Compositions and methods for treating Netherton syndrome using LEKTI-expressing recombinant microorganisms

Abstract

The present disclosure provides, inter alia, engineered microorganisms expressing recombinant LEKTI domains that are effective in treating or ameliorating symptoms of Netherton syndrome. In certain embodiments, compositions, methods, and kits comprising the microorganisms expressing the LEKTI domains are provided. [Selection diagram] None

Description

[Technical Field]

[0001] Related applications

[0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 521,050, filed on 16 June 2017, the entirety of which is incorporated herein by reference.

[0003] Technical field This disclosure relates to methods, kits, and compositions for treating Netherton syndrome or improving its effects using one or more recombinant microorganisms genetically modified to express one or more therapeutic LEKTI domains on the skin of a subject. [Background technology]

[0004] The epidermis, or stratified squamous epithelium of the skin, consists of multiple sublayers and is one of the body's most important barriers to the outside world. The stratum corneum is the outermost layer of the epidermis and develops as a result of the final anucleated step in keratinocyte differentiation from cells in the nucleated epidermal layer. While the stratum corneum is recognized as the most important physical barrier, the nucleated epidermal layer is also important in barrier function (Proksch, Brandner et al., 2008). At the same time, the skin barrier protects against widespread water loss in one direction (internal-external barrier) and against the intrusion of harmful substances from the environment (external-internal barrier) (Proksch, Brandner et al., 2008). Maintaining the barrier is also important for balanced proliferation of the basal layer and the maintenance of the calcium ion gradient, and thus for proper epidermal differentiation (Lee, Jeong et al., 2006).

[0005] There are currently several limitations to skin treatments. Many treatments, such as topical corticosteroids or biopharmaceuticals, do not address the fundamental problems of endogenous protein deficiencies in the epidermis or imbalances in microbial diversity in the skin. Recombinant proteins are a promising group of therapeutic agents in the treatment of skin diseases, but their use in relation to skin presents several challenges.

[0006] Traditional methods involve purifying and concentrating recombinant proteins extracted from bacterial systems, and then incorporating such preparations into delivery systems. Recombinant protein purification is often a very expensive method for obtaining the protein. Furthermore, several problems are associated with these traditional methods, including proteolytic degradation, inefficient delivery, and the need for long-term, repeated application to achieve therapeutic effects.

[0007] One skin condition that would benefit from improved treatment modalities is Netherton syndrome (NS). NS is a rare autosomal cutaneous disorder that manifests as severe skin inflammation and scaling, hair shaft defects, persistent allergic symptoms, and immune system problems. Newborns with NS often have red, scaly skin that can leak fluid, leading to a risk of dehydration and infection of the skin or system. Affected children are also unable to grow at a normal rate. While the health of older children and adults with NS typically improves, these individuals are often underweight and short. Many people with NS also have immune system problems such as food allergies, hay fever, asthma, or eczema.

[0008] NS is caused by a loss-of-function defect in the SPINK5 gene (a Cazal-type 5 serine protease inhibitor) that encodes the lymphoepithelial Cazal-type 5 associated inhibitor type 5 (LEKTI) protein. LEKTI is a multi-domain serine protease inhibitor normally expressed in all stratified epithelial cells and Hassall bodies of the thymus. Among the cluster of other SPINK genes (e.g., SPINK6 and SPINKI9), the SPINK5 gene encoding LEKTI is located on chromosome 5 and contains 33 exons encoding 15 inhibitory domains separated by a linker region. SPINK5 stands out among the other SPINK genes for the large number of inhibitory domains it encodes. Furthermore, the SPINK5 gene is transcribed into three different transcripts, resulting in three different LEKTI proteins with different C-terminal regions: a 145 kDa full-length protein with inhibitory domains D1-D15, a 125 kDa (short) protein with inhibitory domains D1-D12, and a 148 kDa (long) protein with an elongation linker region 13.

[0009] The LEKTI protein is a Khazar-type associated inhibitor. The Khazar motif is defined by the presence of six cysteine ​​residues located at specific distances that allow for the formation of three disulfide bonds in 1-5, 2-4, and 3-6 patterns. Two domains of LEKTI (D2 and D5) form this six-cysteine ​​motif, while the other domain shares four cysteine ​​residues, forming a rigid inhibitory loop that is thought to mimic the substrate of the target protease and inactivate the target protease catalytic site.

[0010] LEKTI proteins require proteolytic cleavage to activate their inhibitory function against many proteases. Specifically, the full-length protein is cleaved into domains D1–D5 and D6–D15. The D6–D15 domain is then further cleaved in multiple steps into D6–D9 and D10–D15, →D6 and D7–D9→D7 and D8–D9→D8. This process results in LEKTI proteins containing one and six inhibitory domains, each protein having a different inhibitory function. For example, various LEKTI inhibitory fragments can inhibit various kallikrein-related peptidases (KLKs), such as KLK5, KLK7, and KLK14.

[0011] Deficited LEKTI proteins can result from substitution, insertion, or deletion mutations in the SPINK5 gene, often causing nonsense or frameshift mutations that result in premature stop codons. Other mutations in the splice site bases can lead to abnormal splicing events in the transcribed SPINK5 gene. Thus, many SPINK5 mutations result in the complete absence of LEKTI domain synthesis. LEKTI deficiency or LEKTI loss can lead to disorientation of protease activity, causing skin desquamation and epidermal permeability due to impaired epidermal differentiation and lipid metabolism, resulting in a compromised skin barrier. Furthermore, disorientation of the activity of some KLK proteins can lead to desmosome cleavage and stratum corneum separation.

[0012] Netherton syndrome is a rare disease for which no specific treatment is available. Given the above, there is a need for novel therapeutic agents for the treatment of NS. This application aims to address these and other needs. [Prior art documents] [Non-patent literature]

[0013] [Non-Patent Document 1] Proksch, E., JM Brandner and JM Jensen (2008). "The skin: an indispensable barrier." Exp Dermatol 17(12): 1063-1072 [Overview of the project]

[0014] In one embodiment, the present disclosure provides a composition for the treatment of a skin disease, comprising a microorganism genetically modified to express and result in one or more LEKTI protein domains on mammalian skin, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the serine protease activity of at least one serine protease in or on mammalian skin.

[0015] According to some embodiments, microorganisms are adapted to survive on the surface of mammalian skin for a controlled period to provide a continuous supply of LEKTI protein domains. According to some embodiments, LEKTI protein domains are effective in improving the symptoms of Netherton syndrome. In one embodiment, the LEKTI domain is domain 6.

[0016] According to some embodiments, microorganisms are genetically modified by transfection / transformation using recombinant DNA plasmids encoding LEKTI protein domains. In some embodiments, the LEKTI domain is functionally ligated to one or more recombinant protein domains that are effective in enhancing secretion from the microorganism and / or permeability through mammalian skin. According to some embodiments, at least one LEKTI domain is functionally ligated to a SecA domain. According to some embodiments, at least one LEKTI domain is functionally ligated to an RMR domain.

[0017] According to some embodiments, at least one LEKTI domain comprises the amino acid sequence set forth in SEQ ID NO: 1.

[0018] According to some embodiments, the microorganism is adapted to grow (reproduce) on the skin of a mammal.

[0019] According to some embodiments, the expression of at least one LEKTI domain is controlled by an operon, and the amount of LEKTI provided to the skin of a mammal is proportional to the availability of external factors. In some embodiments, the expression of at least one LEKTI domain is controlled by a promoter that is constitutively active.

[0020] According to some embodiments, the microorganism is genetically modified by transfection / transformation with a recombinant DNA plasmid encoding a LEKTI protein domain and one or more antibiotic resistance genes.

[0021] According to some embodiments, the microorganism is selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[0022] According to one aspect, the present disclosure provides a method for treating or ameliorating the effects of a skin disease in a mammal that requires it, comprising providing a microorganism genetically modified to express one or more LEKTI protein domains on the surface of the skin of the mammal, wherein the LEKTI protein domain is effective to penetrate one or more layers of the skin of the mammal and is effective to inhibit the activity of at least one serine protease in or on the skin of the mammal.

[0023] According to some embodiments, the microorganism is adapted to survive for a controlled period of time on the surface of the skin of the mammal to provide a continuous supply of the LEKTI protein domain.

[0024] According to another aspect, the present disclosure provides a kit for treating or ameliorating the effects of a skin disease in a mammal that requires it, comprising (1) a composition comprising a microorganism genetically modified to express one or more LEKTI protein domains, wherein the LEKTI protein domain is effective to penetrate one or more layers of the skin of the mammal and is effective to inhibit the serine protease activity of at least one serine protease in or on the skin of the mammal, and (2) a reagent for applying the composition to the skin of the mammal.

[0025] According to some embodiments, the microorganism is adapted to survive for a controlled period of time on the surface of the skin of the mammal to provide a continuous supply of the LEKTI protein domain.

[0026] According to one aspect, the present disclosure provides a composition for treating skin diseases comprising a microorganism containing the pJB38-LEKTI complete plasmid construct.

[0027] According to some embodiments, the microorganisms are selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[0028] According to one embodiment, the present disclosure provides a composition comprising a pJB38-LEKTI complete plasmid construct. According to several embodiments, the pJB38-LEKTI complete plasmid construct is expressed in microorganisms selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, or mixtures thereof. [Brief explanation of the drawing]

[0029] [Figure 1] Figure 1 shows a vector construct containing the therapeutic LEKTI domain of the present invention. The protein-coding regions of the plasmid are ligated together in a functional manner and contain SecA, 6xHis tags, LEKTI D8-11, and RMR tags, under the control of the CmR promoter. [Figure 2] Figure 2 shows vector constructs of the pJB38 plasmid according to several embodiments of the present invention. [Figure 3] Figure 3 shows the domains of the full-length LEKTI polypeptide. [Figure 4] Figure 4 shows the SDS-PAGE results indicating that LEKTId6 is highly soluble in E. coli BL21(De3). [Figure 5] Figure 5 shows the SDS-PAGE results demonstrating the success of affinity purification of H6-LEKTId6 (8.8 kDa). [Figure 6] Figure 6 shows the SDS-PAGE results indicating that LEKTId6-H6 (8.8kDa) is potentially N-terminally truncated. [Figure 7] Figures 7A and 7B show that recombinantly produced LEKTI domain 6 inhibits trypsin in vitro. Figure 7A is a schematic diagram of the experiment performed. Figure 7B is a graph showing trypsin activity. [Figure 8] Figures 8A and 8B show that recombinant LEKTI domain 6 (ct His6 tag) inhibits trypsin in vitro compared to LEKTI domains 10-15. Figure 8A is a schematic diagram of the experiment performed. Figure 8B is a graph showing trypsin activity. [Figure 9] Figures 9A and 9B show that recombinant LEKTI domain 6 inhibits KLK7 in vitro, similar to the inhibition of KLK7 by LEKTI domains 10-15. Figure 9A is a schematic diagram of the experiment performed. Figure 9B is a graph showing KLK7 activity. [Figure 10] Figures 10A and 10B show that recombinantly produced LEKTI domain 6 inhibits KLK5 in vitro at nanomolar concentrations. Figure 10A is a schematic diagram of the experiment performed. Figure 10B is a graph showing KLK5 activity. [Modes for carrying out the invention]

[0030] One aspect of this disclosure provides a skin-resident bacterium that has been genetically modified to express a recombinant protein for treating or improving Netherton syndrome. The genetically modified protein-producing bacterium can treat NS by expressing and, if necessary, secreting a therapeutic protein that addresses the underlying cause of the disease or its symptoms. According to some embodiments, the therapeutic protein comprises one or more LEKTI domains that are effective in inhibiting serine proteases in or on mammalian skin. According to some embodiments, the recombinant LEKTI domain compensates for a deficiency in the endogenous LEKTI protein naturally produced by the skin in mammals. According to some embodiments, the genetically modified bacterium can self-replicate while retaining the ability to produce recombinant proteins, thereby resulting in a continuous supply of the therapeutic agent.

[0031] According to some embodiments, the present disclosure provides compositions for the treatment of skin diseases, comprising a microorganism genetically modified to express and result in one or more LEKTI protein domains on mammalian skin, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the serine protease activity of at least one serine protease in or on mammalian skin.

[0032] As used herein, the term “skin disease” and its grammatical variations mean a skin condition or state of human skin that is generally undesirable or harmful compared to a normal or baseline state. Examples of abnormal skin conditions include, but are not limited to, Netherton syndrome, psoriasis, acne, atopic dermatitis, allergic contact dermatitis, exfoliative hyperkeratosis, seborrheic dermatitis, eczema, dry skin, allergies, rashes, UV-irritated skin, detergent-irritated skin (including irritation caused by molecules used in enzymes and detergents and sodium lauryl sulfate), skin thinning (e.g., skin of the elderly and children), bullous pemphigoid, pemphigus vulgaris, impetigo, vitiligo, alopecia, and hirsutism.

[0033] As used herein, the terms “genetically modified” and their grammatical variations are used to describe microorganisms (e.g., bacteria) that have been genetically modified or manipulated by the introduction of DNA prepared outside the microorganism. For example, by introducing plasmid DNA containing new genes into bacteria, the bacteria can express these genes. Alternatively, DNA containing new genes can be introduced into bacteria and then incorporated into the bacterial genome, where the bacteria will express these genes.

[0034] As used herein, the terms “to treat,” “to treat,” “treatment,” and their grammatical variations mean providing a subject, e.g., a patient, with a protocol, regimen, process, or treatment that is desirable for obtaining a physiological response or outcome in that subject. In particular, the methods and compositions of the present invention can be used to slow the development of disease symptoms, delay the onset of a disease or condition, or halt the progression of disease development. However, since not all treated subjects may respond to a particular treatment protocol, regimen, process, or treatment, a treatment does not require that the desired physiological response or outcome be achieved in all subjects or subject groups, e.g., patient populations. Thus, a given subject or subject group, e.g., patient populations, may not respond to a treatment, or may respond inadequately.

[0035] In the present invention, the subject may be a mammal. As used herein, “mammal” and its grammatical variations mean any category of mammal. In the present invention, examples of mammals include humans, agricultural animals, livestock, and laboratory animals. Some examples of agricultural animals include cattle, pigs, horses, and goats. Some examples of livestock include dogs and cats. Some examples of laboratory animals include primates, rats, mice, rabbits, and guinea pigs. Preferably, the mammal is a human.

[0036] Where used herein, the terms “effective amount” or “therapeutably effective amount” of a compound or composition disclosed herein means the amount of such compound or composition that, when administered to a subject, is sufficient to produce the beneficial or desirable results described herein. Effective dosage forms, modes of administration, and doses can be determined empirically, and such determinations are within the scope of the art of those skilled in the art. Those skilled in the art will understand that doses vary depending on the route of administration, elimination rate, duration of treatment, identity of any other drugs administered, the mammal, e.g., age, size, and species of the human patient, and similar factors well known in the fields of medicine and veterinary medicine. Generally, a preferred dose of a composition according to the present invention would be the amount of the composition that is the minimum dose effective to produce the desired effect. An effective dose of a composition of the present invention may be administered as two, three, four, five, six or more subdoses, administered separately at appropriate intervals throughout the day.

[0037] Microbial Compositions: According to several embodiments, the present disclosure provides microbial compositions comprising one or more diverse bacteria suitable for use on mammalian skin. Examples, but not limited to, include non-pathogenic bacteria and commensal bacteria. Suitable bacteria for use in the present invention include, but not limited to, Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus (e.g., S. epidermidis and / or S. hominis), Lactobacillus (e.g., L. acidophilus), Pediococcus, Leuconostoc, or Oenococcus. According to some embodiments, the microbial composition includes one or more of the following: Staphylococcus warneri, Streptococcus pyogenes, Streptococcus mitis, Propionibacterium acnes, Corynebacterium spp., Acinetobacter johnsonii, and Pseudomonas aeruginosa. According to some embodiments, other relevant or similar species found on the skin are used.

[0038] Certain embodiments involve the use of Staphylococcus epidermidis bacteria. According to some embodiments, the strains of S. epidermidis used are unable to produce biofilms. Examples of these include S. epidermidis strains ATCC 12228 or NRRL B-4268.

[0039] According to some embodiments, recombinant microorganisms are adapted to survive on the surface of mammalian skin for an unlimited or controlled period to provide a continuous supply of the LEKTI protein domain. In some embodiments, recombinant microorganisms survive alongside naturally occurring commensal microorganisms on mammalian skin. In some embodiments, recombinant microorganisms survive excluding naturally occurring commensal microorganisms on mammalian skin. According to some embodiments, recombinant microorganisms are adapted to grow on mammalian skin. In other embodiments, recombinant microorganisms are no longer viable but contain an effective amount of therapeutic polypeptide, e.g., LEKTI or its therapeutically effective domain. Such cells may or may not be damaged, depending on the details of delivering the therapeutic peptide (or its domain) to the target site.

[0040] As used herein, the term “recombinant” and its grammatical variations mean relating to or referring to an organism, protein, or genetic material formed by or using recombinant DNA, which contains DNA fragments derived from different sources or from different parts of the same source. For example, the term “recombinant DNA” means a DNA molecule formed by a recombination method for splicing DNA fragments derived from different sources or from different parts of the same source. In some embodiments, two or more different sources of DNA are cut using restriction enzymes and joined together using ligases. As another example, the term “recombinant protein” or “recombinant domain” and its grammatical variations mean a protein molecule formed by a recombination method originating from spliced ​​DNA fragments derived from different sources or from different parts of the same source. As yet another example, the term “recombinant microorganism” or “recombinant bacterium” and its grammatical variations mean a microorganism / bacterium containing one or more recombinant DNA / protein molecules.

[0041] According to some embodiments, the microorganisms are selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus (e.g., S. epidermidis and / or S. hominis), Lactobacillus (e.g., L. acidophilus), Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[0042] LEKTI gene: According to some embodiments, recombinant microorganisms are engineered to express a mammalian gene encoding the LEKTI protein. The LEKTI gene can be obtained from any mammal, such as a mouse, rat, rabbit, goat, sheep, horse, cattle, dog, primate, or human gene sequence. According to some embodiments, the LEKTI gene sequence is a human gene sequence. According to some embodiments, recombinant microorganisms are engineered to contain a fragment of the LEKTI gene.

[0043] According to some embodiments, the recombinant protein expressed by the engineered microorganism includes the peptide sequence (LEKTI D8-D11) described in SEQ ID NO: 1. According to some embodiments, the recombinant protein expressed by the engineered microorganism includes the peptide sequence described in SEQ ID NO: 2. According to some embodiments, one or more fragments of the peptide sequence described in SEQ ID NO: 2 are expressed by the engineered microorganism. In one embodiment, the fragment includes one or more LEKTI domains. In one embodiment, the LEKTI domain is domain 6.

[0044] According to some embodiments, recombinant microorganisms include sequences disclosed herein that have at least about 75% identity, or 80% identity, or 85% identity, or 90% identity, or 95% identity to one or more of the sequence numbers listed herein. The term “identity” as used herein and its grammatical variations mean the degree to which two nucleotide or amino acid sequences have the same residues at the same positions in an alignment. The percentage of identity (%) is calculated by multiplying the number of matches in the sequence alignment by 100 and dividing by the length of the aligned region, including internal gaps.

[0045] According to some embodiments, the recombinant protein expressed by the engineered microorganism contains one or more protease inhibitory domains of the LEKTI protein. Some non-limiting examples include one or more of domains D1, D2, D3, D4, D5, D6, D7, D8, D9, D10, D11, D12, D13, D14, and D15. According to some embodiments, the recombinant protein expressed by the engineered microorganism contains LEKTI inhibitory domain 6 or domains D8-D11.

[0046] According to several embodiments, the LEKTI protein domain is effective in improving the symptoms of Netherton syndrome. As used herein, the terms “improve,” “to improve,” and their grammatical variations mean reducing the severity of the symptoms of the disease in the subject. In some embodiments, the LEKTI protein domain acts as a competitive or non-competitive inhibitor of one or more proteases present in or on the skin of mammals. In some embodiments, the LEKTI protein domain acts as a serine protease inhibitor. As used herein, the terms “protease” and “proteinase” are used interchangeably, and both terms refer to enzymes that perform protein lysis.

[0047] According to some embodiments, microorganisms are genetically modified by transfection / transformation using recombinant DNA plasmids encoding the LEKTI protein domain. Other conventional or discoverable methods for introducing DNA into microorganisms may also be used in the present invention. According to some embodiments, the recombinant DNA plasmid comprises a sequence encoding the LEKTI protein domain and one or more secretory peptides and / or cell permeability peptides. According to some embodiments, the LEKTI domain is functionally ligated to one or more recombinant protein domains that are effective in enhancing secretion from microorganisms and / or permeability through mammalian skin.

[0048] The term "functionally linked" refers to the linking of nucleic acid sequences on a single nucleic acid fragment in such a way that the function of one is neither regulated nor inhibited by the other. For example, a promoter is functionally linked to a coding sequence if it can regulate the expression of that coding sequence (i.e., the coding sequence is under the transcriptional control of the promoter). A coding sequence can be functionally linked to a regulatory sequence in either sense or antisense orientation. In another example, two proteins can be functionally linked so that the function of either protein is not impaired. In general, functionally linked means that the nucleic acid sequences to be linked are contiguous, and if two protein coding regions need to be linked, they must be contiguous and in the same reading frame.

[0049] As used herein, the terms “secretory peptide,” “secretory sequence,” “secretory tag,” “signal peptide,” or “transition signal,” and their grammatical variations, mean any peptide sequence capable of targeting a synthesized protein to a cellular secretory pathway. In some embodiments, the secretory peptide can be positioned on the N-terminus of a recombinant protein to target a tagged protein for secretion, either concurrently with or after translation. According to some embodiments, at least one LEKTI domain is functionally ligated to a SecA domain (SEQ ID NO: 3).

[0050] Secretory Peptides: According to several embodiments, the therapeutic LEKTI domain is ligated in a manner that can function as one or more secretory or transit signals that tag the protein for transport via the secretory pathway. Any secretory signal that facilitates the exit of the LEKTI protein from bacterial cells can be used as a secretory peptide. Non-limiting examples of secretory peptide signals are listed in Table 1 below.

[0051] [Table 1] TIFF0007844102000002.tif36167

[0052] According to some embodiments, the therapeutic LEKTI domain is functionally ligated to one or more signal sequences derived from endogenous proteins of Staphylococcus epidermidis. Non-limiting examples of secretory signal peptides derived from endogenous proteins of Staphylococcus epidermidis are listed in Table 2 below.

[0053] [Table 2] TIFF0007844102000004.tif238170TIFF0007844102000005.tif247170TIFF00078441020 00006.tif227170TIFF0007844102000007.tif230170TIFF0007844102000008.tif233170

[0054] According to several embodiments, the therapeutic LEKTI domain is functionally ligated to one or more secretory signal sequences derived from endogenous proteins of other bacteria. A non-limiting list of secretory signal peptides derived from various bacterial endogenous proteins is provided in Appendix A.

[0055] According to some embodiments, recombinant LEKTI domains are ligated in a manner that can function with a cell permeable peptide sequence that enhances the ability of the LEKTI domain to cross the cell membrane. The term “enhance” used to describe the cell permeable peptide / LEKTI means that the cell permeable sequence improves the passage of the recombinant LEKTI domain across the cell membrane compared to recombinant LEKTI domains lacking the cell permeable sequence.

[0056] Cell-permeable peptides: According to some embodiments, one or more cell-permeable peptides are used to mediate the delivery of therapeutic proteins in vivo without using cell surface receptors and without causing significant membrane damage. According to some embodiments, one or more cell-permeable peptides are ligated to therapeutic proteins in a manner that allows them to function in order to facilitate entry into skin cells (e.g., keratinocytes). Non-limiting examples are listed in Table 3 below.

[0057] [Table 3] TIFF0007844102000010.tif96170

[0058] According to some embodiments, the cell-penetrating peptide comprises a periodic amino acid sequence. Non-limiting examples of periodic cell-penetrating sequences include polyarginine, Rxn (where 4 < n < 17); polylysine, Kxn (where 4 < n < 17); arginine repeats with 6-aminocaproic acid residues in between (RAca) (where there are 2 to 6 arginine repeats); arginine repeats with 4-aminobutyric acid in between (RAbu) (where there are 2 to 6 arginine repeats); arginine repeats with methionine in between (where there are 2 to 6 arginine repeats); arginine repeats with threonine in between (where there are 2 to 6 arginine repeats); arginine repeats with serine in between (where there are 2 to 6 arginine repeats); and arginine repeats with alanine in between (where there are 2 to 6 arginine repeats).

[0059] According to some embodiments, the LEKTI domain is linked in a functional form to the RMR domain (SEQ ID NO: 4).

[0060] According to some embodiments, the expression of the LEKTI domain is controlled by an operon, and the amount of LEKTI provided to mammalian skin is proportional to the availability of external factors. For example, in some embodiments, the recombinant LEKTI gene may be under the control of a xylose-inducible promoter (e.g., a xylose isomerase gene (xylA) containing a xylose repressor (xylR), a xylose operator (xylO), and a cis-acting catabolite-responsive element (CRE)), and the amount of recombinant LEKTI protein made available to mammalian skin is controlled by the amount of exogenous xylose available to the recombinant microorganism. According to some embodiments, the expression of the LEKTI domain is controlled by a promoter that is constitutively active. According to some embodiments, the expression of the LEKTI domain is controlled by the CmR promoter set forth in SEQ ID NO: 8.

[0061] According to several embodiments, microorganisms are genetically modified by transfection / transformation using recombinant DNA plasmids encoding the LEKTI protein domain and one or more antibiotic resistance genes. For example, some embodiments of recombinant DNA plasmids include kanamycin resistance genes and / or trimethoprim resistance genes; e.g., dfrA (SEQ ID NO: 5). According to several embodiments, treatment of mammalian skin with antibiotics (to which the recombinant microorganisms are resistant) can be used to bias the commensal microbial population toward a larger proportion of LEKTI-producing microorganisms. Other elements that may be present in the recombinant DNA plasmid include, but are not limited to, replication protein genes, such as members of the Rep superfamily of replication proteins. For example, in some embodiments, the recombinant DNA plasmid includes the repF gene (SEQ ID NO: 6).

[0062] According to some embodiments, the recombinant DNA plasmid comprises one or more sequences of the pJB38 vector. In some embodiments, recombinant LEKTI is ligated in a manner that allows it to function as an inducible promoter, ribosome binding site, transition signal, and / or cell permeable peptide in the pJB38 vector. As used herein, the term “pJB38-LEKTI-complete” means a recombinant DNA plasmid construct comprising the pJB38 vector and one or more LEKTI domains. According to some embodiments, the recombinant DNA plasmid comprises the pJB38 vector described in SEQ ID NO: 1542. According to some embodiments, the LEKTI domain described in SEQ ID NO: 1 is ligated in a manner that allows it to function in the pJB38 vector described in SEQ ID NO: 1542.

[0063] According to some embodiments, the recombinant DNA plasmid comprises the pKK30-LEKTI complete sequence described in Sequence ID No. 7 (Appendix B). According to some embodiments, the present disclosure provides compositions for the treatment of skin diseases comprising a microorganism comprising a pKK30-LEKTI complete plasmid construct. According to some such embodiments, the microorganism is selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus (e.g., S. epidermidis and / or S. hominis), Lactobacillus (e.g., L. acidophilus), Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[0064] According to some embodiments, the amount or duration of therapeutic LEKTI protein available is controlled by the stability of the LEKTI-carrying vector in the microorganism. For example, the persistence of a recombinant vector can be controlled by one or more elements of the plasmid, such as host-beneficial genes, plasmid stability mechanisms, and those that provide plasmid co-fitting. For example, some plasmids can provide stable replication, active distribution mechanisms, and mechanisms that ensure reliable inheritance of the plasmid to daughter cells over several generations (see, for example, JC Baxter, BE Funnell, Plasmid partition mechanisms, Microbiol. Spectr., 2 (2014) PLAS-0023-2014 and Nils Hulter et al., An evolutionary perspective on plasmid lifestyle modes, Current Opinion in Microbiology, Volume 38, August 2017, Pages 74-80, respectively, the entirety of which is incorporated herein by reference). According to some embodiments, the present invention includes the use of all conventional selection and stabilization methods known to those skilled in the art.

[0065] In one embodiment, the present disclosure provides a method for treating or improving the effects of a mammalian skin disease requiring such treatment, comprising providing a microorganism genetically modified to express one or more LEKTI protein domains on the surface of mammalian skin, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the activity of at least one serine protease in or on mammalian skin. According to some embodiments, the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time and to provide a continuous supply of LEKTI protein domains.

[0066] In another embodiment, the Disclosure provides (1) a composition comprising a microorganism genetically modified to express one or more LEKTI protein domains, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the serine protease activity of at least one serine protease in or on mammalian skin; and (2) a kit for treating or improving the effects of a mammalian skin disease requiring it, comprising reagents for applying the composition to mammalian skin. According to some embodiments, the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time and to result in a continuous supply of LEKTI protein domains.

[0067] In addition to the components described above, the kit in question will further include instructions for the use of the components and / or for carrying out the methods in question. These instructions may be present in the kit in question in various forms, and one or more of these forms may be present in the kit. One form in which these instructions may be present is printed information relating to a preferred medium or substrate, such as a piece of paper or multiple pieces of paper on which the information is printed, in the kit packaging or in the accompanying documentation. Yet another means is a computer-readable medium, such as a diskette or CD, on which the information is recorded. Yet another means in which the instructions may be present is the address of a website used via the Internet to access the information at a transferred site. Any conventional means may be present in the kit.

[0068] The components of the kit can be packaged in an aqueous medium or in a lyophilized form. The kit will generally be packaged to include at least one vial, test tube, flask, bottle, syringe or other container means that can contain and, preferably, suitably ali-coat the described reagents. If additional components are provided, the kit will also generally include a second, third, or other additional container that can contain such components.

[0069] The kits of this disclosure will typically also include means for containing tightly sealed reagent containers for commercial sale. Such containers may include injection or blow-molded plastic containers that hold the desired vials.

[0070] formulation According to several embodiments, a formulation for use according to the present invention comprises a pharmaceutically effective amount of recombinant bacteria producing a therapeutically effective amount of a desired polypeptide or its therapeutically effective domain, for example, at least about 0.01% by weight, about 0.05% by weight, about 0.1% by weight, about 0.2% by weight, about 0.3% by weight, about 0.4% by weight, about 0.5% by weight, about 0.6% by weight, about 0.7% by weight, about 0.8% by weight, about 0.9% by weight, about 1.0% by weight, about 1.5% by weight, about 2.0% by weight, about 3.0% by weight, about 4.0% by weight, and about 5.0% by weight. It may contain recombinant bacteria in amounts of approximately 6.0% by weight, 7.0% by weight, 8.0% by weight, 9.0% by weight, 10.0% by weight, 11.0% by weight, 12.0% by weight, 13.0% by weight, 14.0% by weight, 15.0% by weight, 16.0% by weight, 17.0% by weight, 18.0% by weight, 19.0% by weight, 20.0% by weight, 25.0% by weight, 30.0% by weight, 35.0% by weight, 40.0% by weight, 45.0% by weight, or 50.0% by weight or more, with the upper limit being approximately 90.0% by weight of recombinant bacteria.

[0071] According to some embodiments, formulations for use according to the present invention may contain, for example, at least about 0.01% to about 30% by weight, about 0.01% to about 20% by weight, about 0.01% to about 5% by weight, about 0.1% to about 30% by weight, about 0.1% to about 20% by weight, about 0.1% to about 15% by weight, about 0.1% to about 10% by weight, about 0.1% to about 5% by weight, about 0.2% to about 5% by weight, about 0.3% to about 5% by weight, about 0.4% to about 5% by weight, about 0.5% to about 5% by weight, and about 1% to about 5% by weight or more of recombinant bacteria.

[0072] According to some embodiments, topical formulations may be in any form suitable for application to the body surface, such as creams, lotions, sprays, solutions, gels, ointments, pastes, plasters, coatings, bioadhesives, suspensions, and emulsions, and / or may be prepared to contain liposomes, micelles, and / or microspheres. Such formulations can be used in combination with a closed coating layer such that moisture evaporating from the body surface is maintained in the formulation during and after application to the body surface. According to some embodiments, the formulation may comprise a composition of living cell cultures, and may comprise at least one engineered bacterial strain that produces a therapeutically effective recombinant polypeptide or a therapeutically effective domain thereof. This engineered living cell culture composition can deliver polypeptides directly to the skin to treat or prevent abnormal skin conditions.

[0073] Topical formulations include any other active ingredients dissolved or dispersed in a dermatological vehicle known in the industry (e.g., aqueous or non-aqueous gels, ointments, water-in-oil or oil-in-water emulsions). The components of such a vehicle may be water, aqueous buffer solutions, non-aqueous solvents (ethanol, isopropanol, benzyl alcohol, 2-(2-ethoxyethoxy)ethanol, propylene glycol, propylene glycol monolaurate, glycoflor or glycerol), oils (e.g., mineral oil such as liquid paraffin, natural or synthetic triglycerides such as Miglyol®, or dimethicone, etc.). siliconeIt may also contain oils. In particular, depending on the nature of the formulation and its intended use and application site, the dermatological vehicle used may contain one or more components selected from the following list: solubilizers or solvents (e.g., β-cyclodextrins such as hydroxypropyl β-cyclodextrin, or alcohols or polyols such as ethanol, propylene glycol or glycerol); thickeners (e.g., hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose or carbomer); gelling agents (e.g., polyoxyethylene-polyoxypropylene copolymer); preservatives (e.g., benzyl alcohol, benzalkonium chloride, chlorhexidine, chlorbutol, benzoates, potassium sorbate or EDTA or salts thereof); and pH buffers (e.g., a mixture of dihydrogen phosphate and hydrogen phosphate, or a mixture of citric acid and hydrogen phosphate) (for example, components added to water if the formulation is an aqueous gel).

[0074] Pharmaceutically acceptable carriers may be included in the formulations of the present invention, and these may be any carriers conventionally used in the industry. Examples include water, lower alcohols, higher alcohols, polyhydric alcohols, monosaccharides, disaccharides, polysaccharides, hydrocarbon oils, fats and oils, waxes, fatty acids, silicone Oil, nonionic surfactant, ionic surfactant, siliconeExamples include surfactants, as well as water-based and emulsion-based mixtures of such carriers. As used herein, the terms “pharmaceutically acceptable” or “pharmaceutically acceptable carrier” refer to a compound or composition that can be incorporated into a pharmaceutical formulation without causing undesirable biological effects or undesirable interactions with other components of the formulation, and as used herein, “carrier” or “vehicle” refer to a carrier material suitable for incorporation into a topically applied composition. Useful carriers and vehicles as used herein include any such material known in the art that is non-toxic and does not interact in a harmful manner with other components of the formulation in which it is contained. The term “aqueous” refers to a formulation that contains water or becomes water-containing after application to skin or mucous membrane tissue.

[0075] A film-forming agent forms a protective film on the application site as it dries. This film inhibits the removal of the active ingredient and maintains contact between it and the treated site. An example of a film-forming agent suitable for use in the present invention is Flexible Collodion, US P., described on page 1530 of Remington: The Science and Practice of Pharmacy, 19th edition (Easton, PA: Mack Publishing Co., 1995), where collodion is an ethyl ether / ethanol solution containing pyroxylin (nitrocellulose) which evaporates to release a pyroxylin film. Film-forming agents can also act as carriers. Solutions that dry to form a film are sometimes called coatings. A cream, as is well known in the pharmaceutical industry, is a viscous liquid or semi-solid emulsion of oil in water or water in oil.

[0076] The cream base is washable with water and contains an oil phase, an emulsifier, and an aqueous phase. The oil phase, also called the “internal” phase, typically contains petrolatum and fatty alcohols such as cetyl or stearyl alcohol. The aqueous phase is usually not essential, but it typically exceeds the volume of the oil phase and generally contains a humectant. The emulsifier in the cream formulation is typically a nonionic, anionic, cationic, or amphoteric surfactant.

[0077] A lotion is a preparation applied to the skin surface without friction, and is typically a liquid or semi-liquid preparation in which particles containing an active agent are present in a water or alcohol base. Lotions are usually suspensions of solids, preferably containing an oil-in-water liquid emulsion. Because lotions are easier to apply in larger quantities, they are the preferred formulations herein for treating larger body areas. Insoluble substances in lotions generally need to be finely fragmented.

[0078] The lotion will typically contain suspending agents to provide better dispersion, as well as compounds useful for localizing and retaining active ingredients in contact with the skin, such as methylcellulose, ethoxymethylcellulose sodium, etc.

[0079] A solution is a homogeneous mixture prepared by dissolving one or more chemical substances (solutes) in a liquid such that the molecules of the dissolved substance are dispersed among the molecules of the solvent. A solution may contain other pharmaceutically or cosmetically acceptable chemical substances to buffer, stabilize, or retain the solute. Common examples of solvents used in preparing a solution are ethanol, water, propylene glycol, or any other acceptable vehicle. As is well known, gels are semi-solid suspension systems. Single-phase gels typically contain organic polymers substantially uniformly distributed through a carrier liquid, which is typically aqueous, but preferably alcohol, and optionally oil as well. Preferred “organic polymers,” i.e., gelling agents, are polymers of the “carbomer” family, such as cross-linked acrylic polymers like carboxypolyalkylenes, commercially available under the Carbopol trademark. Also preferred are hydrophilic polymers such as polyethylene oxide, polyoxyethylene-polyoxypropylene copolymer and polyvinyl alcohol; cellulosic polymers such as hydroxypropylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, hydroxypropylmethylcellulose phthalate and methylcellulose; rubbers such as tragacanth and xanthan gum; sodium alginate; and gelatin. To prepare a uniform gel, a dispersant such as alcohol or glycerin may be added, or the gelling agent may be dispersed by grinding, mechanical mixing or stirring, or a combination thereof. Ointments are, as is also well known in the art, typically semi-solid preparations based on petrolatum or other petroleum derivatives. The specific ointment base used provides several desirable characteristics, such as emollient properties, as will be understood by those skilled in the art. Like other carriers or vehicles, the ointment base should be inert, stable, non-irritating, and non-sensitizing.As described in Remington: The Science and Practice of Pharmacy, 19th edition (Easton, PA: Mack Publishing Co., 1995), pages 1399–1404, ointment bases can be classified into four classes: fatty bases; emulsifying bases; emulsion bases; and water-soluble bases. Examples of fatty ointment bases include vegetable oils, animal fats, and semi-solid hydrocarbons derived from petroleum.

[0080] Emulsifying ointment bases, also known as absorbent ointment bases, contain little to no water and include, for example, hydroxystearin sulfate, anhydrous lanolin, and hydrophilic petrolatum.

[0081] Emulsion ointment bases are water-in-oil (W / O) emulsions or oil-in-water (O / W) emulsions, and include, for example, acetyl alcohol, stearyl monostearate, lanolin, and stearic acid. Preferred water-soluble ointment bases are prepared from polyethylene glycol with varying molecular weights; for further information, see Remington: The Science and Practice of Pharmacy.

[0082] A paste is a semi-solid dosage form in which an active agent is suspended in a suitable base. Depending on the properties of the base, pastes are divided into those made from fatty pastes and those made from monophase aqueous gels. The base in fatty pastes is generally petrolatum or hydrophilic petrolatum. Pastes made from monophase aqueous gels generally contain carboxymethylcellulose as the base.

[0083] The accelerator is typically a lipophilic co-accelerator called a “plasticity” accelerator, i.e., an accelerator having a molecular weight in the range of about 150 to 1000, aqueous solubility of less than about 1 wt%, preferably less than about 0.5 wt%, and most preferably less than about 0.2 wt%. The Hildebrandt solubility parameter δ of the plasticity accelerator is in the range of about 2.5 to about 10, preferably in the range of about 5 to about 10. Preferred lipophilic accelerators are fatty esters, fatty alcohols, and fatty ethers. Examples of particular most preferred fatty acid esters include methyl laurate, ethyl oleate, propylene glycol monolaurate, propylene glycol dilaurate, glycerol monolaurate, glycerol monooleate, isopropyl n-decanoate, and octyldodecyl myristate. Examples of fatty alcohols include stearyl alcohol and oleyl alcohol, while examples of fatty ethers include diols or triols, preferably compounds in which a C2-C4 alkanediol or triol is substituted with one or two fatty ether substituents.

[0084] Further penetration enhancers are known to those skilled in the art of topical drug delivery and / or described in relevant textbooks and literature. See, for example, Percutaneous Penetration Enhancers, Smith et al. (eds.) (CRC Press, 1995) (incorporated herein by reference).

[0085] In addition to those identified above, various other additives may be included in the compositions of the present invention. These include, but are not limited to, antioxidants, astringents, fragrances, preservatives, emollients, pigments, dyes, humectants, propellants, and sunscreens, as well as other classes of materials whose presence is pharmaceutically desirable or otherwise desirable. Typical examples of additives as needed for inclusion in the formulation of the present invention are as follows: preservatives such as sorbates; solvents such as isopropanol and propylene glycol; astringents such as menthol and ethanol; emollients such as polyalkylene methyl glucoside; humectants such as glycerin; emulsifiers such as glyceryl stearate, PEG-100 stearic acid, polyglyceryl-3-hydroxylauryl ether, and polysorbate 60; other polyhydroxy alcohols such as sorbitol and polyethylene glycol; sunscreens such as octyl methoxyl cinnamate (commercially available as Parsol MCX) and butyl methoxybenzoylmethane (available under the trademark Parsol 1789); ascorbic acid (vitamin C), α-tocopherol (vitamin E), β-tocopherol, γ-tocopherol, δ-tocopherol, ε-tocopherol, ζ ι - Tocopherol, Ζ Λ - Antioxidants such as tocopherol, η-tocopherol, and retinol (vitamin A); essential oils, ceramides, essential fatty acids, mineral oils, vegetable oils (e.g., soybean oil, coconut oil, liquid fraction of shea butter, sunflower oil), animal oils (e.g., perhydrosqualene), synthetic oils, siliconeOils or waxes (e.g., cyclomethicone and dimethicone), fluorinated oils (generally perfluoropolyethers), fatty alcohols (e.g., cetyl alcohol), and waxes (e.g., beeswax, carnauba wax, and paraffin wax); skin texture modifiers; and thickeners and structuring agents such as cross-linked carboxypolyalkylenes, which can be commercially obtained under the trademarks of Swelling Clay and Carbopol. Other additives include beneficial agents such as materials that tone the skin (especially the upper layers of the skin in the stratum corneum), keep it soft by delaying the decrease in its moisture content, and / or protect the skin. Examples of such conditioners and moisturizers include pyrrolidinecarboxylic acid and amino acids; organic antimicrobial agents such as 2,4,4'-trichloro-2-hydroxydiphenyl ether (triclosan) and benzoic acid; anti-inflammatory agents such as acetylsalicylic acid and glycylretinic acid; anti-seborrheic agents such as retinoic acid; vasodilators such as nicotinic acid; melanin-forming inhibitors such as kojic acid; and mixtures thereof. Further additional active agents include, for example, alpha hydroxy acids, alpha keto acids, polymeric hydroxy acids, moisturizers, collagen, marine extracts, and ascorbic acid (vitamin C), α-tocopherol (vitamin E), β-tocopherol, γ-tocopherol, δ-tocopherol, ε-tocopherol, ζ ιAntioxidants such as α-tocopherol, ζ2-tocopherol, η-tocopherol, and retinol (vitamin A), as well as / or pharmaceutically acceptable salts, esters, amides, or other derivatives thereof. A preferred tocopherol compound is α-tocopherol. Additional agents include, for example, those that can improve oxygen supply to skin tissue, as described in Gross et al. WO94 / 00098 and Gross et al. WO94 / 00109 (incorporated herein by reference), both assigned to Lancaster Group AG. Sunscreens and UV-absorbing compounds may also be included. Non-limiting examples of such sunscreens and UV-absorbing compounds include aminobenzoic acid (PABA), avobenzone, cinoxate, dioxybenzone, homosalate, menthyl anthranilate, octocrylene, octyl methoxycinnamate, octyl salicylate, oxybenzone, padimate O, phenylbenzimidazole sulfate, surisobenzone, titanium dioxide, trolamine salicylate, zinc oxide, ensulizole, melazilate, octinoxate, octisalate, and octocrylene. See Title 21, Chapter 1, Subchapter D, Part 352, “Sunscreen drug products for over-the-counter human use,” which is incorporated herein in its entirety.

[0086] Other embodiments may include a variety of non-carcinogenic, non-irritating healing materials to facilitate treatment with the formulations of the present invention. Such healing materials may include nutrients, minerals, vitamins, electrolytes, enzymes, herbs, plant extracts, glandular extracts or animal extracts, or safe therapeutic agents that can be added to the formulations to facilitate the healing of skin disorders.

[0087] The amounts of these various additives are those conventionally used in the field of cosmetics, ranging, for example, from approximately 0.01% to approximately 20% of the total weight of the topical formulation.

[0088] The formulations of the present invention may also contain conventional additives such as opacifiers, fragrances, colorants, stabilizers, and surfactants. In certain embodiments, other agents such as antimicrobial agents may be added to prevent spoilage during storage, i.e., to inhibit the growth of microorganisms such as yeast and mold.

[0089] Suitable antimicrobial agents are typically selected from the group consisting of methyl and propyl esters of p-hydroxybenzoic acid (i.e., methyl and propylparaben), sodium benzoate, sorbic acid, imidourea, and combinations thereof. In other embodiments, repressors and inducers, i.e., other agents to inhibit (i.e., glycose) or induce (i.e., xylose) the production of the polypeptide of interest, may also be added. Such additives may be used on the condition that they are compatible with the function of the formulation and do not inhibit it.

[0090] The formulation may also contain irritation-reducing additives to minimize or eliminate the potential for skin irritation or skin damage resulting from the administered chemical or other components of the composition.

[0091] Suitable irritation-reducing additives include, for example, α-tocopherol; monoamine oxidase inhibitors, particularly phenyl alcohols such as 2-phenyl-1-ethanol; salicylates; ascorbic acid salts; ionophores such as monensin; amphoteric amines; ammonium chloride; N-acetylcysteine; capsaicin; and chloroquine. If present, irritation-reducing additives can be included in the composition at concentrations effective in reducing irritation or skin damage, typically accounting for about 20 wt% or less of the formulation, and more typically about 5 wt% or less.

[0092] Further preferred pharmacologically active substances that can be incorporated into the formulation in certain embodiments and thus applied topically together with the active agent include, but are not limited to, the following: agents that improve or eliminate pigmented or non-pigmented age spots, keratin, and wrinkles; antimicrobial agents; antibacterial agents; antipruritic and anti-drying agents; anti-inflammatory agents; local anesthetics and analgesics; corticosteroids; retinoids; vitamins; hormones; and antimetabolites.

[0093] Some examples of topical pharmacologically active substances include acyclovir, amphotericin, chlorhexidine, clotrimazole, ketoconazole, econazole, miconazole, metronidazole, minocycline, nystatin, neomycin, kanamycin, phenytoin, para-aminobenzoate, octyl methoxycinnamate, octyl salicylate, oxybenzone, dioxybenzone, tocopherol, tocopherol acetate, selen sulfate, zinc pyrithione, diphenylhydramine, pramoxin, lidocaine, procaine, erythromycin, tetracycline, clindamycin, crotamiton, and hydro Examples include quinones and their monomethyl and benzyl ethers, naproxen, ibuprofen, cromolyn, retinol, retinyl palmitate, retinyl acetate, coal tar, griseofulvin, estradiol, hydrocortisone, hydrocortisone 21-acetic acid, hydrocortisone 17-valeric acid, hydrocortisone 17-butyric acid, progesterone, betamethasone valerate, betamethasone dipropionate, triamcinolone acetonide, fluocinonide, clobetasol propionate, minoxidil, dipyridamole, diphenylhydantoin, benzoyl peroxide, and 5-fluorouracil.

[0094] Creams, lotions, gels, ointments, and pastes can be spread and gently rubbed onto the affected surface. Solutions can be applied in a similar manner, but more typically, they are applied carefully to the affected area using a dropper, swab, or similar tool.

[0095] The application regimen will depend on several easily determinable factors, such as the severity of the condition and its response to initial treatment, but will typically involve continuous application at least once per day. Those skilled in the art will be able to easily determine the optimal dose, method of administration, and rate of repetition of the formulation to be administered. Generally, the formulation of the present invention is intended to be administered at a rate ranging from once or twice per week to a maximum of once or twice per day.

[0096] The pharmaceutical compositions of the present invention comprise one or more active ingredients, e.g., therapeutic agents, in a mixture of one or more pharmaceutically acceptable diluents or carriers and, optionally, one or more other compounds, drugs, components, and / or materials. Regardless of the chosen route of administration, the drugs / compounds of the present invention are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those skilled in the art. See, for example, Remington, The Science and Practice of Pharmacy (21st edition, Lippincott Williams and Wilkins, Philadelphia, Pa.).

[0097] Pharmaceutically acceptable diluents or carriers are well known in the industry (see, for example, Remington, The Science and Practice of Pharmacy (21st edition, Lippincott Williams and Wilkins, Philadelphia, Pa.) and The National Formulary (American Pharmaceutical Association, Washington, DC)), sugars (e.g., lactose, sucrose, mannitol, and sorbitol), starch, cellulose preparations, calcium phosphate (e.g., dicalcium phosphate, tricalcium phosphate, and calcium hydrogen phosphate), sodium citrate, water, aqueous solutions (e.g., saline solution, sodium chloride injection, Ringer's injection, dextrose injection, dextrose and sodium chloride injection, lactated Ringer's injection), alcohols (e.g., ethyl alcohol, propyl alcohol) Coal and benzyl alcohol), polyols (e.g., glycerol, propylene glycol, and polyethylene glycol), organic esters (e.g., ethyl oleate and triglycerides), biodegradable polymers (e.g., polylactide-polyglycolide, poly(orthoester), and poly(anhydride)), elastomer matrices, liposomes, microspheres, oils (e.g., corn oil, germ oil, olive oil, castor oil, sesame oil, cottonseed oil, and peanut oil), cocoa butter, waxes (e.g., suppository waxes), paraffins, silicone Examples include talc, salicylates, and the like. Each pharmaceutically acceptable diluent or carrier used in the pharmaceutical composition of the present invention must be "acceptable" in the sense that it is compatible with the other components of the formulation and is not harmful to the subject. Suitable diluents or carriers for the selected dosage form and intended route of administration are well known in the art, and acceptable diluents or carriers for the selected dosage form and method of administration can be determined using ordinary knowledge in the art.

[0098] The pharmaceutical composition of the present invention may optionally contain additional components and / or materials commonly used in pharmaceutical compositions. These ingredients and materials are well known in the industry and include: (1) fillers or bulking agents such as starch, lactose, sucrose, glucose, mannitol, and silicic acid; (2) binders such as carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, hydroxypropylmethylcellulose, sucrose, and acacia; (3) humectants such as glycerol; (4) disintegrants such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, sodium starch glycolate, cross-linked sodium carboxymethylcellulose, and sodium carbonate; (5) dissolution retarders such as paraffin; (6) absorption enhancers such as quaternary ammonium compounds; (7) humectants such as cetyl alcohol and glycerol monostearate; (8) absorbents such as kaolin and bentonite clay; (9) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycol, and sodium lauryl sulfate; (10) ethoxylated isostearyl (11) Buffering agents such as alcohol, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum methhydroxyl, bentonite, agar and tragacanth; (12) Lactose, lactose, polyethylene glycol, animal fats and vegetable fats, oils, waxes, paraffins, cocoa butter, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicones, bentonites, silicic acid, talc, salicylates, zinc oxide, agar hydroxide Excipients such as luminium, calcium silicates, and polyamide powders; (13) Inert diluents such as water or other solvents; (14) Preservatives; (15) Surfactants; (16) Dispersants; (17) Controlled release agents or absorption retarders such as hydroxypropyl methylcellulose, other polymer matrices, biodegradable polymers, liposomes, microspheres, aluminum monostearate, gelatin, and waxes; (18) Emulsifiers; (19) Adjuvants; (20) Wetting agents; (21) Emulsifiers and suspending agents;(22) Solubilizers and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, oils (especially cottonseed oil, peanut oil, corn oil, germ oil, olive oil, castor oil, and sesame oil), glycerol, tetrahydrofuryl alcohol, polyethylene glycol, and fatty acid esters of sorbitan; (23) Propellantizers such as chlorofluoro hydrocarbons and volatile unsubstituted hydrocarbons, e.g., butane and propane; (24) Antioxidants; (25) Substances that make the preparation isotonic with the blood of the intended recipient, such as sugars and sodium chloride; (26) Thickeners; (27) Coating materials such as lecithin; and (28) Sweeteners, flavorings, colorants, fragrances, and preservatives. Each such component or material must be “acceptable” in the sense that it is compatible with the other components of the preparation and is not harmful to the subject. Suitable ingredients and materials for the selected dosage form and intended route of administration are well known in the industry, and acceptable formulations and materials for the selected dosage form and method of administration can be determined using ordinary knowledge in the industry.

[0099] Dosage forms for topical or transdermal administration include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches, infusions, and inhalations. The active agent / compound can be mixed with a suitable pharmaceutically acceptable diluent or carrier under sterile conditions. Ointments, pastes, creams, and gels may contain excipients. Powders and sprays may contain excipients and propellants.

[0100] Pharmaceutical compositions of the present invention, suitable for parenteral administration, may contain one or more agents / compounds together with one or more pharmaceutically acceptable sterile isotonic aqueous or nonaqueous solutions, dispersants, suspensions or emulsions, or sterile powders, which can be reconstituted into a sterile injectable solution or dispersant immediately before use, which may contain suitable antioxidants, buffers, formulations, and solutes, or suspending agents or thickeners that make the mixture isotonic with the intended recipient's blood. Appropriate fluidity can be maintained, for example, by the use of coating materials, by maintaining the required particle size in the case of dispersions, and by the use of surfactants. These pharmaceutical compositions may also contain suitable adjuvants, such as wetting agents, emulsifiers, and dispersants. It is also desirable to include isotonic agents. Furthermore, extension of absorption of the injectable pharmaceutical form can be achieved by including substances that delay absorption.

[0101] The following embodiments are provided to further illustrate the methods of the present invention. These embodiments are illustrative and are not intended to limit the scope of the present invention in any way. [Examples]

[0102] bacteria In some embodiments, the bacterium Staphylococcus aureus RN4220 strain can be used in vector preparation (Kreiswirth, BN et al., 1983). In some such embodiments, a stock solution of the strain is stored in LB or TS broth in 50% glycerol at -20°C.

[0103] According to some embodiments, the bacterium Staphylococcus epidermidis strain ATCC 12228 or NRRL B-4268 can be used (Zhang, YQ. et al., 2003). In some such embodiments, a stock solution of the strain is stored at -20°C in LB broth or TS broth in 50% glycerol. The bacteria are cultured in LB broth or TS broth. After 16 hours of incubation, the bacteria are harvested by centrifugation and 2 x 10⁶ 9 Concentrate 10-fold in LB broth or TS broth at a concentration of 1 bacteria / 100 μl. Prepare a bacterial stock preparation by inoculating 5 mL of broth with S. epidermidis and grow it overnight at 30°C. Then, add 3 mL of the fully grown culture to 1 mL of 60% glycerol and store at -80°C.

[0104] Expression vector According to some embodiments, the plasmid construct pKK30-LEKTI-complete may include a pKK30 vector along with a LEKTI domain insertion. According to some embodiments, the LEKTI domain can be functionally ligated to a SecA secretion signal, a 6xHis tag, and / or an RMR cell permeable sequence and expressed under the control of a chloramphenicol resistance (CmR) promoter sequence (derived from pDB114E). In some embodiments, the pKK30 vector includes a dihydrofolate reductase (dfrA) selector gene.

[0105] Transformation According to some embodiments, a vector carrying the LEKTI sequence can be used to transform S. epidermidis strains. A vector carrying the LEKTI sequence can be prepared / transformed by a method comprising the steps of preparing competent S. aureus bacterial cells, transforming S. aureus, isolating plasmid DNA from S. aureus, preparing competent S. epidermidis bacterial cells, transforming S. epidermidis, growing the transformed S. epidermidis bacteria, and preserving the transformed S. epidermidis.

[0106] In some embodiments, alternative intermediate strains may also be used for the transformation and isolation of plasmid DNA in the preparation for transformation into S. epidermidis. These strains may include, but are not limited to, other bacteria, including methylation-deficient strains, particularly E. coli strains.

[0107] According to some embodiments, S. aureus RN4220 cells can be made electrocompetent by growing a 50 ml culture overnight in LB or TS medium at 37°C, and then inoculating 10 ml of the overnight culture into 100 ml of fresh LB or TS medium. OD 600 When the level reaches 0.2-0.3, allow the cells to settle and resuspend them in 1x volume of 10% sucrose at 4°C. Repeat this process three times, then resuspend the cells in 0.1x volume of 10% sucrose at 4°C, allow them to settle, and resuspend them in 1 ml of 10% sucrose.

[0108] For the transformation of RN4220, 200-500 μg of LEKTI plasmid (e.g., pKK30-LEKTI-complete) can be mixed with electrocompetent cells and transformed using electroporation at 2.5 kV at room temperature with a MicroPulser Electroporator (Bio-Rad, Hercules, CA). The transformed cells are seeded overnight on selective LB or TB medium at 28°C, grown overnight in selective LB or TB medium, and then used to isolate the DNA.

[0109] According to several embodiments, electrocompetent S. epidermidis ATCC 12228 or NRRL B-4268 is prepared using the following method. First, a 50 ml overnight culture of ATCC 12228 or NRRL B-4268 from a -80°C stock is grown at 37°C in B2 medium (1.0% tryptone, 2.5% yeast extract, 0.5% glucose, 2.5% NaCl, 0.1% K2PO4, pH 7.5). A 10 ml overnight culture is diluted in fresh, pre-warmed B2 medium and OD 600 Shake until the volume reaches 0.5-0.6, then allow to settle at 4°C for 10 minutes. Next, wash the cells with 1, 1 / 2, 1 / 20, and 1 / 50 volumes of cold 10% glycerol, allowing to settle at 4°C between washes. Resuspend the final pellet in 700 μl of cold 10% glycerol.

[0110] According to some embodiments, electrocompetent ATCC 12228 or NRRL B-4268 are transformed using pKK30-LEKTI-complete isolated from S. aureus by electroporation at 2.5kV, 25μF, and 100Ω (a normal reading is 4.5–5 msec using a Micropulser Electroporator (Bio-Rad, Hercules, CA)). The cells are then seeded on selective LB or TB medium at 28°C. In some embodiments, bacterial transformation can also be carried out by alternative transformation methods, including, but not limited to, alternative intermediate strains, bacteriophage transduction, and heat shock.

[0111] Analysis of protein expression According to several embodiments, transformed cells are fractionated and analyzed by SDS-PAGE electrophoresis and Western blotting. Bacterial cells expressing recombinant LEKTI and bacterial control cells are precipitated and lysed with CelLytic B Cell Lysis Reagent (Sigma-Aldrich, St. Louis, MO). The supernatant derived from the induced samples is collected and concentrated. The samples are resuspended in reducing sample buffer and then electrophoresed on a 4-15% Tris-acrylimide gel using Tris-HCl electrophoresis buffer. After electrophoresis, the gel is transferred to a PVDF membrane and sequentially scanned for LEKTI domains 8-11 using primary goat monoclonal antibodies or His tags. Subsequently, horseradish peroxidase conjugate donkey anti-goat antibodies (sc-2020) are scanned, and secondary antibodies are detected by autoradiography (Syngene GeneGnome Bio Imaging System) using an enhanced chemiluminescent substrate (SuperSignal West Pico, Thermo Scientific).

[0112] Analysis of the supernatant and cell lysates demonstrates the successful expression and secretion of the therapeutic polypeptide during transformation using a plasmid containing the target protein. Detection of protein expression and secretion may also be possible using alternative methods, and this example should not be construed as a limitation to the present invention.

[0113] Treatments for Humans According to some embodiments, 1 x 10 9 Colony-forming units (CFUs) of S. epidermidis can be added to a pharmaceutically acceptable carrier. The composition is useful for treating or preventing abnormal skin conditions resulting from Netherton syndrome in subjects requiring it. The composition can be applied at least once a day, for example, up to about 3-4 times a day, or as needed, or as prescribed. In some embodiments, only one application is required to achieve a therapeutic effect. The composition can be used for as long as necessary to ensure treatment of the condition or to continue preventing the condition. The duration of treatment may vary from about 1 day to a maximum of about 10-14 days, or longer. In certain cases, long-term or chronic treatment may be administered. [Examples]

[0114] Testing of the serine protease inhibitory activity of recombinant LEKTI According to several embodiments, the protease inhibitory activity of recombinant LEKTI is tested for differences achieved when it is functionally linked to various secretory and cell-permeable peptides. According to some embodiments, certain combinations of secretory and cell-permeable peptides may have unpredictable effects on the protease inhibitory function of the LEKTI domain and may therefore be determined empirically.

[0115] In some embodiments, LEKTI domains D8–D11, ligated in a manner capable of functioning as secretory tags, 6xHis tags, and / or cell permeability tags, are cloned into insect expression vectors for large-scale production of purified recombinant proteins and evaluated for their inhibitory activity against one or more proteases (e.g., plasmin, cathepsin G, elastase, and trypsin).

[0116] Insect cells and reagents The following reagents can be commercially obtained as indicated: fall armyworm cell line Spodoptera frugiperda (Sf9), low-melting point agarose, cellFECTIN, pFASTBAC1, pCRII-TOPO, competent Escherichia coli DH10BAC, cabbage looper egg cell line Trichoplusia ni 5B1-4 (High Five), and ultimate serum-free insect medium from Invitrogen (Carlsbad, CA); restriction endonuclease from New England Biolabs (Beverly, MA); TALON Superflow from Clontech Laboratory (Palo Alto, CA); Insect-XPRESS medium and fetal bovine serum from BioWhittaker (Walkersville, MD); YM10 Centriplus from Millipore Corp. (Bedford, MA); precast SDS-PAGE gels, protein assay kits, SEC-250 size columns, and Bio-Rad Pre-staining markers from Hercules (CA); BSA from Kabi Pharmacia (Franklin, OH); DTT and glycerol from Boehringer Mannheim Biochemicals (Indianapolis, IN); and 5xHis mAb and 6xHis-tagged protein ladder from QIAGEN Inc. (Valencia, CA).

[0117] Cloning and expression of LEKTI D8-D11 A 6xHis-tagged LEKTI domain (e.g., Sequence ID No. 1), ligated in a manner capable of functioning with various permutations of secretory and cell-permeable peptides, can be cloned into the pFASTBAC1 vector according to the manufacturer's instructions. Recombinant LEKTI-complex viruses are then generated, as previously described by Gao, M. et al., (1996) J. Biol. Chem. 271, 27782-27787, which is incorporated herein by reference in its entirety. To test the recombinant LEKTI-complex viruses for recombinant LEKTI expression, Sf9 cells can be infected with the recombinant virus at various infection multiplicities, and cell lysates and media can be collected every 24–96 hours. The presence of histidine-tagged proteins can be confirmed by Western blotting analysis using a 5xHis mAb against a 6x histidine tag, according to the manufacturer's recommendations. The LEKTI-complex viruses showing the highest levels of expression can be selected for further experiments and spinner flasks.

[0118] Recombinant LEKTI protein can be produced on a large scale by infecting spinner cultures of Sf9 cells (1.6 billion cells) in Insect-XPRESS medium containing 10% serum with an infection multiplicity of 8 plaque-forming units (PFUs). Three days after infection, the cell pellet is harvested as previously described in Jayakumar, A. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 8695-8699, and Co 2+ Recombinant LEKTI can be selectively purified from cell lysates using a charged Sepharose affinity column (TALON), followed by SEC-250 size column chromatography. Fractions containing homogeneous LEKTI can be pooled and concentrated by ultrafiltration. The protein can then be quantified using the Bio-Rad Protein Assay Kit II.

[0119] Reagents and protocols for protease inhibition assays The following enzymes, chromogenic substrates, and reagents can be commercially obtained as indicated: human plasmin, human cathepsin L, human cathepsin S, human trypsin, human cathepsin G, human chymotrypsin, and human neutrophil elastase (HNE) from Athens Research & Technology, Inc. (Athens, GA); subtilisin A from Calbiome-Novabiochem (San Diego, CA); papain from Roche Molecular Biochemicals (Indianapolis, IN); furin from New England BioLabs; succinyl-Ala-Ala-Pro-Phe-p-nitroanilide (Succ-AAPF-pNA), succinyl-Ala-Ala-Val-pNA (Succ-AAVpNA), and D-Val-Leu-Lys-pNA (VLK-pNA) from Sigma Chemical Co. (St. Louis, MO); and Bachem Bioscience, Inc. (King of H-Glu-Gly-Arg-pNA (EGRpNA) and benzyloxycarbonyl-Phe-Arg-pNA (Z-FR-pNA) from Prussia, PA; and methoxy-Succ-Arg-Pro-Tyr-pNA (MeO-Succ-RPY-pNA) from Chromogenix Instrumentation Laboratory SpA (Milan, Italy). PBS reaction buffer (137 mM NaCl, 27 mM KCl, and 10 mM phosphate buffer (pH 7.4)) can be used with trypsin, plasmin, cathepsin G, HNE, and chymotrypsin. Cathepsin reaction buffer (0.1% CHAPS, 50 mM sodium acetate (pH 5.5), 1 mM EDTA) can be used with cathepsin K, L, and S, as well as papain. A unique reaction buffer can be used with subtilisin A (PBS and 0.1% Tween 20).

[0120] The ability of recombinant LEKTI to block cleavage of small chromogenic peptide substrates, determined by the spectroscopic techniques previously described in Schick, C. et al. (1998) Biochemistry 37, 5258-5266, which is incorporated herein by reference in its entirety, allows for the detection of protease inhibitory activity. Protease inhibition can be evaluated after pre-incubating the enzyme with recombinant LEKTI for 2 minutes at 25°C in 100 μL of assay buffer. This mixture can be added to 890 or 880 μL of assay buffer in a 1 mL quartz cuvette. Protease activity can be initiated by adding 10 - 20 μL of the appropriate pNA substrate. The change in absorbance at 405 nm (A 405 = 8.8 10 -3 M -1 cm -1 ) can be followed over 10 minutes using a spectrophotometer (Beckman Instruments, Inc., Fullerton, CA). The rate changes (ΔA405 / min) of the inhibited and control reactions can be determined from the rate plots.

[0121] According to some embodiments, different combinations of secretion tags and cell-penetrating tags can elicit different LEKTI protease activities against each of the proteases being tested (e.g., trypsin, plasmin, cathepsin G, HNE, subtilisin A, and chymotrypsin). Additionally, individual combinations of secretion tags and cell-penetrating tags can elicit different LEKTI protease activities among individual proteases.

Example

[0122] Transduction Peptide-Mediated Delivery According to some embodiments, various combinations of secretion tags and cell-penetrating tags can affect, more or less, the ability of the recombinant LEKTI protein to cross the cell membrane. Thus, various recombinant LEKTI products can be tested in cell culture to evaluate the effects of various combinations of secretion tags and cell-penetrating tags.

[0123] According to some embodiments, adherent fibroblast cell lines HS-68, NIH-3T3, 293, Jurkat T, or Cos-7 can be cultured at 37°C in a humidified atmosphere containing 5% CO2 in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1% (vol / vol) 200 mM glutamine, 1% (vol / vol) antibiotics (streptomycin, 10,000 μg / ml; penicillin, 10,000 IU / ml), and 10% (wt / vol) FBS. For peptide-mediated delivery of recombinant LEKTI protein, purified recombinant LEKTI product (obtained above) can be loaded into DMEM or PBS (500 μl of DMEM containing 0.25 μg of protein) and incubated at 37°C for 30 minutes. Cells then grown to a 75% concentration density are coated with these recombinant LEKTI protein media. After incubation at 37°C for 30 minutes, without removing the recombinant LEKTI protein overlay, 1 ml of fresh DMEM supplemented with 10% FBS is added to the cells, and the cells are returned to the incubator for another 30 minutes. The cells are then thoroughly washed with PBS and examined for recombinant LEKTI protein. The cells can be observed by immunofluorescence by first fixing with 2% formalin (Sigma), permeabilizing, and then incubating with primary anti-6x His-tagged antibody and secondary antibody according to the manufacturer's instructions. Alternatively, cell lysates can be obtained as described above, and the presence of His-tagged recombinant LEKTI can be observed by Western blotting.

[0124] According to some embodiments, specific combinations of secretory proteins and permeable peptides have different effects on the ability of recombinant LEKTI proteins to cross the cell membrane. [Examples]

[0125] The LEKTI protein requires proteolytic cleavage to activate its inhibitory function against many proteases. The full-length protein is cleaved into domains D1–D5 and D6–D15. The D6–D15 domain is then further cleaved in multiple steps into D6–D9 and D10–D15, →D6 and D7–D9→D7 and D8–D9→D8. A schematic diagram of the full-length LEKTI polypeptide, domains, and native cleavage products is shown in Figure 3. In selecting the specific domain to express, the following criteria were considered: (1) activity against various kallikrein-related peptidases (KLK) such as KLK5 and KLK7; (2) protease resistance; (3) small size (not a metabolic burden); and (4) minimal disulfide bond content. Domain 6 was selected as the LEKTI fragment to express. The amino acid sequence of the full-length LEKTI protein is given as SEQ ID NO: 103, and each of the following 15 individual domains is described in fasta format.

[0126] LEKTI amino acid sequence residues 1-1064 (SEQ ID NO: 103): TIFF0007844102000011.tif91165TIFF0007844102000012.tif55164

[0127] The LEKTI domains are listed below: TIFF0007844102000013.tif176166TIFF0007844102000014.tif243166TIFF0007844102000015.tif31166

[0128] The LEKTI nucleic acid sequence is described below as sequence number 119. LEKTI full-length nucleic acid sequence (SEQ ID NO: 119)

[0129] TIFF0007844102000016.tif191168TIFF0007844102000017.tif245166

[0130] Solubility in E. coli BL21(De3) Prokaryotes produce soluble and inclusion body-binding proteins. Solubility is influenced by temperature, protein charge, and protein structure and size. Insoluble inclusion body-binding proteins are often misfolded, are typically inactive, and are isolated within very pure, insoluble inclusion bodies. Inclusion body-binding proteins are isolated in vitro, refolded, and then purified. Soluble proteins are in their folded structure, are often functional, and reside in the cytoplasm along with the rest of the proteome.

[0131] To determine whether domain 6 is reliably produced in E. coli, a first set of experiments was performed. Soluble proteins were isolated and purified by affinity purification and buffer exchange. Solubility assays were used to determine the distribution between inclusion body proteins and the soluble protein fraction. Briefly, cells expressing domain 6 protein (E. coli BL21(De3)) were lysed with aqueous buffer. The soluble and inclusion body fractions were isolated using high-speed centrifugation and inclusion body purification. The isolated fractions were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Figure 4 shows the SDS-PAGE results indicating that LEKTId6 (8.8 kDa) is highly soluble in E. coli BL21(De3). E. coli GFP (33.8 kDa) was used as a positive control, and no vector was used as a negative control. Experiments were performed at three different induction temperatures: 18°C, 30°C, and 37°C. As shown in Figure 4, an 8.8 kDa band was detected in the soluble fraction of the His6 LEKTId6 experimental group. The arrow indicates the 8.8 kDa band.

[0132] Figure 5 shows the SDS-PAGE results indicating that affinity purification was successfully performed for H6-LEKTId6 (8.8 kDa). The arrow indicates the 8.8 kDa band. Figure 6 shows that LEKTId6-H6 (8.8 kDa) can be N-terminally truncated. In both Figures 5 and 6, the following abbreviations were used for the experimental group: SN = Cleared cell lysate (supernatant). FT = Non-Ni2+ binding protein (leachate). W1-4 = Eluents from a series of washes (1-4). Note some contamination from neighboring ladders in W4. L = SDS-PAGE protein ladder (SeeBlue Plus2, ThermoFisher Scientific). E1-6 = Eluents from the column after imidazole treatment (i.e., the resulting affinity-purified proteins). Different eluent fractions (1-6) were collected when the column was treated. [Examples]

[0133] We evaluated the ability of purified recombinant LEKTI domain 6 (LEKTId6) fragments to function in vitro as serine protease inhibitors.

[0134] First, the ability of recombinantly produced LEKTId6 to inhibit trypsin in vitro was determined. Enzyme activity was measured using BApNA (Nα-benzoyl-1-arginine-p-nitroanilide) as a trypsin-specific substrate. Figure 7A shows a schematic diagram of the assay. The assay was performed by mixing 80 μL of LEKTId6 at concentrations of 0.25, 2.5, and 25 μM with 20 μL of trypsin (35 μg / mL) and 100 μL of 2x trypsin assay buffer (100 mM Tris-HCl, pH 8.0, 300 mM NaCl, 100 mM CaCl2, 0.02% Triton X-100, 500 μM L-BAPNA). In the reaction mixture, the final concentrations were LEKTId6 (0.1, 1, 10 μM); trypsin (3.5 μg / mL); assay buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 50 mM CaCl2, 0.01% Triton X-100); and L-BAPNA (250 μM). The reaction was allowed to proceed at 37°C for 15 minutes. Leupeptin, a trypsin inhibitor, was used as a positive control. Product formation was measured at 405 nm using a microplate reader. A blank control was used. Trypsin activity was defined as the rate of change in absorbance at 405 nm per minute (an indicator of L-BAPNA cleavage) under established conditions. As shown in Figure 7, LEKTI domain 6 inhibited trypsin activity in vitro.

[0135] Next, the effect of LEKTI domain 6 (ct Hist tag) on ​​trypsin inhibition was determined and compared with the effect of LEKTI domains 10-15 on trypsin inhibition. A trypsin inhibition assay was performed as described above, measuring enzyme activity using L-BAPNA (Nα-benzoyl-1-arginine-p-nitroanilide) as a trypsin-specific substrate. Figure 8 shows a schematic diagram of the assay. LEKTId6 (10, 30, 100, 1000 nm) or LEKTI domains 10-15 (10, 30, 100 nm) were mixed with L-BAPNA (final concentration 250 μM) at 25°C for 10 minutes. Leupeptin, a trypsin inhibitor, was used as a positive control. The ability of recombinantly produced LEKTId6 to inhibit kallikrein 7 and 5 (KRK7 and KRK5) in vitro was determined. Briefly, proteinases KLR7 and KLK5 were incubated with increasing concentrations of LEKTId6 at 25°C for 5 minutes, followed by the addition of their respective optimal peptide substrates: Suc-Arg-Pro-Tyr-p-nitro-anilide for KLK7 and D-Ile-Pro-Arg-p-nitro-anilide for KLK5. Product formation was measured at 405 nm using a microplate reader. A blank control was used. Schematic diagrams of the KLK7 and KLK5 assays are shown in Figures 9A and 10A, respectively. For KLK7, increasing concentrations of LEKTId6 (10, 30, 100, 300, 1000 nm) and increasing concentrations of LEKTId10-15 (10, 30, 100 nm) were used. The trypsin inhibitor leupeptin was used as a negative control. For KLK5, increasing concentrations of LEKTId6 (10, 30, 100, 300, 1000 nm) were used. As shown in Figure 9B, recombinant LEKTI domain 6 inhibits KLK7 in vitro, much like LEKTI domains 10-15. As shown in Figure 10B, recombinant LEKTId6 inhibits KLK5 in vitro at nanomolar concentrations.High concentrations of LETKId6 have been shown to be irritating, but this is not theoretically constrained, and may be due to the buffer components of the assay, particularly excess imidazole remaining in the LETKId6 sample after affinity purification. [Examples]

[0136] The efficacy of the therapeutic LEKTId6 S. epidermidis strain will be evaluated in a conditioned Netherton mouse model. Briefly, the inventors will verify the absence of LEKTI in the skin of Netherton syndrome mice (conditional SPINK5- / -) created by CRISPR after Cre recombination induction at weeks 1, 2, and 4. Mice with verified Netherton syndrome phenotype will be treated with topical application of recombinant LEKTI to resolve the skin condition present in the Spink5 conditional mutants. Firstly, the rationale for using purified LEKTI is to avoid dependence on the S. epidermidis strain construct, allowing the inventors to rapidly demonstrate the efficacy of topical application in vivo. Secondly, the inventors will evaluate the ability of purified S. epidermidis or LEKTI to demonstrate the value of probiotic encombination for sustained improvement. As a control, the inventors will topically encombine pre-Cre induction of SPINK5 conditional mutants in the same mice. To evaluate the effects of LEKTId6 in a mouse model, the inventors performed long-term assays (1x / week) and endpoint assays (3 weeks after fixation), where possible, to test whether the application of therapeutic S. Epidermidis resulted in (1) in vivo detection of LEKTI as measured by immunohistochemical analysis of the skin (endpoint), (2) reduced severity of skin disease as measured by DASI (long-term and endpoint), (3) improvement of TEWL (long-term) and permeability scores (endpoint), (4) improvement of skin morphology as measured by histological analysis (endpoint), and (5) changes in protein lysis activity as measured using colorimetric assays targeting KLK5 and KLK7 (endpoint).

[0137] Embedding by reference The entire disclosure of each patent document, including patent application documents, scientific papers, government reports, websites, and other references listed herein, is incorporated herein by reference in its entirety for any purpose. Any inconsistencies in terminology shall be governed by this Specified Publication. All sequence listings or sequence numbers disclosed herein are incorporated herein by reference in their entirety.

[0138] The following references are incorporated herein by reference to the extent that they provide exemplary procedures or other details that supplement those described herein.

[0139] Armengot-Carbo, M. et al. (2014) "The role of filaggrin in the skin barrier and disease development." Actas Dermosifiliogr Mar; 106 (2):86-95. Brachkova, MI, P. Marques, J. Rocha, B. Sepodes, MA Duarte and JF Pinto (2011). "Alginate films containing Lactobacillus plantarum as wound dressing for prevention of infection." J Hosp Infect 79(4): 375-377. Brown, SJ., & McLean, WH. (2012) J. Invest. Dermatol. 132, 751-62 Chen, YE., & Tsao, H. (2013) J. Am. Acad. Dermatol. 69, 143-155 Cheung AL, et al. (2004) "Regulation of virulence determinants in vitro and in vivo in Staphylococcus aureus." FEMS Immunological Medical Microbiology 40(1): 1-9 "DNA Recombination." Methods in Molecular Biology 745(XIV): 1-565. Gross, et al, WO 94 / 00098, assigned to Lancaster Group AG Gross, et al, WO 94 / 00109, assigned to Lancaster Group AG Gueniche, A., P. Bastien, J. M. Ovigne, M. Kermici, G. Courchay, V. Chevalier, L. Breton and I. Castiel-Higounenc (2010). "Bifidobacterium longum lysate, a new ingredient for reactive skin." Exp Dermatol 19(8): 1-8. Jeong JG et al. (2011). A Tat- grafted anti-nucleic acid antibody acquires nuclear-localization property and a preference for TAR RNA. Biochem Biophys Res Commun. Mar 18;406(3):403-7. Kreiswirth, BN., et al. (1983). The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305:709-712. Lauderdale, et al. (2010). Biofilm dispersal of community-associated methicillin-resistant Staphylococcus aureus on orthopedic implant material. J. Orthop. Research. 28:55-61 Lee, SH., Jeong, SK. and Ahn, SK. (2006). "An update of the defensive barrier function of skin." Yonsei Med J 47(3): 293-306. Lin, YT., Wang, CT., and Chiang, BL. (2007). "Role of bacterial pathogens in atopic dermatitis." Clin Rev Allergy Immunol 33(3): 167-177. Ma, J., et a / .(2014) Cell-penetrating peptides mediated protein cross -membrane delivery and its use in bacterial vector vaccine. Fish & Shellfish Immunology 39 8-16 McAleer, MA., & Irvine, AD. (2013) J. Allergy Clin. Immunol. 131, 280-91. Mitsudo K. et al., (2003) “Inhibition of Serine Proteinases Plasmin, Trypsin, Subtilisin A, Cathepsin G, and Elastase by LEKTI: A Kinetic Analysis”, Biochemistry, 42, 3874-3881 Monk, I., et al. (2012) Direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. mBio. Muizzuddin, N., Maher, W., Sullivan, M., Schnittger, S., and Mammone, T. (2012). "Physiological effect of a probiotic on skin." J Cosmet Sci 63(6): 385-395. Nakanishi, N., T. Oshida, S. Yano, K. Takeda, T. Yamaguchi and Y. Ito (1986). "Construction and characterization of new cloning vectors derived from Streptomyces griseobrunneus plasmid pBTl and containing amikacin and sulfomycin resistance genes." Plasmid 15(3): 217-229. Nakatsuji, T. and R. L. Gallo (2014). "Dermatological therapy by topical application of non-pathogenic bacteria." J Invest Dermatol 134(1): 11-14. Oehike J et al. (1998).Cellular uptake of an alpha-helical amphipathic model peptide with the potential to deliver polar compounds into the cell interior non-endocytically. Biochim Biophys Acta. Nov 11; 1414(1-2): 127-39. Ostenson CG et al. (1997).Galparan: a powerful insulin-releasing chimeric peptide acting at a novel site. Endocrinology . Aug;138(8):3308-13. Otsuka, A., et al. (2014) J. Allergy Clin. Immunol. 133, 139-46.el-10 (2014). Peral, M. C, M. A. Martinez and J. C. Valdez (2009). "Bacteriotherapy with Lactobacillus plantarum in burns." Int Wound J 6(1): 73-81. Peral, M. C, M. M. Rachid, N. M. Gobbato, M. A. Huaman Martinez and J. C. Valdez (2010). "Interleukin-8 production by polymorphonuclear leukocytes from patients with chronic infected leg ulcers treated with Lactobacillus plantarum." Clin Microbiol Infect 16(3): 281-286 Powers, ME., et al. (2011). J Bacteriol, 193:340-348 Proksch, E., J. M. Brandner and J. M. Jensen (2008). "The skin: an indispensable barrier." Exp Dermatol 17(12): 1063-1072 Remington: The Science and Practice of Pharmacy, 19th edition. Easton, PA: Mack Publishing Co., 1995 Sambrook J, et al. (1989). Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory Press, New York. Sambrook, JF., and Russell, DW., ed. (2001). Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1, 2 and 3. Cold Spring Harbor Laboratory Press Simonen, M. and I. Palva (1993). "Protein secretion in Bacillus species." Microbiol Rev 57(1): 109-137 Smith, EW., & Maibach, HI., (1995) Percutaneous Penetration Enhancers, CRC Press ISBN 9780849321528 Stout, TE., et al .(2014) / Invest Dermatol. 134, 423-9 The Science and Practice of Pharmacy (1995), 19th Ed. Easton, PA: Mack Publishing Co. Volz, T., Y. Skabytska, E. Guenova, K. M. Chen, J. S. Frick, C. J. Kirschning, S. Kaesler, M. Rocken and T. Biedermann (2014). "Nonpathogenic bacteria alleviating atopic dermatitis inflammation induce IL- 10 -producing dendritic cells and regulatory Trl cells." J Invest Dermatol 134(1): 96-104 Webb, TR., & Hsu, CPS. US Patent. No. 4,659,774 assigned to American Hoechst Corporation Wyman TB, et al. (1997) Design, synthesis, and characterization of a cationic peptide that binds to nucleic acids and permeabilizes bilayers. Biochemistry. March 11;36(10):3008-17 Zhang, YQ., et al. (2003). Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC12228). Molecular Microbiology 49(6), 1577-1593

[0140] While exemplary embodiments of the present invention have been described herein, it should be understood that the present invention is not limited thereto and that various other modifications or alterations can be made without departing from the scope or spirit of the invention by those skilled in the art. This disclosure includes the following embodiments. [1] A composition for the treatment of skin diseases, This invention includes a microorganism genetically modified to express and result in one or more LEKTI protein domains on mammalian skin, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the serine protease activity of at least one serine protease in or on mammalian skin. composition. [2] The composition according to Embodiment 1, wherein the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time to provide a continuous supply of LEKTI protein domains. [3] The composition according to Embodiment 1, wherein the LEKTI protein domain is effective in improving the symptoms of Netherton syndrome. [4] The composition according to Embodiment 1, wherein the microorganism is genetically modified by transfection / transformation using a recombinant DNA plasmid encoding the LEKTI protein domain. [5] The composition according to Embodiment 1, wherein the LEKTI domain is functionally linked to one or more recombinant protein domains that are effective in enhancing secretion from microorganisms and / or permeability through mammalian skin. [6] The composition according to Embodiment 1, wherein at least one LEKTI domain is functionally linked to a SecA domain. [7] The composition according to Embodiment 1, wherein at least one LEKTI domain is functionally linked to an RMR domain. [8] The composition according to Embodiment 1, wherein at least one LEKTI domain comprises the amino acid sequence described in Sequence ID No. 1. [9] The composition according to Embodiment 1, wherein the microorganisms are adapted to grow on the skin of mammals.

[10] The composition according to Embodiment 1, wherein the expression of at least one LEKTI domain is controlled by an operon, and the amount of LEKTI delivered to mammalian skin is proportional to the availability of an external factor.

[11] The composition according to Embodiment 1, wherein the expression of at least one LEKTI domain is controlled by a constitutively active promoter.

[12] The composition according to Embodiment 1, wherein the microorganism is genetically modified by transfection / transformation using a recombinant DNA plasmid encoding one or more LEKTI protein domains and one or more antibiotic resistance genes.

[13] The composition according to Embodiment 1, wherein the microorganism is selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[14] A method for treating or improving the effects of a mammalian skin disease that requires the same, The present invention provides a genetically modified microorganism that expresses one or more LEKTI protein domains on the surface of mammalian skin, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the activity of at least one serine protease in or on mammalian skin. method.

[15] The method of Embodiment 14, wherein the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time to provide a continuous supply of LEKTI protein domains.

[16] A kit for the treatment or improvement of the effects of a skin disease in a mammal requiring treatment or improvement of the effects of a skin disease, (1) A composition comprising a microorganism genetically modified to express one or more LEKTI protein domains, wherein the LEKTI protein domains are effective in penetrating one or more layers of mammalian skin and are effective in inhibiting the serine protease activity of at least one serine protease in or on mammalian skin; and (2) Reagent for applying the composition to the skin of a mammal A kit that includes this.

[17] The kit according to Embodiment 16, wherein the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time to provide a continuous supply of LEKTI protein domains.

[18] A composition for the treatment of skin diseases comprising a microorganism containing the pJB38-LEKTI complete plasmid construct.

[19] The composition according to Embodiment 18, wherein the microorganism is selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus, and mixtures thereof.

[20] A composition comprising the complete pJB38-LEKTI plasmid construct.

[21] The composition according to Embodiment 20, wherein the pJB38-LEKTI complete plasmid construct is expressed in a microorganism selected from the group consisting of Bifidobacterium, Brevibacterium, Propionibacterium, Lactococcus, Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Pediococcus, Leuconostoc, or Oenococcus and mixtures thereof.

[22] Recombinant microorganism capable of secreting polypeptides, comprising an expression vector comprising a first coding sequence containing a gene capable of expressing polypeptides and a second coding sequence containing a gene capable of expressing cell-permeable peptides.

Claims

1. A composition for the treatment of a skin disease, wherein the skin disease is Netherton syndrome, The composition comprises a microorganism genetically modified to express and produce a polypeptide consisting of LEKTI domain 6 on mammalian skin. The aforementioned microorganism is Staphylococcus epidermidis. The LEKTI domain 6 has the amino acid sequence of SEQ ID NO: 109, composition.

2. The composition according to claim 1, wherein the LEKTI domain 6 consists of the amino acid sequence of SEQ ID NO:

109.

3. The composition according to claim 1, wherein the microorganism is genetically modified by transfection / transformation using a recombinant DNA plasmid encoding LEKTI domain 6.

4. The composition according to claim 1, wherein the LEKTI domain 6 is functionally linked to one or more recombinant protein domains that are effective in enhancing secretion from microorganisms and / or permeability through mammalian skin.

5. The composition according to claim 1, wherein the LEKTI domain 6 is functionally linked to the SecA domain.

6. The composition according to claim 1, wherein the LEKTI domain 6 is functionally linked to the RMR domain.

7. The composition according to claim 1, wherein the expression of LEKTI domain 6 is controlled by an operon, and the amount of LEKTI delivered to mammalian skin is proportional to the availability of external factors.

8. The composition according to claim 1, wherein the expression of LEKTI domain 6 is controlled by a constitutively active promoter.

9. The composition according to claim 1, wherein the microorganism is genetically modified by transfection / transformation using a recombinant DNA plasmid encoding LEKTI domain 6 and one or more antibiotic resistance genes.

10. A kit for treating or improving the effects of a skin disease in a mammal requiring treatment or improvement of the effects of the skin disease, wherein the skin disease is Netherton syndrome, (1) A composition comprising a microorganism genetically modified to express a polypeptide consisting of a LEKTI domain 6, wherein the LEKTI domain 6 has the amino acid sequence of SEQ ID NO: 109, and the microorganism is Staphylococcus epidermidis; and (2) Reagent for applying the composition to the skin of a mammal A kit that includes this.

11. The kit according to claim 10, wherein the LEKTI domain 6 consists of the amino acid sequence of SEQ ID NO:

109.

12. The kit according to claim 10, wherein the microorganism is adapted to survive on the surface of mammalian skin for a controlled period of time to provide a continuous supply of LEKTI domain 6.

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