Cleaning compositions containing polypeptides with alpha-amylase activity

JP7747789B2Active Publication Date: 2025-10-01PROCTER & GAMBLE CO
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Patent Information

Application Number
JP2024007506
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Priority Date
2020-12-09
Filing Date
2024-01-22
Publication Date
2025-10-01
Estimated Expiration
2040-12-18

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Abstract

To provide a cleaning composition comprising an α-amylase variant exhibiting an improved property, such as removal of starch-containing stains, or specific activity, compared to a parent polypeptide.SOLUTION: A cleaning composition comprises: (i) an α-amylase variant of a parent α-amylase, the variant having a particular amino acid sequence and having an improved performance compared to the parent polypeptide; and (ii) a cleaning adjunct.SELECTED DRAWING: None
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Claims

1. 1. A cleaning composition comprising: (i) an α-amylase variant of a parent α-amylase having the amino acid sequence of SEQ ID NO: 1, wherein the α-amylase variant is selected from the group consisting of: H1 * +G7A+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180 * +T182L+G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+S181L+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180S+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+G113S+F116W+D123N+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+F116L+D123N+N126D+T131I+S181 * +T182 * +F202Y+Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+H320T+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261K+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+A383D+E390A+D405G+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +A60V+Q98L+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+A383D+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+N394W+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474L、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302T+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G475E、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474F、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+T256D+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242L+E259G+N279S+G303R+W346N+E390A+G475K、 H1 * +G7A+R37A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360Y+E390A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+R414M+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+F261P+N279S+G303R+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+H323W+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+P321Y+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360M+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+H420A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+V365I+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+N474L、 H1 * +G7A+G73L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+K93R+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390D+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+V103I+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+E390A+N474L、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+R414M+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+Q360Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+S243Q+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+L205Y+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N422V+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474F、 H1 * +N3G+G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+N470M+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420P+G476H、 H1 * +G7A+R37A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N470M+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+H420P+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+H420A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+H323W+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+R414M+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G476A、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+H420A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G361M+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3G+G7A+V104M+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+N174K+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 N3G+G7A+R37V+D118T+W140M+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321Y+L354V+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470A+G475K、 H1 * +G7A+A31S+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1* + G7A + D118T + W140Y + R180* + S181* + T182D + E189P + N194F + L205Y + T224Y + Y242F + F266Y + N279S + K280Y + G303R + E390A + G476A, and H1* + G7A + D118T + W140Y + R180* + S181* + T182S + A185Q + E189P + N194F + Y242F + E259G + N279S + K280Y + S302T + E390A + G475K, 1. A cleaning composition comprising: an α-amylase variant of a parent α-amylase, wherein the α-amylase variant has at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to SEQ ID NO: 1, and wherein the α-amylase variant has improved cleaning performance, as measured by specific activity, when compared to the parent α-amylase, by an improvement factor (IF) of >1.0; and (ii) a cleaning adjunct.

2. A cleaning composition as described in claim 1, wherein the α-amylase variant has improved cleaning performance relative to a measured specific activity of an improvement factor (IF) > 1.0 when compared to the α-amylase of sequence number 2 or 3.

3. 3. The cleaning composition of claim 1, wherein the cleaning adjunct comprises an additional enzyme.

4. The cleaning composition of claim 3, wherein the additional enzyme is selected from the group consisting of protease, amylase, lipase, catinase, cellulase, nuclease, endoglucanase, xyloglucanase, pectinase, pectin lyase, xanthanase, xanthan lyase, peroxidase, haloperoxygenase, hexosaminidase, catalase, and mannase, or any mixture thereof.

5. A cleaning composition described in any one of claims 1 to 4, wherein the cleaning composition is a liquid or solid detergent composition.

6. A cleaning composition according to any one of claims 1 to 4, wherein the cleaning composition is a laundry composition or a dishwashing composition.

7. 1. A method of making a cleaning composition, comprising: (i) combining the following sequences, using SEQ ID NO: 1 for numbering: H1* + G7A + S181* + T182* + Y242F + E259G + N279S + G303R + E390A + G475K; H1 * +G7A+R180 * +T182L+G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+S181L+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180S+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+G113S+F116W+D123N+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+F116L+D123N+N126D+T131I+S181 * +T182 * +F202Y+Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+H320T+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261K+N279S+G303R+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+A383D+E390A+D405G+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +A60V+Q98L+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+A383D+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+N394W+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474L、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302T+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G475E、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474F、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+T256D+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242L+E259G+N279S+G303R+W346N+E390A+G475K、 H1 * +G7A+R37A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360Y+E390A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+R414M+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+F261P+N279S+G303R+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+H323W+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+P321Y+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360M+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+H420A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+V365I+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+N474L、 H1 * +G7A+G73L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+K93R+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390D+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+V103I+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+E390A+N474L、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+R414M+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+Q360Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+S243Q+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+L205Y+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N422V+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474F、 H1 * +N3G+G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+N470M+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420P+G476H、 H1 * +G7A+R37A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N470M+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+H420P+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+H420A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+H323W+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+R414M+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G476A、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+H420A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G361M+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3G+G7A+V104M+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+N174K+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 N3G+G7A+R37V+D118T+W140M+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321Y+L354V+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470A+G475K、 H1 * +G7A+A31S+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1* + G7A + D118T + W140Y + R180* + S181* + T182D + E189P + N194F + L205Y + T224Y + Y242F + F266Y + N279S + K280Y + G303R + E390A + G476A, and 1. A method of producing an α-amylase variant from a parent α-amylase having the amino acid sequence of SEQ ID NO: 1 by introducing modifications selected from the group consisting of: H1* + G7A + D118T + W140Y + R180* + S181* + T182S + A185Q + E189P + N194F + Y242F + E259G + N279S + K280Y + S302T + E390A + G475K, wherein the α-amylase variant has at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to SEQ ID NO: 1; and (ii) mixing the α-amylase variant with a cleaning adjunct.

8. A method for improving the cleaning performance of a cleaning composition, comprising incorporating into the cleaning composition an α-amylase variant of a parent α-amylase having the amino acid sequence of SEQ ID NO: 1, wherein the α-amylase variant has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1, the method comprising the steps of: (a) introducing substitutions and / or deletions at two, three, or four positions in the parent α-amylase, the positions corresponding to positions 180, 181, 182, and 183, using SEQ ID NO: 1 for numbering; and (b) introducing substitutions and / or deletions at positions G7A, G7H, or G7S, and / or E390A or E390D, and at position H1, using SEQ ID NO: 1 for numbering. ＊ , H2 ＊ 、N3D、N3G、N3S、R26Y、A31S、R37A、R37N、R37V、T40G、T40N、A41D、N54S、V56T、A60V、A60P、L63F、K72R、G73L、K93R、N94D、Q98L、V103A、V103I、V103L、V104M、M105I、M105L、N106D、A109G、G110K、A111G、A111L、G113Q、G113S、G113A、T114K、F116L、F116N、F116Q、F116W、V117G、D118Q、D118T、D123N、N126D、N128Y、T131I、T131V、S132D、S132L、T134D、Y135E、Q136T、Q136K、I137F、W140S、W140M、W140Y、K142E、K142G、K142P、D144N、P146S、P146H、T151A、Y152L、F155W、W159H、W159L、Y160P、T165V、W167A、W167D、W167E、W167F、W167P、W167S、E169I、E169Q、E169V、K172G、K172Q、L173F、N174K、N174S、I176V、Y177D、K178A、A185D、A185E、A185K、A185N、A185Q、A185R、W188C、E189P、E193I、N194F、F202Y、F202Y、A203F、L205F、L205N、L205Q、L205R、L205V、L205Y、T224F、T224Y、N225S、Y242F、Y242L、S243Q、F244I、F244L、V252I、T256A、T256D、K258V、E259G、E259V、M260D、M260W、F261K、F261P、F261S、F266Y、N276F、N276W、N276Y、N279S、N280F、N280W、N280Y、W283N、S286K、S286L、S302T、S302Y、G303R、T315A、H320T、P321H、P321Y、M322G、M322Y、H323A、H323N、H323W、W346N、L354V、E359W、Q360M、Q360W、Q360Y、G361M、Y362V、V365I、Y371S、Y371N、A383D、N394W、N394D、F395Y、D405G、I409L、R414M、H420P、H420A、H420Q、introducing modifications at one or more positions corresponding to H420Y, N422V, T427L, G455R, K457N, K457R, R458S, N470A, N470M, S472R, V473C, N474F, N474L, G475E, G475K, G476H, G476E, and G476A; The α-amylase variants may be: H1 * +G7A+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180 * +T182L+G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+S181L+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180S+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+G113S+F116W+D123N+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+F116L+D123N+N126D+T131I+S181 * +T182 * +F202Y+Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+H320T+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261K+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+A383D+E390A+D405G+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +A60V+Q98L+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+A383D+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+N394W+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474L、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302T+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G475E、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474F、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+T256D+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242L+E259G+N279S+G303R+W346N+E390A+G475K、 H1 * +G7A+R37A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360Y+E390A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+R414M+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+F261P+N279S+G303R+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+H323W+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+P321Y+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360M+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+H420A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+V365I+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+N474L、 H1 * +G7A+G73L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+K93R+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390D+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+V103I+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+E390A+N474L、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+R414M+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+Q360Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+S243Q+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+L205Y+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N422V+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474F、 H1 * +N3G+G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+N470M+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420P+G476H、 H1 * +G7A+R37A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N470M+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+H420P+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+H420A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+H323W+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+R414M+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G476A、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+H420A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G361M+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3G+G7A+V104M+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+N174K+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 N3G+G7A+R37V+D118T+W140M+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321Y+L354V+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K, H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K, H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K, H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470A+G475K, H1 * +G7A+A31S+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K, H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A, H1* + G7A + D118T + W140Y + R180* + S181* + T182D + E189P + N194F + L205Y + T224Y + Y242F + F266Y + N279S + K280Y + G303R + E390A + G476A, and H1* + G7A + D118T + W140Y + R180* + S181* + T182S + A185Q + E189P + N194F + Y242F + E259G + N279S + K280Y + S302T + E390A + G475K, the α-amylase variant has at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% but less than 100% sequence identity to SEQ ID NO:1, and the α-amylase variant has α-amylase activity and improved cleaning performance compared to the parent α-amylase.

9. Use of a cleaning composition according to any one of claims 1 to 6 in a cleaning method.

10. The use according to claim 9, wherein the cleaning method is a laundry method or a hard surface cleaning method.

11. 1. A method for treating a surface, comprising: (i) water and an α-amylase variant of a parent α-amylase having the amino acid sequence of SEQ ID NO: 1, wherein the α-amylase variant is: H1 * +G7A+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180 * +T182L+G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+S181L+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+R180S+T182 * +G183 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+G113S+F116W+D123N+S181 * +T182 * +Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7A+F116L+D123N+N126D+T131I+S181 * +T182 * +F202Y+Y242F+E259G+N279S+G303R+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K, H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+H320T+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261K+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+E390A+G475K、 H1 * +G7S+T40N+R180 * +S181 * +Y242F+E259G+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+F261S+N279S+G303R+Y362V+E390A+G475K、 H1 * +G7S+T40N+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+R180 * +S181 * +Y242F+E259G+N279S+G303R+A383D+E390A+D405G+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7H+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +A60V+Q98L+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+A383D+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7H+Q98L+G110K+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+T151A+R180 * +S181 * +N194F+L205Y+Y242F+K258V+E259V+N279S+G303R+E390A+G475K、 H1 * +G7S+R180 * +S181 * +Y242F+E259G+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+N394W+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474L、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302T+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+G475E、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+N474F、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+T256D+N279S+G303R+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +N194F+L205Y+Y242L+E259G+N279S+G303R+W346N+E390A+G475K、 H1 * +G7A+R37A+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360Y+E390A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+E390A+R414M+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+F261P+N279S+G303R+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+H323W+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+P321Y+E390A+G475K、 H1 * +W140Y+R180 * +S181 * +N194F+L205Y+Y242F+E259G+N279S+G303R+Q360M+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+H420A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+V365I+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+N474L、 H1 * +G7A+G73L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+K93R+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390D+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+V103I+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+E390A+N474L、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+R414M+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321H+Q360Y+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+E259G+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+S243Q+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+L205Y+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+T40G+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+N422V+G475K、 H1 * +G7A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N474F、 H1 * +N3G+G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+N470M+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+Y362V+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y362V+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+N474F、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G476H、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420P+G476H、 H1 * +G7A+R37A+Q98L+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+N470M+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+E390A+H420P+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+E390A+H420A+N474L、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420P+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+H323W+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+R414M+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G476A、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+H420A+G476H、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+H420A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +N3G+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +N3D+G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+G361M+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+N474L、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G476A、 H1 * +G7A+D118T+W140Y+P146H+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+N474L、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3G+G7A+V104M+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3G+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+Y371N+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Y135E+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+H420A+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 H1 * +G7A+R37N+D118T+W140Y+N174K+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394W+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+H420A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+T256D+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132D+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394W+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394D+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G476H、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+N394D+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+S132L+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+N394W+G475K、 H1 * +G7A+D118T+Q138V+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+T224F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+G303R+N394D+G475K、 N3G+G7A+R37V+D118T+W140M+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+H323W+N394D+G475K、 H1 * +G7A+R37A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+G303R+P321Y+L354V+N394D+G475K、 H1 * +G7A+D118T+W140Y+R180Q+S181 * +T182 * +A185Q+E189P+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K、 H1 * +N3D+G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302T+E390A+H420A+G475K、 H1 * +G7A+R37N+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470M+G475K、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+E189P+N194F+T224Y+Y242F+N279S+K280Y+G303R+E390A+G476A、 H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182D+A185Q+N194F+Y242F+E259G+N279S+K280Y+S302T+E390A+G475K、 H1 * +G7A+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302Y+E390A+G475K, H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+L205Y+Y242F+E259G+M260D+N279S+K280Y+S302T+E390A+G475K, H1 * +N3D+G7A+D118T+W140Y+R180 * +S181 * +T182D+E189P+N194F+L205Y+Y242F+E259G+N279S+K280Y+S302T+E390A+H420A+G475K, H1 * +G7A+W140Y+R180 * +S181 * +E189P+N194F+Y242F+E259G+M260D+N279S+K280Y+S302Y+E390A+N470A+G475K, H1 * +G7A+A31S+R37N+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+F266Y+N279S+K280Y+S302Y+E390A+G475K, H1 * +G7A+D118T+W140Y+R180 * +S181 * +T182S+A185Q+E189P+N194F+Y242F+E259G+M260W+N279S+K280Y+G303R+E390A+G476A, H1* + G7A + D118T + W140Y + R180* + S181* + T182D + E189P + N194F + L205Y + T224Y + Y242F + F266Y + N279S + K280Y + G303R + E390A + G476A, and H1* + G7A + D118T + W140Y + R180* + S181* + T182S + A185Q + E189P + N194F + Y242F + E259G + N279S + K280Y + S302T + E390A + G475K, forming an aqueous wash solution comprising an α-amylase variant of a parent α-amylase, wherein the α-amylase variant has at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to SEQ ID NO: 1, and wherein the α-amylase variant has improved wash performance as measured by specific activity of an improvement factor (IF) > 1.0 when compared to the parent α-amylase; and a wash adjunct; (ii) treating the surface with the aqueous cleaning solution; (iii) rinsing the surface.

12. The method of claim 11, wherein the method for treating the surface is a method for treating fabric.

13. The method described in claim 11 or 12, wherein the α-amylase variant has improved cleaning performance relative to a measured specific activity of an improvement factor (IF) > 1.0 when compared to the α-amylase of sequence number 2 or 3.

Citation Information

Patent Citations

  • Amylase variants

    WO1996023873A1

  • Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same

    WO2000060060A2

  • Alpha-amylase variants and polynucleotides encoding same

    WO2015189371A1

  • Alpha-amylase variants

    WO2018141707A2

  • Cleaning compositions comprising amylase variants

    WO2018144399A1