Novel Saccharomyces cerevisiae strains and their uses

Abstract

The present invention relates to a Saccharomyces cerevisiae strain deposited under accession number KCTC14268BP; a cosmetic composition comprising at least one selected from the group consisting of the strain, a culture of the strain, and a fermentation product of the strain; a quasi-drug composition; or a food composition; a skin improvement method using the strain, a culture of the strain, or a fermentation product of the strain; and a skin improvement use using the strain, a culture of the strain, or a fermentation product of the strain.

Description

Technical Field

[0005]

[0001] The present invention relates to a Saccharomyces cerevisiae strain deposited under the accession number KCTC 14268BP; a cosmetic composition, a quasi-drug composition, or a food composition comprising any one or more selected from the group consisting of the strain, a culture of the strain, and a fermented product of the strain; a method for improving skin using the strain, the culture of the strain, and the fermented product of the strain; and use of the strain, the culture of the strain, and the fermented product of the strain for improving skin.

Background Art

[0002] Some components of cosmetics may cause various problems such as inflammation, acne, and swelling on the skin (Non-Patent Document 1). In addition, various substances used as raw materials for functional cosmetics may also have problems of low safety or subtle effects on the skin.

[0003] As an example, retinol, adenosine, animal placenta-derived protein, and chlorella extract are known as cosmetics for enhancing skin elasticity and improving wrinkles. The most widely known retinol has limitations in the amount of use due to safety problems such as irritation during skin application, and chlorella extract has little effect, making it difficult to expect substantial effects on skin elasticity and wrinkle improvement.

[0004] As another example, generally known whitening components include kojic acid, arbutin, hydroquinone, vitamin C or their derivatives, and various plant extracts. However, these have problems such as limitations in the amount of use due to safety problems such as irritation and redness during skin application, or little effect and inability to expect substantial effects.

Prior Art Documents

Non-Patent Documents

[0005] [Non-Patent Document 1] Maibach. HI, Contact Dermatitis, 6. 369-404, 1980 [Overview of the Initiative] [Problems that the invention aims to solve]

[0006] The inventors of this invention conducted research on raw materials from natural resources that are safer and have superior skin-improving effects, and confirmed that Saccharomyces cerevisiae has skin-improving effects, and that a newly discovered strain of Saccharomyces cerevisiae from the flowers of Dendrobium nobile exhibits significantly superior skin-improving effects compared to other Saccharomyces cerevisiae strains, thereby completing the present invention. [Means for solving the problem]

[0007] One object of the present invention is to provide a strain of Saccharomyces cerevisiae deposited under deposit number KCTC14268BP.

[0008] Another object of the present invention is to provide a cosmetic composition for improving skin containing one or more of the aforementioned bacterial strain, a culture of the bacterial strain, and a fermented product of the bacterial strain.

[0009] Another object of the present invention is to provide a quasi-drug composition for improving skin, comprising one or more of the aforementioned bacterial strain, a culture of the bacterial strain, and a fermented product of the bacterial strain.

[0010] Another object of the present invention is to provide a food composition for improving skin, comprising one or more of the above-mentioned strain, a culture of the strain, and a fermented product of the strain.

[0011] Another object of the present invention is to provide a method for improving skin using one or more of the above-mentioned bacterial strain, cultures of the bacterial strain, and fermented products of the bacterial strain.

[0012] Another object of the present invention is to provide one or more of the aforementioned bacterial strains, cultures of the bacterial strains, and fermented products of the bacterial strains for skin improvement. [Effects of the Invention]

[0013] The Saccharomyces cerevisiae strain, culture of the strain, or fermented product of the strain of the present invention have excellent skin-improving effects and are therefore highly suitable for use as cosmetic compositions and quasi-drug compositions for skin improvement. This opens up possibilities for various industrial applications in cosmetics and quasi-drugs. [Modes for carrying out the invention]

[0014] The details of the present invention will be described below. On the other hand, each description and embodiment disclosed in the present invention can be applied to other descriptions and embodiments with respect to common matters. Furthermore, all combinations of the various elements disclosed in the present invention belong to the category of this application. Moreover, the category of this application cannot be considered to be limited by the specific descriptions described below.

[0015] One aspect of this application provides a strain of Saccharomyces cerevisiae deposited under deposit number KCTC14268BP.

[0016] In this invention, "Saccharomyces cerevisiae" refers to a species of yeast belonging to the Saccharomycetaceae family and the genus Saccharomyces. This species has been widely used since ancient times, playing an important role in winemaking, breadmaking, and brewing. The characteristics of Saccharomyces cerevisiae vary depending on the growing region and natural environmental conditions, and the strain and its fermentation activity may change. The Saccharomyces cerevisiae of this invention may be derived from Dendrobium candidum, but is not limited thereto.

[0017] In this invention, "Dendrobium candidum" is one of the Orchidaceae plants widely known as a traditional medicinal plant. The stems of Dendrobium candidum have been widely used for medicinal purposes since ancient times. They are cylindrical, 15-50 cm long, 0.15-0.3 cm in diameter, with internodes of 1-4 cm, and have a yellow outer surface with a glossy, longitudinally wrinkled lower section. Above the nodes are the remnants of the pedicel of the inflorescence and the remaining leaf sheath, and the opening of the leaf sheath is flared. The Dendrobium candidum used in this invention may be purchased from a commercially available source, or it may be collected or cultivated in the wild.

[0018] The Saccharomyces cerevisiae of the present invention may be derived from Dendrobium moniliforme, and Dendrobium moniliforme may be used by specifically selecting the flower part from among the plant parts, and the Saccharomyces cerevisiae may be derived from the flowers of Dendrobium moniliforme, but there are no restrictions on the part, origin, and method of obtaining it.

[0019] Specifically, in the examples of the present invention, it was confirmed that the fermented product of Saccharomyces cerevisiae (KCTC14268BP) derived from Dendrobium nobile had superior skin-improving effects compared to the fermented product of Saccharomyces cerevisiae (KCTC 7296), a common yeast.

[0020] Another aspect of the present invention provides a cosmetic composition for skin improvement comprising one or more of the following: Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, a culture of the strain, and a ferment of the strain.

[0021] The culture of the present invention means a culture solution, a concentrated culture solution, a dried product of the culture solution, a culture filtrate, a concentrated culture filtrate, or a dried product of the culture filtrate obtained by culturing a specific microorganism in a culture medium. The culture solution means containing a specific bacterium, and the culture filtrate means substantially not containing a specific bacterium (here, substantially means excluding a specific bacterium separated by filtration or the like, and does not mean that the bacterium is completely excluded from the filtrate). The dosage form of the culture is not limited, and as an example, it may be liquid, emulsion, or solid.

[0022] In the present invention, the term "fermentation" means that the process in which a microorganism decomposes an organic substance using the enzymes it itself has is not a spoilage reaction. The fermentation reaction and the spoilage reaction proceed in a similar process. If useful substances are produced as a result of decomposition, it is called fermentation, and if a bad smell appears or harmful substances are produced, it is called spoilage.

[0023] In the present invention, the method for obtaining a fermented product from the strain is not particularly limited and can be obtained according to methods commonly used in the technical field or similar fields.

[0024] In the present invention, the fermented product obtained from the strain includes not only the fermented substance itself, but also the culture medium of the strain and the coexisting strain of the culture, the fermented product obtained by filtering the strain from the culture medium, the fermented product obtained by sterilizing the strain from the culture medium and then filtering it, the extract obtained by extracting the fermented product or the culture medium containing it, the dilution obtained by diluting the fermented product or its extract, the concentrate, the dried product obtained by drying the fermented product or its extract, the lysate obtained by collecting and crushing the cells of the strain, and all kinds of substances containing the fermented product generated from the strain.

[0025] In one specific example, the skin improvement may be any one or more selected from the group consisting of skin moisturization, wrinkle improvement, promotion of hyaluronic acid production, promotion of collagen synthesis, enhancement of elasticity, and prevention of aging, but is not limited thereto.

[0026] In the present invention, "improvement" means all actions that reduce the severity of a condition, such as alleviating or treating a condition, and can include all or more of the following: skin moisturizing, wrinkle improvement, promotion of hyaluronic acid production, promotion of collagen synthesis, increased elasticity, and anti-aging.

[0027] Specifically, the inventors confirmed that the fermented product of Saccharomyces cerevisiae KCTC14268BP has superior skin-improving effects compared to the fermented product of the general yeast Saccharomyces cerevisiae KCTC 7296. More specifically, the inventors confirmed that the fermented product of Saccharomyces cerevisiae KCTC14268BP has superior effects in promoting collagen synthesis and hyaluronic acid synthesis. Therefore, it was confirmed that Saccharomyces cerevisiae KCTC14268BP derived from Dendrobium nobile, its culture, or its fermented product are superior to general yeast, its culture, or its fermented product in skin-improving effects such as skin moisturizing, wrinkle improvement, elasticity enhancement, hyaluronic acid production promotion, collagen synthesis promotion, and anti-aging.

[0028] In this invention, "skin moisturizing" refers to all actions that supply moisture to the skin, block moisture evaporation to maintain skin flexibility, and induce uniform exfoliation to maintain a smooth surface, while "dryness" refers to a state in which such skin moisturizing is insufficient. Specifically, skin moisturizing may be achieved by promoting hyaluronic acid production, but is not limited to this.

[0029] In this invention, "hyaluronic acid" refers to a type of glycosaminoglycan, which is a chain-like high-molecular-weight polysaccharide substance in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked together.

[0030] In this invention, "wrinkles" can be broadly divided into expression wrinkles caused by facial muscles used to create facial expressions and fine wrinkles that result from general or localized skin deterioration. Specifically, wrinkles can be induced by genetic causes, a decrease in collagen and elastic fibers in the dermis, and the external environment. For the purposes of this invention, the term "wrinkles" can be used to include, but is not limited to, expression wrinkles or fine wrinkles.

[0031] In this invention, "aging" refers to the phenomenon of a decline in biological structure and function over time, and the aging prevention of this invention is not limited to the degree of improvement of aging, such as preventing, suppressing, or delaying aging. The wrinkle improvement and aging prevention in this invention may be achieved by promoting collagen synthesis, but is not limited to this.

[0032] The cosmetic composition of the present invention may be, but is not limited to, a dosage form selected from the group consisting of solutions, topical ointments, creams, foams, nourishing lotions, softening lotions, perfumes, packs, softening waters, emulsions, makeup bases, essences, soaps, liquid cleansers, bath additives, sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches, and sprays.

[0033] The cosmetic composition of the present invention may further contain one or more cosmetically acceptable carriers commonly used in skin cosmetics, and may, but is not limited to, oils, water, surfactants, humectants, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., as usual ingredients.

[0034] The cosmetically acceptable carriers included in the cosmetic composition of the present invention vary depending on the dosage form of the cosmetic composition.

[0035] When the cosmetic formulation of the present invention is an ointment, paste, cream, or gel, animal oils, vegetable oils, waxes, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc., may be used as carrier components, but are not limited thereto. These may be used alone or in combination of two or more.

[0036] When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer, or emulsifier may be used as the carrier component. For example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, etc. may be used. In particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil, and sesame oil, glycerol aliphatic esters, polyethylene glycol, or sorbitan fatty acid esters may be used, but are not limited thereto. These may be used alone or in combination of two or more.

[0037] When the dosage form of the present invention is a suspension, the carrier component may be, but is not limited to, water, ethanol, liquid diluents such as propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. These may be used alone or in combination of two or more.

[0038] When the dosage form of the present invention is soap, the carrier component may be, but is not limited to, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerin, sugars, etc. These may be used alone or in combination of two or more.

[0039] When the dosage form of the present invention is a powder or a spray, the carrier component may be lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or a mixture thereof, and in particular, in the case of a spray, it may further contain a propellant such as hydrochlorofluorocarbon, propane / butane, or dimethyl ether.

[0040] On the other hand, all components described in the present invention may preferably be included in the composition of the present invention within a range that does not exceed the maximum usage values ​​specified in the regulations concerning cosmetic safety standards and the Chinese "Cosmetic Safety and Technical Specifications."

[0041] Another aspect of the present invention provides a quasi-drug composition for skin improvement comprising one or more of the following: Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, a culture of the strain, and a fermented product of the strain.

[0042] The aforementioned Saccharomyces cerevisiae, cultures, ferments, and skin improvement are as described above.

[0043] In the present invention, the term "quasi-drug" may be selected from the group consisting of body cleansers, disinfectants, detergents, kitchen detergents, cleaning agents, toothpaste, gargles, wet wipes, detergents, soaps, hand washes, hair washes, hair softeners, humidifier fillers, masks, ointments, and filter fillers, but is not limited thereto.

[0044] The quasi-drug composition of the present invention may further contain, as necessary, pharmaceutically acceptable carriers, excipients, or diluents in addition to the above-mentioned components. The pharmaceutically acceptable carriers, excipients, or diluents are not limited as long as they do not impair the effects of the present invention, and examples include fillers, bulking agents, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, fragrances, and preservatives.

[0045] Typical examples of pharmaceutically acceptable carriers, excipients, or diluents of the present invention include lactose, glucose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia gum, alginate, calcium phosphate, calcium carbonate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil, injectable esters, vitepsol, macrogol, Twin 61, cocoa butter, lauric acid butter, and the like.

[0046] When the bacterial strain, culture thereof, or fermented product thereof of the present invention is used as a quasi-drug, it may further contain one or more active ingredients exhibiting the same or similar functions. For example, it may contain known ingredients that promote beneficial skin bacteria, suppress harmful skin bacteria, soothe skin, improve skin inflammation, whiten skin, regenerate skin, heal wounds, moisturize skin, prevent aging, and enhance elasticity. By including additional wrinkle-improving, skin-moisturizing, anti-aging, and elasticity-enhancing ingredients, the wrinkle-improving, skin-moisturizing, anti-aging, and elasticity-enhancing effects of the composition of the present invention can be further enhanced. When adding the above ingredients, skin safety through combined use, ease of formulation, and stability of the active ingredients may be considered.

[0047] The quasi-drug composition of the present invention may further contain, as known in the industry, retinoic acid, TGF, animal placenta-derived protein, betulinic acid, and chlorella extract as anti-aging ingredients; as known in the industry, non-steroidal ingredients such as flufenamic acid, ibuprofen, benzydamine, indomethacin, prednisolone, dexamethasone, allantoin, azulene, and hydrocortisone; and one or more ingredients selected from the group consisting of derivatives thereof and various plant extracts. The additional ingredients may be included in an amount of 0.0001% to 10% by weight of the total composition weight, and the content range may be adjusted according to requirements such as skin safety and ease of formulation of the bacterial strain or ferment of the present invention.

[0048] The formulation method, dosage, method of use, and constituent components of quasi-drugs can be appropriately selected from common techniques known in the technical field.

[0049] Another aspect of the present invention provides a food composition for improving skin, comprising one or more of the following: Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, a culture of the strain, and a fermented product of the strain.

[0050] The aforementioned Saccharomyces cerevisiae, cultures, ferments, and skin improvement are as described above.

[0051] The term "food" in this invention includes all foods in the ordinary sense, such as meat, sausages, bread, chocolate, candies, snucks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinking water, tea, energy drinks, alcoholic beverages, vitamin complexes, health functional foods, and health foods.

[0052] The term "functional food" refers to a food with high medical and therapeutic effects, processed to efficiently exhibit biological regulatory functions in addition to nutrient supply. Here, "functional" means obtaining effects useful for health purposes, such as regulating nutrients or physiological effects, in relation to the structure and function of the human body. The food of the present invention can be manufactured by methods commonly used in the industry, and in its manufacture, raw materials and components that are normally added in the industry can be added. Furthermore, the dosage form of the food can be manufactured without restriction as long as it is a dosage form recognized as a food. The food composition of the present invention can be manufactured in various dosage forms, and unlike general pharmaceuticals, it has the advantage of not having side effects that can occur when pharmaceuticals are taken long-term, as it is made from food as a raw material, and is highly portable. The food of the present invention can be taken as an adjunct to enhance skin improvement effects.

[0053] The term "health food" refers to foods that have a more active effect on maintaining or promoting health compared to general foods, while "health supplement food" refers to foods intended to supplement health. In some cases, the terms "health functional food," "health food," and "health supplement food" are used interchangeably.

[0054] Specifically, the aforementioned health functional foods are foods manufactured by adding one or more of the following to food ingredients such as beverages, teas, spices, gums, and confectionery, or by encapsulating, powdering, or suspending them: Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, a culture of the strain, or a fermented product of the strain. When consumed, they are said to have specific health benefits, but unlike general medicines, they have the advantage of not having the side effects that can occur with long-term use of medicines, because they are made from food ingredients.

[0055] Since the food composition of the present invention can be consumed on a daily basis, it is expected to have a high effect on improving skin condition and is therefore extremely useful.

[0056] The composition may further contain a physiologically acceptable carrier, and the type of carrier is not particularly limited; any carrier commonly used in the art may be used.

[0057] Furthermore, the composition may contain additional components that are commonly used in food compositions to improve odor, taste, appearance, etc. Examples include vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, and pantothenic acid. It may also contain minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), and copper (Cu). It may also contain amino acids such as lysine, tryptophan, cysteine, and valine.

[0058] Furthermore, the composition may contain food additives such as preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder and high-grade bleaching powder, sodium hypochlorite, etc.), antioxidants (butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), etc.), colorants (tar dyes, etc.), color fixatives (sodium nitrite, sodium acetate, etc.), bleaching agents (sodium sulfite), seasonings (MSG, monosodium glutamate, etc.), sweeteners (dulcin, cyclamic acid, saccharin, sodium, etc.), flavorings (vanillin, lactones, etc.), leavening agents (alum, potassium bitartrate, etc.), fortifiers, emulsifiers, thickeners, coating agents, gum bases, foam inhibitors, solvents, and improvers. The additives may be selected according to the type of food and used in appropriate amounts.

[0059] One or more of the Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, the culture of the strain, and the fermented product of the strain may be added as is or used together with other foods or food ingredients, and can be used appropriately according to the usual methods. The amount of active ingredients mixed can be appropriately determined according to the purpose of use (prevention, health, or therapeutic treatment). Generally, the food composition of the present invention may be added to food or beverages in an amount of 50 parts by weight or less, specifically 20 parts by weight or less, during the manufacture of food or beverages, but is not limited thereto.

[0060] The present invention may be used as an example of a food composition in a health beverage composition, in which case it may contain various flavorings or natural carbohydrates as additional ingredients, as in ordinary beverages. The aforementioned natural carbohydrates may be monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; or sugar alcohols such as xylitol, sorbitol, and erythritol. As a sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrates is generally about 0.01 to 0.04 g per 100 mL of the composition of the present invention, specifically about 0.02 to 0.03 g.

[0061] In addition to the above, the health beverage composition may contain various nutrients, vitamins, electrolytes, flavorings, colorings, pectin, salts of pectin, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, or carbonating agents. It may also contain fruit pulp for the production of natural fruit juice, fruit juice beverages, or vegetable beverages. These components may be used individually or in combination. The proportion of such additives is not particularly important, but is generally selected from the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

[0062] The food composition of the present invention may contain various weight percentages as long as it can demonstrate a skin-improving effect. Specifically, it may contain one or more of the following in an amount from 0.00001 to 100% by weight relative to the total weight of the food composition: Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, a culture of the said strain, and a fermented product of the said strain.

[0063] Saccharomyces cerevisiae deposited under deposit number KCTC14268BP, cultures of the strain, and ferments of the strain may be included in quasi-drugs, foods, and / or cosmetic compositions as described above, but may also be included in pharmaceutical compositions.

[0064] Another embodiment of the present invention provides a method for improving skin, comprising the step of administering to an individual one or more of the following: a Saccharomyces cerevisiae strain deposited under deposit number KCTC14268BP, a culture of the strain, and a fermented product of the strain; or a food composition containing the same.

[0065] The administration is by oral administration, which may be by ingestion and / or inhalation, and the Saccharomyces cerevisiae, culture, ferment, and skin improvement are as described above.

[0066] In this invention, the term "individual" means, but is not limited to, all animals, including mammals such as mice, livestock, and humans.

[0067] Another aspect of the present invention provides a method for improving skin, comprising the step of applying to the skin of an individual one or more of the following: a Saccharomyces cerevisiae strain deposited under deposit number KCTC14268BP, a culture of the strain, and a ferment of the strain; a cosmetic composition containing the same; and / or a quasi-drug composition containing the same.

[0068] The aforementioned Saccharomyces cerevisiae, cultures, ferments, and skin improvement are as described above.

[0069] In this application, the term “application” means bringing the compositions of the present invention into contact with the skin of an individual in any suitable manner, including, but not limited to, any action aimed at causing the compositions to be absorbed into the skin.

[0070] Another aspect of the present invention provides one or more skin improvement applications of the Saccharomyces cerevisiae strain deposited under deposit number KCTC14268BP, a culture of the strain, and a ferment of the strain.

[0071] The aforementioned Saccharomyces cerevisiae, cultures, ferments, and skin improvement are as described above. [Examples]

[0072] The present invention will be described in more detail below through examples. However, these examples and experimental examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples and experimental examples.

[0073] Example 1: Isolation and selection of yeast strains from Dendrobium moniliforme

[0074] 1-1: Isolation of yeast from Dendrobium nobile

[0075] Dendrobium flowers were placed in YM broth and cultured with shaking at 30°C for 4 days. Then, they were inoculated onto solid medium and cultured at 30°C for 3 days. Colonies formed on the solid medium were isolated and identified. At this time, 187 strains presumed to be yeast (Saccharomyces genus) were isolated, and microbial identification was then carried out.

[0076] As a result, we obtained six strains classified as Saccharomyces cerevisiae and one strain classified as Saccharomyces boulardii.

[0077] The seven types of yeast (six Saccharomyces cerevisiae and one Saccharomyces boudii) secured in this invention are shown in Table 1 below.

[0078] [Table 1]

[0079] 1-2: Selection of yeast isolated from Dendrobium nobile

[0080] To select a strain from the seven aforementioned yeast fermentations that had less off-odor and was easy to use, an off-odor test was conducted on 10 subjects after the seven yeast fermentations were prepared. The indicators for the off-odor test are shown in Table 2 below.

[0081] [Table 2]

[0082] As a result, as shown in Table 3, it was confirmed that the odor associated with item 5 was the least noticeable.

[0083] [Table 3]

[0084] The Saccharomyces cerevisiae #5-31 strain of iron-skinned Dendrobium yeast secured in this invention was deposited with the Korea Institute of Biotechnology, a depositary under the Budapest Convention, on August 10, 2020, and was assigned accession number KCTC14268BP.

[0085] Example 2: Production of Dendrobium nobile yeast fermentation product

[0086] The Saccharomyces cerevisiae KCTC14268BP strain was inoculated into YPD medium and cultured with shaking at 30°C for 4 days. The cells were then separated by centrifugation. After suspending the cells in purified water, they were sonicated to disrupt the cells, and finally, a Saccharomyces cerevisiae yeast ferment was produced. The specific conditions and composition are shown in Table 4 below.

[0087] [Table 4]

[0088] Example 3: Confirmation of the collagen synthesis-enhancing effect of Dendrobium nobile yeast fermentation product.

[0089] Human dermal fibroblasts were treated with the sample for 48 hours. Collagen synthesis rates were then measured by detecting collagen using a procollagen type I C-peptide (PIP) EIA kit. The collagen synthesis efficacy of the sample was expressed as a percentage increase in collagen synthesis, calculated in comparison to a negative control group (DMEM containing 0% serum).

[0090] The formula for calculating the collagen synthesis increase rate, the samples used, and the results are shown in Table 5 below.

[0091] - Collagen synthesis increase rate (%) = (Collagen synthesis rate of the sample - Collagen synthesis rate of the negative control group) / Collagen synthesis rate of the negative control group

[0092] [Table 5]

[0093] As shown in the results in Table 5 above, it was confirmed that collagen synthesis ability increased when general cells were treated with Dendrobium nobile yeast (KCTC14268BP) ferment. At this time, the increase rate of Dendrobium nobile yeast ferment (KCTC14268BP) was superior to that of general yeast (KCTC 7296) ferment produced under the same conditions.

[0094] Therefore, it has been found that the fermented product of Dendrobium nobile yeast of the present invention exhibits excellent effects in improving skin wrinkles, increasing elasticity, and preventing aging.

[0095] Example 4: Confirmation of the hyaluronic acid synthesis-enhancing effect of Dendrobium nobile yeast fermentation product.

[0096] Each sample was treated with human keratinocyte (HaCaT) cells for 72 hours. Hyaluronic acid synthesis rates were determined by measuring hyaluronic acid using R&D Systems' Quantikin ELISA Hyaluronan. The skin moisturizing ability of the samples was expressed as the percentage increase in hyaluronic acid synthesis compared to a negative control group (treated with DMEM containing 0% serum).

[0097] The formula for calculating the hyaluronic acid synthesis increase rate, each sample, and the results are shown in Table 6 below.

[0098] - Hyaluronic acid synthesis increase rate (%) = (Hyaluronic acid synthesis rate of the sample - Hyaluronic acid synthesis rate of the negative control group) / Hyaluronic acid synthesis rate of the negative control group

[0099] [Table 6]

[0100] As shown in the results in Table 6 above, it was confirmed that hyaluronic acid synthesis ability increased when general cells were treated with a fermented product of Dendrobium nobile yeast (KCTC14268BP). At this time, the increase rate of the Dendrobium nobile yeast fermented product was superior to that of a fermented product of general yeast (KCTC 7296) produced under the same conditions.

[0101] Therefore, it was found that the fermented product of Dendrobium nobile yeast (KCTC14268BP) of the present invention exhibits excellent effects in moisturizing the skin.

[0102] From the above description, those skilled in the art in the field to which the present invention pertains will understand that the present invention can be implemented in other specific forms without altering its technical idea or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be interpreted as encompassing all modified or altered forms derived from the meaning and scope of the claims and their equivalent concepts, as described in the above detailed description.

Claims

1. A strain of Saccharomyces cerevisiae deposited under deposit number KCTC14268BP.

2. The strain according to claim 1, wherein the Saccharomyces cerevisiae is derived from Dendrobium candidum.

3. The strain according to claim 1, wherein the Saccharomyces cerevisiae is derived from the flowers of Dendrobium candidum.

4. A cosmetic composition for improving skin, comprising a fermented product of the bacterial strain described in claim 1.

5. The cosmetic composition according to claim 4, wherein the skin improvement is one or more selected from the group consisting of skin moisturizing, wrinkle improvement, elasticity enhancement, hyaluronic acid production promotion, collagen synthesis promotion, and anti-aging.

6. A quasi-drug composition for improving skin, comprising a fermented product of the bacterial strain described in claim 1.

7. The quasi-drug composition according to claim 6, wherein the skin improvement is one or more selected from the group consisting of skin moisturizing, wrinkle improvement, elasticity enhancement, hyaluronic acid production promotion, collagen synthesis promotion, and anti-aging.

8. A food composition for improving skin, comprising a fermented product of the bacterial strain described in claim 1.

9. The food composition according to claim 8, wherein the skin improvement is one or more selected from the group consisting of skin moisturizing, wrinkle improvement, elasticity enhancement, hyaluronic acid production promotion, collagen synthesis promotion, and anti-aging.

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