Elastic material and method for manufacturing the same

Abstract

To provide a novel biological functional material useful as a biomaterial.SOLUTION: The present invention relates to a gelatinous elastic material containing elastin, collagen, chitosan, and a solvent. In a preferable example, the elastin, collagen and chitosan are crosslinked with citric acid.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an elastic material using elastin, collagen, and chitosan, and a method for producing the same.

Background Art

[0002] In the industrial field of regenerative medicine for regenerating and treating damaged tissues of the human body, a biomaterial compatible with the human body is required. The performances required for the biomaterial used in regenerative medicine include being safe for the human body and having both strength and elasticity, and the development of materials having these performances is desired.

[0003] Biomaterials developed so far, for example, materials for artificial blood vessels and drug delivery system (DDS) carriers, use artificial polymers of organic compounds useful for achieving their intended uses and functions. Since these are composed of components not contained in the living body, it is difficult to use them permanently, and furthermore, there is a problem that their safety for the human body is low.

[0004] On the other hand, elastin present in elastic tissues of the living body such as blood vessels and lungs is a protein that generates the elasticity of the body, and has been expected as a material for biomaterials so far. However, due to reasons such as research being delayed because of its insolubility, it has not been able to overcome cost and technical problems, and there is almost no use as a biomaterial.

[0005] In addition, materials using biogenic polymer proteins such as collagen (gelatin) have also been studied (see, for example, Patent Documents 1 and 2). However, due to problems such as collagen having hardness (rigidity) and being inferior in elasticity, and having low strength (hard materials are brittle), it has not been widely used.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

[0007] The object of this invention is to provide a novel biofunctional material that is useful as a biomaterial. [Means for solving the problem]

[0008] As a result of diligent research to solve the above problems, the inventors of this invention discovered that an elastic material manufactured using three materials—elastin, collagen, and chitosan—is an excellent biofunctional material, and thus completed the present invention.

[0009] In other words, the present invention is as follows: [1] An elastic material characterized by containing elastin, collagen, chitosan, and a solvent. [2] The elastic material according to [1], characterized in that the solvent content is 5 to 80 w / w%. [3] The elastic material according to [1] or [2], characterized in that the elastin, collagen and chitosan are crosslinked. [4] The elastic material according to [3], characterized by being crosslinked with citric acid or a salt thereof. [5] The elastic material according to [4], characterized in that it is crosslinkable and reversible.

[0010] [6] An elastic material according to any one of [1] to [5], characterized in that the elongation rate when a tensile test is performed using a No. 6 dumbbell test specimen (JIS K6251-6) is 10% or more. [7] An elastic material according to any one of [1] to [6], characterized in that the maximum stress when a tensile test is performed using a No. 6 dumbbell test specimen (JIS K6251-6) is 0.5 MPa or more.

[0011] [8] A method for producing an elastic material, characterized by mixing elastin, collagen, and chitosan in a solvent and then solidifying the mixture. [9] The method for producing an elastic material according to [8], characterized in that, during solidification, at least one of a low-temperature drying treatment and a crosslinking treatment is performed.

[10] A method for producing an elastic material according to [9], characterized in that the low-temperature drying treatment is a drying treatment below 0°C to 15°C.

[11] A method for producing an elastic material according to any one of [8] to

[10] , characterized by adding collagen to a solvent so that the collagen concentration is 1 to 30 w / v%.

[0012]

[12] A composition for producing elastic materials, characterized by containing elastin, collagen, and chitosan.

[13] The composition for producing elastic materials according to

[12] , characterized in that the elastin, collagen, and chitosan are contained in an amount of 80% by mass or more on a solid content basis. [Effects of the Invention]

[0013] According to the present invention, it is possible to provide novel bio-functional materials that are useful as biomaterials. [Brief explanation of the drawing]

[0014] [Figure 1] This is a photograph of a citrate-crosslinked gel-like elastic material. [Figure 2] This is a photograph of a gel-like elastic material (8% elastin) immersed in a 1×PBS solution for 3 hours. [Figure 3] This is a photograph of a gel-like elastic material that has been decrosslinked by immersion in a 1×PBS solution for 3 hours. [Figure 4] This is a photograph of a gel-like elastic material that has been re-crosslinked from a decrosslinked gel-like elastic material. [Figure 5] This is a photograph of a glycine-containing gel-like elastic material. [Modes for carrying out the invention]

[0015] The elastic material of the present invention is characterized by containing elastin, collagen, chitosan, and a solvent. The elastic material of the present invention has a fine three-dimensional network structure formed based on hydrophobic interactions and hydrogen bonds between the molecules of the three components of elastin, collagen, and chitosan. In the following description, the three components of elastin, collagen, and chitosan may be referred to as the three components of the present invention.

[0016] The elastic material of the present invention has high safety for the human body and has both strength and elasticity. Therefore, it is useful as a biomaterial. For example, it can be used as an anti-adhesion material (anti-adhesion film) for preventing adhesion between biological tissues, an adhesive material for hemostasis (adhesive film for hemostasis), an artificial blood vessel, etc. It can also be used as a drug delivery system carrier for impregnating drugs and the like. It can also be used as a skin external preparation such as a wound dressing material and a face mask base material. Furthermore, it can also be used orally.

[0017] When a tensile test is performed on the elastic material of the present invention using a No. 6 dumbbell test piece (JIS K6251-6), the elongation rate is preferably 10% or more, more preferably 50% or more, further preferably 100% or more, particularly preferably 200% or more, and most preferably 300% or more. Also, when a tensile test is performed on the elastic material of the present invention using a No. 6 dumbbell test piece (JIS K6251-6), the maximum stress is preferably 0.5 MPa or more, more preferably 1.0 MPa or more, further preferably 1.5 MPa or more, and particularly preferably 2.0 MPa or more.

[0018] Elastin is a protein found in animal tissues, particularly mammalian tissues, such as the dermis, ligaments, tendons, blood vessels, and lungs. It has been reported to be a stable protein with low antigenicity. In this invention, elastin derived from such animals can be used, and commercially available products may also be used. Furthermore, water-soluble elastin is preferred in this invention. Water-soluble elastin readily interacts with gelatin molecules containing many hydrophilic amino acids, and it is believed that a more elastic three-dimensional structure can be easily constructed. As water-soluble elastin, for example, elastin obtained from animal tissue by hydrolysis with acid or alkali or enzymatic treatment is preferred. Specifically, it can be obtained, for example, by the methods described in Japanese Patent No. 4078431 and Japanese Patent No. 6712014. By including elastin together with other components, a material possessing both strength and elasticity can be obtained.

[0019] Furthermore, elastin has a cross-linking structure due to desmosines (Des+Ide). The content of desmosines in the total amino acid composition of elastin is preferably 0.1 w / w% or more, more preferably 0.5 w / w% or more, even more preferably 1.0 w / w% or more, and particularly preferably 2.0 w / w% or more.

[0020] The elastin content in the elastic material is preferably 1 to 80 w / w%, and more preferably 5 to 70 w / w%. When manufactured by low-temperature drying treatment (see Example 1), a relatively high amount of elastin is preferable, preferably 20 to 80 w / w%, and more preferably 30 to 80 w / w%. Therefore, when considering the three components of the present invention, when mixing each component in w / v%, a ratio (w / v%) where the amounts of collagen and elastin are equal or where the amount of elastin is greater than that of collagen can exhibit higher elasticity. On the other hand, when crosslinking is performed with organic acids such as citric acid (see Examples 2 and 3), a relatively low amount of elastin is preferable in order to impart sufficient elasticity, and the elastin content of the elastic material used for crosslinking is preferably 5 to 50 w / w%, and more preferably 5 to 40 w / w%. When considering the three components of this invention, when mixing the components in w / v%, it is preferable that the amounts of collagen and elastin are equal or that the amount of collagen is greater than that of elastin to achieve higher elasticity. Having elastin within this range prevents the collagen from becoming a completely rigid body consisting of its original triple helix structure from a random structure during gelation. Collagen and chitosan impart appropriate rigidity (strength) to the elastic material, and the elastin interacts with each component (primarily through hydrophobic interactions and hydrogen bonding) to form a new three-dimensional network structure different from that of collagen alone, thereby imparting appropriate elasticity to the elastic material. Furthermore, in both low-temperature drying and crosslinking with organic acids such as citric acid, it is preferable that the amount of chitosan is less than that of either collagen or elastin.

[0021] Collagen is a protein found in animals, particularly mammals and fish, in the bones, cartilage, skin, and internal organs. The collagen of this invention can be derived from such animals, and commercially available products may also be used. Furthermore, the collagen of this invention is preferably water-soluble, and the collagen of this invention includes gelatin obtained by heat-denaturing natural collagen.

[0022] The collagen (gelatin) content in the elastic material is preferably 5-80 w / w%, more preferably 10-70 w / w%, and even more preferably 10-60 w / w%. This collagen content provides the elastic material with appropriate rigidity and elasticity, while the collagen molecules strongly interact with chitosan and elastin (primarily through hydrophobic interactions and hydrogen bonding), thereby imparting appropriate rigidity (strength) to the elastic material. It is also preferable to use an elastic material with the above collagen content when crosslinking.

[0023] Chitosan is a polysaccharide having a structure in which glucosamine is linked by β1,4 bonds. In the present invention, the chitosan can be, for example, deacetylated chitin obtained from the exoskeleton of crustaceans such as shrimp and crabs, and commercially available products may also be used. The degree of deacetylation of the chitosan is not particularly limited, and for example, a deacetylation degree of 50-100%, preferably 80-100%, can be used.

[0024] The chitosan content in the elastic material is preferably 0.1 to 25 w / w%, more preferably 0.5 to 20 w / w%, and more preferably 0.5 to 15 w / w%. When the chitosan content is within this range, it strongly interacts with collagen and elastin (mainly through hydrophobic interactions and hydrogen bonding), imparting appropriate rigidity (strength) to the elastic material. Furthermore, the presence of hydrophilic groups such as hydroxyl groups and amino groups allows them to bond with water molecules, providing water retention and thus imparting appropriate moisture retention to the elastic material. Because moisture retention is ensured by chitosan, the elastic material can maintain its strength and elasticity over a long period. It is also preferable to use an elastic material with the above chitosan content when crosslinking.

[0025] The solvent (including the dispersion medium) in the elastic material of the present invention is preferably a water-soluble solvent such as water or aqueous ethanol, and water is particularly preferred. The solvent content is preferably in a range in which the material of the present invention becomes gel-like, for example, 5 to 80 w / w%, preferably 5 to 70 w / w%, more preferably 10 to 65 w / w%, even more preferably 15 to 60 w / w%, and particularly preferably 15 to 40 w / w% in the elastic material. It is also preferable to use an elastic material with the above content when used for crosslinking.

[0026] The elastic material of the present invention can be obtained by mixing a raw material composition (composition for manufacturing elastic material) containing elastin, collagen, and chitosan in a solvent, and then solidifying (gelling) it. An example of the composition for manufacturing elastic material is a mixed powder containing the three components of the present invention.

[0027] The content of the three components of the present invention, elastin, collagen, and chitosan, in the composition for manufacturing elastic materials is preferably 80 w / w% or more, more preferably 90 w / w% or more, even more preferably 95 w / w% or more, and particularly preferably 99 w / w% or more, on a solid content basis. Other components besides these three components of the present invention include, for example, stabilizers, preservatives, antioxidants, weather-resistant agents, colorants, and fragrances for maintaining and improving the performance of the elastic material itself. Furthermore, pharmaceuticals such as antibacterial substances and anti-inflammatory agents, amino acids such as glycine, vitamins, and minerals can be used to impart predetermined effects to the body when used.

[0028] As described above, solidification is necessary to produce an elastic material using the composition for producing elastic materials. The amount of collagen added to the solvent is preferably such that the collagen concentration is 1-30 w / v%, more preferably 10-30 w / v%, and more preferably 15-30 w / v%. Using a high-concentration collagen solution allows for the production of a superior gel-like elastic material. In the following description, the gel-like elastic material may also be referred to as a gel-like elastic material or gel-like material.

[0029] The method for solidifying (gelling) the three components of the present invention is not particularly limited and can include low-temperature drying or crosslinking. This makes it possible to obtain a material with superior strength and elasticity.

[0030] For the low-temperature drying treatment, it is preferable to dry slowly at a low temperature of, for example, greater than 0°C to 15°C, preferably 1 to 10°C. The low-temperature drying treatment is carried out so that the solvent content in the elastic material is, for example, 5 to 80 w / w%, preferably 5 to 70 w / w%, more preferably 10 to 65 w / w%, even more preferably 15 to 60 w / w%, and particularly preferably 15 to 40 w / w%.

[0031] Furthermore, various methods using crosslinking agents can be used for the crosslinking treatment. The crosslinking treatment can be performed after mixing the three components of the present invention, or it may be performed after solidification (gelation) by the low-temperature drying treatment described above. In the latter case, for example, the solvent content of the gel-like substance may be greater than 40-90 w / w% or greater than 60-90 w / w%.

[0032] As a crosslinking agent, one that is highly safe for living organisms is preferred. Examples include organic acids such as citric acid, ferulic acid, tannic acid, acetic acid, succinic acid, lactic acid, tartaric acid, glycolic acid, ascorbic acid, fumaric acid, phytic acid, caffeoylquinic acid, or salts thereof. Among these, citric acid is particularly preferred because it exhibits high crosslinkability.

[0033] As described above, the elastic material crosslinked using citric acid in the present invention is reversible. That is, the citric acid used as a crosslinking agent can be removed from the crosslinked elastic material, and its strength and elasticity can be restored by crosslinking it again with citric acid.

[0034] Reversible crosslinked elastic materials are considered to be highly biocompatible materials with rapid biodegradability and high safety in the body. Furthermore, these properties mean that even if damaged by vigorous movement, a protective film can be formed on the damaged area with a small amount of moisture and heating. In addition, citrate-crosslinked elastic materials are expected to remain in the body for a longer period of time while maintaining their specified strength and elasticity compared to uncrosslinked elastic materials. Conventional crosslinked elastic materials are prone to damage, perforation, and displacement from the protected area when implanted in the body due to strenuous exercise. In such cases, film replacement through re-operation becomes necessary. In contrast, the decrosslinkable elastic material of the present invention absorbs moisture from the body in response to strenuous movement of the affected area, decrosslinking and slowly dissolving. As a result, the dissolved components, when exposed to a small amount of moisture and heat, can form a film on the damaged area, which is expected to improve the repair of the damaged area and the adhesion of the protected area.

[0035] The shape of the elastic material of the present invention is not particularly limited and can be in the form of a sheet, thread, rod, pellet, tube, etc. [Examples]

[0036] The present invention will be described in detail below with reference to examples, but the technical scope of the present invention is not limited to these examples. In these examples, tests were conducted using gelatin having properties equivalent to those of collagen. Hereafter, unless otherwise specified, "%" represents "w / v%".

[0037] [Example 1] <Preparation of gel-like materials by slow drying at low temperatures> Elastin (8%, 16%, 25%), gelatin (5%, 10%, 20%), and chitosan (1%) were mixed with water and then slowly dried at 4°C until the moisture content reached 20% of the initial moisture content to obtain the gel-like elastic material of Example 1. Specifically, the gel weight that would result in the desired residual moisture content (initial moisture ratio (20%) when the initial moisture content is 100%) was calculated from the measured weight a of the gel-like elastic material immediately after solidification, and the material was dried until it reached that weight. The elastin used in this example was derived from the aorta of a pig, and the content of desmosines (Des+Ide) in the total amino acid composition of the elastin was approximately 2.5 w / w% (the same applies below).

[0038] The proportions of each component in the manufactured gel-like elastic composition are shown in Table 1 below. The proportion of each component in the manufactured gel-like elastic composition was calculated based on the actual weight a of the gel-like elastic material immediately after solidification (the actual weight of the gel-like elastic material before drying). Note that "initial %" refers to the ratio (w / v%) when each component is mixed. Elastin content = Actual weight a × Initial elastin percentage Gelatin content = Actual weight a × Initial gelatin percentage Chitosan amount = actual weight a × initial chitosan percentage Moisture content = Actual weight a - Elastin content - Gelatin content - Chitosan content

[0039] [Table 1]

[0040] The obtained gel-like elastic material was punched out using a No. 6 dumbbell test specimen (JIS K6251-6), and tensile tests were performed using a precision universal tester (Autograph EZ-LX 1kN (manufactured by Shimadzu Corporation)). The elongation rate was calculated from the amount of displacement between the gauge marks. The tensile tests were commissioned to the Chemicals Evaluation and Research Institute (CERI) (the same applies to Examples 2 and 3).

[0041] Table 2 shows the tensile test results of the gel-like elastic material from Example 1, which was slow-dried at low temperature. In the table, DE is an abbreviation for slow-dried at low temperature, and CA, described later, is an abbreviation for citric acid crosslinking treatment.

[0042] [Table 2]

[0043] As shown in Table 2, the various gel-like elastic materials of Example 1, prepared with the component ratios shown in Table 1, showed an increasing trend in maximum stress and elongation rate as the elastin content increased from 8% to 25%. In other words, it was shown that the gel-like elastic materials of Example 1, produced by low-temperature slow drying, gained strength and elasticity (elongation) in an elastin concentration-dependent manner.

[0044] [Example 2] <Preparation of gel-like materials by citrate crosslinking> Elastin (8%, 16%, 25%), gelatin (20%), and chitosan (1%, 5%) were mixed with water, and then cooled and solidified at 4°C for 24 hours to obtain a gel-like material (initially with a water content of approximately 100%). This gel-like elastic material was then immersed in a 1.2 M citric acid solution (pH 7.2) at room temperature for 72 hours to obtain the gel-like elastic material of Example 2. A tensile test was performed on the obtained gel-like elastic material in the same manner as in Example 1.

[0045] The proportions of each component in the gel-like elastic composition used for crosslinking are shown in Table 3 below.

[0046] [Table 3]

[0047] Table 4 shows the tensile test results of the gel-like elastic material of Example 2, which was cross-linked with citrate.

[0048] [Table 4]

[0049] As shown in Table 4, the various gel-like elastic materials of Example 2, prepared with the component ratios shown in Table 3, exhibited significantly improved maximum stress and elongation compared to the low-temperature, slow-drying gel-like elastic material of Example 1. The above demonstrates that crosslinking the components contained in the gel-like material with citric acid adds strength and elasticity (stretchability).

[0050] [Example 3] <Preparation of gel-like materials by low-temperature slow drying and citrate crosslinking> Similar to Example 1, gel-like materials (8%, 16%, and 25% elastin) were slowly dried at low temperature to a moisture content of 20% of their initial moisture content. These were then crosslinked with citric acid, as in Example 2, to obtain the gel-like elastic material of Example 3. A tensile test was performed using the obtained gel-like elastic material, as in Example 1.

[0051] The proportions of each component in the gel-like elastic composition used for crosslinking are shown in Table 5 below.

[0052] [Table 5]

[0053] Table 6 shows the tensile test results of the gel-like elastic material from Example 3, which was cross-linked with citrate after slow drying at low temperature.

[0054] [Table 6]

[0055] As shown in Table 6, the various gel-like elastic materials of Example 3, prepared with the component ratios shown in Table 5, exhibited significantly improved maximum stress and elongation compared to the low-temperature, slow-drying gel-like elastic material of Example 1. The above findings demonstrate that even in dried gel-like materials, crosslinking the components contained in the gel-like material with citric acid adds strength and elasticity (extension). Furthermore, it was found that when crosslinking with citric acid, a tendency for higher maximum stress and elongation rates is observed when the amount of elastin is relatively small.

[0056] Furthermore, the following table compares the elastic modulus in the manufacturing methods of Examples 1 to 3, specifically when no elastin is added and when 8% elastin is added (initial). As shown in Tables 7 to 9, it was demonstrated that the elongation rate is greatly improved by adding elastin.

[0057] [Table 7]

[0058] [Table 8]

[0059] [Table 9]

[0060] [Example 4] <Reversible Bridge Cross-linking Test> Elastin (8%, 16%), gelatin (20%), and chitosan (1%) were mixed with water, and then cooled and solidified at 4°C for 24 hours (initial water content approximately 100%). The resulting gel-like material was then immersed in 0.6M, 0.8M, and 1.2M citric acid solutions at room temperature for 72 hours to crosslink the material. For comparison, an uncrosslinked gel-like material was also prepared. Photographs of the crosslinked gel-like elastic material are shown in Figure 1. In Figure 1, (1) represents the uncrosslinked material, (2) represents the 0.6M citric acid crosslinked material, (3) represents the 0.8M citric acid crosslinked material, and (4) represents the 1.2M citric acid crosslinked material.

[0061] Next, each cross-linked gel material was decrosslinked in a 1×PBS solution (phosphate-buffered saline; 0.2 g / L potassium dihydrogen phosphate, 0.2 g / L potassium chloride, 1.15 g / L disodium hydrogen phosphate, 8 g / L sodium chloride) at 37°C for 3 hours, and the state of each gel material was observed.

[0062] Figure 2 shows a photograph of an elastin-containing gel-like elastic material immersed in a 1×PBS solution for 3 hours. As shown in Figure 2, the uncrosslinked gel completely disintegrated to the point where its shape could not be confirmed, and the solvent became cloudy. Some gel disintegration and solvent cloudiness were also observed in the 0.6M citrate-crosslinked gel. On the other hand, the 0.8M and 1.2M citrate-crosslinked gels mostly retained their shape and remained intact. Therefore, citrate-crosslinked gels are expected to remain in vivo for a longer period than uncrosslinked gels.

[0063] After another three hours, the remaining gel-like material was carefully removed from the PBS solution and observed in detail.

[0064] Figure 3 shows the characteristics of each gel-like material. As shown in Figure 3, each citrate-crosslinked gel remained in the solution, maintaining its shape even after 3 hours. The maximum stress and elongation were too weak to measure, with a maximum stress of 0 MPa and an elongation of 0%, indicating that decrosslinking had occurred.

[0065] Subsequently, the decrosslinked gel-like materials were immersed in 0.8 and 1.2 M citric acid solutions, respectively, at room temperature for 72 hours to recrosslink them. Figure 4 shows a photograph of the recrosslinked gel-like elastic material.

[0066] Furthermore, after the reaction was complete, tensile tests were performed on each of the re-crosslinked gel-like materials (8% elastin (RE-8), 16% elastin (RE-16)).

[0067] Table 10 shows the tensile test results for gel-like elastic materials recrosslinked with 0.8 M citric acid solution, and Table 11 shows the tensile test results for gel-like elastic materials recrosslinked with 1.2 M citric acid solution. These tensile tests were performed using a No. 8 dumbbell test specimen (JIS K6251-8) on an "MCT-2150 tensile testing machine" installed in the laboratory, and the elongation rate was calculated using the accompanying software MSAT-Lite. For comparison, gel-like materials (E8CA) obtained by crosslinking 8% elastin-containing gel-like materials with 0.8 and 1.2 M citric acid solutions were used.

[0068] [Table 10]

[0069] [Table 11]

[0070] A comparison of RE-8 and E8CA with 8% elastin content revealed that the maximum stress recovered to slightly over 40% in the 0.8M citrate-recrosslinked gel, and to approximately 70% in the 1.2M citrate-recrosslinked gel. Furthermore, the elongation rate recovered to approximately 70-80%.

[0071] From the above, it was revealed that the citrate-crosslinked gel-like elastic material can be recrosslinked, and its maximum stress and elongation rate can be recovered to about 70% by a 1.2M citrate solution.

[0072] [Example 5] <Preparation of glycine-containing gel-like elastic material> Elastin (8%, 16%), gelatin (20%), and chitosan (1%) were mixed with 10 g or 20 g / 100 ml of glycine aqueous solution and cooled and solidified at 4°C for 24 hours (initial water content approximately 100%). Next, gel-like elastic materials were prepared by slow drying at 4°C for 72 hours or by crosslinking with a 1.2 M citric acid solution.

[0073] Figure 5 shows photographs of the prepared glycine-containing gel-like elastic material. The left image shows the material prepared using a 10g / 100ml glycine aqueous solution, and the right image shows the material prepared using a 20g / 100ml glycine aqueous solution. (1) shows the uncrosslinked material, (2) shows the material prepared using low-temperature slow drying, and (3) shows the material prepared using citric acid crosslinking.

[0074] As shown in Figure 5, white crystals were observed in the glycine-containing gel-like elastic material. These are thought to be due to the precipitation of glycine due to the evaporation of water, suggesting that the gel-like elastic material of the present invention can embed reagents such as glycine depending on the amount of water.

[0075] Subsequently, manual tensile tests were performed using the prepared glycine-containing gel-like elastic material.

[0076] Uncrosslinked glycine-containing gel-like elastic materials (10 g and 20 g / ml glycine) exhibited a slightly more extensible feel compared to gel-like elastic materials that did not contain glycine. [Industrial applicability]

[0077] The elastic material manufacturing composition of the present invention is industrially useful because it is expected to be used as a variety of medical materials.

Claims

1. An elastic material containing water-soluble elastin, gelatin, chitosan, and a solvent, The water-soluble elastin content is 1 to 80 w / w%, The gelatin content is 5 to 80 w / w%, The chitosan content is 0.1 to 25 w / w%, The solvent content is 5 to 80 w / w%. An elastic material characterized by the following features.

2. The elastic material according to claim 1, characterized in that the water-soluble elastin, gelatin, and chitosan are crosslinked.

3. The elastic material according to claim 2, characterized by being crosslinked using citric acid or a salt thereof.

4. The elastic material according to claim 3, characterized by having crosslinking reversibility.

5. An elastic material according to any one of claims 1 to 4, characterized in that the elongation rate when a tensile test is performed using a No. 6 dumbbell test specimen (JIS K6251-6) is 10% or more.

6. An elastic material according to any one of claims 1 to 5, characterized in that the maximum stress when a tensile test is performed using a No. 6 dumbbell test specimen (JIS K6251-6) is 0.5 MPa or more.

7. A method for producing the elastic material described in Claim 1, A method for producing an elastic material, characterized by mixing water-soluble elastin, gelatin, and chitosan in a solvent and then solidifying the mixture.

8. The method for producing an elastic material according to claim 7, characterized in that, during the solidification process, at least one of the following treatments is performed: a low-temperature drying treatment and a crosslinking treatment.

9. The method for producing an elastic material according to claim 8, characterized in that the low-temperature drying treatment is a drying treatment at a temperature of 0°C to 15°C.

10. A method for producing an elastic material according to any one of claims 7 to 9, characterized in that gelatin is added to the solvent so that the gelatin concentration is 1 to 30 w / v%.

Images