Method for producing cutting seedlings

Abstract

To provide a method of producing a rooted cutting allowing efficient rooting of a soft scion.SOLUTION: The method of producing a rooted cutting comprises a rooting and culturing step of covering a scion of a broad-leaved or deciduous needle-leaved tree plant with a translucent protective material, inserting the scion in medium, placing the medium such that at least a portion of the medium is in contact with a water-absorbing member, and performing bottom watering for rooting from the scion.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a method for producing cuttings seedlings.

Background Art

[0002] Cutting is a method of rooting artificially cut plant tissues in a rooting bed to create an independent plant, and it is an excellent method for mass-propagating genetically uniform seedlings. As conditions for rooting culture of cuttings of broad-leaved trees and some conifers, a humidity of 80% or more is required. As a method of increasing humidity, there are methods such as sealed cuttings and repeated mist spraying into the cuttings. However, in general sealed cuttings, watering is done by sub-irrigation, so the medium becomes in a state of excessive moisture and poor air permeability easily occurs. Also, in the method of repeating mist spraying, since overhead watering to the above-ground part is frequently performed, there is a problem that the cuttings are easily damaged.

[0003] Patent Document 1 describes that by installing a medium in which cuttings are inserted so that at least a part of the medium is in contact with a water-absorbing member, watering is performed through the water-absorbing member, and rooting is carried out from the cuttings, the moisture environment can be made uniform and the rooting rate can be improved.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] Although the method of Patent Document 1 has good air permeability, it is difficult to maintain humidity, and it may be difficult to root so-called soft cuttings with an underdeveloped cuticle layer. An object of the present invention is to provide a method for producing cuttings seedlings capable of efficiently rooting soft cuttings.

Means for Solving the Problem

[0006] The present invention provides the following [1] to

[10] . 〔1〕A method for producing cuttings seedlings, comprising a rooting culture step of covering cuttings of broad-leaved trees or deciduous coniferous trees with a light-transmissive protective material, placing the medium in which the cuttings are inserted so that at least a part of the medium is in contact with a water-absorbing member, and performing bottom irrigation to cause rooting from the cuttings. 〔2〕The method according to [1], wherein the light-transmissive protective material is in the form of a film or a container. 〔3〕The method according to [1] or [2], wherein the humidity inside the light-transmissive protective material is 80% or more. 〔4〕The method according to [1] to [3], wherein the moisture content of the medium is 60% or less. 〔5〕The method according to any one of [1] to [4], wherein the water-absorbing member is in the form of a sheet or a mat. 〔6〕The method according to any one of [1] to [5], wherein the broad-leaved tree plant is a Prunus plant or a Eucalyptus plant. 〔7〕The method according to any one of [1] to [6], wherein the deciduous coniferous tree plant is a Larix plant. 〔8〕The method according to any one of [1] to [7], wherein the rooting culture step is a step of storing the medium in a culture container having an opening at least at substantially the bottom, placing the culture container on the water-absorbing member so that the medium is in contact with the water-absorbing member through the opening, and performing irrigation. 〔9〕The method according to any one of [1] to [8], wherein the rooting culture step is performed for 2 weeks to 10 months. 〔10〕A light-transmissive protective material, A medium for inserting cuttings, A culture container for storing the medium in which the cuttings are inserted, and A water-absorbing member, A kit for raising cuttings seedlings of plants for carrying out the method according to any one of [1] to [9].

Advantages of the Invention

[0007] According to the method of the present invention, not only can the air permeability in the medium be maintained, but it is also possible to keep the cuttings at high humidity using a light-transmitting covering material, thereby improving the rooting rate from soft cuttings such as broad-leaved or deciduous coniferous plants.

Embodiments for Carrying Out the Invention

[0008] 〔1. Method for Producing Cutting Seedlings〕 〔Rooting Culture Step〕 (Cutting) In the present invention, cuttings of broad-leaved or deciduous coniferous plants from which cutting seedlings are desired to be obtained are used. The broad-leaved plants may be either deciduous broad-leaved trees or evergreen broad-leaved trees. Deciduous broad-leaved trees account for the majority of broad-leaved plants. For example, plants of the genus Prunus (e.g., Prunus spp., Prunus mume, Prunus tomentosa), plants of the genus Quercus (e.g., Quercus acutissima), plants of the genus Vitis, plants of the genus Malus, plants of the genus Rosa, plants of the genus Jacaranda (e.g., Jacaranda mimosifolia), plants of the genus Pyrus (e.g., Pyrus serotina Rehder, Pyrus pyrifolia), etc. can be mentioned.

[0009] Evergreen broad-leaved trees include, for example, plants of the genus Eucalyptus, plants of the genus Mangifera (e.g., Mangifera indica), plants of the genus Acacia, plants of the genus Myrica, plants of the genus Persea (e.g., Persea americana), plants of the genus Santalum (e.g., Santalum album). Among these, species in which the buds are not protected by bud scales and the cuticle layer of the leaves is underdeveloped (e.g., subtropical / tropical species, sclerophyllous trees, etc., species other than evergreen broad-leaved trees) are preferred, and plants of the genus Eucalyptus are preferred.

[0010] Deciduous conifers are deciduous coniferous trees, and examples thereof include plants of the genus Larix (e.g., Larix kaempferi, Larix gmelinii, etc.).

[0011] The cutting can be at least a part of a plant, and examples include branches such as green branches (current-year branches), mature branches (branches that grew before the previous year), etc.; buds such as terminal buds, axillary buds; leaves, cotyledons; hypocotyls, etc. It is preferably including branches and buds, more preferably green branches (current-year branches) and terminal bud branches. The cutting may include a shoot. Thereby, adventitious root formation can be facilitated. A shoot refers to a tissue having rooting ability, and examples include branches, stems, sprouts, terminal buds, axillary buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos, shoot primordia, and multi-bud bodies derived from specific examples thereof (Japanese Patent Laid-Open No. 8-228621), and sprouts are preferred.

[0012] (Light-transmitting protective material) In the present invention, the cutting is covered with a light-transmitting protective material. Thereby, the humidity of soft cuttings such as deciduous tree plants and current-year branches can be maintained within an appropriate range, and rooting can be efficiently performed.

[0013] The light-transmitting protective material only needs to have light transmissivity (preferably, the light transmittance is 80% or more, more preferably 85% or more, still more preferably 90% or more) and substantially not allow moisture to pass through. Examples of the material include resins such as polyethylene, polyvinyl chloride, polyethylene terephthalate, and fibers such as glass and non-woven fabric. Examples of the shape of the light-transmitting protective material include film shape, container shape, and sheet shape. As the film-shaped or sheet-shaped light-transmitting protective material, agricultural polyethylene film and agricultural vinyl can be used. As the container-shaped light-transmitting protective material, seedling caps (e.g., made of resin or non-woven fabric) can be used.

[0014] By covering with a light-transmissive protective material, the moisture content of the culture medium can be kept low while increasing the humidity. The humidity can be adjusted such that the average value during the rooting culture period is preferably 80% or more, more preferably 85% or more. Thereby, rooting from soft cuttings such as current-year branches of broad-leaved trees and deciduous coniferous trees can be promoted, and seedling raising can be efficiently performed. There is no particular limitation on the upper limit. The humidity may be measured in real time using a field management system and its average value may be calculated. The same method was used for measurement in the subsequent examples as well.

[0015] In this specification, the moisture content means the volumetric water content (%) of the rooting culture medium. The moisture content of the culture medium is preferably 60% or less, more preferably 45% or less, and particularly preferably 30% or less. The moisture content is measured by a measurement method using a soil moisture sensor (for example, WD-3, manufactured by A·R·P Co., Ltd.) in real time using a field management system, and its average value may be calculated. The same method was used for measurement in the subsequent examples as well.

[0016] (Water-absorbing member) In the present invention, the cutting bed is placed on a water-absorbing member. Thereby, an appropriate amount of water can be supplied to the cuttings by bottom irrigation, and air permeability can be maintained. The water-absorbing member may be any member having water absorption, and examples thereof include processed products such as sponges, non-woven fabrics, woven fabrics, and papers made of a water-absorbing material itself or a material containing a water-absorbing component (for example, a polyester non-woven fabric), and non-woven fabrics are preferred. The water-absorbing member may contain any optional component other than water absorption such as an antibacterial agent as needed. The shape of the water-absorbing member is not particularly limited, and examples thereof include a mat shape and a sheet shape. Examples of the bottom water supply mat include those described in JP-A-2013-100269.

[0017] (Positions of the culture medium, light-transmissive protective material, and water-absorbing member) The cutting is coated with a light-transmissive protective material, and the medium into which the cutting is inserted is installed such that at least a part of the medium is in contact with a water-absorbing member. Thereby, the humidity around the cutting can be maintained at a high level, the moisture environment of the cutting can be kept appropriate and constant, a state of excessive humidity can be suppressed, the occurrence of diseases can be suppressed, and a good rooting rate can be obtained. Further, when rooting multiple cuttings simultaneously, not only a good rooting rate can be obtained, but also the growth degree such as the rooting time can be made uniform, so that multiple cuttings can be collectively transferred to the seedling raising process. The light-transmissive protective material usually covers the medium into which the cutting is inserted. As a covering method, for example, there is a method in which the medium into which the cutting is inserted is placed on a water-absorbing member, the light-transmissive protective material is placed over the upper part of the cutting, and the ends of the light-transmissive protective material and the water-absorbing member are fixed so as to be in contact with each other. It is preferable that the light-transmissive protective material and the water-absorbing member are detachably fixed. Thereby, it can be adjusted to an appropriate humidity at the time of rooting. Fixing means such as pins and picks may be used for fixing. The part of the medium in contact with the water-absorbing member may be one or two or more, preferably includes at least a part of the substantially bottom of the medium, and is preferably at least a part of the substantially bottom of the medium.

[0018] (Waterproof member) In the rooting culture process, a waterproof member may be used. The waterproof member can be installed below the water-absorbing member. Thereby, leakage of water from the water-absorbing member can be suppressed, and irrigation of the cutting can be efficiently performed. The waterproof member may be any member having waterproof properties, and its shape is not particularly limited. Examples thereof include a mat shape and a sheet shape. Examples of the waterproof member include a vinyl sheet and a film.

[0019] (Light-shielding member) In the rooting culture process, a light-shielding member may be used. The light-shielding member can be installed by overlapping it with the light-transmissive member (for example, on the outer layer side of the light-transmissive member). Thereby, light shielding can be performed and rooting can be promoted. The light shielding rate is preferably 30% or more and 70% or less, and more preferably 40% or more and 60% or less.

[0020] (Culture medium) The culture medium is not particularly limited as long as it can support the cutting and has water absorption and air permeability. The culture medium is not particularly limited as long as it can hold the cutting in a supported state during the rooting culture process, and conventional culture media can be used. Examples of the culture medium include natural soils such as sand and soil (e.g., red volcanic soil); artificial soils such as rice husk charcoal, coconut fiber, vermiculite, perlite, peat moss, and glass beads; porous molded products such as foamed phenolic resin and rock wool; solidifying agents (e.g., agar or gellan gum), and the like.

[0021] The culture medium may contain fertilizer components. Examples of the fertilizer components include components that can serve as a source of plant nutrients such as inorganic components, silver ions, antioxidants, carbon sources, vitamins, amino acids, and plant hormones. The form of the fertilizer components is not particularly limited and may be either a solid (e.g., powder, granule) or a liquid (e.g., liquid fertilizer).

[0022] Examples of the inorganic components include elements such as nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, iron, manganese, zinc, boron, molybdenum, chlorine, iodine, cobalt, etc., and inorganic salts containing these. Examples of the inorganic salts include potassium nitrate, ammonium nitrate, ammonium chloride, sodium nitrate, potassium hydrogen phosphate, disodium hydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, zinc sulfate, copper sulfate, sodium sulfate, calcium chloride, magnesium chloride, boric acid, molybdenum trioxide, sodium molybdate, potassium iodide, cobalt chloride, etc., and hydrates thereof. As the inorganic component, one kind can be selected from the above specific examples, or two or more kinds can be used in combination. In the medium used in the present invention, it is preferable that nitrogen, phosphorus, and potassium are contained as essential elements. Therefore, among the specific examples of these inorganic components, nitrogen, phosphorus, potassium, inorganic salts containing nitrogen, inorganic salts containing phosphorus, and inorganic salts containing potassium are preferable, and nitrogen, phosphorus, potassium, and inorganic salts containing nitrogen are more preferable. When the inorganic component is one kind, it is preferably added so as to be about 1 μM to about 100 mM, and more preferably added so as to be about 0.1 μM to about 100 mM. When two or more kinds are combined, it is preferably added so as to be about 0.1 μM to about 100 mM for each, and more preferably added so as to be about 1 μM to about 100 mM.

[0023] Examples of silver ions include silver compounds (silver ion sources) such as silver thiosulfate (STS, AgS 4 O 6 ), silver nitrate, etc., and STS is preferable. STS takes the form of thiosulfate silver ions in the medium and is presumed to be negatively charged, which can contribute to promoting the rooting and elongation of healthy roots. The concentration of silver ions added to the medium depends on the type of silver ion source and other culture conditions, etc. As the concentration of the silver ion source, it is preferably about 0.5 μM or more and about 6 μM or less, and more preferably about 2 μM or more and about 6 μM or less.

[0024] Examples of antioxidants include ascorbic acid and sulfites, with ascorbic acid being preferred. Since ascorbic acid has low residual properties in the medium, environmental pollution can be suppressed. The concentration of the antioxidant added to the medium is preferably about 5 mg / l or more and about 200 mg / l or less, and more preferably about 20 mg / l or more and about 100 mg / l or less.

[0025] As the carbon source, carbohydrates such as sucrose and their derivatives; organic acids such as fatty acids; and compounds such as primary alcohols such as ethanol can be used. As the carbon source, one type can be selected from the above specific examples, or two or more types can be used in combination. The carbon source is preferably added to the medium to be about 1 g / l to about 100 g / l, and more preferably to be about 10 g / l to about 100 g / l. However, when cultivation is carried out while supplying carbon dioxide gas, the medium does not need to contain a carbon source and preferably does not contain it. Organic compounds that can be a carbon source such as sucrose also serve as a carbon source for microorganisms. Therefore, when using a medium added with these, it is necessary to carry out cultivation in a sterile environment. However, by using a medium that does not contain a carbon source, cultivation in a non-sterile environment becomes possible.

[0026] Examples of vitamins include biotin, thiamine (vitamin B1), pyridoxine (vitamin B6), pyridoxal, pyridoxamine, calcium pantothenate, inositol, nicotinic acid, nicotinamide, and / or riboflavin (vitamin B2), etc. As vitamins, one type can be selected from the above specific examples, or two or more types can be used in combination. When using one type of vitamin, it is preferably added to the medium to be about 0.01 mg / l to about 200 mg / l, and more preferably to be about 0.02 mg / l to about 100 mg / l. When using a combination of two or more types, each is preferably added to the medium to be about 0.01 mg / l to about 150 mg / l, and more preferably to be about 0.02 mg / l to about 100 mg / l.

[0027] As amino acids, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and / or lysine can be used. As amino acids, one kind can be selected from the above specific examples, or two or more kinds can be used in combination. Amino acids are preferably added to the medium so as to be about 0.1 mg / l to about 1000 mg / l. In the case of a combination of two or more kinds, it is preferably added to the medium so as to be about 0.2 mg / l to about 1000 mg / l respectively. Examples of plant hormones include rooting promoters such as auxin and cytokinin. Examples of auxin include naphthalene acetic acid (NAA), indole acetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4-D), indole butyric acid (IBA) and their derivatives, etc. One or more kinds selected from these or a combination of two or more kinds can be used. Examples of cytokinin include benzyl adenine (BA), kinetin, zeatin and their derivatives, etc. One or more kinds selected from these or a combination of two or more kinds can be used. The plant hormone is preferably auxin or a combination of auxin and cytokinin.

[0028] When using one kind of plant hormone in the medium, the concentration is preferably 0.001 mg / l to 10 mg / l, and more preferably 0.01 mg / l to 10 mg / l. When there are two or more kinds of plant hormones, each is preferably 0.001 mg / l to 10 mg / l, and more preferably 0.01 mg / l to 10 mg / l. The method of adding the plant hormone is not particularly limited, and it may be added according to the instructions of commercially available products. For example, methods such as applying the powder to the base of the cutting and adding it to the medium can be mentioned.

[0029] The medium may be used continuously without being exchanged through the method of the present invention, or may be exchanged during the process. From the viewpoint of work simplicity, it is preferable to use it continuously without being exchanged through the method of the present invention. Fertilizer components may be replenished in the medium during the process of the method of the present invention.

[0030] (Culture container) In the rooting culture step, it is preferable to store the medium in which the cuttings are inserted in a culture container. This facilitates adjustment of the portion where the medium and the water-absorbing member are in contact, and the transfer to the seedling raising step after the rooting culture step can be smoothly performed by omitting complicated operations. The culture container preferably has an opening at least at substantially the bottom. Thereby, bottom irrigation through the water-absorbing member into the medium can be efficiently performed. The position of the opening may be at least a part of the substantially bottom (bottom surface and side surface close to the bottom surface). The shape and form of the opening are not particularly limited. For example, it may be two or more scattered holes, or a net existing on the bottom surface by one or two or more. The culture container is preferably a container that can store the medium in which one cutting is inserted, or a unit for inserting the medium in which one cutting is inserted is connected or divided into the unit, and a plurality of cuttings can be inserted as a whole. These containers may be held on a tray as long as the tray does not interfere with the contact point between the medium and the water-absorbing member.

[0031] The culture container is not particularly limited as long as it holds a medium (such as soil) and has an opening at least at substantially the bottom. For example, a cell tray, a container (e.g., the container described in JP-A-2017-079706, a multi-cavity container (JFA-150, JFA-300), etc.), a seedling raising pot, a planter, and a bat (a box-shaped container having a net-like opening on the bottom surface or side surface. It may be one for inserting a plurality of cuttings together) can be mentioned. The material of the culture container is also not particularly limited, and examples include resin, glass, and wood.

[0032] (Insertion) The method of inserting cuttings into the culture medium may be appropriately selected according to the type of culture medium, culture conditions, etc. In addition, it is also preferable to apply physical stimuli such as damaging the base of the cutting when inserting it into the culture medium in order to improve the rooting rate. The base of the cutting means one end of the cutting, which is the region where roots are formed (opposite to the end where leaves are formed). When using a multi-bud body as the cutting, the base is the region having the cutting surface when dividing the multi-bud body. The size (such as the size and shape) of the wound on the base of the cutting is not particularly limited. For example, when using a multi-bud body as the cutting, it is preferable to make a wound such that it forms a cross shape when the base of the cutting (the above-mentioned cutting surface) is viewed from the front direction. When making the wound, instruments such as scissors and knives can be used.

[0033] (Irrigation) In the rooting culture step, the cuttings are irrigated through a water-absorbing member. That is, water is supplied to the water-absorbing member, and the moisture is supplied to the cuttings through the portion where the culture medium and the water-absorbing member are in contact. It is preferable to supply water to the water-absorbing member so that the culture medium becomes moist and / or so that the water-absorbing member is in a state of uniformly absorbing water. Thereby, the moisture environment of the culture medium can be maintained moderately, constantly, and uniformly. The irrigation operation may be performed either manually or by an automatic irrigation device.

[0034] The culture period of the rooting culture step varies depending on the plant species, but is usually 2 weeks to 10 months, preferably 4 weeks to 6 months, and particularly preferably 2 months to 6 months. The rooting culture step may be continued until rooting is observed from the cuttings.

[0035] 〔Seedling raising step〕 After the completion of the rooting culture step, the rooted cuttings can be raised as seedlings to obtain cuttings.

[0036] (Culture medium) The seedling raising process can be carried out according to conventional methods. For example, it is more preferable to directly use the medium in which the cuttings after rooting have been inserted in the seedling raising process. Examples of the medium are the same as those of the medium described in the rooting culture process. In the seedling raising process, it is preferable to install a part of the medium in contact with the air layer. This enables air root pruning and suppresses looping, so that cuttings with improved survival after planting can be provided. The part of the medium in contact with the air layer may be one or two or more, preferably including at least a part of the substantially bottom of the medium, and preferably being at least a part of the substantially bottom of the medium. The part of the medium in contact with the air layer preferably includes at least the same part as a part of the medium that was in contact with the water-absorbing member in the rooting culture process, and more preferably is the same part. The air layer is provided in contact with a part of the medium, and preferably is provided under the substantially bottom of the medium. The method of providing the air layer is not particularly limited. For example, there is a method of installing the medium or the container storing the medium on a tray, stand, rack, etc. and adjusting so that the container is in contact with the air layer.

[0037] (Container) In the seedling raising process, it is preferable to store the medium in a container. The container may be a different container from the culture container used in the rooting culture process or the same container, but the same container is preferable, and it is more preferable to directly use the culture container used in the rooting culture process in the seedling raising process. Thereby, the transition to the seedling raising process after rooting culture can be smoothly carried out by omitting complicated operations. Examples of the container are the same as those of the culture container described in the rooting culture process.

[0038] (Irrigation, humidity) The irrigation method in the seedling raising process is not particularly limited, and overhead irrigation may be used. The humidity conditions are also not particularly limited.

[0039] The culture period of the seedling raising process can be appropriately set according to the plant species, climate conditions of the transplanting / planting location, etc.

[0040] [Other general conditions] In the rooting culture process and the seedling raising process, conditions other than those described above are not particularly limited as long as they allow the cuttings to root and grow seedlings. The conditions may be the same or different in the rooting culture process and the seedling raising process. It is difficult to generally define the conditions according to the type, part, state of the cuttings, type of medium, etc., but an example will be given and explained below.

[0041] (Location for rooting and seedling raising) The location for rooting and seedling raising may be a closed space (e.g., inside a plastic greenhouse, inside a carbon dioxide culture room, inside a greenhouse, indoors) or an open space (e.g., outdoors), but a closed space is preferred. This makes it easier to adjust conditions such as temperature and humidity.

[0042] (Temperature for rooting and seedling raising) The temperature in the environment for rooting and seedling raising is not particularly limited as long as it allows the cuttings to root and grow seedlings. For example, it is preferably 20 to 40 °C.

[0043] (pH of the medium) The pH of the medium is preferably 4 to 8, and more preferably about pH 4 (e.g., pH 4 to 6). This can suppress the growth of various bacteria. The pH of the rooting medium and the seedling raising medium may be different or the same.

[0044] (Light quantity) The light irradiation conditions are not particularly limited, and sunlight or artificial light may be used. The light intensity is not particularly limited, but is expressed as the photosynthetically active photon flux density and is preferably about 10 μmol / m 2 / s or more and about 1000 μmol / m 2 / s or less, and more preferably about 50 μmol / m 2 / s or more and about 500 μmol / m 2 / s or less.

[0045] The light wavelength is not particularly limited, but it is preferably carried out under irradiation with light containing a wavelength component of about 650 nm or more and about 670 nm or less and a wavelength component of about 450 nm or more and about 470 nm or less in a ratio of 9:1 to 7:3, and more preferably under irradiation with light containing these wavelength components in a ratio of 9:1 to 8:2. By irradiating with light containing such wavelength components, rooting from plants can be further promoted.

[0046] (Carbon dioxide concentration) Carbon dioxide in the environment for rooting and seedling raising is preferably supplied so as to be usually 300 ppm or more and 2000 ppm or less, and preferably 800 ppm or more and 1500 ppm or less. Control of the supply amount of carbon dioxide can be carried out using equipment such as an artificial weather instrument or a culture container having a carbon dioxide-permeable membrane at the opening.

[0047] [2. Kit for raising cuttings of plants] The method of the present invention can be preferably implemented by a kit for raising cuttings of plants, which includes a medium for inserting cuttings, a culture container for storing the medium with the cuttings inserted, a light-transmitting protective material, a water-absorbing member, and a tray, stand or pedestal. Examples of the medium, culture container, water-absorbing member, tray, stand, and pedestal are the same as those described in the above method of the present invention. The kit of the present invention may or may not further include cuttings before rooting. The kit of the present invention may further have a waterproof member. Also, as described in the above method of the present invention, it is preferable to use the same ones for both the medium and the culture container in both steps, so it is not necessary to prepare them for each step, but they may be prepared for each step.

Examples

[0048] The present invention will be described below with reference to the following examples, but the present invention is not limited thereto.

[0049] Example 1 On May 11, 2020, apical bud branches 5 - 15 cm long sprouted from the mother larch trees were collected, and all the leaves in the range of 2 - 3 cm from the lower part were cut off to prepare cuttings. As the culture container, a cell tray (72 holes, 40 cc, hole size 36 mm, length 539 mm, width 277 mm, height 45 mm, manufactured by Meiwa Co., Ltd.) was used. Small red clay soil (manufactured by Sashima Shosha Co., Ltd.) and peat moss (manufactured by Toho Co., Ltd.) were mixed in a 1:1 ratio and filled to prepare the cutting bed. To the base (cut part) of the cuttings prepared as described above, 5 - 10 mg of the powder of Rootone (registered trademark) (white powder containing the plant hormone NAA, manufactured by Ishihara Bioscience Co., Ltd., NAA concentration is 0.4%) was applied, and then the cuttings were inserted into the cutting bed up to 1.5 - 2.5 cm from the base. Inside a normal vinyl greenhouse, a bottom watering mat (product name: Unitika Love Mat (registered trademark) U, polyester long fiber non-woven fabric, thickness 2.2 mm) was installed on top of the vinyl sheet, and the cell tray inserted on the bottom watering mat was installed so that the bottom directly touched the bottom watering mat. Irrigation was not carried out directly on the plants and the cell tray, but on the bottom watering mat until the bottom watering mat was sufficiently wet. The soil was moistened by bottom irrigation using the bottom watering mat. The irrigation work was carried out either by manual irrigation or an automatic irrigation device. Then, the cell tray was covered with a seedling cap (product name: Akasaka Seedling Cap No. 6, 70×34.5 cm, height 22.5 cm, made of polyethylene terephthalate, light transmittance 90% or more) and root culture was carried out for 2 months until July. Water was used as the medium. During the root culture period, the moisture content and humidity of the medium were measured in real time by the field management system "Midori Cloud" (Serac Co., Ltd.), and the average values during the root culture period were calculated respectively. The seedling cap was not removed or attached during the culture period. The cuttings after culture were observed with the naked eye, and if roots were confirmed, it was judged that rooting had occurred (Table 1).

[0050] Example 2 The same procedure as in Example 1 was carried out except that the cell tray was covered in a tunnel shape with a polyethylene film (transparent multi, size width 230 cm, length 1 m, thickness 0.03 mm, manufactured by Agridream Co., Ltd., light transmittance 90% or more) (Table 1). During the root culture period, the film was not removed or attached.

[0051] Comparative Example 1 It was carried out in the same manner as in Example 1, except that 3 to 4 cm of water was put in the vat and the cell tray was immersed to perform bottom watering (waist watering) (Table 1).

[0052] Comparative Example 2 It was carried out in the same manner as in Example 1, except that overhead watering by mist spraying was carried out once every 10 minutes for 1 minute from 6:00 to 18:00 during the day (Table 1).

[0053] Comparative Example 3 It was carried out in the same manner as in Example 1, except that it was not covered with a seedling cap (Table 1).

[0054] [Table 1]

[0055] Compared with Comparative Example 1 in which the cuttings were covered with a seedling cap and watering was carried out by waist watering, Comparative Example 2 in which the cuttings were not covered and watering was carried out by mist spraying, and Comparative Example 3 in which watering was carried out by a bottom water absorption mat but the cuttings were not covered, in the Example in which the cuttings were covered and watering was carried out by a bottom water absorption mat, the rooting rate and survival rate of the cuttings were high.

[0056] Example 3 It was carried out in the same manner as in Example 1, except that apical bud branches of 5 to 10 cm sprouted from the Eucalyptus albida mother tree on May 12, 2020 were collected and subjected to rooting culture for 1 month until June (Table 2).

[0057] Example 4 It was carried out in the same manner as in Example 3, except that the cell tray was covered with the same polyethylene film as in Example 2 (Table 2).

[0058] Comparative Example 4 It was carried out in the same manner as in Example 3, except that 3 to 4 cm of water was put in the vat and the cell tray was immersed to perform bottom watering (waist watering) (Table 2).

[0059] Comparative Example 5 Except for overhead irrigation by mist sprinkling once every 10 minutes for 1 minute from 6 to 18 o'clock, it was carried out in the same manner as in Example 3 (Table 2).

[0060] Comparative Example 6 Except for not covering with a seedling cap, it was carried out in the same manner as in Example 3 (Table 2).

[0061]

Table 2

[0062] Compared with Comparative Example 4 in which the cuttings were covered with a seedling cap and irrigation was carried out by sub-irrigation, Comparative Example 5 in which the cuttings were not covered and irrigation was carried out by mist sprinkling, and Comparative Example 6 in which irrigation was carried out by a bottom water absorption mat but the cuttings were not covered, in the Example in which the cuttings were covered and irrigation was carried out by a bottom water absorption mat, the rooting rate and survival rate of the cuttings were high.

[0063] Example 5 On May 12, 2020, current-year branches germinated from a Yoshino cherry mother tree were collected, adjusted to 5 - 10 cm with one node and one leaf, and except for root culture for 1 month until June, it was carried out in the same manner as in Example 1 (Table 3).

[0064] Example 6 Except for covering the cell tray with the same polyethylene film as in Example 2, it was carried out in the same manner as in Example 5 (Table 3).

[0065] Comparative Example 7 Except for filling the vat with 3 - 4 cm of water and immersing the cell tray to carry out bottom irrigation (sub-irrigation), it was carried out in the same manner as in Example 5 (Table 3).

[0066] Comparative Example 8 Except for overhead irrigation by mist sprinkling once every 10 minutes for 1 minute from 6 to 18 o'clock, it was carried out in the same manner as in Example 5 (Table 3).

[0067] Comparative Example 9 Except for not covering with a seedling cap, it was carried out in the same manner as in Example 5 (Table 3).

[0068]

Table 3

[0069] In Comparative Example 7 where the cuttings were covered with a seedling cap and irrigation was performed by waist water, Comparative Example 8 where the cuttings were not covered and irrigation was performed by mist spraying, and Comparative Example 9 where irrigation was performed using a bottom water absorption mat but the cuttings were not covered, the rooting rate and survival rate of the cuttings were high in the Example where the cuttings were covered and irrigation was performed using a bottom water absorption mat.

[0070] These results indicate that according to the present invention, since the cuttings can be kept at high humidity while maintaining the air permeability in the culture medium, the rooting rate from the cuttings can be improved, and efficient production of cuttings seedlings is possible.

Claims

1. A rooting culture step is included in which a cutting of a broad-leaved tree or a deciduous coniferous tree plant is covered with a light-transmitting protective material, and the medium in which the cutting is inserted is placed so that at least a part of the medium is in contact with a water-absorbing member, and bottom watering is performed to cause rooting from the cutting. The humidity inside the light-transmitting protective material is 85% or more. The moisture content of the medium is 30% or less. The light-transmitting protective material is in the form of a film and covers the medium in a tunnel shape. In the rooting culture step, the light-transmitting protective material is not detached or attached. A method for producing cuttings of plants.

2. The method according to claim 1, wherein the light-transmitting protective material is in the form of a film or a container.

3. The method according to claim 1 or 2, wherein in the rooting culture, the cutting is further covered with a light-shielding member.

4. The method according to any one of claims 1 to 3, wherein the water-absorbing member is in the form of a sheet or a mat.

5. The method according to any one of claims 1 to 4, wherein the broad-leaved tree plant is a plant of the genus Prunus or the genus Eucalyptus.

6. The method according to any one of claims 1 to 5, wherein the deciduous coniferous tree plant is a plant of the genus Larix.

7. The rooting culture step is a step of storing the medium in a culture container having an opening at least substantially at the bottom, placing the culture container on the water-absorbing member so that the medium is in contact with the water-absorbing member through the opening, and performing watering. The method according to any one of claims 1 to 6.

8. The method according to any one of claims 1 to 7, wherein the rooting culture step is performed for 2 weeks to 10 months.

9. A film-shaped light-transmitting protective material, A medium for inserting a cutting, A culture container for storing the medium in which the cutting is inserted, And A water-absorbing member, A kit for raising cuttings of plants for implementing the method according to any one of claims 1 to 8.

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