Apparatus and observation position adjustment method

Abstract

To provide an instrument and a method for adjusting an observation position that allow a proper observation of diseases or insect damages that can happen to plants.SOLUTION: The present invention relates to an observation position adjusting instrument 10 used to observe a leaf 2 by a portable microscope 1, the instrument including: a light source 11 arranged on one side surface of a leaf 2, the light source being capable of irradiating the leaf 2 with light; and a light shielding plate 12 provided in such a position that the light shielding plate can block the light from the light source 11 in the direction of application of the light. The light shielding plate 12 has a light transmitting unit 15 for irradiating a predetermined irradiation range of one side surface of the leaf 2 with light, without blocking the light emitted from the light source 11.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an observation position adjustment device used when observing leaves with a portable microscope, and an observation position adjustment method for observing leaves using the observation position adjustment device. [Background technology]

[0002] Traditionally, plant damage caused by diseases and insect pests accounts for approximately 25% of production. To reduce damage to plants caused by such diseases and insect pests, it is necessary to use pesticides appropriately. In other words, to select the appropriate pesticide to use, it is necessary to accurately observe and identify the diseases and insect pests.

[0003] For example, Patent Document 1 discloses a method for identifying the disease that caused the abnormality by taking a photograph of an abnormal vegetable using a camera and comparing the obtained image data with a database of disease images stored in advance. This method identifies the disease using color tone and one or more characteristics of the disease. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2013-111078 Summary of the Invention [Problem to be solved by the invention]

[0005] However, when using a camera to distinguish between plants, the color tone of images taken is prone to change due to disturbances in the shooting environment or the process of plant growth, and the results of the distinction are not always accurate.

[0006] One way to observe the specimens while reducing disturbances and color changes is to use a portable microscope with a magnification of about 200x. However, this microscope has a field of view of 3mm. 2 The degree of mold is so narrow that it is difficult to see tiny or thin mold in the field of view.

[0007] An object of the present invention is to provide an observation position adjusting device and an observation position adjusting method that enable appropriate observation of diseases and insect damage that occurs in plants. [Means for solving the problem]

[0008] In order to achieve the above object, the present invention Device The characteristic configuration of the portable microscope and the microscope. An observation position adjustment device used when observing leaves by An apparatus comprising: And, The microscope includes a lens barrel, an eyepiece provided on one side of the lens barrel, and an objective lens provided on the other side of the lens barrel, and the observation position adjustment device includes: a light source that is arranged on one side of the leaf and that can irradiate light toward the leaf; and a light-shielding plate that is provided at a position that blocks light from the light source in the light-irradiation direction, wherein the light-shielding plate is provided with a light-transmitting portion that can irradiate a predetermined irradiation range on one side of the leaf without blocking the light irradiated from the light source; the light-transmitting portion is configured by an opening formed in the light-shielding plate, The light source is provided with a light-shielding portion at a position corresponding to the center of the light-transmitting portion, the light-shielding portion blocking light irradiated onto a predetermined irradiation range on one side of the leaf. By doing so, the light irradiated to the predetermined irradiation range on one side of the leaf becomes annular, and the light blocking portion is smaller than the size of the field of view of the microscope. It's at the point.

[0009] According to the above-described configuration, light emitted from the light source of the observation position adjustment device is irradiated onto one side of the leaf through the light-transmitting portion provided in the light-shielding plate. This light passes through the leaf and can be seen from the other side, which is the underside of the one side of the leaf. If the observation target portion, which is the portion of the leaf to be observed with the microscope, is positioned so that it is included in a predetermined illumination range, i.e., the illumination range of the light transmitted through the other side of the leaf, the observation target portion of the leaf can be easily brought into view by simply positioning the microscope so that the illumination range of the light transmitted through the other side of the leaf fits within the field of view. This makes it easy to observe the observation target portion. Furthermore, because the light emitted from the light source is blocked by the shading portion, an annular light is irradiated onto one side of the leaf and annular light is transmitted through the other side of the leaf. The observation position adjustment device is positioned so that the leaf to be observed is located at the center of the annular light, that is, the portion of the light transmitted through the other side of the leaf that is blocked by the shading portion, and the microscope is positioned so that the portion blocked by the shading portion is within the field of view of the microscope; this allows the observation of the leaf to be easily brought into view.

[0010] Because the portable microscope is used to observe abnormalities in leaves, it is possible to check for abnormalities in leaves from a closer distance than when using a conventional camera to photograph abnormalities in leaves. Because the leaves can be observed from a closer distance than with a camera, the influence of disturbances in the observation environment can be reduced. This can improve the accuracy of identifying diseases and pests.

[0013] The present invention Device A further characteristic feature of the present invention is that the light source is an LED light.

[0014] Since the light source is an LED light, it generates little heat, and there is no risk of the heat from the light source affecting the leaves when observing them.

[0015] The present invention Device A further characteristic feature of the present invention is that the light source and the light blocking plate are mounted in a common housing.

[0016] The light source and the light transmitting portion are installed in a common housing, which makes it easy to handle.

[0017] In order to achieve the above object, the observation position adjustment method according to the present invention has the following characteristic configuration: ,above A device having any of the above features and configurations Device An observation position adjustment method for observing leaves using The aforementioned The method includes an irradiation position adjustment step of moving the observation position adjustment device relative to the leaf so that light emitted from a light source is irradiated onto a predetermined irradiation range on one side of the leaf, and an observation position adjustment step of positioning the microscope so that the irradiation range of light transmitted to the other side of the leaf falls within the field of view of the microscope.

[0018] According to the above-described configuration, light emitted from the light source of the observation position adjustment device is irradiated onto one side of the leaf through the light-transmitting portion provided in the light-shielding plate. This light passes through the leaf and can be seen from the other side, which is the underside of the one side of the leaf. If the observation target portion, which is the portion of the leaf to be observed with the microscope, is positioned so that it is included in a predetermined illumination range, i.e., the illumination range of the light transmitted through the other side of the leaf, the observation target portion of the leaf can be easily brought into view by simply positioning the microscope so that the illumination range of the light transmitted through the other side of the leaf fits within the field of view. This makes it easy to observe the observation target portion.

[0019] A further characteristic feature of the observation position adjusting method according to the present invention is that the microscope , Teru The microscope has a bright portion and a cylindrical guide arranged to surround the field of view of the microscope, and the observation position adjustment process is performed by relatively aligning the irradiation range of the light transmitted to the other side of the leaf with the cylindrical guide.

[0020] The observation position adjustment process can be performed by the simple task of abutting the cylindrical guide against the leaf so that the relative position of the cylindrical guide matches the irradiation range of the light transmitted to the other side of the leaf, and therefore does not require skill in the observation work. [Brief explanation of the drawings]

[0021] [Figure 1] FIG. 10 is a schematic diagram of an observation position adjustment device. [Figure 2] FIG. 10 is an explanatory diagram of when light is irradiated onto a leaf using an observation position adjustment device. DETAILED DESCRIPTION OF THE INVENTION

[0022] The observation position adjusting device and the observation position adjusting method according to the present invention will be described below.

[0023] FIG. 1 shows a portable microscope 1, a leaf 2 that is an object to be observed by the microscope 1, and an observation position adjustment device 10 according to the present invention.

[0024] In this embodiment, the microscope 1 is an optical microscope. The microscope 1 includes a lens barrel 4, an eyepiece 5 provided on one side of the lens barrel, an objective lens 6 provided on the other side of the lens barrel 4, an LED light (not shown) as an illumination unit that illuminates the field of view, and a cylindrical guide 7 provided to surround the field of view of the microscope. The cylindrical guide 7 is a cylindrical body made of resin such as transparent plastic, and is configured so that by abutting the cylindrical guide 7 against the surface of the leaf 2, the distance between the objective lens 6 and the leaf 2 falls within the adjustable range of the focus of the microscope 1.

[0025] The leaf 2 has an observation target portion 3. The observation target portion 3 is a portion of the leaf 2 where an abnormality has occurred due to disease or pests.

[0026] The observation position adjustment device 10 includes a light source 11, a light shielding plate 12, and a housing 13 in which the light source 11 and the light shielding plate 12 are installed.

[0027] The light source 11 is composed of an LED light and is configured to be able to irradiate light toward the leaf 2. However, the light source 11 is provided with a light-shielding portion 14 at a position corresponding to the center of a light-transmitting portion 15 (described later) that blocks light irradiated to a predetermined irradiation range on one side of the leaf 2.

[0028] The shape and dimensions of light-shielding portion 14 are determined according to the size of the field of view of microscope 1 used. For example, when the field of view of microscope 1 is about Φ2 mm, light-shielding portion 14 has a diameter of about Φ1 mm. The shape of light-transmitting portion 15 is not limited to a circle and may be, for example, a rectangle.

[0029] The light blocking plate 12 is provided at a position in the light irradiation direction where it blocks light from the light source 11, and is configured to block light from the light source 11. The light blocking plate 12 is made of a resin such as black polyvinyl chloride. In FIG. 1, the light blocking plate 12 is provided at a position separated from the light source 11. However, the light blocking plate 12 may be provided at a position where it contacts the surface of the light source 11.

[0030] The light-shielding plate 12 is provided with a light-transmitting portion 15 that can irradiate a predetermined irradiation range on one side of the leaf 2 without blocking the light emitted from the light source 11. The shape and dimensions of the light-transmitting portion 15 are determined according to the shape and dimensions of the objective lens 6 of the microscope 1 used. For example, when the diameter of the cylindrical guide 7 of the microscope 1 is Φ25 mm, the light-transmitting portion 15 has a diameter of approximately Φ25 mm. The light-transmitting portion 15 can be composed of an opening formed in the light-shielding plate 12. A light-transmitting window may be disposed in the opening.

[0031] The housing 13 is formed by processing a plate-shaped body made of resin such as acrylic. In this embodiment, the housing 13 has dimensions of approximately 5 cm x 5 cm x 5 cm. Note that these dimensions are merely an example, and the housing 13 may be provided with a grip for the operator to hold.

[0032] With the above configuration, the light emitted from the light source 11 is blocked by the light blocking plate 12 and the light blocking portion 14, so that the light irradiated onto the leaf 2 is annular.

[0033] The following describes a method for adjusting an observation position using the microscope 1 and the observation position adjusting device 10 configured as described above.

[0034] The observation position adjusting method includes an irradiation position adjusting step and an observation position adjusting step.

[0035] In the irradiation position adjustment step, the observation position adjustment device 10 is moved relative to the leaf 2 so that the light emitted from the light source 11 is irradiated onto a predetermined irradiation range on one side of the leaf 2. As a result, the light passes through the leaf 2 and can be seen from the other side, which is the reverse side of the one side of the leaf 2. As shown in FIG. 2, if the observation target portion 3 of the leaf 2 is positioned so that it is included in the predetermined irradiation range, i.e., the irradiation range of the light transmitted to the other side of the leaf 2, the observation target portion 3 of the leaf 2 can be easily brought into view by simply positioning the microscope 1 so that the irradiation range of the light transmitted to the other side of the leaf 2 fits within the field of view. This makes it easy to observe the observation target portion.

[0036] When placing the microscope 1 on the other side of the leaf 2, the illumination range of the light transmitted through the other side of the leaf 2 is aligned relatively with the cylindrical guide 7 of the microscope 1, and then the cylindrical guide 7 is brought into contact with the leaf 2. The observation position adjustment step can be performed by the simple task of aligning the illumination range of the light transmitted through the other side of the leaf 2 relatively with the cylindrical guide 7, and therefore does not require skill in the observation work.

[0037] In the above-described embodiment, the observation position adjustment device 10 is provided with the light source 11 provided with the light blocking portion 14, but this is not limited thereto. The light source 11 does not necessarily have to be provided with the light blocking portion 14.

[0038] In the above-described embodiment, the observation position adjustment device 10 has been described as including a housing 13 in which the light source 11 and the light shielding plate 12 are installed, but this is not limited thereto. For example, the observation position adjustment device 10 may not include a housing 13, and the light source 11 of the observation position adjustment device 10 may include an LED and a circuit board in the internal space of a metal frame, a diffuser plate that can transmit light from the LED in a diffused state and is attached to the frame, and the light shielding plate 12 and the light shielding portion 14 are further attached directly to the diffuser plate. Such a light source 11 may have dimensions of, for example, approximately 20 cm x 16 cm x 5 cm in length, width, and height. Note that these dimensions are merely examples.

[0039] In the above-described embodiment, the microscope 1 is an optical microscope, but this is not limited to this. For example, the microscope 1 may be a digital microscope that has a photographing function and can transmit photographed images to a smartphone or a personal computer.

[0040] The image data captured by such a microscope 1 is subjected to disease and pest discrimination based on an image analysis database stored in advance on a smartphone or personal computer. For such discrimination, a model using transfer learning of VGG16, for example, is used.

[0041] The configurations disclosed in the above embodiments (including other embodiments, the same applies below) can be applied in combination with configurations disclosed in other embodiments, as long as no contradiction arises. Furthermore, the embodiments disclosed in this specification are examples, and the embodiments of the present invention are not limited to these, and can be modified as appropriate within the scope that does not deviate from the purpose of the present invention. [Explanation of symbols]

[0042] 1: Microscope 2:Leaf 3: Observation target area 4: Telescope tube 5: Eyepiece 6: Objective lens 7: Cylindrical guide 10: Observation position adjustment device 11:Light source 12: Shade 13: Housing 14: Light blocking section 15:Transparent part

Claims

1. An apparatus comprising a portable microscope and an observation position adjustment device used when observing leaves with the microscope, the microscope comprises a lens barrel, an eyepiece provided on one side of the lens barrel, and an objective lens provided on the other side of the lens barrel; The observation position adjustment device is a light source disposed on one side of the leaf and capable of irradiating light toward the leaf; a light blocking plate provided at a position to block light from the light source in the light irradiation direction, The light blocking plate is provided with a light-transmitting portion that can irradiate a predetermined irradiation range on one side of the leaf without blocking the light irradiated from the light source, the light-transmitting portion is configured by an opening formed in the light-shielding plate, a light-shielding portion that blocks light irradiated onto a predetermined irradiation range on one side surface of the leaf at a position corresponding to the center of the light-transmitting portion in the light source, so that the light irradiated onto the predetermined irradiation range on one side surface of the leaf becomes annular; The light-blocking portion is smaller than the size of the field of view of the microscope.

2. The device of claim 1 , wherein the light source is an LED light.

3. 3. The device of claim 1, wherein the light source and the gobo are mounted in a common housing.

4. A method for adjusting an observation position for observing leaves using the device according to any one of claims 1 to 3, comprising: an irradiation position adjusting step of moving the observation position adjusting device relative to the leaf so that the light emitted from the light source is irradiated onto a predetermined irradiation range on one side of the leaf; and an observation position adjusting step of positioning the microscope so that the illumination range of the light transmitted through the other side of the leaf falls within the field of view of the microscope.

5. The microscope includes an illumination unit and a cylindrical guide provided to surround a field of view of the microscope, 5. The observation position adjusting method according to claim 4, wherein the observation position adjusting step is performed by relatively aligning the irradiation range of the light transmitted through the other side surface of the leaf with the cylindrical guide.

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