Particle sorting kit

Abstract

The objective of the present invention is to provide a particle fractionating kit provided with a filter portion with which there is little particle loss in a connecting part between a tube through which a sample liquid flows and the filter portion, and which functions even when the flow rate is low.　This particle fractionating kit is provided with a sample accommodating portion for accommodating a sample liquid containing particles, a sample flow passage through which the sample liquid flows, a detection area in which detection of target particles from within the sample liquid is performed, and a filter portion including a filter and a mating portion for externally mating with a tube for connecting to the sample accommodating portion and / or the sample flow passage, wherein a protruding portion which protrudes toward the tube is provided in the mating portion.

Description

Technical Field

[0001] The present technology relates to a particle separation kit used for separating particles using a microchannel.

Background Art

[0002] Currently, a technique called flow cytometry is used for the analysis of microparticles such as cells and microorganisms. This flow cytometry is an analytical method that irradiates light on microparticles flowing so as to be enclosed in a sheath flow sent in a channel, and analyzes and separates the microparticles by detecting fluorescence and scattered light emitted from each microparticle. The device used for this flow cytometry is called a flow cytometer.

[0003] In this flow cytometer, a microchip provided with regions and channels for performing chemical or biological analysis on a substrate made of silicon or glass is used. An analysis system using such a microchip is called μ-TAS (micro-total-analysis system), lab-on-a-chip, biochip, etc.

[0004] As an application example of μ-TAS to microparticle measurement technology, there is a microparticle separation device that optically, electrically, or magnetically measures and separates the characteristics of microparticles in channels and regions arranged on a microchip. In a flow cytometer (microchip type flow cytometer) applying such μ-TAS, there is an advantage that cross-contamination of samples between measurements can be prevented by configuring a flow path system with a microchip.

[0005] For example, Patent Document 1 discloses a microchip comprising "a main channel through which a liquid containing fine particles flows, a capture chamber where the fine particles are taken in, and a pressure chamber where negative pressure is generated, and a preparative channel communicating with the main channel, wherein the cross-section perpendicular to the liquid flow direction in the capture chamber and the pressure chamber is formed to be larger than the cross-section perpendicular to the liquid flow direction in other parts of the preparative channel." [Prior art documents] [Patent Documents]

[0006] [Patent Document 1] Japanese Patent Publication No. 2017-058375 [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] When analyzing and separating minute particles such as cells and microorganisms, the presence of foreign matter such as particle aggregates and fibrous debris in the sample solution can reduce analytical and separation accuracy. Therefore, it is desirable to remove these foreign matter before passing the sample solution through the microchannel. Furthermore, since the sample solution passed through the microchannel is small in volume and at low flow rates, the filter used to remove foreign matter must have minimal particle loss and function even at low flow rates.

[0008] However, there were problems such as gaps forming at the connection point between the tube through which the sample liquid flows and the filter section, resulting in dead volume, and poor tube retention at the connection point, which reduced work efficiency.

[0009] Therefore, the main objective of this technology is to provide a particle sorting kit equipped with a filter that minimizes particle loss at the connection point between the tube through which the sample liquid flows and the filter, and that functions even at low flow rates. [Means for solving the problem]

[0010] This technology first includes a sample containment section for containing a sample liquid containing particles, A sample channel through which the aforementioned sample liquid flows, A detection region in which target particles are detected from the aforementioned sample liquid, A filter section comprising a filter and a fitting section for outer diameter fitting with a tube for connecting to the sample storage section and / or the sample flow path, Equipped with, The present invention provides a particle sorting kit in which the fitting portion is provided with a protrusion that is convex in the direction of the tube. In the particle sorting kit relating to this technology, the protrusions can be side-side protrusions that are provided protruding in the lateral direction of the tube. In the particle sorting kit according to this technology, the protrusion can be a connection surface side protrusion that is provided in the direction of the connection end of the tube with the filter portion. Furthermore, in the particle sorting kit relating to this technology, the aforementioned protrusions are: The tube has a side projection that protrudes in the lateral direction, A connection surface side protrusion is provided on the tube in the direction of the connection end with the filter portion, It can also be equipped with this feature. In the particle sorting kit according to this technology, the side protrusion can be configured to be continuous in the longitudinal direction of the tube and to be located in the direction of the connection end of the tube to the filter portion, rather than the adhesive position that bonds the tube to the fitting portion. In the particle sorting kit relating to this technology, the side protrusion may be continuous in the longitudinal direction of the tube and may have a gradient that narrows the inner diameter of the fitting portion toward the filter direction. In this case, the gradient is A first gradient connected to the inner surface of the fitting portion, A second slope is connected to the first slope and is connected to the connection surface with the tube at the fitting portion, It can be equipped with. Furthermore, the connecting section between the first gradient and the second gradient can be given a rounded edge (R-shape). In the particle sorting kit according to this technology, a single fitting portion can be provided with multiple side protrusions. In the particle sorting kit relating to this technology, the connection surface of the fitting portion with the tube may be provided with an opening that communicates with the filter, and the protrusion on the connection surface side may be provided around the entire circumference of the opening. In the particle sorting kit relating to this technology, the contact portion of the protrusion on the connecting surface side with the tube can be rounded (R-shaped).

[0011] In this technology, "particles" can broadly include bio-related microparticles such as cells, microorganisms, and ribosomes, or synthetic particles such as latex particles, gel particles, and industrial particles.

[0012] Bio-related microparticles include chromosomes, ribosomes, mitochondria, and organelles (cellular organelles) that make up various cells. Cells include animal cells (e.g., hematopoietic cells) and plant cells. Microorganisms include bacteria such as E. coli, viruses such as tobacco mosaic virus, and fungi such as yeast. Furthermore, bio-related microparticles also include bio-related macromolecules such as nucleic acids, proteins, and complexes thereof. Industrial particles may be, for example, organic or inorganic polymer materials, metals, etc. Organic polymer materials include polystyrene, styrene-divinylbenzene, polymethyl methacrylate, etc. Inorganic polymer materials include glass, silica, magnetic materials, etc. Metals include gold colloid, aluminum, etc. The shape of these microparticles is generally spherical, but in this technology they may be non-spherical, and their size, mass, etc., are not particularly limited. [Brief explanation of the drawing]

[0013] [Figure 1] This is a schematic conceptual diagram showing a first embodiment of the particle sorting kit 1 related to this technology. [Figure 2] This is an enlarged conceptual diagram schematically showing an example of an embodiment of the microchip T that can be used in the particle sorting kit 1 related to this technology. [Figure 3] It is an enlarged perspective sectional view schematically showing an example of an embodiment of a filter unit 14 used in the particle separation kit 1 according to the present technology. [Figure 4] It is an enlarged sectional view schematically showing an example of a method of laminating the filter 141 used in the present technology. [Figure 5] It is a conceptual diagram schematically showing a method of connecting the filter unit 14 and the tube 15. A shows the state of the filter unit 14 and the tube 15 before connection, and B is a conceptual diagram showing the state where the filter unit 14 and the tube 15 are connected. [Figure 6] It is an enlarged sectional view schematically showing a first embodiment of the filter unit 14 used in the particle separation kit 1 according to the present technology. [Figure 7] A is an enlarged sectional view further enlarging the fitting portion 142 (the dashed line portion in FIG. 6) of the filter unit 14 according to the first embodiment in FIG. 6. B is an enlarged sectional view further enlarging the dashed circle portion of A. [Figure 8] It is an enlarged schematic view showing two examples of the fitting portion 142 of the filter unit 14 viewed from the insertion direction of the tube 15. [Figure 9] It is an enlarged sectional view schematically showing a second embodiment of the filter unit 14 used in the particle separation kit 1 according to the present technology. [Figure 10] It is an enlarged sectional view further enlarging the fitting portion 142 (the dashed line portion in FIG. 9) of the filter unit 14 according to the second embodiment in FIG. 9. [Figure 11] It is an enlarged sectional view schematically showing a third embodiment of the filter unit 14 used in the particle separation kit 1 according to the present technology. [Figure 12] A is an enlarged sectional view further enlarging the fitting portion 142 (the dashed line portion in FIG. 11) of the filter unit 14 according to the third embodiment in FIG. 11. B is an enlarged sectional view further enlarging the dashed circle portion of A. [Figure 13] It is a conceptual diagram schematically showing a second embodiment of the particle separation kit 1 according to the present technology. [Figure 14] It is a conceptual diagram schematically showing a third embodiment of the particle separation kit 1 according to the present technology. [Figure 15]This is a schematic conceptual diagram illustrating an example of an embodiment of the particle sorting apparatus 2 related to this technology. [Figure 16] This is a schematic conceptual diagram illustrating an example of an embodiment of the particle sorting system 3 related to this technology. [Figure 17] This is a schematic conceptual diagram illustrating a different example from Figure 10 of the embodiment of the particle sorting system 3 related to this technology. [Figure 18] This is a conceptual diagram showing a typical external diameter fitting structure. [Figure 19] This is a conceptual diagram showing a typical external diameter fitting structure. [Figure 20] This is a cross-sectional view of a filter structure that has been commonly used in the past. [Modes for carrying out the invention]

[0014] The following describes a suitable embodiment for implementing this technology with reference to the drawings. The embodiments described below are representative examples of the present technology and should not be interpreted as narrowing the scope of the present technology. The explanation will be given in the following order. 1. Particle sorting kit 1 (1) Sample storage section 11 (2) Sample channel 12 (3) Detection area 13 (4) Filter section 14 (4-1) Filter 141 (4-2) Fitting part 142 (4-3) Tapered section 143 (5) Tube pump section 16 (6) Target particle storage section 17 (7) Disposal section 18 (8) Sheath fluid storage section 19 (9) Gate liquid storage section 20 2. Particle sorting device 2, Particle sorting system 3 (1) Particle separation kit 1 (2) Light irradiation section 21 (3) Light detection unit 22 (4) Information Processing Unit 23 (5) Storage section 24 (6)Display section 25 (7) User Interface 26

[0015] 1. Particle sorting kit 1 Figure 1 is a schematic conceptual diagram showing a first embodiment of the particle sorting kit 1 according to this technology. The particle sorting kit 1 according to this technology comprises at least a sample storage section 11, a sample flow path 12, a detection area 13, and a filter section 14. It can also optionally include a tube pump section 16, a target particle storage section 17, a waste section 18, a sheath liquid storage section 19, a gate liquid storage section 20, etc. The particle sorting kit 1 will be described in detail below.

[0016] (1) Sample storage section 11 The sample storage section 11 contains a sample liquid containing particles to be separated. The sample storage section 11 can be formed, for example, from a cylindrical body with one end open and a lid that fits onto the cylindrical body and closes the opening. The lid has multiple opening valves for containing the sample liquid into the cylindrical body, and each opening valve employs a check valve configuration. Therefore, when the sample liquid is contained in the sample storage section 11 via the opening valves, the sample liquid cannot escape to the outside of the sample storage section 11. Furthermore, the configuration of the opening valves seals the sample liquid from the outside atmosphere.

[0017] The sample solution is not particularly limited and is not limited to any sample containing target particles separated using the particle separation kit 1 related to this technology. Specifically, examples include whole blood, peripheral blood mononuclear cells contained in whole blood, cell suspensions containing only lymphocytes, and other liquids containing patient-derived cells.

[0018] The sample containment section 11 may be equipped with a substance that suppresses the aggregation of particles in the sample solution. By using a substance that suppresses the aggregation of particles in the sample solution, the aggregation of particles in the sample solution can be suppressed, and any aggregates that still occur can be removed by the filter section 14 described later, thus more reliably removing impurities from the sample solution.

[0019] Substances that suppress particle aggregation include deoxyribonuclease (DNase), ethylenediaminetetraacetic acid (EDTA), and poloxamer (for example, BASF's "Pluronic F68").

[0020] When a substance that suppresses particle aggregation is included, the solution used for the sample solution can be a commonly used solution as long as it does not impair the effectiveness of this technology; however, in this technology, it is preferable to use phosphate-buffered saline (PBS).

[0021] When using PBS, it is preferable to use one that does not contain calcium and magnesium ions. However, when using the aforementioned substance that suppresses particle aggregation, it is also possible to use one that contains calcium and magnesium ions, depending on the type of substance used. Specifically, in this technology, PBS (without Ca 2+ Mg 2+ ), PBS (with Ca 2+ Mg 2+ ) + DNase, PBS (without Ca 2+ Mg 2+ It is preferable to use it in combination with EDTA, and it is also possible to add poloxamer to these combinations. In addition, albumin such as bovine serum albumin or human serum albumin can be added at a concentration of, for example, about 0.5%.

[0022] Furthermore, in the particle sorting kit 1 relating to this technology, a pre-sample storage section 111 is provided upstream of the sample storage section 11, and the pre-sample storage section 111 may contain a substance that suppresses the aggregation of particles in the sample liquid or other agents.

[0023] (2) Sample channel 12 Figure 2 is a schematic, enlarged conceptual diagram illustrating an example of an embodiment of a microchip T equipped with a sample channel 12 that can be used in the particle sorting kit 1 related to this technology. The sample channel 12 that can be used in this technology can be provided in a microchip T as shown in Figure 2, for example, but is not limited to this, and a channel such as those used in conventional flow cytometers can also be used, although not shown.

[0024] The sample liquid containing particles is introduced into the sample channel 12 from the sample inlet 121. The sheath liquid introduced from the sheath inlet 411 is divided and delivered into two sheath channels 41a and 41b. The sample channel 12 and the sheath channels 41a and 41b merge to form the main channel 124. The sample liquid layer flow delivered through the sample channel 12 and the sheath liquid layer flow delivered through the sheath channels 41a and 41b merge within the main channel 124, forming a sheath flow in which the sample liquid layer flow is sandwiched between the sheath liquid layer flows.

[0025] (3) Detection area 13 In Figure 2, reference numeral 13 indicates a detection region where excitation light is irradiated by a light irradiation unit 21 (described later), and fluorescence and scattered light are detected by a photodetection unit 22 (described later). The particles are sent to the detection region 13 in a single line arrangement in the sheath flow formed in the main channel 124, and are irradiated by excitation light from the light irradiation unit 21.

[0026] The main channel 124 branches into three channels downstream of the detection region 13. The main channel 124 communicates with three branch channels downstream of the detection region 13: the preparative channel 51 and the waste channels 52a and 52b. Of these, the preparative channel 51 is the channel into which particles determined to satisfy predetermined optical characteristics (also referred to as "target particles") are taken in. On the other hand, particles determined not to satisfy predetermined optical characteristics (also referred to as "non-target particles") are not taken into the preparative channel 51 but flow into either of the two waste channels 52a or 52b.

[0027] The target particles are taken into the preparative channel 51 by generating negative pressure within the preparative channel 51 using a piezoelectric element such as a piezo element, and this negative pressure is used to draw the sample containing the target particles and the sheath liquid into the preparative channel 51. The piezoelectric element is positioned in contact with the surface of the microchip T and is located at a position corresponding to the preparative channel 51. More specifically, the piezoelectric element is positioned at a position corresponding to the pressure chamber 511, which is provided as an expanded region within the preparative channel 51.

[0028] As shown in Figure 2, the interior of the pressure chamber 511 is expanded in the planar direction (width direction of the preparative channel 51) and also in the cross-sectional direction (height direction of the preparative channel 51). In other words, the preparative channel 51 is expanded in both the width and height directions within the pressure chamber 511. To put it another way, the preparative channel 51 is formed in the pressure chamber 511 such that the cross-section perpendicular to the flow direction of the sample and sheath fluid is large.

[0029] The piezoelectric element generates expansion and contraction forces in response to changes in the applied voltage, causing a pressure change within the preparative channel 51 via the surface (contact surface) of the microchip T. As flow occurs within the preparative channel 51 due to the pressure change, the volume within the preparative channel 51 changes simultaneously. The volume within the preparative channel 51 changes until it reaches a volume determined by the displacement of the piezoelectric element corresponding to the applied voltage. More specifically, when the piezoelectric element is expanded by the applied voltage, it presses against the displacement plate constituting the pressure chamber 511, keeping the volume of the pressure chamber 511 small. Then, when the applied voltage decreases, the piezoelectric element generates a force in the direction of contraction, reducing the pressure on the displacement plate and generating negative pressure within the pressure chamber 511.

[0030] In this technology, in order to efficiently transmit the expansion and contraction force of the piezoelectric element into the pressure chamber 511, it is preferable to create a depression on the surface of the microchip T at a position corresponding to the pressure chamber 511 and to place the piezoelectric element in the depression. This makes the displacement plate, which is the contact surface of the piezoelectric element, thinner, and allows the displacement plate to be easily displaced by the change in pressing force due to the expansion and contraction of the piezoelectric element, thereby causing a change in the volume of the pressure chamber 511.

[0031] The microchip T can be formed by bonding substrate layers on which sample channels 12 and preparative channels 51 are formed. The sample channels 12 and preparative channels 51 on the substrate layers can be formed by injection molding of a thermoplastic resin using a mold. Conventional plastics known as microchip materials, such as polycarbonate, polymethyl methacrylate (PMMA), cyclic polyolefin, polyethylene, polystyrene, polypropylene, and polydimethylsiloxane (PDMS), can be used as the thermoplastic resin. The number of substrate layers constituting the microchip T is not particularly limited and can consist of, for example, two or more layers.

[0032] The microchip T used in this technology may further include a gate liquid inlet 611 through which the gate liquid is introduced, and a gate channel 61 through which the gate liquid introduced from the gate liquid inlet 611 flows. The gate channel 61 is connected to, for example, one or more of the three branch channels of the preparative channel 51 and the waste channels 52a and 52b, and extends to the preparative channel 51 up to the pressure chamber 511, or is provided so as to intersect them perpendicularly, for example. The "gate liquid" is the liquid that flows through the gate channel 61 and serves as the main solvent for the sample, such as fine particles, recovered after preparative processing. Therefore, various liquids can be selected depending on the application. For example, a liquid medium used for liquids containing particles, a sheath liquid, or, in the case of protein particles, a buffer solution with a surfactant and adjusted pH, etc., can be used.

[0033] In particular, when the particles are cells, cell culture medium, cell preservation medium, etc., can be used as a gate solution. When using cell culture medium, it is suitable for subsequent processes applied to the separated and recovered cells, such as cell culture, cell activation, and gene transfer. When using cell preservation medium, it is suitable for storing and transporting the recovered cells. Furthermore, if the separated and recovered cells are undifferentiated cells such as iPS cells, a differentiation induction medium can be used, allowing for efficient progress to the next step.

[0034] Similarly, a variety of liquids can be selected for the sheath fluid. In this specification, the flow formed by the gate fluid is referred to as the "gate flow".

[0035] The upstream side of the gate channel 61 can be independently introduced from the gate flow inlet 611 and flowed at an appropriate flow rate. In this technology, the flow rate of the liquid introduced into the gate channel 61 is less than the flow rate of the liquid introduced into the sheath channels 41a and 41b, making it economical when expensive liquids such as cell culture medium, cell preservation medium, and differentiation induction medium are used only in the gate channel 61.

[0036] Furthermore, the gate flow can also be generated by branching off from the sheath liquid flow. For example, the sheath flow channels 41a and 41b after the sheath liquid inlet can be connected to the upstream end of the gate flow channel 61, so that the sheath liquid flow branches off and flows into the gate flow channel 61, thereby creating a gate flow. In this case, it is necessary to appropriately design the flow resistance of the gate flow channel 61 so that the gate flow rate is an appropriate flow rate.

[0037] Where the gate channel 61 and the preparative channel 51 intersect, a gate flow is generated that flows straight through the gate channel 61, as well as gate flows directed toward the detection area 13 and the pressure chamber 511. The latter gate flow prevents particles that should not be acquired (non-target particles) from entering the pressure chamber 511 side of the preparative channel 51. The gate flow that has flowed through the gate channel 61 flows out into the preparative channel 51, where it branches into gate flows directed toward the detection area 13 and the pressure chamber 511 side of the preparative channel 51. The former gate flow prevents non-target particles from entering the pressure chamber 511 side of the preparative channel 51.

[0038] The microchip T that can be used in this technology is connected to a sample containment section 11, a filter section 14 (described later), etc., and may be distributed as part of an item such as a cartridge, unit, device, kit, or instrument for a closed-type cell sorter.

[0039] (4) Filter section 14 Figure 3 is a schematic enlarged perspective cross-sectional view showing an example of an embodiment of the filter section 14 used in the particle sorting kit 1 according to this technology. The filter section 14 comprises at least a filter 141 and a fitting section 142. A tapered section 143 may also be included as needed.

[0040] (4-1) Filter 141 The filter 141 that can be used in the filter section 14 of the particle sorting kit 1 related to this technology can be designed in terms of its material, inner diameter, pore size, etc., according to the size and shape of the target particles to be sorted.

[0041] The material used for the filter 141 in this technology can be nylon, polyethylene terephthalate (PET), polyethylene (PE), etc. Among these, in this technology, it is preferable to manufacture the filter using nylon from the viewpoint of pore size, sterilization properties, and adhesive properties.

[0042] Furthermore, the inner diameter of the filter 141 used in this technology is preferably 0.5 to 10 mm, and more preferably 2 to 7 mm. The smaller the area of ​​the filter 141, the smaller the loss of target particles can be.

[0043] Furthermore, the pore size of the filter 141 used in this technology can be designed according to the type of target particle. For example, if the target particle is a blood cell, it is preferable to have a pore size of 20 to 100 μm. If the target particle is an iPS cell or the like, it can be 100 μm or larger. In this way, by designing the pore size of the filter 141 according to the type of target particle, impurities can be efficiently removed while preventing damage or death of the target particle.

[0044] Furthermore, multiple filters 141 can be used in a stacked configuration. By stacking two or more filters 141, it is possible to more reliably prevent the outflow of particle aggregates and foreign matter. When stacking multiple filters 141, they can be stacked directly, but as shown in Figure 4, filters 141a and 141b can also be stacked with O-rings in between.

[0045] (4-2) Fitting part 142 The filter section 14 of the particle sorting kit 1 according to this technology is equipped with a fitting section 142 for outer diameter fitting of a tube 15 for connection to the sample storage section 11 and / or the sample flow path 12. Since the tube 15 used in this technology has a very narrow inner diameter, it is not possible to connect it using a barb shape like a typical tube. Therefore, by providing the filter section 14 used in this technology with a fitting section 142 that presses the tube 15 from the outside and fits it to the outside, it becomes possible to connect it to a tube 15 with a narrow inner diameter.

[0046] Figure 5 is a conceptual diagram showing how to connect the filter unit 14 and the tube 15. Figure 5A shows the filter unit 14 and the tube 15 before connection, and Figure 5B is a conceptual diagram showing the state in which the filter unit 14 and the tube 15 are connected. As shown in Figure 5B, the filter unit 14 and the tube 15 can be connected by pushing the tube 15 into the fitting portion 142 of the filter unit 14.

[0047] Figures 18 and 19 are conceptual diagrams showing a typical outer diameter fitting structure. When mass-producing tubes and outer diameter fitting structures, both have dimensional tolerances. If the internal dimensions of the outer diameter fitting structure are too small or the tube is too large, problems arise such as the tube not being able to be inserted all the way in (see Figure 18A). Conversely, if the internal dimensions of the outer diameter fitting structure are too large or the tube is too small, problems arise such as the fitting being too loose and the tube not being held in place (see Figure 18B). These issues also resulted in dead volume.

[0048] Furthermore, while tube ends are cut with cutters, scissors, or specialized tools, achieving a perfectly flat cut requires high precision and is considered impractical for mass production. As shown in Figure 19, if the tube is cut at an angle, when the tube is inserted into the filter port, it may appear to be inserted all the way in, but in reality, one side will be floating. This resulting gap also causes dead volume.

[0049] On the other hand, in this technology, by providing a protrusion 1421 that protrudes in the direction of the tube 15 on the fitting portion 142 of the filter portion 14, the connectivity between the tube 15 and the filter portion 14 can be improved and the dead volume can be reduced. The specific structure of the protrusion 1421 will be described in detail below.

[0050] [First embodiment of filter unit 14] Figure 6 is a schematic enlarged cross-sectional view showing a first embodiment of the filter section 14 used in the particle sorting kit 1 according to this technology. Figure 7A is a further enlarged cross-sectional view of the fitting section 142 portion (dashed line portion in Figure 6) of the filter section 14 according to the first embodiment. Figure 7B is a further enlarged cross-sectional view of the dashed circle portion in Figure 7A. The fitting section 142 of the filter section 14 according to the first embodiment is provided with a side projection 1421a that protrudes in the lateral direction of the tube 15.

[0051] As mentioned above, in a typical outer diameter fitting structure, the side surface of the tube is supported by the entire inner surface that fits. Therefore, if the internal dimensions of the outer diameter fitting structure are too small or the tube is too large, problems arise such as the tube not being able to be inserted all the way in (Figure 18A). However, in this technology, when the tube 15 is inserted into the fitting portion 142, the side projection 1421a first comes into contact with the tube 15. If the outer diameter of the tube is larger than the internal dimensions of the side projection 1421a, the tube 15 is compressed at the side projection 1421a, allowing the tube 15 to be inserted all the way into the fitting portion 142.

[0052] Furthermore, the side projection 1421a allows the tube 15 to be easily crushed, making it possible to set the outer diameter of the tube 15 to be larger than the inner diameter of the side projection 1421a portion of the fitting portion 142. This prevents the tube 15 from becoming loose when inserted, as shown in Figure 18B, improving the retention of the tube 15, as well as improving workability and enabling positioning such as centering of the tube 5.

[0053] The shape of the side protrusion 1421a can be freely designed as long as it does not impair the effectiveness of this technology. In this technology, it is preferable that the side protrusion 1421a be continuous in the longitudinal direction (sample liquid flow direction F) of the tube 15. In this technology, since the tube 15 is supported by the side protrusion 1421a, making it continuous in the longitudinal direction of the tube 15 prevents the tube 15 from wobbling, contributing to improved holding ability of the tube 15 and improved workability.

[0054] The lower limit of the longitudinal length L1 of the side projection 1421a can also be freely designed as long as it does not impair the effects of this technology, but in this technology, it is preferably 0.5 mm or more, and more preferably 1.0 mm or more. Setting it to 0.5 mm or more can further improve the holding ability and workability of the tube 15. Furthermore, in the case of the third embodiment described later (see Figure 12), it is preferable to set the lower limit of the longitudinal length L1 of the side projection 1421a to be greater than or equal to the height H2 of the connecting surface projection 1421b (see Figure 10), and more preferably to be greater than or equal to the height H2 + 0.5 mm of the connecting surface projection 1421b.

[0055] Furthermore, it is preferable that the side projection 1421a is located in the direction of the connection end 151 of the tube 15 with the filter portion 14, rather than the adhesive position that bonds the tube 15 and the fitting portion 142. This is because, generally, with the tube 15 inserted into the fitting portion 142, adhesive is often poured into the gap I between the tube 15 and the fitting portion 142, indicated by reference numeral I in Figure 7, to fix it in place. Therefore, it is preferable that the side projection 1421a is continuous from the connection surface 1422 of the fitting portion 142 with the tube 15 down to below the adhesive position.

[0056] The upper limit of the longitudinal length L1 of the side projection 1421a can also be freely designed as long as it does not impair the effectiveness of this technology. However, in this technology, it is preferable that the length is less than or equal to the height of the filter port used, and more preferably less than or equal to the height of the filter port minus 1 mm. By setting the length to be less than or equal to the height of the filter port, a sufficient amount of adhesive necessary for bonding can be poured into the gap I between the tube 15 and the fitting portion 142, and leakage of the adhesive can also be prevented.

[0057] The height H1 of the side projection 1421a from the inner surface 1423 of the fitting portion 142 can also be freely designed as long as it does not impair the effects of this technology. In the first embodiment, it is preferable to design the height H1 so that the tip of the tube 15 can reach the connection surface 1422 with the tube 15 in the fitting portion 142. In the third embodiment described later (see Figure 12), it is preferable to design the height H1 so that the tip of the tube 15 can reach the connection surface side projection 1421b. By designing in this way, the holding ability of the tube 15, the ease of assembly, and the positioning performance of the tube 15 can be improved.

[0058] Preferably, the lateral protrusions 1421a of the tube 15, which are continuous in the longitudinal direction, have a gradient such that the inner diameter of the fitting portion 142 narrows toward the filter 141. This configuration facilitates the insertion of the tube 15, improves assembly workability, and also facilitates the removal of the mold during manufacturing.

[0059] In this case, it is preferable that the gradient comprises a first gradient 1423S connected to the inner surface 1423 of the fitting portion 142, and a second gradient 1422S connected to the first gradient 1423S and connected to the connection surface 1422 with the tube 15 in the fitting portion 142. Having two stages of gradient in this way makes it easier to insert the tube 15, further improving assembly workability, and also makes it easier to remove the mold during manufacturing.

[0060] The connecting section between the first gradient 1423S and the second gradient 1422S is preferably rounded (R-shaped). Rounding makes it easier to insert the tube 15 and further improves assembly workability.

[0061] The angle α1 of the first gradient 1423S with respect to the inner surface 1423 of the fitting portion 142 can be freely designed as long as the effects of this technology are not impaired. However, in this technology, an angle α2 or more and 60° or less with respect to the inner surface 1423 of the fitting portion 142, as described later, is preferred, and an angle α2 or more and 45° or less is more preferred.

[0062] The angle α2 of the second gradient 1422S with respect to the inner surface 1423 of the fitting portion 142 can be freely designed as long as the effectiveness of this technology is not impaired, but in this technology, 0.1 to 10° is preferred, and 0.1 to 5° is more preferred.

[0063] The number of side protrusions 1421a described above is not particularly limited as long as it does not impair the effectiveness of this technology, but it is preferable to have multiple protrusions on one fitting portion 142. Figure 8 is an enlarged schematic diagram showing two examples of the fitting portion 142 of the filter portion 14 viewed from the direction in which the tube 15 is inserted. The example shown in Figure 8A is an example in which three side protrusions 1421a are provided on one fitting portion 142, and the example shown in Figure 8B is an example in which six side protrusions 1421a are provided on one fitting portion 142.

[0064] By providing multiple side projections 1421a on a single fitting portion 142, the holding ability of the tube 15, workability, and positioning performance of the tube 5 can be improved. There is no particular upper limit to the number of side projections 1421a, but if there are too many, it may become difficult to insert the tube 15 all the way in, or it may lead to increased manufacturing complexity and costs, or a decrease in positioning accuracy such as centering the tube. Therefore, it is preferable that the number of side projections 1421a on a single fitting portion 142 be 6 or less, and more preferably 3 or less.

[0065] [Second embodiment of filter unit 14] Figure 9 is a schematic enlarged cross-sectional view showing a second embodiment of the filter section 14 used in the particle sorting kit 1 according to this technology. Figure 10 is a further enlarged cross-sectional view of the fitting section 142 portion (dashed line portion in Figure 9) of the filter section 14 according to the second embodiment. The fitting section 142 of the filter section 14 according to the second embodiment is provided with a connection surface side protrusion 1421b as a protrusion, which is provided protruding in the direction of the connection end of the tube 15 with the filter section 14.

[0066] As mentioned above, in a typical outer diameter fitting structure, the connecting surface of the tube is supported by the entire connecting surface (bottom surface) of the fitting structure. Therefore, if the tube is cut at an angle, a part of the connecting end of the tube will float up (see Figure 19). However, in this technology, when the tube 15 is inserted into the fitting part 142, the connecting end 151 of the tube 15 is crushed by the protrusion 1421b on the connecting surface side, allowing the tube 15 to be inserted all the way into the fitting part 142. This prevents a part of the connecting end of the tube from floating up as shown in Figure 19, reduces unnecessary space other than the flow path for the sample liquid, and as a result, reduces dead volume.

[0067] The connection surface 1422 of the fitting portion 142 with the tube 15 is provided with an opening O that leads to the filter 141. Preferably, the inner diameter of the opening O is wider than the inner diameter of the flow path of the tube 15. By making the inner diameter of the opening O wider than the inner diameter of the flow path of the tube 15, it is possible to prevent leakage of the sample flow and blockage of the flow path hole.

[0068] Furthermore, it is preferable to provide the connection surface side protrusion 1421b around the entire circumference of the opening O. That is, it is preferable to form the outer circumference of the opening O with the connection surface side protrusion 1421b (see Figure 8). By providing the connection surface side protrusion 1421b around the entire circumference of the opening O, it becomes possible to more reliably prevent leakage of the sample flow.

[0069] Furthermore, it is preferable that the contact portion of the connection surface-side protrusion 1421b with the tube 15 be rounded. This allows the connection surface 151 of the tube 15 and the connection surface-side protrusion 1421b to contact each other along a line rather than a surface, making the tube 15 more easily compressible and allowing it to be inserted all the way into the fitting portion 142. As a result, the dead volume can be reduced even more reliably, and the area from the flow path of the tube 15 to the opening O is sealed, further reliably preventing leakage of the sample flow.

[0070] The height H2 of the protrusion 1421b on the connecting surface side from the connecting surface 1422 can be freely designed as long as it does not impair the effects of this technology, but in this technology, 0.2 to 5 mm is preferred, and 0.4 to 2 mm is more preferred. By setting the height H2 of the protrusion 1421b on the connecting surface side from the connecting surface 1422 to 0.2 to 5 mm, the holding ability of the tube 15, the ease of assembly, and the positioning performance of the tube 15 can be improved. In addition, by setting the height H2 to 0.2 mm or more, leakage of the sample liquid can be prevented, and by setting the height H2 to 5 mm or less, it is possible to prevent the adhesive from flowing into the connecting surface 1422 side.

[0071] The width d1 of the protrusion 1421b on the connecting surface can be freely designed as long as it does not impair the effectiveness of this technology, but in this technology, 0.2 to 5 mm is preferred, and 0.4 to 2 mm is more preferred. By setting the width d1 of the protrusion 1421b on the connecting surface to 0.2 to 5 mm, the holding ability of the tube 15, the ease of assembly, and the positioning performance of the tube 15 can be improved. In addition, by setting the width d1 to 0.2 mm or more, leakage of the sample liquid can be prevented.

[0072] [Third embodiment of filter unit 14] Figure 11 is a schematic enlarged cross-sectional view showing a third embodiment of the filter section 14 used in the particle sorting kit 1 according to this technology. Figure 12 is a further enlarged cross-sectional view of the fitting section 142 portion (dashed line portion in Figure 11) of the filter section 14 according to the third embodiment. The fitting section 142 of the filter section 14 according to the third embodiment includes a side-side protrusion 1421a that protrudes in the lateral direction of the tube 15, and a connection-side protrusion 1421b that protrudes in the direction of the connection end of the tube 15 with the filter section 14. That is, the third embodiment is an example in which the protrusion 1421 includes both the side-side protrusion 1421a of the first embodiment and the connection-side protrusion 1421b of the second embodiment. The details of the side-side protrusion 1421a and the connection-side protrusion 1421b are the same as those of the first and second embodiments described above, so they will not be explained here.

[0073] The fitting portion 142 described above is present both upstream and downstream of the filter 141, but the form of the upstream fitting portion 142 and the downstream fitting portion 142 may be the same or different. For example, the form of the fitting portion 142 upstream of the filter 141 may be the first embodiment, and the form of the fitting portion 142 downstream of the filter 141 may be the third embodiment. Also, for example, even if the form of the fitting portion 142 upstream of the filter 141 and the downstream fitting portion 142 are the same embodiment, the fine dimensions may be designed to be different depending on the form of the tube 15 used and the form of the flow path of the tube 15.

[0074] The fitting portion 142 preferably has a tapered structure that narrows in diameter toward the filter 141. The taper angle β1 of the tapered structure shown in Figure 6 can be designed according to the shape of the tube 15 to be connected, but in this technology, it is preferable to set the taper angle β1 of the tapered structure to 80 to 90°. By designing the taper angle β1 of the tapered structure within this range, reliable connection can be ensured even if there is some variation in the outer diameter of the tube 15 or slight variation in the dimensions of the fitting portion 142 during manufacturing.

[0075] Furthermore, the inner diameter d2 and longitudinal length L2 of the fitting portion 142 on the filter 141 side, as shown in Figure 5, can be designed according to the shape of the tube 15 used. For example, when using a tube 15 with an outer diameter d3 of 3.4 to 3.5 mm, it is preferable to design the inner diameter d2 of the fitting portion 142 on the filter 141 side to be 3.3 to 3.6 mm and the longitudinal length L2 of the fitting portion 142 to be 15 to 25 mm. By designing the inner diameter d2 of the fitting portion 142 on the filter 141 side and the longitudinal length L2 of the fitting portion 142 according to the shape of the tube 15 used, a reliable connection can be ensured even if there is some variation in the dimensions of the fitting portion 142 during manufacturing.

[0076] (4-3) Tapered section 143 The filter section 14 of the particle sorting kit 1 according to this technology may include a tapered section 143 downstream of the filter 141. This tapered section 143 can be configured to narrow the flow path diameter along the flow direction F of the sample liquid.

[0077] Figure 20 is a cross-sectional view of a conventionally used filter structure. In conventional filter structures, particles in the sample liquid that pass through filter 1411 settle in the area indicated by the dashed line in Figure 20, resulting in a problem of particle loss.

[0078] On the other hand, by providing a tapered section 143 downstream of the filter 141, it is possible to prevent particles in the sample liquid that have passed through the filter 141 from settling on the inner wall surface of the filter section 14, thereby reducing the amount of particle loss.

[0079] In the particle sorting kit 1 relating to this technology, the taper angle β2 of the tapered portion 143 shown in Figure 6 is preferably 50 to 80°. By setting the taper angle β2 of the tapered portion 143 to 50° or more, it is possible to further advantageously prevent particles in the sample liquid that have passed through the filter 141 from settling on the inner wall surface of the filter portion 14. Furthermore, by setting the taper angle β2 of the tapered portion 143 to 80° or less, it is possible to miniaturize the filter portion 14.

[0080] The filter section 14 described above can be placed in any position as long as it does not impair the effectiveness of this technology. For example, by placing it upstream of the sample storage section 11, as in the first embodiment of the particle sorting kit 1 related to this technology shown in Figure 1, it is possible to prevent foreign matter from entering the sample storage section 11 at an initial stage.

[0081] Furthermore, as shown in the second embodiment of the particle sorting kit 1 according to this technology in Figure 13, for example, a filter unit 14 can be placed between the sample containment unit 11 and the microchip T (detection region 13). In this case, it is preferable to place the filter unit 14 directly in front of the microchip T. By placing the filter unit 14 directly in front of the microchip T (detection region 13), it is possible to reliably prevent foreign matter from entering the microchip T (detection region 13), and as a result, the accuracy of analysis and target particle sorting performed within the microchip T can be improved.

[0082] Furthermore, as shown in the third embodiment of the particle sorting kit 1 according to this technology in Figure 14, for example, filter units 14a and 14b can also be placed in two locations: upstream of the sample storage unit 11 and between the sample storage unit 11 and the microchip T (detection region 13). With this arrangement, the filter unit 14a, placed upstream of the sample storage unit 11, prevents foreign matter from entering the sample storage unit 11 at an initial stage, while the filter unit 14b, placed between the sample storage unit 11 and the microchip T (detection region 13), can remove aggregates formed as particles in the sample liquid flow from the sample storage unit 11 to the microchip T (detection region 13). As a result, the accuracy of analysis and target particle sorting performed within the microchip T can be improved.

[0083] (5) Tube pump section 16 The particle separation kit 1 according to this technology may be equipped with a tube pump unit 16. In the particle separation kit 1 according to this technology, the tube pump unit 16 may be made of an elastic material. Although a roller for squeezing the elastic tube may be provided in the particle separation kit 1 according to this technology, it is also possible to pass the sample liquid in the tube 15 by installing the tube pump unit 16 of the particle separation kit 1 according to this technology on the roller portion provided on the particle separation device 2 side, which will be described later.

[0084] In the particle sorting kit 1 according to this technology, the tube pump unit 16 can be positioned at any location as long as it does not impair the effects of the present invention. However, it is preferable to position it between the filter unit 14(14b) provided between the sample storage unit 11 and the microchip T, as shown in the second embodiment of the particle sorting kit 1 according to this technology shown in Figure 13, and the third embodiment of the particle sorting kit 1 according to this technology shown in Figure 14. Since particles in the sample liquid tend to aggregate in the tube pump unit 16, this arrangement allows the filter unit 14(14b) to remove aggregates formed while the sample is flowing through the tube pump unit 16, even if such aggregates are formed before reaching the microchip T. As a result, the accuracy of analysis and target particle sorting performed within the microchip T can be improved.

[0085] (6) Target particle storage section 17 The particle sorting kit 1 according to this technology may be equipped with a target particle storage section 17 as needed. The sorted target particles are contained in the target particle storage section 17. The target particle storage section 17 is formed, for example, in the shape of a bag that contains the target particles and is equipped with an opening valve connected to the sorting channel 51 of the microchip T. The opening valve employs a so-called check valve configuration, and when the target particles are contained in the target particle storage section 17 via the opening valve, the target particles are prevented from escaping to the outside of the target particle storage section 17. Furthermore, the configuration of the opening valve prevents the target particles from coming into contact with the external atmosphere.

[0086] The configuration of the target particle storage unit 17 described above is merely one example; any known configuration can be adopted as long as the target particles are not exposed to the external atmosphere.

[0087] (7) Disposal section 18 In the particle separation kit 1 according to this technology, when separating only the target particles from the sample liquid using the microchip T, it is necessary to remove non-target particles. Furthermore, since the microchip T forms a sheath flow to separate the target particles, it is necessary to remove the sample liquid containing non-target particles. For this reason, the particle separation kit 1 according to this technology may be equipped with a waste section 18 as needed. Particles other than the target particles are discarded in the waste section 18.

[0088] (8) Sheath fluid storage section 19 In the particle separation kit 1 according to this technology, a sheath flow is formed in the sample channel 12 to separate target particles from the sample liquid. Therefore, the particle separation kit 1 according to this technology may be equipped with a sheath liquid containment section 19 as needed. The sheath liquid containment section 19 contains sheath liquid.

[0089] The sheath fluid containment section 19 includes, for example, a tubular member into which the sheath fluid flows, and this tubular member is in communication with the sheath inlet 411 of the microchip T. As a result, the sheath fluid flows into the flow path of the microchip T, and a sheath flow is formed.

[0090] The configuration of the sheath fluid storage section 19 is not particularly limited, and a known configuration can be adopted. Furthermore, the configuration for discharging the sheath fluid from the sheath fluid storage section 19 is also not particularly limited; for example, a drive source such as an actuator may be used.

[0091] (9) Gate liquid storage section 20 Furthermore, the particle sorting kit 1 relating to this technology may be equipped with a gate liquid containment section 20 as needed. The gate liquid containment section 20 contains the gate liquid. The "gate liquid" is the same as described above, so its explanation is omitted here.

[0092] The gate liquid containment section 20 includes, for example, a tubular member into which the gate liquid flows, and this tubular member is in communication with the gate liquid inlet 611 of the microchip T. As a result, the gate liquid flows into the flow path of the microchip T, and target particles are separated.

[0093] The configuration of the gate fluid reservoir 20 is not particularly limited, and a known configuration can be adopted. Furthermore, the configuration for discharging the gate fluid from the gate fluid reservoir 20 is also not particularly limited; for example, a drive source such as an actuator may be used.

[0094] The parts of the particle sorting kit 1 described above can be sealed and connected in whole or in part. Therefore, the sorting and storage of target particles can be performed in a sealed space, thereby improving the accuracy of target particle sorting. Furthermore, contamination of the particle sorting kit itself by mist containing target particles and / or contamination of the sorted target particles with other substances can be prevented. As a result, the particle sorting kit 1 according to this technology can be applied to clinical applications such as immunotherapy where the purity of target particles is required.

[0095] Furthermore, the particle sorting kit 1 related to this technology can be made disposable, thereby avoiding risks such as contamination between samples and improving usability.

[0096] Furthermore, it is possible to equip the particle sorting kit 1 with multiple components. For example, although not shown in the diagram, by adding a microchip T downstream of the target particle storage unit 17, it is possible to further sort the target particles sorted from the sample liquid into finer particles.

[0097] 2. Particle sorting device 2, particle measurement system 3 Figure 15 is a schematic conceptual diagram illustrating an example of an embodiment of the particle sorting device 2 according to this technology. Figures 16 and 17 are schematic conceptual diagrams illustrating an example of an embodiment of the particle sorting system 3 according to this technology. Note that, due to space limitations, only the microchip T portion of the particle sorting kit 1 according to this technology is shown in Figures 15-17.

[0098] The particle sorting device 2 and particle sorting system 3 according to this technology comprise at least the particle sorting kit 1 described above, a light irradiation unit 21, and a light detection unit 22. Additionally, an information processing unit 23, a storage unit 24, a display unit 25, a user interface 26, etc., may be included as needed.

[0099] The information processing unit 23, storage unit 24, display unit 25, and user interface 26 may be provided within the particle sorting device 2, as shown in Figure 15, or the particle measurement system 3 may consist of an information processing device 4 equipped with the information processing unit 23, storage unit 24, display unit 25, and user interface 26, and the particle sorting device 2, as shown in Figure 16. Alternatively, as shown in Figure 17, the particle measurement system 3 can consist of an independent information processing unit 23, storage unit 24, display unit 25, and user interface 26 connected to the photodetector unit 22 of the particle sorting device 2 via a network.

[0100] Furthermore, the information processing unit 23, storage unit 24, and display unit 25 can be placed in a cloud environment and connected to the particle sorting device 2 via a network. In this case, the records of information processing in the information processing unit 23 can be stored in the storage unit 24, and the various types of information stored in the storage unit 24 can be shared among multiple users.

[0101] (1) Particle separation kit 1 The particle sorting device 2 is equipped with a particle sorting kit 1 for sorting and storing target particles. Since the particle sorting kit 1 is the same as described above, its explanation is omitted here.

[0102] (2) Light irradiation section 21 The light irradiation unit 21 irradiates light onto the sample to be separated. Specifically, the light irradiation unit 21 irradiates light (excitation light) onto particles passing through the detection region 13.

[0103] The light irradiation unit 21 is composed of, for example, a light source that emits excitation light and an objective lens that focuses the excitation light onto the sample liquid flowing through the main channel 124. The light source can be appropriately selected from laser diodes, SHG lasers, solid-state lasers, gas lasers, and high-brightness LEDs, depending on the purpose of the analysis. The light irradiation unit 21 may also have optical elements other than the light source and objective lens, if necessary.

[0104] (3) Light detection unit 22 The photodetector 22 detects fluorescence and scattered light emitted from the sample to be separated when irradiated with excitation light. Specifically, the photodetector 22 detects fluorescence and scattered light emitted from the sample and converts it into an electrical signal. This electrical signal is then output to the information processing unit 23, which will be described later.

[0105] The configuration of the light detection unit 22 is not particularly limited, and a known configuration can be adopted, and furthermore, the method of conversion to an electrical signal is not particularly limited.

[0106] (4) Information Processing Unit 23 The information processing unit 23 receives the electrical signal converted by the photodetector 22. Specifically, the information processing unit 23 determines the optical properties of the sample liquid and the target particles contained in the sample liquid based on the input electrical signal.

[0107] Furthermore, the information processing unit 23 includes a gating circuit for calculating thresholds for separating target particles from the sample solution, thresholds for determining whether or not a required number of target particles have been separated, and so on. When the threshold for separating target particles from the sample solution is calculated using the configuration of this gating circuit, it is converted into an electrical signal for separation, and this separation signal is output to a piezoelectric element on the microchip T.

[0108] Furthermore, the configuration of the information processing unit 23 is not particularly limited, and a known configuration can be adopted. In addition, the information processing method performed by the gating circuit of the information processing unit 23 can also be a known method.

[0109] (5) Storage section 24 The particle sorting device 2 and particle sorting system 3 related to this technology may be equipped with a storage unit 24 for storing various types of data. The storage unit 24 can store all matters related to measurement, such as optical information of particles detected by the photodetector 22 and records of information processing in the information processing unit 23.

[0110] Furthermore, as mentioned above, this technology allows the storage unit 24 to be located in a cloud environment, making it possible for each user to share various types of information recorded in the storage unit 24 on the cloud via the network.

[0111] In this technology, the memory unit 24 is not essential, and various types of data can be stored using external memory devices, etc.

[0112] (6)Display section 25 The particle sorting device 2 and particle sorting system 3 related to this technology may be equipped with a display unit 25 for displaying various information. The display unit 25 can display all matters related to the measurement, such as optical information of particles detected by the photodetector 22 and various data processed by the information processing unit 23.

[0113] In this technology, the display unit 25 is not essential, and an external display device may be connected. For example, a display or printer can be used as the display unit 25.

[0114] (7) User Interface 26 The particle sorting device 2 and particle sorting system 3 related to this technology may further be equipped with a user interface 26, which is a part for user operation. The user can access and control each part through the user interface 26.

[0115] In this technology, the user interface 26 is not mandatory, and an external operating device may be connected. For example, a mouse or keyboard can be used as the user interface 26.

[0116] Furthermore, this technology can also be configured as follows: (1) A sample container for containing a sample solution containing particles, A sample channel through which the aforementioned sample liquid flows, A detection region in which target particles are detected from the aforementioned sample liquid, A filter section comprising a filter and a fitting section for outer diameter fitting with a tube for connecting to the sample storage section and / or the sample flow path, Equipped with, The particle sorting kit is provided with a protrusion on the fitting portion that is convex in the direction of the tube. (2) The particle sorting kit according to (1), wherein the aforementioned protrusions include side protrusions that protrude in the lateral direction of the tube. (3) The particle sorting kit according to (1) or (2), wherein the aforementioned protrusion is a connection surface side protrusion that is provided in the direction of the connection end of the tube with the filter portion. (4) The aforementioned protrusion is, The tube has a side projection that protrudes in the lateral direction, A connection surface side protrusion is provided on the tube in the direction of the connection end with the filter portion, A particle sorting kit as described in (1), comprising: (5) The particle sorting kit according to (2) or (4), wherein the side projection is continuous in the longitudinal direction of the tube and is located in the direction of the connection end of the tube to the filter portion, rather than the adhesive position that bonds the tube to the fitting portion. (6) The particle sorting kit according to (2), (4), or (5), wherein the side projection is continuous in the longitudinal direction of the tube and has a gradient such that the inner diameter of the fitting portion narrows toward the filter direction. (7) The aforementioned gradient is, A first gradient connected to the inner surface of the fitting portion, A second slope is connected to the first slope and is connected to the connection surface with the tube at the fitting portion, A particle sorting kit as described in (6), comprising: (8) The connecting section between the first gradient and the second gradient is a particle sorting kit as described in (7), which is R-shaped. (9) A particle sorting kit according to any one of (2), (4), and (5) to (8), wherein a plurality of the side protrusions are provided on one of the fitting portions. (10) The connection surface of the fitting portion with the tube is provided with an opening that communicates with the filter. The connecting surface side protrusion is provided around the entire circumference of the opening, the particle sorting kit according to any one of (3) to (9). (11) The contact portion of the protrusion on the connecting surface side with the tube is R-shaped, as described in any of (3) to (9) of the particle separation kit. [Explanation of Symbols]

[0117] 1 Particle Separation Kit 11 Sample storage section 12 Sample Flow Channels 13 Detection area 14 Filter section 15 tubes 16 Tube pump section 17 Target Particle Storage Unit 18. Disposal Section 19. Sheath liquid storage section 20 Gate liquid containment section 2 Particle separation device 3. Particle sorting system 21 Light-irradiating section 22 Light detection unit 23 Information Processing Department 24 Memory section 25 Display section 26 User Interface

Claims

1. A sample container for containing a sample solution containing particles, A sample channel through which the aforementioned sample liquid flows, A detection region in which target particles are detected from the aforementioned sample liquid, A filter section comprising a filter and a fitting section for outer diameter fitting with a tube for connecting to the sample storage section and / or the sample flow path, Equipped with, The particle sorting kit is provided with a protrusion on the fitting portion that is convex in the direction of the tube.

2. The particle sorting kit according to claim 1, wherein the aforementioned protrusion is a side protrusion provided in the lateral direction of the tube.

3. The particle sorting kit according to claim 1, wherein the aforementioned protrusion is a connection surface side protrusion that protrudes in the direction of the connection end of the tube with the filter portion.

4. The aforementioned protrusion is, The tube has a side projection that protrudes in the lateral direction, A connection surface side protrusion is provided on the tube in the direction of the connection end with the filter portion, A particle separation kit according to claim 1, comprising:

5. The particle sorting kit according to claim 2, wherein the side projection is continuous with the longitudinal direction of the tube and is located in the direction of the connection end of the tube to the filter portion, rather than the adhesive position that bonds the tube to the fitting portion.

6. The particle sorting kit according to claim 2, wherein the side projection is continuous in the longitudinal direction of the tube and has a gradient such that the inner diameter of the fitting portion narrows toward the filter direction.

7. The aforementioned gradient is, A first gradient connected to the inner surface of the fitting portion, A second slope is connected to the first slope and is connected to the connection surface with the tube at the fitting portion, A particle separation kit according to claim 6, comprising the features described above.

8. The particle sorting kit according to claim 7, wherein the connecting portion between the first gradient and the second gradient is R-shaped.

9. The particle sorting kit according to claim 2, wherein a plurality of the side protrusions are provided on one of the fitting portions.

10. The connection surface of the fitting portion with the tube is provided with an opening that communicates with the filter. The particle sorting kit according to claim 3, wherein the convex portion on the connecting surface side is provided around the entire circumference of the opening.

11. The particle sorting kit according to claim 3, wherein the contact portion of the protrusion on the connecting surface side with the tube is rounded.

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