Observation System

Abstract

To provide an observation system enabling, in observation of an observation object, work of preparing the observation object into an observable state to be performed quickly and easily, as well as, enabling work efficiency and observation accuracy relating to the observation to be easily enhanced.SOLUTION: Provided is an observation system including: a first sheet; arrangement means for arranging an observation object on the first sheet; observation means for observing the observation object arranged on the first sheet; and a sheet movement mechanism for moving the first sheet. The sheet movement mechanism is provided with an elastic body on an upstream side in a movement direction of the first sheet, and keeps tension of the first sheet constant. According to the present invention, when observing the observation object arranged on the sheet, by using the elastic body to keep the tension of the sheet constant at the time of the sheet movement and the observation, the observation system prevents strain or deflection of the sheet with simple means and enables observation accuracy to be enhanced.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an observation system. [Background technology]

[0002] In recent years, advances in information processing and observation techniques have made it possible to acquire and analyze large amounts of data. As a result, it has become increasingly important to acquire and analyze observation data on various objects, compile it into a database, and make effective use of the data.

[0003] In particular, when obtaining observation data through microscopic observation, it is now possible to obtain not only image data relating to the shape of the object being observed, but also measurement data in combination with various optical analysis means, and the amount of information that can be used as observation data has increased dramatically.

[0004] While the technology for acquiring and analyzing various observation data has improved, the preparation of specimens (samples) to prepare the objects for observation in a state suitable for observation is largely done manually, and as the number of objects to be observed increases, the labor costs and time required for preparing specimens for observation become significant.In order to create a database of observation data and to effectively utilize the data, it is necessary to quickly perform the work related to the observation of large amounts of objects, so improvements in the efficiency of sample preparation for observation are being considered.

[0005] For example, Patent Document 1 describes an apparatus for automatically processing objects to be observed (biological specimens) that includes a slide tray for holding slides on which the objects to be observed are placed, a device for transporting the slide tray, a fluid module for supplying reagents, and an automatic cover glass attachment device. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Application Laid-Open No. 2013-92532 Summary of the Invention [Problem to be solved by the invention]

[0007] As described in Patent Document 1, it is known to use a cover glass and a slide glass to automate the preparation of a so-called preparation for rapid observation of a plurality of observation objects. However, preparation of a slide generally requires complicated steps as sample preparation operations, starting with placing the object to be observed on a glass slide, followed by supplying reagents, placing a cover glass, and transporting the sample to the microscope stage, etc. Therefore, although the automation of slide preparation described in Patent Document 1 makes it possible to significantly reduce labor costs, there remain issues regarding the efficiency of mass sample preparation and sample observation, such as the need to replace the prepared sample after sample observation when using a slide.

[0008] Furthermore, with the recent advances in medical and biological technology, there has been an increase in the number of cells and microorganisms being used as observation subjects. In this case, there is a trend toward observing living organisms such as living cells and living microorganisms (hereinafter simply referred to as "living organisms"), rather than dead cells or dead microorganisms, and obtaining observation data from them. With conventional specimen formats such as slides, there is a risk that the state and position of the object being observed may change during the process of preparing the slide and moving it to the microscope stage, which further complicates the process of acquiring observation data and makes it difficult to obtain accurate observation data. Furthermore, when observing living organisms, sample preparation using multiwell plates with multiple wells (holes) is also known, but this method presents the problem of difficulty in immobilizing the living organism and making observation difficult. Therefore, new observation techniques that do not rely on slides or multiwell plates used in conventional sample preparation are needed.

[0009] The inventors have already discovered an observation system that enables sample preparation and sample observation without using slides or multi-well plates. By placing the objects to be observed on a sheet and moving the sheet, sample preparation and observation can be easily carried out continuously, thereby further improving the work efficiency of observing large numbers of objects to be observed. On the other hand, when observing an object placed on a sheet, as in this observation system, distortion and bending of the sheet during sheet movement significantly affect the efficiency and accuracy of the observation. For this reason, it is expected that the output of the drive mechanism related to the sheet movement will be adjusted, but there are issues such as the difficulty of controlling the output adjustment itself and the complexity and expense of the device required for control, and therefore a simpler means is desired.

[0010] An object of the present invention is to provide an observation system that makes it quick and easy to prepare an observation object so that it can be observed, and that can easily improve the efficiency and accuracy of the observation work. [Means for solving the problem]

[0011] As a result of thorough investigation into the above-mentioned problems, the inventors discovered that by placing the object to be observed on a sheet and performing the observation, and by providing an elastic body as a mechanism for appropriately controlling the tension of the moving sheet, it is possible to speed up and simplify the work involved in sample preparation of the object to be observed, and to easily improve the work efficiency and accuracy of the observation, and thus completed the present invention. That is, the present invention provides the following observation system.

[0012] The observation system of the present invention, which solves the above problem, comprises a first sheet, a placement means for placing an object to be observed on the first sheet, an observation means for observing the object to be observed placed on the first sheet, and a sheet moving mechanism for moving the first sheet, and the sheet moving mechanism is characterized by having an elastic body provided upstream in the movement direction of the first sheet, thereby maintaining a constant tension on the first sheet. The observation system of the present invention allows for the preparation and observation of specimens without the need for preparation of slides using a cover glass and a slide glass by placing the specimen on a sheet and performing the observation in this state. Furthermore, by moving the sheet, it becomes easy to continuously perform specimen preparation and specimen observation. Furthermore, by using an elastic body to maintain constant tension in the sheet during sheet movement and observation, distortion and bending of the sheet can be suppressed by a simple means, thereby improving the accuracy of observation. This speeds up and simplifies the process of preparing specimens for observation, and also makes it possible to easily improve the work efficiency and observation accuracy involved in observing a large number of specimens.

[0013] In one embodiment of the observation system of the present invention, the elastic body is a constant force spring. A constant force spring is a type of elastic body (spring) that can generate a constant force regardless of the amount of unwinding of the spring. Therefore, this feature allows for easy control of the load applied to the sheet during sheet movement, facilitating stable sheet movement and sheet tension control. Furthermore, by using a constant force spring, excessive load is not applied to the sheet, reducing the load caused by frictional forces on the sheet. This suppresses sheet temperature rise and deformation, minimizing the impact on the placed observation object itself, as well as the impact on the observation work and observation accuracy.

[0014] Furthermore, one embodiment of the observation system of the present invention comprises a second sheet that is superimposed on the first sheet after the object to be observed has been placed on the first sheet, the observation means observes the object to be observed while fixed between the first sheet and the second sheet, and the sheet movement mechanism is characterized by providing an elastic body upstream in the movement direction of the first sheet and / or the second sheet, and maintaining constant tension in the first sheet and / or the second sheet. According to this feature, since the observation object is fixed between two sheets, it is easy to prepare a sample suitable for observation and observe the sample. In addition, it does not apply an external force stronger than necessary to the observation object. This makes it easy to handle living organisms such as living cells and living microorganisms as the observation object. Therefore, it is possible to improve the work efficiency of observing a large number of observation objects, especially living organisms, and to improve the work efficiency of database creation of observation data and effective use of data. [Effects of the Invention]

[0015] According to the present invention, an observation system can be provided that makes it quick and easy to prepare an observation object so that it can be observed, and can easily improve the efficiency and accuracy of the observation work. [Brief explanation of the drawings]

[0016] [Figure 1] FIG. 1 is a schematic explanatory diagram of an observation system according to a first embodiment of the present invention. [Figure 2] FIG. 10 is a schematic explanatory diagram showing another example of the sheet moving mechanism in the observation system according to the first embodiment of the present invention. [Figure 3] FIG. 10 is a schematic explanatory diagram of an observation system according to a second embodiment of the present invention. [Figure 4] FIG. 10 is a schematic explanatory diagram showing another aspect of the observation system according to the second embodiment of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0017] Hereinafter, an embodiment of an observation system according to the present invention will be described in detail with reference to the drawings. The observation system described in the embodiment is merely an example for explaining the observation system according to the present invention, and is not limited to this.

[0018] In the observation system of the present invention, the object to be observed is not particularly limited, and can be anything that requires the accumulation and analysis of observation data through observation in various fields. Furthermore, the form of the object to be observed may be anything that can be placed on a sheet, and may be in the form of a liquid, solid, or solution containing the object to be observed. Recent advances in medicine and biology have increased the importance of accumulating and analyzing observation data related to cells, microorganisms, and the like. Therefore, the observation objects of the present invention include cells, microorganisms, and other objects for which the collection of observation data is considered important in medicine and biology. In particular, it is preferable to use living organisms such as live cells and living microorganisms, which are difficult to observe using conventional specimen preparations using slides, as the observation objects. Other examples of the observation objects of the present invention include powders and thin solid flakes of metals and rocks, as well as fine particles of organic and inorganic substances. In the following embodiments, the observation object will be mainly described as an aqueous solution containing a living organism, but the observation object is not limited to this.

[0019] [First embodiment] (Observation System) FIG. 1 is a schematic explanatory diagram showing the structure of an observation system according to a first embodiment of the present invention. As shown in Fig. 1, the observation system 1A in this embodiment comprises a first sheet 2a, arrangement means 3 for arranging an observation object S, observation means 4, and a sheet moving mechanism 5. In Fig. 1, the dashed arrows indicate connections that enable data input / output and control.

[0020] In the observation system 1A of this embodiment, an observation target S is placed on a first sheet 2a by the placement means 3, and then observation is performed in this state using the observation means 4. That is, the observation system 1A of this embodiment performs observation using the first sheet 2a on which the observation target S is placed as a sample, instead of a conventional preparation consisting of a cover glass and a slide glass. Furthermore, by providing a sheet moving mechanism 5 that moves the first sheet 2a, it becomes possible to perform the entire process from sample preparation to sample observation as a single series of operations. This simplifies the work of preparing and replacing samples in conventional observations using preparations, and makes it possible to improve the efficiency of the observation work.

[0021] Each component of the observation system 1A will be described in detail below.

[0022] (First sheet) The first sheet 2a is for supporting the observation object S, on which the observation object S is placed.

[0023] The first sheet 2a may be any sheet on which the observation object S can be placed and which can be observed by the observation means 4, and there are no particular limitations on the material or shape. The first sheet 2a may have any shape as long as it has a width that is sufficient to ensure the field of view of the observation means 4 described below. The first sheet 2a may be, for example, a rolled film, which can facilitate the movement of the sheet by the sheet moving mechanism 5 described below.

[0024] Examples of materials for the first sheet 2a in this embodiment include materials that are light-transmitting, insoluble in water, and impermeable to water. Resistance to organic solvents is also preferable. Examples of such materials include resin films and glass films. For example, by using inexpensive resin films that are widely available on the market, such as polyethylene film, polypropylene film, and polyethylene terephthalate (PET) film, the running costs associated with observation can be reduced. Polyethylene film is known to have high transmittance to terahertz waves, and is particularly suitable for use when electron beam observation is used as the observation means 4. Furthermore, it is more preferable that the first sheet 2a be made of a material that is not only light-transmitting but also has little autofluorescence or light absorption. This can reduce the effect on observation with the observation means 4. Examples of such materials include resin films with little light absorption, such as cycloolefin films, and glass films. In particular, glass films are preferably used as a substitute for slides for optical observation. Note that the autofluorescence and light absorption of the first sheet 2a may be addressed by correcting them as background during observation with the observation means 4. Furthermore, the first sheet 2a is preferably resistant to various sterilization processes (electron beam sterilization, ultraviolet sterilization, heat sterilization, etc.). This makes it possible to prevent contamination with bacteria and microorganisms other than the object of observation when a living organism is used as the observation object S. Furthermore, when a living organism is used as the observation object S, the first sheet 2a may be selected or made oxygen permeable / non-permeable. For example, if the living organism is anaerobic, it is preferable to select a material that is oxygen non-permeable for the first sheet 2a. On the other hand, if the living organism is aerobic, it is preferable to select a material that is oxygen permeable for the first sheet 2a. This makes it possible to control the effect of oxygen on the living organism during observation work.

[0025] The first sheet 2a may be subjected to a surface treatment, but the type of surface treatment is not particularly limited. For example, one example of surface treatment is a treatment related to surface properties against water, such as hydrophilic treatment or water-repellent treatment. Note that surface properties against water include those related to hydrophilicity / hydrophobicity, as well as wetting properties and the contact angle of water. At this time, the hydrophilic treatment or water-repellent treatment may be performed on the entire sheet, or the hydrophilic or water-repellent treatment may be performed in a certain pattern. For example, the hydrophilic treatment is performed so that square, rectangular, circular, or elliptical patterns are arranged at regular intervals, and an observation object S made of an aqueous solution is placed on the hydrophilic treated area using the placement means 3 described later. This makes it possible to stably place the observation object S on the surface-treated area.

[0026] Another example of surface treatment is to apply a substance to immobilize the observation object S. For example, if the observation object S is a living organism, the substance to be applied to the first sheet 2a may be an antibody against the living organism, or a protein, peptide, enzyme, nucleic acid, base, sugar chain, phospholipid, glycolipid, etc. This makes it possible to stably position the observation object S at the location where the surface treatment has been performed. Another example of surface treatment is to apply a reagent for observing the observation object S. Examples of such reagents include staining reagents, fluorescent reagents, and various probes. This makes it easier to observe the observation object S at the surface-treated location using the observation means 4, and improves the efficiency of the observation work.

[0027] The first sheet 2a may also incorporate various types of identification information to facilitate identification during observation. Examples of such identification information include size-related identification information such as grids and scale bars, and position-related identification information such as markers provided at predetermined intervals, guides such as codes (numbering, character codes, bar codes, etc.). Methods for incorporating the identification information include printing the identification information on the first sheet 2a in advance or manually writing it in.

[0028] (Arrangement means) The placement means 3 is for placing the observation object S on the first sheet 2a. The placement means 3 in this embodiment is not particularly limited as long as it can place the observation object S. It is preferable to select the placement means 3 according to the form (liquid or solid) of the observation object S.

[0029] A specific example of the placement means 3 is one that includes a dispensing unit 30, a supply unit 31 that supplies the object to be observed S to the dispensing unit 30, and a control mechanism 32 that controls the angle and position of the dispensing unit 30, as shown in Figure 1.

[0030] The dispensing unit 30 is used when the observation object S is a liquid, and dispenses the observation object S by dropping it onto the first sheet 2a. The dispensing unit 30 may be any device having a structure suitable for dripping and discharging liquid, such as a tip or a syringe. The material of the dispensing unit 30 is not particularly limited and can be selected depending on the properties of the observation object S. Examples of materials for the dispensing unit 30 include plastic, metal, and glass.

[0031] It is preferable that the dispensing unit 30 has a replaceable structure. This allows the dispensing unit 30 to be replaced depending on the type of observation object S, changes in observation conditions, etc., and makes it possible to prevent contamination such as the mixing of other observation objects S during observation. In addition, from the perspective of preventing contamination, the dispensing unit 30 can be made washable, and washed every time the type of observation object S is changed. From the viewpoint of improving the efficiency of observation, it is preferable to make the dispensing unit 30 replaceable. In this case, an inexpensive plastic tip can be used as the dispensing unit 30 and replaced in a disposable manner. This allows the next observation object S to be placed with the short operation of simply replacing the tip, making it possible to perform observations in a substantially continuous state. On the other hand, when a metal or glass tip or syringe is used as the dispensing unit 30, it is preferable to provide a means for cleaning the dispensing unit 30. This allows the dispensing unit 30 to be used repeatedly, thereby reducing the cost of replacing the dispensing unit 30.

[0032] The supply unit 31 supplies the observation object S to the dispensing unit 30. The supply unit 31 may be any unit capable of supplying the observation object S to the dispensing unit 30, and the specific structure thereof is not particularly limited. The supply unit 31 in this embodiment may include, for example, a storage unit 31a for storing the observation object S, and a supply line 31b and a pump 31c for supplying the observation object S to the dispensing unit 30, as shown in FIG.

[0033] The storage section 31a in the supply unit 31 is not particularly limited as long as it can store the observation object S (liquid). Examples include a water tank, a flask, a beaker, and a tube. When storing a solution containing the observation object S, the storage section 31a may be provided with a stirring mechanism. This increases the dispersibility of the observation object S in the solution, and prevents only the solution not containing the observation object S from being supplied to the dispensing unit 30.

[0034] The supply line 31b and the pump 31c are not particularly limited as long as they are capable of transporting the observation object S (liquid). For example, the supply line 31b may be a tube made of resin or metal. The pump 31c may be a pump capable of accurately supplying a predetermined volume, such as various metering pumps or a pump equipped with a flow control valve. This makes it easier to set conditions for the placement of the observation object S, thereby improving the efficiency of the observation work.

[0035] The control mechanism 32 is for controlling the angle and position of the dispensing unit 30 . The control mechanism 32 may include a support connected to the dispensing unit 30 and a drive mechanism that drives the support along the X, Y, and Z axes and the rotation axis. The drive mechanism may be operated manually, or may be provided with a control unit that controls it by inputting numerical values ​​or automatically controls it using a program, etc. This makes it possible to adjust the angle and position of the dispensing unit 30 relative to the first sheet 2a and place the observation object S at any position on the first sheet 2a.

[0036] One example of position control of the dispensing unit 30 by the control mechanism 32 is to move the dispensing unit 30 to a position away from the first sheet 2a when the observation work is temporarily stopped and then restarted, such as during or immediately after cleaning of the dispensing unit 30, or immediately after changing the type of observation object S, and then move the dispensing unit 30 onto the first sheet 2a and place the observation object S. This prevents waste liquid unsuitable for observation from being placed on the first sheet 2a, making it possible to improve the efficiency of the observation work.

[0037] The arrangement means 3 is not limited to the configuration shown in Fig. 1. For example, a plurality of arrangement means 3 may be provided. When a plurality of arrangement means 3 is provided, what is arranged on the first sheet 2a by the arrangement means 3 is not only the observation object S, but also a reagent required for observing the observation object S. For example, when the observation object S is a solution containing a living organism, a staining reagent that is widely used in observing living organisms can be placed by the arranging means 3. Furthermore, the objects placed by the arranging means 3 other than the observation object S are not limited to reagents (staining reagents) necessary for observing the observation object S, but include chemical substances (reagents) for observing the effects of environmental changes on the observation object S. For example, when the observation object S is a solution containing a living organism, a solution with a high salt concentration or pH can be placed by the arranging means 3 and mixed with the observation object S to observe the effects of the salt concentration or pH on the living organism. This makes it possible to quickly and easily obtain observation data accompanying environmental changes on the observation object S.

[0038] As another example of the arrangement means 3, a dispensing tool such as a pipette may be used as the dispensing unit 30 and the supply unit 31, and the observation objects S may be arranged manually. This is particularly useful when the number of observation objects S to be arranged is small, such as when considering the setting of observation conditions, such as the material of the first sheet 2a and the selection of surface treatment conditions. Furthermore, the arrangement means 3 may be a pipette or syringe pump that automates the process from sucking up to discharging the observation objects S. This makes it easy to automate the arrangement process, thereby improving the work efficiency related to observation. Furthermore, by performing position control using the control mechanism 32, it is possible to switch the object to be sucked up as the observation object S, and automatically and continuously switch between multiple observation objects S.

[0039] Another example of the placement means 3 is to provide a distribution unit equipped with a pickup device such as tweezers instead of the dispensing unit 30. This makes it possible to place a solid flake as the observation object S on the first sheet 2a. The pickup device in the distribution unit may have any structure that can grasp and carry the solid flake as the observation object S, and examples include a pickup device that has a structure that clamps the solid flake, like ordinary tweezers, or a pickup device that has a suction structure, like suction tweezers.

[0040] (Method of observation) The observation means 4 is for observing the observation object S placed on the first sheet 2a. The observation means 4 is not particularly limited as long as it can acquire observation data of the observation object S. Examples include image acquisition means for acquiring information related to the shape of the observation object S, and optical analysis means, radiation analysis means, magnetic field analysis means, and electron beam diffraction means for acquiring information based on various analyses of the observation object S. The observation means 4 in this embodiment may be configured as a microscope. In particular, it is preferable to use an optical microscope. This allows for detailed observation by obtaining a magnified image of the observation object S, and also makes it easy to combine with various analytical means.

[0041] The observation means 4 may include an objective lens 40, an eyepiece, and a reflector (light source) that are commonly used in observations using an optical microscope. It is also preferable to provide an image acquisition means (such as a CCD camera or a CMOS camera) for image acquisition instead of the eyepiece, thereby enabling automatic collection of image data. This improves the efficiency of work related to the acquisition and analysis of observation data. Note that FIG. 1 illustrates only the objective lens 40 as the observation means 4, and omits other components. In the observation system 1A of this embodiment, as shown in FIG. 1, a first sheet 2a held by a sheet moving mechanism 5 is placed below the objective lens 40, and the observation object S placed on the first sheet 2a is observed, so it is not necessary to provide a stage as in a general optical microscope. 1 shows the structure of an upright microscope in which the objective lens 40 is located above the observation object S, but the present invention is not limited to this and may also use the structure of an inverted microscope in which the objective lens 40 is located below the observation object S. Furthermore, a plurality of objective lenses 40 may be provided so that the observation object S can be observed from a plurality of directions.

[0042] It is preferable to organize and analyze the observation data obtained by the observation means 4 as necessary. For example, a data storage unit and a data analysis unit that store and analyze data can be provided, and the necessary calculations can be performed by inputting the observation data. This makes it possible to create a database of observation data related to the observation object S and to make effective use of the data.

[0043] (Seat movement mechanism) The seat moving mechanism 5 is for supporting and moving the first seat 2a. The sheet moving mechanism 5 includes an unwinding roll 50a as an unwinding section 50 that unwinds and supplies the first sheet 2a, and a winding roll 51a as a winding section 51 that winds and recovers the first sheet 2a, and a drive section (not shown) is provided for driving the winding section 51 (winding roll 51a) to rotate.

[0044] The sheet moving mechanism 5 preferably has a speed adjusting function 52 that adjusts the moving speed of the sheet. For example, the speed adjusting function 52 may be provided by providing a rotation drive control unit 52a that can record the number of rotations or measure the winding diameter and control the number of rotations to a drive unit related to the rotation drive of the winding unit 51. This makes it possible to adjust the winding (recovery) speed of the sheet, and thereby adjust the moving speed of the sheet.

[0045] The sheet moving mechanism 5 in this embodiment may have a speed adjustment function 52 for adjusting the sheet moving speed, as well as a function for controlling the movement and stopping of the sheet. For example, the drive unit for rotating the winding unit 51 may be controlled to move the first sheet 2a a certain distance or for a certain time, and then stop it for a certain time. Here, this control may be performed by the rotation drive control unit 52a, or a separate control unit may be provided. As a result, when observing the observation object S using the observation means 4, the observation object S can be moved intermittently and observed while the observation object S is stationary, thereby improving the accuracy of the observation. Further, another example of control related to the sheet movement in the sheet moving mechanism 5 is to switch between different operation modes, such as an operation of repeatedly moving and stopping the sheet as an operation mode for observing the observation object S, or an operation of moving the sheet at a constant speed as an operation mode for arranging the observation object S. This allows the observation conditions in the observation means 4 to be set more freely, and also makes it possible to more accurately arrange the observation object S.

[0046] The sheet moving mechanism 5 in this embodiment is provided with tension control means 53 for controlling the tension of the first sheet 2a. The tension control means 53 in this embodiment has the function of appropriately controlling the tension of the first sheet 2a and includes an elastic body 53a. By using the elastic body 53a as the tension control means 53, it becomes possible to appropriately control the tension of the first sheet 2a using a simple and inexpensive means without relying on the control of the rotation drive control unit 52a. It is preferable that the tension control means 53 also has the function of supporting the first sheet 2a. This allows the first sheet 2a to move stably.

[0047] The elastic body 53a is provided upstream of the observation means 4 in the moving direction of the first sheet 2a, and may be any body capable of controlling the tension of the first sheet 2a by applying a predetermined load to the first sheet 2a, and it is particularly preferable to use a constant force spring. The structure of the constant force spring will be described later.

[0048] Here, the constant force spring is a type of elastic body (spring) that can obtain a constant force regardless of the amount of unwinding of the spring. Therefore, by using a constant force spring as the elastic body 53a, the load applied to the first sheet 2a during movement can be easily controlled to be constant, facilitating stable sheet movement and sheet tension control. Furthermore, by using a constant force spring, an excessive load is not applied to the first sheet 2a, thereby reducing the load due to frictional force acting on the first sheet 2a. This prevents the first sheet 2a from increasing in temperature or deforming due to frictional force generated by the tension control means 53, thereby minimizing the impact on the placed observation object S itself and the impact on the observation work and observation accuracy.

[0049] 1, an example of the tension control means 53 in this embodiment is one in which an elastic body 53a is provided so as to contact the first sheet 2a from below the first sheet 2a, and a load from the elastic body 53a is applied to the first sheet 2a perpendicular to the movement direction (X direction) of the first sheet 2a and from the lower side of the sheet (lower side in the Z direction), thereby controlling the tension of the first sheet 2a. In this case, by appropriately selecting the type and performance of the elastic body 53a used and adjusting the load applied to the first sheet 2a, the tension of the first sheet 2a can be maintained at a state suitable for observation. At this time, in order to apply an appropriate load from the elastic body 53a to the first sheet 2a, a mechanism for applying a load (load applying mechanism) to the elastic body 53a itself may be provided. An example of such a load applying mechanism is a solenoid that performs linear reciprocating motion (pushing and pulling) using electromagnetic force. This makes it possible to provide a simpler and less expensive means for controlling the tension of the sheet, without requiring complex control compared to the control of the rotation drive control unit 52a.

[0050] In order to protect the first sheet 2a at the location where the elastic body 53a and the first sheet 2a come into contact with each other, the tip of the elastic body 53a may be processed.

[0051] The tension control means 53 may be provided with a configuration other than the elastic body 53a. For example, as shown in FIG. 1, a pressure-receiving member 53d that receives a load from the elastic body 53a may be provided at a location facing the elastic body 53a so as to sandwich the first sheet 2a. The pressure-receiving member 53d may be any member that can receive the load from the elastic body 53a and sandwich the first sheet 2a while protecting it, and there are no particular limitations on its structure or material. In this case, depending on the location of the pressure-receiving member 53d, it may also be possible to support tension control of the first sheet 2a due to the load from the elastic body 53a. The pressure-receiving member 53d may also serve as a support mechanism 53b, which will be described later.

[0052] FIG. 2 is a schematic explanatory diagram showing another aspect of the sheet moving mechanism 5 in the observation system 1A of this embodiment. Here, FIG. 2(A) is a schematic explanatory diagram enlarging the parts related to the observation means 4 and the tension control means 53 in the observation system 1A, and some of the structure related to the sheet moving mechanism 5 other than the placement means 3 and the tension control means 53 is not shown. Also, FIGS. 2(B) and 2(C) are schematic explanatory diagrams showing an example of tension control by the tension control means 53, and only show the parts particularly related to tension control, and some of the other structure is not shown. Note that explanations of the same configuration as shown in FIG. 1 will be omitted.

[0053] As shown in Figure 2(A), another example of the tension control means 53 in this embodiment is one in which, instead of the pressure-receiving member 53d, a support mechanism 53b having the function of supporting the first sheet 2a is provided, and the elastic body 53a applies a load to the support mechanism 53b, which in turn applies a load to the first sheet 2a, thereby controlling the tension of the first sheet 2a.

[0054] The support mechanism 53b may be any mechanism capable of receiving the load from the elastic body 53a and supporting and controlling the tension of the first sheet 2a. For example, a support roller 530b equipped with a vertical drive mechanism capable of moving in the vertical direction UD1 may be provided. In this case, the support roller 530b preferably has a rotation shaft and rotates in a rotation direction R3 along the sheet movement direction as the first sheet 2a moves. This allows for smooth movement of the first sheet 2a in addition to supporting the first sheet 2a. The arrangement and number of support mechanisms 53b are not particularly limited. For example, as shown in FIG. 2(A), in addition to the support mechanism 53b interlocking with the elastic body 53a, another support mechanism 53b may be provided at a position opposite the first sheet 2a across the first sheet 2a, functioning as a pressure-receiving member 53d.

[0055] Based on Figure 2, an example of sheet tension control by the tension control means 53 of this embodiment will be described below, in which an elastic body 53a and a support mechanism 53b are combined, a constant force spring 530a is used as the elastic body 53a, and a support roller 530b is used as the support mechanism 53b.

[0056] In this embodiment, the constant force spring 530a serving as the elastic body 53a includes drums D1 and D2 and a plate-shaped spring 531a. Rotation of the drum D1 unwinds and rewinds the plate-shaped spring 531a, providing a constant output. The means for transmitting the output from the elastic body 53a to the support mechanism 53b is not particularly limited. For example, as shown in FIG. 2, the rotational axes of the drum D1 and the support mechanism 53b (support roller 530b) may be coaxially arranged, and a connecting shaft 53c may be provided to connect these rotational axes. This allows the rotation of the support roller 530b to be linked to the rotation of the drum D1 in the constant force spring 530a. Another example of a means for transmitting the output from the elastic body 53a to the support mechanism 53b is to use a timing belt or gear instead of the connecting shaft 53c that connects the drum D1 and the support mechanism 53b (support roller 530b).

[0057] Hereinafter, the sheet tension control by the tension control means 53 in this embodiment will be described mainly with reference to FIGS. 2(B) and 2(C). FIG. 2B shows an example of tension applied to the first sheet 2a by the tension control means 53. As shown in FIG. 2B, the support roller 530b and the first sheet 2a are arranged so as to be in contact with each other. By moving the first sheet 2a in the X direction, the support roller 530b rotates in a rotational direction R3. The drum D1 of the constant force spring 530a is linked to the rotation of the support roller 530b via the connecting shaft 53c. As the drum D1 rotates in the rotational direction R1, the plate-shaped spring body 531a is unwound from the drum D2. At this time, the constant force spring 530a applies a force to the support roller 530b in the direction opposite to the rotational direction R3. In other words, by applying a load to the support roller 530b in the rotational direction R3, a load is applied to the first sheet 2a, thereby controlling the tension of the first sheet 2a. On the other hand, Figure 2(C) shows an example of the tension control means 53 alleviating the tension load on the first sheet 2a. As shown in Figure 2(C), by moving the support roller 530b upward in the Z direction in the up-down direction UD1 and separating the first sheet 2a from the support roller 530b, a force related to rotation is no longer applied to the support roller 530b. This alleviates the tension load applied to the first sheet 2a. At this time, the drum D1 of the constant force spring 530a rotates in the rotation direction R2, causing the plate-shaped spring body 531a to be wound around the drum D2.

[0058] The power for rotating the drum D1 in the constant force spring 530a is not particularly limited. 2, in addition to linking the rotational drive of the support roller 530b via the connecting shaft 53c, the rotational shaft of the drum D1 and the solenoid can be connected with a linear member such as a wire to forcibly rotate the drum D1 without relying on the rotation of the support roller 530b caused by the movement of the first sheet 2a. In this case, by converting the reciprocating motion of the solenoid into the rotational drive of the rotational shaft of the drum D1, it becomes possible to unwind / wind the plate-shaped spring body 531a. Another example is to provide a drive unit that rotates the rotation shaft of the drum D1. In this case, the torque required is smaller than that of a drive unit that rotates the retraction unit 51, and the control of the drive unit is not complicated. Therefore, even when a drive unit that rotates the elastic body 53a is provided, it is possible to provide a simpler and less expensive means for controlling the tension of the sheet compared to the control by the rotation drive control unit 52a.

[0059] In the observation system 1A of this embodiment, it is sufficient that the tension of the first sheet 2a is appropriately controlled when observing the observation object S or when moving the first sheet 2a, and there is no need to constantly control the tension. For example, when placing the observation object S or when replacing the observation object S, there are cases where sheet tension control is not necessary. Therefore, the tension control means 53 in this embodiment does not need to constantly apply a load by the elastic body 53a, and the load applying mechanism such as a solenoid may be controlled to be turned on and off as needed. Furthermore, when a constant force spring 530a is used as the elastic body 53a, there is generally a certain limit to the amount of unwinding of the constant force spring 530a. However, with the tension control means 53 of this embodiment, there are regular periods when the load from the elastic body 53a (tension control of the first sheet 2a) is not required, so it is possible to perform an operation to wind up the constant force spring 530a, as shown in Figure 2(C). Therefore, there is no limit to the amount of unwinding of the constant force spring 530a, and it can be used as the tension control means 53 of this embodiment. When winding the constant force spring 530a, a load is generated in the opposite direction to that generated when unwinding. Therefore, as shown in FIG. 2(C), if the constant force spring 530a and the support mechanism 53b are completely connected by the connecting shaft 53c, it is necessary to separate the support mechanism 53b and the first sheet 2a using a vertical drive mechanism that is movable in the vertical direction UD1. However, this is not limited to this. For example, instead of separating the support mechanism 53b and the first sheet 2a, a structure that allows the connection between the support mechanism 53b and the constant force spring 530a to be temporarily disconnected may be provided. More specifically, the connecting shaft 53c and the support mechanism 53b (or the constant force spring 530a) may be connected so as to be separable, or a clutch mechanism may be used to connect the support mechanism 53b (support roller 530b) and the constant force spring 530a (drum D1) instead of the connecting shaft 53c. As a result, when the constant force spring 530a is wound up, the position of the support mechanism 53b does not change, and it is possible to continue supporting the first seat 2a.

[0060] The tension control means 53 may control the tension after determining the tension in the first sheet 2a. For example, a tension detection unit may be provided between the unwinding unit 50 and the winding unit 51. This allows for more precise control of the tension in the first sheet 2a, making it possible to reduce distortion and bending of the first sheet 2a and improve the accuracy of observation. It also makes it possible to detect when a defect occurs in the elastic body 53a, making it difficult to perform appropriate tension control.

[0061] The support mechanism 53b may be provided independently of the elastic body 53a. For example, the support mechanism 53b alone may be provided at a location separate from the elastic body 53a, on the upstream and / or downstream side in the movement direction of the first sheet 2a with respect to the objective lens 40 of the observation means 4. This makes it possible to support the first sheet 2a regardless of whether tension control by the elastic body 53a is performed or not.

[0062] The support mechanism 53b may also be provided with a function for adjusting the sheet movement speed. For example, a measuring unit that measures the number of rotations or rotation speed of the support roller 530b and a rotation drive control unit that can control the rotation drive of the support roller 530b may be provided. The first sheet 2a moves in accordance with the rotation drive of the support roller 530b. Therefore, in addition to the speed adjustment function 52, the sheet movement speed can also be adjusted by controlling the rotation drive of the support roller 530b.

[0063] In the observation system 1A of this embodiment, the observation object S is placed on the first sheet 2a, and observation is performed in this state, thereby enabling sample preparation and sample observation without the need to prepare a slide using a cover glass and a slide glass. Furthermore, by keeping the tension of the first sheet 2a constant via tension control means 53 using elastic body 53a when moving the first sheet 2a or observing the observation object S, it becomes easy to continuously perform sample preparation and sample observation, and it becomes possible to suppress distortion and bending of the sheet using a simple means and improve observation accuracy. This makes it possible to quickly and easily prepare the observation object so that it can be observed, and also to easily improve the work efficiency and observation accuracy involved in observing a large number of observation objects. In addition to the tension control means 53, the observation system 1A in this embodiment may be provided with various means and mechanisms for improving the efficiency of observation-related work and the observation accuracy.

[0064] Furthermore, the observation system 1A in this embodiment is preferably provided with a control means C that adjusts various operations in the placement means 3, observation means 4, and sheet moving mechanism 5. More specifically, as shown in FIG. 1, a control unit C1 is provided that is connected to the placement means 3, observation means 4, and sheet moving mechanism 5 and enables input / output and control of data. A control unit C1 may be provided for each of the various means, but as shown in FIG. 1, it is preferable to connect multiple means to one control unit C1 and enable comprehensive control. This makes it possible to simply and efficiently adjust the various operations and perform operations related to the adjustments. Examples of operations to be controlled by the control unit C1 include operations related to the operation of the observation means 4, operations related to the acquisition and storage of observation data by the observation means 4, and the arrangement of the observation target S on the sheet (first sheet 2a) by drive control of the supply unit 31 and control mechanism 32 of the arrangement means 3 based on data related to the observation results by the observation means 4, as well as sheet operations such as operations directly related to the horizontal movement of the sheet, such as sheet movement speed control and sheet movement / stopping by the speed adjustment function 52 (rotational drive control unit 52a) and rotational drive control of the support mechanism 53b in the sheet movement mechanism 5, and sheet operations related to assisting the horizontal movement of the sheet, such as sheet tension control by drive control of the tension control means 53. Furthermore, it is preferable to control not only individual operations of each means, but also operations resulting from a combination of multiple operations of each means. For example, it is possible to control a series of operations such as acquiring observation data by the observation means 4 in accordance with the timing when the sheet movement mechanism 5 stops the movement of the sheet, or to control a series of operations related to the placement means 3, observation means 4, and sheet movement mechanism 5 for each observation object S for multiple observation objects S. This makes it possible to improve the accuracy of the observation data acquired by the observation means 4 and improve the work efficiency related to observation.

[0065] [Second embodiment] Fig. 3 is a schematic explanatory diagram showing an observation system according to a second embodiment of the present invention, and Fig. 4 is a schematic explanatory diagram showing another aspect of the observation system according to the second embodiment of the present invention. The observation system 1B according to the second embodiment is the same as the observation system 1A according to the first embodiment, except that it includes a second sheet 2b that is superimposed on the first sheet 2a, and the observation means 4 observes the observation object S fixed between the first sheet 2a and the second sheet 2b. Note that a description of the same components as those in the first embodiment will be omitted.

[0066] In the observation system 1B of this embodiment, the observation object S is placed on a first sheet 2a using the placement means 3, and then a second sheet 2b is placed on top of it to fix the observation object S between the first sheet 2a and the second sheet 2b, and observation is performed in this state using the observation means 4. That is, instead of a conventional preparation consisting of a cover glass and a slide glass, the overlapping first sheet 2a and second sheet 2b in this embodiment is used as a sample for observation. In addition, by moving the first sheet 2a and the second sheet 2b using the sheet moving mechanism 5, it is possible to perform the entire process from sample preparation to sample observation as a single operation. This simplifies the work of preparing and replacing samples in conventional observations using preparations, and improves the efficiency of the observation work.

[0067] The second sheet 2b in the observation system 1B of this embodiment is superimposed on the first sheet 2a on which the observation object S is arranged, thereby fixing the observation object S.

[0068] The second sheet 2b can be made of the same material and have the same shape as the first sheet 2a, and there are no particular limitations on the details.

[0069] The first sheet 2a and the second sheet 2b may be made of the same material or different materials, and the thickness of the first sheet 2a and the second sheet 2b may be the same or different.

[0070] Furthermore, when surface treatment is performed on the first sheet 2a and the second sheet 2b, it is preferable that one or some of the first sheet 2a and the second sheet 2b have different surface properties against water. This allows the aqueous solution containing the observation object S to be appropriately spread between the sheets when the second sheet 2b is placed on the first sheet 2a, making it easier to maintain a constant gap between the sheets. This allows the observation object S to be stably fixed and improves the efficiency of observation using the observation means 4.

[0071] In the observation system 1B of this embodiment, when the observation object S is a solution containing a living organism, it is preferable to provide a plurality of placement units 3 and place a staining reagent that is widely used in the observation of living organisms using the placement unit 3. In this case, it is preferable to mix the observation object S and the staining reagent by overlapping the first sheet 2a and the second sheet 2b, rather than supplying the staining reagent directly to the observation object S placed on the first sheet 2a. This makes it possible to suppress contamination in the dispensing unit 30. For example, the observation object S and the staining reagent may be placed adjacent to each other on the first sheet 2a without contacting each other, or the angle of the dispensing unit 30 that places the observation object S may be different from the angle of the dispensing unit 30 that places the staining reagent, and the observation object S and the staining reagent may be placed on the first sheet 2a and the second sheet 2b, respectively.

[0072] After placing the observation object S on the first sheet 2a using the placement means 3, the second sheet 2b is placed on top of it, thereby fixing the observation object S between the first sheet 2a and the second sheet 2b. The means for placing the first sheet 2a and the second sheet 2b on top of each other is not particularly limited. For example, the first sheet 2a and the second sheet 2b may be placed on top of each other using a sheet moving mechanism 5 or a sheet placement adjustment mechanism 6 described later. This makes it easy to automate the work involved in sample preparation.

[0073] The observation means 4 in this embodiment is for observing an observation object S fixed between the first sheet 2a and the second sheet 2b. In the observation system 1B of this embodiment, as shown in Figure 3, two sheets (a first sheet 2a and a second sheet 2b) held by a sheet moving mechanism 5 are placed below the objective lens 40, and the observation object S fixed between the two sheets is observed, so it is not necessary to provide a stage as in a general optical microscope.

[0074] It is preferable to organize and analyze the observation data obtained by the observation means 4 as necessary, similar to the observation system 1A in the first embodiment. For example, a data storage unit and a data analysis unit that store and analyze data may be provided, and the necessary calculation processing may be performed by inputting the observation data. This makes it possible to create a database of observation data related to the observation object S and to make effective use of the data.

[0075] The seat moving mechanism 5 in this embodiment is for supporting and moving the first seat 2a and the second seat 2b. The sheet moving mechanism 5 includes an unwinding section 50 and a winding section 51, each of which includes an unwinding roll 50a that unwinds and supplies the first sheet 2a and a winding roll 51a that winds and collects the first sheet 2a, an unwinding roll 50b that unwinds and supplies the second sheet 2b, and a winding roll 51b that winds and collects the second sheet 2b. A drive unit (not shown) is provided to rotate the winding section 51 (winding rolls 51a, 51b). While FIG. 3 illustrates the winding section 51 including winding rolls 51a, 51b, the present invention is not limited to this. For example, the first sheet 2a and the second sheet 2b unwound from the unwinding section 50 (unwinding rolls 50a, 50b) may be wound and collected by a single winding roll. This simplifies the structure of the device.

[0076] The tension control means 53 in this embodiment has the function of appropriately controlling the tension of either or both of the first sheet 2a and the second sheet 2b, and includes at least one elastic body 53a. Furthermore, the tension control means 53 in this embodiment preferably includes a plurality of vertically movable support rollers 530b as a support mechanism 53b for supporting each sheet (first sheet 2a and second sheet 2b) in addition to controlling the tension of the first sheet 2a and the second sheet 2b. This allows for stable movement of the first sheet 2a and the second sheet 2b. Furthermore, the provision of the support mechanism 53b improves the accuracy of positional adjustment of the overlapping of the first sheet 2a and the second sheet 2b, making it possible to easily adjust the distance between the two sheets. In addition, in Figure 3, the elastic body 53a is shown to be provided on the second sheet 2b side, but this is not limited to this, and it may be provided on the first sheet 2a side, or on both the first sheet 2a side and the second sheet 2b side. Furthermore, there are no particular limitations on the locations and number of support mechanisms 53b. For example, as shown in Fig. 3, support mechanisms 53b may be provided on both the upstream and downstream sides of the objective lens 40 in the observation means 4 in the sheet movement direction and above the sheet (above the second sheet 2b), enabling stable support of the sheet. As another example, support mechanisms 53b may be provided below the sheet (below the first sheet 2a) or on the side of each sheet where the observation target S is placed. Furthermore, the number of support mechanisms 53b may be reduced to simplify the structure.

[0077] Furthermore, an adjustment mechanism 54 for adjusting the distance between the first sheet 2a and the second sheet 2b may be provided separately. The adjustment mechanism 54 may be, for example, provided with a pressing member that presses from above or below a sheet (first sheet 2a or second sheet 2b) near the objective lens 40 of the observation means 4. Note that FIG. 3 shows the adjustment mechanism 54 provided with a pressing member 54a that presses up the first sheet 2a from below. The pressing member 54a is also provided with a mechanism (not shown) that allows it to move up and down. The pressing member 54a preferably has a structure having a space for ensuring an observation field by the observation means 4. A specific example of the pressing member 54a is a cylindrical member having a cavity. This ensures an observation field by the observation means 4 and makes it possible to evenly press up the first sheet 2a.

[0078] In the observation system 1B of this embodiment, the distance and alignment between the first sheet 2a and the second sheet 2b affect the stable fixation of the observation object S and the improvement of observation accuracy. For this reason, the observation means 4 in the observation system 1B of this embodiment may be provided with, instead of the adjustment mechanism 54, a sheet arrangement adjustment mechanism 6 that has means for adjusting the distance and alignment between the first sheet 2a and the second sheet 2b and a function for improving observation accuracy.

[0079] Figure 4 is a schematic explanatory diagram showing the structure of the seat arrangement adjustment mechanism in the observation system 1B of this embodiment. Note that Figure 4 is a schematic explanatory diagram enlarging the parts related to the observation means 4 and the seat arrangement adjustment mechanism 6 in the observation system 1B, and some parts of the arrangement means 3 and the seat moving mechanism 5 are not shown. Furthermore, descriptions of the same components as those shown in Figure 1 will be omitted. The seat arrangement adjustment mechanism 6 in this embodiment includes a fixing jig 61 for attaching the first sheet 2a and the second sheet 2b, and a support part 62 for maintaining the smooth surfaces of the first sheet 2a and the second sheet 2b.

[0080] The fixing jig 61 is a means to which the first sheet 2a and the second sheet 2b are attached, and in particular, for adjusting the distance and alignment of the first sheet 2a and the second sheet 2b with respect to the observation means 4. The fixing jig 61 may be any device that can adjust the distance and alignment of the first sheet 2a and the second sheet 2b with respect to the observation field of the observation means 4, and there are no particular limitations on the specific structure. An example of the structure of the fixing jig 61 will be described later. The material of the fixing jig 61 is not particularly limited. Examples of suitable materials include metal, resin, glass, ceramic, rubber, and wood. Since the first sheet 2a and the second sheet 2b attached to the fixing jig 61 come into contact with the fixing jig 61 during movement, the fixing jig 61 may be painted or surface-treated in consideration of the frictional force (friction coefficient) between the fixing jig 61 and the first sheet 2a and the second sheet 2b. The fixing jig 61 may also be equipped with a static elimination mechanism. This allows the first sheet 2a and the second sheet 2b to move smoothly via the fixing jig 61. The fixing jig 61 may be provided with a function other than that related to the attachment of the first sheet 2a and the second sheet 2b. For example, the surface of the member 610 may be provided with antibacterial, sterilizing, water-repellent, hydrophobic, or other functions. Furthermore, the fixing jig 61 may be provided with a temperature adjustment function, so that it also functions as an incubator or heat panel. This makes it possible to adjust conditions (increase in the population of the observation object S (cultivation) and the activity state of the observation object S) that are particularly suitable for observation when the observation object S is a living organism.

[0081] Furthermore, the support section 62 is a means for maintaining the smooth surfaces of the first sheet 2 a and the second sheet 2 b. The specific structure of the support section 62 is not particularly limited as long as it can maintain the smooth surfaces of the first sheet 2 a and the second sheet 2 b so that the moving first sheet 2 a and the second sheet 2 b do not become distorted or bent, which may affect the holding of the observation object S or the observation accuracy. Here, the support unit 62 may also serve as the tension control means 53, or may be provided separately from the tension control means 53. Furthermore, when the support unit 62 and the tension control means 53 are provided separately, the arrangement of the tension control means 53 and the support unit 62 is not particularly limited. In the following description, it is assumed that the support portion 62 in this embodiment is provided separately from the tension control means 53.

[0082] The material of the support portion 62 is not particularly limited. Examples include a single or multiple materials such as metal, resin, glass, ceramic, rubber, and wood. Since the first sheet 2a and the second sheet 2b come into contact with the support portion 62 when they move, the support portion 62 may be painted or surface-treated in consideration of the frictional force (friction coefficient) between the first sheet 2a and the second sheet 2b. The support portion 62 may also be equipped with a static elimination mechanism. This allows the first sheet 2a and the second sheet 2b to move smoothly via the support portion 62. The support portion 62 may be provided with other functions, such as antibacterial, sterilizing, water-repellent, and hydrophobic functions.

[0083] As shown in FIG. 4, one example of the structure of the fixing jig 61 is a rectangular parallelepiped member 610 having spaces (slits, gaps, etc.) for attaching the first sheet 2a and the second sheet 2b. Also, as shown in FIG. 4, the fixing jig 61 has a hole 611a for providing an area 611 (an area corresponding to the observation field of the observation means 4) in which the first sheet 2a and the second sheet 2b are exposed. The size of the area 611 (hole 611a) is not particularly limited as long as it allows the observation of the observation target S by the observation means 4. For example, if the observation means 4 is a microscope, the size of the area 611 (hole 611a) may be set to a size corresponding to the lens diameter of the objective lens 40.

[0084] As shown in FIG. 4, when the first sheet 2a and the second sheet 2b pass through the space of the member 610, the movement of the sheets is restricted by the inner wall surface of the member 610. At this time, by arranging the range 611 (hole 611a) of the fixing jig 61 so that it is within the observation field of the observation means 4, the first sheet 2a and the second sheet 2b can be reliably aligned. In other words, it becomes easy to ensure that the observation target S is within the observation field of the observation means 4. In addition, the spatial height of the member 610 (the length in the Z direction in FIG. 4) defines the distance between the first sheet 2a and the second sheet 2b. Therefore, by providing the fixing jig 61, it becomes possible to easily adjust the distance and align the first sheet 2a and the second sheet 2b.

[0085] When the observation means 4 is a microscope, the member 610 preferably has a structure that allows it to be placed and fixed on the microscope stage. For example, the member 610 may be provided with a locking means (screw, clip, etc.) for locking the member 610 to the microscope stage, or with an adhesive means (adhesive, adhesive tape, etc.) for bonding the member 610 to the microscope stage. Alternatively, the member 610 may be provided with a structure that allows it to fit into the microscope stage. Furthermore, the member 610 may be provided with a structure that allows it to utilize a slide glass fixing member provided on the microscope stage. This allows for easy alignment of the first sheet 2a and the second sheet 2b with respect to the observation means 4. Here, the object to which the member 610 of the fixing jig 61 is fixed is not limited to the stage of the microscope, but other objects to which the member 610 can be fixed include the microscope body and the table on which the microscope is installed (such as a vibration isolation table). Furthermore, when the member 610 is arranged and fixed to the stage of the microscope, it is not limited to being arranged and fixed directly on the stage of the microscope. For example, an auxiliary member may be provided to connect the stage of the microscope and the member 610, and the member 610 may be fixed to the stage of the microscope via the auxiliary member.

[0086] Furthermore, the configuration of the member 610 and the area 611 in the fixing jig 61 is not limited to that shown in FIG. For example, the member 610 may have a rectangular parallelepiped shape with some or both sides open, which makes it easier to attach the first sheet 2a and the second sheet 2b. Furthermore, in order to increase the accuracy of the alignment of the first sheet 2a and the second sheet 2b by the member 610, a structure 612 for more reliably restricting the movement of the sheets may be provided in the member 610. This makes it possible to more reliably adjust the distance and alignment of the first sheet 2a and the second sheet 2b. Furthermore, the member 610 of the fixing jig 61 may have a structure that allows parts to be separated and may be made up of multiple structures such as members 610a and 610b. This makes it possible to easily attach the first sheet 2a and the second sheet 2b to the sheet arrangement adjustment mechanism 6 before performing the observation process.

[0087] An example of the structure of the support part 62 is one that consists of a support member 620 that supplies the first sheet 2a and / or the second sheet 2b toward the fixing jig 61 while applying an appropriate tension to the first sheet 2a and / or the second sheet 2b, as shown in Figure 4. The support member 620 has a contact portion 620a that comes into contact with the first sheet 2a and / or the second sheet 2b supplied from the unwinding portion 50 of the sheet moving mechanism 5, and as the sheets move through this contact portion 620a, an appropriate tension is applied to the first sheet 2a and / or the second sheet 2b, making it possible to suppress distortion or bending of the sheets. In addition, in Figure 4, the support part 62 is shown to be provided on the first sheet 2a side, but this is not limited to this, and it may be provided on the second sheet 2b side, or it may be provided on both the first sheet 2a side and the second sheet 2b side. Furthermore, there are no particular limitations on the locations and number of support parts 62. For example, as shown in Fig. 4, they may be provided on the upstream side, downstream side, or both the upstream and downstream sides of the objective lens 40 in the observation means 4 in the sheet movement direction. In this case, a plurality of support parts 62 may be provided consecutively on the upstream side and / or downstream side.

[0088] The shapes of the support member 620 and the contact portion 620a are not particularly limited. For example, as shown in Fig. 4, the support member 620 may be shaped as a rectangular parallelepiped, and the contact portion 620a may have a rectangular planar shape. Other examples include a support member 620 having a columnar shape with a polygonal or circular cross section (XZ plane in Fig. 4), in which the contact portion 620a has a linear shape formed by contact between the vertices of the polygon and the sheet, a planar shape formed by contact between the polygonal surface and the sheet, or an arc shape formed by contact between the circular surface and the sheet. Furthermore, the support member 620 may be a fixed rectangular parallelepiped (columnar body) as shown in FIG. 4, or may be a cylindrical body that is driven to rotate, such as a roller. When the support members 620 are provided on the first sheet 2a side and the second sheet 2b side, the shapes of the support members 620 may be the same or different. When the support members 620 are provided on the upstream side and the downstream side in the sheet movement direction, the shapes of the support members 620 may be the same or different.

[0089] The seat arrangement adjustment mechanism 6 in this embodiment may be configured such that the fixing jig 61 and the support portion 62 are integrated together. This makes it possible to reduce the number of components of the seat arrangement adjustment mechanism 6.

[0090] In the observation system 1B of this embodiment, the provision of the sheet arrangement adjustment mechanism 6 makes it easy to adjust the distance and alignment between the first sheet 2a and the second sheet 2b, and in combination with the tension control means 53, it is possible to suppress distortion or bending of the sheet under the observation means 4, securely fix the observation object S, and improve the observation accuracy. This makes it possible to further improve the efficiency of the work related to sample preparation and the work related to observation of the observation object S.

[0091] The above-described embodiment shows an example of an observation system. The observation system according to the present invention is not limited to the above-described embodiment, and the observation system according to the above-described embodiment may be modified within the scope of the gist of the claims.

[0092] For example, the observation system in this embodiment may be provided with a recovery means for recovering the observation object S after observation by the observation means 4. Note that the recovery means is not particularly limited as long as it can recover the observation object S. An example of the recovery means is a means for removing the observation object S fixed between the first sheet 2a and the second sheet 2b after peeling them off. Another example of the recovery means is a means for cutting off the sheet with the observation object S fixed between the first sheet 2a and the second sheet 2b. By collecting the observation object S after observation, it becomes possible to preserve the observation object S as a specimen along with the observation data. In particular, it becomes possible to collect observation objects S that are rare or highly valuable as specimens, such as living organisms. Furthermore, the collected observation object S can be used as a sample to supplement a database of observation data, or the observation object S itself can be reused.

[0093] Furthermore, for the supply and recovery of sheets in the observation system of this embodiment, a sheet supply and recovery mechanism other than the unwinding rolls 50a, 50b as the unwinding section 50 that unwinds and supplies the first sheet 2a and the second sheet 2b, and the winding rolls 51a, 51b as the winding section 51 that winds and recovers the first sheet 2a and the second sheet 2b, may be provided. An example of such a sheet supply / recovery mechanism is a cartridge type in which the functions related to sheet supply and recovery are housed in a casing. By using a cartridge type mechanism for sheet supply / recovery, it becomes easy to replace and store the sheets. Furthermore, since the sheets are inside the casing, it becomes easy to prevent foreign matter from getting into or adhering to the sheets before supply or after recovery during observation or sheet movement. [Industrial Applicability]

[0094] The observation system of the present invention can be used for observing various observation objects, and is particularly suitable for use in observing living organisms as observation objects. [Explanation of symbols]

[0095] 1A, 1B Observation system, 2a First sheet, 2b Second sheet, 3 Arrangement means, 30 Dispensing section, 31 Supply section, 31a Storage section, 31b Supply line, 31c Pump, 32 Control mechanism, 4 Observation means, 40 Objective lens, 5 Sheet moving mechanism, 50 Unwinding section, 50a, 50b Unwinding roll, 51 Winding section, 51a, 51b Winding roll, 52 Speed ​​adjustment function, 52a Rotation drive control section, 53 Tension control means, 53a Elastic body, 530a Constant force spring, 531a Plate-shaped spring body, 53b Support mechanism, 530b Support roller, 53c Connecting shaft, 53d Pressure receiving member, 54 Adjustment mechanism, 54a Pressing member, 6 Sheet arrangement adjustment mechanism, 61 Fixing jig, 610, 610a, 610b Member, 611 range, 611a hole, 62 support portion, 620 support member, 620a contact portion, C control means, C1 control portion, D1, D2 drum, S observation object

Claims

1. A first sheet; a placement means for placing an object to be observed on the first sheet; an observation means for observing the object to be observed placed on the first sheet; a seat moving mechanism that moves the first seat, the seat moving mechanism is provided with a constant force spring on the upstream side in the moving direction of the first seat, An observation system characterized in that the tension of the first sheet is kept constant.

2. An observation system as described in claim 1, comprising an up / down drive mechanism that moves a support body that supports the constant force spring in an up / down direction.

3. a second sheet that is superimposed on the first sheet after the object to be observed is placed on the first sheet; the observation means observes the object to be observed while it is fixed between the first sheet and the second sheet, The observation system described in claim 1 or 2, characterized in that the sheet movement mechanism has a constant force spring provided upstream in the movement direction of the first sheet and / or the second sheet, thereby maintaining constant tension in the first sheet and / or the second sheet.

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