Method for preparing protein-oligonucleotide complexes

JP7863543B2Active Publication Date: 2026-05-21DYNE THERAPEUTICS INC
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
DYNE THERAPEUTICS INC
Filing Date
2021-09-03
Publication Date
2026-05-21

AI Technical Summary

Technical Problem

Existing methods face challenges in effectively delivering therapeutic oligonucleotides to target tissues or cells and in purifying protein-oligonucleotide complexes, particularly due to the difficulty in separating unconjugated oligonucleotides and proteins from the complexes.

Method used

A method involving the use of a mixed-mode resin with positively charged metal moieties and negatively charged ionic moieties, combined with organic solvents, to adsorb and elute protein-oligonucleotide complexes, effectively separating unconjugated components and producing high-purity complexes.

Benefits of technology

The method achieves high purity and yield of protein-oligonucleotide complexes, enhancing their delivery efficiency to target tissues or cells, particularly for treating muscle-specific diseases.

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Patent Text Reader

Abstract

Aspects of the present disclosure relate to methods for purifying complexes comprising a protein (e.g., an antibody) covalently linked to a molecular payload (e.g., a charge-neutral oligonucleotide, a charged oligonucleotide, or a hydrophobic small molecule) using a mixed-mode resin, e.g., a hydroxyapatite resin, containing positively charged metal sites and negatively charged ionic sites. Methods for producing the complexes are also provided.
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