Carbon-capturing soil conditioner and preparation method therefor

Abstract

The present invention relates to a carbon-capturing soil conditioner and a preparation method therefor, and the objective of the present invention is to provide a carbon-capturing soil conditioner and a preparation method therefor, the carbon-capturing soil conditioner adsorbing carbon dioxide from the atmosphere and enabling soil to be provided even in a place where soil did not previously exist. The carbon-capturing soil conditioner according to the present invention comprises: a porous material on which bacteria with carbon dioxide absorption mechanism are adsorbed; a first coating layer which includes a natural soil including clay and / or loess fine powder and a coagulant, and which is applied to the surface of the porous material; and a second coating layer formed of a coating agent including a silicate-based accelerator, and applied to the surface of the first coating layer.

Description

Carbon capture soil conditioner and method for manufacturing the same

[0001] The present invention relates to a soil conditioner and a method for producing the same, and more particularly, to a carbon capture soil conditioner that absorbs carbon dioxide in the atmosphere, including bacteria having a carbon dioxide absorption mechanism, and a method for producing the same.

[0002] As the damage caused by climate change increases, interest in reducing carbon dioxide emissions, a key driver of climate change, is growing. Consequently, various efforts are being made across various industries to reduce carbon dioxide emissions. In particular, cement, essential for the production of concrete in the construction industry, is known to emit over 0.8 tons of carbon dioxide per ton produced. Therefore, the construction industry is striving to achieve carbon neutrality, reducing net carbon dioxide emissions to zero.

[0003] Soils can generally store more than twice the amount of carbon dioxide in the atmosphere, acting as a buffer to regulate atmospheric carbon dioxide levels. However, soils suitable for carbon dioxide storage are those rich in sand or gravel to facilitate rapid carbon dioxide dissipation and have a low water table. However, securing sufficient soils suitable for carbon dioxide storage in nature presents a challenge.

[0004] Among related prior art technologies, Patent Publication No. 10-1313091 discloses a soil methane gas reduction composition that utilizes alkaline earth metals and zeolites substituted with alkaline ions to reduce methane, a greenhouse gas emitted during rice cultivation. While this prior art can reduce methane emitted from soil, it is difficult to expect a carbon dioxide reduction effect. Furthermore, since this prior art requires spraying the methane reduction composition onto existing soil, it is difficult to utilize in cases where securing sufficient soil is difficult.

[0005] [Patent Document] Patent Publication No. 10-1313091 (September 30, 2013)

[0006] Accordingly, the purpose of the present invention is to provide a carbon capture soil conditioner and a method for manufacturing the same, which can absorb carbon dioxide in the atmosphere and provide soil where no soil exists.

[0007] In order to achieve the above object, a carbon capture soil conditioner according to the present invention comprises: a porous material to which bacteria having a carbon dioxide absorption mechanism are adsorbed; a natural soil including at least one of clay and yellow soil fine powder, and a coagulant, the first coating layer coating the surface of the porous material; and a second coating layer formed of a coating agent including a silicate-based accelerator, the second coating layer coating the surface of the first coating layer.

[0008] It may include 60 to 75 wt% of the above natural soil, 5 to 25 wt% of the above porous material, 5 to 10 wt% of the above coagulant, and 1 to 7 wt% of the above coating agent.

[0009] The porous material may include at least one of expanded quartz and zeolite.

[0010] The above expanded stone may have a diameter of 1.2 mm or less.

[0011] The above zeolite may have a diameter of 2.5 to 3.5 μm.

[0012] The above natural soil may have a particle size of 0.005 to 0.02 mm.

[0013] The above bacteria may be at least one species selected from the group consisting of Rhodobacter capsulatus, Rhodopseudomonas palustris, Rhodobacter blasticus, and Rhodobacter sphaeroids.

[0014] The above bacteria may be at least one species selected from the group consisting of Thermoproteus, Sulfolobus, Planctomyces, and Anammox.

[0015] The coagulant may include at least one of honey, corn syrup, and syrup.

[0016] A method for manufacturing a carbon capture soil conditioner according to the present invention may include the steps of: preparing a first coating layer composition by mixing natural soil containing at least one of clay and yellow clay fine powder and a coagulant; introducing the first coating layer composition and a porous material adsorbed with bacteria having a carbon dioxide adsorption mechanism into a ventilator to form a first coating layer on the surface of the porous material; and forming a second coating layer by spraying a coating agent on the surface of the porous material on which the first coating layer has been formed.

[0017] The soil conditioner according to the present invention can adsorb carbon dioxide in the atmosphere using bacteria that adsorb carbon dioxide insensitive to anaerobic, aerobic, and light / dark conditions.

[0018] The soil conditioner according to the present invention can create an environment in which bacteria can grow even within the soil conditioner by using a porous material to which bacteria are adsorbed.

[0019] When the soil conditioner of the present invention is mixed and used in natural soil, the coating layer melts upon contact with external moisture and natural soil, exposing the porous material to the outside, and bacteria adsorbed to the porous material can seep into the natural soil, continuously reproduce, and adsorb carbon dioxide in the atmosphere.

[0020] Figure 1 is a drawing showing a soil improvement agent according to the present invention.

[0021] Figure 2 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the Calvin cycle.

[0022] Figure 3 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the reverse TCA cycle.

[0023] Figure 4 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the hydroxypropionate cycle.

[0024] Figure 5 is a flow chart showing a method for manufacturing a soil improvement agent according to the present invention.

[0025] Figure 6 is a graph showing the carbon dioxide adsorption performance according to the type of bacteria according to an experimental example.

[0026] Figure 7 is a graph showing the carbon dioxide adsorption performance according to the type of porous material according to an experimental example.

[0027] Figure 8 is a graph showing the number of surviving bacteria according to the type of porous material according to an experimental example.

[0028] Figure 9 is a graph showing the carbon dioxide adsorption performance of soil improvers according to comparative examples and examples.

[0029] Figure 10 is a photograph showing the number of surviving bacteria in a soil conditioner according to an embodiment.

[0030] The present invention is being filed with the support of the tasks described in [Table 1] and [Table 2].

[0031] Project ID: 1415187362 (Agency) Project Details: 20025773 Ministry: Ministry of Trade, Industry and Energy Research Management Agency: Korea Institute of Industrial Technology Planning and Evaluation Research Project Name: Industrial Technology Alchemist Project Research Project Name: Development of Building-Integrated Carbon Dioxide Capture and Conversion Technology Host Agency: Kyungpook National University Industry-Academic Cooperation Group Research Period: April 1, 2023 - December 31, 2024

[0032] Project Unique Number 2400426363 (Agency) Detailed Project Number RS-2024-00426363 Ministry Name Ministry of Science and ICT Research Management Specialized Institution Science and Technology Commercialization Promotion Agency Research Project Name Industry-Academia-Research Cooperation Activation Support (R&D) / University Technology Management Promotion Research Project Name IP Advancement and Practicalization for Commercialization of Eco-Friendly Concrete Technology Using Bacteria for Carbon Removal Supervising Institution Kyunghee University Industry-Academia Cooperation Group Research Period 2024.04.01 - 2025.12.31

[0033] It should be noted that in the following description, only the parts necessary for understanding the embodiments of the present invention are described, and the description of other parts will be omitted to the extent that it does not deviate from the gist of the present invention.

[0034] The terms and words used in this specification and claims described below should not be interpreted as limited to their conventional or dictionary meanings, but should be interpreted with meanings and concepts that conform to the technical idea of ​​the present invention based on the principle that the inventor can appropriately define the concept of the term to best describe his or her own invention. Therefore, the embodiments described in this specification and the configurations illustrated in the drawings are merely preferred embodiments of the present invention and do not represent all of the technical idea of ​​the present invention. Therefore, it should be understood that there may be various equivalents and modified examples that can replace them at the time of this application.

[0035] Hereinafter, embodiments of the present invention will be described in more detail with reference to the attached drawings.

[0036] Figure 1 is a drawing showing a soil improvement agent according to the present invention.

[0037] A soil conditioner (100) according to the present invention comprises a porous material (10) to which bacteria having a carbon dioxide adsorption mechanism are adsorbed, natural soil including at least one of clay and yellow soil fine powder, and a coagulant, a first coating layer (20) that coats the surface of the porous material (10), and a second coating layer (30) formed of a coating agent including a silicate-based accelerator and coating the surface of the first coating layer (20).

[0038] Hereinafter, the composition of the soil improvement agent (100) according to the present invention will be described in more detail.

[0039] The bacteria according to the present invention may have a carbon dioxide absorption mechanism. In particular, it is preferred that the bacteria according to the present invention be insensitive to anaerobic, aerobic, and light / dark conditions. Bacteria with these characteristics can be classified into bacteria that utilize the Calvin Cycle, the Reverse Tricarboxylic Acid Cycle, and the Hydroxypropionate Cycle as their carbon dioxide absorption mechanisms, depending on their carbon dioxide absorption mechanism.

[0040] First, the bacteria having the Calvin cycle as a carbon dioxide absorption mechanism may include at least one species selected from the group consisting of Rhodobacter capsulatus, Rhodopseudomonas palustris, Rhodobacter blasticus, and Rhodobacter sphaeroids, which are found in purple non-sulfur photosynthetic bacteria.

[0041] Secondly, the bacteria having a reverse TCA cycle carbon dioxide absorption mechanism may include one or more species selected from the group consisting of Thermoproteus and Sulfolobus.

[0042] Thirdly, the bacteria having the hydroxypropionate cycle as a carbon dioxide absorption mechanism may be green non-sulfur bacteria, and may preferably include at least one species selected from the group consisting of Planctomyces and Anammox.

[0043] Hereinafter, the carbon dioxide adsorption mechanism of bacteria according to the present invention will be described in more detail.

[0044] Figure 2 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the Calvin cycle.

[0045] Referring to Figure 2, the Calvin cycle absorbs six molecules of carbon dioxide during the process of synthesizing glucose from ATP and NADPH. The Calvin cycle can be broadly divided into three stages. The first stage is the carbon dioxide fixation stage, which is catalyzed by an enzyme called rubisco. Rubisco combines carbon dioxide with ribulose 1,5-bisphosphate (RuBP), which consists of five carbon atoms, to produce two molecules of glycerol triphosphate (3PG). The second stage is the process in which glycerol triphosphate (3PG) undergoes a reduction process and is converted to glyceraldehyde triphosphate (G3P). The glyceraldehyde triphosphate (G3P) thus produced is used to synthesize glucose. The third and final stage is the reaction in which ribulose 1,5-bisphosphate (RuBP) is regenerated. In the second step, the glyceraldehyde 3-phosphate (G3P) produced is converted to ribulose monophosphate (RuMP) and then to ribulose 1,5-bisphosphate (RuBP). This regenerated ribulose 1,5-bisphosphate (RuBP) can then repeat the process of combining with carbon dioxide and adsorb carbon dioxide.

[0046] Figure 3 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the reverse TCA cycle.

[0047] Referring to Figure 3, the reverse TCA cycle is the TCA cycle that runs in reverse, absorbing three molecules of carbon dioxide in the process of converting carbon dioxide into acetyl CoA. Enzymes used in the reverse TCA cycle include fumarate reductase, which reduces fumarate to succinate; α-ketoglutarate:ferredoxin oxidoreductase, which transfers electrons received from ferrodoxin to carbon dioxide and succinyl CoA to reduce it to α-ketoglutarate; and ATP citrate lyase, which breaks down citrate to create oxaloacetate and acetyl CoA.

[0048] Figure 4 is a diagram showing the mechanism by which bacteria according to the present invention absorb carbon dioxide through the hydroxypropionate cycle.

[0049] Referring to Figure 4, the hydroxypropionate cycle absorbs two molecules of carbon dioxide during the process of converting acetyl CoA to malyl CoA. Specifically, looking at the steps of the hydroxypropionate cycle to absorb carbon dioxide, acetyl CoA first absorbs carbon dioxide to form malonyl CoA, and malonyl CoA is converted to propionyl CoA via the intermediate hydroxypropionate. Next, propionyl CoA absorbs carbon dioxide during the process of carboxylation to methylmalonyl CoA, and methylmalonyl CoA isomerizes to succinyl CoA. Afterwards, succinyl CoA forms malyl CoA, and malyl CoA is decomposed into acetyl CoA and glyoxylate.

[0050] The 3-hydroxypropionate / 4-hydroxybutyrate cycle and the dicarboxylate / 4-hydroxybutyrate cycle are known as modified carbon dioxide absorption mechanisms of the hydroxypropionate cycle.

[0051] The soil conditioner (100) according to the present invention can adsorb carbon dioxide in the atmosphere by using bacteria that adsorb carbon dioxide insensitive to anaerobic, aerobic, and light / dark conditions.

[0052] When simply adding bacteria during the manufacturing of a soil conditioner (100), the bacteria are likely to die due to friction and impact during the mixing process of the soil conditioner (100). Therefore, the soil conditioner (100) according to the present invention can create an environment in which bacteria can grow even within the soil conditioner (100) by using a porous material (10) to which bacteria are adsorbed.

[0053] The porous material (10) may include at least one of expanded vermiculite and zeolite having excellent cation exchange capacity. For example, the porous material (10) may include at least one of expanded vermiculite having a diameter of 1.2 mm or less and zeolite having a diameter of 2.5 to 3.5 μm.

[0054] The porous material (10) preferably has an effective moisture content of 40 vol% or more and a porosity of 50 vol% or more, thereby creating an environment in which bacteria can grow. In particular, the porous material (10) has an exchangeable cation (Mg) present on the surface. 2+ , Ca 2+ ) has the property of absorbing organic matter, so it can absorb bacteria and the culture solution necessary for bacterial growth.

[0055] The first coating layer (20) may include natural soil and a coagulant. Here, the natural soil may include at least one of clay and yellow soil powder. If the natural soil has a particle size of less than 0.005 mm, the natural soil may block the pores of the porous material (10) and inhibit the growth of bacteria adsorbed to the porous material (10). If the particle size exceeds 0.02 mm, coagulation may not occur by the coagulant. Therefore, it is preferable that the natural soil have a particle size of 0.005 to 0.02 mm.

[0056] The coagulant can coagulate natural soil, thereby coating the natural soil with a porous material (10). The coagulant can include at least one of honey, corn syrup, and grain syrup. This coagulant not only coagulates and coats the natural soil, but also acts as a nutrient for bacteria, thereby providing an environment in which bacteria can initially grow stably within the soil conditioner (100).

[0057] The first coating layer (20) can be coated on the surface of the porous material (10) to form a ring shape. However, since the first coating layer (20) can easily fall off from the porous material (10), the ring shape can easily collapse. Therefore, the soil conditioner (100) according to the present invention can temporarily maintain the ring shape of the soil conditioner (100) by forming a second coating layer (30) that coats the surface of the first coating layer (20).

[0058] The second coating layer (30) can coat the surface of the first coating layer (20) coated on the surface of the porous material (10). The second coating layer (30) can be formed of a coating agent including a silicate-based accelerator having sodium silicate hydrate (Na2SiO3·nH2O) as a main component, but is not limited thereto.

[0059] The soil conditioner (100) according to the present invention can control the amount of carbon that can be adsorbed by bacteria adsorbed on the porous material (10) depending on the thickness of the first and second coating layers (20, 30). For example, the soil conditioner (100) according to the present invention can include 60 to 75 wt% of natural soil, 5 to 25 wt% of porous material (10), 5 to 10 wt% of coagulant, and 1 to 7 wt% of coating agent.

[0060] The soil conditioner (100) according to the present invention can be used by mixing it with existing natural soil or as a substitute for artificial lightweight soil.

[0061] In particular, the soil conditioner (100) according to the present invention is formed in a ring shape and can initially maintain its shape by the second coating layer (30), but the ring shape may collapse over time and the first coating layer (20) may be exposed to the outside. Thereafter, the first coating layer (20) may be peeled off and the porous material (10) may be exposed.

[0062] Therefore, when the soil conditioner (100) of the present invention is mixed and used in natural soil, the coating layer (20, 30) melts due to contact with external moisture and natural soil, and the porous material (10) is exposed to the outside, and bacteria adsorbed to the porous material (10) can seep into the natural soil, continuously reproduce, and adsorb carbon dioxide in the atmosphere.

[0063] Hereinafter, the method for manufacturing a soil improvement agent according to the present invention will be described in more detail.

[0064] Figure 5 is a flow chart showing a method for manufacturing a soil improvement agent according to the present invention.

[0065] Referring to FIG. 5, first, in step S10, a first coating layer composition is prepared by mixing natural soil and a coagulant including at least one of clay and yellow soil powder.

[0066] Here, the natural soil may have a particle size of 0.005 to 0.02 mm, and the coagulant may include at least one of honey, corn syrup, and grain syrup.

[0067] Next, in step S20, the first coating layer composition and the porous material on which bacteria having a carbon dioxide adsorption mechanism are adsorbed are introduced into a ventilator to form a first coating layer on the surface of the porous material.

[0068] Here, the porous material may include at least one of expanded vermiculite having a diameter of 1.2 mm or less and zeolite having a diameter of 2.5 to 3.5 μm.

[0069] The bacteria may be at least one species selected from the group consisting of Rhodobacter capsulatus, Rhodopseudomonas palustris, Rhodobacter blasticus, and Rhodobacter sphaeroids. Furthermore, the bacteria may be at least one species selected from the group consisting of Thermoproteus, Sulfolobus, Planctomyces, and Anammox.

[0070] The porous material can be sterilized using nanobubble water, then completely immersed in a culture medium containing bacteria at a weight ratio of 1:3 to 4, and subjected to negative pressure to adsorb the bacteria. The process of applying negative pressure can be performed at 10 to 30 torr for 20 to 40 minutes within a negative pressure container, but is not limited thereto.

[0071] Nanobubble water, which sterilizes porous materials, is comprised of ultra-fine, oxygen-laden water bubbles, each containing a large amount of oxygen. Therefore, cleaning porous materials with nanobubble water can increase the amount of dissolved oxygen within the porous material, thereby enhancing the activity of photosynthetic bacteria. Furthermore, nanobubble water can remove foreign substances attached to the surface of porous materials. Furthermore, because the hydroxyl radicals generated in water have a negative charge, they can adsorb (+) ions, which are pollutants, and remove them. During the formation of nanobubbles in nanobubble water, oxygen in the air is activated to form molecules such as ozone (O3) and hydroxyl groups (-OH), which act as sterilizing agents that destroy organic matter and volatile organic compounds. In this case, the porous material can be washed with nanobubble water and then dried at 100°C for 6 to 10 hours, but is not limited thereto.

[0072] In order for the soil improvement composition according to the present invention to have a desirable carbon dioxide adsorption rate, bacteria are grown at 1x10 in an incubator at 5 to 50°C. 9 It can be cultured in a culture medium at a concentration of 100 cells / mL or more. Here, the culture medium may include, but is not limited to, yeast extract, disodium succinate hexahydrate, and potassium dihydrogen phosphate (KH2PO4). For example, the culture medium may include 1.0 g / L of yeast extract, 1.0 g / L of disodium succinate hexahydrate, 0.5 mL / L of ethanol, 1 mL / L of ferric citrate solution (0.5%), 0.5 g / L of potassium dihydrogen phosphate (KH2PO4), and magnesium sulfate (MgSO) for 1 L of purified water. 4· 7H2O) 0.4g / L, sodium chloride (NaCl) 0.4g / L, ammonium chloride (NH4Cl) 0.4g / L, calcium chloride (CaCl 2·2H2O) 0.05 g / L, and trace element solution (Trace element solution SL-6) 1 mL / L.

[0073] The porous material on which bacteria are adsorbed can be placed in a rotary ventilator together with the first coating layer composition to form a first coating layer on the surface. At this time, the ventilator can be rotated at a speed of 20 to 40 rpm for 30 seconds to 2 minutes to form the first coating layer on the surface of the porous material.

[0074] Finally, in step S30, a coating agent including a silicate-based accelerator is sprayed onto the surface of the porous material on which the first coating layer has been formed to form a second coating layer.

[0075] At this time, the second coating layer can be formed by spraying a coating agent onto the surface of the porous material on which the first coating layer is formed using a compressed sprayer.

[0076]

[0077] [Experimental Example]

[0078] Evaluation of carbon dioxide adsorption performance according to bacterial type

[0079] Hereinafter, in order to evaluate the carbon dioxide adsorption performance according to the type of bacteria according to the present invention, media according to the first to fifth experimental examples were prepared.

[0080] The first experimental example is a basic medium in which no bacteria are cultured, the medium according to the second experimental example cultured Rhodobacter capsulatus, the medium according to the third experimental example cultured Rhodopseudomonas palustris, the medium according to the fourth experimental example cultured Rhodobacter blasticus, and the medium according to the fifth experimental example cultured Rhodobacter sphaeroids.

[0081] The media according to Experimental Examples 1 to 5 were placed in sealed containers, and carbon dioxide was added to the containers to achieve carbon dioxide saturation. After 24 hours, the amount of carbon dioxide captured by the bacteria cultured in each medium was measured by measuring the decrease in carbon dioxide compared to the initial level.

[0082] Figure 6 is a graph showing the carbon dioxide adsorption performance according to the type of bacteria according to an experimental example.

[0083] Referring to Fig. 6, it can be seen that in the first experimental example, no carbon dioxide was captured, whereas in the second experimental example, 2,400 ppm, in the third experimental example, 6,800 ppm, in the fourth experimental example, 2,800 ppm, and in the fifth experimental example, 2,000 ppm of carbon dioxide were captured. In other words, it can be seen that Rhodopseudomonas palustris has the best carbon dioxide adsorption performance.

[0084]

[0085] Evaluation of carbon dioxide adsorption performance according to the type of porous material

[0086] Hereinafter, in order to evaluate the carbon dioxide adsorption performance according to the type of porous material according to the present invention, porous materials according to the 6th to 15th experimental examples were manufactured.

[0087] The porous material according to the sixth experimental example is expanded vermiculite, the porous material according to the seventh experimental example is expanded vermiculite having Rhodobacter capsulatus adsorbed thereon, the porous material according to the eighth experimental example is expanded vermiculite having Dopseudomonas palustris adsorbed thereon, the porous material according to the ninth experimental example is expanded vermiculite having Rhodobacter blasticus adsorbed thereon, and the porous material according to the tenth experimental example is expanded vermiculite having Rhodobacter spheroides adsorbed thereon.

[0088] The porous material according to the 11th experimental example is a zeolite, the porous material according to the 12th experimental example is a zeolite having Rhodobacter capsulatus adsorbed on it, the porous material according to the 13th experimental example is a zeolite having Dopseudomonas palustris adsorbed on it, the porous material according to the 14th experimental example is a zeolite having Rhodobacter blasticus adsorbed on it, and the porous material according to the 15th experimental example is a zeolite having Rhodobacter spheroides adsorbed on it.

[0089] The porous materials according to Experimental Examples 6 to 15 were placed in a sealed container, and carbon dioxide was added to the container to achieve carbon dioxide saturation. After 24 hours, the amount of carbon dioxide captured by each porous material was measured by measuring the decrease in carbon dioxide compared to the initial level.

[0090] Figure 7 is a graph showing the carbon dioxide adsorption performance according to the type of porous material according to an experimental example.

[0091] Referring to Fig. 7, it can be confirmed that the porous material according to Experimental Example 6 captures 800 ppm of carbon dioxide, and the porous material according to Experimental Example 11 captures 3,200 ppm of carbon dioxide. That is, it can be confirmed that the carbon dioxide adsorption performance of zeolite is superior to that of expanded vermiculite. However, it can be confirmed that the porous materials according to Experimental Examples 7 to 10 capture 2,000 to 6,800 ppm of carbon dioxide, and the porous materials according to Experimental Examples 12 to 15 capture 1,200 to 4,000 ppm of carbon dioxide. That is, it can be confirmed that the carbon dioxide adsorption performance of expanded vermiculite with bacteria adsorbed is superior to that of zeolite with bacteria adsorbed. In particular, it can be confirmed that the carbon dioxide adsorption performance of expanded vermiculite with Rhodopseudomonas palustris adsorbed is the best.

[0092]

[0093] Evaluation of bacterial growth potential according to the type of porous material

[0094] In order to evaluate the growth potential of bacteria according to the type of porous material according to the present invention, the number of surviving bacteria present in the porous material according to the 6th to 15th experimental examples was measured.

[0095] 1 g of the porous material according to Experimental Examples 6 to 15 was collected and added to 50 ml of inorganic medium, and bacteria were removed from the porous material in a stirrer at 30°C for 3 hours. Afterwards, the culture medium of the removed bacteria was added to 10 -6 100 mg of the diluted sample was inoculated onto an agar medium and cultured for 3 days. Based on the counting results of the bacterial colonies formed through re-cultivation on the agar medium, the number of surviving bacteria present in the porous materials according to Experimental Examples 6 to 15 was measured.

[0096] Figure 8 is a graph showing the number of surviving bacteria according to the type of porous material according to an experimental example.

[0097] Referring to Fig. 8, the number of surviving bacteria present in the porous materials according to the 7th to 10th experimental examples and the 12th to 15th experimental examples is 7.23 × 10 7 ~ 3.18 × 10 8 You can see that it has a range.

[0098]

[0099] [Comparative Examples and Examples]

[0100] Hereinafter, in order to confirm the characteristics of the soil improver according to the present invention, soil improvers according to comparative examples and examples were manufactured.

[0101] According to the first comparative example, a soil conditioner was manufactured using only clay, and according to the second comparative example, a soil conditioner was manufactured by coating a first coating layer mixed with clay and honey on the surface of expanded vermiculite, and according to the example, a soil conditioner was manufactured by coating a first coating layer mixed with clay and honey on the surface of expanded vermiculite to which Rhodopseudomonas palustris was adsorbed.

[0102] Comparative Examples 1 and 2, the porous materials according to the examples were placed in sealed containers, and carbon dioxide was added to the containers to achieve carbon dioxide saturation. After 24 hours, the amount of carbon dioxide captured by each soil conditioner was measured by measuring the decrease in carbon dioxide compared to the initial level.

[0103] Figure 9 is a graph showing the carbon dioxide adsorption performance of soil improvers according to comparative examples and examples.

[0104] Referring to FIG. 9, it can be confirmed that the soil conditioner according to the first comparative example did not capture carbon dioxide, and the soil conditioner according to the second comparative example captured 3,400 ppm of carbon dioxide. On the other hand, it can be confirmed that the soil conditioner according to the example captured 9,000 ppm of carbon dioxide. Although the amount of carbon dioxide captured by the soil conditioner according to the example varies depending on the thickness of the coating layer, it can be confirmed that it captures 4,800 to 9,000 ppm of carbon dioxide. In other words, it can be confirmed that the soil conditioner including the porous material to which bacteria are adsorbed has excellent carbon dioxide adsorption performance.

[0105] Figure 10 is a photograph showing the number of surviving bacteria in a soil conditioner according to an embodiment.

[0106] As shown in Fig. 10, the number of surviving bacteria present in the soil conditioner according to the embodiment was measured to be 4.5±0.42 × 10 4 It was confirmed that bacteria at cell / mL survived.

[0107] Meanwhile, the embodiments disclosed in this specification and drawings are merely specific examples to aid understanding and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that other modifications based on the technical concepts of the present invention are possible in addition to the embodiments disclosed herein.

[0108] [Explanation of symbols]

[0109] 100: Soil conditioner

[0110] 10: Porous materials

[0111] 20: First coating layer

[0112] 30: Second coating layer

Claims

1. A porous material adsorbed with bacteria having a carbon dioxide adsorption mechanism; A first coating layer comprising natural soil including at least one of clay and yellow clay powder, and a coagulant, coating the surface of the porous material; and A second coating layer formed of a coating agent including a silicate-based rapid stabilizer and coating the surface of the first coating layer; A carbon-capturing soil conditioner comprising:

2. In paragraph 1, A carbon capture soil improvement agent characterized by comprising 60 to 75 wt% of the natural soil, 5 to 25 wt% of the porous material, 5 to 10 wt% of the coagulant, and 1 to 7 wt% of the coating agent.

3. In paragraph 2, A carbon capturing soil improvement agent, characterized in that the porous material comprises at least one of expanded vermiculite and zeolite.

4. In paragraph 3, A carbon capturing soil improvement agent, characterized in that the above expanded vermiculite has a diameter of 1.2 mm or less.

5. In paragraph 3, The above zeolite is a carbon capturing soil improvement agent characterized by having a diameter of 2.5 to 3.5㎛.

6. In paragraph 2, The above natural soil is a carbon capture soil improvement agent characterized by a particle size of 0.005 to 0.02 mm.

7. In paragraph 2, A carbon capture soil improvement agent, characterized in that the bacteria is at least one selected from the group consisting of Rhodobacter capsulatus, Rhodopseudomonas palustris, Rhodobacter blasticus, and Rhodobacter sphaeroids.

8. In paragraph 2, A carbon capture soil improvement agent, characterized in that the bacteria is at least one species selected from the group consisting of Thermoproteus, Sulfolobus, Planctomyces, and Anammox.

9. In any one of paragraphs 1 to 8, A carbon capture soil improvement agent characterized in that the coagulant comprises at least one of honey, starch syrup, and brown rice syrup.

10. A step of preparing a first coating layer composition by mixing natural soil and a coagulant including at least one of clay and yellow clay powder; A step of forming a first coating layer on the surface of the porous material by introducing the first coating layer composition and the porous material having the carbon dioxide adsorption mechanism into a ventilator; and A step of forming a second coating layer by spraying a coating agent including a silicate-based accelerator onto the surface of a porous material on which the first coating layer is formed; A method for manufacturing a carbon capturing soil improvement agent comprising:

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