mouse cells. The NFC wound dressing was extracted under stirring, and then the cells were incubated with various final concentrations of the extract. The highest extract concentration corresponds to a weight / volume ratio of 0.2 g / ml, as described in ISO 10993-5 and 10993-12. The extraction procedure did not reveal any abnormalities in the extraction medium or the test items. The extraction medium did not undergo any changes in terms of clarity, color, and the presence of foreign matter. The pH value of the test extract was 7.5 (solvent control pH 7.5).

[0155] The results showed no relevant decrease in cell proliferation and / or cell viability. At the highest extract concentration (100%), the dehydrogenase activity was not decreased. Microscopically, no inhibition of cell growth and no cell lysis was observed at all extract concentrations used. Controls confirmed the validity of the study. No relevant differences could be observed between the solvent control and the negative control. The positive control reduced the dehydrogenase activity to 1%, thus showing a clear decrease in cell viability and cell proliferation.

[0156] Medical Product Use The medical product may be used in several applications. One specific field is medical applications, where the material is applied onto living tissue such as skin. Structures such as patches, dressings, bandages, filters, etc. may be used in the medical product. The medical product may be a therapeutic product, such as a therapeutic patch containing a medicine. In general, the surface of the product, which comprises nanofibril cellulose, will be in contact with the skin during use. The surface of nanofibril cellulose may exhibit beneficial effects when in direct contact with the skin, for example, it may promote healing of wounds or other damage on the skin, or it may promote the transfer or delivery of substances from the skin to the medical product and / or from the medical product to the skin.

[0157] The term "wound" as used herein refers to any damage, injury, disease, disorder, etc. in tissues such as the skin, including open or closed wounds, where wound healing is desired and may be promoted by the products described herein. Wounds may be clean, contaminated, infected, or colonized, and, particularly in the latter case, may be administered with a therapeutic agent such as an antibacterial agent. Examples of open wounds include abrasions, avulsions, incisions, lacerations, punctures, and penetration wounds. Examples of closed wounds include burns, hematomas, crush injuries, suture wounds, grafts, and any applicable skin condition, disease, or disorder. Examples of skin conditions, diseases or disorders include acne, infections, vesicular diseases, cold sores, cutaneous candidiasis, cellulitis, dermatitis and eczema, herpes, hives, lupus, papular-scaling diseases, urticaria and erythema, psoriasis, rosacea, radiation-related disorders, pigmentation, mucinosis, ulcers such as pressure ulcers, atrophy, and necrobiosis, vasculitis, leukoplakia, warts, neutrophilic and eosinophilic disorders, congenital neoplasms and cancers such as melanomas and tumors of the epidermis or dermis, or other diseases or disorders of the epidermis and dermis such as complete or partial destruction of the dermis.

[0158] According to one example, said medical product is provided for use to treat and / or cover a skin wound or other injury. According to one example, such a medical product is provided for use as or in a dressing or patch to treat and / or cover a skin wound or other injury.

[0159] According to one example, such a medical product is provided for use to treat and / or cover a skin wound that is covered by a graft, such as a skin graft. According to one example, such a medical product is provided for use as or in a dressing or patch, for treating and / or covering a skin wound that is covered by a graft, such as a skin graft.

[0160] The medical product may be used in a variety of treatment methods, including providing the medical product and treating a subject with the medical product, e.g., applying the medical product onto the subject. The subject may be a patient, such as a human patient or an animal patient. In one example, the method includes: - Recognizing subjects in need of treatment requiring wound healing; - Providing said medical product; and - applying the medical product onto the wound of the subject; Includes.

[0161] Similarly, the medical product may be used to treat any other suitable disease or disorder of the skin or underlying tissue, such as the epidermis, in a subject. - Recognizing a subject in need of treatment that requires skin healing; - Providing said medical product; and - applying said medical product onto the skin of said subject; Includes.

[0162] The medical product may be provided for use to treat and / or cover skin wounds or other injuries or wounds, such as deep skin wounds including dermal injuries.

[0163] The present disclosure provides a method of treating a skin wound or other injury or wound, comprising applying a medical product as described herein onto the wound, injury, or wound. In one embodiment, a method of treating a skin wound covered by a graft, such as a skin graft (e.g., a mesh graft or a full thickness skin graft), is provided, comprising applying a medical product as described herein onto the graft.

[0164] Transplantation refers to a surgical procedure that transfers tissue from one part of the body to another, or from another person, without bringing its own blood supply with it. Instead, a new blood supply grows after the tissue is laid down. Autografts and syngeneic grafts are not usually considered foreign and therefore do not cause rejection. Allografts and xenografts are recognized as foreign and rejected by the recipient.

[0165] Skin grafts are often used to treat skin loss due to wounds, burns, infection, or surgery. In the case of damaged skin, the skin is removed and new skin is grafted in its place. Skin grafts can reduce the course of procedures and hospital stays required and may also improve function and appearance. There are two types of skin grafts: split-thickness skin grafts (epidermis plus part of the dermis) and full-thickness skin grafts (full thickness of epidermis plus dermis).

[0166] Mesh grafts are full or split thickness skin sheets that are fenestrated to allow for drainage and expansion. Mesh grafts are useful in many locations in the body because they conform to non-smooth surfaces. Mesh grafts can be placed in areas with excessive movement because they can be sutured to the underlying wound bed. Additionally, these fenestrations provide an outlet for bodily fluids that may accumulate underneath the graft, which helps reduce tension and risk of infection, as well as improve vascularization of the graft.

[0167] In clinical trials, it was found that the medical product adheres to the graft area and acts as a protective layer. As the graft heals, the product forms a scab-like structure with the graft. The properties of the product, including nanofibrillated cellulose, promote healing, and the medical product, along with the dry scab that forms, loosens in the same way that a normal scab behaves in the normal wound healing process. This reduces scarring of the wound and / or produces a better quality scar.

[0168] The treatment may require skin graft donor site management, which is a particular concern for elderly patients and those with poor wound healing abilities, as the donor site may also be a source of pain and discomfort. The NFC dressing, due to its single use, serves as an effective wound dressing in donor site care, as it does not require dressing replacement, which may also reduce the subjective pain experienced by the patient. The same is true for the treatment of other conditions.

[0169] The medical product may be provided for use to administer a bioactive or therapeutic agent or substance.

[0170] The medical product may be provided for use to treat a skin wound or other injury by a method comprising applying the product onto the wound to absorb a bioactive agent from the wound, storing the bioactive agent in the product for a period of time, and allowing the bioactive agent to diffuse back into the wound or other injury in a later phase of the wound or injury healing process.

[0171] One embodiment provides a medical product, such as a dressing, patch or filter, that includes the medical product described herein.

[0172] Dressings are sterile pads or compresses that are applied to wounds to promote healing and / or prevent further damage. Dressings are designed to be in direct contact with the wound, unlike bandages, which are mostly used to hold the dressing in place. Some organizations classify them as the same (e.g., the British Pharmacopoeia), and the terms are used interchangeably by some people. Dressings are frequently used in first aid and nursing.

[0173] An example provides the medical product for use in administering a therapeutic agent. In such cases, the medical product may be provided as is or, for example, in the form of a patch. The products described herein may include, for example be impregnated with, one or more therapeutic agents, and may be administered to a patient dermal or transdermal.

[0174] A medical product comprising a therapeutic agent may be provided, where the nonwoven and / or the coating layer comprising nanofibril cellulose comprises one or more therapeutic agents, such as a medicament or drug. The term pharmaceutical agent may also be used interchangeably in place of the term therapeutic agent. Such agents are active or effective agents, and are usually present in an effective amount. Such agents may be applied in a predetermined amount, for example an amount configured to provide a desired dose of the agent for a certain period of time, and / or an amount configured to provide a desired effect on a target, such as a wound, skin or other tissue. The content of the therapeutic agent in the product may be, for example, in the range of 0.1-5%. In particular, when the therapeutic agent is included, it may provide a sustained or prolonged release of the agent. In such cases, the nanofibril cellulose may contain a moisture portion to allow permeability of the agent. The moisture content of the product comprising a therapeutic agent may be in the range of 0-10%, for example in the range of 5-7%. The therapeutic agent may be present in water-soluble form, lipid-soluble form, or as an emulsion, or in another suitable form.

[0175] Examples of therapeutic agents that can be administered by using the medical products described herein include antibacterial agents, painkillers such as lidocaine; nicotine; opioids such as fentanyl or buprenorphine; hormones such as estrogen, contraceptives, or testosterone; nitroglycerin; scopolamine; clonidine; antidepressants such as selegiline; ADHD drugs such as methylphenidate; vitamins such as B12 or cyanocobalamin; 5-hydroxytryptophan; Alzheimer's drugs such as rivastigmine; acne drugs; psoriasis drugs, glucocorticoids such as hydrocortisone; or any other drug for treating skin diseases or disorders.The therapeutic agent can be used, for example, in a medical patch, which can be used on healthy or damaged skin to provide long-term, sustained, or extended release of the therapeutic agent from the patch, for example, over a period of several hours, for example, 6 hours or less, 12 hours or less, 24 hours or less, or even 48 hours or less.

[0176] According to one embodiment, the medical product is provided comprising an antimicrobial agent. Such a product is particularly suitable for treating wounds, in which wound treatment properties are combined with antimicrobial properties that prevent infection caused by harmful microorganisms in the wound. Examples of suitable antimicrobial agents include, in particular, topical antimicrobial agents such as bacitracin, erythromycin, clindamycin, gentamicin, neomycin, polymyxin, mupirocin, tetracycline, meclocycline, sulfacetamide (sodium), benzoyl peroxide, and azelaic acid, and combinations thereof. Other types of antibacterial agents may also be provided, such as systemic antibacterial agents, for example, penicillins such as phenoxymethylpenicillin, flucloxacillin, and amoxicillin; cephalosporins such as cefaclor, cefadroxil, and cephalexin; tetracyclines such as tetracycline, doxycycline, and lymecycline; aminoglycosides such as gentamicin and tobramycin; macrolides such as erythromycin, azithromycin, and clarithromycin; clindamycin; sulfonamides and trimethoprim; metronidazole and tinidazole; quinolones such as ciprofloxan, levofloxan, and norfloxacin.

[0177] Antibacterial agents may be used to treat acne, such as clindamycin, erythromycin, doxycycline, tetracycline, etc. Other agents may also be used, such as benzoyl peroxide, salicylic acid, tretinoin, topical retinoid drugs such as adapalene or tazarotene, azelaic acid, or androgen inhibitors such as spirolactone. Psoriasis may be treated with steroids, such as corticosteroids, moisturizers, calcipotriene, coal tar, vitamin D, retinoids, tazarotene, anthralin, salicylic acid, methotrexate, or cyclosporine. Insect bites or poison ivy exposure may be treated with agents such as hydrocortisone, emu oil, almond oil, ammonia, bisabolol, papain, diphenylhydramine, Impatiens moniliforme extract, or calamine. Some of these or other treatments may also be classified as cosmetic agents.

[0178] According to one example, a cosmetic product is provided, such as a dressing, mask or patch, which includes the medical product. Such a product may also be referred to as a cosmetic product. The product may be provided in various shapes, for example a mask may be designed to fit on the face, for example under the eyes, or on the chin, nose or forehead. As an example, the medical product is provided for use as a cosmetic product. The product may be used to deliver one or more cosmetic agents to a user, for example to the user's skin. Such a cosmetic product may include one or more cosmetic agents. One or more cosmetic agents may be included in, for example impregnated into, a product from which the cosmetic agent is released or delivered. The content of the cosmetic agent in the product may be, for example, in the range of 0.1-5%. The cosmetic agent may be present or provided in the product in a similar manner as described above for the therapeutic agent, and vice versa. The cosmetic use may be similar to the medical use described herein, in particular the administration of the therapeutic agent. The cosmetic agent may be used to cosmetically treat skin diseases or disorders such as those mentioned herein. Such cosmetic products may be used to treat, for example, pimples, acne skin, age spots, wrinkles, oily skin, dry skin, aging skin, spider veins, sun spots, dark circles under the eyes, etc. Examples of cosmetic patches include skin cleansers such as pore cleansers, blackhead removers, stretch stripes, short-term patch-like masks, short-term treatment patches, and night treatment patches.

[0179] Examples of cosmetic agents include various forms of vitamins and their precursors, such as vitamin A; retinoids, such as retinaldehyde (retinal), retinoic acid, retinyl palmitate and retinyl retinoate; alpha-hydroxy acids, such as ascorbic acid, glycolic acid and lactic acid; glycols; biotechnology products; keratolytic agents; amino acids; antimicrobial agents; moisturizers; pigments; antioxidants; plant extracts; cleansing or make-up removers; anti-cellulite agents, such as caffeine, carnitine, ginkgo and horse chestnut; conditioners; fragrances, such as aromatherapy agents and perfumes; urea, hyaluronic acid, lactic acid and glyceryl stearate. Moisturizing agents such as linoleic acid; emollients such as lanolin, triglycerides and fatty acid esters; ascorbic acid (vitamin C), glutathione, tocopherol (vitamin E), carotenoids, coenzyme Q10, bilirubin, lipoic acid, uric acid, enzyme mimetics, idebenone, polyphenols, selenium, spin traps such as phenylbutylnitrone (PBN), protein methionine groups, FR scavengers, singlet oxygen scavengers, superoxide scavengers or hydrogen peroxide scavengers such as superoxide dismutase, catalase, selenium peroxidase, heme oxygenase, or combinations thereof. The cosmetic agent may be present in a water-soluble form, a fat-soluble form, or an emulsion, or any other suitable form.

[0180] In one example, a method of cosmetically treating the skin is provided, the method comprising applying onto the skin the medical product described herein.

[0181] The term "patch" as used herein refers to a medical or cosmetic product that can be applied onto the skin. Examples of patches include dermal patches and transdermal patches. A dermal patch or dermal patch is a drug-containing adhesive patch that is placed on the skin to deliver a drug to the skin. A transdermal patch is a drug-containing adhesive patch that is applied to the skin to deliver a specific dose of a drug through the skin to the bloodstream. In one example, this promotes healing of an injured area of ​​the body. A patch may include a release liner that protects the patch during storage and is removed before use, and / or an adhesive to adhere the patch to the skin, and / or a backing material to protect the patch from the external environment. Examples of release liners include paper-based liners such as glassine paper, high-density kraft supercalender paper, clay-coated paper, silicone-coated paper, and polyolefin-coated paper; plastic-based liners such as polystyrene, polyester, polyethylene, unstretched polypropylene, and polyvinyl chloride; and composite liners based on a combination of several films. The adhesive layer may, for example, contain a pressure-sensitive adhesive (PSA).

[0182] Before applying the medical product onto the skin, the product may generally be pretreated, i.e. moisturized or moistened, with an aqueous solution. Moisturizing or moistening may be done, for example, by using water or saline (usually a NaCl solution of about 0.90% w / w, with an osmolality of about 308 mOsm / l). Other types of aqueous solutions, such as saline solutions with different concentrations, may also be used. Moisturizing or moistening the material improves contact with the skin and the formability of a sheet of material. EXAMPLES

[0183] Example 1 18051 Efficacy of wound dressings containing NFC-impregnated nonwovens in an ex vivo model background Ex vivo skin microdialysis An ex vivo human skin microdialysis method was developed to characterize the inflammatory response in human skin by sampling high molecular weight biomarkers such as cytokines from the extracellular compartment.

[0184] Healthy human skin was obtained following surgical removal of abdominal skin for cosmetic reasons.

[0185] Biomarker Panels The biomarkers measured were all selected for their relevance to the wound healing process: Interleukin (IL)-1α: IL-1α is a cytokine produced by neutrophils, monocytes, macrophages and keratinocytes. Skin injury due to disruption of the epidermal barrier mediates the immediate release of prestored IL-1α from keratinocytes, which is one of the first damage signals released to warn surrounding cells. This attracts neutrophils to the wound site to remove bacteria and increases keratinocyte migration and proliferation in an autocrine manner.

[0186] IL-1α is upregulated along with other proinflammatory cytokines during the inflammatory phase of wound healing and is known to be one of the major inducers of chemokines (along with TNF-α) within hours after wounding, thus acting to amplify inflammatory mediators.

[0187] IL-1α may have a protective function since it strengthens the epidermal barrier by influencing the mechanical adhesion of cells, but like any other mediator, it requires tight regulation since unregulated expression may promote an inflammatory skin phenotype, for example by attracting inflammatory cells.

[0188] IL-6: The cytokine IL-6 is produced by monocytes, macrophages, fibroblasts, endothelial cells, T cells, keratinocytes (the main source in the skin) and neutrophils. IL-6 is important for the initiation of the healing process as it is involved in the differentiation and growth of many cell types, exerting a key function in the proliferative phase of wound healing. This biomarker has been found to persist even in old wounds. IL-6 exerts a chemoattractant effect on neutrophils and macrophages, indirectly inducing collagen deposition, angiogenesis, epidermal cell proliferation by inducing TGF-β1, IL-1 and VEGF production (the latter promoting angiogenesis). In addition, IL-6 mediates keratinocyte migration and proliferation. Mice lacking IL-6 show delayed healing due to poor formation of granulation tissue and reduced re-epithelialization, angiogenesis, macrophage / neutrophil infiltration and matrix remodeling. It has been described that disrupted cutaneous barrier obstruction prevents normal induction of IL-6 expression.

[0189] IL-17: In skin wounds, the cytokine IL-17 is mainly produced by dermal gamma delta T cells, dendritic epidermal T cells and macrophages rather than Th17 cells. The IL-17 receptor is expressed on many cell types, including fibroblasts, keratinocytes and other inflammatory cells found in the skin. IL-17 is involved in many autoimmune diseases and is known as an important mediator in psoriasis, and is produced in large amounts when gamma delta T cells are stimulated by IL-23, which causes the infiltration of inflammatory cells. Conflicting results have been reported about the role of IL-17 in wound healing. IL-17 knockout mice with clean wounds covered by occlusive dressings showed accelerated wound closure, reduced neutrophil accumulation, increased myofibroblast differentiation and collagen deposition when compared to wild-type mice. It was also found that inhibition of IL-17 by blocking antibodies during the early inflammatory phase also accelerated wound healing. This indicates that the negative effect of IL-17 on the healing process and the excessive neutrophilic inflammation mediated by this cytokine are associated with impaired wound healing. However, when wounds are left open in IL-17 knockout mice, they show delayed healing, which may indicate that microbes are able to enter the wound and, due to the lack of IL-17 in the knockout mice, they counter the healing process to a greater extent in the wound.

[0190] Tumor necrosis factor (TNF)-α: TNF-α is a proinflammatory cytokine produced by various cell types, such as neutrophils, macrophages, keratinocytes, mast cells, and T cells. The effect of TNF-α on wound healing is highly duration- and dose-dependent, as low TNF-α levels have been shown to indirectly mediate inflammation and promote wound healing by stimulating macrophages to produce growth factors. However, high concentrations have a very negative effect on the healing process, especially when TNF-α is present for longer periods. TNF-α is expressed primarily by neutrophils within the first few hours after wounding and serves as the main inducer of chemokines. During the later phase, TNF-α is also expressed by macrophages. TNF-α acts synergistically with other inflammatory mediators, but TNF-α alone or at high concentrations has been shown to reduce re-epithelialization, thereby preventing restoration of the skin barrier. In contrast, application of TNF-α to mice after skin barrier disruption has been shown to enhance repair, and mice lacking the TNF-α receptor show delayed permeability barrier repair.TNF-α has been shown to suppress TGF-β-induced extracellular matrix production, and elevated levels of TNF-α have been observed in chronic non-healing wounds.

[0191] Epidermal growth factor (EGF): EGF is a growth factor secreted by platelets, fibroblasts and macrophages. After skin injury, EGF is released from degranulated platelets during hemostasis together with other mediators such as PDGF and TGF-β. The level of EGF in wounds is regulated by the proteolytic environment. EGF acts in a paracrine manner on keratinocytes, acting as an important mitogen and also promoting keratinocyte migration and thus re-epithelialization. The positive effects of EGF have been seen in clinical trials, where topical application of EGF to chronic wounds was found to shorten healing time and promote epithelialization.

[0192] Interferon (IFN)-y: The cytokine IFN-γ appears to be involved in the maintenance of the skin barrier by regulating the IL-4 and IL-31 receptors, which play a crucial role in the formation of the skin barrier by influencing cytokine signaling. IFN-γ also influences the detachment of keratinocytes from the basement membrane, which is an essential step in wound healing, as it is part of the differentiation process from basal to primary differentiated suprabasal cells. The lipid envelope, whose main function is to prevent transepidermal water loss (TEWL) and solute loss, is also influenced by IFN-γ, because this cytokine is involved in the synthesis of ceramide, which allows IFN-γ to participate in the regulation of TEWL.

[0193] Macrophage migration inhibitory factor (MIF): MIF is an inflammatory cytokine that is widely expressed in skin, but is localized in endothelial cells, epidermis, melanocytes, and cells of sebaceous and eccrine sweat glands, and is highly expressed in keratinocytes. MIF stimulates the production of several other cytokines and is strongly induced during skin wound healing, but the induction is mainly by inflammatory cells during the early phase. In the later phase, fibroblasts have been shown to upregulate MIF expression. The effect of MIF on wound healing is controversial, as some studies claim a negative effect. However, disruption of the Mif gene does not promote or inhibit incisional wound healing in mice, but co-treatment of Mif null mice with recombinant MIF actually impairs incisional wound healing. MIF is involved in various skin diseases, and polymorphisms in the Mif gene that lead to increased expression are associated with increased disease severity in more inflammatory diseases. Therefore, there is no consensus regarding its role in cutaneous wound healing, although various studies indicate that MIF may impair normal repair.

[0194] Neutrophil-activating peptide (NAP)-2: NAP-2 (also known as CXCL7) is a CXC chemokine that is released by activated platelets as chemokine-binding tissue-activating peptide-III (CTAP-III), which is proteolytically converted by neutrophils to neutrophil-activating peptide-2 (NAP-2). NAP-2 acts as a first-line mediator within minutes of injury, mediating a chemotactic effect on neutrophils through the CXCR2 receptor, in addition to inducing endothelial cell proliferation and angiogenesis.

[0195] Materials, methods and experimental design Test items NFC coating material A medical product in the form of a wound dressing was made from nanofibril cellulose obtained from bleached birch pulp and a polyester-viscose based nonwoven. The medical product according to the embodiment is hereinafter referred to as NFC dressing.

[0196] NFC coating material Reference product Name: Suprathel(R) wound dressing Product: A wound dressing product consisting of a synthetic copolymer consisting primarily of DL-lactide (>70%), trimethylene carbonate, and e-caprolactone. Intended therapeutic use: shallow wounds after skin grafts Batch number: P-2014-X / P-2016-XX Appearance: 9×10cm white covering material Expiration date: 2017-7 / 2019-12 Supplier:Polymedics lnnovations GmbH, Germany

[0197] Experimental study design According to the protocol, the study was divided into two phases: 1) a pre-study phase and 2) a main study phase. Pre-test phase: First, the relative biomarker recovery was established for each biomarker in the panel. Furthermore, a standardized ex vivo skin wound model induced by skin barrier disruption was established using a pre-phase. This was achieved by analyzing the biomarker profile using microdialysis sampling at three different time points after the initial skin trauma. Both general and cell-specific inflammatory biomarkers were investigated.

[0198] Primary Study Phase: This part of the study aimed to investigate how NFC interacts with the wound healing process in an ex vivo skin model established in the pre-phase. This was done in three separate studies:

[0199] M1. Ex vivo skin microdialysis with and without skin barrier disruption with NFC wound dressing versus market leader (Suprathel®) wound dressing material. Read-out: Biomarker profile in the wound bed.

[0200] M2. Analysis of NFC dressings after application on tape-stripped ex vivo skin. Possible cell migration into the hydrogel layer and cytokine content of the cellulose layer are analyzed. Readouts: cell number and biomarker profile.

[0201] M3. *** Keratinocyte cell lines were grown through microdialysis dialysate sampled after tape stripping and NFC application to investigate the potential effects of NFC on keratinocyte proliferation and re-epithelialization. Readout: Daily keratinocyte growth rate over 5 days.

[0202] Donor skin Abdominal skin samples were obtained from patients undergoing cosmetic surgery (e.g., due to the presence of excess skin after pregnancy or weight loss). The skin was transported to the RefLab at room temperature in a box provided with sterile saline immediately after surgical removal.

[0203] With ethical approval from the Danish National Committee on Health Research Ethics according to the Committee's Regulation § 14, section 3 on the use of de-identified human material for research purposes, the skin was fully de-identified and therefore obtained and used in this study.

[0204] Estimation of relative biomarker recovery Relative biomarker recovery was determined as part of a pre-phase study using a self-developed "skin reservoir model." Here, a known amount of each biomarker was injected into thawed skin, which served as a reference solution reservoir. The biomarkers were then sampled through an inserted microdialysis probe (3000 kDa molecular weight cutoff) at a flow rate of 0.8 μL / min for 2 hours (see procedure and detailed description of probe insertion for microdialysis sampling in section "4.7 Microdialysis technique"). Biomarker concentrations were then measured in the dialysate using a commercially available ELISA kit to estimate relative recovery (see section "4.8 Analysis of biomarker profile in microdialysate").

[0205] Relative recovery is defined as the biomarker concentration found in the dialysate divided by the injected concentration, and is therefore the fraction of the biomarker that crosses the membrane: Concentration 透析液 / concentration 注入 ×100%.

[0206] Preparation of the skin and induction of trauma Approximately 4x6cm (for pre-phase experiments) or 4x3cm (for main phase experiments) pieces were excised from abdominal skin according to the respective set-up (see below). After trimming the subcutaneous fat, the skin samples were briefly washed in ethanol and antimycotic to ensure aseptic handling. In the LAF bench, the skin samples were attached to the styrofoam using a sterile needle, with moist tissue paper between the styrofoam and the skin sample. The skin samples were treated according to the set-up below, thereby subjecting them to injury, or kept as non-injured controls, and either had or did not have a dressing applied.

[0207] Deviation from signed protocol: The protocol states that "To stimulate split-thickness skin grafts, 3M cellophane tape is used to strip the skin slices 10-15 times." In the ex vivo skin model, it was not possible to induce injury by tape stripping method because the skin needs to be kept moist during the entire procedure, and tape does not adhere to moist skin. Experiments showed that injury could be induced by rubbing the skin with sandpaper (grain size 150) 15 times, so this procedure was followed throughout the study.

[0208] Pre-phase experimental setup The setup shown in FIG. 1 was repeated on skin from three donors, and six skin samples were generated from each donor (two at each of three different time points: 6 hours, 24 hours, and 48 hours).

[0209] Readouts: dialysate concentrations of IL-1α, IL-6, IL-17, TNF-α, EGF, IFN-γ, MIF and NAP-2.

[0210] Main Phase 1+2 Experiment Setup

[0211] The setup shown in FIG. 2 was repeated on skin from 5 donors, and from each donor 12 skin samples were generated (6 each for two different time points: 6 hours and 24 hours).

[0212] Microdialysis was performed after 6 and 24 h of incubation (M1, time point chosen based on pre-phase experiments).

[0213] NFC dressing strips incubated on skin samples B6 / B24 and E6 / E24 were enzymatically digested using GrowDase™ for 2 hours and then flushed with PBS to release biomarkers that may be bound to the hydrogel layer. Both the GrowDase™ fraction and the PBS flush were analyzed for biomarker content (M2).

[0214] The pellet from the GrowDase mixture and the PBS fraction from the NFC dressing pieces from donor 1 were examined for possible cell infiltration, but no cell infiltration was observed, so cell infiltration was not examined in further experiments.

[0215] Readouts: dialysate concentrations of IL-1α, IL-6, MIF, and NAP-2 (M1, biomarkers were selected based on pre-phase experiments) and levels of IL-1α, IL-6, IL-17, TNF-α, EGF, IFN-γ, MIF, and NAP-2 in NFC dressings degraded by GrowDase (M2).

[0216] microdialysis technology A microdialysis probe was placed intradermally in the skin sample using a 21G guide cannula, purchased from EP Medical (Copenhagen, Denmark), with a molecular weight cut-off of 3000 kDa and a total membrane length of 40 mm, which was attached to the inlet tubing.

[0217] In the pre-phase experiments, four probes were inserted into each skin sample, spaced 1 cm apart with an intradermal length of 20 mm, and in the main phase experiments, two probes were inserted into each skin sample (see schematic of the experimental setup above).

[0218] The prepared skin samples with inserted cannulae were placed on stainless steel grids placed in Petri dishes in 5% CO 2 The cells were incubated for up to 48 hours (according to the respective set-up) at 37°C in humidified air containing 0.1% ethanol, where below the grid was present RefLab developed skin medium containing nutrients and antibiotics (DMEM supplemented with 5% FCS, 2mM GlutaMAX, 1% penicillin / streptomycin / amphotericin and 5μg / ml recombinant human insulin) to mimic an air-liquid interface.

[0219] After incubation, the skin was peeled off from the metal grid and placed on moist tissue paper attached to a polystyrene foam at room temperature. The microdialysis probe was inserted through the guide cannula, which was withdrawn, leaving the probe inside the skin and ready for perfusion using a microperfusion pump (NE-1200-EM, Harvard Apparatus, World Precision Instruments, Hertfordshire, UK). The perfusion solution consisted of lactated Ringer's supplemented with 1% human albumin and 4 mM lactate. The perfusion rate was set at 0.8 μL / min throughout the study, and the dialysate was sampled continuously for 2 h into PCR tubes covered with parafilm to reduce evaporation. The volume of the dialysate was determined by weighing the PCR tubes before and after sampling. Immediately after weighing, the tubes were stored at -80°C until analysis to minimize possible degradation of labile biomarkers.

[0220] Analysis of biomarker profiles in microdialysates Dialysates (from prephase and M1) and samples from GrowDase degraded NFC dressings (from M2) were analyzed for biomarker concentrations by DuoSet ELISA kits commercially available from R&D, following the manufacturer's instructions. The biomarker panel consisted of: IL-1α, IL-6, IL-17, TNF-α, IFN-γ, EGF, NAP-2 and MIF. Dialysates from the same skin sample were pooled prior to ELISA analysis.

[0221] statistical analysis All statistical analyses were performed using GraphPad Prism version 7.0.3 (GraphPad Software Inc., La Jolla, CA, USA). The statistical tests used are described in the figure legends, and asterisks denote the significance level based on the p-values ​​obtained from the statistical tests: * =p<0.05, ** =p<0.01.

[0222] result Pre-phase results Relative recovery measured in a skin reservoir model The relative recovery, a measure of sampling feasibility, was estimated for each of the biomarkers in the biomarker panel (IL-1α, IL-6, IL-17, TNF-α, EGF, IFN-γ, MIF, and NAP-2) using the RefLab "skin reservoir model." The results are listed below and summarized in Table 1: 1. IL-1α, IL-6, IL-17, TNF-α, EGF and NAP-2 showed relative recoveries above the lower acceptable level, which is usually set at 5%. 2. The relative recovery of MIF could not be quantified because background levels in thawed skin exceeded the injected concentration and the dialysate concentration exceeded the upper limit of quantification (ULOQ), but this finding indicates that MIF can indeed be sampled by the microdialysis probe. 3. The relative recovery rate of IFN-γ was 0.0%, and IFN-γ could not be measured in the dialysate. Relative biomarker recovery

[0223] [Table 1]

[0224] Establishment of ex vivo wound model As part of the pre-phase (Figure 1), we quantified all eight biomarkers in dialysates from control and traumatized skin samples. We found that four of the eight biomarkers examined were detectable at concentrations above the lower limit of quantification (LLOQ):

[0225] IL-1α was highly upregulated by trauma in all three donors, with maximal upregulation after 6 hours. Basal levels appeared unchanged up to 48 hours (Figure 3). IL-6 was highly upregulated by trauma in all three donors after 6 hours. After 24 and 48 hours, the upregulation was accompanied by increasing basal levels (Figure 4). MIF was upregulated due to trauma in all three donor skin samples, but primarily after 6 and 24 hours. Basal levels were upregulated after 24 and 48 hours (Figure 5). High basal levels of NAP-2, ranging from 800 to 1300 pg / mL, were observed in all three donors, but were not further upregulated in the traumatized skin (Figure 6).

[0226] In contrast, EGF (Figure 7), IFN-γ (Figure 8), IL-17 (Figure 9), and TNF-α (Figure 10) were either absent or below the LLOQ in the dialysate. The fact that it was not possible to sample IFN-γ using the microdialysis probe (Table 1) explains the absence of this cytokine in the dialysate (Figure 8).

[0227] Main Phase 1 Based on the results from the pre-phase establishing a human ex vivo skin wound model, IL-1α, IL-6, MIF and NAP-2 were quantified in the dialysate after treatment with NFC dressings and Suprathel®. All eight biomarkers were quantified in samples from NFC dressings degraded by GrowDase™.

[0228] IL-1α levels in the dialysate Dialysate levels of IL-1α were observed to increase in response to injury (FIG. 11A; compare 6 hour time point without dressing with wounded vs. non-wounded). Without dressing treatment, IL-1α levels were observed to spontaneously decrease over time in wounded skin samples (FIG. 11A, compare undressed samples at 6 and 24 hours). Suprathel® mediated a relative increase in IL-1α compared to undressed controls, resulting in elevated IL-1α responses at both time points (FIGS. 11B and 11D, significant only at 24 hours). There was also a slight increase in IL-1α levels in non-wounded samples, but this was not statistically significant (FIG. 11C). NFC dressings, on the other hand, appear to mediate a slight decrease in IL-1α responses at early time points (highly variable between donors and not significant), but produce a slight (but not significant) upregulation at later time points (Figure 11D).

[0229] IL-6 levels in dialysate Dialysate levels of IL-6 were observed to increase in response to trauma (FIG. 12A; compare no dressing at 6 hours with wounded vs no wounded). Background levels of IL-6 (no dressing control samples) increased from 6 hours to 24 hours across all donors (FIG. 12A, compare no wound control at 6 and 24 hours). Suprathel® induced a decrease in IL-6 levels in wounded skin samples, observed at both time points across all donors, but significant only at 6 hours (FIGS. 12B and 12D, compare no dressing control with Suprathel®-treated skin samples). Furthermore, Suprathel® blocked the upregulation of IL-6 observed in the no wounded control samples, although this was not statistically significant (FIGS. 12B and 12C). NFC dressing treatment appeared to mediate a decrease in IL-6 when compared to no dressing controls (in traumatized skin samples), but not to the same extent as Suprathel®, and this trend was not statistically significant (Figures 12B and 12D).

[0230] MIF levels in dialysis fluid Dialysate levels of MIF are strongly upregulated in response to injury (FIG. 13A; compare uncoated control samples with and without injury at 6 and 24 hours, respectively). NFC coatings mediate a decrease in MIF at both early and late time points (FIGS. 13B and 13D, compare uncoated controls at 6 and 24 hours). Suprathel® may also reduce levels of MIF, but to a lesser extent than the NFC coating, and this is not significant when compared to uncoated controls (FIGS. 13B and 13D).

[0231] NAP-2 levels in dialysis fluid Background levels of NAP-2 appear to decrease over time (FIG. 14A, no coating control). NAP-2 levels show a large variation between donors. Incubation with Suprathel® for 6 hours mediated a significant decrease in NAP-2 levels (FIGS. 14B and 14D, comparing Suprathel® treatment with no coating control). On average, incubation with the NFC coating was observed to decrease levels of NAP-2 compared to the no coating control, but this was not significant due to the larger variability between donors.

[0232] 5.3: Main Phase 2 IL-1α levels in NFC dressing samples GrowDase™-facilitated degradation of the NFC dressing mediated the release of IL-1α from NFC dressings incubated on traumatized skin samples after both 6 and 24 hours of incubation (FIG. 15A). Low levels of IL-1α were detected in some NFC dressing strips incubated on non-traumatized control samples (FIG. 15A). IL-1α levels decreased over time in both the dialysate and the NFC dressing (FIGS. 15A and 15B). A statistically significant correlation was found between IL-1α levels in the dialysate and NFC dressing samples from traumatized skin samples after both 6 and 24 hours of incubation (FIG. 15C).

[0233] IL-6 levels in NFC dressing samples GrowDase™ treatment of NFC dressings mediated the release of IL-6 from strips incubated on traumatized skin samples after both 6 and 24 hours of incubation (FIG. 16A). Low levels of IL-6 were also observed in NFC dressings incubated on non-traumatized control samples for 24 hours. IL-6 levels were observed to increase over time in NFC dressings, but this trend was only seen in dialysates from non-traumatized control samples, not in dialysates from traumatized skin samples (FIGS. 16A and 16B). There appears to be a positive linear relationship between IL-6 levels in NFC dressing samples and IL-6 levels in dialysate samples, but this was not statistically significant by Pearson correlation (FIG. 16C).

[0234] MIF levels in NFC coating samples MIF was recovered from NFC dressings incubated on traumatized skin samples (Figure 17A), but not from NFC dressings incubated on non-traumatized control samples. This correlates with increased MIF levels in dialysates from traumatized skin samples compared to non-traumatized controls (Figure 17B). Dialysate levels of MIF decreased from 6 to 24 hours with trauma (Figure B), which was also seen in NFC dressing samples from 4 out of 5 donors (Figure 17A). MIF was present in dialysates from non-traumatized control skin samples, but MIF was not detected in the corresponding NFC dressing samples (Figures 17A and 17B). We found a positive linear relationship between MIF levels in NFC dressing samples and MIF levels in dialysate samples, which was not significant according to Pearson correlation performed for both time points (Figure 17C).

[0235] NAP-2 levels in NFC coating samples NAP-2 was recovered from NFC dressings incubated on wounded and non-wounded skin samples at both time points (FIG. 18A). Levels of NAP-2 in the NFC dressings were generally higher when incubated on wounded skin samples (FIG. 18A, except after 6 hours of incubation for donor 1). While there was a trend for dialysate levels of NAP-2 to decrease over time (FIG. 18B), NAP-2 levels in the NFC dressing samples appeared to either remain unchanged or increase over time, except for donor 1 (FIG. 18A). After 6 hours of incubation, a negative linear relationship was observed between NFC dressing levels and the corresponding dialysate levels, but after 24 hours, a positive linear relationship was observed. None of these correlations were statistically significant.

[0236] Consideration In the pre-phase, we found that seven of the eight biomarkers in the panel were successfully recovered by the microdialysis probe. Although we were unable to quantify the relative recovery of MIF due to the high background levels of cytokines in thawed skin, we could conclude that MIF easily passed through the microdialysis membrane. IFN-γ was the only biomarker not recovered in the skin reservoir model. However, this finding is consistent with the very low in vitro recovery of IFN-γ described in the literature (Ao and Stenken 2006).

[0237] As can be seen from the pre-phase results, only IL-1α, IL-6, MIF and NAP-2 were detectable biomarkers in viable skin, which is why they are the main focus of subsequent experiments.

[0238] In the main phase, we found that IL-1α was significantly upregulated after 24 hours in response to Suprathel® treatment, whereas IL-6 was significantly decreased after 6 hours of incubation with Suprathel®. NFC dressing mediated a similar trend for IL-6, but it was less pronounced and not statistically significant. Only MIF changed significantly in response to NFC dressing treatment, decreasing statistically significantly both at 6 and 24 hours. NAP-2 levels were significantly lower after 6 hours of incubation with Suprathel®.

[0239] Surprisingly, we found that the biomarkers measured in the dialysate were also detectable in the NFC dressing after degradation by GrowDase. This leads us to speculate that the NFC dressing may exert its effect on the wound healing process by acting as a biomarker reservoir. The reservoir may be a passive reservoir that only removes fluid and mediators from the tissue, or a functional / active reservoir in that it may facilitate two-way diffusion over time, uptake of active mediators from the tissue and delivery of active mediators to the tissue. It is possible that the biomarkers taken up by the NFC dressing are somewhat stabilized in the hydrogel layer and may therefore have a different turnover compared to the tissue environment.

[0240] As the biomarkers appear to have different diffusion and / or stability properties in tissue compared to the hydrogel layer of the NFC dressing, it is well known that individual molecules have different physiochemical properties, which is supported by the results: IL-1α was observed to be present in the NFC dressing incubated on the non-wounded control skin samples, whereas this cytokine was not detected in the corresponding dialysate. In contrast, MIF was absent in the NFC dressing incubated on the non-wounded skin, whereas it was observed in the corresponding dialysate.

[0241] The absence of IL-1α in dialysates from unwounded skin samples, despite its measurable presence in the corresponding NFC dressing samples, may indicate that tissue IL-1α is rapidly degraded, in contrast to IL-1α in the dressing, or that IL-1α is simply removed from the tissue through unidirectional diffusion into the dressing and retained within the dressing.

[0242] Regarding MIF, this cytokine may be more stable in the NFC dressing than in the tissue within the first 24 hours, since the levels in the tissue decreased over time, whereas the levels in the NFC dressing did not. It can be assumed that the NFC dressing is saturated with MIF after 6 hours, which would explain why the NFC dressing levels were similar at both measured time points. The absence of MIF in the NFC dressing incubated in the non-injured sample, despite the presence of measurable MIF in the dialysate, may indicate that diffusion of MIF into the NFC dressing requires disruption of the skin barrier.

[0243] The IL-6 levels in the NFC dressing increased over time, whereas the IL-6 levels in the dialysate seemed to remain constant and did not increase, indicating that IL-6 accumulates in the NFC dressing over time. Another possible explanation is that the diffusion of IL-6 into the NFC dressing is delayed, so that it does not reflect tissue concentrations at early time points. The turnover may also be different in the skin compared to the dressing, and there may be a lower degree of degradation in the dressing compared to the tissue.

[0244] No clear trends were observed regarding NAP-2 levels in the NFC coatings.

[0245] One of the main features of the NFC dressing is the formation of a hydrogel layer due to the NFC content in the dressing. This helps maintain a moist wound environment and removes excess exudate. In addition, the dressing itself acts as a physical barrier to the surroundings, thus preventing infection. In this sense, occlusion is beneficial and necessary for efficient wound healing, but as mentioned above, excessive occlusion can have a negative effect, for example, on IL-6 induction. Macroscopically, the NFC dressing appears to be less occlusive compared to Suprathel® when the two dressings were examined and handled in the experimental setup. This observation, combined with the fact that both Suprathel® and NFC dressings reduced IL-6 compared to the no dressing control, and that Suprathel® had a more pronounced effect, supports the hypothesis that the NFC dressing is less occlusive. The NFC dressing appears to facilitate a beneficial moist environment while allowing more efficient gas exchange, which may explain some of the positive effects of the NFC dressing on wound healing.

[0246] conclusion This study demonstrates how human ex vivo skin combined with microdialysis technology can be used as a preclinical model to investigate early stage cutaneous wound healing and the effects of different wound dressings, in this case NFC dressing and Suprathel®.

[0247] It was found that four of the eight biomarkers in the biomarker panel were efficiently sampled from traumatized skin samples, and several of these biomarkers were significantly affected by incubation of the skin with the dressing.

[0248] Only MIF levels were statistically significantly affected by the NFC dressing, as seen by the reduction in MIF levels after 6 and 24 hours of incubation with the NFC dressing, whereas Suprathel® was observed to mediate a statistically significant reduction in IL-6 and NAP-2 levels at 6 hours, and IL-1α was significantly upregulated at 24 hours in response to Suprathel® treatment.

[0249] Overall, there appears to be a positive relationship between the levels of cytokines recovered from the NFC coating and those measured in the dialysate, although this was only statistically significant for IL-1α. Nonetheless, the ability of the NFC coating to contain cytokines may be an important finding.

[0250] Example 2 It was found that the NFC dressing is capable of binding various soluble factors associated with the wound healing process and found in wound fluids. Therefore, the retention levels of TNFα, IL-10 and TGFβ1 in the NFC dressing matrix compared to media and NFC cloth (without NFC) were investigated. Purified growth factors that were demonstrated to be stable in culture for up to 4 hours were incubated with RPMI media, NFC dressing cloth and NFC dressing for 1 hour and 4 hours. Free cytokines were measured by ELISA and compared to the 1 hour media control. No significant degradation or loss of cytokines was observed in the media control over the 4 hour incubation period. The NFC dressing significantly reduced free TNFα levels compared to the cloth at 4 hours, indicating that the NFC component may be capable of retaining cytokines within the gel. The levels of IL-10 were significantly reduced by exposure to the cloth and NFC dressing at both 1 hour and 4 hours. The same effect was seen for TGFβ1 at 4 hours, with the highest effect consistently seen in the NFC dressing matrix compared to cloth alone or medium. Together, these data demonstrate that the NFC dressing is capable of absorbing active biomolecules from the wound environment.

[0251] Example 3 The results of this study indicate that NFC dressings are suitable products for wound care. The data demonstrate that NFC is a unique material utilized in the wound healing dressings described herein with properties that actively participate in enhancing the innate wound repair response. The NFC dressings have been shown to have the potential to reduce the likelihood of oxidative stress in surrounding immune cells that actively contribute to the wound healing process.

[0252] Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and incubated with NFC dressings + / - LPS for 24 hours while measuring reactive oxygen species (ROS) generation at multiple time points. The objectives of these studies were to establish (a) whether the NFC dressing matrix is ​​recognized as foreign and induces an oxidative stress response, and (b) whether the NFC dressing binds LPS and increases any baseline level of ROS generation by PBMCs. ROS generation was assessed using a fluorometric method to evaluate the conversion of H2DCFDA to the fluorescent compound DCF, which is cleaved by ROS to its fluorescent form. The data show that at the 1 hour time point, the basal level of oxidative stress in the RPMI controls was significantly higher than in the NFC dressing samples. Although not statistically significant, at multiple time points, both the NFC dressing and the NFC dressings + LPS showed lower ROS readouts than their respective controls. These data may indicate that the NFC coating protects cells from oxidative stress, possibly through binding of soluble factors released by PBMCs. It may be concluded that the NFC coating does not exert a stress response as a foreign body in PBMCs.

[0253] Example 4 In this example, the physical properties of the present NFC wound dressing are characterized and compared to properties measured on other development versions. In total, nine different standard test methods and internal methods were used to characterize the samples.

[0254] Materials and Methods sample The main motivation guiding the selection of samples was the intention to evaluate samples made by different methods or using slightly different raw material compositions. According to these criteria, six wound dressings and one untreated nonwoven were selected. These samples represent different NFC wound dressing development versions and the individual raw materials used in the production. All samples were steam sterilized according to a typical sterilization procedure (121.6°C; 20 min) before analysis. The nominal basis weight of nonwovens used in medical products is approximately 45 g / m 2 Basic sample information is presented in Table 2. Samples analyzed during the study

[0255] [Table 2]

[0256] Analysis method All methods selected for this study are presented in Table 3. Analytical methods used to characterize the physical properties of the samples

[0257] [Table 3]

[0258] When determining the free swell absorbent capacity of the samples, the method described by the EN13726-1 standard: "Test methods for primary wound dressings. Part 1: Aspects of absorbency" was followed. In brief, the method is based on weighing the samples before and after immersion in an excess of physiological test solution at a temperature of 37°C for 30 minutes. The absorbent capacity was then expressed in g / 100cm 2 Measurements were carried out under conditions specified by standard test method EN13726-1 using a physiological test solution containing 142 mmol sodium chloride and 2.5 mmol calcium chloride.

[0259] The liquid retention of the dressings was measured using an internal method based on the original method presented in Mennini, N., et al. "Quality of wound dressings: a first step in establishing shared criteria and objective procedures to evaluate their performance." Journal of wound care 25.8(2016):428-437. In this method, a free swell absorbent sample was sandwiched between two wire cloths and placed on a piece of absorbent paper. A plexiglass plate was then placed on top of the sample applying 40 mmHg of pressure. After 30 minutes, the weight was removed and the sample was weighed. Liquid retention was calculated based on the observed weight difference and reported as a percentage of liquid remaining.

[0260] The area changes of wound dressings when wet and dry were determined simultaneously with the free swell and liquid retention tests. These internal methods use the simple principle of measuring the dimensions of the wound dressing before and after the free swell test and once again after drying the sample overnight at 60°C in a laboratory oven. The area changes are reported as a percentage change compared to the original dried sample (e.g., -4% = 4% area shrinkage). When the observed area changes are small, the results should be considered approximate, as the dimensions (x and y) are measured using a ruler with limited accuracy. Also, the dimensional changes of the wound dressings can be difficult to measure accurately due to non-uniformity.

[0261] Moisture vapor transmission rate (MVTR) is a standard method in SFS-EN-13726-2 that measures the rate of water vapor that permeates a film-like wound dressing. In this method, the sample is placed on a flanged sample cup filled with purified water and secured in place by a lid. The lid is fitted with a 10 cm flange, which is the only path by which water vapor can evaporate through the wound dressing. 2The sample cup (+ the water and the sample) is weighed and placed in a climate chamber (37°C, RH% < 20) for 18-24 hours. Finally, the cup is reweighed and the MVTR is calculated based on the weight loss in g m 2 Calculated as / 24.

[0262] Deviations from standard methods For the determination of the free swell absorption capacity, due to limited sample material, only 5 replicates were measured instead of 10. There were no other deviations from the standard test method EN13726-1.

[0263] result The averaged results from this comparative study are summarized in Table 4. In the following sections, some selected characteristics and corresponding results are discussed in more detail. Pooled and averaged results for the samples studied

[0264] [Table 4]

[0265] Air permeability and basis weight The results for basis weight and air permeability, which are also used as standard quality control indicators, showed mostly expected results: both the machine-produced and the manually produced NFC wound dressing (Type 4) met the specifications set for the sterilized product (<1000 ml / min and 50–55 g / m 2 ). However, the manually produced NFC wound dressing had slightly higher basis weight and air permeability than the machine produced NFC wound dressing. Air permeability results are typically used indirectly to monitor the distribution and especially the uniformity of the NFC coating. Here, the lower air permeability result of the machine produced NFC wound dressing (67 ml / min) suggests that the uniformity of the NFC is at the same level or better than the manually produced NFC wound dressing (624 ml / min).

[0266] The other samples gave very theoretical values, since the higher basis weight values ​​in most cases implied a lower air permeability. The two extremes were samples representing the individual raw materials NFC and nonwoven, respectively. The NFC-film was practically impermeable, while the commercial nonwoven showed a value of 8820 ml / min, exceeding the maximum measurement range of the method. The LW-NFC coating (12-2018-AA) at 53.3 g / m 2 The higher basis weight result was 50.8 g / m2, measured previously in December 2018. 2 This was somewhat unexpected as it showed average basis weight values ​​of

[0267] Figure 22 shows a simple visual representation of the relationship between basis weight and air permeability using the data summarized in Table 2. The horizontal line indicates the upper specification limit for air permeability, and the curve is a rough polynomial fit to the data. Although the measured samples differed from each other in many ways, this polynomial fit gives some indication of the probability of reaching the air permeability target as the basis weight is increased. The data point for the LW-NFC coating is marked with a cross because it is an erroneously high basis weight value, but this is not excluded from the data here.

[0268] physical dimensions Fundamental dimensions such as bulk thickness and bulk density mostly showed expected values, but also some surprising behavior. The measured sample thicknesses are compared in Figure 23. The NFC film was found to be the thinnest and clearly had the highest density (924 kg / m) of all samples. 3 ). In terms of thickness, the machine-produced NFC-coated samples (NFC-coated, HW-NFC-coated, and LW-NFC-coated) were logically distributed according to the NFC content of each sample and were all thicker than the commercial nonwovens. However, the manually produced samples (NFC-coated type 4 and anionic NFC-coated) were thinner than the uncoated commercial nonwovens. It appears that the manual processing flattened the samples somewhat. This could be attributed to the nip pressure and the five thin coats applied during the manual production method.

[0269] The change in area of ​​the wound dressing was measured for all samples when wet and when dry after wetting. No significant differences were observed in the behavior of the dressing during the measurements. When wet, most samples swelled by approximately 1 mm in width, which corresponds to an area change of about +2%. However, the NFC-film stood out from the other samples, as it changed in area by +5.7%. When the samples were dried, all samples shrunk to at least their original dimensions or slightly smaller. It should be noted that an area change of less than 2% is small, so the method is not accurate enough to reliably quantify the area change. Therefore, most of these results are only approximate.

[0270] Liquid Handling Properties Liquid retention describes the ability of a sample to retain absorbed liquid under an external load. A bar graph of liquid retention is shown in FIG. 24. The bar graph shows that the higher absorption capacity of the commercial nonwoven is offset by a relatively low liquid retention. Meanwhile, the NFC coated samples have better liquid retention overall, with the anionic NFC coating showing the highest value of 28.3%. The NFC coating, NFC coating type 4 and HW-NFC coating all have very similar liquid retention.

[0271] NFC amount (g / m 2 A simple graph of the correlation between the x-axis position of the data points for the LW-NFC coating is shown in Figure 25. Furthermore, the x-axis position of the data points for the LW-NFC coating is expected to be distorted and is in fact 7 g / m 2 It is located near the sign.

[0272] Table 5 shows the results from another test lot of a non-sterile medical product according to an embodiment. The table also provides a standard method suitable for determining the properties measured.

[0273] [Table 5]

[0274] The embodiments of the present disclosure also include the following aspects. <1> Providing an aqueous dispersion of nanofibril cellulose, - providing a nonwoven fabric; - immersing the nonwoven fabric in an aqueous dispersion of the nanofibril cellulose to form a coating on the nonwoven fabric; - passing the soaked nonwoven fabric through a preset gap to define a thickness of the coating on the soaked nonwoven fabric without pressing; and - dewatering the soaked nonwoven fabric; - optionally repeating said dipping and passing through said gap at least once, 2. A method of making a medical product, preferably a continuous process, comprising obtaining the medical product by <2> and passing the soaked nonwoven fabric through a preset gap formed between two regulating parts, such as between a pair of rollers, between a roller and a blade, between a roller and a plate, between a pair of plates, or between a pair of blades, to define a thickness of the soaked nonwoven fabric. <1> The method described above. <3> The dewatering is carried out by evaporation (e.g., by non-contact drying (e.g., non-contact drying using an infrared dryer, a floating dryer, or an impingement dryer) or by contact drying (e.g., contact drying using a press dryer, a cylinder dryer (drying cylinder), or a belt dryer)); <1> or <2> The method described above. <4> The nanofibril cellulose, when dispersed in water, has a zero shear viscosity in the range of 1000 to 100000 Pa s (e.g., in the range of 5000 to 50000 Pa s) and a yield stress in the range of 1 to 50 Pa (e.g., in the range of 3 to 15 Pa) when measured by a rotational rheometer at a concentration of 0.5% by weight (w / w) in an aqueous medium at 22°C ± 1°C, and / or the nanofibril cellulose has an average fibril diameter of 200 nm or less (e.g., in the range of 1 to 200 nm). <1> ~ <3> 2. The method according to claim 1 , <5> 1. A medical product comprising a support layer and an absorbent layer as a coating on the support layer, the support layer comprising a nonwoven fabric, and the absorbent layer comprising unpressed nanofibril cellulose having an average fibril diameter of 200 nm or less (e.g., <1> ~ <4> (a) the medical product obtained by any one of the methods described above. <6> The nonwoven fabric is impregnated with nanofibril cellulose, preferably with a content of nanofibril cellulose decreasing from the surface of the nonwoven fabric towards the center of the nonwoven fabric between the surfaces. <5> 2. The medical product described in <7> The nonwoven fabric comprises a natural fabric (e.g. gauze (e.g. cellulose or cotton fabric)), a synthetic or semi-synthetic fabric (e.g. viscose or polyester), or a mixture thereof (preferably a mixture of polypropylene and cellulose, or a mixture of polypropylene, polyester and cellulose); <5> or <6> 2. The medical product described in <8> The nanofibril cellulose comprises chemically unmodified nanofibril cellulose, preferably also enzymatically unmodified nanofibril cellulose; <5> ~ <7> 2. A medical product according to any one of claims 1 to 11. <9> The nanofibril cellulose includes chemically anion-modified nanofibril cellulose having an average fibril diameter of 50 nm or less (for example, within a range of 1 to 50 nm). <5> ~ <8> 2. A medical product according to any one of claims 1 to 11. <10> Liquid retention is within the range of 14.5-40% (e.g., 20-30% (e.g., 22-30%)); <5> ~ <9> 2. A medical product according to any one of claims 1 to 11. <11> Moisture vapor transmission rate (MVTR) measured by SFS-EN-13726-2 is 4000-5500g / m 2* Within a 24-hour period (e.g., 4000-5000 g / m 2* 24 hours or 4400-5000g / m 2* within a 24 hour period) <5> ~ <10> 2. A medical product according to any one of claims 1 to 11. <12> one or more bioactive agents and / or one or more therapeutic agents; <5> ~ <11> 2. A medical product according to any one of claims 1 to 11. <13> The moisture content is within the range of 0 to 10% (e.g., within the range of 1 to 10%). <5> ~ <12> 2. A medical product according to any one of claims 1 to 11. <14> For use in treating and / or covering skin wounds or other injuries (e.g., deep skin wounds, including dermal injuries); <5> ~ <13> 2. A medical product according to any one of claims 1 to 11. <15> For use in treating skin wounds (preferably by a method comprising applying the product onto a wound to absorb a bioactive agent from the wound, storing the bioactive agent in the medical product for a period of time, and allowing the bioactive agent to diffuse back into the wound in a later phase of the wound healing process), <5> ~ <13> 2. A medical product according to any one of claims 1 to 11. <16> For use in administering a bioactive and / or therapeutic agent, <5> ~ <13> 2. A medical product according to any one of claims 1 to 11.

Claims

1. - providing an aqueous dispersion of nanofibril cellulose, - providing a nonwoven fabric, - immersing the nonwoven fabric in an aqueous dispersion of the nanofibril cellulose to form a coating on the nonwoven fabric; - passing the soaked nonwoven fabric through a preset gap, thereby removing excess nanofibril cellulose hydrogel accumulated on the nonwoven fabric and defining the thickness of the coating on the soaked nonwoven fabric without pressing; - dewatering the soaked nonwoven fabric; obtaining medical products by The medical product comprises a support layer comprising the nonwoven fabric and an absorbent layer as a coating on the nonwoven fabric, the absorbent layer comprising unpressed nanofibrillated cellulose; Dewatering the soaked nonwoven fabric does not include vacuuming or pressing. A method for making a medical product.

2. The method of claim 1 , comprising repeating said dipping and passing through said gap at least one time.

3. 3. The method according to claim 1 or claim 2, further comprising passing the soaked nonwoven fabric through a preset gap formed between two regulating parts to define a thickness of the soaked nonwoven fabric.

4. The method according to claim 3 , wherein the regulating part comprises a pair of rollers, a roller and a blade, a roller and a plate, a pair of plates, or a pair of blades.

5. The method according to any one of claims 1 to 4, wherein the dehydration is carried out by evaporation, by using non-contact drying, or by using contact drying.

6. 6. The method of claim 5, wherein the non-contact drying comprises drying with an infrared dryer, a floating dryer, or an impingement dryer, and the contact drying comprises drying with a press dryer, a cylinder dryer (drying cylinder), or a belt dryer.

7. 7. The method according to claim 1, wherein the nanofibril cellulose, when dispersed in water, gives a zero shear viscosity in the range of 1000 to 100000 Pa s and a yield stress in the range of 1 to 50 Pa when measured by rotational rheometer at a concentration of 0.5% by weight (w / w) in aqueous medium at 22°C±1°C, and / or the nanofibril cellulose has an average fibril diameter of 200 nm or less.

8. 8. The method according to claim 1, wherein the nanofibril cellulose, when dispersed in water, gives a zero shear viscosity in the range of 5,000 to 50,000 Pa s and a yield stress in the range of 3 to 15 Pa when measured by rotational rheometer at a concentration of 0.5% by weight (w / w) in aqueous medium at 22°C±1°C, and / or the nanofibril cellulose has an average fibril diameter in the range of 1 to 200 nm.

9. 1. A medical product comprising a support layer and an absorbent layer as a coating on said support layer, said support layer comprising a nonwoven fabric, said absorbent layer comprising unpressed nanofibrillar cellulose having an average fibril diameter of 200 nm or less.

10. 10. The medical product of claim 9, wherein the nonwoven fabric is impregnated with nanofibrillar cellulose.

11. 11. The medical product of claim 10, wherein the nanofibrillar cellulose content decreases from the surface of the nonwoven fabric towards the center of the nonwoven fabric between the surfaces.

12. The medical product according to any one of claims 9 to 11, wherein the nonwoven fabric comprises a natural fabric, a synthetic fabric or a semi-synthetic fabric, or a mixture thereof.

13. 13. The medical product of claim 12, wherein the natural fabric comprises cellulose or cotton fabric, the synthetic or semi-synthetic fabric comprises viscose or polyester, or the nonwoven fabric comprises a blend of polypropylene and cellulose, or a blend of polypropylene, polyester and cellulose.

14. The medical product of any one of claims 9 to 13, wherein the nanofibril cellulose comprises nanofibril cellulose that has not been chemically modified.

15. The medical product of any one of claims 9 to 13, wherein the nanofibril cellulose comprises chemically anionically modified nanofibril cellulose having an average fibril diameter of 50 nm or less.

16. A medical product according to any one of claims 9 to 15, having a liquid retention in the range of 14.5 to 40%.

17. Moisture vapor transmission rate (MVTR) measured by SFS-EN-13726-2 is 4000-5500 g / m ２＊ The medical product according to any one of claims 9 to 16, wherein the duration of the effect is within 24 hours.

18. 18. The medical product of any one of claims 9 to 17, comprising one or more cosmetic agents, one or more bioactive agents, and / or one or more therapeutic agents.

19. A medical product according to any one of claims 9 to 18, having a moisture content in the range of 0 to 10%.

20. A medical product according to any one of claims 9 to 19 for use for treating and / or covering skin wounds or other injuries.

21. 21. The medical product of claim 20 for use to treat and / or cover deep skin wounds, including dermal injuries.

22. 20. A medical product according to any one of claims 9 to 19 for use to treat a skin wound by a method comprising applying the product onto a wound to absorb a bioactive agent from the wound, storing the bioactive agent in the medical product for a period of time, and allowing the bioactive agent to diffuse back into the wound in a later phase of the wound healing process.

23. A medical product according to any one of claims 9 to 19 for use for administering a bioactive and / or therapeutic agent.

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