Laser scanning imaging method, system, storage medium, and computer program

Abstract

A laser scanning imaging method, system, storage medium, and computer program, the laser scanning imaging method includes a step (S101) of determining parameter information on an effective area including an imaging area that satisfies a preset condition, a step (S102) of receiving a galvanometer mirror scanning synchronization signal generated from a driving unit, a step (S103) of generating a clock signal based on the galvanometer mirror scanning synchronization signal and the parameter information on the effective area, and a step (S104) of sampling a fluorescent signal received by the galvanometer mirror scanning according to the clock signal to obtain fluorescent image information of the effective area. This method realizes that the generated clock signal can be flexibly changed according to a change in the range of the effective area, whereby a fluorescent image of the effective area is obtained, and a scanning imaging result that satisfies the requirement is presented to a user.

Description

[Technical Field]

[0001] The present application relates to laser scanning optical imaging technology, and in particular to a laser scanning imaging method, system, storage medium and computer program. [Background technology]

[0002] In modern lifestyles, cancer-related cases are increasing. Laser scanning imaging systems can provide real-time tissue information with subcellular resolution, which is expected to have great prospects for future applications in early detection, screening, and diagnosis of cancer. Laser scanning imaging systems include major components such as a laser device, a laser scanner, an objective lens, a small hole, and an optical sensor.

[0003] During laser scanning imaging, the laser scanning imaging system is configured such that a laser device emits a laser beam, the laser scanning device controls the deflection of the laser beam to perform scanning, the objective lens focuses the laser beam on biological tissue, and the fluorescent information generated by the biological tissue after irradiation passes through the objective lens, the laser scanning device, and the small hole and reaches the optical sensor where it is received, thereby forming fluorescent information of the irradiated area.

[0004] A resonant galvanometer mirror is used as a fast mirror in a laser scanning device and typically moves nonlinearly. As the resonant galvanometer mirror moves, the laser scanning device outputs a galvanometer mirror scanning synchronization signal, which is a square wave that indicates the change in the galvanometer mirror movement direction. A hardware clock board typically receives the galvanometer mirror scanning synchronization signal and generates a clock signal for sampling the fluorescence signal.

[0005] Conventionally, clock signals are generated in the form of hardware clock boards. However, the clock signal is generated only at one fixed portion where the movement of the resonant galvanometer mirror is relatively linear. This means that the imaging range of the corresponding resonant galvanometer mirror is relatively small and the imaging position is fixed. In addition, the clock signal generated in this form has fixed characteristics and cannot be flexibly changed in response to changes in the scanning range. As a result, the area of ​​the obtained fluorescent image is relatively small and fixed, and even if the imaging area is subsequently changed by enlargement processing or the like, the actual resolution of the fluorescent image cannot be improved. Summary of the Invention [Problem to be solved by the invention]

[0006] In response to the above technical problems existing in the prior art, the present application provides a laser scanning imaging method, system, storage medium, and computer program that allows the generated clock signal to be flexibly changed in response to changes in the range of the effective area, thereby presenting the user with scanning imaging results that meet their requirements. [Means for solving the problem]

[0007] An embodiment of the present application provides a laser scanning imaging method, the method including the steps of: determining parameter information for an effective area including an imaging area that satisfies a preset condition; receiving a galvanometer mirror scanning synchronization signal generated from a driving unit; generating a clock signal based on the galvanometer mirror scanning synchronization signal and the parameter information for the effective area; and sampling a fluorescence signal received by galvanometer mirror scanning according to the clock signal to obtain fluorescence image information of the effective area.

[0008] An embodiment of the present application further provides a laser scanning imaging system, the laser scanning imaging system including a galvanometer mirror and a processor, the processor being connected to the galvanometer mirror and being used to perform the above laser scanning imaging method.

[0009] An embodiment of the present application further provides a storage medium having a computer program stored thereon, which, when executed by a processor, implements the laser scanning imaging method according to any of the embodiments of the present application.

[0010] An embodiment of the present application further provides a computer program, which, when executed by a processor, implements the laser scanning imaging method according to any of the embodiments of the present application. [Effects of the Invention]

[0011] Compared to conventional technologies, the present application generates a clock signal based on the received galvanometer mirror scanning synchronization signal and parameter information related to the effective area, samples the fluorescence signal received by the galvanometer mirror scanning according to the clock signal, and obtains fluorescence image information of the effective area, thereby realizing that the generated clock signal can flexibly change according to changes in the range of the effective area, and a fluorescence image of the effective area can be obtained, and the required scanning imaging result can be presented to the user. [Brief explanation of the drawings]

[0012] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar components in different drawings. The same reference numerals followed by an alphabetical or different alphabetical characters may represent different instantiations of similar components. The drawings illustrate various embodiments generally by way of example, not limitation, and are used in conjunction with the specification and claims to explain the described embodiments. Where appropriate, the same or similar parts are designated by the same reference numerals in all drawings. Such embodiments are illustrative and are not intended as exhaustive or exclusive embodiments of the present apparatus or methods. [Figure 1] 1 is a flowchart of a laser scanning imaging method according to an embodiment of the present application. [Figure 2] 4 is another flowchart of the laser scanning imaging method according to an embodiment of the present application. [Figure 3] 4 is another flowchart of a laser scanning imaging method according to an embodiment of the present application. [Figure 4] 4 is another flowchart of a laser scanning imaging method according to an embodiment of the present application. [Figure 5] FIG. 1 is a schematic diagram of the hardware structure of an electronic device provided by an embodiment of the present application. DETAILED DESCRIPTION OF THE INVENTION

[0013] Various aspects and features of the present application are described herein with reference to the accompanying drawings.

[0014] It will be understood that various modifications can be made to the embodiments of the present application. Therefore, the above description should not be considered limiting, but rather an example of an embodiment. Those skilled in the art will envision other modifications within the scope and spirit of the present application.

[0015] The drawings incorporated in and constituting a part of this specification illustrate embodiments of the present application and, together with the general description of the present application given above and the detailed description of the embodiments given below, serve to explain the principles of the present application.

[0016] These and other features of the present application will become apparent from the following description of selective forms of embodiment given as non-limiting examples, with reference to the accompanying drawings, in which:

[0017] Although the present application has been described with reference to a few specific examples, it should also be understood that those skilled in the art will no doubt be able to implement many other equivalent forms of the present application.

[0018] These and other aspects, features, and advantages of the present application will become more apparent in light of the following detailed description, taken in conjunction with the accompanying drawings.

[0019] Specific embodiments of the present application will be described below with reference to the drawings. However, it should be understood that the embodiments of the present application are merely examples of the present application and can be implemented in various forms. Well-known and / or overlapping functions and structures are not described in detail to avoid obscuring the present application with unnecessary or redundant details. Therefore, the specific structural and functional details described herein are not intended to be limiting, but are used only as a basis for the claims and as a representative basis to teach those skilled in the art how to use the present application in various ways with substantially any appropriate detailed structure.

[0020] The terms "in one embodiment," "in another embodiment," "in another embodiment," or "in other embodiments" may be used herein, any of which may refer to one or more of the same or different embodiments of the present application.

[0021] An embodiment of the present application provides a laser scanning imaging method, which can be applied to a laser scanning imaging system. As shown in Figure 1, the laser scanning imaging method includes steps S101 to S104.

[0022] In step S101, parameter information relating to the effective area is determined, and the effective area includes an imaging area that satisfies a preset condition.

[0023] In step S102, the galvanometer mirror scanning synchronization signal generated by the driving unit is received.

[0024] In step S103, a clock signal is generated based on the galvanometer mirror scanning synchronization signal and parameter information relating to the effective area.

[0025] In step S104, the fluorescent light signals received by the galvanometer mirror scanning are sampled in accordance with the clock signal, and fluorescent light image information of the effective area is obtained.

[0026] Specifically, the galvanometer mirror may be a linear galvanometer mirror, a nonlinear galvanometer mirror, or a combination of a linear galvanometer mirror and a nonlinear galvanometer mirror. When the galvanometer mirror is either a linear galvanometer mirror or a nonlinear galvanometer mirror, one-dimensional fluorescence image information of the effective area is obtained by steps S101 to S104. When the galvanometer mirror is equipped with a nonlinear galvanometer mirror in addition to a linear galvanometer mirror, two-dimensional fluorescence image information of the effective area is obtained by steps S101 to S104. Here, the nonlinear galvanometer mirror is the resonant galvanometer mirror described above.

[0027] Specifically, a user can set an imaging area that satisfies a predetermined condition, i.e., an area that requires fluorescent imaging. The effective area can be understood as an imaging area that needs to be displayed to the user. The effective area may be the entire scanning area of ​​the laser scanning imaging system or a portion of the entire scanning area, and is specifically determined according to the user's needs.

[0028] Specifically, the driving unit may be a nonlinear driving unit. The nonlinear galvanometer mirror in the laser scanning imaging system self-oscillates and moves nonlinearly after being energized. The nonlinear driving unit generates a galvanometer mirror scanning synchronization signal for the nonlinear galvanometer mirror. The galvanometer mirror scanning synchronization signal is synchronized with the movement of the nonlinear galvanometer mirror, and the specific form of the galvanometer mirror scanning synchronization signal may be a square wave signal. The laser scanning imaging system receives the galvanometer mirror scanning synchronization signal and generates a clock signal based on the galvanometer mirror scanning synchronization signal and parameter information related to the effective area, thereby enabling the generated clock signal to flexibly change with changes in the range of the effective area to be imaged, thereby satisfying user imaging requirements for various areas and various resolutions. This avoids the need for a hardware clock board to receive the galvanometer mirror scanning synchronization signal in the prior art, which can only generate a fixed clock signal for sampling the fluorescence signal, thereby only being able to image a fixed area.

[0029] Using the solution of the present application, the user can set the area where imaging is performed (i.e., the effective area) according to the imaging request, thereby enabling the generated clock signal to flexibly change according to changes in the range of the effective area, thereby obtaining a fluorescent image of the effective area by sampling based on the clock signal, and presenting the user with the scanning imaging results that meet the request.

[0030] In some embodiments, the parameter information includes area range information for the validity area.

[0031] In some embodiments, the region range information includes at least a start position, an end position, and a pixel count.

[0032] Specifically, the start position, end position, and number of pixels of the effective area can be determined according to the imaging area that needs to be displayed to the user, and the imaging area displayed to the user is the area that the user requests to capture. The start position, end position, and number of pixels are parameter information related to the effective area. Here, the start position and end position may be expressed by coordinates (one-dimensional, two-dimensional, or three-dimensional) or by the galvanometer mirror deflection angle.

[0033] Alternatively, for example, if a user needs to enlarge the scanning and imaging result, the user may increase the number of pixels in the effective area without changing the set values ​​of the start position and the end position to obtain an enlarged display of the scanning and imaging result, or may keep the number of pixels the same and shorten the distance between the start position and the end position to obtain an enlarged display of the scanning and imaging result. Alternatively, for example, if a user needs to reduce the scanning and imaging result, the user may reduce the number of pixels in the effective area without changing the set values ​​of the start position and the end position to obtain a reduced display of the scanning and imaging result, or may keep the number of pixels the same and increase the distance between the start position and the end position to obtain an enlarged display of the scanning and imaging result. The number of pixels refers to the number of pixel points, i.e., the number of pixel points.

[0034] The following description will be given taking as an example a case where the galvanometer mirror is a nonlinear galvanometer mirror.

[0035] In some embodiments, as shown in FIG. 2, step S103 of generating a clock signal based on the galvanometer mirror scanning synchronization signal and the parameter information related to the effective area specifically includes steps S201 to S204.

[0036] In step S201, galvanometer mirror movement parameter information corresponding to each pixel point within the effective area is obtained according to the area range information.

[0037] In step S202, image scanning parameter information corresponding to each pixel point within the effective area is obtained according to the galvanometer mirror movement parameter information corresponding to each pixel point within the effective area.

[0038] In step S203, scan time information corresponding to the effective area is determined according to the galvanometer mirror scan synchronization signal and area range information.

[0039] In step S204, the image scanning parameter information and the scanning time information are quantized to generate a clock signal.

[0040] In some embodiments, the galvanometer mirror movement parameter information includes at least one of information on the movement speed of the galvanometer mirror, information on the movement acceleration of the galvanometer mirror, information on the movement frequency of the galvanometer mirror, information on the deflection angle of the galvanometer mirror, information on the spatial position of the edge of the galvanometer mirror, and information on the spatial position of the galvanometer mirror lens. Here, the movement frequency information of the galvanometer mirror can be understood as information on the oscillation frequency of the galvanometer mirror.

[0041] In some embodiments, the image scanning parameter information includes at least one of sampling position information corresponding to each pixel point, sampling time information corresponding to each pixel point, and sampling duration information corresponding to each pixel point.

[0042] In some embodiments, the scan time information includes at least any two scan times corresponding to the valid area. Optionally, when the scan time information includes any two scan times corresponding to the valid area, the any two scan times corresponding to the valid area may be a start time and an end time, a start time and any scan time other than the start time, or an end time and any scan time other than the end time.

[0043] In some embodiments, the area range information includes at least a start position, an end position, and the number of pixel points. Hereinafter, a specific method for generating a clock signal will be described with reference to FIG. 3 , taking as an example a case where the galvanometer mirror movement parameter information includes galvanometer mirror movement speed information and the image scanning parameter information includes sampling time information corresponding to each pixel point. The method includes the following steps S301 to S304.

[0044] In step S301, the galvanometer mirror movement speed information corresponding to each pixel point within the effective area is obtained according to the start position, end position, and number of pixels.

[0045] In step S302, sampling time information corresponding to each pixel point within the effective area is obtained in accordance with the galvanometer mirror movement speed information corresponding to each pixel point within the effective area.

[0046] In step S303, scan time information corresponding to the effective area is determined according to the galvanometer mirror scan synchronization signal and area range information.

[0047] Specifically, in this embodiment, the scanning time information includes a start time and an end time.

[0048] In step S304, the sampling time information and the scanning time information are quantized to generate a clock signal, that is, the sampling time information and the start time and end time are quantized to generate a clock signal.

[0049] Specifically, when the start position and the end position are expressed in the form of one-dimensional coordinates, the corresponding coordinate values ​​are

number

number

number

[0050] Specifically, the start and end positions are determined by the deflection angle of the galvanometer mirror.

number

[0051] Optionally, the moving speed of the nonlinear galvanometer mirror corresponding to each pixel point in the effective area is set as follows:

number

number

[0052] In some embodiments, the area range information includes at least a start position, an end position, and the number of pixel points. Hereinafter, with reference to FIG. 4 , another specific method for generating a clock signal will be described, taking as an example a case where the galvanometer mirror movement parameter information includes galvanometer mirror deflection angle information and the image scanning parameter information includes sampling time information corresponding to each pixel point. The method includes the following steps S401 to S404.

[0053] In step S401, the galvanometer mirror deflection angle information corresponding to each pixel point within the effective area is obtained according to the start position, end position, number of pixels, and galvanometer mirror distance parameters.

[0054] In step S402, sampling time information corresponding to each pixel point within the effective area is obtained according to the galvanometer mirror deflection angle information corresponding to each pixel point within the effective area.

[0055] In step S403, scan time information corresponding to the effective area is determined according to the galvanometer mirror scan synchronization signal and area range information.

[0056] Specifically, in this embodiment, the scanning time information includes a start time and an end time.

[0057] In step S404, the sampling time information and the scanning time information are quantized to generate a clock signal, that is, the sampling time information and the start time and end time are quantized to generate a clock signal.

[0058] Specifically, according to the start position, end position, and number of pixels, the relative coordinates of each pixel point in the effective area relative to the start position are:

number

number

number

number

[0059] The above specific method for generating a clock signal is illustrative rather than limiting, and any method for generating a clock signal using the above-mentioned galvanometer mirror scanning synchronization signal and parameter information related to the effective area falls within the scope of protection of the present application.

[0060] In some embodiments, the area range information includes at least a start position and an end position, and wherein step S203 of determining time scanning information corresponding to the effective area according to the galvanometer mirror scanning synchronization signal and the area range information includes: determining a start time corresponding to an effective area according to a start position and a period of a galvanometer mirror scanning synchronization signal; and determining an end time corresponding to the effective area according to the end position and the period of the galvanometer mirror scanning synchronization signal.

[0061] Specifically, the start time and end time corresponding to the valid area are

number

number

[0062] In some embodiments, the galvanometer mirror includes a first galvanometer mirror and a second galvanometer mirror, the first galvanometer mirror and the second galvanometer mirror being disposed perpendicular to each other, and the laser scanning method comprises: determining a control signal for a second galvanometer mirror according to the galvanometer mirror scanning synchronization signal and the effective area; Controlling and driving a first galvanometer mirror to move along a first direction; and controlling the second galvanometer mirror to move along the second direction according to the control signal of the second galvanometer mirror.

[0063] Specifically, the drive unit may be a nonlinear drive unit. The first galvanometer mirror may be the nonlinear galvanometer mirror described above, and the second galvanometer mirror may be the linear galvanometer mirror described above. The first direction may be perpendicular to the second direction. For example, the first direction may be the X-axis direction in a Cartesian coordinate system, and the second direction may be the Y-axis direction in a Cartesian coordinate system. That is, the nonlinear galvanometer mirror may move along the X-axis direction, and the linear galvanometer mirror may move along the Y-axis direction. Optionally, the X-axis direction may be horizontal, and the Y-axis direction may be vertical.

[0064] The process for obtaining fluorescence image information in this embodiment will be described using an example in which the first direction is the horizontal direction, the second direction is the vertical direction, and the effective area is a portion of the entire scanning area. Starting from the scanning start position of the entire scanning area, the nonlinear galvanometer mirror is first driven to move horizontally from the scanning start position. When the nonlinear galvanometer mirror moves to the maximum scanning position along the horizontal direction, the linear galvanometer mirror is driven to move vertically. When the linear galvanometer mirror moves vertically to the next position, the nonlinear galvanometer mirror is driven to move horizontally. This process is repeated until the first and second galvanometer mirrors cooperate to complete movement within the entire scanning area. During the above movement, the two galvanometer mirrors sample the fluorescence signals within the effective area in accordance with the generated clock signal associated with the effective area, thereby obtaining two-dimensional fluorescence image information of the effective area.

[0065] Using the method of this embodiment, the two galvanometer mirrors can be moved along two mutually perpendicular directions, respectively, to finally obtain two-dimensional fluorescence image information, which further improves the accuracy of the obtained fluorescence image information and obtains image information in more dimensions, thereby ensuring the accuracy of subsequent diagnosis.

[0066] In some embodiments, determining a control signal for the second galvanometer mirror according to the galvanometer mirror scanning synchronization signal and the effective area includes determining a control signal for the second galvanometer mirror according to parameter information relating to the galvanometer mirror scanning synchronization signal and the effective area.

[0067] Specifically, the parameter information can include the start position, end position, and number of pixels, all of which are related to the effective area, and the movement status of the linear galvanometer mirror can be determined according to this information, thereby realizing control over the movement of the linear galvanometer mirror, and finally obtaining a fluorescent image of the effective area.

[0068] An embodiment of the present application further provides a laser scanning imaging system, the system comprising a galvanometer mirror and a processor connected to the galvanometer mirror, wherein the processor is used to perform the laser scanning imaging method provided by any one of the above embodiments.

[0069] FIG. 5 is a schematic diagram of the hardware structure of a laser scanning imaging system that implements the laser scanning imaging method provided by an embodiment of the present application. As shown in FIG. 5, the laser scanning imaging system includes one or more processors 510 and a memory 520. In FIG. 5, one processor 510 is shown as an example. The laser scanning imaging system that implements the laser scanning imaging method may further include an input device 530 (including a galvanometer mirror) and an output device 540.

[0070] The processor 510, memory 520, input device 530, and output device 540 may be connected by a bus or other means, and FIG. 5 shows them connected by a bus.

[0071] The memory 520, as a non-volatile computer-readable storage medium, can be used to store non-volatile software programs, non-volatile computer-executable programs and modules, such as program instructions / modules corresponding to the laser scanning imaging method in the embodiments of the present application. The processor 510 executes the non-volatile software programs, instructions and modules stored in the memory 520 to perform various functional applications and data processing of the server, thereby realizing the laser scanning imaging method in the above-mentioned embodiment of the method.

[0072] The memory 520 may include high-speed random access memory, and may also include non-volatile memory, such as at least one magnetic disk storage device, flash memory device, or other non-volatile solid-state storage device. Examples of such networks include, but are not limited to, the Internet, a corporate intranet, a local area network, a mobile communications network, and combinations thereof.

[0073] Using the method of the present application, a user can set an effective area according to imaging requirements, realize that the generated clock signal can flexibly change according to changes in the range of the effective area, and then sample based on the clock signal to obtain a fluorescent image within the effective area, and present the scanning imaging result that meets the requirements to the user.

[0074] An embodiment of the present application further provides a non-volatile storage medium having a computer program stored thereon, which, when executed by a processor, performs the steps of the laser scanning imaging method according to any one of the embodiments of the present application. Those skilled in the art will understand that all or part of the steps included in the method of the above embodiment can be accomplished by instructing associated hardware with a program that can be stored on a medium, and when the program is executed, one or a combination of the steps in the method embodiment is performed. The medium may be a storage medium or a signal medium. The storage medium may be, but is not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination thereof. Specific examples include a portable computer magnetic disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM) (or flash memory), an optical fiber, a portable optical disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any combination thereof. The signal medium may include, but is not limited to, an electrical signal medium, an optical signal medium, a radio wave (electromagnetic wave) medium, or any combination thereof.

[0075] Furthermore, each functional unit in each embodiment of the present application may be integrated into one processing module, or each unit may exist physically separately, or two or more units may be integrated into one module. The integrated module may be implemented in the form of hardware or in the form of a software functional module. When the integrated module is implemented in the form of a software functional module and sold or used as an independent product, it may be stored in a computer-readable storage medium.

[0076] The storage medium mentioned above may be a read-only memory, a magnetic disk, an optical disk, etc.

[0077] Note that each unit in each embodiment of the present application may be implemented as computer-executable instructions stored on a memory, which, when executed by a processor, perform corresponding steps, and may be implemented as hardware with appropriate logical computing capabilities, or as a combination of software and hardware (firmware). In some embodiments, the processor may be implemented as any of a field programmable gate array (FPGA), an application specific integrated circuit (ASIC), a digital signal processor (DSP) chip, a system on a chip (SOC), a microprocessor (MPU) (including, but not limited to, Cortex), etc.

[0078] It should be noted that, although each component of the system of the present application is logically divided according to the function to be realized, the present application is not limited to this, and each component may be further divided or combined as necessary; for example, some of the components may be combined as a single component, or some of the components may be further selectively divided into sub-components.

[0079] Each component of the present specification may be implemented in hardware, software modules running on one or more processors, or a combination of these. Those skilled in the art should understand that some or all of the functions of some or all of the components of the system according to the present specification may be implemented using a microprocessor or a digital signal processor (DSP) in practice. The present specification may be realized as a device or apparatus program (e.g., a computer program or computer program product) for executing some or all of the methods described herein. Such a program implementing the present specification may be stored on a computer-readable medium or may have the form of one or more signals. Such signals may be downloaded from an Internet website, provided on a carrier signal, or provided in any other form. Furthermore, the present specification may be implemented by hardware comprising several different components and a suitably programmed computer. In a unit claim enumerating several devices, several of these devices may be embodied by the same hardware element. The use of terms such as first, second, and third does not imply any ordering. These terms may be interpreted as names.

[0080] Furthermore, although exemplary embodiments are described herein, the scope includes any and all embodiments based on this application, including equivalent elements, modifications, omissions, combinations (e.g., cross-sectional schemes of various embodiments), edits, or variations. Claim elements should be construed broadly based on the language used in the claims and not limited to the examples described in the specification or in practicing this application, which examples should be construed as non-exclusive. Accordingly, the specification and examples are intended to be considered merely as examples, with the actual scope and spirit being indicated by the following claims and the full scope of equivalents thereto.

[0081] The above description is intended to be illustrative, not limiting. For example, the above examples (or one or more aspects thereof) can be used in combination with each other. Other examples may be used, for example, as one of ordinary skill in the art may read the above description. Furthermore, in the specific embodiments described above, various features may be grouped together to simplify the application. This should not be interpreted as an intention that non-claimed described features are required in any claim. Conversely, the subject matter of the application may include less than all features of a particular described embodiment. Thus, the following claims are incorporated herein by way of example or example into the specific embodiments, with each claim considered to stand alone as a separate example, and these examples can be combined with each other in various combinations or permutations. The scope of the application should be determined with reference to the appended claims, along with the full range of equivalent forms to which those claims are entitled.

[0082] The above embodiments are illustrative examples of the present application and are not intended to limit the present application, and the scope of the present application is limited by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and these modifications or equivalent substitutions shall be deemed to be within the protection scope of the present application.

[0083] This application is filed with the China Patent Office on July 27, 2021, under application number 202 11 0852227.9, which claims priority from Chinese patent application entitled "Laser scanning imaging method, system and storage medium," the entire contents of which are incorporated herein by reference.

Claims

1. 1. A laser scanning imaging method, comprising: determining parameter information relating to an effective area including an imaging area that satisfies a preset condition; receiving a galvanometer mirror scanning synchronization signal generated from a driving unit; generating a clock signal based on the galvanometer mirror scanning synchronization signal and parameter information related to the effective area; sampling the received fluorescence signal by scanning the galvanometer mirror in accordance with the clock signal to obtain fluorescence image information of the effective area; the parameter information includes area range information of the effective area, the galvanometer mirror scanning synchronization signal is a signal synchronized with the movement of the galvanometer mirror, generating a clock signal based on the galvanometer mirror scanning synchronization signal and parameter information related to the effective area, determining galvanometer mirror movement parameter information corresponding to each pixel point within the effective area according to the area range information; determining image scanning parameter information corresponding to each pixel point within the effective area according to galvanometer mirror movement parameter information corresponding to each pixel point within the effective area; determining scan time information corresponding to the effective area according to the galvanometer mirror scan synchronization signal and the area range information; performing a quantization process on the image scanning parameter information and the scanning time information to generate the clock signal; the area range information includes at least a start position, an end position, and a number of pixels; the step of determining galvanometer mirror movement parameter information corresponding to each pixel point within the effective area in accordance with the area range information includes the step of determining galvanometer mirror deflection angle information corresponding to each pixel point within the effective area in accordance with the start position, the end position, the number of pixels, and a galvanometer mirror distance parameter; a step of determining image scanning parameter information corresponding to each pixel point in the effective area in accordance with galvanometer mirror movement parameter information corresponding to each pixel point in the effective area, comprising the step of determining sampling time information corresponding to each pixel point in the effective area in accordance with galvanometer mirror deflection angle information corresponding to each pixel point in the effective area.

2. 2. The laser scanning imaging method according to claim 1, wherein the galvanometer mirror movement parameter information includes at least one of information on a movement speed of the galvanometer mirror, information on an acceleration of the galvanometer mirror, information on a deflection angle of the galvanometer mirror, and information on a movement frequency of the galvanometer mirror.

3. 2. The laser scanning imaging method according to claim 1, wherein the image scanning parameter information includes at least one of sampling position information corresponding to each of the pixel points, sampling time information corresponding to each of the pixel points, and sampling duration information corresponding to each of the pixel points.

4. the area range information includes at least a start position and an end position, and the step of determining scan time information corresponding to the effective area according to the galvanometer mirror scan synchronization signal and the area range information includes: determining a start time corresponding to the effective area according to the start position and a period of the galvanometer mirror scanning synchronization signal; 2. The laser scanning imaging method according to claim 1, further comprising the step of: determining an end time corresponding to the effective area according to the end position and a period of the galvanometer mirror scan synchronization signal.

5. the galvanometer mirror includes a first galvanometer mirror and a second galvanometer mirror, the first galvanometer mirror and the second galvanometer mirror having moving directions perpendicular to each other, and the laser scanning imaging method includes: determining a control signal for a second galvanometer mirror according to the galvanometer mirror scanning synchronization signal and the effective area; controlling the drive unit to drive movement of the first galvanometer mirror along a first direction; 2. The laser scanning imaging method of claim 1, further comprising the step of: controlling the second galvanometer mirror to move along a second direction according to a control signal of the second galvanometer mirror.

6. determining a control signal for a second galvanometer mirror according to the galvanometer mirror scan synchronization signal and the effective area, 6. The laser scanning imaging method according to claim 5, further comprising the step of determining a control signal for the second galvanometer mirror according to the galvanometer mirror scanning synchronization signal and parameter information relating to the effective area.

7. 1. A laser scanning imaging system, comprising: Galvanometer mirror and a processor connected to the galvanometer mirror, the processor being used to execute the laser scanning imaging method according to any one of claims 1 to 6.

8. A storage medium having a computer program stored thereon, the computer program causing a laser scanning imaging method according to any one of claims 1 to 6 to be performed when the computer program is executed by a processor.

9. A computer program, characterized in that when the computer program is executed by a processor, the method for laser scanning imaging according to any one of claims 1 to 6 is performed.

Images