Optical Measurement Device

Abstract

The present invention realizes an optical measurement device 202 that suppresses the influence of thermal radiation on sample temperature adjustment mechanisms 403, 404, 405 for adjusting the temperature inside a dark box 207 in which a sample is arranged, is able to efficiently maintain a low temperature inside the dark box 207 while maintaining a light-blocking effect, and is able to detect bioluminescence / chemiluminescence of a substance with high sensitivity and high precision. This optical measurement device 202 comprises: a detector 406 that has a light-receiving unit 407 that receives light generated by a sample tube 803 arranged inside the dark box 207, and detects the generated light; a temperature adjustment block 401 that is arranged in the periphery of the detector 406, a portion of the temperature adjustment block 401 being arranged inside the dark box 207 and the other portion thereof being arranged outside the dark box 207; and the sample temperature adjustment mechanisms 403, 404, 405 that are connected to the portion of the temperature adjustment block 401 arranged outside of the dark box 207.

Description

[Technical Field]

[0001] The present invention relates to an optical measurement device for detecting chemiluminescence or bioluminescence of a substance contained in a liquid sample with high sensitivity and accuracy. [Background technology]

[0002] In pharmaceutical and beverage factories, products are manufactured in manufacturing facilities that provide a sterile environment.

[0003] Traditionally, culture methods have been used in microbial testing to ensure the sterility of manufacturing facilities and products. In culture methods, a sample is added to a medium and cultured at a high temperature for several days to 14 days, and the number of colonies of bacteria that grow is counted visually. Therefore, it takes time to obtain test results, and the test results must be waited for before the product is shipped.

[0004] Given this background, there is a need for the development of a rapid microbial testing method that can quickly determine sterility.

[0005] One of the quick and easy methods for microbial testing is the adenosine triphosphate (ATP) bioluminescence method (hereafter referred to as the ATP method). The ATP method uses the luminescence reaction of fireflies to convert the ATP present in bacteria into light and measure it.

[0006] More specifically, luciferin and ATP molecules are incorporated into luciferase, and the amount of light emitted when the oxidized luciferin (oxyluciferin) transitions from an excited state to a ground state as ATP is consumed is measured.

[0007] At this time, photons are generated as ATP molecules are consumed. The number of photons generated is proportional to the number of ATP. ATP molecules exist as an energy source in live bacteria, and by measuring the amount of light emitted by ATP in the sample, the total number of live bacteria can be estimated.

[0008] The luciferin-luciferase reaction has the highest quantum efficiency (ΦBL: ≈0.5) among bioluminescence and chemiluminescence, and therefore can detect hundreds of thousands of photons from a single cell.

[0009] Therefore, the ATP method is, in principle, a method that can detect light equivalent to that of a single cell.

[0010] However, in order to detect extremely small amounts (a few bacteria) of bacteria contaminating a specimen with high sensitivity and accuracy, it is necessary to increase the sensitivity of the luminescence measuring device.

[0011] The luminescence coefficient using the ATP method is measured using a reagent kit from a certain manufacturer, and the measurement procedure can be briefly explained as follows.

[0012] 1) Dispense ATP elimination solution into the sample solution to eliminate ATP in the solution and dead bacteria other than live bacteria. 2) Dispense ATP extract into the sample solution to extract ATP from live bacteria. 3) Add the luminescence reagent to the sample solution. 4) Measure the amount of light emitted.

[0013] To increase the sensitivity of luminescence measurement, it is necessary to keep the temperature of the detector low to reduce noise from the measuring equipment, and to make the area holding the measuring equipment a light-shielded space to eliminate the effects of ambient light.

[0014] Furthermore, since luminescence occurs immediately after dispensing the luminescent reagent into the sample container, in order to accurately measure the amount of ATP, it is desirable to perform the processes from 3) onwards with the sample placed in the detector.

[0015] It is common to eliminate the effects of ambient light by covering the photodetector and sample container with a light-shielding structure, but it is also effective to locally shield the luminescence measuring instrument from light, as is the case with lumimeters and by installing an open / close shutter in front of the light-receiving surface of the photodetector.

[0016] On the other hand, it is also necessary to prevent contamination from ATP and bacteria present in the environment, microorganisms carried by people, dust that has adsorbed ATP, etc. It is important to automate the dispensing of reagents within the device, transporting samples to the measurement position, and other human operations as much as possible, as well as to minimize air circulation within the storage area.

[0017] Patent Document 1 describes a technology that uses a shutter that is normally closed and opens when luminescence measurement is being performed, preventing external light from entering the luminescence detection means when luminescence measurement is not being performed, thereby preventing deterioration.

[0018] Furthermore, Patent Document 2 describes a technique in which, in preparation for measurement, a shutter unit is closed to block stray light from entering the photodetector, and in measurement, the shutter unit is opened. [Prior art documents] [Patent documents]

[0019] [Patent Document 1] Japanese Patent Application Publication No. 7-83831 [Patent Document 2] Japanese Patent Application Laid-Open No. 2008-268019 Summary of the Invention [Problem to be solved by the invention]

[0020] In an analyzer that counts the number of photons using a photodetector, it is necessary to suppress the dark level of the light receiving section below a certain level in order to increase detection sensitivity.

[0021] In general, in order to suppress the dark level of a photodetector, it is necessary to place the light receiving section in a dark box structure and to adjust the temperature to a low level.

[0022] If the temperature control mechanism of the photodetector is placed inside the dark box structure, the temperature inside the dark box structure will rise due to heat dissipation caused by the temperature control, which will cause problems such as a decrease in the efficiency of the enzyme reaction.

[0023] The technologies described in Patent Documents 1 and 2 teach light blocking, but make no mention of maintaining a low temperature inside the dark box, and do not recognize or take into consideration the problem of reduced efficiency of enzyme reactions due to heat dissipation by the temperature control mechanism.

[0024] The object of the present invention is to realize an optical measurement device that can suppress the effects of heat radiation from the temperature control mechanism that controls the temperature inside the dark box in which the sample is placed, efficiently maintain a low temperature inside the dark box while maintaining light-blocking properties, and detect the bioluminescence and chemiluminescence of substances with high sensitivity and accuracy. [Means for solving the problem]

[0025] In order to achieve the above object, the present invention is configured as follows.

[0026] In an optical measurement device comprising a detector having a light receiving unit that receives light emitted from a sample tube placed in a dark box and detects the emitted light, a temperature control block that is placed around the detector, and a sample temperature control mechanism that is connected to the temperature control block, a part of the temperature control block is placed inside the dark box and another part is placed outside the dark box, and the sample temperature control mechanism is connected to the part of the temperature control block that is placed outside the dark box. The temperature control block has a sample tube holder that holds the sample tube in the dark box. . [Effects of the Invention]

[0027] According to the present invention, it is possible to suppress the effects of heat radiation from the temperature control mechanism that controls the temperature inside the dark box in which the sample is placed, efficiently maintain a low temperature inside the dark box while maintaining light-blocking properties, and realize an optical measurement device that can detect the bioluminescence and chemiluminescence of substances with high sensitivity and accuracy. [Brief explanation of the drawings]

[0028] [Figure 1] 1 is a diagram showing the overall configuration of a rapid microorganism testing device according to an embodiment. [Figure 2]1 is a diagram showing the overall configuration of a rapid microorganism testing device according to an embodiment. [Figure 3] 1 is a diagram showing a schematic configuration of the inside of a rapid microorganism testing device according to an embodiment. [Figure 4] FIG. 2 is a diagram showing a schematic configuration of a photodetector unit according to an embodiment. [Figure 5] FIG. 2 is a schematic diagram of a sample tube transport mechanism. [Figure 6] FIG. 2 is a diagram showing a schematic configuration of a reagent dispensing machine. [Figure 7] FIG. 2 is a diagram showing a schematic configuration of a reagent rack. [Figure 8] FIG. 2 is a diagram showing an outline of a light-shielding structure of a photodetector unit. [Figure 9] FIG. 2 is a diagram showing a schematic configuration of a light receiving section of a photodetector unit according to an embodiment. [Figure 10] 1 is a flowchart illustrating a typical example of luminescence measurement in an embodiment. [Figure 11] FIG. 10 is a diagram showing a schematic configuration of a modified example of the photodetector unit according to the embodiment. DETAILED DESCRIPTION OF THE INVENTION

[0029] Hereinafter, an embodiment of the present invention will be described with reference to the accompanying drawings.

[0030] However, this embodiment is merely an example for realizing the present invention and does not limit the present invention. Furthermore, the same reference numerals are used for common components in each drawing. In the following description of the examples, the term "sample" is synonymous with "specimen." [Example]

[0031] 1 is a diagram showing the overall configuration of a rapid microorganism testing device 100 having an optical measurement unit 202 (described later) which is an optical measurement device according to one embodiment. The rapid microorganism testing device 100 is a system having a control PC 103, and power is supplied to the system via a power cable 101. The rapid microorganism testing device 100 is connected to the control PC 103 via a communication cable 102.

[0032] The rapid microorganism testing device 100 is equipped with a system water drawer 105 for loading system water for feeding liquid and cleaning the dispenser, a waste liquid drawer 104 for accessing the waste liquid section into which waste water for cleaning the dispenser flows, a reagent door 106 for loading a luminescent reagent into the rapid microorganism testing device 100, and a sample door 107 for loading a sample tube 803 (shown in FIG. 8) into the rapid microorganism testing device 100. Although not shown, each door is an open / close door with a locking mechanism, or is configured to be able to be pulled out using slide rails or the like.

[0033] FIG. 2 is a diagram showing the general configuration of the rapid microorganism testing device 100. As shown in FIG.

[0034] 2, the rapid microorganism testing device 100 comprises a dark box 207 whose interior is a dark box structure due to its light-blocking structure, and a housing 108 that surrounds the portion that does not have the dark box structure. The dark box 207 comprises a front panel 206 that is equipped with a reagent door 106 and a sample door 107. The reagent door 106 and the sample door 107 form a light-blocking structure when closed. The reagent door 106 and the sample door 107 also serve as outside air intake sections that take in outside air.

[0035] Although not shown, the door portion with a light-blocking structure has a concave-convex structure and is black anodized at the door opening. Also, a resin packing is sandwiched around the periphery of the front panel 206, which provides a light-blocking structure between the front panel 206 and the housing, forming a dark box 207.

[0036] On the other hand, the system water drawer 105, waste liquid drawer 104, liquid supply syringe 204, and the control board, power supply, etc. required for device operation are arranged outside the structure of the dark box 207. The piping from the liquid supply syringe 204 to the inside is connected by a bulkhead piping 205.

[0037] Although not shown in the figure, the harness connection to the dark box 207 is a light-shielding connection board 201, which is a board with a relay connector, and by sandwiching a light-shielding packing, a light-shielding connection is made, connecting the board and power supply located outside the dark box structure to the unit located inside the dark box.

[0038] Measurement reagents are set in a reagent rack 203 through a reagent door 106. The reagent rack 203 is equipped with a reagent temperature control mechanism 805, as will be described later, and is temperature-controlled to a constant temperature. Sample tubes 803 are placed in a tube rack and set in a sample rack mechanism 301 (shown in FIG. 3) through a sample door 107.

[0039] FIG. 3 is a diagram showing a schematic configuration of the inside of the rapid microorganism testing device 100. As shown in FIG.

[0040] In Figure 3, the rapid microorganism testing device 100 is equipped with a reagent rack 203 for storing reagents, a photodetector unit 202 for performing measurements, a sample rack mechanism 301 for holding sample tubes 803 containing samples, a chuck arm 303 for transporting the tube rack inside the dark box 207, and a dispenser 302 for dispensing reagents.

[0041] Generally, a photomultiplier tube (hereinafter referred to as PMT) is used as the photodetector unit 202, which enables highly sensitive detection.

[0042] However, if sensitivity as high as that of a PMT is not required, a semiconductor element such as a photodiode can be used. This specification will be described with reference to the case where a PMT is used.

[0043] The rapid microorganism testing device 100 includes a chuck arm 303 for transporting a sample tube 803 set in a sample rack mechanism 301 .

[0044] 5 is a schematic diagram of the sample tube transport mechanism, in which a sample tube 803 is gripped by a chuck arm 303 and transported to the photodetector unit 202 as a measurement target.

[0045] Although not shown, the sample rack mechanism 301 is equipped with a Y-drive unit that can be driven in the Y-axis direction, and these drive mechanisms make it possible to process multiple sample tubes 803. As the drive unit of the sample rack mechanism 301, for example, an actuator or a belt-pulley drive mechanism can be used.

[0046] The chuck arm 303 includes a tube gripping portion 504 for gripping the sample tube 803, and a chuck opening / closing mechanism 503 for opening and closing the tube gripping portion 504. As the chuck opening / closing mechanism 503, for example, an opening / closing mechanism using the rotation of a rotary solenoid or an opening / closing mechanism using a push solenoid can be used.

[0047] Although not shown, chuck gripping section 504 has two or more claws, and is driven by chuck opening / closing mechanism 503 to grip sample tube 803. Chuck arm 303 also has X drive section 501 that can be driven in the X-axis direction and Z drive section 502 that can be driven in the Z direction, and by driving in the X and Z directions, sample tube 803 placed in the tube rack is transported to photodetector unit 202. The drive system for chuck arm 303 can be, for example, an actuator or a belt-pulley drive mechanism.

[0048] FIG. 6 is a diagram showing a schematic configuration of a reagent dispensing machine.

[0049] 6, a dispensing nozzle 603 is used to aspirate a reagent and dispense it into a sample tube 803. The dispensing nozzle 603 is connected to a syringe 604, solenoid valves 606 and 607, and system water 605 via piping 608. The dispensing nozzle 603 is equipped with an X drive unit 602 that can be driven in the X-axis direction and a Z drive unit 601 that can be driven in the Z direction, and by driving in the X and Z directions, the nozzle performs a dispensing operation from a reagent mounted on a reagent rack 203 to a sample tube 803 placed in the photodetector unit 202.

[0050] The drive system can be, for example, an actuator or a belt-pulley drive mechanism. Although the dispenser is not shown, the dispenser nozzle 603 moves to a washing port, where the inside and outside of the dispenser nozzle 603 are washed using system water 605. The waste liquid after washing is discharged through piping into a waste liquid bottle installed in a waste liquid drawer 104 located outside the dark box 207.

[0051] FIG. 7 is a diagram showing a schematic configuration of the reagent rack 203.

[0052] 7, the reagent rack 203 is composed of a rack 701 that holds reagent holders 703 that support reagent tubes 702, and a reagent temperature adjustment mechanism 805. The temperature of the rack 701 is adjusted to a constant temperature by the reagent temperature adjustment mechanism 805. An example of the configuration of the temperature adjustment mechanism 805 is shown in Fig. 7. The reagent temperature adjustment mechanism 805 is equipped with a Peltier element 710, a heat sink 707, a heat dissipation block 709, and an air-cooling fan 708.

[0053] Rack 701 of the reagent rack 203 is placed in the dark box 207. On the other hand, a heat sink 707 for heat dissipation and an air-cooling fan 708 are placed outside the structure of the dark box 207. A light-shielding block 706 is installed between the inside of the dark box 207 and the external heat sink 707, and shields the dark box 207 from light. The light-shielding block 706 has a light-shielding member 705 and an intermediate plate 704 placed inside the dark box 207.

[0054] FIG. 4 is a diagram showing a schematic configuration of the photodetector unit 202. As shown in FIG.

[0055] In FIG. 4, the photodetector unit 202 includes a detector 406, a temperature control block 401 arranged to surround the detector, a light-shielding connection part 402, and a sample temperature control mechanism (a cooling fan 403, a heat sink 404, and a Peltier element 405).

[0056] The light receiving unit 407 of the detector 406 is disposed inside the dark box 207 so as to face the sample tube 803, and the sample temperature control mechanism is disposed outside the structure of the dark box 207. An example of the configuration of the sample temperature control mechanism is shown in Fig. 4. The sample temperature control mechanism includes a Peltier element 405, a heat sink 404, and an air-cooling fan 403, and controls the temperature control block 401 surrounding the detector 406 to a constant temperature.

[0057] The dark box 207 is surrounded by a housing 108 having an outside air intake section, and the temperature control block 401 is arranged across the dark box 207 and the inside of the housing 108, which is outside the dark box 207.

[0058] FIG. 8 is a diagram showing an outline of the light-shielding structure in the photodetector unit 202. As shown in FIG.

[0059] 8, the light-shielding connection part 402 has a light-shielding structure by sandwiching a light-shielding member 801 in an opening provided in a middle plate 704 constituting the dark box 207. The connection part between the light-shielding connection part 402 and the temperature control block 401 also has a light-shielding structure by sandwiching a light-shielding member 801. The light-shielding member 801 can be made of a resin material, packing, or the like. Furthermore, other than a structure in which the light-shielding member 801 is sandwiched, the part where the light-shielding connection is made can also be made into an uneven structure to block light.

[0060] 9 shows a schematic configuration of the light receiving section of the photodetector unit 202. The temperature control block 401 has a sample tube holding section 401h that holds the sample tube 803. As shown in FIG. 9, the sample tube holding section 401h has a conical internal space (conical section) formed therein, and holds the sample tube 803 in this internal space. By holding the sample tube 803 in the internal space, the temperature of the sample accommodated in the sample tube 803 can be stabilized more effectively.

[0061] The inner wall of the conical part of the sample tube holder 401h uses specular reflection to collect light scattered in a direction different from the light receiving part of the detector 406. To achieve specular reflection, a polished or plated metal material is used. Using aluminum or the like as the metal material makes it possible to obtain stable reflection efficiency.

[0062] Furthermore, the shape of the part that holds the tube is not limited to a cone, and may be semicircular or other shapes.

[0063] 9, the sample tube 803 placed on the detector 406 is placed above the light receiving section 407 of the photodetector unit 202 that performs the detection. The closer the distance between the sample tube 803 and the light receiving section 407 of the detector 406, the higher the detection sensitivity. However, the sample tube 803 is often made of plastic and may be charged with static electricity.

[0064] Therefore, there is a possibility that discharge may occur when the sample tube 803 and the light receiving unit 407 are brought close to each other. The detector 406 may not be able to perform accurate measurements due to discharge or the like.

[0065] Therefore, a glass plate 802 is placed between the detector 406 and the sample tube 803. A material with sufficient transmittance in the visible region, such as a quartz plate, is used as the glass plate 802. It is also possible to select a fluorescent filter or the like with a specific band in the wavelength region of the luminescent reaction.

[0066] FIG. 10 is a flow chart illustrating a typical example of luminescence measurement in one embodiment of the present invention.

[0067] 10, after starting the software in the rapid microorganism testing device 100 and the control PC 103, the reagent door 106 is opened (step S01), and the reagent holder 703 containing the luminescent reagent is placed in the rack 701 (step S02). After the reagent holder 703 is placed in the rack 701, the reagent door 106 is closed (step S03).

[0068] Next, the sample door 107 is opened (step S04), and the tube rack containing the tubes containing the measurement samples is placed in the sample rack mechanism 301 (step S05). After the tube rack is placed in the sample rack mechanism 301, the sample door 107 is closed (step S06). Measurement conditions are entered using the control software, and the measurement start button on the control PC 103 is pressed.

[0069] After the measurement starts, the sample tube 803 is transported from the sample rack mechanism 301 to the photodetector unit 202 by the chuck arm 303 (step S07). The luminescent reagent is dispensed from the reagent tube 702 set in the reagent holder 703 into the transported sample tube 803 by the dispenser 302 (step S08).

[0070] After the reagent is dispensed into the sample tube 803, light is emitted and the number of emitted light is measured by the detector 406 (step S09).

[0071] After measuring the number of luminescences, the sample tube 803 is transported by the chuck arm 303 to the sample rack mechanism 301 (step S10). The dispenser 302 then moves to the cleaning port and performs a cleaning operation on the dispensing nozzle 603 using system water 605 (step S11). If there are multiple samples, the next sample tube 803 to be measured is transported and luminescence measurement is performed sequentially (steps S07 to S11).

[0072] After all samples have been measured, the reagent door 106 is opened (step S12) and the luminescent reagent is removed (step S13). After the luminescent reagent is removed, the reagent door 106 is closed (step S14) and the sample door 107 is opened (step S15). The sample tube rack is removed (step S16), and then the sample door 107 is closed (step S17) to complete the process.

[0073] The acquired data on the number of luminescences is sent to the control PC 103, where it is converted from the number of luminescences to ATP concentration using a calibration curve created in advance. The software according to the present invention has multiple calibration curves, and conversion to ATP concentration is performed using a selected calibration curve, and the measurement data and calibration curve information are linked and stored.

[0074] As described above, the optical measurement device 202 according to one embodiment of the present invention has a light receiving unit 407 that receives light generated from a sample tube 803 arranged in a dark box 207, and is equipped with a detector 406 that detects the generated light, a temperature control block 401 that is arranged around the detector 406, with a part of it arranged inside the dark box 207 and the other part arranged outside the dark box 207, and temperature control mechanisms 403, 404, 405 that are connected to the part of the temperature control block 401 that is arranged outside the dark box 207.

[0075] A part of the temperature control block 401 covers the light receiving part 407 of the photodetector unit 202 inside the dark box 207, and another part of the temperature control block 401 is arranged outside the dark box 207, and the part of the temperature control block 401 arranged outside the dark box 207 is equipped with a temperature control mechanism, a cooling fan 403, a heat sink 404, and a heat sink 404.

[0076] Therefore, according to one embodiment of the present invention, the influence of heat radiation from the temperature control mechanism that controls the temperature inside the dark box 207 in which the sample is placed is suppressed, and the inside of the dark box 207 can be efficiently maintained at a low temperature while maintaining light blocking properties, and an optical measurement device that can detect bioluminescence and chemiluminescence of a substance with high sensitivity and high accuracy is provided. Place It can be realized.

[0077] Furthermore, according to one embodiment of the present invention, a Peltier element 710 that maintains a constant temperature for the rack 701 that holds the reagent holders for the reagent tubes 702 is disposed inside the dark box 207, and a heat sink 707 and a cooling fan 708 that are connected to a heat dissipation block 709 of the Peltier element 710 are disposed outside the dark box 207, so that the reagent inside the dark box 207 can be efficiently maintained at a constant temperature.

[0078] In the above-described embodiment, as shown in Figures 4, 8, and 9, the temperature control block 401 surrounds the light receiving section 407 of the photodetector unit 202. However, as shown in Figure 11, the temperature control block 401 may also be configured not to surround the light receiving section 407.

[0079] In the modification shown in Fig. 11, a sample tube support member 804 surrounds the light receiving unit 407, supports the glass plate 802, and also holds the sample tube 803. Fig. 9 shows a schematic configuration of the light receiving unit of the optical measuring instrument. The temperature control block 401 has a sample tube holder 401h that holds the sample tube 803.

[0080] 9, in the example shown in Fig. 11, a conical internal space (conical portion) is formed in the sample tube support member 804, and the sample tube 803 is held in this internal space. By holding the sample tube 803 in the internal space, the temperature of the sample accommodated in the sample tube 803 is stabilized more effectively. [Explanation of symbols]

[0081] 100 Rapid microorganism testing device, 101 Power cable, 102 Communication cable, 103 Control PC, 104 Waste liquid drawer, 105 System water drawer, 106 Reagent door (outside air intake section), 107 Sample door (outside air intake section), 108 Housing, 201 Light-shielding connection board, 202 Photodetector unit (light measurement device), 203 Reagent rack, 204... Syringe for liquid delivery, 205... Bulkhead piping, 206... Front panel, 207... Dark box, 301... Sample rack mechanism, 302... Dispenser, 303... Chuck arm, 401... Temperature control block, 401h... Sample tube holder, 402... Light-shielding connection part, 403... Air-cooling fan, 404... Heat sink, 405... Peltier element child, 406 detector, 407 light receiving unit, 501 X drive unit, 502 Z drive unit, 503 chuck opening / closing mechanism, 504 tube gripper, 601 Z drive unit, 602 X drive unit, 603 dispensing nozzle, 604 syringe, 605 system water, 606 solenoid valve 1, 607 solenoid valve 2, 608 piping, 701 rack, 702 Reagent tube, 703 Reagent holder, 704 Middle plate, 705 Light shielding member, 706 Light shielding block, 707 Heat sink, 708 Cooling fan, 709 Heat dissipation block, 710 Peltier element, 801 Light shielding member, 802 Glass plate, 803 Sample tube, 804 Sample tube support member, 805 Reagent temperature control mechanism

Claims

1. a detector having a light receiving unit that receives light emitted from a sample tube placed in a dark box and detects the emitted light; a temperature control block disposed around the detector; a sample temperature control mechanism connected to the temperature control block; In an optical measurement device comprising: a part of the temperature control block is disposed inside the dark box and another part is disposed outside the dark box, and the sample temperature control mechanism is connected to the part of the temperature control block disposed outside the dark box; The optical measurement device is characterized in that the temperature control block has a sample tube holder that holds the sample tube in the dark box.

2. 2. The optical measurement device according to claim 1, The optical measurement device is characterized in that the temperature control block is disposed in the dark box so as to cover the outer periphery of the light receiving unit.

3. 2. The optical measurement device according to claim 1, An optical measurement device characterized in that the dark box is surrounded by a housing having an outside air intake section, and the temperature control block is arranged across the dark box and inside the housing, which is outside the dark box.

4. 2. The optical measurement device of claim 1, Inside the dark box, a transport mechanism for the sample tube; An optical measurement device characterized in that a dispensing mechanism for dispensing a reagent is disposed.

5. 2. The optical measurement device of claim 1, An optical measurement device characterized in that a reagent temperature control mechanism is disposed in the dark box, a part of which is disposed inside the dark box and another part of which is disposed outside the dark box, the reagent temperature control mechanism having a Peltier element, a heat sink, a heat dissipation block, and a cooling fan, the heat sink and the cooling fan being disposed outside the dark box.

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