Centrifuge and collection device

Abstract

This centrifuge and collection device comprises: a centrifuge unit that holds a centrifuge tube for accommodating a specimen solution and centrifuges the specimen solution; a pickup unit that picks up a specimen container having a centrifuge layer; a light detection unit that detects a specific layer from the centrifuge layer in the tube; a layer detection dispensing unit that separately collects the specific layer; and a control unit that controls the operation of each unit.

Description

Centrifugal fractionator

[0001] The present invention relates to a centrifugal separation device that centrifuges a sample solution and separates a specific layer from multiple centrifuged layers, and more particularly to a centrifugal separation device that centrifuges whole blood and detects and separates a specific layer from multiple centrifuged layers.

[0002] The whole blood sample is centrifuged at approximately 1500 rpm in a centrifuge tube to separate the whole blood into multiple centrifugal layers. Before centrifugation, the whole blood is diluted and an anticoagulant (such as sodium citrate) is added.

[0003] As shown in Figure 14, the multiple centrifugal layers are, from top to bottom in the centrifuge tube T, an upper layer A, a centrifugal agent layer B containing an anticoagulant, etc., and a red blood cell layer C. The upper layer A is composed of an upper serum / plasma layer A1 and a lower buffy coat layer A2. The serum / plasma layer A1 is a mixed solution layer containing cancer cells, exosomes, proteins, etc. The buffy coat layer A2 is an immune cell solution layer, but its volume is a thin layer, accounting for no more than a few percent of the total volume.

[0004] Patent Document 1 proposes a sample preparation device equipped with a centrifuge. This sample preparation device includes a centrifugal separator 30, a transporter 40 for transporting centrifuge tubes 12, and a dispenser 50. The dispenser 50 is movable on a common transport shaft 41 with the transporter 40 (see Abstract, paragraph

[0024] and Figure 1). The transporter 40 also includes a gripper 434 for gripping the centrifuge tubes 12 (see paragraph

[0042] ).

[0005] Japanese Patent Application Laid-Open No. 2023-125975

[0006] The sample preparation device of Patent Document 1 does not describe detecting and separating a specific layer from multiple centrifugal separation layers. Therefore, the present invention provides a centrifugal separation device that detects and separates a specific layer from multiple centrifugal separation layers obtained by centrifuging a sample solution.

[0007] Each embodiment of the present invention is configured as follows: (Embodiment 1) A centrifugal separation device for separating a specific layer from a plurality of centrifugal separation layers, comprising: a centrifugation unit that accommodates a centrifugation container that accommodates a specimen solution and centrifuges the specimen solution, a pickup unit that picks up the centrifugation container having the multiple centrifugal separation layers formed by centrifugation of the specimen solution, a layer detection unit that detects the specific layer from the multiple centrifugal separation layers in the centrifugation container, a layer detection dispensing unit that separates the specific layer, and a control unit that controls the operation of each unit.

[0008] (Example 2) The centrifugal separation device according to Example 1, comprising a first support part that supports the pickup part, a second support part that supports the layer detection dispensing part, and a main support part that supports the first support part and the second support part. (Example 3) The centrifugal separation device according to Example 2, comprising a first lifting mechanism that raises and lowers the first support part, a second lifting mechanism that raises and lowers the second support part, a first support part lateral movement mechanism that moves the first support part in a first lateral direction, and a second support part lateral movement mechanism that moves the second support part in the first lateral direction. (Example 4) The centrifugal separation device according to Example 2, comprising a main body or main body wall that supports the main support part.

[0009] (Example 5) The centrifugal separation device according to Example 4, comprising a main support part moving mechanism that moves the main support part in a second lateral direction different from the first lateral direction. (Example 6) The centrifugal separation device according to Example 2, wherein the first support part is movably provided on a first side surface of the main support part, and the second support part is movably provided on a second side surface opposite to the first side surface of the main support part. (Example 7) The centrifugal separation device according to Example 2, wherein the first support part, the second support part, and the main support part are provided above a stage of a main body.

[0010] (Example 8) The centrifugal separation device according to Example 1, wherein the layer detection unit is a current detection unit that detects the specific layer based on the electrical conductivity of the multiple centrifugal separation layers, and / or a light detection unit that detects the specific layer based on light transmitted through the multiple centrifugal separation layers. (Example 9) The centrifugal separation device according to Example 8, wherein the current detection unit detects the current between a pair of electrodes provided in the layer detection dispensing unit. (Example 10) The centrifugal separation device according to Example 8, wherein the light detection unit includes a storage rack that stores multiple centrifugal separation containers that have been centrifuged, arranged in a line in the first horizontal direction, and the layer detection dispensing unit includes multiple dispensing nozzles arranged in a line in the first horizontal direction.

[0011] (Example 11) The centrifugal separation apparatus according to Example 1, comprising an extraction device that extracts a target substance from the specific layer dispensed from the layer detection dispensing unit. (Example 12) The centrifugal separation apparatus according to Example 11, wherein the extraction device comprises a plurality of shaking containers lined up in a row in the first horizontal direction, and a shaking mechanism that shakes the plurality of shaking containers. (Example 13) The centrifugal separation apparatus according to Example 1, further comprising a centrifugation dispensing unit that dispenses various reagents and / or solutions into the centrifugation containers. (Example 14) The centrifugal separation apparatus according to Example 13, comprising a consumables storage unit that stores consumables including the various reagents and / or solutions.

[0012] (Example 15) The centrifugal separation device according to Example 14, wherein the consumables storage unit stores the containers for the various reagents, the containers for the solutions, and / or the dispensing tips attached to the plurality of dispensing nozzles, arranged in a line in the first horizontal direction. (Example 16) The centrifugal separation device according to Example 1, wherein the pickup unit includes at least a pair of arms that grip the centrifugation containers.

[0013] The centrifugal separation device of the present invention can detect and separate a specific layer from a plurality of centrifuged layers obtained by centrifuging a sample solution.

[0014] 1. A left perspective view of a centrifugal separation device according to a first embodiment of the present invention. 2. A right perspective view of the centrifugal separation device of FIG. 1. 3. (a) Top view, (b) Front view, and (c) Side view of the centrifugal separation device of FIG. 1. 4. A longitudinal cross-sectional view showing a second dispensing nozzle and an electrode tip of the layer detection dispensing unit of FIG. 1. 5. A schematic diagram showing the use state of the layer detection dispensing unit and the optical detection unit of FIG. 4. 6. A cross-sectional view showing the optical detection unit of FIG. 5. 7. A block diagram of the centrifugal separation device of FIG. 1. 8. A perspective view showing the operation of the centrifugal separation device of FIG. 1. 9. A perspective view showing the operation of the pickup unit of FIG. 1. 10. A left perspective view of a centrifugal separation device according to a second embodiment of the present invention. 11. A perspective view showing the dispensing operation to the extraction device of FIG. 10. 12. An exploded perspective view of the extraction device of FIG. 10. 13. A perspective view showing the swinging operation of the extraction device of FIG. 10. 14. A cross-sectional view of a centrifuge tube showing the state after centrifuging whole blood.

[0015] Embodiments of the centrifugal separation device of the present invention will be described with reference to the drawings. While the present embodiments will be described using an example in which a specific layer (e.g., a buffy coat layer) is collected by centrifugation from a specimen solution (e.g., whole blood), the present invention is not limited thereto and can also be applied to collecting any layer that can be centrifuged from any specimen solution. The y and x directions in the present embodiments correspond to the first and second horizontal directions in the claims, respectively.

[0016] 1 and 2, a centrifugal separation device 1 according to a first embodiment will be described. The centrifugal separation device 1 includes a main body 10 having a stage 11, a centrifugal separation unit 20 disposed within the main body 10, a pickup unit 30 movable above the stage 11, a centrifugal separation dispensing unit 35 movable above the stage 11, a first support unit 40 supporting the pickup unit 30 and the centrifugal separation dispensing unit 35, a second support unit 50 supporting a layer detection dispensing unit 51 (FIG. 3) movable above the stage 11, a main support unit 60 supporting the first support unit 40 and the second support unit 50 above the stage 11, an optical detection unit 70 detecting a specific layer separated by centrifugation from whole blood, and a control unit 80 controlling the operation of each unit.

[0017] The main body 10 has a substantially horizontal stage 11, a consumables storage section 12 recessed from the stage 11, a main body wall section 13 extending upward from the rear surface of the stage 11, and a control section 80 that controls the operation of each section.

[0018] The centrifugal separation unit (centrifuge) 20 includes a cover 21 that covers the stage 11 side, and the cover 21 has an opening 21a. The pickup unit 30 and the centrifugal separation dispensing unit 35 can access the centrifugal separation unit 20 from above the stage 11 through the opening 21a. The centrifugal separation unit 20 rotatably holds a plurality of centrifugation tubes T (centrifugation containers). The centrifugal separation unit 20 includes a centrifuge motor 23 (FIG. 6). The tubes T contain a sample solution such as whole blood.

[0019] The pickup unit 30 includes at least one pair of arms 31 (FIG. 7) for gripping at least one tube T, and an arm drive motor 32 (FIG. 6) for driving the arms 31. The number of pairs of arms 31 may be multiple, preferably two pairs.

[0020] The centrifugal separator (centrifugal separator) 35 includes at least one first separator nozzle 36 (FIGS. 7 and 8) and a first suction / discharge mechanism 38 (FIG. 6) that performs suction and discharge from the first separator nozzle 36. Preferably, there may be one or more first separator nozzles 36. A separator tip 36a (FIG. 7) is detachably attached to the first separator nozzle 36. The first suction / discharge mechanism 38 includes a cylinder and an actuator that drives the cylinder.

[0021] The first support part 40 is provided on the first side surface 60a (FIGS. 1 and 3(a)) side of the main support part 60 so as to be movable in the y direction. The first support part 40 includes the pickup part 30 and the centrifugation dispensing part 35, and a first lifting mechanism 41 (FIG. 6) that raises and lowers the pickup part 30 and / or the centrifugation dispensing part 35. The pickup part 30 and the centrifugation dispensing part 35 are preferably raised and lowered integrally with the first support part 40 by the first lifting mechanism 41. The first lifting mechanism 41 is composed of, for example, an actuator.

[0022] The second support section 50 is provided on a second side surface 60b (FIGS. 2 and 3(a)) opposite the first side surface 60a of the main support section 40 so as to be movable in the y direction. The second support section 50 includes a layer detection dispensing section 51. The layer detection dispensing section 51 includes a plurality of second dispensing nozzles 51a arranged in a line in the y direction, a second lifting mechanism 58 (FIG. 6) that raises and lowers the second dispensing nozzles 51a, and a second suction and discharge mechanism 55 (FIG. 6) that performs suction and discharge of the second dispensing nozzles 51a. The plurality of second dispensing nozzles 51a can preferably be eight second dispensing nozzles 51a. Each of the plurality of second dispensing nozzles 51a can preferably be equipped with a pair of electrodes as shown in FIG. 4. The second suction and discharge mechanism 55 can be composed of, for example, a cylinder and an actuator that drives the cylinder. The second lifting mechanism 58 can be composed of, for example, an actuator.

[0023] The main support 60 is supported in a cantilevered manner by the main body wall 13 and extends from the main body wall 13 above the stage 11 in the y direction. The main body wall 13 or the main body 10 is provided with a main support movement mechanism 63 ( FIG. 6 ) that moves the main support 60 in the x direction. The main support movement mechanism 63 is composed of, for example, a motor and rails. The optical detection unit 70 is provided with a storage rack (storage recess) 72 that stores multiple tubes T in a row in the y direction, and multiple optical detection units 78 ( FIGS. 5 and 5A ) provided on the storage rack 72.

[0024] The consumables storage unit 12 is provided in the main body 10 and is open on its top surface (xy plane) facing the stage 1 and on the front surface (xz plane) of the main body 10. The consumables storage unit 12 houses a bottle 16 containing at least one reagent and / or solution and at least one consumables rack 14 containing various consumables. The various consumables may be, for example, a plurality of dispensing tips 36a attached to the first dispensing nozzle 36, a plurality of electrode tips 3 attached to the second dispensing nozzle 53, a plurality of containers, and / or a plurality of tubes T. The consumables rack 14 has a plurality of consumables arranged in a line in the y direction at the same intervals as the plurality of second dispensing nozzles 51a ( FIG. 3( c) ) arranged in a line in the y direction. Therefore, a plurality of second dispensing nozzles 51a can be attached to multiple dispensing tips or electrode tips at the same time, and can aspirate or discharge liquid from a plurality of tubes T at the same time.

[0025] The layer detection dispensing unit (layer detection dispensing device) 51 detects any centrifuged layer based on the difference in electrical conductivity between the centrifuged layers. As shown in Figures 4 and 5, the layer detection dispensing unit 51 is composed of a second dispensing nozzle 53 equipped with electrode terminals 56a and 56b, an electrode-equipped dispensing tip 59 attached to the first dispensing nozzle 51, a positive electrode 55a extending along the first dispensing tip 59, a negative electrode 55b extending along the dispensing tip 59, a positive terminal 56a electrically connected to the upper end of the positive electrode 55a, a negative terminal 56b electrically connected to the upper end of the negative electrode 55b, and a current detection unit 57 electrically connected via wiring to each of the positive terminal 56a and the negative terminal 56b. The dispensing tip 59 is preferably molded from resin so as to entirely cover the negative electrode 55b and the positive electrode 55a, except for the upper and lower ends of the negative electrode 55b and the positive electrode 55a. As a result, the dispensing tip 59 is integrated with the negative electrode 55b and the positive electrode 55a.

[0026] The current detection unit (current detection circuit) 57 measures the electrical conductivity between the centrifugal separation layers at the positive lower end 55a1 of the positive electrode 55a and the negative lower end 55b1 of the negative electrode 55b. During the electrical conductivity measurement, the control unit 80 moves the electrode-equipped second dispensing nozzle 53 equipped with the dispensing tip 59 in the vertical direction (preferably from top to bottom) at a speed that does not disturb the layer interface, preferably a constant speed. The control unit 80 detects a specific layer based on the difference in electrical conductivity between the centrifugal separation layers measured by the current detection unit 57.

[0027] The surfaces of the positive electrode 55a and the negative electrode 55b are covered with an insulating layer. A positive lower end 55a1 is provided at the lower end of the positive electrode 55a, with the conductive surface exposed and not covered by the insulating layer. A negative lower end 55b1 is provided at the lower end of the negative electrode 55b, with the conductive surface exposed and not covered by the insulating layer. The positive lower end 55a1 and the negative lower end 55b1 are located to the sides of the lower end 59a of the dispensing tip 59. More preferably, the positive lower end 55a1 and the negative lower end 55b1 are located at substantially the same height (for example, within a range of ±1 mm) as the lower end (opening) 59a of the dispensing tip 59.

[0028] 5 and 5A, the optical detection section (optical detection device) 70 includes a plurality of optical detection units 78 arranged on the outer surfaces of a plurality of tubes T. The tubes T are formed from a light-transmitting material (e.g., plastic, glass, etc.). The optical detection units 78 include an illumination section (a light-emitting element such as an LED) 78a that emits light toward the tubes T, a light-receiving section (a light-receiving element such as a photodiode) 78b that receives light transmitted through the tubes T from the illumination section 78a, a detection recess 78d that receives a portion of the tubes T, an optical detection housing 78e that houses the illumination section 78a and the light-receiving section 78b, and an optical detection lifting mechanism 78c that raises and lowers the optical detection section housing 78e along the tubes T. The optical detection lifting mechanism 78c can be, for example, an actuator that includes a cylinder and its drive motor.

[0029] 5A, by providing the illumination surface of the illumination unit 78a and the light-receiving surface of the light-receiving unit 78b in the detection recess 78d, the influence of ambient light can be reduced. Furthermore, by arranging the illumination direction of the illumination unit 78a and the light-receiving direction of the light-receiving unit 78b so that they intersect, it is possible to prevent the relatively strong light from the illumination unit 78a from directly entering the light-receiving unit 78b. Note that an imaging element such as a CCD may be provided instead of the illumination unit 78a and the light-receiving unit 78b, and the position of a specific component layer may be identified based on the difference in color of each component layer.

[0030] 5, the light detection unit 70 has a storage rack 72 that stores tubes T, and a rack movement mechanism 72a that moves the storage rack 72 in a horizontal direction. The rack movement mechanism 72a preferably includes a first movement mechanism 72a1 that moves the sample container rack 72 in a first horizontal direction, and a second movement mechanism 72a2 that moves the sample container rack 72 and the first movement mechanism 72a1 in a second horizontal direction. The first and second movement mechanisms may be, for example, a belt drive mechanism including a belt and a drive motor for the belt, or an actuator.

[0031] The centrifugal separation apparatus 1 will be described using the block diagram of Figure 6. The control unit 80 (CPU) of the centrifugal separation apparatus 1 controls the centrifuge motor 23 that rotates the centrifuge unit 20, the arm drive motor 32 that drives the arm 31 of the pickup unit 30, the first suction and discharge mechanism 38 that is provided in the centrifugation dispensing unit 35 and performs suction and discharge from the first dispensing nozzle 36, and the first lift motor 41 that is provided in the second support unit 40 and raises and lowers the pickup unit 30 and the centrifugation dispensing unit 35.

[0032] The control unit 80 also controls a second suction and discharge mechanism 55 provided in the layer detection dispensing unit 51 and configured to perform suction and discharge using the multiple second dispensing nozzles 51a, a second lifting mechanism 58 provided in the second support unit 50 and configured to raise and lower the multiple second dispensing nozzles 51a, a first support unit moving motor 64 provided in the main support unit 60 and configured to move the first support unit 40 in the y direction, a second support unit moving motor 65 provided in the main support unit 60 and configured to move the second support unit 50 in the y direction, and a main support unit moving mechanism 63 provided in the main body 10 or the main body wall unit 13 and configured to move the main support unit 60 in the x direction.

[0033] Furthermore, the control unit 80 controls the illumination unit 78a, the light receiving unit 78b, the lifting mechanism 78c, and the rack moving mechanism 72a, which are related to the light detection unit 78 of the light detection unit 70. The control unit 80 receives the current value detected by the current detection unit 57 connected to the plus terminal unit 56a and the minus terminal unit 56b. The control unit 80 detects the characteristic layer based on the change in the received current value.

[0034] The operation of the centrifugal separation device 1 will now be described. In a first step, a tube T containing whole blood is placed in the centrifugal separation unit 20 by a user. In a second step, as shown in FIG. 7 , the control unit 80 moves the first dispensing nozzle 36 equipped with a dispensing tip to aspirate various reagents and solutions required for centrifugation of the whole blood from the bottle 16 and dispenses the various reagents and solutions into the tube 10 placed in the centrifugal separation unit 20. In a third step, the control unit 80 rotates the centrifugal separation unit 20 to perform centrifugation.

[0035] In a fourth step, as shown in Fig. 8, the control unit 80 causes the pair of arms 31 of the pickup unit 30 to grip the centrifuged tube T and move the gripped tube T upward to pull it out of the centrifugal separation unit 20. The control unit 80 then moves the tube T pulled out of the centrifugal separation unit 20 to the light detection unit 70. The movement of the tube T by the pickup unit 30 is preferably performed at a speed that allows the interface of the centrifugal separation layer in the tube T to be kept horizontal so as not to disturb the interface of the centrifugal separation layer.

[0036] In a fifth step, the control unit 80 uses the current detection unit 57 and / or the light detection unit 78 to detect a specific layer for each of the plurality of tubes T. The control unit 80 then dispenses the detected specific layer from each of the plurality of tubes T using the plurality of dispensing nozzles 51 of the layer detection dispensing unit.

[0037] Second Embodiment A centrifugal sorting apparatus 1A according to a second embodiment will be described with reference to Figures 10 to 13. Description of the centrifugal sorting apparatus 1A that is the same as that of the centrifugal sorting apparatus 1 according to the first embodiment will be omitted. The centrifugal sorting apparatus 1A includes two consumable storage units 12 and an extractor 90 that extracts a target substance (e.g., transferrin) from a separated specific layer (e.g., a buffy coat layer). The controller 80 uses a plurality of second dispensing nozzles 51a to dispense the specific layer separated from the tube T of the light detector 70 into the extractor 90.

[0038] The extraction device 90 will be described with reference to FIGS. 12 and 13 . The extraction device 90 includes a rocking body 90S supported on a base (housing) 91 so as to be rockable about a rocking shaft 96. The rocking body 90S includes a rocking mechanism 92 that rocks the rocking shaft 96. The rocking mechanism 92 preferably includes a belt 92a, multiple pulleys 92b, and a drive motor 92c. The rocking body 90S includes a cover 93, a temperature adjustment unit (e.g., a Peltier element) that supports multiple extraction containers 95 so as to be temperature-adjustable, and a magnet unit 98 that moves relative to the multiple extraction containers 95. The multiple extraction containers 95 are arranged in a line along the y direction. The temperature adjustment unit can preferably be integrated with the magnet unit 98. The control unit 80 controls the rocking mechanism 92, the temperature adjustment unit, and the movement mechanism of the magnet unit 98.

[0039] As shown in Fig. 13, the oscillating body 90S can be in (a) a horizontal state, (b) a first tilted state (a state rotated counterclockwise), and (c) a second tilted state (a state rotated clockwise). When the oscillating mechanism 92 operates, the oscillating body 90S rotates from the state (a) to the state (b) in Fig. 13 and from the state (a) to the state (c) in Fig. 13. The conditions and operation for extracting transferrin from the buffy coat layer using the extractor 90 are described in International Publication No. WO 2023 / 008270 by the present applicant.

[0040] In the multiple extraction devices 90, lectin-immobilized magnetic beads, to which lectin that binds to the sugar chains of transferrin is immobilized, are dispensed from the rack 14 into multiple extraction containers 95 using multiple dispensing nozzles 51a equipped with multiple dispensing tips. When the oscillating body 90S oscillates under the extraction conditions and with the above-described operation, the transferrin binds to the lectin-immobilized magnetic beads. When a movable magnet provided on the lower surface of the multiple extraction containers 95 is brought close to the lower surfaces of the multiple extraction containers 95 while the transferrin is bound to the lectin-immobilized magnetic beads, the lectin-immobilized magnetic beads bound to the transferrin are attracted by the magnetic force of the magnetic units 98 on the lower surfaces of the multiple extraction containers 95, allowing them to be separated.

[0041] REFERENCE SIGNS LIST 1, 1A Centrifugal separation device 10 Main body 20 Centrifugal separation unit 30 Pickup unit 35 Centrifugal separation dispensing unit 40 First support unit 50 Second support unit 51 Layer detection dispensing unit 57 Current detection unit 60 Main support unit 70 Light detection unit 80 Control unit 90 Extraction device

Claims

1. A centrifugation separation device for separating a specific layer from a plurality of centrifugation layers, comprising: a centrifugation section that accommodates a centrifugation container for containing a specimen solution and centrifugally separates the specimen solution; a pickup section that picks up the centrifugation container having the plurality of centrifugation layers produced by centrifugation of the specimen solution; a layer detection section that detects the specific layer from the plurality of centrifugation layers in the centrifugation container; a layer detection dispensing section that separates the specific layer; and a control section that controls the operation of each section.

2. A centrifugal separation device as described in claim 1, comprising a first support part supporting the pickup part, a second support part supporting the layer detection dispensing part, and a main support part supporting the first support part and the second support part.

3. A centrifugal separation device as described in claim 2, comprising: a first lifting mechanism for raising and lowering the first support part; a second lifting mechanism for raising and lowering the second support part; a first support part lateral movement mechanism for moving the first support part in a first lateral direction; and a second support part lateral movement mechanism for moving the second support part in the first lateral direction.

4. A centrifugal separation device according to claim 2, comprising a body or a body wall portion supporting said main support portion.

5. A centrifugal separation device according to claim 4, comprising a main support part movement mechanism for moving the main support part in a second lateral direction different from the first lateral direction.

6. A centrifugal separation device as described in claim 3, wherein the first support portion is provided on a first side surface of the main support portion, and the second support portion is provided on a second side surface of the main support portion opposite to the first side surface.

7. A centrifugal separation device according to claim 2, wherein the first support portion, the second support portion, and the main support portion are provided above a stage of the main body.

8. A centrifugal separation device as described in claim 1, wherein the layer detection unit is a current detection unit that detects the specific layer based on the electrical conductivity of the multiple centrifugation layers, and / or a light detection unit that detects the specific layer based on the transmitted light of the multiple centrifugation layers.

9. A centrifugal separation apparatus according to claim 8, wherein the current detection unit detects a current between a pair of electrodes provided in the layer detection dispensing unit.

10. A centrifugation separation device as described in claim 8, wherein the optical detection unit includes a storage rack that stores a plurality of centrifugation containers that have been centrifuged, arranged in a row in the first horizontal direction, and the layer detection dispensing unit includes a plurality of dispensing nozzles arranged in a row in the first horizontal direction.

11. A centrifugal separation apparatus according to claim 1, comprising an extraction device for extracting a target substance from the specific layer separated from the layer detection dispensing section.

12. A centrifugal separation apparatus according to claim 11, wherein the extraction device comprises a plurality of rocking containers arranged in a row in the first horizontal direction, and a rocking mechanism for rocking the plurality of rocking containers.

13. A centrifugal separation apparatus according to claim 1, further comprising a centrifugation dispensing section for dispensing various reagents and / or solutions into said centrifugation container.

14. A centrifugal separation apparatus according to claim 13, comprising a consumables storage section for storing consumables including the various reagents and / or solutions.

15. A centrifugal separation device as described in claim 14, wherein the consumable storage section stores the containers for the various reagents, the containers for the solutions, and / or the dispensing tips to be attached to the multiple dispensing nozzles, arranged in a row in the first horizontal direction.