Composition for Controlling Sugar Beet Brown Spot

Abstract

To provide a composition for pest control of beet brown spot disease causing enormous damage in beet cultivation and a beet brown spot disease pest control method using the disease pest control composition.SOLUTION: The present invention relates to a composition for controlling beet brown spot disease containing isotianil, and a method for controlling beet brown spot disease including at least one application of the composition for controlling beet brown spot disease containing isotianil. The composition for controlling brown spot disease of beet in accordance with the present invention may further contain pest control agents for beet brown spot other than isotianil.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The present invention relates to a composition for controlling sugar beet leaf spot disease that causes great damage in sugar beet cultivation and a method for controlling sugar beet leaf spot disease using the composition.

Background Art

[0002] Sugar beet (Beta vulgaris ssp. vulgaris, also known as: sugar beet) is a biennial crop of the Amaranthaceae family and is widely cultivated in cool temperate regions around the world as the second most important sugar crop after sugarcane. Sugar beet leaf spot disease is regarded as the most important disease of sugar beet. When it occurs frequently, the above-ground stems and leaves disappear, and not only the sugar content in the roots but also the root weight decreases, resulting in great damage (Non-Patent Document 1).

[0003] The pathogen of sugar beet leaf spot disease, Cercospora beticola, overwinters in the residues of diseased leaves and stems left in the field, and in early summer, it produces spores and spreads to sugar beet. Studies have been conducted on the infection conditions of sugar beet leaf spot disease (Non-Patent Document 2), and the establishment of its control method has been aimed at. The key to controlling sugar beet leaf spot disease is to prevent primary infection by prophylactic application of a fungicide. Generally, control is carried out by spraying the fungicide on the stems and leaves. As the agents, DMI agents (difenoconazole, tetraconazole, fenbuconazole, tebuconazole), QoI agents (trifloxystrobin, pyraclostrobin, pyribencarb, azoxystrobin, kresoxim-methyl), kasugamycin, copper agents, manzeb agents, tetrachloroisophthalonitrile agents, etc. are used, and further fungicides based on fluoropyrimidinone derivatives are being studied (Patent Documents 1 to 4).

[0004] Cercospora beticola easily acquires fungicide resistance, and the sensitivity to QoI agents, kasugamycin, and DMI agents has been decreasing worldwide, which has hindered the control of sugar beet leaf spot disease.

[0005] Incidentally, it has been shown that isothiazolecarboxylic acid anilide derivatives are effective in controlling rice blast, an important disease of rice (Patent Document 5). In particular, isotianil (3,4-dichloro-2'-cyano-1,2-thiazole-5-carboxanilide (IUPAC name), CAS No 224049-04-1) has been put into practical use under the names of Stout (registered trademark) or Routin (registered trademark). Isotianil is known as a plant disease resistance inducer and has been shown to activate the defense function (systemic acquired resistance) of plants against pathogenic bacteria that the plants themselves possess (Non-Patent Document 3). In addition, combining isotianil with other fungicides and the like to form a fungicidal and insecticidal composition has been studied (Patent Documents 6 to 10).

[0006] It has been suggested that isotianil may show a certain effect in controlling plant diseases in various plants other than controlling rice blast in rice (Non-Patent Documents 3 to 5). However, no specific study has been made on whether isotianil can be used to control sugar beet plant diseases, and in particular, no study has been made on using isotianil against sugar beet leaf spot disease.

Prior Art Documents

Patent Documents

[0007]

Patent Document 1

Patent Document 2

Patent Document 3

Patent Document 4

Patent Document 5

Patent Document 6

Patent Document 7

Patent Document 8

[0008] [Non-Patent Document 1] Motoshi Shimizuki, "Control Technology of Turnip Diseases and Pests - Brown Spot and Cabbage Moth -", Specialty Seeds and Seedlings, No. 12, pp. 40-43, published in November 2011. [Non-Patent Document 2] Hokkaido Pest Control Institute, "Method for Determining the Start Time of Monitoring Sugar Beet Brown Spot", Summary of Achievements, created in January 2004 (http: / / www.hro.or.jp / list / agricultural / result_pdf / result_pdf2004 / 2004411.pdf). [Non-Patent Document 3] Masatoshi Ogawa et al., "Development of a New Rice Blast Control Agent, Isotianil (Stout (registered trademark))", Sumitomo Chemical Technical Journal, 2011-I, pp. 4-17, published on May 31, 2011. [Non-Patent Document 4] Haruhiko Sakuma et al., "Research on a New Fungicide, Isotianil (Routine (registered trademark)) - First Report: Action Characteristics against Rice Blast and Others", Journal of the Japanese Phytopathological Society, 74(3), p. 267(375), August 2008. [Non-Patent Document 5] Masatoshi Ogawa et al., "Research on a New Fungicide, Isotianil (Routine (registered trademark)) - Second Report: Action Characteristics against Rice Blast and Others", Journal of the Japanese Phytopathological Society, 74(3), p. 267(376), August 2008. [Summary of the Invention] [Problems to be Solved by the Invention]

[0009] The inventors of the present invention have come to the recognition that, while the control of sugar beet leaf spot disease is becoming difficult due to a decrease in sensitivity to fungicides, it is an urgent task to establish a new control method for sugar beet leaf spot disease. Therefore, the problem of the present invention is to provide such a new control method. [Means for Solving the Problems]

[0010] In the course of intensive research to solve the above problems, the inventors of the present invention unexpectedly found that isothianil, known as a fungicide for rice blast disease, exhibits a high control effect against sugar beet leaf spot disease. To utilize such a control effect, a new composition for controlling sugar beet leaf spot disease and a control method using the composition were developed, leading to the completion of the present invention.

[0011] That is, the present invention relates to the following. [1] A composition for controlling sugar beet leaf spot disease containing isothianil. [2] The composition according to [1] above, further containing one or more fungicides and / or insecticides other than isothianil. [3] The fungicide is one or more selected from the group consisting of phenylamide fungicides, aromatic heterocyclic fungicides, methyl benzimidazole carbamate (MBC) fungicides, N-phenylcarbamate fungicides, thiazole carboxamide fungicides, phenylurea fungicides, benzamide fungicides, pyrimidineamine fungicides, pyrazole-MET1 fungicides, succinate dehydrogenase inhibitors (SDHI), QoI fungicides, QiI fungicides, QoSI fungicides, oxidative phosphorylation uncouplers, anilinopyrimidine fungicides, hexopyranosyl antibiotics, glucopyranosyl antibiotics, tetracycline antibiotics, phenylpyrrole fungicides, dicarboximide fungicides, phosphorothiolate fungicides, dithiolane fungicides, AH fungicides, carbamate fungicides, microorganisms (Bacillus sp., Talaromyces sp.) pesticides, oxysterol binding protein inhibitors (OSBPI), demethylation inhibitors (DMI fungicides) (SBI: Class I), SBI (Class III) fungicides, CAA fungicides, MBI-R fungicides, MBI-D fungicides, MBI-P fungicides, benzisothiazole fungicides, thiadiazole carboxamide fungicides, cyanoacetamide = oxime fungicides, phosphonate fungicides, benzenesulfonic acid fungicides, phenylacetamide fungicides, allylphenylketone fungicides, thiazolidine fungicides, pyrimidinone hydrazone fungicides, 4-quinolylacetic acid fungicides, tetrazolyloxime fungicides, copper agents, dithiocarbamate fungicides, phthalimide fungicides, chloronitrile fungicides and bisguanidine fungicides, the composition according to [2] above.

[0012] [4] The insecticide is one or more selected from the group consisting of acetylcholinesterase (AChE) inhibitors, GABA-activated chloride ion channel blockers, sodium channel modulators, nicotinic acetylcholine receptor (nAChR) competitive modulators, nicotinic acetylcholine receptor (nAChR) allosteric modulators, glutamate-activated chloride ion channel (GluCl) allosteric modulators, juvenile hormone analogs, chordotonal organ TRPV channel modulators, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers that disrupt the proton gradient, nicotinic acetylcholine receptor (nAChR) channel blockers, chitin biosynthesis inhibitors, octopamine receptor agonists, mitochondrial electron transport system complex III inhibitors, mitochondrial electron transport system complex I inhibitors (METI), voltage-dependent sodium channel blockers, acetyl-CoA carboxylase inhibitors, mitochondrial electron transport system complex II inhibitors, ryanodine receptor modulators, and chordotonal organ modulators, the composition according to [2] or [3] above. [5] A method for controlling sugar beet leaf spot, comprising applying a composition for controlling sugar beet leaf spot containing isothianil to sugar beet. [6] The method according to [5] above, wherein the application is an application to at least one of the sugar beet plant body, the culture soil of the plant body, and the seeds configured to produce the plant body. [7] The method according to [5] or [6] above, wherein the application is performed two or more times. [8] The method according to any one of [5] to [7] above, wherein the composition for controlling sugar beet leaf spot containing isothianil is the composition according to any one of [1] to [4] above. [9] A method for controlling sugar beet leaf spot, characterized in that the seedbed is treated with isothianil before sowing and / or at the time of sowing.

[10] A method for controlling sugar beet leaf spot, characterized in that the seeds are treated with isothianil and the plant body is treated with isothianil at intervals of one month or more.

[11] A method for controlling sugar beet leaf spot, characterized by treating the soil with isothianil before sowing and / or at the time of sowing, and treating the plant body with isothianil at intervals of one month or more.

[12] Use of isothianil for the treatment and / or prevention of sugar beet leaf spot.

Effect of the Invention

[0013] The composition for controlling sugar beet leaf spot of the present invention acts on sugar beet and can induce resistance to sugar beet leaf spot. Conventional fungicides may generate drug-resistant bacteria, but the control composition of the present invention can control sugar beet leaf spot bacteria without the risk of generating drug-resistant bacteria. In addition, when used in combination with isothianil and a conventional fungicide for sugar beet leaf spot, the effects of each other do not antagonize or inhibit each other, and a better control effect is shown compared with the case of using only isothianil.

[0014] Furthermore, the composition for controlling sugar beet leaf spot of the present invention can obtain a control effect for a longer period than conventional conventional control. Therefore, since the spraying interval can be lengthened, the number of applications and types of chemicals can be reduced, and labor can be saved. When applying conventional conventional control with a large number of applications, farming operations compete with other crop species, and the intended number of applications cannot be achieved, resulting in insufficient control and possibly promoting the generation of drug-resistant bacteria. However, the method of applying the control composition of the present invention using isothianil is expected to achieve a long duration, reduce the number of applications of chemicals, and eliminate competition in farming operations.

[0015] In addition, when applying a method of smearing a chemical on sugar beet seeds (seed smearing) or a method of perfusion of a chemical to a seedbed before transplanting sugar beet plants to a field (seedbed perfusion), etc., labor can be further saved.

Brief Description of the Drawings

[0016]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Modes for Carrying Out the Invention

[0017] The composition for controlling brown spot disease in sugar beet of the present invention contains isothianil. The composition for controlling brown spot disease in sugar beet of the present invention includes not only compositions in a state of being applied to the target plants or seeds using an appropriate device such as a spraying device or a dusting device, but also commercial concentrated compositions that need to be diluted before application to crops.

[0018] The composition for controlling sugar beet brown spot of the present invention may be used as it is, but it is preferably applied in the form of a composition mixed with a carrier containing a solid or liquid diluent. Specifically, the composition for controlling sugar beet brown spot of the present invention can be used in various forms. For example, it may be in the form of dust (D), granule (GR), powder-granule, powder, wettable powder (WP), soluble powder (SP), emulsion (EC), solution (SL), oil (OL), aerosol, microcapsule agent (MC), paste agent, smoke agent, vaporizing agent, coating agent, DL powder, FD agent, fine agent F, fine granule agent F, granule wettable powder (WG, WDG), dry flowable (DF), flowable formulation (SC), sol, suspoemulsion formulation (SE), thick emulsion formulation (EW, CE), microemulsion formulation (ME), seed dressing agent, suspension, tablet, oil, foaming agent, ULV [cold mist, warm mist], etc. As the form of the composition for controlling sugar beet brown spot of the present invention, a solution, dust, seed dressing agent, granule, wettable powder, emulsion or macro capsule, etc. are preferable.

[0019] Here, the carrier means a synthetic or natural inorganic or organic substance that is formulated to assist the active ingredient in reaching the site to be treated and to facilitate the storage, transportation, and handling of the active ingredient compound. Suitable solid carriers include clays such as montmorillonite, kaolinite, and bentonite, diatomaceous earth, clay, talc, vermiculite, gypsum, calcium carbonate, silica gel, ammonium sulfate and other inorganic substances, soybean flour, sawdust, wheat flour and other vegetable organic substances, and urea and the like.

[0020] Suitable liquid carriers include aromatic hydrocarbons such as toluene, xylene, and cumene, paraffinic hydrocarbons such as kerosene and mineral oil, halogenated hydrocarbons such as carbon tetrachloride, chloroform, and dichloroethane, ketones such as acetone and methyl ethyl ketone, ethers such as dioxane, tetrahydrofuran, and diethylene glycol dimethyl ether, alcohols such as methanol, ethanol, propanol, and ethylene glycol, esters such as diethyl phthalate and n-butyl acetate, dimethylformamide, dimethyl sulfoxide, vegetable oil, and water and the like.

[0021] In addition, in order to enhance the efficacy of the composition for controlling sugar beet brown spot disease of the present invention, the following adjuvants can be used alone or in combination according to the purpose, considering the dosage form of the formulation, the application scenario, etc.

[0022] As adjuvants, for the purposes of emulsification, dispersion, spreading, wetting, binding, and stabilization, etc., anionic surfactants such as lignin sulfonates, alkylbenzene sulfonates, alkyl sulfate esters, polyoxyalkylene alkyl sulfates, and polyoxyalkylene alkyl phosphate esters; nonionic surfactants such as polyoxyalkylene alkyl ethers, polyoxyalkylene alkyl aryl ethers, polyoxyalkylene alkyl amines, polyoxyalkylene alkyl amides, polyoxyalkylene alkyl thioethers, polyoxyalkylene fatty acid esters, glycerin fatty acid esters, sorbitan fatty acid esters, polyoxyalkylene sorbitan fatty acid esters, and polyoxypropylene polyoxyethylene block polymers; lubricants such as calcium stearate and waxes; stabilizers such as isopropyl hydrogen diphosphate; and others such as methyl cellulose, carboxymethyl cellulose, casein, and gum arabic can be mentioned, but are not limited to the above.

[0023] The composition for controlling sugar beet brown spot disease of the present invention can be used in combination with pesticides such as fungicides, insecticides, herbicides, and plant growth regulators other than isothianil, soil conditioners, or fertilizer substances, and it is also possible to make a mixed formulation with these. In addition, the composition for controlling sugar beet brown spot disease of the present invention can also be used in combination with the following sugar beet brown spot disease control agents (plant resistance inducers, fungicides, insecticides, etc.) other than isothianil. By combining with these, the efficacy of the composition for controlling sugar beet brown spot disease may be enhanced.

[0024] Specific examples of the bactericides include phenylamide bactericides (e.g., metalaxyl, metalaxyl-M, etc.); aromatic heterocyclic bactericides (e.g., hydroxyisoxazole, etc.); methyl benzimidazole carbamate (MBC) bactericides (e.g., benomyl, thiophanate-methyl, etc.); N-phenylcarbamate bactericides (e.g., diethofencarb, etc.); thiazole carboxamide bactericides (e.g., ethaboxam, etc.); phenylurea bactericides (e.g., pencycuron, etc.); benzamide bactericides (e.g., fluopicolide, etc.); pyrimidineamine bactericides (e.g., diflumetorim, etc.); pyrazole-MET1 bactericides (e.g., tolfenpyrad, etc.); succinate dehydrogenase inhibitors (SDHIs) (e.g., flutolanil, mepronil, isofetamid, fluopyram, thifluzamide, fluxapyroxad, flametopyril, isopyrazam, penflufen, penthiopyrad, boscalid, bixafen, ipfencarbazone, sedaxane, isoflucypram, pyraziflumid, etc.); QoI bactericides (e.g., azoxystrobin, picoxystrobin, mandestrobin, pyraclostrobin, kresoxim-methyl, trifloxystrobin, metominostrobin, orysastrobin, famoxadone, fluoxastrobin, fenamidone, pyribencarb, etc.); QiI bactericides (e.g., cyazofamid, amisulbrom, etc.); QoSI bactericides (e.g., ametoctradin, etc.); oxidative phosphorylation uncouplers (e.g., fluazinam, etc.); anilinopyrimidine bactericides (e.g., cyprodinil, mepanipyrim, etc.); hexopyranosyl antibiotics (e.g., kasugamycin, etc.); glucopyranosyl antibiotics (e.g., streptomycin, etc.); tetracycline antibiotics (e.g., oxytetracycline, etc.); phenylpyrrole bactericides (e.g., fludioxonil, etc.); dicarboximide bactericides (e.g., iprodione, procymidone, etc.); phosphorothiolate bactericides (e.g., iprobenfos, etc.); dithiolane bactericides (e.g., isoprothiolane, etc.); AH bactericides (e.g., tolclofos-methyl, etc.); carbamate bactericides (e.g., propamocarb hydrochloride, etc.); microorganisms (Bacillus sp.)) Pesticides (e.g., Bacillus subtilis, etc.); Oxysterol-binding protein inhibitors (OSBPIs) (e.g., oxathiapiprolin, etc.); Demethylation inhibitors (DMI fungicides) (SBI: Class I) (e.g., triflumizole, prochloraz, cyproconazole, difenoconazole, fenbuconazole, hexaconazole, ipconazole, metconazole, propiconazole,simeconazole, tebuconazole, tetraconazole, prothioconazole, etc.); SBI (Class III) fungicides (e.g., fenhexamid, fenpyrazamine, pyrimethanil, etc.); Polyoxin fungicides (e.g., polyoxin, etc.); CAA fungicides (e.g., dimethomorph, benalaxyl-M-isopropyl, mandipropamid, etc.); MBI-R fungicides (e.g., flutolanil, pyroquilon, tricyclazole, etc.); MBI-D fungicides (e.g., carpropamid, diclocymet, fenoxanil, etc.); MBI-P fungicides (e.g., tolprocarb, etc.); Benzisothiazole fungicides (e.g., probenazole, etc.); Thiadiazole carboxamide fungicides (e.g., tiadinil, etc.); Cyanoacetamide-oxime fungicides (e.g., cymoxanil, etc.); Phosphonate fungicides (e.g., fosetyl, etc.); Benzenesulfonic acid fungicides (e.g., flusulfamide, etc.); Phenylacetamide fungicides (e.g., cyflufenamid, etc.); Allylphenylketone fungicides (e.g., pyriofenone, etc.); Thiazolidine fungicides (e.g., flutianil, etc.); Pyrimidinone hydrazone fungicides (e.g., ferimzone, etc.); 4-Quinolylacetic acid fungicides (e.g., tebufloquin, etc.); Tetrazolyloxime fungicides (e.g., picarbutrazox, etc.); Copper agents, dithiocarbamate fungicides (e.g., manzeb, mancozeb, propineb, thiram, ziram, etc.); Phthalimide fungicides (e.g., captan, etc.); Chloronitrile fungicides (e.g., TPN, etc.); Bisguanidine fungicides (e.g., iminoctadine acetate, iminoctadine albesilate, etc.) and the like can be mentioned.

[0025] Specific examples of the insecticide include acetylcholinesterase (AChE) inhibitors (e.g., alanycarb, benfuracarb, carbaryl, carbosulfan, ethiofencarb, isoprocarb, methomyl, thiodicarb, acephate, chlorpyrifos, fenitrothion, fenthion, fostiazate, imicyafos, malathion, etc.); GABA-activated chloride ion channel blockers (e.g., ethiprole, fipronil, endosulfan, chlordene, etc.); sodium channel modulators (e.g., acrinathrin, cyfluthrin, cyhalothrin, permethrin, deltamethrin, etofenprox, flucythrinate, flumethrin, pyrethrin, silafluofen, tralomethrin, transfluthrin, DDT, etc.); nicotinic acetylcholine receptor (nAChR) competitive modulators (e.g., acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, sulfoxaflor, flupyradifurone, flupyrimin, triflumizole, etc.); nicotinic acetylcholine receptor (nAChR) allosteric modulators (e.g., spinosad, spinetoram, etc.); glutamate-activated chloride ion channel (GluCl) allosteric modulators (e.g., abamectin, emamectin benzoate, lepimectin, milbemectin, etc.); juvenile hormone analogs (e.g., hydroprene, kinoprene, methoprene, phenoxycarb, pyriproxyfen, etc.); chordotonal organ TRPV channel modulators (e.g., pymetrozine, pyrifluquinazon, etc.); mitochondrial ATP synthase inhibitors (e.g., diafenthiuron, fenbutatin oxide, etc.); oxidative phosphorylation uncouplers that disrupt the proton gradient (e.g., chlorfenapyr, etc.); nicotinic acetylcholine receptor (nAChR) channel blockers (e.g., bensultap, cartap, thiocyclam, etc.); chitin biosynthesis inhibitors (e.g., triflumuron, buprofezin, etc.); octopamine receptor agonists (e.g., amitraz, etc.); mitochondrial electron transport system complex III inhibitors (e.g., hydramethylnon, etc.); mitochondrial electron transport system complex I inhibitors (METI) (e.g., pyridaben, tolfenpyrad, etc.);Voltage-dependent sodium channel blockers (e.g., indoxacarb, metaflumizone, etc.); acetyl-CoA carboxylase inhibitors (e.g., spirodiclofen, spirotetramat, etc.); mitochondrial electron transport chain complex II inhibitors (e.g., cyflumetofen, pifubmide, etc.); ryanodine receptor modulators (e.g., chlorantraniliprole, cyantraniliprole, flubendiamide, cyclaniliprole, tetraniliprole, etc.); chordotonal organ modulators (e.g., flonicamid, etc.), and the like.

[0026] When applying the sugar beet leaf spot disease control composition of the present invention, it is applied to at least one of the sugar beet plant bodies (e.g., roots, stems, leaves, etc.), the culture soil of the plant bodies (e.g., the ground adjacent to the plants, the soil configured to support the growth of the plants), and the seeds configured to produce the plant bodies. More specifically, for example, by soil perfusion, soil mixing, drenching at the base of the plant, infallow treatment, drip irrigation, spraying, vaporization, atomization, scattering, dusting, foaming spraying, coating, and as a powder for dry seed treatment, a solution for seed treatment (e.g., seed immersion, seed coating, seed film coating, etc.), a water-soluble powder for seed treatment, a water-soluble powder for slurry treatment, it can be directly implemented, or it can be applied by acting on their surroundings, habitat environment or storage area, etc. When applying to seeds, it can be applied by covering the surface, and further, it can be applied by one or more layers of coating by dry treatment, slurry treatment, liquid treatment, and it can also be used as a raw material for manufacturing pellet seeds, coated seeds, granulated seeds, etc.

[0027] The application of the sugar beet leaf spot disease control composition of the present invention may be carried out once or two or more times. When carried out two or more times, the same application method may be repeated, or it may be combined with different application methods. For example, after applying once to the seeds, it can also be applied twice by spraying on the stems and leaves. In one aspect, the present invention relates to a method for controlling sugar beet leaf spot disease, characterized in that the seedbed is treated with isothianil before sowing and / or at the time of sowing.

[0028] The dosage of isothianil in the application of the composition for controlling sugar beet leaf spot of the present invention is not particularly limited, but in any application method, for example, it is 1 to 1,000 g AI / ha, preferably 10 to 500 g AI / ha, more preferably 12 to 400 g AI / ha. Furthermore, the dosage corresponding to each application method can be selected. As the dosage for seed treatment, it is preferably 1 to 200 g AI / ha, more preferably 10 to 100 g AI / ha. As the dosage for seedbed perfusion, it is preferably 1 to 400 g AI / ha, more preferably 10 to 200 g AI / ha, particularly preferably 40 to 160 g AI / ha. As the dosage for foliage spraying, it is preferably 10 to 500 g AI / ha, more preferably 40 to 400 g AI / ha.

[0029] The method for controlling sugar beet leaf spot of the present invention is to apply the composition for controlling sugar beet leaf spot two or more times, and all the application intervals may be intervals of one month or more than two months. Therefore, it is preferable to perform seed treatment or seedbed perfusion as the initial control, and the interval from seed treatment or seedbed perfusion to foliage spraying is about 2 to 3 months, or the interval between foliage spraying and the next foliage spraying is about one month. Since such a long application interval can be set, the application time can be adjusted without competition with other crop species and farming operations. In one aspect, the present invention relates to a method for controlling sugar beet leaf spot, which comprises treating seeds with isothianil and treating plants with isothianil at intervals of one month or more. In one aspect, the present invention relates to a method for controlling sugar beet leaf spot, which comprises treating soil with isothianil before sowing and / or at the time of sowing and treating plants with isothianil at intervals of one month or more. In one aspect, the present invention relates to the use of isothianil for the treatment and / or prevention of sugar beet leaf spot.

[0030] In the present invention, the control effect of the composition for controlling sugar beet brown spot disease refers to an increase in the number of sugar beet leaves, plant height elongation, stem thickening, root generation and / or activation of growth (promotion of rooting, increase in elongation amount or early rooting time), enhancement of sugar beet vitality, reduction of withering of lower leaves, improvement of root sugar content, etc. in the treated plot where the composition is applied, compared with the untreated plot where the composition is not applied, and ultimately leads to improvement of crop quality and / or increase in yield.

[0031] Hereinafter, the present invention will be further described by way of examples, but the present invention is not limited thereto.

Examples

[0032] [Test Example 1] [Preventive effect of isothianil flowable concentrate (SC) on sugar beet seedling pots (seedbeds) by perfusion and foliar spraying in the field against brown spot disease] Sugar beet variety "Ascend" (brown spot disease resistance: weak) sown in seedling pots was transplanted to the field 48 days after sowing (row spacing 66 cm × plant spacing 20 cm). The perfusion test on the seedling pots was carried out at 35.6 m 2 (without replication), and the spraying test was carried out at 8.9 m 2 (3 replications). As test chemicals, isothianil SC (isothianil 18.3% wettable powder), Flint (registered trademark) Flowable 25 (trifloxystrobin 25.0% wettable powder), and Green Pencozeb wettable powder (mancozeb 80.0% wettable powder) were used.

[0033] Each test plot is as follows. · Example 1: A treatment plot where 3 L / m 2 of 75-fold diluted (2.7 g / L) isothianil SC was perfused into the seedling pots 47 days after sowing (1 day before transplantation) (equivalent to a chemical amount of 160 g AI / ha in the main field). · Example 2: A treatment plot where 500-fold diluted (0.4 g / L) isothianil SC was foliar sprayed 55 days after transplantation (equivalent to a chemical amount of 400 g AI / ha in the main field). · Example 3: A treatment plot (dose: equivalent to 200 g AI / ha) where isotianil SC diluted 1,000-fold (0.2 g / L) was sprayed on the foliage 55 days after transplantation. · Comparative Example 1: A treatment plot where a 600-fold diluted green penconazole wettable powder was sprayed on the foliage 55 days after transplantation and Flint (registered trademark) Flowable 25 diluted 1,500-fold was sprayed on the foliage 86 days after transplantation. · Comparative Example 2: An untreated plot.

[0034] Note: In order to promote the occurrence of sugar beet cercospora leaf spot, 74 days after transplantation, a handful (about 3 g) of dry powder of diseased leaves from the previous year's production was placed at the boundary of each plot, and the sugar beet cercospora leaf spot pathogen was inoculated. Disease incidence surveys were conducted 74 days, 91 days, and 105 days after transplantation. Also, the presence or absence of phytotoxicity was determined by visually observing the above-ground parts of the sugar beet plants.

[0035] <Disease index> The disease index was determined according to the following criteria. 0: No lesions 1: Scattered lesions are seen on mature leaves 2: Lesions occur on most of the mature leaves, and large lesions are also mixed 3: Lesions occur on almost the entire surface of mature leaves, and necrosis is partially observed 4: Almost dead mature leaves are observed 5: Most of the mature leaves are dead, and the emergence of new leaves is prominent

[0036] <Calculation of disease severity> The disease severity was calculated by the following formula. That is, the percentage was shown as the ratio of the sum of the values obtained by multiplying the number of plants (the number of plants for that index) determined for each index (the index) by the value obtained by multiplying the maximum disease index (the maximum index) among the surveyed plants by the total number of surveyed plants (the number of surveyed plants).

Equation

[0037] <Calculation of control value> The control value was calculated by the following formula. That is, the ratio of the difference between the disease incidence in the untreated plot and the disease incidence in the treated plot (said disease incidence) to the value of the disease incidence in the untreated plot was shown as a percentage.

Number

[0038] The results are shown in Table 1 and Figures 1 to 3.

Table 1

[0039] <Disease status> In Comparative Example 1, the disease incidence was 23.3 at 105 days after transplantation, and 80% of the plants had few lesions. In Comparative Example 2, the disease incidence was 67.7 (disease incidence rate 100%, multiple occurrences) at 105 days after transplantation, and most of the plants had many lesions and plants with dead leaves were observed. In Examples 1 to 3, the disease incidence was 25 to 39 at 105 days after transplantation, the control value was 42 to 63, and clearly fewer lesions were observed compared to Comparative Example 2, and a control effect almost equivalent to that of Comparative Example 1 was recognized (Figure 2). Also, in Examples 1 to 3, clearly fewer dead leaves were observed at 113 days after transplantation compared to Comparative Example 2. Figure 3 shows a typical example of the disease status of Example 1 and Comparative Example 2 at 113 days after transplantation. No phytotoxicity was observed in Examples 1 to 3, and it was clarified that isothianil SC showed an excellent control effect against sugar beet leaf spot.

[0040] Also, as shown in Example 1, from the perfusion of isothianil SC into the seedling pots to 105 days after transplantation (the day of disease incidence investigation) when more than 3 months had passed, a high control effect was maintained without additional chemical treatment. Therefore, it was shown that the composition for controlling sugar beet leaf spot of the present invention can obtain an extremely long-term control effect with a single application compared to the conventional control composition that needed to repeat the application 4 to 8 times at intervals of about 10 to 15 days. Furthermore, as shown in Examples 2 and 3, a high control effect was sustained for over one month even in foliar spraying. Also, no phytotoxicity was observed in Examples 2 and 3.

[0041] [Test Example 2] [Preventive Effect of Brown Spot Disease by the Combination of Isothianil Flowable Concentrate (SC) Drenching in Sugar Beet Seedling Pots and Foliar Spraying of Fungicides in the Field] The sugar beet variety "Ascend" (weak resistance to brown spot disease) sown in seedling pots was transplanted to the field 58 days after sowing (row spacing 66 cm × plant spacing 20 cm). The drenching test in seedling pots was conducted on 35.6 m 2 (without replication), and the spraying test was conducted on 2.64 m 2 (3 replications). The test chemicals used were Isothianil SC (Isothianil 18.3% wettable powder), Flint (registered trademark) Flowable 25 (Trifloxystrobin 25.0% wettable powder), and Green Mancozeb wettable powder (Mancozeb 80.0% wettable powder).

[0042] Each test plot was as follows. · Example 4: A treatment plot where 3 L / m 2 of 1000-fold diluted (0.2 g / L) Isothianil SC was drenched in seedling pots 57 days after sowing (1 day before transplantation) (equivalent to a chemical amount of 12 g AI / ha in the main field). · Example 5: A treatment plot where 3 L / m 2 of 10,000-fold diluted (0.02 g / L) Isothianil SC was drenched in seedling pots 57 days after sowing (1 day before transplantation) (equivalent to a chemical amount of 1.2 g AI / ha in the main field). · Example 6: A treatment plot where 3 L / m 2 of 1000-fold diluted (0.2 g / L) Isothianil SC was drenched in seedling pots 57 days after sowing (1 day before transplantation), and on July 27, 1500-fold diluted Flint (registered trademark) Flowable 25 was foliarly sprayed (chemical amount: equivalent to 12 g AI / ha). · Example 7: A treatment plot where 3 L / m 2The treatment plot where the seedling-raising pots were irrigated and Flint (registered trademark) Flowable 25 diluted 1,500 times was sprayed on the stems and leaves 70 days after transplantation (dosage: equivalent to 1.2 g AI / ha). · Comparative Example 3: The treatment plot where the stems and leaves were sprayed with Green Pencozeb wettable powder diluted 600 times 49 days and 60 days after transplantation, and Flint (registered trademark) Flowable 25 diluted 1,500 times 70 days after transplantation. · Comparative Example 4: Untreated plot.

[0043] In addition, in order to promote the occurrence of sugar beet brown spot, diseased plants (1 plant per 6 plots) were transplanted into the field 69 days after transplantation. The disease incidence was investigated 73 days, 82 days, 102 days, and 109 days after transplantation. Also, the presence or absence of phytotoxicity was determined by visually observing the above-ground parts of the sugar beet plants. Similar to Test Example 1, the disease index was determined and the disease severity was calculated. The results are shown in Table 2 and Figure 4.

[0044]

Table 2

[0045] <Disease status> In Comparative Example 3, the disease severity was 22.7 at 102 days after transplantation, and more than 80% of the plants had few lesions. In Comparative Example 4, the disease severity was 46.7 (disease incidence rate 100%, medium occurrence) at 102 days after transplantation, and most of the plants had medium and many lesions. In Example 4, at 102 days after transplantation, the lesions were clearly fewer compared to Comparative Example 4, and a practical control effect was recognized. Furthermore, it showed a disease severity almost the same as that of Comparative Example 3. In Example 4, a high control effect was recognized even after more than 3 months had passed since the seedling-raising pot irrigation without additional chemical treatment. Therefore, it was shown that the composition for controlling sugar beet brown spot of the present invention can obtain a very long-term control effect with a single application compared to the conventional control composition that needed to be repeatedly applied 4 to 8 times at intervals of about 10 to 15 days. In Example 5, a control effect as good as that of Comparative Example 3 was not obtained, but a control effect was recognized compared to Comparative Example 4.

[0046] In Examples 6 and 7, which combined the seeding pot perfusion of isothianil SC and the foliar spraying of Flint (registered trademark) Floable 25, the disease incidence could be reduced to the same level as or better than that in Comparative Example 3, and a more excellent control effect was obtained. No phytotoxicity was observed in Examples 4 to 7, and it was revealed that isothianil SC also exhibited an excellent control effect against sugar beet leaf spot.

[0047] [Test Example 3] [Preventive Effect of Isothianil SC on Sugar Beet Leaf Spot by Seeding Pot Perfusion in the Field] The sugar beet variety "Anji" (strong resistance to leaf spot) sown in seeding pots was transplanted to the field 46 days after sowing (row spacing 66 cm × plant spacing 20 cm). The seeding pot perfusion test was conducted on 35.6 m 2 (with 3 replicates). The test chemicals used were isothianil SC (isothianil 18.3% wettable powder), Hoagard (registered trademark) emulsion (tetraconazole 15.0% emulsion), and Green Pencozeb wettable powder (mancozeb 80.0% wettable powder).

[0048] Each test plot was as follows. · Example 8: A treatment plot where 3 L / m of 400-fold diluted isothianil SC was used for seedbed perfusion 46 days after sowing (the day of transplantation) (the chemical dosage in the main field corresponded to 30 g AI / ha). 2 · Example 9: A treatment plot where 3 L / m of 300-fold diluted isothianil SC was used for seedbed perfusion 46 days after sowing (the day of transplantation) (the chemical dosage in the main field corresponded to 40 g AI / ha). 2 · Example 10: A treatment plot where 3 L / m of 200-fold diluted isothianil SC was used for seedbed perfusion 46 days after sowing (the day of transplantation) (the chemical dosage in the main field corresponded to 60 g AI / ha). 2 · Example 11: A treatment plot where 3 L / m of 150-fold diluted isothianil SC was used for seedbed perfusion 46 days after sowing (the day of transplantation) (the chemical dosage in the main field corresponded to 80 g AI / ha). 2 · Example 12: On the 46th day after sowing (the day of transplantation), the isothianil SC diluted 100-fold was used for seedbed irrigation at 3 L / m 2 in the treatment plot (equivalent to a chemical dosage of 120 g AI / ha in the main field). · Comparative Example 5: A treatment plot where the green penta zineb wettable powder diluted 500-fold was sprayed on the stems and leaves 57 days after transplantation, the Hoegard (registered trademark) emulsion diluted 1,500-fold and the green penta zineb wettable powder diluted 500-fold were sprayed 73 days after transplantation. · Comparative Example 6: Untreated plot.

[0049] In addition, in order to promote the occurrence of the sugar beet leaf spot pathogen, diseased plants (1 plant in every 6 plots) were transplanted into the field 78 days after transplantation. The disease incidence investigation was carried out 83 days after transplantation. In addition, the presence or absence of phytotoxicity was determined by visually observing the above-ground part of the sugar beet plants.

[0050] <Disease index> The disease index was determined according to the following criteria. 0: No lesions 0.5: 1 to 5 lesions can be observed on mature leaves 1: Lesions are scattered on mature leaves 2: Lesions occur on most of the mature leaves, and large lesions are also mixed 3: Lesions occur on almost the entire surface of mature leaves, and partial necrosis is observed 4: Almost dead mature leaves can be observed 5: Most of the mature leaves are dead, and the emergence of new leaves is prominent Except for using the above criteria, the disease incidence and control value were calculated in the same manner as in Test Example 1. The results are shown in Table 3.

[0051]

Table 3

[0052] <Disease status> Control effects were observed in all of Examples 8 to 12. No phytotoxicity was observed at the time point 78 days after transplantation, and it was clarified that isothianil SC also showed excellent control effect against sugar beet leaf spot.

[0053] [Test Example 4] [Preventive Effect of Tuber Rot of Sugar Beet by Isothianil Seed Treatment] Fifteen seeds of the sugar beet variety "Amahomare" (medium resistance to leaf spot) were sown per planter, thinned after the cotyledons had developed, and four plants per planter were left. Seventy-three days after sowing when six to seven true leaves had developed, the plants were inoculated with the leaf spot pathogen. The leaf spot pathogen spores collected from diseased sugar beet leaves with leaf spot were diluted with tap water to prepare a spore suspension of 1×10 4 cells / mL, and approximately 33 mL per planter was spray-inoculated. For three weeks after inoculation, each planter was covered with vinyl at night to create a humid condition.

[0054] Each test plot was as follows. · Example 13: A treatment plot with a seed treatment concentration of 6 g / kg of seed with isothianil (equivalent to a chemical amount of 15 g AI / ha in the field). · Example 14: A treatment plot with a seed treatment concentration of 12 g / kg of seed with isothianil (equivalent to a chemical amount of 30 g AI / ha in the field). · Example 15: A treatment plot with a seed treatment concentration of 18 g / kg of seed with isothianil (equivalent to a chemical amount of 45 g AI / ha in the field). For the seed treatments in Examples 13 to 15, isothianil SC (isothianil 18.3% wettable powder) was diluted with tap water to a predetermined concentration, and 640 μL of the diluted solution per 100 "Amahomare" pellet seeds was sprayed and applied with a spray gun, and then air-dried. · Comparative Example 7: A treatment plot where a 400-fold dilution of green pentachloronitrobenzene wettable powder in an amount equivalent to 1,000 L / ha was sprayed on the stems and leaves 3 hours before inoculation with the leaf spot pathogen. · Comparative Example 8: A test plot using untreated pellet seeds.

[0055] Thirty-one days after inoculation (104 days after transplantation), the number and area of lesions on four diseased leaves were investigated. The measurement of the lesion area was carried out for Example 13 and Comparative Example 8. For 20 lesions randomly selected from each treatment plot per strain, the measurement was performed using a digital microscope VHX-5000 (manufactured by Keyence Corporation). The ZS20 lens was used, and observations and measurements were made on 80 lesions in total from each treatment plot at a magnification of 20 times. The control value for each treatment plot was calculated from the number of lesions per leaf. The control value was calculated by using the number of lesions as the "disease incidence" in the control value calculation formula described in Test Example 1. The results are shown in Table 4, Figures 5 and 6.

[0056]

Table 4

[0057] <Disease status> In Comparative Example 7, no lesions occurred, and a high control effect was observed. In Comparative Example 8, severe occurrence was observed. In Example 13, more small lesions were observed than in Comparative Example 8 (Figure 5). The area per lesion was, on average for 80 lesions, 2.8 mm in Example 13 2 whereas it was 7.3 mm in Comparative Example 8 2 Figure 6 shows the frequency distribution of the area per lesion. When calculating the total lesion area per four leaves per strain, it was 82.9 mm in Example 13 2 whereas it was 642.4 mm in Comparative Example 8 2 That is, in Example 13, the number of lesions decreased and the lesions also became smaller compared to Comparative Example 8. From the total lesion area per four leaves per strain, the control value was 87 in Example 13, and practicality can be expected. Also, no phytotoxicity was observed.

[0058] In Examples 14 and 15 as well, a decrease in the number of lesions and a reduction in the size of the lesions were confirmed in the same manner as in Example 13. On the other hand, a dose-dependent control effect was not observed. From the perspective of reducing costs by suppressing the administration dose, it is considered that 6 g / kg of isothianil seed coating is suitable for seed treatment.

[0059] [Test Example 5] <Control Effect of Beet Brown Spot by Isothianil Seed Treatment> The sugar beet variety "Paprika" (brown spot resistance: slightly weak) was directly sown at a rate of 4 m 2 per unit area. As test agents, isothianil FS (isothianil 18.0% wettable powder) and green penta mancozeb wettable powder (mancozeb 80.0% wettable powder) were used.

[0060] Each test plot is as follows. · Example 16: A treatment plot where the seed treatment concentration was isothianil FS 6 g / kg of seeds and was directly sown in the field (the amount of the drug in the main field: equivalent to 15 g AI / ha). · Example 17: A treatment plot where the seed treatment concentration was isothianil FS 18 g / kg of seeds and was directly sown in the field (the amount of the drug in the main field: equivalent to 45 g AI / ha). · Example 18: A treatment plot where the seed treatment concentration was isothianil FS 36 g / kg of seeds and was directly sown in the field (the amount of the drug in the main field: equivalent to 90 g AI / ha). Note that for the seed treatments in Examples 16 to 18, 8 days before sowing, isothianil FS (20.0% isothianil) was diluted to a predetermined concentration with tap water, and 640 μL of the diluted solution per 100 pellet seeds of the sugar beet variety "Paprika" (brown spot resistance: slightly weak) was sprayed and applied with a spray gun, and then air-dried. · Comparative Example 9: A treatment plot where a 500-fold dilution of green penta mancozeb wettable powder was foliarly sprayed at an equivalent amount of 1,000 L / ha 72 days and 85 days after sowing. · Comparative Example 10: A test plot using untreated pellet seeds.

[0061] The disease investigation was conducted 89 days after sowing. Also, the presence or absence of phytotoxicity was determined by visually observing the above-ground parts of the sugar beet plants. Similar to Test Example 4, the disease index was determined, and the disease severity and control value were calculated. The results are shown in Table 5.

[0062]

Table 5

[0063] <Incidence status> In any of Examples 16 to 18, a tendency for the incidence to be reduced compared to Comparative Example 10 was observed. Also, no phytotoxicity was observed.

[0064] <Amount of active ingredient per 1 ha of field (g AI / ha)> In each of the above Examples, the amount of active ingredient (chemical amount) per 1 ha of field was calculated as follows. · Seed treatment: It was calculated assuming that the number of pelleted seeds sown per 1 ha of field was 84,000 seeds and the 1000-seed weight of the pelleted seeds was 30 g. · Seedbed irrigation: The irrigation amount of the seedling box was 3 L / m 2 and the area of the seedling box per 1 ha of field was 20 m 2 That is, it was calculated that the irrigation amount of the seedling box per 1 ha of field was 60 L. · Foliar spraying: It was calculated that the spraying amount per 1 ha of field was 1,000 L.

Industrial applicability

[0065] The present invention can provide a composition for controlling sugar beet leaf spot containing isothianyl, which has a low risk of generating drug-resistant bacteria, etc., a long persistence of the control effect, and a labor-saving treatment, and a method for controlling sugar beet leaf spot using the composition for controlling the same disease. Thereby, it can be used for the prevention and control of sugar beet leaf spot, which causes great damage in sugar beet cultivation.

Claims

1. A composition for controlling sugar beet leaf spot disease containing isothianyl.

2. The composition according to claim 1, further comprising one or more fungicides and / or insecticides other than isothianyl.

3. The fungicide is selected from the group consisting of phenylamide fungicides, aromatic heterocyclic fungicides, methyl benzimidazole carbamate (MBC) fungicides, N-phenylcarbamate fungicides, thiazole carboxamide fungicides, phenylurea fungicides, benzamide fungicides, pyrimidineamine fungicides, pyrazole-MET1 fungicides, succinate dehydrogenase inhibitors (SDHI), QoI fungicides, QiI fungicides, QoSI fungicides, oxidative phosphorylation uncouplers, anilinopyrimidine fungicides, hexopyranosyl antibiotics, glucopyranosyl antibiotics, tetracycline antibiotics, phenylpyrrole fungicides, dicarboximide fungicides, phosphorothiolate fungicides, dithiolane fungicides, AH fungicides, carbamate fungicides, microbial pesticides, oxysterol binding protein inhibitors (OSBPI), demethylation inhibitors (DMI fungicides) (SBI: Class I), SBI (Class III) fungicides, CAA fungicides, MBI-R fungicides, MBI-D fungicides, MBI-P fungicides, benzisothiazole fungicides, thiadiazole carboxamide fungicides, cyanoacetamide = oxime fungicides, phosphonate fungicides, benzenesulfonic acid fungicides, phenylacetamide fungicides, allylphenylketone fungicides, thiazolidine fungicides, pyrimidinone hydrazone fungicides, 4-quinolylacetic acid fungicides, tetrazolyloxime fungicides, copper agents, dithiocarbamate fungicides, phthalimide fungicides, chloronitrile fungicides and bisguanidine fungicides, and the composition according to claim 2 is one or more selected from the group.

4. The composition according to claim 2 or 3, wherein the insecticide is one or more selected from the group consisting of acetylcholinesterase (AChE) inhibitors, GABA-activated chloride ion channel blockers, sodium channel modulators, nicotinic acetylcholine receptor (nAChR) competitive modulators, nicotinic acetylcholine receptor (nAChR) allosteric modulators, glutamate-activated chloride ion channel (GluCl) allosteric modulators, juvenile hormone analogs, chordotonal organ TRPV channel modulators, mitochondrial ATP synthase inhibitors, oxidative phosphorylation uncouplers that disrupt the proton gradient, nicotinic acetylcholine receptor (nAChR) channel blockers, chitin biosynthesis inhibitors, octopamine receptor agonists, mitochondrial electron transport system complex III inhibitors, mitochondrial electron transport system complex I inhibitors (METI), voltage-dependent sodium channel blockers, acetyl-CoA carboxylase inhibitors, mitochondrial electron transport system complex II inhibitors, ryanodine receptor modulators, and chordotonal organ modulators.

5. A method for controlling sugar beet leaf spot, comprising applying a composition for controlling sugar beet leaf spot containing isothianil to sugar beet.

6. The method according to claim 5, wherein the application is to at least one of the sugar beet plant, the culture soil of the plant, and the seeds configured to produce the plant.

7. The method according to claim 5 or 6, wherein the application is performed two or more times.

8. The method according to any one of claims 5 to 7, wherein the composition for controlling sugar beet leaf spot containing isothianil is the composition according to any one of claims 1 to 4.

9. A method for controlling sugar beet leaf spot, characterized in that the seedbed is treated with isothianil before sowing and / or at the time of sowing.

10. A method for controlling sugar beet leaf spot, characterized by treating seeds with isothianil and treating the plant body with isothianil at intervals of one month or more.

11. A method for controlling sugar beet leaf spot, characterized by treating the soil with isothianil before sowing and / or at the time of sowing and treating the plant body with isothianil at intervals of one month or more.

12. Use of isothianil for the treatment and / or prevention of sugar beet leaf spot.

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