cleaning agents

The cleaning agent combines proteolytic enzymes, bamboo charcoal, and collagen to address the challenge of providing effective cleansing and skin-conditioning effects, ensuring stability and performance over time.

JP7790714B2Active Publication Date: 2025-12-23TSUTSUMI PLANNING CO LTD
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Patent Information

Application Number
JP2022040515
Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
Filing Date
2022-03-15
Publication Date
2025-12-23
Estimated Expiration
2042-03-15

AI Technical Summary

Technical Problem

Existing detergents struggle to provide both high levels of cleansing and skin-conditioning effects, and their effectiveness can be reduced by the inclusion of skin-conditioning ingredients or inactivated by external factors, leading to instability over time.

Method used

A cleaning agent comprising homogenized bacteria or yeast with protein digestive enzymes, bamboo charcoal powder, and collagen powder, which includes proteolytic components like fungal extract, papain, and pancreatin, to enhance cleansing and skin-conditioning effects while maintaining stability over time.

Benefits of technology

The cleaning agent achieves high levels of cleansing and skin-conditioning effects, with improved foaming and foam persistence, and maintains these properties even after long storage periods.

✦ Generated by Eureka AI based on patent content.

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Abstract

To provide a cleaning agent that has both cleaning and skin-conditioning effects at a high level and also provide a cleaning agent that maintains its sufficient cleansing and skin-conditioning effects even after a long storage period.SOLUTION: A cleaning agent comprises a proteolytic enzyme agent, vegetable charcoal powder, and collagen powder.SELECTED DRAWING: None
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Description

[Technical Field]

[0001] The present invention relates to a cleaning agent. [Background technology]

[0002] Conventionally, detergents have been used to efficiently remove dirt from objects to be cleaned. Specifically, detergents are proposed according to the object to be cleaned, such as laundry detergent for muddy stains on clothes, dishwashing detergent for grease and protein stains on dishes, and facial soap for sebum and keratin stains on the face, and each detergent is used according to its purpose.

[0003] The detergent contains surfactants, which have a cleaning effect on grease and protein stains that are difficult to remove with water alone.

[0004] However, surfactants alone may not be able to provide sufficient cleaning power for protein stains, and so detergents containing proteolytic enzymes have been proposed to more reliably remove protein stains (e.g., Patent Document 1). [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Japanese Patent Application Laid-Open No. 2013-103951 Summary of the Invention [Problem to be solved by the invention]

[0006] It is true that the above-mentioned detergents can be expected to more reliably remove proteinaceous stains by fully utilizing the cleaning action contributed by protease. However, such detergents have the following problems.

[0007] For example, when a cleanser is used directly on the scalp, it may remove excess keratin and sebum from the scalp along with dirt, causing rough skin. To prevent such rough skin, some cleansers contain sebum components, polyhydric alcohols, carbohydrates, and other ingredients that have a skin-conditioning effect. However, the inclusion of skin-conditioning ingredients reduces the cleansing effect, and as a result, no cleanser can be produced that provides both high levels of cleansing and skin-conditioning effects.

[0008] Furthermore, while enzymes are useful for removing proteinaceous stains, they can be inactivated by external factors (such as high temperatures or exposure to light), and there is a need for a cleanser that can maintain its cleansing and skin-conditioning effects even after a longer storage period.

[0009] The present invention has been made in view of the above circumstances, and aims to provide a cleanser that has both high levels of cleansing and skin-conditioning effects, and that maintains its cleansing and skin-conditioning effects even after a long storage period. [Means for solving the problem]

[0010] In order to solve the above-mentioned problems of the prior art, the cleaning agent according to the present invention comprises: (1) It consists of homogenized bacteria or yeast containing protein digestive enzymes. A proteolytic enzyme agent, 0.01 to 20% by weight of bamboo that passes through a 50 to 180 mesh sieve carbide powder, 0.01 to 20.5% by weight of porcine-derived fat passing through a 20 to 200 mesh sieve Blended with collagen powder Pass through a 20 to 1000 mesh sieve A cleaning agent comprising: The collagen powder has a total content of proline and hydroxyproline of 15 to 25% by weight. The present invention is characterized by the above.

[0011] The cleaning agent according to the present invention is also characterized in the following respects. (2) The proteolytic enzyme preparation contains a proteolytic component having peptidase activity. (3) The proteolytic enzyme preparation contains one or more of fungal extract, papain, and pancreatin as a proteolytic component. [Effects of the Invention]

[0015] Book According to the invention, by blending a proteolytic enzyme agent, a carbonized plant powder, and a collagen powder, it is possible to provide a cleanser that exerts both cleansing and skin-conditioning effects at a high level, and that maintains its cleansing and skin-conditioning effects sufficiently even after a long storage period. Furthermore, because the plant carbonized powder is contained in an amount of 0.01 to 20% by weight, a sufficient amount of the plant carbonized powder is evenly distributed throughout the bubbles, allowing the plant carbonized powder to fully adsorb dirt. As a result, a cleaning agent with a more reliable cleaning effect can be provided. Furthermore, the carbonized powder acts as a binder for the foam, allowing for the creation of fine, sustained foam.

[0016] Also The protease agent contains a proteolytic component with peptidase activity, providing a cleanser that can more reliably remove protein stains. Furthermore, the proteolytic component acts on the collagen powder to generate amino acids, which then act on bubbles, enhancing foaming and foam persistence. This allows highly elastic bubbles composed of fine bubbles to remain for a long period of time. As a result, a cleanser that can more reliably enjoy both cleansing and skin-conditioning effects can be provided.

[0017] The protease preparation contains a fungal extract as a proteolytic component. Things The inclusion of these components makes it possible to provide a cleanser that can more reliably remove proteinaceous stains. Furthermore, these proteolytic components act on the collagen powder to produce amino acids, which then act on bubbles, enhancing foaming and foam persistence. This allows highly elastic bubbles composed of fine bubbles to remain for a long period of time. As a result, it is possible to provide a cleanser that can more reliably enjoy both cleansing and skin-conditioning effects.

[0018] The plant carbonized powder is Bamboo Because it is a carbide powder, the numerous pores formed on the surface can adsorb a large amount of dirt, resulting in the provision of a cleaning agent that can more reliably enjoy cleaning action.

[0019] Also The plant carbonized powder passes through a 50 to 180 mesh sieve, which has the effect of providing a cleaning agent that can more reliably enjoy cleaning action while minimizing the foreign body sensation when it comes into contact with air bubbles.

[0021] Also , the collagen powder , Pig origin Since the raw materials are naturally available, it is relatively easy to procure them, which has the effect of providing a cleaning agent that is advantageous in terms of production costs.

[0022] Also The collagen powder passes through the gaps of a 20-200 mesh sieve, and therefore quickly reacts with protease to produce amino acids, enhancing foaming and foam persistence. It also minimizes the foreign body sensation felt when coming into contact with air bubbles. As a result, it is possible to provide a cleanser that can more reliably enjoy both cleansing and skin-conditioning effects.

[0023] Also The collagen powder is contained in an amount of 0.01 to 20.5 parts by weight per 100 parts by weight of the cleanser, thereby enhancing foaming and foam retention. In particular, highly elastic foam consisting of fine bubbles can be retained for a long period of time. Furthermore, the collagen powder that did not react with the protease and some of the reaction products serve to coat and protect the skin. As a result, a cleanser that can more reliably enjoy both cleansing and skin-conditioning effects can be provided.

[0024] Also The total content of proline and hydroxyproline in the collagen powder is set to 15 to 25% by weight, which improves foaming ability and foam persistence, resulting in the provision of a cleanser that can more reliably provide cleansing and skin-conditioning effects.

[0025] Also The above-mentioned cleanser passes through a 20 to 1000 mesh sieve, which allows for a rapid reaction between the protease and collagen powder, and also enhances foaming and foam persistence, resulting in the provision of a cleanser that can more reliably provide both cleansing and skin-conditioning effects. [Brief explanation of the drawings]

[0026] [Figure 1] 1 is a line graph showing the results of Test 1. [Figure 2] 1 is a bar graph showing the results of Test 2. [Figure 3] 1 is a bar graph and table showing the results of Test 3. [Figure 4] 1 is a bar graph and table showing the results of Test 4. DETAILED DESCRIPTION OF THE INVENTION

[0027] The gist of the present invention is to provide a cleanser that combines a protease agent, a carbonized plant powder, and a collagen powder, thereby exhibiting high levels of cleansing and skin-conditioning effects, and that can maintain these effects even after a long storage period.

[0028] Here, the detergent according to the present invention is a detergent containing a surfactant and used for the purpose of removing dirt, regardless of whether it is for home or commercial use. Specific examples include laundry detergents, dishwashing detergents, cleaning detergents, facial cleansers, and hair washes (shampoos). In this embodiment, a facial cleanser will be used as an example.

[0029] The facial cleanser according to this embodiment is in a granular or powder form in a dry state (water content approximately 14%). Specifically, the form can be freely selected as long as it can pass through a sieve with a mesh size of 20 (opening 864 μm) to 1000 (opening 13 μm).

[0030] The facial cleanser according to this embodiment is used for washing by, for example, taking a predetermined amount of the cleanser in the palm of the hand, adding water or hot water to create a lather. In particular, by making the shape of the cleanser fall within the above range, the contact area with water can be increased, allowing the surfactant to lather quickly and allowing the protease agent and collagen powder agent described below to react quickly. In the following description, the water or hot water used to dissolve the facial cleanser and lather it will be referred to as "dissolution water," and the state in which the cleanser is dissolved in the dissolution water will be referred to as "facial cleanser."

[0031] In addition, if the shape of the facial cleanser contains many substances that do not pass through a 20 mesh sieve, it will dissolve slowly in the dissolution water, which will reduce the feeling of use. However, even if the shape does not pass through the above mesh sieve, there is no problem as long as it can be crushed by pinching or rubbing with fingers. On the other hand, if the shape contains many substances that pass through a sieve with openings narrower than 1000 mesh, the feeling of use will not be reduced, but it is not preferable because it may take too long to complete the manufacturing process or there may be many non-standard shapes.

[0032] The facial cleanser contains a surfactant, an excipient, a protease agent, a plant carbonized powder, and a collagen powder, each of which is in a dry state. In particular, by mixing these in a dry state, the hydrolysis reaction of the protease agent with the collagen powder can be suppressed. In other words, the facial cleanser maintains the collagen powder in its original state until it comes into contact with dissolving water, but when it is made into a facial cleanser, it starts to react with the protease agent. Next, each component of the facial cleanser will be described in detail.

[0033] The surfactant can be freely selected as long as it functions as a cleaning component. Specifically, it can be selected from anionic surfactants, amphoteric surfactants, nonionic surfactants, fatty acid soaps, etc.

[0034] The excipient is used to improve the shape and feel of the detergent, and can be any of the excipients commonly used in existing detergents, such as cornstarch, lactose, crystalline cellulose, and starch.

[0035] The protease agent may be any agent capable of decomposing proteins present on the skin surface, such as aged, unnecessary keratin and dirt, and may contain, for example, a proteolytic component having peptidase activity. More preferably, one or more of fungal extract, papain, and pancreatin may be used.

[0036] The fungal extract may contain protein-digesting enzymes possessed by fungi. Specifically, fungal cells are collected, crushed, and dried. In addition to the proteolytic components, the fungal extract also contains components obtained by culturing fungi, such as amino acids and sugars produced by autolysis. Thus, the proteolytic enzyme preparation may contain amino acids, sugars, and the like, other than the proteolytic components. Fungi include eubacteria, yeast, and the like.

[0037] Papain is known to be found in large amounts in the fruits and juice of unripe papaya plants, and therefore, dried papain from the fruits and juice of papaya plants or a dried extract is used.

[0038] Pancreatin is a mixture of various physiologically active components obtained from the mammalian pancreas, including trypsin, chymotrypsin, lipase, amylase, and many other enzymes, and is known as a mixture of digestive enzymes that are effective against starch, fat, and protein. Thus, proteolytic enzyme preparations may contain enzymes other than proteolytic components.

[0039] The protease agent may be a combination of two or more of these proteolytic components. The optimum pH of the protease agent is not limited to acidic, neutral, or alkaline, but the facial cleanser may have an optimum pH ranging from weakly acidic to alkaline, or from neutral to alkaline.

[0040] The total activity titer of the proteolytic components contained in the protease preparation is characterized by being 0.1 U to 300 U / g in the detergent when measured by a quantitative test method conforming to the pancreatin quantitative regulations of the Japanese Pharmacopoeia.

[0041] By using such a protease, protein stains can be removed more reliably when the facial cleanser is lathered and used for face washing. Furthermore, the protease acts on the collagen powder in the facial cleanser, cleaving the terminal amino acids of the collagen-forming polypeptide chains. Therefore, the collagen-derived peptide chains and amino acids function as thickeners, allowing the bubbles to become meringue-like.

[0042] The plant charcoal powder is a pulverized product of at least one char selected from bamboo, wood, and nut seeds, and is particularly suitable for use in plants that pass through a 50-mesh (279 μm mesh) to 180-mesh (84 μm mesh) sieve. This plant charcoal powder has multiple finely branched pores formed on its surface (a so-called porous structure). This porous structure allows the plant charcoal powder to effectively adsorb protein and sebum stains. Depending on the size of the pores, they can be classified as micropores (diameter 20 Å or less), mesopores (diameter 20 to 500 Å), or macropores (diameter 500 Å or more). Micropores play a major role in the actual adsorption of protein and sebum stains. This adsorption is primarily physical adsorption due to van der Waals forces or capillary action.

[0043] Meanwhile, the mesopores and macropores have the effect of absorbing the meringue-like bubbles formed by the proteolytic enzyme and collagen powder. As a result, when using the facial cleanser (when lathering), the carbonized powder particles rub against each other, crushing the absorbed bubbles, further minimizing the bubbles. The mesopores and macropores also function as binders that connect the bubbles together. This minimizes the surface area of ​​the bubbles that come into contact with the air, thereby improving foam retention.

[0044] In this way, by adding plant carbonized powder to a facial cleanser containing a protease agent and collagen powder, it is possible to not only adsorb protein and sebum stains, but also further improve foaming properties and foam persistence.

[0045] If the shape of the plant carbonized powder contains a large amount of particles that do not pass through a 50-mesh sieve, the facial cleanser will have a poor feel when foamed, and the adsorption effect will not be satisfactory due to the reduced surface area. On the other hand, if the powder contains a large amount of particles that pass through a sieve with openings narrower than 180 mesh, the feel will not be reduced, but the manufacturing process may take too long or there may be many irregular shapes, which is not preferable.

[0046] Therefore, the plant carbonized powder is expected to enhance the cleansing effect of the facial cleanser by adsorbing and removing sebum and proteinaceous dirt decomposed by protease. In particular, if the plant carbonized powder is contained in an amount of 0.01 to 20 wt%, a facial cleanser that can reliably adsorb and remove sebum and proteinaceous dirt can be provided.

[0047] If the plant charcoal powder is contained in an amount of less than 0.01% by weight, a sufficient adsorption effect cannot be obtained, and on the other hand, if the amount is more than 20% by weight, the adsorption effect does not increase in proportion to the content, so it is not preferable from the viewpoint of cost.

[0048] The collagen powder is characterized by containing collagen derived from, for example, fish, birds, pigs, cattle, and horses. More specifically, the collagen is obtained by spray-drying or vacuum freeze-drying extracts such as atelocollagen (molecular weight: approximately 300,000) obtained by treating the biological tissues of the above non-human animals with enzymes or alkalis, gelatin (molecular weight: approximately 100,000) obtained by subjecting atelocollagen to heat treatment and hydrolysis, and collagen peptide (molecular weight: approximately 1500) obtained by subjecting gelatin to acid, base, or enzyme treatment. Furthermore, excipients and preservatives such as vitamins can be added as needed. The dried collagen is then crushed into a shape that can pass through a 20-mesh (864 μm mesh) to 200-mesh (74 μm mesh) sieve to produce the collagen powder. This shape facilitates reaction with proteolytic enzymes.

[0049] If the shape of the collagen powder contains a large amount of particles that do not pass through a 20-mesh sieve, the surface area will decrease, hindering smooth reaction with the protease.On the other hand, if the shape of the collagen powder contains a large amount of particles that pass through a sieve with openings narrower than 200 mesh, although the collagen will react sufficiently with the protease, it is not suitable because the manufacturing process may take too long or there may be many particles with non-standard shapes.

[0050] When the collagen powder is formulated to contain 0.01 to 20.5% by weight of collagen, it can be reacted with the protease agent reliably, thereby improving foaming ability and foam retention. In addition, the collagen peptide chains function as skin conditioning ingredients.

[0051] If the collagen powder content is less than 0.01 wt%, the foaming ability and foam retention cannot be sufficiently improved, while if the collagen powder content is more than 20.5 wt%, the foaming ability and foam retention do not increase in proportion to the content, which is not preferable from the viewpoint of cost.

[0052] The collagen powder used contains 15 to 25% by weight of proline and hydroxyproline in total (15 to 25 parts by weight of proline and hydroxyproline per 100 parts by weight). Proline and hydroxyproline are known to be amino acids abundantly contained in type I collagen and type II collagen. Furthermore, proline and hydroxyproline are known to have particularly high water-retaining capacity among amino acids.

[0053] In other words, even if the facial cleanser contains less than 0.01 wt % or more than 20.5 wt % collagen, proline and hydroxyproline may be contained in an amount equivalent to the amount of hydroxyproline contained when the facial cleanser contains 0.01 to 20.5 parts by weight of collagen, that is, 0.0015 to 5.125 wt %.

[0054] When the proline and hydroxyproline are contained in such a range, the proline and hydroxyproline function as a thickener to make the entire foam into a meringue-like state, thereby improving foaming ability and foam persistence, and providing a cleanser that can more reliably enjoy both cleansing and skin-conditioning effects.

[0055] The cleaning agent according to this embodiment will be further described below with reference to various test results.

[0056] [Test 1. Stability test of proteolytic components] In this study, we investigated how the presence or absence of plant-derived charcoal powder and collagen powder affects the stability of protease preparations in facial cleansers. Specifically, we investigated the effects of surfactants and excipients, Sample A: Contains proteolytic enzymes. Sample B: A combination of proteolytic enzymes and collagen powder. Sample C: A combination of proteolytic enzymes, collagen powder, and plant charcoal powder. A confirmation test was conducted to examine the effect of the above on the proteolytic components in the three samples.

[0057] (Test 1-1. Sample preparation) In this example, coconut shell activated carbon (manufactured by UES) was used as the plant-based carbonized powder, protease (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) was used as the proteolytic enzyme, and powdered research-grade collagen (manufactured by Nippi Co., Ltd.) was used as the collagen powder. The same materials were used in subsequent tests.

[0058] The coconut shell activated carbon (hereinafter simply referred to as "activated carbon") used was one that passed through a 50-mesh sieve. The powdered collagen for research testing (hereinafter simply referred to as "collagen") used was one that passed through a 20-mesh sieve. The same materials were used in subsequent tests.

[0059] In addition, fatty acid soap was used as the surfactant and cornstarch was used as the excipient. The fatty acid soap and cornstarch were mixed together and made into granules that could pass through a 20-mesh sieve. The same granules were used in subsequent tests.

[0060] The amounts of ingredients blended into each of Samples A, B, and C are shown in Table 1. The values ​​in the table are in weight percent. [Table 1]

[0061] First, 3g of each sample was placed in a 90mm diameter, 20mm high petri dish (disposable dish, manufactured by AS ONE Corporation). The petri dish was then placed in an incubator set at 40°C and 75% humidity. The effects on each sample were then tested every month (from 0 to 6 months).

[0062] (Test 1-2. Test Method) The proteolytic component stability test was performed according to the quantitative test method for pancreatin specified in the Japanese Pharmacopoeia. That is, the enzyme activity value at 0 months was set to 100%, and the percentage change in the enzyme activity value over time was examined.

[0063] (Test 1-3 Results) The test results are shown in Figure 1. As can be seen from Figure 1, the enzyme activity of Sample A decreased over time, whereas that of Samples B and C did not. In particular, the enzyme activity of Sample C did not decrease over a longer period than that of Sample B.

[0064] These results show that the blending of collagen into a facial cleanser containing a protease agent can increase the stability of the proteolytic components. In particular, the blending of collagen and activated carbon can increase the stability of the proteolytic components for an even longer period of time. Therefore, it can be said that the blending of a protease agent, a plant carbonized powder, and a collagen powder can provide a cleanser that fully exerts its cleansing and skin-conditioning effects.

[0065] [Test 2. Foaming and foam persistence test] In this study, we investigated how the presence or absence of plant-derived carbonized powder, collagen powder, and proteolytic agent affects the foaming and foam retention of facial cleansers. Sample A: Contains proteolytic enzymes. Sample B: A combination of proteolytic enzymes and collagen powder. Sample C: A combination of proteolytic enzymes, collagen powder, and plant carbonized powder. Sample D: Contains collagen powder. Sample E: Contains plant carbonized powder. Sample F: A blend of plant carbonized powder and collagen powder. The foaming properties and foam persistence of the six samples were confirmed through a confirmation test.

[0066] (Test 2-1. Sample preparation) The amounts of ingredients blended into each of Samples A, B, C, D, E, and F are as shown in Table 2. The values ​​in the table are in weight percent. [Table 2]

[0067] First, 0.1 g of each sample was placed in a 200 mL beaker together with 100 mL of dissolution water at 40° C. Then, the sample was completely dissolved using a stirrer without creating bubbles.

[0068] (Test 2-2. Test Method) 50 mL of each sample was gently placed in a 100 mL measuring cylinder, capped, and shaken up and down 100 times. The foam height immediately after shaking was recorded as foaming, and the foam height after leaving the cylinder to stand for 10 minutes was recorded as foam persistence. The foam height was measured based on the boundary between the cleaning solution and the foam.

[0069] (Test 2-3. Results) The results of this test are shown in Figure 2. As shown in Figure 2, compared to the foaming ability and foam retention of Sample A, Samples B and C had increased foaming ability. Furthermore, comparing the results of Sample B and Sample D, Sample B showed higher foaming ability and foam retention. Furthermore, comparing the results of Sample C and Sample F, Sample C showed higher foaming ability and foam retention. Furthermore, comparing the results of Sample E and Sample F, Sample F showed higher foaming ability and foam retention.

[0070] These results indicate that facial cleansers containing protease agents can improve foaming and foam retention by incorporating collagen powder. In particular, the addition of plant carbonized powder and collagen powder can further improve foam retention.

[0071] It was also revealed that even if a facial cleanser contains collagen powder or plant carbonized powder, it will not be able to achieve high foaming properties and foam persistence unless it also contains a proteolytic enzyme agent.

[0072] Therefore, it can be said that a facial cleanser containing all three of the following ingredients has higher foaming properties and foam retention than a cleanser containing only one or two of the following ingredients: proteolytic enzyme agent, plant carbonized powder, and collagen powder.

[0073] [Test 3. Dirt removal function test] In this test, we investigated how the presence or absence of plant-derived carbonized powder, collagen powder, and proteolytic agent affects the ability of a facial cleanser to remove artificial dirt (hereinafter also referred to as "dirt removal function"). The test samples used were the same as those used in Test 2.

[0074] (Test 3-1. Sample preparation) In this test, the foam was prepared in the same way as in Test 2, and the foam immediately after shaking was used for the test. The simulated soil was a mixture of 30 g of egg white, 15 mL of salad oil, and red food coloring.

[0075] (Test 3-2. Test Method) Six test areas, each 2 cm square, were set up on the inside of the forearms of five subjects. 1 g of simulated soil was applied to each test area and allowed to dry naturally for 20 minutes. After air drying, each sample was applied to cover the simulated soil and left for 1 minute. After leaving it, 40°C water was poured over the outside of the forearm, and the extent to which the simulated soil was removed was evaluated on a 5-point scale. The criteria for judging the soil removal function are shown in Table 3. [Table 3]

[0076] (Test 3-3. Results) The results of this test are shown in Figure 3. Figure 3 shows that Samples B, C, and F have superior stain removal capabilities compared to Sample A. Sample C in particular is superior to the other samples.

[0077] These results suggest that the dirt-removing function of a protease-containing detergent can be enhanced by blending it with collagen powder. In particular, blending plant carbonized powder with collagen powder can further enhance the dirt-removing function.

[0078] It was also revealed that even if a powder of plant carbonized material or a powder of collagen is blended, it will not be able to exhibit a high level of dirt removal function unless a proteolytic enzyme agent is also blended.

[0079] [Test 4. Moisture retention function test] This test examined how the presence or absence of plant-derived carbonized powder, collagen powder, and proteolytic agent affected the moisturizing function of the skin after cleansing. The test samples were the same as those used in Test 2.

[0080] (Test 4-1. Sample preparation) In this test, the foam and simulated soil were prepared in the same manner as in Test 3.

[0081] (Test 4-2. Test Method) Six test areas, each 2 cm square, were set up on the inside of the forearms of five subjects. 1 g of simulated soil was placed on each test area and allowed to air dry for 20 minutes. After air drying, each sample was placed on the area to cover the simulated soil and left for 1 minute. After leaving the area, the forearms were towel-dried under 40°C water, and the skin condition of the test areas was evaluated on a 5-point scale. The criteria for moisturizing function are shown in Table 4. [Table 4]

[0082] (Test 4-3. Results) The results of this test are shown in Figure 4. Figure 4 shows that Samples B and C have better moisturizing properties than Sample A. Sample C in particular is superior to the other samples. Additionally, Samples D and F had higher moisturizing properties than Samples A and E, but lower moisturizing properties than Samples B and C.

[0083] These results indicate that the moisturizing function of a cleanser containing a protease can be enhanced by incorporating a collagen powder.

[0084] It is particularly interesting to note from the results of Samples D and F that even when collagen powder is formulated, it does not exhibit sufficient moisturizing function unless it is coexistent with a protease. The inventors believe that this is due to the hydroxyproline produced by hydrolysis of the proteolytic components of the collagen in the collagen powder.

[0085] Therefore, the results of Tests 1 to 4 show that the cleanser of the present invention, which contains a protease, a carbonized plant powder, and a collagen powder, can provide a cleanser that functions at high levels of both cleansing and skin-conditioning effects, and that maintains its cleansing and skin-conditioning effects even after a long storage period.

Claims

[Claim 1] A cleaning agent that passes through a 20-1000 mesh sieve, which is a blend of a proteolytic enzyme agent consisting of crushed bacteria or yeast containing protein digestive enzymes, 0.01-20% by weight of bamboo charcoal powder that passes through a 50-180 mesh sieve, and 0.01-20.5% by weight of porcine collagen powder that passes through a 20-200 mesh sieve, The collagen powder is a cleansing agent characterized in that the total content of proline and hydroxyproline is 15 to 25% by weight.

Citation Information

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