TROP2-binding nanobodies and uses thereof

Abstract

A heavy chain-only antibody that specifically binds to human TROP2 or an antigen-binding portion thereof. Also provided are nucleic acid molecules encoding the antibody or antigen-binding portion thereof, expression vectors, host cells, and methods for expressing the antibody or antigen-binding portion thereof. The present disclosure further provides bispecific molecules, immunoconjugates and pharmaceutical compositions comprising the antibody or antigen-binding portion thereof, and methods of treatment using the anti-TROP2 antibody or antigen-binding portion thereof of the present disclosure.

Description

[Technical Field]

[0001] Related Applications and Incorporation by Reference This application claims priority to Chinese Application No. 202011209105.X, filed November 3, 2020, and U.S. Provisional Patent Application Serial No. 63 / 178,741, filed April 23, 2021.

[0002] The aforementioned applications, all documents cited therein or cited during prosecution thereof ("appln cited documents"), and all documents cited or referenced herein (including, but not limited to, all literature documents, patents, and published patent applications cited herein) ("herein cited documents"), together with manufacturer's instructions, descriptions, product specifications, product sheets for any products described herein or in the documents incorporated by reference, are hereby incorporated by reference and may be employed in the practice of this invention. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference. Any Genbank sequences referred to in this disclosure are incorporated by reference as the Genbank sequence as of the earliest effective filing date of this disclosure.

[0003] FIELD OF THE INVENTION The present disclosure generally relates to isolated monoclonal heavy chain-only antibodies, or antigen-binding portions thereof, that bind to human TROP2 with high affinity and functionality. Nucleic acid molecules encoding the antibodies or antigen-binding portions thereof, expression vectors, host cells, and methods for expressing the antibodies or antigen-binding portions thereof are also provided. The present disclosure further provides bispecific molecules, immunoconjugates, chimeric antigen receptors, oncolytic viruses, and pharmaceutical compositions that can include the antibodies or antigen-binding portions thereof, as well as methods of treatment using the anti-TROP2 antibodies or antigen-binding portions thereof of the present disclosure.

[0004] Background of the Invention TROP2 is a transmembrane glycoprotein also known as epithelial glycoprotein-1 (EGP-1), membrane component surface marker 1 (M1S1), tumor-associated calcium signal transducer 2 (TACSTD2), and gastrointestinal antigen 733-1 (GA733-1). The TROP2 molecule consists of a hydrophobic precursor peptide, an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The cytoplasmic tail contains a highly conserved phosphatidylinositol 4,5-bisphosphate (PIP2)-binding sequence and a serine phosphorylation site at position 303 (Zaman S et al., (2019) Targeting Trop-2 in solid tumors: future prospect. Onco Targets Ther. 12:1781-1790). TROP2 binding partners include IFG-1, claudin-1, claudin-7, cyclin D1, and PKC (Shvartsur A et al., (2015) Trop2 and its overexpression in cancers: regulation and clinical / therapeutic implications. Genes Cancer. 6(3-4):84-105).

[0005] TROP2 is expressed at low levels in normal tissues and is involved in embryonic organogenesis and fetal growth. However, elevated TROP2 expression has been confirmed in all cancer types, regardless of normal tissue TROP2 levels (Mustata RC et al., (2013) Identification of Lgr5-independent spheroid-generating progenitors of mouse fetal intestinal epithelium. Cell Reports. 5(2):421-432; Guerra E et al., (2012) mTrop1 / Epcam knockout mice develop congenital tufting enteropathy through dysegmentation of intestinal e-cadherin / β-catenin. PLoS ONE. 7(11): e49302; Trerotola M et al., (2013) Upregulation of Trop-2 quantitatively stimulates human cancer growth. Oncogene. 32(2):222-233). Studies have shown that several transcription factors on which TROP2 expression depends, such as TP63 / TP53L and Wilms' tumor 1 (WT1), are correlated with cancer development, demonstrating that TROP2 is involved in many cell signaling pathways related to tumorigenesis. For example, TROP2 signaling regulates cell self-renewal and proliferation via β-catenin signaling, thereby promoting the stem cell properties of cancer cells (Stoyanova T et al., (2012) Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via β-catenin signal. Genes Dev. 26(20):2271-2285).Overexpression of TROP2 promotes tumor invasion in cervical, ovarian, colorectal, and thyroid cancers, and knockdown of TROP2 reduces cancer cell invasion. (Guan H et al., (2017) Trop2 enhances invasion of thyroid cancer by inducing MMP2 through the ERK and JNK pathway. BMC Cancer. 17(1):486; Liu T et al., (2013) Overexpression of Trop2 predicts poor prognosis of patients with cervical cancer and promotes the proliferation and invasion of cervical cancer cells by regulating the ERK signaling pathway. PLoS One. 8(9):e75864; Wu B et al., (2017) Overexpression of Trop2 promotes proliferation and invasion of ovarian cancer cells. Exp Ther Med. 14(3):1947-1952; Zhao P et al., (2018) TNF-α promotes colon cancer cell migration and invasion by (Trop-2 upregulates Trop-2. Oncol Lett. 15(3):3820-3827). In recent years, TROP2 signaling has been shown to further regulate signals for cell migration. For example, it has been reported that TROP2 regulates β1 integrin function and promotes prostate cancer metastasis (Trerotola M et al., (2013) Trop-2 promotes prostate cancer metastasis by modulating β(1) integrin functions. Cancer Res. 73(10):3155-3167).

[0006] High expression of TROP2 has been shown to correlate with poor clinical prognosis in hilar bile duct cancer, cervical cancer, gastric cancer, and other cancers. In a meta-analysis of 2,569 patients, increased expression of TROP2 was statistically associated with poor overall survival and disease-free survival in several solid tumors (Fong D et al., (2008) High expression of Trop2 correlates with poor prognosis in pancreatic cancer. Br J Cancer. 99(8):1290-1295; Ning S et al., (2013) Trop2 correlates with microvessel density and poor prognosis in hilar cholangiocarcinoma. J Gastrointest Surg. 17(2):360-368; Liu T et al., (2013) Overexpression of Trop2 predicts poor prognosis of patients with cervical cancer and promotes the proliferation and invasion of cervical cancer cells by regulating the ERK signaling pathway. PLoS One. 8(9):e75864; Zhao W et al., (2016) Trop2 is overexpressed in gastric Cancer and predicts poor prognosis. Oncotarget.7(5):6136-6145; Zeng P et al.,(2016)Impact of Trop2 expression on prognosis in solid tumors: a systematic review and meta-analysis. Sci Rep.6:33658). The role of TROP2 as a tumor marker has also been validated in a clinical trial.

[0007] TROP2 has been an attractive therapeutic target due to its structural features and its correlation with cancer. Several anti-TROP2 antibodies have been prepared, and some have been shown to suppress breast cancer progression and induce apoptosis in xenograft mouse models (Lin H et al., (2014) A novel human Fab antibody for Trop2 inhibits breast cancer growth in vitro and in vivo. Int J Cancer. 134(5):1239-1249). However, until IKEDA et al. identified Pr1E11, which has high binding affinity and low internalization activity, none of these antibodies demonstrated therapeutic value as naked antibodies, likely due to its high internalization rate (Ikeda M et al., (2015) Pr1E11, a novel anti-TROP-2 antibody isolated by adenovirus-based antibody screening, recognizes the unique epitope. Biochem Biophys Res Commun. 458(4):877-82). Subsequent studies have shown that Pr1E11 induces potent antibody-dependent cellular cytotoxicity in vivo, which is presumably related to its high cell surface retention (Ikeda M et al., (2016) Cell Surface Antibody Retention Influences In Vivo Antitumor Activity Mediated by Antibody-dependent Cellular Cytotoxicity. Anticancer Res. 36(11):5937-5944). Currently, most TROP2-targeting therapeutics in preclinical and clinical trials are antibody-drug conjugates (ADCs), such as DS-1062a, IMMU-132, and PF-06664178, which have shown promising results in the treatment of solid tumors with limited toxicity (Zaman S et al., (2019) supra).

[0008] There is an additional need for anti-TROP2 antibodies that have low internalization activity for use as naked antibodies or high internalization activity for ADC preparation.

[0009] Citation or identification of any document in this application is not an admission that such document is available as prior art to the present invention. Summary of the Invention

[0010] The present disclosure provides isolated heavy chain-only antibodies, or antigen-binding portions thereof, that bind to TROP2 (e.g., human TROP2) and have similar, if not greater, binding affinity / capacity to human and / or monkey TROP2 and similar, if not greater, internalization activity compared to prior art anti-TROP2 antibodies such as sacituzumab (the antibody portion of IMMU-132).

[0011] The heavy chain-only antibodies or antigen-binding portions of the present disclosure can be used in a variety of applications, including detection of TROP2 protein when radiolabeled in vitro and in vivo, and treatment of TROP2-related diseases such as cancer.

[0012] Accordingly, in one aspect, the disclosure relates to an isolated monoclonal heavy chain-only antibody (e.g., a camelid, chimeric, or humanized antibody) or antigen-binding portion thereof that binds to TROP2 having a variable region that may comprise a VH CDR1 region, a VH CDR2 region, and a VH CDR3 region, wherein the VH CDR1 region, the VH CDR2 region, and the VH CDR3 region may comprise an amino acid sequence having at least 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the following: (1) SEQ ID NOs: 1, 2 (X1=D, X2=G, X3=D, X4=S) and 3 (X1=D, X2=G), respectively; (2) SEQ ID NOs: 1, 2 (X1=D, X2=G, X3=D, X4=S) and 3 (X1=E, X2=G), respectively; (3) SEQ ID NOs: 1, 2 (X1=D, X2=G, X3=D, X4=S) and 3 (X1=D, X2=A), respectively; (4) SEQ ID NOs: 1, 2 (X1=D, X2=G, X3=D, X4=S) and 3 (X1=I, X2=G), respectively; (5) SEQ ID NOs: 1, 2 (X1=E, X2=G, X3=D, X4=S) and 3 (X1=E, X2=G), respectively; (6) SEQ ID (7) SEQ ID NOs: 1, 2 (X1=D, X2=A, X3=D, X4=S) and 3 (X1=E, X2=G), respectively; (8) SEQ ID NOs: 1, 2 (X1=D, X2=A, X3=D, X4=S) and 3 (X1=D, X2=A), respectively; (9) SEQ ID NOs: 1, 2 (X1=E, X2=G, X3=E, X4=S) and 3 (X1=E, X2=G), respectively; (10) SEQ ID NOs: 1, 2 (X1=D, X2=A, X3=E, X4=S) and 3 (X1=E, X2=G), respectively; (11) SEQ ID SEQ ID NOs: 1, 2 (X1=E, X2=G, X3=D, X4=T) and 3 (X1=D, X2=A), respectively; or (12) SEQ ID NOs: 1, 2 (X1=D, X2=A, X3=D, X4=T) and 3 (X1=D, X2=A), respectively.

[0013] The isolated monoclonal heavy chain-only antibodies, or antigen-binding portions thereof, of the present disclosure may be selected from the group consisting of SEQ ID NOs: 4 (X1=S, X2=Q, X3=D, X4=G, X5=P; X1=S, X2=Q, X3=E, X4=G, X5=P; X1=S, X2=Q, X3=D, X4=A, X5=P; X1=S, X2=Q, X3=I, X4=G, X5=P; or X1=T, X2=G, X3=D, X4=G, X5=L), 5 (X1=E, X2=G, X3=D; X1=D, X2=A, X3=D; X1=E, X2=G, X3=E; or X1=D, X2=A, X3=E); 6 (X1=E, X2=G,X3=S;X1=D,X2=A,X3=S;X1=E,X2=G,X3=T;or X1=D,X2=A,X3=T),7,8(X1=F,X2=Y,X3=K,X4=A; 1=L,X2=Y,X3=R,X4=A;X1=L,X2=Y,X3=K,X4=R;or ;X1=L,X2=Y,X3=R,X4=A;X1=L,X2=Y,X3=K,X4=R;or 4=A;X1=L,X2=Y,X3=R,X4=A;X1=L,X2=Y,X3=K,X4=R;or X1=V, X2=W; or X1=F, X2=G). The amino acid sequences of SEQ ID NOs: 4 (X1=S, X2=Q, X3=D, X4=G, X5=P), 6 (X1=D, X2=A, X3=T) and 9 (X1=L, X2=Y, X3=K, X4=A) may be encoded by the nucleotide sequences of SEQ ID NOs: 23, 24 and 25, respectively.

[0014] The isolated monoclonal heavy chain-only antibodies, or antigen-binding portions thereof, of the present disclosure can comprise a constant region or functional fragment thereof linked to a variable region, wherein the C-terminus of the variable region is linked to the N-terminus of the constant region. The constant region may be a heavy chain constant region with enhanced FcR binding ability, such as a human IgG1 heavy chain constant region having the amino acid sequence set forth in SEQ ID NO:14, or a functional fragment thereof. The heavy chain constant region may also be a human IgG2 or IgG4 constant region, or a functional fragment thereof engineered to have improved FcR binding affinity. The amino acid sequence of SEQ ID NO:14 may be encoded by the nucleotide sequence of SEQ ID NO:26.

[0015] The present disclosure also provides bispecific molecules that can include a heavy chain-only antibody of the present disclosure, or an antigen-binding portion thereof, linked to a second functional moiety (e.g., a second antibody) having a binding specificity different from that of the antibody or antigen-binding portion thereof. The present disclosure also provides immunoconjugates that can include a heavy chain-only antibody of the present disclosure, or an antigen-binding portion thereof, linked to a therapeutic agent such as a cytotoxin, e.g., SN-38, or a radiolabel. The heavy chain-only antibody of the present disclosure, or an antigen-binding portion thereof, can be part of a chimeric antigen receptor (CAR). Immune cells, such as T cells and NK cells, that can construct chimeric antigen receptors are also provided. The heavy chain-only antibody of the present disclosure, or an antigen-binding portion thereof, can be encoded by or used in combination with an oncolytic virus.

[0016] The heavy chain-only antibody or antigen-binding portion thereof, immunoconjugate, or bispecific molecule can be radiolabeled and used in clinical imaging, for example, to track / detect tumor / cancer distribution, including metastatic tumor / cancer distribution. 3 These include, but are not limited to, H.

[0017] Nucleic acid molecules encoding the heavy chain-only antibodies, or antigen-binding portions thereof, bispecific molecules, immunoconjugates, or CARs of the present disclosure are also encompassed by the present disclosure, as are expression vectors that may contain such nucleic acids and host cells that may contain such expression vectors. Also provided are methods for preparing the anti-TROP2 heavy chain-only antibodies, or antigen-binding portions thereof, of the present disclosure using host cells, which may include the steps of (i) expressing the antibody or antigen-binding portion thereof in the host cell, and (ii) isolating the antibody or antigen-binding portion thereof from the host cell or a cell culture thereof.

[0018] Pharmaceutical compositions are also provided, which may comprise a heavy chain-only antibody, or antigen-binding portion thereof, immunoconjugate, bispecific molecule, oncolytic virus, CAR or CAR-T cell, nucleic acid molecule, expression vector, or host cell of the present disclosure, and a pharmaceutically acceptable carrier. In certain embodiments, the pharmaceutical composition may further comprise a therapeutic agent for treating a particular disease, such as an anti-cancer agent.

[0019] In yet another aspect, the present disclosure provides a method for treating a TROP2-related disease (e.g., excessive TROP2 expression) in a subject in need thereof, which may comprise administering a therapeutically effective amount of a pharmaceutical composition of the present disclosure to the subject. The disease may be a tumor or cancer. The tumor may be a solid tumor or a non-solid tumor, including, but not limited to, breast cancer, colorectal cancer, gastric adenocarcinoma, esophageal cancer, hepatocellular carcinoma, non-small cell lung cancer, small cell lung cancer, ovarian epithelial cancer, prostate cancer, pancreatic ductal adenocarcinoma, head and neck cancer, squamous cell carcinoma, renal cell carcinoma, bladder tumor, cervical cancer, endometrial cancer, follicular thyroid cancer, and glioblastoma multiforme. In certain embodiments, at least one additional anti-cancer antibody, such as an anti-VISTA antibody, anti-PD-1 antibody, anti-PD-L1 antibody, anti-LAG-3 antibody, anti-CTLA-4 antibody, anti-TIM 3 antibody, anti-STAT3 antibody, and / or anti-ROR1 antibody, may be further administered. In certain embodiments, the subject is human.

[0020] In another aspect, the present disclosure provides a method for cancer imaging in a subject in need thereof, comprising administering to the subject a radiolabeled anti-TROP2 heavy chain-only antibody or antigen-binding portion thereof, immunoconjugate, or bispecific molecule of the present disclosure. The method is used to track / detect the distribution of tumors or cancers with high TROP2 expression, including, but not limited to, esophageal squamous cell carcinoma, colorectal cancer, pancreatic cancer, colon cancer, papillary thyroid cancer, breast cancer, bladder cancer, etc. In certain embodiments, the subject is a human.

[0021] Other features and advantages of the instant disclosure will become apparent from the following detailed description and examples, which should not be construed as limiting. The contents of all references, Genbank entries, patents and published patent applications cited throughout this application are expressly incorporated herein by reference.

[0022] Accordingly, it is the object of the present invention not to encompass within its scope any previously known products, methods of making products, or methods of using products, and the Applicant hereby reserves the right to disclaim any previously known products, processes, or methods disclosed herein. Furthermore, it is noted that the present invention does not intend to encompass within its scope any products, processes, or methods of making products or methods of using products that do not meet the written description and enablement requirements of the USPTO (35 U.S.C. § 112, first paragraph) or the EPO (Article 83 EPC). The Applicant hereby discloses and reserves the right to disclaim any previously described products, methods of making products, or methods of using products. Compliance with Article 53(c) of the EPC and Rules 28(b) and (c) of the EPC is advantageous in the practice of the invention. All rights expressly reserved to explicitly disclaim any embodiments that are the subject of the Applicant's granted patents in this or any other lineage, or in any prior third-party applications. Nothing herein should be construed as a commitment.

[0023] It should be noted that in this disclosure, particularly in the claims and / or paragraphs, terms such as "comprises," "composed," "comprising," and the like can have the meaning ascribed to them in U.S. patent law, e.g., they mean "includes," "included," "including," and the like, and terms such as "consisting essentially of," "consists essentially of," and the like have the meaning ascribed to them in U.S. patent law, e.g., they allow for elements not expressly recited, but exclude elements found in the prior art or that affect the basic or novel characteristics of the invention. [Brief explanation of the drawings]

[0024] The following detailed description is given by way of example, and is not intended to limit the invention to only the particular embodiments described, and can be best understood in conjunction with the accompanying drawings, in which:

[0025] [Figure 1] 1 shows the binding ability of single domain antibodies 01-9F and 01-5A to human TROP2 in an indirect ELISA. [Figure 2] Figure 1 shows the ability of single domain antibodies 01-9F and 01-5A to inhibit binding to benchmark human TROP2 in a competitive ELISA. [Figure 3] Figure 1 shows the internalization-mediated cytotoxicity of DT3C conjugates of single domain antibodies 01-9F and 01-5A in 293F-TROP2 cells. [Figure 4] Figure 1 shows the internalization-mediated cytotoxicity of DT3C conjugates of heavy chain-only antibodies 01-9F-CDR-V5-Fc, 01-9F-CDR-V6-Fc, 01-9F-CDR-V9-Fc, and 01-9F-CDR-V11-Fc in 293F-TROP2 cells. [Figure 5]Figure 1 shows the internalization-mediated cytotoxicity of DT3C conjugates of humanized antibodies 01-9F-CDR-V11-V1-Fc, 01-9F-CDR-V11-V9-Fc, and 01-9F-CDR-V11-Fc on 293F-TROP2 cells. [Figure 6] FIG. 1 shows the binding ability of humanized antibody 01-9F-CDR-V11-V11-Fc to human TROP2 in capture ELISA. [Figure 7] 1 shows the binding ability of humanized antibody 01-9F-CDR-V11-V11-Fc to human TROP2 in indirect ELISA. [Figure 8] 1 shows the binding ability of humanized antibody 01-9F-CDR-V11-V11-Fc to cynomolgus monkey TROP2 in indirect ELISA. [Figure 9] 1 shows the binding ability of humanized antibody 01-9F-CDR-V11-V11-Fc to 293F-TROP2 cells expressing human TROP2 in a cell-based binding FACS assay. [Figure 10] 1 shows the ability of humanized antibody 01-9F-CDR-V11-V11-Fc to inhibit binding to benchmark human TROP2 in a competitive ELISA. [Figure 11A] 1 shows the results of a protein thermal shift assay for antibody 01-9F. [Figure 11B] 1 shows the results of a protein thermal shift assay of antibody 01-9F-CDR-V11-Fc. [Figure 11C] 1 shows the results of a protein thermal shift assay of antibody 01-9F-CDR-V11-Fc.

[0026] Detailed Description of the Invention In order that this disclosure may be more readily understood, certain terms are first defined. Additional definitions are set forth throughout the detailed description.

[0027] The term "TROP2" refers to tumor-associated calcium signaling protein 2, also known as epithelial glycoprotein-1, gastrointestinal antigen 733-1, and membrane component surface marker 1. The term "TROP2" may encompass variants, isoforms, homologs, orthologs, and paralogs. For example, an antibody specific for human TROP2 protein may, in certain cases, cross-react with TROP2 protein from species other than humans, such as monkeys. In other embodiments, an antibody specific for human TROP2 protein may be completely specific for human TROP2 protein and not cross-react with other species or types, or it may cross-react with TROP2 from certain other species but not all other species.

[0028] The term "human TROP2" refers to a TROP2 protein having an amino acid sequence of human origin, such as the amino acid sequence of human TROP2 set forth in SEQ ID NO: 20. The term "monkey TROP2" or "cynomolgus monkey TROP2" refers to a TROP2 protein having an amino acid sequence from Macaca nemestrina or macaca mulatta, such as the amino acid sequence having NCBI Accession No. XP_001114599.1 or XP_011762693.1.

[0029] In some cases, the term "antibody" may also refer specifically to the disclosed heavy chain-only antibodies or antigen-binding portions thereof. The term "heavy chain-only antibody" or "HCAb" refers to a functional antibody that contains only heavy chains but lacks the light chains typically found in four-chain immunoglobulins. Naturally occurring heavy chain-only antibodies are found, for example, in camelids (camels, llamas, alpacas, etc.). Each camelid heavy chain-only antibody contains a V H H domain, V H It contains a heavy chain variable region / domain, called an H fragment or single chain antibody (sdAb), and a heavy chain constant region. H H has the function of interacting with antigens. HThe heavy chain constant region contains three complementarity-determining regions (CDRs) and four framework regions (FRs), arranged in the following order from the amino terminus to the carboxy terminus: FR1, CDR1, FR2, CDR2, FR3, CDR3, FR4. The heavy chain constant region contains a hinge region, a CH2 domain, and a CH3 domain. H In chimeric or humanized heavy chain-only antibodies, the heavy chain constant region can comprise a typical IgG constant region, such as IgG1, IgG2, or IgG4. The constant region can mediate binding of the heavy chain-only antibody to host tissues or factors, including various cells of the immune system (e.g., effector cells) and the first component (C1q) of the classical complement system.

[0030] An "antigen-binding portion" when used in reference to a heavy chain-only antibody refers to one or more fragments of a heavy chain-only antibody that retain the ability to specifically bind to an antigen (e.g., TROP2). It has been shown that the antigen-binding function of a heavy chain antibody can be performed by fragments of a full-length heavy chain-only antibody. Examples of "antigen-binding portions of heavy chain-only antibodies" include (i) isolated complementarity-determining regions (CDRs); (ii) monovalent V H H fragment; (iii) two monovalent V H (iv) a bivalent fragment consisting of an H fragment; (iv) a V fragment linked to the CH2 domain or the CH2 and CH3 domains of the heavy chain constant region; H V linked to a partial heavy chain constant region, such as an H domain H (v) a monovalent fragment containing an H fragment; (v) two V fragments, each linked to a partial heavy chain constant region; H (vi) a bivalent fragment containing an H fragment; and (vi) a plurality of monovalent V fragments linked with or without a linker. HExamples include, but are not limited to, H domains. The term "single domain antibody," "sdAb," or "nanobody" refers to a single antigen-binding polypeptide comprising a single monomeric variable antibody domain with three complementarity-determining regions (CDRs), which can bind to an antigen without pairing with a corresponding CDR-containing polypeptide. In some cases, single domain antibodies are engineered from HCAbs of camelids and are also called VHH domains or fragments of HCAbs. Single domain antibodies are a type of antigen-binding portion of a heavy chain-only antibody. Also, V H H are sometimes called nanobodies. Camelid sdAbs are among the smallest known antigen-binding antibody fragments (see, e.g., Hamers-Casterman et al., Nature 363:446-8 (1993); Greenberg et al., Nature 374:168-73 (1995); Hassanzadeh-Ghassabeh et al., Nanomedicine (Lond), 8:1013-26 (2013)).

[0031] As used herein, an "isolated antibody" is intended to refer to an antibody that is substantially free of other antibodies with different antigenic specificities (e.g., an isolated antibody that specifically binds to TROP2 protein is substantially free of antibodies that specifically bind to antigens other than TROP2 protein). However, an isolated antibody that specifically binds to human TROP2 protein may have cross-reactivity to other antigens, e.g., TROP2 proteins from other species. Furthermore, an isolated antibody may be substantially free of other cellular material and / or chemicals.

[0032] As used herein, the term "camelid antibody" is intended to include antibodies having variable regions in which both the framework and CDR regions are derived from camelid germline immunoglobulin sequences. Furthermore, if the antibody contains a constant region, the constant region also is derived from camelid germline immunoglobulin sequences. Camelid antibodies of the present disclosure may include amino acid residues not encoded by camelid germline immunoglobulin sequences (e.g., mutations introduced by random or site-specific mutagenesis in vitro or by somatic mutation in vivo). However, as used herein, the term "camelid antibody" is not intended to include antibodies in which CDR sequences derived from the germline of other mammalian species have been grafted onto camelid framework sequences.

[0033] The term "chimeric antibody" refers to an antibody made by combining genetic material of non-human origin with genetic material of human origin, or, more generally, a chimeric antibody is an antibody that has genetic material from one species with genetic material from another species.

[0034] As used herein, the term "humanized antibody" refers to antibodies from non-human species in which the protein sequence has been altered to increase similarity to antibody variants naturally produced in humans.

[0035] As used herein, the term "monoclonal antibody" refers to an antibody obtained from a substantially homogeneous antibody population; i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations and / or minor post-translational modifications (e.g., isomerization, amidation). Monoclonal antibodies are highly specific, directed against a single antigenic site. Whereas polyclonal antibody preparations typically contain different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. In addition to their specificity, monoclonal antibodies are advantageous in that they are synthesized by a hybridoma culture, uncontaminated by other immunoglobulins. The modifier "monoclonal" indicates the character of the antibody as being obtained from a substantially homogeneous antibody population and is not to be construed as requiring production of the antibody by any particular method. For example, monoclonal antibodies to be used in accordance with the present invention can be produced by a variety of techniques, including, for example, hybridoma technology.

[0036] The term "isotype" refers to the antibody class (e.g., IgM or IgG1) that is encoded by heavy chain constant region genes.

[0037] As used herein, the terms "antibody that recognizes an antigen" and "antibody specific for an antigen" are used interchangeably with the term "antibody that specifically binds to an antigen."

[0038] As used herein, an antibody that "specifically binds to human TROP2" is intended to refer to an antibody that binds to human TROP2 protein (and optionally TROP2 proteins from one or more non-human species), but does not substantially bind to non-TROP2 proteins. Preferably, the antibody has a "high affinity," i.e., a binding affinity of 5.0x10 -8 M or less, preferably 1.0x10 -8 M or less, more preferably 2.0x10 -9 K below M D It binds to the human TROP2 protein.

[0039] As used herein, the term "does not substantially bind" to proteins or cells means that the protein or cell does not bind or does not bind with high affinity, i.e., less than 1.0 x 10 -6 M or more, preferably 1.0 × 10 -5 M or more, more preferably 1.0 × 10 -4 M or more, more preferably 1.0 × 10 -3 M or more, and even more preferably 1.0 × 10 -2 K over M D This means that there is binding to proteins or cells.

[0040] The term "high affinity" for an IgG antibody refers to an affinity of 1.0 × 10 for the target antigen. -6 M or less, preferably 5.0 × 10 -8 M or less, and even more preferably 1.0 × 10 -8 M or less, and even more preferably 1.0 × 10 -9 M or less, and even more preferably 1.0 × 10 -10 It refers to an antibody with a KD of 10 M or less. However, "high affinity" binding may vary for other antibody isotypes. For example, "high affinity" binding for an IgM isotype is considered to be 10 -6 M or less, preferably 10 -7 M or less, and even more preferably 10 -8 This refers to an antibody with a KD of M or less.

[0041] As used herein, the term "K assoc " or "K a " is intended to refer to the association rate of a particular antibody-antigen interaction, while the term "K" as used herein dis " or "K d " is intended to refer to the off-rate of a particular antibody-antigen interaction. D The term "K d and K. a The ratio of (i.e., K d / K a ) and is intended to be expressed as a molar concentration (M). DThe K value can be determined using methods well established in the art. D A preferred method for determining is by using surface plasmon resonance, preferably using a biosensor system such as a Biacore® system.

[0042] "EC 50 The term "half maximal effective concentration," also known as the half maximal effective concentration, refers to the concentration of antibody that induces a response halfway between the baseline and maximum after a specific exposure time.

[0043] "I C 50 The term "half maximal inhibitory concentration," also referred to as the concentration of an antibody that inhibits a specific biological or biochemical function by 50% compared to the absence of the antibody.

[0044] The term "subject" includes human or non-human animals. The term "non-human animal" includes all vertebrates, e.g., mammals and non-mammals, such as non-human primates, sheep, dogs, cats, cows, horses, chickens, amphibians, and reptiles, although mammals such as non-human primates, sheep, dogs, cats, cows, and horses are preferred.

[0045] The term "therapeutically effective amount" refers to an amount of an antibody or antigen-binding portion of the disclosure sufficient to prevent or ameliorate symptoms associated with a disease or condition (such as chronic inflammation) and / or reduce the severity of the disease or condition. A therapeutically effective amount is understood in the context of the condition being treated, where the actual effective amount is readily discernible by one of ordinary skill in the art.

[0046] Various aspects of the disclosure are described in further detail in the following subsections.

[0047] The heavy chain-only antibodies or antigen-binding portions thereof of the present disclosure specifically bind to human TROP2 with similar, if not higher, affinity / binding capacity for human and / or monkey TROP2 and have similar, if not higher, internalization activity compared to prior art anti-TROP2 antibodies such as sacituzumab (the antibody portion of IMMU-132).

[0048] The antibodies, or antigen-binding portions thereof, of the present disclosure are camelid, chimeric, and humanized. The antibodies of the present disclosure are heavy chain-only antibodies.

[0049] [Table 1] [Table 2]

[0050] The antibodies, or antigen-binding portions thereof, of the present disclosure are monoclonal antibodies that have been structurally and chemically characterized as described below and in the Examples below. The amino acid sequence ID numbers of the variable regions and CDRs of the present disclosure are summarized in Table 1, and some antibodies have the same V H The constant region of the antibody may be, for example, a heavy chain constant region consisting of the amino acid sequence of SEQ ID NO: 14 or a functional fragment thereof. The antibody of the present disclosure may also comprise a human IgG1, IgG2, or IgG4 heavy chain constant region.

[0051] The variable region CDRs in Table 1 are defined by the Kabat numbering system, however, as is well known in the art, CDR regions can also be determined based on the variable region sequence by other systems, such as Chothia, and the IMGT, AbM, or Contact numbering systems / methods.

[0052] V of other anti-TROP2 antibodies that bind to human TROP2 H The H sequence (or CDR sequence) of the anti-TROP2 antibody of the present disclosure H One can "mix and match" with H sequences (or CDR sequences).

[0053] Thus, in one embodiment, an antibody of the present disclosure, or an antigen-binding portion thereof, may comprise a variable region that may comprise an amino acid sequence set forth above in Table 1, wherein the antibody specifically binds human TROP2.

[0054] In another embodiment, an antibody of the present disclosure, or an antigen-binding portion thereof, may comprise the CDR1, CDR2, and CDR3 regions of the heavy chain variable region set forth above in Table 1, wherein the antibody specifically binds to human TROP2.

[0055] In yet another embodiment, the antibody, or antigen-binding portion thereof, comprises the CDR2 region of an anti-TROP2 antibody combined with the CDRs of another antibody that binds to human TROP2, e.g., CDR1 and / or CDR3 from the variable region of a different anti-TROP2 antibody.

[0056] Furthermore, it is well known in the art that the CDR3 domain can solely determine the binding specificity of an antibody to its cognate antigen, independently of the CDR1 and / or CDR2 domains, and that multiple antibodies with identical binding specificity can be predictably generated based on a common CDR3 sequence. For example, Klimka et al.,British J.of Cancer 83(2):252-260(2000);Beiboer et al.,J.Mol.Biol.296:833-849(2000);Rader et al.,Proc.Natl.Acad.Sci.USA95:8910-8915(1998);Barbas et al. al.,J. Am.Chem.Soc.116:2161-2162(1994);Barbas et al.,Proc.Natl.Acad.Sci.USA92:2529-2533(1995);Ditzel et al.,J.Immunol.157:739-749(1996);Berezov et al.,BIAjournal 8:Scientific Review 8(2001);Igarashi et al. See, e.g., Bourgeois et al., J. Virol 72:807-10 (1998); Levi et al., Proc. Natl. Acad. Sci. USA 90:4374-8 (1993); Polymenis and Stoller, J. Immunol. 152:5218-5329 (1994), and Xu and Davis, Immunity 13:37-45 (2000). See also U.S. Patent Nos. 6,951,646; 6,914,128; 6,090,382; 6,818,216; 6,156,313; 6,827,925; 5,833,943; 5,762,905; and 5,760,185, each of which is incorporated herein by reference in its entirety.

[0057] The antibodies of the present disclosure have one or more of the following functional properties described above, such as high affinity binding to human TROP2.

[0058] In various embodiments, the antibody can be, for example, a camelid, chimeric, or humanized antibody.

[0059] As used herein, the term "conservative sequence modifications" is intended to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative modifications include amino acid substitutions, additions, and deletions. Modifications can be introduced into the antibodies of the present disclosure by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions are those that replace an amino acid residue with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan), nonpolar side chains (e.g., alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine), beta-branched side chains (e.g., threonine, valine, isoleucine), and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus, one or more amino acid residues in the CDR regions of an antibody of the disclosure can be substituted with another amino acid residue from the same side chain family, and the altered antibodies can be tested for retained function (i.e., as defined above) using the functional assays described herein.

[0060] The antibodies of the present disclosure may be any of the V and VIII anti-TROP2 antibodies of the present disclosure. H Modified antibodies can be engineered using an antibody having a variable region (i.e., V HH), for example, by modifying one or more residues in one or more CDR regions and / or one or more framework regions. Additionally, or alternatively, antibodies can be engineered by modifying residues in the constant region(s), for example, to alter the effector function(s) of the antibody.

[0061] In certain embodiments, CDR grafting can be used to engineer the variable region of an antibody. Antibodies interact with target antigens primarily through amino acid residues located in their three complementarity-determining regions (CDRs). Therefore, the amino acid sequences within the CDRs are more diverse among individual antibodies than the sequences outside the CDRs. Because the CDR sequences are responsible for most antibody-antigen interactions, it is possible to express recombinant antibodies that mimic the properties of a particular naturally occurring antibody by constructing expression vectors containing the CDR sequences from that particular naturally occurring antibody grafted onto framework sequences from another antibody with different properties (e.g., Riechmann et al., (1998) Nature 332:323-327; Jones et al., (1986) Nature 321:522-525; Queen et al., (1989) Proc. Natl. Acad. USA 86:10029-10033; U.S. Patent Nos. 5,225,539; 5,530,101; 5,585,089; 5,693,762 and 6,180,370).

[0062] Accordingly, another embodiment of the present disclosure relates to isolated monoclonal antibodies, or antigen-binding portions thereof, which can comprise variable regions that can include CDR1, CDR2, and CDR3 sequences that can comprise sequences of the present disclosure, as described above. These antibodies contain the CDR sequences of the monoclonal antibodies of the present disclosure, but they can comprise different framework sequences.

[0063] Such framework sequences can be obtained from public DNA databases or published literature containing germline antibody gene sequences. For example, germline DNA sequences of human heavy and light chain variable region genes can be obtained from the "VBase" human germline sequence database (available on the Internet at www.mrc-cpe.cam.ac.uk / vbase), as well as Kabat et al., (1991), cited supra; Tomlinson et al., (1992) J. Mol. Biol. 227:776-798; and Cox et al., (1994) Eur. J. Immunol. 24:827-836, supra, the contents of each of which are expressly incorporated herein by reference. As another example, germline DNA sequences of human heavy and light chain variable region genes can be found in the Genbank database. For example, the following heavy chain germline sequences found in the HCo7 HuMAb mouse are available at the attached Genbank Accession Nos.: 1-69 (NG--0010109, NT--024637 & BC070333), 3-33 (NG--0010109 & NT--024637) and 3-7 (NG--0010109 & NT--024637). As another example, the following heavy chain germline sequences found in the HCo12 HuMAb mouse are available under the attached Genbank Accession Nos: 1-69 (NG--0010109, NT--024637 & BC070333), 5-51 (NG--0010109 & NT--024637), 4-34 (NG--0010109 & NT--024637), 3-30.3 (CAJ556644) & 3-23 (AJ406678).

[0064] The antibody protein sequence is compared against compiled protein sequence databases using one of the sequence similarity search methods known to those skilled in the art as Gapped BLAST (Altschul et al., (1997), supra).

[0065] Preferred framework sequences for use in the antibodies of this disclosure are those that are structurally similar to the framework sequences used by the antibodies of this disclosure. H The H CDR1, CDR2, and CDR3 sequences can be grafted into framework regions having the same sequence as found in the germline immunoglobulin gene from which the framework sequences are derived, or the CDR sequences can be grafted into framework regions that contain one or more mutations compared to the germline sequence. For example, in some cases, it has been found to be beneficial to mutate residues within the framework regions to maintain or enhance the antigen-binding ability of the antibody (see, e.g., U.S. Patent Nos. 5,530,101; 5,585,089; 5,693,762; and 6,180,370).

[0066] Another type of variable region modification is the V H H. Mutation of amino acid residues within the CDR1, CDR2, and / or CDR3 regions to thereby improve one or more binding characteristics (e.g., affinity) of the antibody of interest. Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce the mutations, and the effect on antibody binding or other functional properties of interest can be assessed by in vitro or in vivo assays, as known in the art. Preferably, conservative modifications (as known in the art) are introduced. Mutations can be amino acid substitutions, additions, or deletions, but are preferably substitutions. Furthermore, typically, no more than one, two, three, four, or five residues within the CDR regions are altered.

[0067] Thus, in another embodiment, the present disclosure provides an isolated anti-TROP2 monoclonal antibody, or antigen-binding portion thereof, which may comprise a variable region comprising: (a) a CDR1 region that may comprise a sequence of the present disclosure, or an amino acid sequence having one, two, three, four, or five amino acid substitutions, deletions, or additions; (b) a CDR2 region that may comprise a sequence of the present disclosure, or an amino acid sequence having one, two, three, four, or five amino acid substitutions, deletions, or additions; and (c) a CDR3 region that may comprise a sequence of the present disclosure, or an amino acid sequence having one, two, three, four, or five amino acid substitutions, deletions, or additions.

[0068] The engineered antibodies of the present disclosure may be modified, for example, by modifying the V H This includes modifications made to framework residues within H. Typically, such framework modifications are made to reduce the immunogenicity of the antibody. For example, one approach is to "backmutate" one or more framework residues to their corresponding germline residues. More specifically, antibodies that have undergone somatic mutation may contain framework residues that differ from the germline sequence from which the antibody is derived. Such residues can be identified by comparing the antibody framework sequence to the germline sequence from which the antibody is derived.

[0069] Another type of framework modification involves mutating one or more residues within the framework regions, or within one or more CDR regions, to remove T-cell epitopes, thereby reducing the potential immunogenicity of the antibody. This approach, also known as "deimmunization," is described in further detail in U.S. Patent Publication No. 20030153043.

[0070] Additionally, or as an alternative to modifications made within the framework or CDR regions, antibodies of the disclosure can be engineered to contain modifications within the Fc region, typically to alter one or more functional properties of the antibody, such as serum half-life, complement fixation, Fc receptor binding, and / or antigen-dependent cellular cytotoxicity. Further, antibodies of the disclosure can be chemically modified (e.g., one or more chemical moieties can be attached to the antibody) or modified to alter its glycosylation, again to alter one or more functional properties of the antibody.

[0071] In one embodiment, C H1 The hinge region of C is modified such that the number of cysteine ​​residues in the hinge region is altered, e.g., increased or decreased. This approach is further described in U.S. Pat. No. 5,677,425. H1 The number of cysteine ​​residues in the hinge region of the antibody is altered to, for example, facilitate assembly of the light and heavy chains or to increase or decrease the stability of the antibody.

[0072] In another embodiment, the Fc hinge region of the antibody is mutated to decrease the biological half-life of the antibody. More specifically, one or more amino acid mutations are made to the C of the Fc hinge fragment such that the antibody has impaired staphylococcal protein A (SpA) binding compared to native Fc hinge domain SpA binding. H2 -C H3 The method is described in more detail in U.S. Patent No. 6,165,745.

[0073] In yet another embodiment, the glycosylation of an antibody is modified. For example, a glycosylated antibody can be generated (i.e., the antibody lacks glycosylation). Glycosylation can be altered, for example, to increase the affinity of the antibody for an antigen. Such carbohydrate modifications can be achieved, for example, by altering one or more glycosylation sites within the antibody sequence. For example, one or more amino acid substitutions can be made to remove one or more variable region framework glycosylation sites, thereby eliminating glycosylation at that site. Such aglycosylation can increase the affinity of the antibody for an antigen. See, e.g., U.S. Patent Nos. 5,714,350 and 6,350,861.

[0074] Additionally, antibodies can be produced with altered glycosylation types, such as hypofucosylated antibodies with reduced amounts of fucosyl residues or antibodies with increased double-chain GlcNac structures. Such glycosylation modifications have been shown to increase or decrease the ADCC activity of antibodies. Such glycosylation modifications can be achieved, for example, by expressing the antibody in a host cell with an altered glycosylation machinery. Cells with altered glycosylation machinery have been described in the art and can be used as host cells to express the recombinant antibodies of the present disclosure, thereby producing antibodies with altered glycosylation. For example, the cell lines Ms704, Ms705, and Ms709 lack the fucosyltransferase gene FUT8 (α(1,6)-fucosyltransferase), and antibodies expressed in the Ms704, Ms705, and Ms709 cell lines will lack fucose on their carbohydrates. The Ms704, Ms705, and Ms709 FUT8- / - cell lines were generated by targeted disruption of the FUT8 gene in CHO / DG44 cells using two replacement vectors (see U.S. Patent Publication No. 20040110704 and Yamane-Onuki et al. (2004) Biotechnol Bioeng 87:614-22). As another example, EP 1,176,195 describes cell lines in which the FUT8 gene, encoding fucosyltransferase, is functionally disrupted, resulting in antibodies expressed in such cell lines exhibiting hypofucosylation by reducing or eliminating α-1,6-linkage-related enzymes. EP 1,176,195 also describes cell lines with reduced or no enzymatic activity for adding fucose to N-acetylglucosamine attached to the Fc region of antibodies, such as the rat myeloma cell line YB2 / 0 (ATCC CRL 1662). PCT Publication WO 03 / 035835 describes a mutant CHO cell line, Lec13 cells, that has a reduced ability to attach fucose to Asn(297)-linked carbohydrates, which also results in hypofucosylation of antibodies expressed in the host cells (see also Shields et al., (2002) J. Biol. Chem. 277:26733-26740).Antibodies with modified glycosylation profiles can also be produced in chicken eggs, as described in PCT Publication WO 06 / 089231. Alternatively, antibodies with modified glycosylation profiles can be produced in plant cells, such as Lemna. Methods for producing antibodies in plant systems are disclosed in U.S. patent application Ser. No. 040989 / 314911, filed August 11, 2006, to Alston & Bird LLP. Fucose residues on antibodies can be cleaved using a fucosidase enzyme; for example, the fucosidase α-L-fucosidase removes fucosyl residues from antibodies (see also Tarentino et al. (1975) Biochem. 14:5516-23).

[0075] Another modification of the antibodies herein contemplated by the present disclosure is pegylation. Antibodies can be pegylated, for example, to increase the biological (e.g., serum) half-life of the antibody. To pegylate an antibody, the antibody or fragment thereof is typically reacted with polyethylene glycol (PEG), such as a reactive ester or aldehyde derivative of PEG, under conditions that result in one or more PEG groups being attached to the antibody or antibody fragment. Preferably, pegylation is carried out via an acylation reaction or an alkylation reaction with a reactive PEG molecule (or an analogous reactive water-soluble polymer). As used herein, the term "polyethylene glycol" is intended to include any of the forms of PEG that have been used to derivatize other proteins, such as mono(C1-C10)alkoxy- or aryloxy-polyethylene glycol or polyethylene glycol-maleimide. In certain embodiments, the antibody to be pegylated is an aglycosylated antibody. Methods for pegylating proteins are known in the art and can be applied to the antibodies of the present disclosure. See, for example, EP 0 154 316 and EP 0 401 384.

[0076] The antibodies of the present disclosure can be characterized by their various physical properties in order to detect and / or distinguish between different classes thereof.

[0077] For example, an antibody may contain one or more glycosylation sites in the variable region. Such glycosylation sites may increase the immunogenicity of the antibody or alter the antibody's pK, resulting in altered antigen binding (Marshall et al., (1972) Annu Rev Biochem 41:673-702; Gala and Morrison (2004) J Immunol 172:5489-94; Wallick et al., (1988) J Exp Med 168:1099-109; Spiro (2002) Glycobiology 12:43R-56R; Parekh et al., (1985) Nature 316:452-7; Mimura et al., (2000) Mol Immunol 37:697-706). Glycosylation is known to occur at motifs containing the NXS / T sequence. In some instances, it may be preferable to have an anti-TROP2 antibody that does not contain variable region glycosylation, which can be achieved by selecting an antibody that does not contain glycosylation motifs in the variable region or by mutating residues in the glycosylated region.

[0078] In a preferred embodiment, the antibody does not contain an asparagine isomerization site: deamidation of asparagine occurs at NG or DG sequences, leading to the generation of isoaspartic acid residues that introduce linkages into the polypeptide chain and reduce its stability (isoaspartic acid effect).

[0079] Each antibody has a unique isoelectric point (pI), generally ranging from pH 6 to 9.5. The pI of IgG1 antibodies generally falls within the pH range of 7-9.5, while the pI of IgG4 antibodies generally falls within the pH range of 6-8. Antibodies with pIs outside the normal range are suspected to be susceptible to unfolding or instability under in vivo conditions. Therefore, anti-TROP2 antibodies with pIs within the normal range are preferred. This can be achieved by selecting antibodies with pIs within the normal range or by mutating charged surface residues.

[0080] In another aspect, the present disclosure provides nucleic acid molecules encoding the variable regions, i.e., CDRs, of the antibodies of the present disclosure. The nucleic acids can be present in whole cells, in a cell lysate, or in a partially purified or substantially pure form. A nucleic acid is "isolated" or "substantially purified" if it has been purified away from other cellular components or other contaminants, such as other cellular nucleic acids or proteins, by standard techniques. The nucleic acids of the present disclosure can be, for example, DNA or RNA, and may or may not contain intron sequences. In a preferred embodiment, the nucleic acid is a cDNA molecule.

[0081] Nucleic acids of the present disclosure can be obtained using standard molecular biology techniques. For antibodies expressed by hybridomas (e.g., hybridomas prepared from transgenic mice carrying human immunoglobulin genes, as further described below), cDNA encoding the heavy chain of the antibody made by the hybridoma can be obtained by standard PCR amplification or cDNA cloning techniques. For antibodies obtained from an immunoglobulin gene library (e.g., using phage display deep Ls), nucleic acids encoding such antibodies can be recovered from the gene library.

[0082] Preferred nucleic acid molecules of the present disclosure include the V H These include those encoding H sequences or CDRs. H Once the DNA fragments encoding the H segment are obtained, these DNA fragments can be used to convert, for example, the variable region genes into full-length antibody chain genes or V H It can be further manipulated by standard recombinant DNA techniques to convert it into an H fragment gene.

[0083] V H The isolated DNA encoding the H region is H The DNA encoding the heavy chain constant region (C H1 , C H2 , C H3The heavy chain constant region can be converted into a full-length heavy chain gene by operatively linking it to another DNA molecule encoding the heavy chain constant region. The sequences of human heavy chain constant region genes are known in the art, and DNA fragments encompassing these regions can be obtained by standard PCR amplification. The heavy chain constant region can be an IgG1, IgG2, IgG3, IgG4, IgA, IgE, IgM, or IgD constant region, but is most preferably an IgG1 or IgG4 constant region.

[0084] The monoclonal antibodies (mAbs) of the present disclosure can be produced using the well-known somatic cell hybridization (hybridoma) technique of Kohler and Milstein (1975) Nature 256:495. Other embodiments for producing monoclonal antibodies include viral or oncogenic transformation of B lymphocytes and phage display. Chimeric or humanized antibodies are also well known in the art. See, e.g., U.S. Patent Nos. 4,816,567; 5,225,539; 5,530,101; 5,585,089; 5,693,762; and 6,180,370, the contents of which are expressly incorporated herein by reference in their entireties.

[0085] Antibodies of the disclosure can also be produced in host cell transfectants, for example, using a combination of recombinant DNA technology and gene transfection methods as are well known in the art (e.g., Morrison, S. (1985) Science 229:1202). In one embodiment, DNA encoding a partial- or full-length heavy chain, obtained by standard molecular biology techniques, is inserted into one or more expression vectors such that the gene is operably linked to transcriptional and translational control sequences. In this context, the term "operably linked" means that the antibody gene is ligated into a vector such that transcriptional and translational control sequences within the vector perform their intended function of regulating the transcription and translation of the antibody gene.

[0086] The term "regulatory sequence" is intended to include promoters, enhancers, and other expression control elements (e.g., polyadenylation signals) that control the transcription or translation of antibody genes. Such regulatory sequences are described, for example, in Goeddel (Gene Expression Technology. Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990)). Preferred regulatory sequences for mammalian host cell expression include promoters and / or enhancers derived from viral elements that direct high-level protein expression in mammalian cells, such as cytomegalovirus (CMV), simian virus 40 (SV40), and adenovirus, e.g., the adenovirus major late promoter (AdMLP) and poly(A) virus enhancer. Alternatively, non-viral regulatory sequences, such as the ubiquitin promoter or β-globin promoter, can be used. Further examples include regulatory elements composed of sequences from different sources, such as the SRα promoter system (Takebe et al., (1988) Mol. Cell. Biol. 8:466-472), which contains sequences from the SV40 early promoter and the long terminal repeat of human T-cell leukemia virus type 1. Expression vectors and expression control sequences are selected to be compatible with the expression host cell used.

[0087] In addition to the antibody chain genes and regulatory sequences, the recombinant expression vectors of the disclosure can carry additional sequences, such as sequences that regulate replication of the vector in host cells (e.g., origins of replication) and selectable marker genes. The selectable marker gene facilitates selection of host cells into which the vector has been introduced (see, e.g., U.S. Pat. Nos. 4,399,216; 4,634,665; and 5,179,017). For example, typically the selectable marker gene confers resistance to drugs, such as G418, hygromycin, or methotrexate, on the host cells into which the vector has been introduced. Preferred selectable marker genes include the dihydrofolate reductase (DHFR) gene (for use in dhfr-host cells with methotrexate selection / amplification) and the neo gene (for G418 selection).

[0088] For expression of the heavy chain, the expression vector(s) encoding the heavy chain are transfected into a host cell by standard techniques. The various forms of the term "transfection" are intended to encompass a wide variety of techniques commonly used for introducing exogenous DNA into prokaryotic or eukaryotic host cells, such as electroporation, calcium phosphate precipitation, DEAE-dextran transfection, etc. While it is theoretically possible to express the antibodies of the disclosure in either prokaryotic or eukaryotic host cells, expression of the antibodies in eukaryotic cells, particularly mammalian host cells, is most preferred, as such eukaryotic cells, particularly mammalian cells, are more likely than prokaryotic cells to assemble and secrete a properly folded and immunologically active antibody.

[0089] Preferred mammalian host cells for expressing the recombinant antibodies of the disclosure include Chinese hamster ovary (CHO) cells (including, for example, the dhfr-CHO cells described by Urlaub and Chasin (1980) Proc. Natl. Acad. Sci. USA 77:4216-4220, used with the DHFR selectable marker described by R. J. Kaufman and P. A. Sharp (1982) J. Mol. Biol. 159:601-621), NSO myeloma cells, COS cells, and SP2 cells. Another preferred expression system, particularly for use with NSO myeloma cells, is the GS gene expression system disclosed in WO 87 / 04462, WO 89 / 01036, and EP 338,841. When a recombinant expression vector encoding an antibody gene is introduced into a mammalian host cell, the antibody can be produced by culturing the host cell for a period of time sufficient to allow for expression of the antibody in the host cell, or more preferably, secretion of the antibody into the culture medium in which the host cell is grown. Antibodies can be recovered from the culture medium using standard protein purification methods.

[0090] In another aspect, the present disclosure features bispecific molecules that can include one or more antibodies of the present disclosure linked to at least one other functional molecule, e.g., another peptide or protein (e.g., another antibody or a ligand for a receptor), to generate a bispecific molecule that binds to at least two different binding sites or target molecules. Thus, as used herein, "bispecific molecule" includes molecules with three or more specificities.

[0091] In one embodiment, the bispecific molecule has a third specificity in addition to an Fc binding specificity and an anti-TROP2 binding specificity.

[0092] The third specificity may be for PD-1. In one embodiment, the antibodies or antigen-binding portions thereof of the invention are used to determine the presence and expression of TROP2 in tissue. In one embodiment, the diagnosis indicates a prognosis and / or indicates treatment and / or follow-up. For example, TROP2 signaling is targeted for tumor treatment. In one embodiment, the antibodies or antigen-binding portions of the invention are employed in diagnostic kits or methods for determining the prognosis and appropriate treatment and follow-up of a TROP2-associated tumor or cancer.

[0093] The antibodies of the present disclosure can be conjugated to a therapeutic agent, a cytotoxin, or a radiolabel to form an immunoconjugate. The cytotoxin can be, for example, a recombinant protein called DT3C, which has the amino acid sequence of SEQ ID NO: 22.

[0094] Oncolytic viruses preferentially infect and kill cancer cells. The antibodies of the present disclosure can be used in combination with oncolytic viruses. Alternatively, oncolytic viruses encoding the antibodies of the present disclosure can be introduced into the human body.

[0095] Also herein, anti-TROP2 V H A chimeric antigen receptor (CAR) containing the H fragment was provided, and an anti-TROP2 V H H can comprise the CDRs and heavy chain variable region described herein.

[0096] Anti-TROP2 CARs are (a) anti-TROP2 V H It may consist of (a) an extracellular antigen-binding domain, which may include H; (b) a transmembrane domain; and (c) an intracellular signaling domain.

[0097] CARs may contain a signal peptide at the N-terminus of the extracellular antigen-binding domain that guides the nascent receptor to the endoplasmic reticulum, and a hinge peptide at the N-terminus of the extracellular antigen-binding domain that enhances receptor binding. CARs preferably comprise a primary intracellular signaling domain and one or more costimulatory signaling domains in the intracellular signaling domain. The most commonly used and most effective intracellular primary signaling domain is the ITAM-containing CD3-zeta cytoplasmic domain, whose phosphorylation leads to T cell activation. The costimulatory signaling domain may be derived from costimulatory proteins such as CD28, CD137, and OX40.

[0098] CARs may further be supplemented with factors that enhance T cell expansion, persistence, and anti-tumor activity, such as cytokines and costimulatory ligands.

[0099] Also provided are engineered immune effector cells, which may comprise the CARs provided herein. In certain embodiments, the immune effector cells are T cells, NK cells, peripheral blood mononuclear cells (PBMCs), hematopoietic stem cells, pluripotent stem cells, or embryonic stem cells. In certain embodiments, the immune effector cells are T cells.

[0100] In another aspect, the present disclosure provides pharmaceutical compositions that may include one or more antibodies (or antigen-binding portions thereof, bispecifics, CAR-T cells, oncolytic viruses, immunoconjugates, or alternatively, nucleic acid molecules or expression vectors capable of expressing same) of the present disclosure formulated together with a pharmaceutically acceptable carrier. The antibodies (or antigen-binding portions thereof, bispecifics, CAR-T cells, oncolytic viruses, immunoconjugates, or alternatively, nucleic acid molecules or expression vectors of the present disclosure capable of expressing same) may be administered separately when the composition includes multiple antibodies (or antigen-binding portions thereof, bispecifics, CAR-T cells, oncolytic viruses, immunoconjugates, or alternatively, nucleic acid molecules or expression vectors of the present disclosure capable of expressing same). The compositions may optionally include one or more additional pharmaceutically active ingredients, such as another antibody or an agent, such as an anti-tumor drug.

[0101] Pharmaceutical compositions may be comprised of any number of excipients. Excipients that can be used include carriers, surfactants, thickeners or emulsifiers, solid binders, dispersing or suspending aids, solubilizers, colorants, flavoring agents, coatings, disintegrants, lubricants, sweeteners, preservatives, isotonicity agents, and combinations thereof. The selection and use of suitable excipients is taught in Gennaro, ed., Remington: The Science and Practice of Pharmacy, 20th Ed. (Lippincott Williams & Wilkins 2003), the disclosure of which is incorporated herein by reference.

[0102] Preferably, the pharmaceutical compositions are suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal, or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active ingredient may be coated with a material to protect it from the action of acids and other natural conditions that may inactivate it. As used herein, the term "parenteral administration" refers to modes of administration other than enteral and topical administration, usually by injection, and includes, but is not limited to, intravenous, intramuscular, intraarterial, intrathecal, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, intraarticular, putamen, subarachnoid, intraspinal, epidural, and intraperitoneal injection and infusion. Alternatively, antibodies of the present disclosure can be administered parenterally, e.g., by topical, epidermal, or mucosal administration routes, e.g., intranasal, oral, vaginal, rectal, sublingual, or topical.

[0103] The pharmaceutical compositions can be in the form of sterile aqueous solutions or dispersions or can be formulated in ordered structures suitable to high drug concentration, such as microemulsions or liposomes.

[0104] The amount of active ingredient which can be combined with a carrier material to produce a single dosage form will vary depending upon the subject being treated and the particular mode of administration, but will generally be that amount of the composition which produces a therapeutic effect. Generally, out of 100%, this amount will range from about 0.01% to about 99% of the active ingredient.

[0105] Dosage regimens are adjusted to provide the optimum desired response (e.g., therapeutic response). For example, a single bolus can be administered, several divided doses can be administered over time, or the dosage can be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. For ease of administration and uniformity of dosage, it is particularly advantageous to formulate parenteral compositions in dosage unit form. As used herein, dosage unit form refers to physically discrete units suitable as unitary dosages for the subject to be treated, each unit containing a predetermined amount of active ingredient calculated to produce the desired therapeutic effect in association with the necessary pharmaceutical carrier. Alternatively, antibodies can be administered as sustained-release formulations, in which case less frequent administration is required.

[0106] For administration of the composition, the dosage may range from about 0.0001 to 100 mg / kg. An exemplary treatment regime involves administration once a month.

[0107] A "therapeutically effective amount" of an anti-TROP2 antibody, or antigen-binding portion thereof, or a bispecific, CAR-T cell, oncolytic virus, or immunoconjugate of the present disclosure preferably results in a decrease in the severity of disease symptoms, an increase in the frequency or duration of disease-free periods, or prevention of disability or injury due to disease affliction. For example, for the treatment of a cancer-bearing subject, a "therapeutically effective amount" preferably eliminates inflammation by at least about 20%, more preferably at least about 40%, even more preferably at least about 60%, and even more preferably at least about 80% relative to an untreated subject.

[0108] The pharmaceutical composition can be a controlled release formulation, including implants, transdermal patches, and microencapsulated delivery systems.Biodegradable biocompatible polymers, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid, can be used.See, for example, "Sustained and Controlled Release Drug Delivery Systems," JR Robinson, ed., Marcel Dekker, Inc., New York, 1978.

[0109] Therapeutic compositions can be administered via medical devices such as: (1) needleless hypodermic syringes (e.g., U.S. Pat. Nos. 5,399,163; 5,383,851; 5,312,335; 5,064,413; 4,941,880; 4,790,824; 4,596,556); (2) microinfusion pumps (U.S. Pat. No. 4,487,603); (3) transdermal devices (U.S. Pat. Nos. 5,486,194; 4,486,194); (4) infusion devices (U.S. Pat. Nos. 4,447,233 and 4,447,224); and (5) osmotic devices (U.S. Pat. Nos. 4,439,196 and 4,475,196), the disclosures of which are incorporated herein by reference.

[0110] In certain embodiments, the monoclonal antibodies of the present disclosure can be formulated to ensure proper distribution in vivo. For example, to ensure that therapeutic antibodies of the present disclosure, or antigen-binding portions thereof, cross the blood-brain barrier, they can be formulated in liposomes, which may additionally contain a targeting moiety to enhance selective delivery to specific cells or organs. For example, U.S. Patent Nos. 4,522,811; 5,374,548; 5,416,016; and 5,399,331; al.,(1988)Biochem.Biophys.Res.Commun.153:1038;Bloeman et al.,(1995)FEBS Lett.357:140;M.Owais et al.,(1995)Antimicrob.Agents Chemother.39:180;Briscoe et al. al.,(1995)Am.J.Physiol.1233:134;Schreier et al.,(1994)J.Biol.Chem.269:9090;Keinanen and See Laukkanen (1994) FEBS Lett. 346:123; and Killion and Fidler (1994) Immunomethods 4:273.

[0111] Pharmaceutical compositions that may include antibodies or antigen-binding portions thereof, or bispecifics, CAR-T cells, oncolytic viruses, immunoconjugates, or alternatively the disclosed nucleic acid molecules or vectors capable of expressing the same of the present disclosure have numerous in vitro and in vivo utilities, including, for example, the treatment of tumors with excessive TROP2 signaling.

[0112] Given that TROP2 is associated with tumor cell proliferation, the present disclosure provides a method for treating a TROP2-associated tumor or cancer, which may include administering a pharmaceutical composition of the present disclosure to a subject. The tumor may be a solid tumor or a hematological tumor, including, but not limited to, breast cancer, colorectal cancer, gastric adenocarcinoma, esophageal cancer, hepatocellular carcinoma, non-small cell lung cancer, small cell lung cancer, ovarian epithelial cancer, prostate cancer, pancreatic ductal adenocarcinoma, head and neck cancer, squamous cell carcinoma, renal cell carcinoma, bladder tumor, cervical cancer, endometrial cancer, follicular thyroid cancer, and glioblastoma multiforme. In certain embodiments, at least one additional anti-cancer antibody, such as an anti-VISTA antibody, anti-PD-1 antibody, anti-PD-L1 antibody, anti-LAG-3 antibody, anti-CTLA-4 antibody, anti-TIM 3 antibody, anti-STAT3 antibody, and / or anti-ROR1 antibody, may be further administered. In certain embodiments, the subject is human.

[0113] In another aspect, the present disclosure provides a method of combination therapy in which a pharmaceutical composition of the present disclosure is co-administered with one or more additional antibodies effective in inhibiting tumor growth in a subject. In one embodiment, the present disclosure provides a method for inhibiting tumor growth in a subject, which may include administering to the subject a pharmaceutical composition of the present disclosure and one or more additional antibodies, such as an anti-OX40 antibody, an anti-TIM-3 antibody, an anti-CD137 antibody, an anti-GITR antibody, an anti-LAG-3 antibody, an anti-PD-L1 antibody, and an anti-PD-1 antibody. In certain embodiments, the subject is human. TROP2 pathway blockade may also be combined with standard cancer treatments. For example, TROP2 pathway blockade can be combined with LAG-3 and / or PD-1 blockade, as well as with a chemotherapy regimen. For example, a chemotherapeutic agent can be administered together with the anti-TROP2 antibody, and the chemotherapeutic agent may be a cytotoxic agent. For example, epirubicin, oxaliplatin, and 5-FU are administered to patients receiving anti-TROP2 therapy. Optionally, the combination of anti-TROP2 and one or more additional antibodies (e.g., anti-LAG-3 and / or anti-PD-1 antibodies) can be further combined with immunogenic agents, such as cancer cells, purified tumor antigens (including recombinant proteins, peptides, and carbohydrate molecules), and cells transfected with genes encoding immunostimulatory cytokines (He et al., (2004) J. Immunol. 173:4919-28). Non-limiting examples of tumor vaccines that can be used include tumor cells transfected to express peptides of melanoma antigens, such as peptides of gp100, MAGE antigens, Trp-2, MART1, and / or tyrosinase, or the cytokine GM-CSF. Other therapies that can be used in combination with anti-TROP2 antibodies include, but are not limited to, administration of interleukin-2 (IL-2), radiation, surgery, and hormone deprivation.

[0114] The therapeutic combinations discussed herein can be co-administered as a single composition in a pharmaceutically acceptable carrier, or as separate compositions with each agent in a pharmaceutically acceptable carrier, hi another embodiment, the therapeutic combinations can be administered sequentially.

[0115] Furthermore, when multiple doses of the combination therapy are administered sequentially, the order of sequential administration can be restored at each time of administration or maintained in the same order, sequential administration can be combined with simultaneous administration, or any combination thereof.

[0116] The present disclosure further provides a method for imaging TROP2-positive tissue, e.g., cancer tissue, in a subject in need thereof, comprising administering to the subject a radiolabeled anti-TROP2 antibody or antigen-binding portion thereof, immunoconjugate, or bispecific molecule of the present disclosure. The method is used to track / detect the distribution of tumors or cancers with high TROP2 expression, including, but not limited to, esophageal squamous cell carcinoma, colorectal cancer, pancreatic cancer, colon cancer, papillary thyroid cancer, breast cancer, bladder cancer, etc. In certain embodiments, the subject is a human.

[0117] Although the present invention and its advantages have been described in detail, it should be understood that various changes, substitutions, and alterations could be made therein without departing from the spirit and scope of the invention as defined in the appended claims.

[0118] The present disclosure is further illustrated by the following examples, which should not be construed as further limiting. The contents of all figures, and all references, Genbank sequences, patents and published patent applications cited throughout this application are hereby expressly incorporated by reference. [Example]

[0119] Example 1: Generation of single domain antibodies against TROP2 Library construction and screening Healthy adult camels were immunized according to the method described in E. Harlow and D. Lane, *Antibody: A Laboratory Manual*, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1998. The immunogen used was a recombinant human TROP2 protein (amino acid sequence set forth in SEQ ID NO: 15) containing a C-terminal human IgG1 Fc. The immunization dose consisted of 1.0 mg of human TROP2-Fc protein / camel / injection for the primary immunization and 0.5 mg of human TROP2-Fc protein / camel / injection for the boost immunization. To enhance the immune response, five immunizations were administered using complete Freud's adjuvant for the primary immunization and incomplete Freud's adjuvant (Sigma, St. Louis, MO) for the boost immunization. Lymphocytes were isolated from 100 ml of camel peripheral blood, and total RNA was extracted using the FastPure Cell / Tissue Total RNA Isolation Kit (Vazyme, Cat. #RC101). The extracted RNA was reverse transcribed into cDNA using the Hiscrit III 1st Strand cDNA Synthesis kit (+gDNA wiper) (Vazyme, Cat. #R312-01) according to the manufacturer's instructions. H The nucleic acid fragment encoding H was amplified by nested PCR.

[0120] Target V H The H nucleic acid fragment was cloned into the phage display vector pMECS using endonucleases PstI and NotI (NEB). The product was electrotransformed into E. coli competent cells TG1 (Lucigen Corporation), and a phage display library of single-domain antibodies against TROP2 was constructed and verified. By plating serial dilutions, the library capacity was determined to be approximately 2.0 × 10 8 To determine the insertion rate of the library, 95 clones were randomly selected and subjected to colony PCR. The insertion rate was found to be over 89.5%.

[0121] Discovery of single-domain antibodies against TROP2 Cross-reactivity of anti-TROP2 antibodies with human TROP2 protein was measured by phage ELISA using human TROP2-his protein (SEQ ID NO: 16, produced in-house). Phages that specifically bound to TROP2 were dissociated with glycine (pH 2.2, 100 mM) and infected log-phase E. coli TG1 to produce phages. Purified phages were used for the next round of screening. Similar screening was repeated two more times.

[0122] Selection of positive clones by phage enzyme-linked immunosorbent assay (ELISA) The TROP2-binding positive phages obtained from two rounds of panning were used to infect blank E. coli cells and plate them. A total of 940 single colonies were selected and inoculated into 2YT medium supplemented with 100 μg / mL ampicillin. When the bacterial solution reached an optical density (OD) of 0.6-0.8, 1 M IPTG (QIAGEN, Cat. #RT108-01) was added at a ratio of 1000:1, and antibody expression was induced overnight at 30°C.

[0123] ELISA plates were coated with 100 μl of 1 μg / ml human TROP2-his protein (produced in-house according to SEQ ID NO:16) or 1 μg / ml cynomolgus monkey TROP2-his protein (produced in-house according to SEQ ID NO:19) in carbonate / bicarbonate buffer (pH 9.6) overnight at 4°C, washed once with wash buffer (PBS + 0.05% v / v Tween®-20, PBST), and then blocked with 200 μl / well blocking buffer (5% w / v non-fat milk in PBST) for 2 hours at 37°C. Plates were washed four times and incubated with 100 μl each of bacterial culture supernatant and 200 ng / ml of sacituzumab (used as a benchmark, hereafter also referred to as BM or BM1, an in-house product made with heavy and light chain amino acid sequences set forth in SEQ ID NOs: 17 and 18) in 5% w / v nonfat milk in PBST for 40 minutes at 37° C. Plates were washed four times and incubated with THE® HA Tag Antibody [HRP], mAb, mouse antibody (diluted 1:5000 in PBST, GenScript, Cat#A 01296, 100 μl / well, for plates containing the disclosed bacterial culture supernatant) or Peroxidase AffiniPure F(ab´)2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (Jackson Immunoresearch, Cat#109-036-098, for plates containing the benchmark) for 40 minutes at 37° C. After a final wash, the plate was incubated at room temperature with 100 μl / well of ELISA substrate TMB (Innoreagents, Cat. #TMB-S-002). The reaction was stopped with 50 μl / well of 1 M H2SO4 for 3-10 minutes, and the absorbance of each well was read in a microplate reader in dual wavelength mode, with TMB at 450 nm and a reference wavelength of 630 nm. If the OD of the sample well was twice that of the blank well, the sample was considered positive. Exemplary supernatant results are shown in Table 2.

[0124] [Table 3]

[0125] Bacteria from positive wells were transferred to LB liquid medium supplemented with 100 μg / ml ampicillin and cultured, and the plasmid was extracted and subsequently sequenced.

[0126] The amino acid sequences of the antibodies produced by each clone were analyzed using the sequence alignment software Vector NTI, and the CDR and V sequences shown in Table 1 were finally determined. H Two single domain antibodies of the H sequence were obtained.

[0127] Example 2 Preliminary Evaluation of Single Domain Antibodies Against TROP2 V H Each vector containing the nucleotide encoding H was transiently transfected into 100 ml of 293F suspension cell cultures using 3 μg / ml PEI. The cell supernatant containing the single domain antibodies was harvested after 6 days in a shake flask, spun down to pellet the cells, and purified from the cell supernatant using a Protein A Sepharose column (Bestchrom (Shanghai) Biosciences, Cat# AA0273). Briefly, the column was washed with 5–10 column volumes of PBS buffer. The cell supernatant was passed through the column, and the column was washed with PBS buffer until the protein absorbance reached baseline. The column was eluted with elution buffer (0.1 M glycine-HCl, pH 2.7) and immediately collected in a 1.5 ml tube with neutralization buffer (1 M Tris-HCl, pH 9.0). Fractions containing the single domain antibodies were pooled and dialyzed overnight in PBS at 4°C.

[0128] The purified single domain antibodies were subjected to indirect ELISA, epitope binning, BIAcore affinity testing and cell-based internalization assays according to the protocols described below.

[0129] The single domain antibodies of the present disclosure were tested for cross-reactivity with cynomolgus monkey TROP2 protein in an indirect ELISA. Briefly, 96-well microplates were coated with 100 μl of 2 μg / ml human TROP2-his protein (prepared in-house using SEQ ID NO:16) in carbonate / bicarbonate buffer (pH 9.6) overnight at 4°C. The ELISA plates were washed once with wash buffer (PBS + 0.05% v / v Tween-20, PBST) and blocked with 200 μl / well of blocking buffer (5% w / v non-fat milk in PBST) for 2 hours at 37°C. The plates were washed four times and incubated with 100 μl / well of serially diluted anti-TROP2 antibodies of the present disclosure or controls (starting at 66.7 nM, 5-fold serial dilutions in 2.5% w / v non-fat milk in PBST) for 40 minutes at 37°C. The ELISA plate was washed four times again and incubated with Peroxidase AffiniPure F(ab´)2 Fragment Goat Anti-Human IgG, Fcγ Fragment Specific (Jackson Immuno Research, Cat#109-036-098, 1:5000 diluted in PBST buffer, 100 μl / well) for 40 minutes at 37°C. After the final wash, the plate was incubated with 100 μl / well TMB (Innoreagents) at room temperature. After 3–10 minutes, the reaction was stopped with 50 μl / well 1M H2SO4 at room temperature, and the absorbance of each well was read on a microplate reader in dual wavelength mode, with TMB at 450 nm and a reference wavelength of 630 nm. OD (450–630) values ​​were plotted against antibody concentration. Data were analyzed using Graphpad Prism software, and EC 50 The values ​​were reported and the results are shown in Figure 1.

[0130] Purified anti-TROP2 mouse monoclonal antibodies (mAbs) were characterized for binding affinity and binding kinetics using a Biacore T200 system (GE Healthcare, Pittsburgh, PA, USA). Briefly, goat anti-human IgG (GE Healthcare, Cat#BR100839, Human Antibody Capture Kit) was covalently coupled via primary amines to a CM5 chip (carboxymethyl dextran-coated chip from GE Healthcare, Cat#BR100530) using a standard amine coupling kit provided by Biacore (GE Healthcare, Pittsburgh, PA, USA). Unreacted sites on the chip (biosensor) surface were blocked with ethanolamine. The anti-TROP2 antibody of the present disclosure and a benchmark antibody at a concentration of 2 μg / ml were each flowed over the chip at a flow rate of 10 μL / min. Next, human TROP2-his protein (manufactured in-house with SEQ ID NO:16) or cynomolgus monkey TROP2-his protein (manufactured in-house with SEQ ID NO:19), diluted 2-fold from 160 nM with HBS-EP+ buffer (provided by Biacore), was flowed over the chip at a flow rate of 30 μL / min. Antigen-antibody binding kinetics were monitored for 2 minutes, and dissociation kinetics were monitored for 10 minutes. Association and dissociation curves were fitted to a 1:1 Langmuir binding model using BIAcore evaluation software. The results are shown in Table 3.

[0131] Anti-TROP2 antibodies were tested for epitope binding in a competitive ELISA assay. Briefly, 100 μl of Benchmark at 1 μg / mL in PBS was coated onto a 96-well microplate for 2 hours at 37°C. After washing the ELISA plate once with wash buffer (PBS + 0.05% v / v Tween-20, PBST), it was blocked with 200 μl of blocking buffer (5% w / v non-fat milk in PBST) for 2 hours at 37°C. While blocking, an anti-TROP2 antibody or control of the present disclosure was diluted in a 5-fold serial dilution starting at 80 nM with biotin-labeled human TROP2-his protein (SEQ ID NO: 16, 2.5% w / v non-fat milk in PBST) and incubated for 40 minutes at room temperature. After washing the plate four times, 100 μl of the antibody / TROP2-his protein mixture was added per well to the Benchmark-coated plate. After incubation at 37°C for 40 minutes, the plate was washed again four times with wash buffer. Next, the plate was added with 100 μl of Peroxidase Streptavidin (1:10,000 dilution in PBST buffer, Jackson Immunoresearch, Cat#016-030-084) and incubated at 37°C for 40 minutes. The plate was washed again with wash buffer. Finally, TMB was added, and the reaction was stopped with 1 M H2SO4. The absorbance of each well was read using a microplate reader in dual wavelength mode, with TMB at 450 nm and a reference wavelength of 630 nm, and the OD (450-630) values ​​were plotted against the antibody concentration. Data were analyzed using Graphpad Prism software, and IC 50 The values ​​were reported and the results are shown in Figure 2.

[0132] In a cell-based internalization assay, the internalization efficiency of anti-TROP2 antibodies was precisely evaluated using Biosion 293F-TROP2 cells (clone ID #3A8), which stably express full-length human TROP2 (uniprot #P09758, SEQ ID NO:20) on the cell membrane. 293F-TROP2 cells were generated by transfecting 293F cells (Thermofisher Inc., Cat #11625019) with the pCMV-TP plasmid, which contained the TROP2 coding sequence inserted between the EcoRI and XbaI sites, according to the instructions for Lipofectamine 3000 transfection reagent (Thermo Fisher). First, 1.5 x 10 ng / ml of 293F-TROP2 antibody was transfected into 100 μL of FreeStyle 293 medium (Gibco, Cat #12338-018) supplemented with 10% v / v FBS (Gibco, Cat #10099-141). 3 293F-TROP2 cells were plated in a 96-well flat-bottom plate (Thermo Fisher Scientific Inc., Cat. #167008). The day after cell seeding, the disclosed or control anti-TROP2 antibody (1.6 μg / mL in 10% v / v FBS FreeStyle 293 medium) was mixed with DTTP1170, a recombinant protein synthesized using the amino acid sequence set forth in SEQ ID NO:22, at a volumetric ratio of 1:1 at 1.6 μg / mL in 10% v / v FBS FreeStyle 293 medium. After incubation at room temperature for 30 minutes, serial 3-fold dilutions were made in cell culture medium, starting from 0.8 μg / mL. Next, 100 μl of the serially diluted antibody / DTTP1170 mixture was added to the cell plate and incubated at 37°C in a CO2 incubator for 72 hours. Cell Titer Glo reagent (Vazyme Biotech Co., Ltd., Cat# DD1101-02) was added to the plate and incubated at room temperature for 3-5 minutes. The cell culture plate was then analyzed using a Tecan Infinite 200Pro plate reader. Data were analyzed using Graphpad Prism software, and IC values ​​were calculated. 50The values ​​were reported as the antibody concentration that achieved 50% of the maximum inhibition of cell viability. The results are shown in Figure 3. When the mAb-DTTP conjugate was internalized into target cells, target cell viability was significantly reduced. When the conjugate was not internalized, free DTTP1170 in the culture medium had little or no cell-killing activity.

[0133] [Table 4]

[0134] Table 3 shows that the single domain antibodies of the present disclosure specifically bind to human TROP2 with higher binding affinity than the benchmark and specifically bind to cynomolgus monkey TROP2 with affinity comparable to the benchmark.

[0135] FIG. 1 shows that the single domain antibodies of the present disclosure specifically bind to human TROP2 protein with similar Bmaxs but slightly lower EC50 values ​​compared to the benchmark.

[0136] As shown in Figure 2, the single domain antibodies of the present disclosure were able to inhibit human TROP2-benchmark binding, suggesting that they bind to the same or similar epitope as benchmark.

[0137] Furthermore, as shown in Figure 3, the DT3C conjugates of the single domain antibodies of the present invention caused target cell death more efficiently than the benchmark DT3C conjugates.

[0138] Example 3 Genetic Engineering of Single Domain Antibody 01-9F 01-9F Single Domain Antibody (V H H) was cloned in frame into the human IgG1 Fc region (SEQ ID NO:14, produced in-house), where V H The C-terminus of H was linked to the N-terminus of the Fc region.

[0139] V linked to human IgG1-Fc region HEach vector containing nucleotides encoding H was transiently incubated with 100 ml 293F suspension cell culture with 3 μg / ml PEI. H The cell supernatant containing the heavy chain-only antibody (H-Fc) is harvested after 6 days in shake flasks, spun to pellet the cells, and the resulting heavy chain-only antibody (also referred to herein as 01-9F-Fc) is purified from the cell supernatant as described above.

[0140] For example, the single domain antibody 01-9F may be further modified in the CDR2 or CDR3 regions to avoid or reduce post-translational modifications such as isomerization of specific amino acid residues in the CDR regions, which may adversely affect antibody production, stability, safety and / or efficacy. H A total of 11 modified variants, 01-9F-CDR-V1 to 01-9F-CDR-V11, whose sequence ID numbers are listed in Table 1, were obtained.

[0141] Any of V1 to V11 linked to the human IgG1 heavy chain constant region (SEQ ID NO: 14) H Each vector containing nucleotides encoding H was transiently transfected into 100 ml 293F suspension cell cultures with 3 μg / ml PEI.

[0142] Example 4 Characterization of the 01-9F-Fc variant Cell supernatants containing heavy chain-only antibodies (01-9F-Fc variants), i.e., 01-9F-CDR-V1-Fc through 01-9F-CDR-V11-Fc, were harvested after 6 days in shake flasks, spun down to pellet cells, and tested in BIAcore affinity studies and cell-based internalization assays according to the protocols in the examples above, with the modifications described below.

[0143] In the BIAcore test, cell supernatants containing the 01-9F-Fc variants were flowed over the chip at a flow rate of 10 μL / min instead of purified anti-TROP2 antibodies, and 40 nM human TROP2-his protein (prepared in-house according to SEQ ID NO: 16) in HBS-EP+ buffer (provided by Biacore) was flowed over the chip at a flow rate of 30 μL / min instead of serially diluted human TROP2-his protein. D ,K a and K. d The values ​​were determined and are summarized in Table 4 below.

[0144] For cell-based internalization assays, DT3C was used to bind heavy chain-only antibodies, and an in-house anti-CD22 antibody was used as a negative control. Briefly, 1.5 × 10 mAbs were added to 100 μL of FreeStyle 293 medium (Gibco, Cat# 12338-018) supplemented with 10% v / v FBS (Gibco, Cat# 10099-141). 3 293F-TROP2 cells were plated in a 96-well flat-bottom plate (Thermo Fisher Scientific Inc., Cat#167008). The 01-9F-Fc mutant or control was mixed with 40 nM DT3C protein in a 1:1 volumetric ratio in FreeStyle 293 medium supplemented with 10% v / v FBS and incubated at room temperature for 30 minutes. The mixture was then serially diluted 3-fold starting from 20 nM in cell culture medium. The results are shown in Figure 4.

[0145] [Table 5]

[0146] Table 4 shows that the 01-9F-CDR-Fc variant of the present disclosure specifically binds to human TROP2 with comparable binding affinity compared to 01-9F and 01-9F-Fc.

[0147] As shown in Figure 4, the chimeric antibody E1A9C8A7 was able to restore luciferase expression downregulated by PD-1-PD-L1 interaction in GS-J2 / PD-1 cells more efficiently than nivolumab but with activity comparable to that of pembrolizumab. DT3C conjugates of 01-9F-Fc variants, including 01-9F-CDR-V5-Fc, 01-9F-CDR-V9-Fc, and 01-9F-CDR-V11-Fc, induced target cell death more efficiently than the benchmark DT3C conjugate.

[0148] Example 5 Humanization of 01-9F-CDR-V11 The mutant 01-9F-CDR-V11-Fc was purified and humanized, and V H A total of 24 sample humanized antibodies were obtained, with sequence ID numbers 01-9F-CDR-V11-V1-Fc to 01-9F-CDR-V11-V24-Fc in Table 1.

[0149] Any V of 01-9F-CDR-V11-V1 to 01-9F-CDR-V11-V24 linked to a human IgG1 heavy chain constant region (SEQ ID NO: 14) H Vectors containing nucleotides encoding H were each transiently transfected into 100 ml 293F suspension cell cultures at 3 μg / ml PEI.

[0150] Example 6 Characterization of an Exemplary Humanized 01-9F-CDR-V11 Antibody Cell supernatant containing the humanized 01-9F-CDR-V11 antibody was harvested after 6 days in shake flasks, spun down to pellet the cells, and tested for binding affinity to human TROP2 using a BiAcore T200 system (GE Healthcare, Pittsburgh, PA, USA) using the protocol described in the previous example with the following modifications.

[0151] In the BIAcore test, cell supernatants containing the humanized 01-9F-CDR-V11 antibody were flowed over the chip at a flow rate of 10 μL / min, and 40 nM human TROP2-his protein (SEQ ID NO: 16, produced in-house) in HBS-EP+ buffer (provided by Biacore) was flowed over the chip at a flow rate of 30 μL / min. D ,K a and K. d The values ​​were determined and are summarized in Table 5 below.

[0152] [Table 6]

[0153] Table 5 shows that the humanized 01-9F-CDR-V11 antibodies have high human TROP2 binding affinity, with 01-9F-CDR-V11-V1, 01-9F-CDR-V11-V9 and 01-9F-CDR-V11-V11 showing the highest binding affinity.

[0154] Example 7 Further Characterization of the Exemplary Humanized 01-9F-CDR-V11 Antibody Humanized antibodies 01-9F-CDR-V11-V1-Fc, 01-9F-CDR-V11-V9-Fc and 01-9F-CDR-V11-V11-Fc were purified as described above and tested in a cell-based internalization assay according to the protocol in the previous example, with the modifications described below.

[0155] For cell-based internalization assays, DT3C was used to bind these antibodies, and an in-house anti-CD22 antibody was used as a negative control. Briefly, 1.5 x 10 mAbs were mixed in 100 μL FreeStyle 293 medium (Gibco, Cat# 12338-018) supplemented with 10% v / v FBS (Gibco, Cat# 10099-141). 3293F-TROP2 cells (clone ID#3A8) were plated in a 96-well flat-bottom plate (Thermo Fisher Scientific Inc., Cat#167008). Humanized antibodies or controls were mixed with 40 nM DT3C protein in a 1:1 volumetric ratio in FreeStyle 293 medium supplemented with 10% v / v FBS and incubated at room temperature for 30 minutes. The mixture was then serially diluted 3-fold starting from 20 nM in cell culture medium. The results are shown in Figure 5.

[0156] As shown in Figure 5, DT3C conjugates of humanized 01-9F-CDR-V11 antibody, including 01-9F-CDR-V11-V1-Fc, 01-9F-CDR-V11-V9-Fc, and 01-9F-CDR-V11-V11-Fc, caused target cell death at similar rates to the benchmark DT3C conjugate.

[0157] Humanized antibody 01-9F-CDR-V11-V11-Fc was further tested in Biacore, capture ELISA, indirect ELISA, cell-based binding FACS, competitive ELISA and protein thermal shift assays according to the protocols described below and in the preceding examples with or without modifications.

[0158] The BIAcore test results are summarized in Table 6 below.

[0159] For capture ELISA, 96-well plates were coated with 100 μl of 2 μg / ml AffiniPure F(ab´)2 Fragment Goat Anti-Human IgG, Fcγ fragment specific (Jackson Immuno Research, Cat#109-006-008) in PBS overnight at 4° C. Plates were washed once with wash buffer (PBS + 0.05% v / v Tween-20, PBST) and blocked with 200 μl / well of blocking buffer (5% w / v non-fat milk in PBST) for 2 hours at 37° C. Plates were washed four times and incubated with 100 μl of serially diluted anti-TROP2 antibodies of the present disclosure, benchmark, or negative control hIgG (Human Immunoglobulin for Intravenous Injection, pH 4, Hualan Biological Engineering Inc.) (5-fold diluted in 2.5% w / v nonfat milk in PBST, starting at 66.7 nM) for 40 minutes at 37°C, followed by another four washes. Plates containing captured anti-TROP2 antibodies were incubated with biotin-labeled human TROP2-his protein (in-house production, SEQ ID NO: 16, 56.7 ng / mL in 2.5% w / v nonfat milk in PBST, 100 μl / well) for 40 minutes at 37°C. After washing four times, the plates were incubated with streptavidin-conjugated HRP (1:10,000 diluted in PBST buffer, Jackson Immuno Research, Cat. #016-030-084, 100 μl / well) for 40 minutes at 37°C. After the final wash, the plates were incubated with 100 μl / well of ELISA substrate TMB (Innoreagents, Cat. #TMB-S-002) at room temperature. The reaction was stopped at room temperature for 3-10 minutes with 50 μl / well of 1 M H2SO4. The absorbance of each well was read using a microplate reader in dual wavelength mode, with TMB at 450 nm and a reference wavelength of 630 nm. OD (450-630) values ​​were plotted against antibody concentration. Data were analyzed using Graphpad Prism software, and EC 50 The values ​​were reported and the results are shown in Figure 6.

[0160] For indirect ELISA, 100 μl / well of AffiniPure goat anti-human IgG, Fcγ fragment specific (Jackson Immunoresearch, Cat#109-005-098) was used. The results are shown in Figures 7 and 8.

[0161] For cell-based coupled FACS, 293F-TROP2 cells were harvested from cell culture flasks, washed twice, and resuspended in phosphate-buffered saline (PBS) containing 2% v / v fetal bovine serum (FACS buffer). Then, 2 × 10 cells were plated per well. 5 293F-TROP2 cells were incubated in a 96-well plate with 100 μl of anti-TROP2 antibody or control at various concentrations (starting from 66.7 nM, 4-fold serial dilutions in FACS buffer) for 40 minutes on ice. Cells were washed twice with FACS buffer, and 100 μL / well of R-Phycoerythrin AffiniPure goat anti-human IgG, Fcγ fragment specific (1:1000 dilution in FACS buffer, Jackson Immunoresearch, Cat#109-115-098) was added. After 40 minutes of incubation at 4°C in the dark, cells were washed twice and resuspended in FACS buffer. Fluorescence was measured using a Becton Dickinson FACS Canto II-HTS instrument, and MFI (mean fluorescence intensity) was plotted against antibody concentration. Data were analyzed using Graphpad Prism software, and EC 50 The values ​​were reported and the results are shown in Figure 9.

[0162] For epitope binning, a benchmark concentration of 2 μg / mL was used, with 100 μL / well. Humanized antibody 01-9F-CDR-V11-V11 or a control was diluted with biotin-labeled human TROP2-his protein (SEQ ID NO: 16, 8.7 ng / mL in 2.5% w / v non-fatty milk in PBST) in a 5-fold serial dilution starting from 66.7 nM and incubated at room temperature for 40 minutes. The results are shown in Figure 10.

[0163] For thermal shift assays, Tm (melting temperature) was measured using the Protein Thermal Shift™ Dye Kit (Thermo Fisher, Cat# 4461146). Briefly, GloMelt® dye was thawed and allowed to warm to room temperature. The vial containing the dye was vortexed and centrifuged. Next, 5 μL of a 200x diluted dye was added to 95 μL PBS to prepare a 10x diluted dye. 2 μL of the 10x diluted dye was added to 10 μg of humanized antibody, and PBS was added to bring the total reaction volume to 20 μL. The tube containing the dye and antibody was briefly spun and placed in a CFX Connect Real-Time PCR Detection System (Bio-Rad, Cat# 1855201). The results are shown in Figures 11A-11C.

[0164] [Table 7]

[0165] Table 6 shows that 01-9F-CDR-V11-V11-Fc exhibited comparable binding affinity to human and cynomolgus monkey TROP2 compared with 01-9F and 01-9F-CDR-V11-Fc, and slightly higher than the benchmark. Figures 6-9 show that 01-9F-CDR-V11-V11-Fc has higher binding activity to human and monkey TROP2 than the benchmark.

[0166] As shown in Figure 10, 01-9F-Fc-CDRV11-V11 was able to inhibit the binding of BM1-TROP2, indicating that it may bind to the same epitope as BM1.

[0167] Furthermore, as shown in Figures 11A-11C, based on the melting temperatures, antibodies 01-9F, 01-9F-CDR-V11-Fc, and 01-9F-CDR-V11-V11-Fc were likely stable in the human body.

[0168] While the present disclosure has been described above in connection with one or more embodiments, it should be understood that the disclosure is not limited to those embodiments, and that the present description is intended to cover all alternatives, modifications, and equivalents that may be included within the spirit and scope of the appended claims. All references cited herein are further incorporated by reference in their entirety.

[0169] The sequences in this application are summarized below. [Table 8]

[0170] [Table 9]

[0171] [Table 10]

[0172] [Table 11]

[0173] [Table 12]

[0174] [Table 13]

[0175] [Table 14]

[0176] [Table 15]

[0177] [Table 16]

[0178] Thus, while preferred embodiments of the invention have been described in detail, it will be understood that the invention defined by the above paragraphs is not limited to the specific details set forth in the above description, as many obvious variations thereof are possible without departing from the spirit or scope of the invention.

Claims

1. 1. A heavy chain-only antibody or antigen-binding portion thereof capable of binding to TROP2, comprising a variable region including a CDR1 region, a CDR2 region, and a CDR3 region, wherein the CDR1 region, the CDR2 region, and the CDR3 region comprise the amino acid sequences set forth below: (1) SEQ ID NOs: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and G, respectively; (2) SEQ ID NOs: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are E and G, respectively; (3) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and A, respectively; (4) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are I and G, respectively; (5) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are E, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are E and G, respectively; (6) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, A, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are E and G, respectively; (7) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are E, G, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and A, respectively; (8) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, A, D, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and A, respectively; (9) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are E, G, E, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are E and G, respectively; (10) SEQ ID NO: 1, 2, and 3, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, A, E, and S, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are E and G, respectively; (11) SEQ ID NO: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are E, G, D, and T, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and A, respectively; or (12) SEQ ID NOs: 1, 2, and 3, respectively, wherein the 5th, 6th, 12th, and 13th amino acid residues of SEQ ID NO: 2 are D, A, D, and T, respectively, and the 10th and 11th amino acid residues of SEQ ID NO: 3 are D and A, respectively.

2. 2. The heavy chain-only antibody or antigen-binding portion thereof of claim 1, wherein the variable region comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13: wherein the amino acid residues at positions 11, 13, 107, 108, and 124 of SEQ ID NO: 4 are S, Q, D, G, and P, respectively; S, Q, E, G, and P, respectively; S, Q, D, A, and P, respectively; S, Q, I, G, and P, respectively; or T, G, D, G, and L, respectively; the 54th, 55th, and 61st amino acid residues of SEQ ID NO: 5 are E, G, and D, respectively; D, A, and D, respectively; E, G, and E, respectively; or D, A, and E, respectively; the amino acid residues at positions 54, 55, and 62 of SEQ ID NO: 6 are E, G, and S, respectively; D, A, and S, respectively; E, G, and T, respectively; or D, A, and T, respectively; the amino acid residues at positions 27, 29, 71 and 97 of SEQ ID NO: 8 are F, Y, K and A, respectively; L, F, K and A, respectively; L, Y, R and A, respectively; L, Y, K and R, respectively; or L, Y, K and A, respectively; the amino acid residues at positions 27, 29, 71 and 97 of SEQ ID NO: 9 are F, Y, K and A, respectively; L, F, K and A, respectively; L, Y, R and A, respectively; L, Y, K and R, respectively; or L, Y, K and A, respectively; the amino acid residues at positions 27, 29, 71 and 97 of SEQ ID NO: 10 are F, Y, K and A, respectively; L, F, K and A, respectively; L, Y, R and A, respectively; L, Y, K and R, respectively; or L, Y, K and A, respectively; the amino acid residues at positions 27, 29, 71 and 97 of SEQ ID NO: 11 are F, Y, K and A, respectively; L, F, K and A, respectively; L, Y, R and A, respectively; L, Y, K and R, respectively; or L, Y, K and A, respectively; The 37th and 47th amino acid residues of SEQ ID NO: 13 are V and W, respectively; or F and G, respectively.

3. 3. The heavy chain-only antibody or antigen-binding portion thereof of claim 1 or 2, comprising a constant region having the amino acid sequence of SEQ ID NO: 14 attached to said variable region.

4. a) capable of binding to human TROP2; (b) capable of binding to monkey TROP2; and / or (c) TROP2 ＋ 4. The heavy chain-only antibody or antigen-binding portion thereof of any one of claims 1 to 3, which is capable of being internalized by a cell.

5. 5. The heavy chain-only antibody or antigen-binding portion thereof of any one of claims 1 to 4, which is a camelid, chimeric or humanized antibody.

6. An immunoconjugate comprising the heavy chain-only antibody or antigen-binding portion thereof of any one of claims 1 to 5, conjugated to a toxin or a radioisotope.

7. The immunoconjugate of claim 6, wherein the toxin is a recombinant protein comprising the amino acid sequence of SEQ ID NO:

22.

8. A nucleic acid molecule encoding the heavy chain-only antibody or antigen-binding portion thereof of any one of claims 1 to 5.

9. An expression vector comprising the nucleic acid molecule of claim 8.

10. A host cell comprising the expression vector of claim 9.

11. A pharmaceutical composition comprising the heavy chain-only antibody or antigen-binding portion thereof according to any one of claims 1 to 5, or the immune complex according to claim 6 or 7, the nucleic acid molecule according to claim 8, the expression vector according to claim 9, or the host cell according to claim 10, and a pharmaceutically acceptable carrier.

12. 12. The pharmaceutical composition of claim 11, further comprising an anti-tumor agent.

13. 13. The pharmaceutical composition of claim 11 or 12 for use in the treatment of a disease associated with TROP2.

14. 14. The pharmaceutical composition of claim 13, wherein the disease is a cancer selected from the group consisting of breast cancer, colorectal cancer, gastric adenocarcinoma, esophageal cancer, hepatocellular carcinoma, non-small cell lung cancer, small cell lung cancer, ovarian epithelial cancer, prostate cancer, pancreatic ductal adenocarcinoma, head and neck cancer, squamous cell carcinoma, renal cell carcinoma, bladder tumor, cervical cancer, endometrial cancer, follicular thyroid cancer, and glioblastoma multiforme.

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