Method for rearing artemias, feed for artemia, artemia and artemia population

Abstract

Provided is a method for rearing artemias in a feeding water tank, wherein a feed containing a mollusk-derived feed is poured into the feeding water tank. Provided is a feed for artemia that contains a mollusk-derived feed. Provided is an artemia which satisfies at least one of the following requirements at 6 days of age: body width being greater than 0.183 mm; body height being greater than 0.179 mm; and volume greater than 0.099 mm3.

Description

Artemia cultivation method, Artemia diet, Artemia and Artemia population

[0001] The present disclosure relates to methods for cultivating Artemia, Artemia diets, Artemia, and Artemia populations.

[0002] Various techniques have been developed for culturing aquatic organisms. For example, Artemia, a small crustacean, is used as a biological feed suitable for small aquatic organisms such as juvenile fish (see, for example, Patent Document 1).

[0003] Japanese Unexamined Patent Publication No. 126922 / 1983

[0004] When Artemia is used as a live feed for aquatic organisms, the Artemia may be cultivated in parallel with the cultivation of the aquatic organisms. In this case, the Artemia used as feed must also grow in size in accordance with the growth of the aquatic organisms. However, it is not easy to stably cultivate Artemia of an appropriate size depending on the amount and growth rate of the aquatic organisms being cultivated. The present disclosure has been made in view of the above, and aims to provide a technique for stably cultivating Artemia of an appropriate size, as well as Artemia and Artemia populations cultivated based on this technique.

[0005] The present disclosure provides the following: [1] A method for cultivating Artemia in a breeding tank, comprising introducing a feed containing a mollusk-derived feed into the breeding tank. [2] The method for cultivating Artemia according to [1], wherein the mollusk-derived feed contains 5% to 100% by mass of a mollusk-derived component. [3] The method for cultivating Artemia according to [1] or [2], wherein the mollusk-derived feed is derived from a shellfish. [4] The method for cultivating Artemia according to any of [1] to [3], wherein the mollusk-derived feed is a powder. [5] The method for cultivating Artemia according to any of [1] to [4], wherein the mollusk-derived feed contains particles, the average particle size of which is 10 μm to 300 μm. [6] A method for cultivating Artemia in a breeding tank, wherein feed introduced into the breeding tank contains at least one component selected from the group consisting of glycogen, zinc, taurine, and protein, and when the feed contains glycogen, the glycogen content is 1.1% by mass to 27.6% by mass on a total mass basis, and when the feed contains zinc, the zinc content is 5.0 x 10 on a total mass basis. －３ Mass%~251.1×10 －３% by mass, and when the feed contains taurine, the taurine content is 0.01% to 6.9% by mass based on the total amount, and when the feed contains protein, the protein content is 38.7% to 88.4% by mass based on the total amount. [7] The Artemia cultivating method of any of [1] to [6], in which the feed is supplied to the breeding tank until the Artemia reach 25 days of age. [8] Feed for Artemia comprising a mollusk-derived feed. [9] The Artemia feed of [8], in which the mollusk-derived feed contains 5% to 100% by mass of a mollusk-derived component.

[10] The Artemia feed of [8] or [9], in which the mollusk-derived feed is derived from a shellfish.

[11] The Artemia feed of

[10] , in which the mollusk-derived feed is powder.

[12] The Artemia feed of any of [8] to

[11] , wherein the mollusk-derived feed contains particles having an average particle size of 10 μm to 300 μm.

[13] The mollusk-derived feed contains at least one component selected from the group consisting of glycogen, zinc, taurine, and protein, and when the mollusk-derived feed contains glycogen, the glycogen content is 1.1% by mass to 27.6% by mass on a total mass basis, and when the mollusk-derived feed contains zinc, the zinc content is 5.0 × 10 on a total mass basis. －３ Mass%~251.1×10 －３

[14] The Artemia feed according to any one of [8] to

[12] , wherein, when the mollusk-derived feed contains the taurine, the taurine content is 0.01% to 6.9% by mass on a total mass basis, and when the mollusk-derived feed contains the protein, the protein content is 38.7% to 88.4% by mass on a total mass basis. ３

[15] Artemia satisfying at least one of the above criteria:

[15] Artemia having an oleic acid content of 22% by mass or more in its fatty acid composition;

[16] Artemia having an arachidonic acid content of 5% by mass or more in its fatty acid composition;

[17] Artemia containing at least one component selected from the group consisting of zinc and taurine in a greater amount than Artemia fed microalgae;

[18] Artemia having a digestive tract width / body length ratio of 0.03 or more;

[19] Artemia having a body length of 2 mm or more, and satisfying the following mathematical formula (1) where x is the body length and y is the ratio of the body width to the body length: y≧−0.0346x+0.1501 (1);

[20] Artemia having a population of 100 or more, and at 6 days of age, having an average body width of 0.205 mm or more, an average body height of 0.204 mm or more, and an average volume of 0.118 mm or more; ３

[21] The Artemia population according to

[20] , wherein the Artemia population is Artemia reared in the same environment.

[22] The Artemia population according to either

[20] or

[21] , wherein the Artemia population is Artemia reared on the same feed.

[0006] According to the present disclosure, there are provided techniques for stably cultivating artemia of an appropriate size, as well as artemia and artemia populations cultivated based on these techniques.

[0007] Fig. 1(A) is a plan view of Artemia, and Fig. 1(B) is a side view of Artemia. Fig. 1(A) and Fig. 1(B) are diagrams for explaining the body length, body width, and body height of Artemia. Fig. 2 is a diagram showing the results of Evaluation 2, showing the ratio of body width to body length of Artemia during rearing on each diet.

[0008] In this specification, numerical ranges indicated using "to" indicate ranges that include the numerical values ​​before and after the range as the minimum and maximum values, respectively. In this specification, when a composition contains multiple substances corresponding to each component, the amount of each component in the composition refers to the total amount of the multiple substances present in the composition, unless otherwise specified. In this specification, the terms "equal to or less than" or "less than" in relation to percentages include 0%, i.e., "not contained," unless a lower limit is specifically stated, or refer to a range that includes a value that is undetectable by current means.

[0009] [Artemia Cultivation Method] The Artemia cultivation method according to the present disclosure is a method for cultivating Artemia in a breeding tank, comprising introducing a mollusk-derived feed into the breeding tank. Introducing a mollusk-derived feed during Artemia cultivation can promote Artemia growth. In this specification, growth includes an increase in one or more of body length, body width, and volume. Specifically, when comparing Artemia of the same age, Artemia cultivated using a feed containing a mollusk-derived feed can be grown larger than Artemia cultivated using other feeds. In the present disclosure, increasing the size of Artemia includes an increase in body length and body width. Conventionally, microalgae (e.g., Nannochloropsis, Chlamydomonas) have been used as feed for Artemia cultivation. Nutritional enrichment using organic feed has also been considered to increase the size of Artemia. In contrast, including a mollusk-derived feed in the feed can promote Artemia growth. Furthermore, by promoting the growth of large Artemia, it becomes possible to stably cultivate Artemia of a certain size. Therefore, when Artemia is used as a feed, it becomes possible to cultivate a stable amount of Artemia, and it becomes possible to stably use Artemia as a live feed.

[0010] Details are explained below.

[0011] Artemia is the genus name of the Arthropoda, subphylum Crustacea, class Branchiopoda, subclass Salsostrata, order Anostraca, family Artemia. "Artemia" described below refers to various organisms included in the genus Artemia.

[0012] Artemia may be used as feed for raising larvae of aquatic organisms such as fish or cephalopods. Therefore, Artemia may be cultivated in a breeding tank separate from the aquatic organisms to be raised. In the following embodiment, a case where Artemia is cultivated in such a breeding tank will be described. However, the Artemia according to the present disclosure is not limited to being used as feed for aquatic organisms.

[0013] In this specification, the process of raising Artemia from hatching to adulthood is referred to as "raising" Artemia.

[0014] Typically, when cultivating Artemia, saltwater with a salinity of 1.0% to 4.0% by mass is stored in a breeding tank, and Artemia are introduced and raised therein. Seawater may be used as the saltwater. In this case, in the Artemia cultivating method according to the present disclosure, a feed containing a mollusk-derived feed is introduced into the breeding tank in which Artemia are cultivated.

[0015] Mollusk-derived feed is feed containing a mollusk-derived component. Mollusk-derived feed may be, for example, shellfish. "Molluscs" in this specification refer to animals belonging to the phylum Mollusca. The phylum Mollusca is classified into nine classes: Grossogastric, Caudata, Polyplacophora, Monoplacophora, Bivalvia, Diplopoda, Gastropoda, Polyplacophora, and Cephalopoda. "Shellfish" in this specification refer to animals belonging to five of the above eight classes: Monoplacophora, Bivalvia, Diplopoda, Polyplacophora, and Gastropoda.

[0016] The type of shellfish used in the mollusk-derived feed is not particularly limited, and for example, scallops, oysters, etc. can be used. Furthermore, multiple shellfish-derived products may be used as the feed, or a feed made from mollusks other than shellfish may be used as the mollusk-derived feed. When shellfish are used, the content of at least one component selected from the group consisting of glycogen, zinc, taurine, and protein may be higher than that of other mollusks. These components may contribute to the growth of larger Artemia. Therefore, by using shellfish as the mollusk-derived feed, it is possible to stably cultivate Artemia of an appropriate size.

[0017] Furthermore, the entire mollusk may be used as feed, or only a portion thereof may be used as feed. For example, in the case of shellfish, the entire mollusk, including the shell, may be used as feed, or only the parts other than the shell may be used as feed. When shellfish in mollusk-derived feed contain shells, Artemia ingest inorganic components such as calcium carbonate and protein contained in the shell, which may promote the growth of Artemia. By feeding calcium carbonate to Artemia, calcium, a component of bone, can be supplied to aquatic organisms that use Artemia as a live feed. This promotes bone growth in aquatic organisms, allowing them to increase their body length.

[0018] The mollusk-derived feed may be derived from shellfish. The mollusk-derived feed may be liquid or solid. Examples of liquids include a fluid produced by finely grinding mollusks and a liquid extracted by subjecting mollusks to a predetermined treatment. Liquid feed may also be prepared by diluting finely ground mollusks with water. Mollusks may also be finely ground in a mixer or the like, and an extract may be extracted as a liquid by filtering through a mesh. Frozen mollusks may also be thawed before use.

[0019] On the other hand, examples of solids include powders that are easy to ingest by Artemia that have a small body length after hatching. Examples of "powders" include particles with an average particle size of 10 μm to 300 μm. The average particle size may be 10 μm to 200 μm, or 10 μm to 100 μm. When the average particle size is within the above range, it is easy to ingest even for larvae that are approximately 0 to 3 days old after hatching. Furthermore, "powders" may be composed of particles with particle sizes within a predetermined range. The particle size of the particles contained in the powder may have a lower limit of 3 μm, 5 μm, 10 μm, or 20 μm. Furthermore, the upper limit of the particle size may be 300 μm, 200 μm, 100 μm, 50 μm, or 30 μm.

[0020] The particle size of the powder can be measured using an image analyzer VHX-900 (KEYENSE Corporation). The average particle size can be calculated based on the particle size measurement results. For example, for particles with an average particle size of 10 μm to 300 μm, the particle sizes of approximately 400 particles are measured using an image analyzer. The average particle size can be determined by calculating the arithmetic mean of the measured values. Here, the particle size is the diameter if each particle is spherical, or the length of the diagonal if each particle is rectangular. In other words, the diameter of the circumscribed circle of a particle displayed in a two-dimensional image is the particle size. The average particle size of the particles contained in the powder can be adjusted by separating them into multiple groups using sieving or other methods. Powders separated in this way and adjusted to a predetermined average particle size range can be used as feed. Regarding shellfish, powdering is suitable when using the shells as feed. In other words, powders can be used as mollusk-derived feed.

[0021] The mollusk-derived feed may contain 5% to 100% by mass, 10% to 100% by mass, 20% to 100% by mass, 30% to 100% by mass, 40% to 100% by mass, 50% to 100% by mass, 60% to 100% by mass, or 70% to 100% by mass of a mollusk-derived component, or 5% to 90% by mass, 5% to 80% by mass, 5% to 70% by mass, 5% to 60% by mass, or 5% to 50% by mass. The lower limit of the content of the mollusk-derived component may be 5% by mass or more, 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, or 70% by mass or more. The upper limit of the content of the mollusk-derived component may be 100% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, or 50% by mass or less. Examples of components other than the mollusk-derived component in the mollusk-derived feed include water, sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, calcium sulfate, calcium chloride, feeding attractants such as krill meal and squid liver meal, and fish oil. When the proportion of the mollusk-derived component is high, the growth of Artemia may be promoted by the components specific to mollusks.

[0022] In addition, when the feed put into the Artemia breeding tank contains other than mollusk-derived feed, the other feed may include feed that has traditionally been known as Artemia feed, such as algae feed, soybean flour, fish meal, etc.

[0023] Furthermore, a feed containing the mollusk-derived feed may contain a higher amount of a specific component than feed conventionally used for Artemia. Specifically, the Artemia cultivation method according to the present disclosure is a method for cultivating Artemia in a breeding tank, wherein the feed introduced into the breeding tank may contain at least one component selected from the group consisting of glycogen, zinc, taurine, and protein. Furthermore, when the feed contains glycogen, the glycogen content may be 1.1% to 27.6% by mass, 5% to 27.6% by mass, 10% to 27.6% by mass, 16% to 27.6% by mass, 1.1% to 22% by mass, 1.1% to 16% by mass, or 1.1% to 10% by mass, based on the total mass. The lower limit of the glycogen content may be 1.1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass or more, 10% by mass or more, 13% by mass or more, 16% by mass or more, 19% by mass or more, 22% by mass or more, or 25% by mass or more. The upper limit of the glycogen content may be 27.6% by mass or less, 25% by mass or less, 22% by mass or less, 19% by mass or less, 16% by mass or less, 13% by mass or less, 10% by mass or less, 8% by mass or less, 5% by mass or less, or 3% by mass or less.

[0024] In addition, when the feed contains the zinc, the content of the zinc is 5.0 × 10 based on the total amount. －３ Mass%~251.1×10 －３ Mass%, 10.0×10 －３ Mass%~251.1×10 －３ Mass%, 25.8×10 －３ Mass%~251.1×10 －３ Mass%, 50×10 －３ Mass%~251.1×10 －３ Mass%, 100×10 －３ Mass%~251.1×10 －３ Mass%, 150×10 －３ Mass%~251.1×10 －３ Mass%, 25.8×10 －３ Mass%~200×10 －３ Mass%, 25.8×10 －３ Mass%~150×10 －３ Mass%, 25.8×10 －３ Mass%~100×10－３ Mass%, 25.8×10 －３ Mass%~50×10 －３ Mass%, 5.0×10 －３ Mass%~200×10 －３ Mass%, 5.0×10 －３ Mass%~150×10 －３ Mass%, 5.0×10 －３ Mass%~100×10 －３ mass%, or 5.0 x 10 －３ Mass%~5.0×10 －３ The lower limit of the zinc content is 5.0 × 10 －３ Mass% or more, 10.0×10 －３ Mass% or more, 25.8×10 －３ Mass% or more, 50 x 10 －３ Mass% or more, 100×10 －３ mass% or more, or 150 x 10 －３ The upper limit of the zinc content is 251.1 × 10 －３ Mass% or less, 200×10 －３ Mass% or less, 150×10 －３ Mass% or less, 100×10 －３ mass% or less, or 50 x 10 －３ It may be % by mass or less.

[0025] When the feed contains taurine, the taurine content may be 0.01% to 6.9% by mass, 0.02% to 6.9% by mass, 1.2% to 6.9% by mass, 3% to 6.9% by mass, 5% to 6.9% by mass, 1.2% to 5% by mass, 1.2% to 3% by mass, 0.01% to 5% by mass, or 0.02% to 3% by mass, based on the total amount. The lower limit may be 0.01% by mass or more, 0.02% by mass or more, 1.2% by mass or more, 3% by mass or more, or 5% by mass or more. The upper limit may be 6.9% by mass or less, 5% by mass or less, or 3% by mass or less.

[0026] When the feed contains the protein, the protein content may be 38.7% to 88.4% by mass, 45% to 88.4% by mass, 50% to 88.4% by mass, 60% to 88.4% by mass, 38.7% to 80% by mass, 38.7% to 70% by mass, 38.7% to 60% by mass, 38.7% to 50% by mass, or 38.7% to 40% by mass, based on the total mass. The lower limit of the protein content may be 38.7% by mass or more, 45% by mass or more, 50% by mass or more, or 60% by mass or more. The upper limit of the protein content may be 88.4% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, 50% by mass or less, or 40% by mass or less.

[0027] Cultivating Artemia using a feed containing the above-mentioned components can promote the growth of Artemia. Specifically, when Artemia of the same age are compared, Artemia raised using the above-mentioned feed can grow larger than Artemia raised using other feeds. Conventionally, feeds derived from microalgae are used for raising Artemia, and nutritional enrichment can be performed using, for example, organic feed. In contrast, when a mollusk-derived feed is included in the feed, the growth of Artemia can be promoted without nutritional enrichment. Therefore, it is possible to stably cultivate Artemia of an appropriate size.

[0028] The feed containing the mollusk-derived feed is preferably administered until the Artemia reach 25 days of age, but may be administered after 25 days of age. In the present disclosure, "age" refers to the number of days elapsed since hatching, with the hatching day being considered as day 0. From hatching until 25 days of age, Artemia grow as the days increase. By cultivating Artemia using a feed containing the mollusk-derived feed during this period, the Artemia can be made larger.

[0029] The feed containing the mollusk-derived feed may be administered only during a portion of the period up to 25 days of age of Artemia. For example, the feed containing the mollusk-derived feed may be used only from 0 to 15 days of age, or only from 0 to 10 days of age. Furthermore, the timing of administering the feed from 0 to 25 days of age may be, for example, such that the feed containing the mollusk-derived feed is administered only during some of the multiple feed administrations. Specifically, the feed containing the mollusk-derived feed may be administered once a day, once every two days, or once every three days. However, the administration schedule is not limited to these.

[0030] [Artemia Feed] The Artemia feed according to the present disclosure may contain the mollusk-derived feed described above.

[0031] Mollusk-derived feed is feed containing a mollusk-derived component. The mollusk-derived feed may be, for example, shellfish. In this specification, "mollusk" refers to animals belonging to the phylum Mollusca. The phylum Mollusca is classified into nine classes: Grossogastric, Caudata, Polyplacophora, Monoplacophora, Bivalvia, Diplopoda, Gastropoda, Polyplacophora, and Cephalopoda. In this specification, "shellfish" refers to animals belonging to five of the above eight classes: Monoplacophora, Bivalvia, Diplopoda, Polyplacophora, and Gastropoda.

[0032] The type of shellfish used in the mollusk-derived feed is not particularly limited, and for example, scallops, oysters, etc. can be used. Furthermore, multiple shellfish-derived products may be used as the feed, or a feed made from mollusks other than shellfish may be used as the mollusk-derived feed. When shellfish are used, the content of at least one component selected from the group consisting of glycogen, zinc, taurine, and protein may be higher than that of other mollusks. These components may contribute to the growth of larger Artemia. Therefore, by using shellfish as the mollusk-derived feed, it is possible to stably cultivate Artemia of an appropriate size.

[0033] Furthermore, the entire mollusk may be used as feed, or only a portion thereof may be used as feed. For example, in the case of shellfish, the entire shell may be used as feed, or parts other than the shell may be used as feed. When shellfish in mollusk-derived feed are included, Artemia ingest inorganic components such as calcium carbonate and protein contained in the shell, which may promote Artemia growth. By feeding calcium carbonate to Artemia, calcium, a component of bone, can be supplied to aquatic organisms that use Artemia as a live feed. This promotes bone growth in aquatic organisms, allowing them to increase their body length.

[0034] The mollusk-derived feed may be derived from shellfish. The mollusk-derived feed may be liquid or solid. Examples of liquids include a fluid produced by finely grinding mollusks, and a liquid extracted by subjecting mollusks to a predetermined treatment. Furthermore, for example, a liquid feed may be prepared by diluting finely ground mollusks with water. Furthermore, a liquid extract may be extracted from a mollusk that has been finely ground using a mixer or the like by filtering through a mesh. Incidentally, frozen mollusks may be thawed before use.

[0035] On the other hand, examples of solids include powders that are easy to ingest by Artemia that have a small body length after hatching. Examples of "powder" include particles with an average particle size of 10 μm to 300 μm. The average particle size may be 10 μm to 200 μm, or 10 μm to 100 μm. When the average particle size is within the above range, it is easy to ingest even for larvae that are about 0 to 3 days old immediately after hatching. In addition, when shell parts of shellfish are also used as feed, it is suitable to make them into powder. In other words, powder may be used as a mollusk-derived feed.

[0036] The mollusk-derived feed may contain 5% to 100% by mass, 10% to 100% by mass, 20% to 100% by mass, 30% to 100% by mass, 40% to 100% by mass, 50% to 100% by mass, 60% to 100% by mass, or 70% to 100% by mass of the mollusk-derived component, or 5% to 90% by mass, 5% to 80% by mass, 5% to 70% by mass, 5% to 60% by mass, or 5% to 50% by mass. The lower limit of the content of the mollusk-derived component may be 5% by mass or more, 10% by mass or more, 20% by mass or more, 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, or 70% by mass or more. The upper limit of the content of the mollusk-derived component may be 100% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, or 50% by mass or less. Examples of components other than the mollusk-derived component in the mollusk-derived feed include water, sodium chloride, potassium chloride, magnesium chloride, magnesium sulfate, calcium sulfate, calcium chloride, feeding attractants such as krill meal and squid liver meal, and fish oil. When the proportion of the mollusk-derived component is high, the growth of Artemia may be promoted by the components specific to mollusks.

[0037] In addition, when the feed put into the Artemia breeding tank contains other than mollusk-derived feed, the other feed may include feed that has traditionally been known as Artemia feed, such as algae feed, soybean flour, fish meal, etc.

[0038] Furthermore, a feed containing the above-mentioned mollusk-derived feed may be a feed containing a higher amount of a specific component than feed conventionally used for Artemia. That is, the Artemia feed according to the present disclosure is used for cultivating Artemia in a breeding tank. The Artemia feed introduced into the breeding tank may contain at least one component selected from the group consisting of glycogen, zinc, taurine, and protein. When the feed contains glycogen, the glycogen content may be 1.1% to 27.6% by mass on a total mass basis. When the feed contains zinc, the zinc content may be 25.8 x 10 －３ Mass%~251.1×10－３ When the feed contains the taurine, the taurine content may be 1.2% to 6.9% by mass based on the total mass. When the feed contains the protein, the protein content may be 38.7% to 88.4% by mass based on the total mass.

[0039] When the feed contains glycogen, the glycogen content may be 1.1% by mass to 27.6% by mass, 5% by mass to 27.6% by mass, 10% by mass to 27.6% by mass, 16% by mass to 27.6% by mass, 1.1% by mass to 22% by mass, 1.1% by mass to 16% by mass, or 1.1% by mass to 10% by mass, based on the total mass. The lower limit of the glycogen content may be 1.1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass or more, 10% by mass or more, 13% by mass or more, 16% by mass or more, 19% by mass or more, 22% by mass or more, or 25% by mass or more. The upper limit of the glycogen content may be 27.6% by mass or less, 25% by mass or less, 22% by mass or less, 19% by mass or less, 16% by mass or less, 13% by mass or less, 10% by mass or less, 8% by mass or less, 5% by mass or less, or 3% by mass or less.

[0040] In addition, when the feed contains the zinc, the content of the zinc is 25.8 × 10 －３ Mass%~251.1×10 －３ Mass%, 50×10 －３ Mass%~251.1×10 －３ Mass%, 100×10 －３ Mass%~251.1×10 －３ Mass%, 150×10 －３ Mass%~251.1×10 －３ Mass%, 25.8×10 －３ Mass%~200×10 －３ Mass%, 25.8×10 －３ Mass%~150×10 －３ Mass%, 25.8×10 －３ Mass%~100×10 －３ mass%, or 25.8 x 10 －３ Mass%~50×10 －３ The lower limit of the zinc content is 25.8 × 10 －３ Mass% or more, 50 x 10－３ Mass% or more, 100×10 －３ mass% or more, or 150 x 10 －３ The upper limit of the zinc content is 251.1 × 10 －３ Mass% or less, 200×10 －３ Mass% or less, 150×10 －３ Mass% or less, 100×10 －３ mass% or less, or 50 x 10 －３ It may be % by mass or less.

[0041] When the feed contains taurine, the taurine content may be 1.2% by mass to 6.9% by mass, 3% by mass to 6.9% by mass, 5% by mass to 6.9% by mass, 1.2% by mass to 5% by mass, or 1.2% by mass to 3% by mass, based on the total amount. The lower limit of the taurine content may be 1.2% by mass or more, 3% by mass or more, or 5% by mass or more. The upper limit of the taurine content may be 6.9% by mass or less, 5% by mass or less, or 3% by mass or less.

[0042] When the feed contains the protein, the protein content may be 38.7% to 88.4% by mass, 45% to 88.4% by mass, 50% to 88.4% by mass, 60% to 88.4% by mass, 38.7% to 80% by mass, 38.7% to 70% by mass, 38.7% to 60% by mass, 38.7% to 50% by mass, or 38.7% to 40% by mass, based on the total mass. The lower limit of the protein content may be 38.7% by mass or more, 45% by mass or more, 50% by mass or more, or 60% by mass or more. The upper limit of the protein content may be 88.4% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass or less, 50% by mass or less, or 40% by mass or less.

[0043] Cultivating Artemia using a feed containing the above-mentioned components can promote the growth of Artemia. Specifically, when Artemia of the same age are compared, Artemia raised using the above-mentioned feed can grow larger than Artemia raised using other feeds. Conventionally, feeds derived from microalgae are used for raising Artemia, and nutritional enrichment can be performed using, for example, organic feed. In contrast, when a mollusk-derived feed is included in the feed, the growth of Artemia can be promoted without nutritional enrichment. Therefore, it is possible to stably cultivate Artemia of an appropriate size.

[0044] [Artemia] The Artemia according to the present disclosure is Artemia cultivated by ingesting the above-mentioned feed. The mollusk-derived feed may remain in the digestive tract of the Artemia. In this case, the mollusk-derived feed remaining in the digestive tract is also considered to be part of the Artemia and is subjected to component analysis of the Artemia.

[0045] Artemia fed the mollusk-derived feed according to the present disclosure may contain more zinc than Artemia fed microalgae. When the zinc is contained, the zinc content is 4.1 x 10 on a total basis. －３ Mass%~40.2×10 －３ Mass, 10×10 －３ Mass%~40.2×10 －３ Mass%, 15×10 －３ Mass%~40.2×10 －３ Mass%, 20×10 －３ Mass%~40.2×10 －３ Mass%, 25×10 －３ Mass%~40.2×10 －３ Mass%, 30×10 －３ Mass%~40.2×10 －３ Mass%, 35×10 －３ Mass%~40.2×10 －３ Mass%, 4.1×10 －３ Mass%~35×10 －３ Mass%, 4.1×10 －３ Mass%~30×10 －３ Mass%, 4.1×10 －３ Mass%~25×10－３ Mass%, 4.1×10 －３ Mass%~20×10 －３ Mass%, 4.1×10 －３ Mass%~15×10 －３ mass%, or 4.1 x 10 －３ Mass%~10×10 －３ The lower limit of the zinc content may be 4.1 × 10 based on the total amount. －３ Mass% or more, 10×10 －３ Mass% or more, 15 x 10 －３ Mass% or more, 20×10 －３ Mass% or more, 25 x 10 －３ Mass% or more, 30×10 －３ mass% or more, or 35 x 10 －３ The upper limit of the zinc content is 40.2 × 10 －３ Mass% or less, 35×10 －３ Mass% or less, 30×10 －３ Mass% or less, 25×10 －３ Mass% or less, 20×10 －３ Mass% or less, 15×10 －３ mass% or less, or 10 x 10 －３ It may be % by mass or less.

[0046] Artemia fed the mollusk-derived feed according to the present disclosure may contain more taurine than Artemia fed microalgae. When the Artemia contains taurine, the taurine content may be 0.1% to 1.1% by mass, 0.2% to 1.1% by mass, 0.5% to 1.1% by mass, 0.8% to 1.1% by mass, 0.1% to 0.8% by mass, 0.1% to 0.5% by mass, or 0.1% to 0.2% by mass, based on the total mass. The lower limit of the taurine content may be 0.1% by mass or more, 0.2% by mass or more, 0.5% by mass or more, or 0.8% by mass or more, based on the total mass. The upper limit of the taurine content may be 1.1% by mass or less, 0.8% by mass or less, 0.5% by mass or less, or 0.2% by mass or less. In one aspect, the taurine contained in the Artemia may be increased by, for example, 10%, 20%, 30%, 50%, 70%, 200%, 300%, 400%, or 500% compared to before feeding.

[0047] Artemia that satisfy any of the above conditions are likely to grow to a size suitable for use as feed for cultivating aquatic organisms such as fish or cephalopods. Therefore, Artemia that satisfy any of the above conditions contributes to the stable cultivation of Artemia of an appropriate size suitable for continuous use as feed for aquatic organisms. Furthermore, such Artemia are suitable for use as live feed.

[0048] The body length of Artemia according to the present disclosure at 6 days of age may be greater than 2.2 mm and less than 6.0 mm, greater than 2.3 mm and less than 6.0 mm, greater than 2.4 mm and less than 6.0 mm, greater than 2.5 mm and less than 6.0 mm, greater than 2.6 mm and less than 6.0 mm, greater than 2.7 mm and less than 6.0 mm, greater than 2.8 mm and less than 6.0 mm, greater than 2.9 mm and less than 6.0 mm, greater than 3.0 mm and less than 6.0 mm, greater than 2.2 mm and less than 5.5 mm, greater than 2.2 mm and less than 5.0 mm, greater than 2.2 mm and less than 4.5 mm, or greater than 2.2 mm and less than 4.0 mm. The lower limit of the body length at 6 days of age may be, for example, greater than 2.2 mm, greater than 2.3 mm, greater than 2.4 mm, greater than 2.5 mm, greater than 2.6 mm, greater than 2.7 mm, greater than 2.8 mm, greater than 2.9 mm, or greater than 3.0 mm. The upper limit of the body length at 6 days of age may be, for example, less than 6.0 mm, less than 5.5 mm, less than 5.0 mm, less than 4.5 mm, or less than 4.0 mm.

[0049] The body width of the Artemia at 6 days of age may be greater than 0.183 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.28 mm, greater than 0.2 mm and less than 0.26 mm, greater than 0.183 mm and less than 0.28 mm, or greater than 0.183 mm and less than 0.26 mm.

[0050] The body width of Artemia at 6 days of age may be greater than 0.183 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.3 mm, greater than 0.20 mm and less than 0.3 mm, greater than 0.183 mm and less than 0.28 mm, or greater than 0.183 mm and less than 0.26 mm. The lower limit of the body width at 6 days of age may be greater than 0.183 mm, greater than 0.19 mm, or greater than 0.2 mm. The upper limit of the body width at 6 days of age may be, for example, less than 0.3 mm, less than 0.28 mm, or less than 0.26 mm.

[0051] The body height of Artemia at 6 days of age may be greater than 0.179 mm and less than 0.3 mm, greater than 0.19 mm and less than 0.3 mm, greater than 0.2 mm and less than 0.3 mm, greater than 0.179 mm and less than 0.28 mm, or greater than 0.179 mm and less than 0.26 mm. The lower limit of the body height at 6 days of age may be greater than 0.179 mm, greater than 0.19 mm, or greater than 0.2 mm. The upper limit of the body height at 6 days of age may be less than 0.3 mm, less than 0.28 mm, or less than 0.26 mm.

[0052] The volume of Artemia at 6 days of age was 0.099 mm ３ Ultra-thin and 0.360mm ３ Less than 0.105 mm ３ Ultra-thin and 0.360mm ３ Less than 0.11 mm ３ Ultra-thin and 0.360mm ３ Less than 0.099 mm ３ Ultra-thin and 0.340mm ３ Less than or equal to 0.099 mm ３ Ultra-thin and 0.320mm ３ The lower limit of the volume at 6 days of age is 0.099 mm ３ It may be larger than 0.105 mm, and may be larger than 0.11 mm ３ The upper limit of the volume at 6 days of age is, for example, 0.360 mm ３ Less than 0.340 mm ３ Less than or equal to 0.320 mm ３ It may be less than.

[0053] The body length, body width, body height, and volume may satisfy at least one of the above dimensions. The body length is L1 shown in Figure 1(A), the body width is L2 shown in Figure 1(A), and the body height is L3 shown in Figure 1(B). The body width and body height can be determined by measuring the border between the head side and the tail side of the abdomen where pigment is present.

[0054] Furthermore, the ratio of gut width to body length of Artemia according to the present disclosure, i.e., gut width / body length, may be greater than 0.027 and less than 0.05, greater than 0.028 and less than 0.05, greater than 0.029 and less than 0.05, greater than 0.03 and less than 0.05, greater than 0.03 and less than 0.05, or greater than 0.03 and less than 0.05. The lower limit of the gut width / body length of Artemia may be greater than 0.027, greater than 0.028, greater than 0.029, greater than 0.03, greater than 0.03, or greater than 0.031. The upper limit of the gut width / body length of Artemia may be less than 0.05. Artemia raised using the above-mentioned diet tend to have a larger gut width.

[0055] Artemia contains fatty acids including oleic acid and arachidonic acid. The content of oleic acid relative to the total fatty acids contained in Artemia, i.e., the content of oleic acid in the fatty acid composition of Artemia, may be 22% to 40% by mass, 25% to 40% by mass, 22% to 35% by mass, or 22% to 30% by mass. The lower limit of the oleic acid content may be 22% by mass or more, or 25% by mass or more. Artemia with such a sufficiently high oleic acid content can be suitably used as a live animal feed. The upper limit of the oleic acid content may be 40% by mass or less, 35% by mass or less, or 30% by mass or less.

[0056] The content of arachidonic acid relative to the total fatty acids contained in Artemia, i.e., the content of arachidonic acid in the fatty acid composition of Artemia, may be 5% by mass to 30% by mass, 5% by mass to 20% by mass, 5% by mass to 15% by mass, 8% by mass to 30% by mass, or 11% by mass to 30% by mass. The arachidonic acid content may be 5% by mass or more, 8% by mass or more, or 11% by mass or more. Artemia with such a sufficiently high arachidonic acid content can be suitably used as a biological feed. The arachidonic acid content may be 30% by mass or less, 20% by mass or less, or 15% by mass or less.

[0057] The total content of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia may be 25% to 50% by mass, 29% to 50% by mass, 32% to 50% by mass, 35% to 50% by mass, 25% to 45% by mass, 29% to 45% by mass, 32% to 45% by mass, or 35% to 45% by mass. The lower limit of the total content of oleic acid and arachidonic acid may be 25% by mass or more, 29% by mass or more, 32% by mass or more, or 35% by mass or more. Artemia with such a sufficiently high total content of oleic acid and arachidonic acid can be suitably used as a live animal feed. The upper limit of the total content of oleic acid and arachidonic acid may be 50% by mass or less, or 45% by mass or less.

[0058] The content of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia can be measured by analyzing the composition of lipids extracted from Artemia. Gas chromatography can be used for composition analysis.

[0059] The respective contents of oleic acid and arachidonic acid relative to the total fatty acids contained in Artemia can be increased by feeding Artemia a diet containing a predetermined amount of at least one component selected from the group consisting of glycogen, zinc, taurine, and protein, such as a mollusk-derived diet.

[0060] Furthermore, the Artemia according to the present disclosure may have a body length of 1.5 mm or more, and may satisfy the following mathematical formula (6) or (1) when the body length is x and the ratio of the body width is y. The body length of the Artemia may be 2.0 mm or more and less than 6.0 mm, 2.2 mm or more and less than 6.0 mm, 2.0 mm or more and less than 5.0 mm, 2.0 mm or more and less than 4.0 mm, or 2.2 mm or more and less than 5.0 mm. The lower limit of the body length of the Artemia may be 2.0 mm or more or 2.2 mm or more. The upper limit of the body length of the Artemia may be less than 6.0 mm, less than 5.0 mm, or less than 4.0 mm. y≧−0.0346x+0.1501 (6) y≧−0.0252x+0.136 (1)

[0061] [Artemia Population] When an Artemia population is an aggregation of multiple Artemia, the Artemia population according to the present disclosure has, when 100 or more individuals are measured, an average body width of 0.205 mm or more, an average body height of 0.204 mm or more, and an average volume of 0.118 mm or more at 6 days of age. ３ The rearing period may be 6 days or more after hatching, and may be, for example, 7 days, 8 days, 9 days, 10 days, 11 days, or 12 days. The period can be extended as long as rearing is possible, but since this increases production costs, the period may be 30 days or less, 20 days or less, or 15 days or less.

[0062] Such an Artemia population can be obtained by feeding the mollusk-derived feed during rearing. The mollusk-derived feed may be fed continuously throughout rearing, or immediately before use as a feed for aquatic organisms. The immediately before feeding period may be, for example, one or more of the following: 30 minutes, 30 minutes to 1 hour, 1 hour to 3 hours (excluding 1 hour), 3 hours to 5 hours (excluding 3 hours), 5 hours to 8 hours (excluding 5 hours), 8 hours to 12 hours (excluding 8 hours), or 12 hours to 24 hours (excluding 12 hours). An Artemia population that meets these conditions has a size suitable for use as a feed for cultivating aquatic organisms. Furthermore, because the Artemia population is larger overall than Artemia cultivated with conventional feed, the weight of feed required for aquatic organisms can be prepared with fewer individuals.

[0063] The number of Artemia individuals contained in the Artemia population may be 100 to 100 million, 200 to 100 million, 500 to 100 million, 1,000 to 100 million, 10,000 to 100 million, 10,000 to 50 million, 100,000 to 10 million, or 1,000,000 to 5,000,000. The lower limit of the number of Artemia individuals may be 100 or more, 200 or more, 500 or more, 1,000 or more, or 10,000 or more. There is no particular limit on the upper limit of the number of Artemia individuals contained in the Artemia population, and it may be, for example, 100 million or less, 50 million or less, 10 million or less, or 5 million or less.

[0064] All Artemia included in the Artemia population may be reared in the same environment, which includes, for example, not only the case where one rearing tank is used, but also the case where multiple rearing tanks of the same size are used in the same building, and the same type of feed is used at the same feeding timing.

[0065] All Artemia in the Artemia population may be grown on the same diet, which can reduce size variation among the Artemia in the Artemia population.

[0066] Although the embodiments of the present disclosure have been described above, the Artemia cultivating method, Artemia feed, Artemia, and Artemia population according to the present disclosure are not limited to the above embodiments.

[0067] [Examples and Comparative Examples] In the following evaluations, Artemia salina was purchased from ISC Co., Ltd. and used. 500 g of oyster powder (product name) was purchased from Netshosha.com and sieved through a 300 μm mesh sieve. Marine Omega A (product name) microalgae was purchased from Marine Tech Co., Ltd. The zinc, glycogen, taurine, and protein contents contained in the oyster powder used were as shown in Table 1. The zinc content was measured by ICP emission spectrometry, and the glycogen content was measured by the anthrone sulfate method. The taurine (amino acid) content was analyzed by pre-column derivatization high-performance liquid chromatography after pretreatment with hydrochloric acid hydrolysis. The protein content was the value listed in the product (Marine Omega A) instructions.

[0068]

[0069] (Evaluation 1) Two identical 200 L breeding tanks containing 2 million 0-day-old Artemia were prepared, and each tank was fed a different diet. The Artemia were reared in seawater with a salinity of 31-38‰, DO of 5.0 or higher, a water temperature of 28°C, and aeration of 1 L / min. One tank was fed oyster powder, while the other tank was fed microalgae.

[0070] The amounts of feed fed to the breeding tanks were as follows: 200 ml was fed per time to the breeding tank fed with microalgae as a satiation amount; 30 g was fed per time to the breeding tank fed with oyster powder as a satiation amount; Feeding was carried out twice a day in both breeding tanks at the same timing. Artemia were sampled at 0 and 6 days of age, and their movement was stopped by dripping boiling water with a dropper. Body length, body width, and body height were measured on a microscope image using an image analyzer VHX-900 (KEYENSE Corporation).

[0071] Body length, body width, and body height were measured at the lengths of L1, L2, and L3 shown in Figure 1. Body width and body height were measured at the boundary between the presence and absence of pigment, as this is where the boundary can be confirmed. Ten Artemia were selected from each breeding tank, and their body length, body width, and body height were measured. The volume was then calculated by multiplying body length by body width by body height. The average volume of the 10 individuals was then calculated. The results are shown in Table 2.

[0072] At 0 days of age, the volume of both Artemia fed with oyster powder and Artemia fed with microalgae was 0.015 mm ３ At 6 days old, the Artemia fed with microalgae had a size of 0.099 mm ３ On the other hand, the Artemia fed with oyster powder had a size of 0.118 mm ３ This is what happened.

[0073] It was confirmed that Artemia cultivated on oyster powder feed were larger in volume than Artemia cultivated on microalgae feed.

[0074]

[0075] (Evaluation 2) Two identical 200 L breeding tanks containing 2 million 0-day-old Artemia were prepared, and culture was performed by feeding each tank with a different feed. The breeding conditions were the same as those in Evaluation 1. Oyster powder was used as feed in one breeding tank, and microalgae was used as feed in the other. That is, one tank is an example, and the other is a comparative example.

[0076] The amount of feed fed to the breeding tanks was 200 ml per feeding, which was the satiation amount, for the comparative example breeding tank fed with microalgae. The satiation amount for the example breeding tank fed with oyster powder was 30 g per feeding. Feeding was conducted twice daily at the same timing for both the example and the comparative example. At 6 days of age, 20 Artemia were selected from the oyster powder-fed area and 13 from the microalgae-fed area in the breeding tanks. Their body length and body width were measured, and the body width / body length ratio was calculated. Individuals with a body length of 1.5 mm or greater were selected. The calculation results are shown in Table 3. The units for "body length" and "body width" in Table 3 are "mm." Based on these results, the measurement results for each individual were plotted in Figure 2, with body length on the horizontal axis and body width / body length on the vertical axis. Figure 2 also shows the results for individuals with a body length of less than 1.5 mm. The plotted data was approximated by a linear function and the results are also shown in FIG.

[0077]

[0078] Based on Figure 2, Artemia cultivated using oyster powder have a body length of 1.5 mm or more, and when the body length is x mm and the body width / body length is y, the following mathematical formula (1) can be satisfied: y ≧ -0.0232x + 0.1309 (1)

[0079] As a result of linear function approximation in the range of body length 1.5 m or more, the Artemia cultivated with oyster powder was approximated by the following formulas (2) and (3): y = -0.0232x + 0.1308 ... (2) R ２ = 0.8381 ... (3)

[0080] In addition, the Artemia cultivated with microalgae was approximated by the following formulas (4) and (5): y = -0.0404x + 0.1506 (4) R ２ = 0.6741 ... (5)

[0081] On the other hand, the range satisfying the following formula (6) could only be achieved by Artemia cultivated using oyster powder, but not by Artemia cultivated using microalgae. This tendency was particularly pronounced for Artemia with a body length of 2 mm or more. y≧−0.0346x+0.1501 (6)

[0082] (Evaluation 3) Two identical breeding tanks containing 2 million 0-day-old Artemia were prepared, and each tank was fed a different diet. The breeding conditions were the same as those in Evaluation 1. Oyster powder was used as the feed in one breeding tank, while microalgae was used in the other. That is, one tank was the Example and the other was the Comparative Example. The amount of feed provided to the breeding tanks was 200 ml per feeding, a satiation amount, for the Comparative Example breeding tank fed with microalgae. The amount of feed provided to the Example breeding tank fed with oyster powder was 30 g per feeding, a satiation amount. Both the Example and the Comparative Example were fed twice a day at the same timing. At 6 days of age, Artemia were collected from each breeding tank, lipids were extracted, and the fatty acid composition was analyzed using GC-FID. The measurement results for the proportions of oleic acid and arachidonic acid among the total fatty acids obtained are shown in Table 4.

[0083]

[0084] The oleic acid content of Artemia fed oyster powder was 25.91% by mass and that of Artemia fed microalgae was 20.36% by mass, while the arachidonic acid content of Artemia fed oyster powder was 11.61% by mass and that of Artemia fed microalgae was 3.57% by mass.

[0085] Therefore, it was confirmed that the Artemia fed with oyster powder had increased proportions of oleic acid and arachidonic acid compared to Artemia fed with microalgae. According to the present disclosure, there are provided a technology for stably cultivating Artemia of an appropriate size, as well as Artemia and Artemia populations cultivated based on this technology.

Claims

1. A method for cultivating Artemia in a breeding tank, comprising: A method for cultivating Artemia, comprising introducing a feed containing a mollusk-derived feed into the breeding tank.

2. The Artemia cultivation method according to claim 1, wherein the mollusk-derived feed contains 5% to 100% by mass of a mollusk-derived component.

3. The method for cultivating Artemia according to claim 1 or 2, wherein the mollusk-derived feed is derived from shellfish.

4. 3. The method for cultivating Artemia according to claim 1 or 2, wherein the mollusk-derived feed is in the form of a powder.

5. 3. The method for cultivating Artemia according to claim 1, wherein the mollusk-derived feed contains particles having an average particle size of 10 μm to 300 μm.

6. A method for cultivating Artemia in a breeding tank, comprising: the feed introduced into the breeding tank contains at least one component selected from the group consisting of glycogen, zinc, taurine, and protein; When the feed contains the glycogen, the content of the glycogen is 1.1% by mass to 27.6% by mass on a total mass basis, When the feed contains zinc, the zinc content is 5.0 x 10 －３ Mass%~251.1×10 －３ is mass %; When the feed contains the taurine, the content of the taurine is 0.01% by mass to 6.9% by mass on a total mass basis, When the feed contains the protein, the protein content is 38.7% by mass to 88.4% by mass based on the total mass.

7. The Artemia cultivating method according to claim 1 or 6, wherein the feed is added to the breeding tank until the Artemia reach 25 days of age.

8. Artemia diets, including mollusk-derived diets.

9. The Artemia feed according to claim 8, wherein the mollusk-derived feed contains 5% by mass to 100% by mass of a mollusk-derived component.

10. The Artemia feed according to claim 8 or 9, wherein the mollusk-derived feed is derived from a shellfish.

11. The Artemia feed of claim 10, wherein the mollusk-derived feed is in the form of a powder.

12. The Artemia feed according to claim 8 or 9, wherein the mollusk-derived feed contains particles having an average particle size of 10 μm to 300 μm.

13. The mollusk-derived feed contains at least one component selected from the group consisting of zinc and taurine, and when the mollusk-derived feed contains zinc, the zinc content is 5.0 x 10 based on the total amount. －３ Mass%~251.1×10 －３ 10. The Artemia feed according to claim 8 or 9, wherein the mollusk-derived feed contains taurine in an amount of 0.01% by mass to 6.9% by mass based on the total amount of the mollusk-derived feed.

14. At 6 days of age, the body width was greater than 0.183 mm, the body height was greater than 0.179 mm, and the volume was greater than 0.099 mm. ３ Artemia that meets at least one of the following criteria:

15. Artemia having an oleic acid content of 22% by mass or more in its fatty acid composition.

16. Artemia having an arachidonic acid content of 5% by mass or more in its fatty acid composition.

17. Artemia containing at least one component selected from the group consisting of zinc and taurine in a larger amount than Artemia fed with microalgae.

18. Artemia, in which the digestive tract width / body length is 0.03 or more.

19. An Artemia having a body length of 2 mm or more, which satisfies the following formula (1) when the body length is x and the ratio of the body width is y: y≧-0.0346x+0.1501...(1)

20. The number of individuals is 100 or more, and at 6 days of age, the average body width is 0.205 mm or more, the average body height is 0.204 mm or more, and the average volume is 0.118 mm or more. ３ An Artemia population that satisfies at least one of the above.

21. The Artemia population according to claim 20, wherein the Artemia population is Artemia reared in the same environment.

22. The Artemia population according to claim 20 or 21, wherein the Artemia population is Artemia grown on the same feed.

23. A method for cultivating Artemia as described in claim 1 or 2, wherein the feed containing the mollusk-derived feed is administered to the Artemia at day 0 in the breeding tank.

24. A method for cultivating Artemia as described in claim 1 or 2, wherein the feed containing the mollusk-derived feed is administered to the Artemia in the breeding tank at least once every three days from the age of 0 days to the age of 25 days.

25. A method for cultivating Artemia as described in claim 1 or 2, wherein the Artemia are daily administered the feed containing the mollusk-derived feed in the breeding tank until they reach at least 0 days of age to 6 days of age.