Exosome separation and concentration method using marine animal source tissue as raw material

By employing freeze-drying, pulverization, combined enzymatic hydrolysis, and ultrasonic treatment, the problems of long separation time and low purity in existing technologies for exosomes have been solved. This enables rapid and efficient separation and concentration of exosomes from marine animal tissues, improving the efficiency of research and application.

CN121825849APending Publication Date: 2026-04-10OCEAN UNIV OF CHINA +1
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Patent Information

Application Number
CN202610070972.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-20
Publication Date
2026-04-10

AI Technical Summary

Technical Problem

Existing exosome separation techniques are time-consuming, costly, and have low purity, making it difficult to effectively remove impurities from marine animal-derived tissues, thus affecting research and application.

Method used

The process employs freeze-drying pulverization and combined enzymatic hydrolysis, along with ultrasonic oscillation. The tissue is broken down into small particles through freeze-drying pulverization, and exosomes are rapidly separated using the synergistic action of papain and trypsin. Impurities are removed through multi-stage filtration and ultrasonic treatment to ensure the purity and concentration of exosomes.

Benefits of technology

It enables rapid and high-purity separation and concentration of exosomes, reduces operating costs, improves the recovery rate and purity of exosomes, and simplifies the operation process.

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Abstract

The invention relates to the technical field of bioengineering, in particular to an exosome separation and concentration method with marine animal source tissue as a raw material. Comprising the following steps: freeze-drying and crushing a marine animal source tissue sample; performing sample enzymolysis; and removing impurities and concentrating. According to the method disclosed by the invention, the exosome in the cells is fully dissociated through freeze-dried powder crushing and composite enzymolysis processes, and the filtering speed of the sample is accelerated by virtue of ultrasonic oscillation, so that the rapid and high-purity separation and concentration of the exosome in the marine animal source tissue are realized.
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