Method for culturing cordyceps having enhanced functional components using hemp seeds

Abstract

The present invention relates to: a method for cultivating Cordyceps, comprising a step of inoculating a starter culture of Cordyceps into a mixed medium of hemp seeds and brown rice and culturing same; Cordyceps grown by the method; and a method for increasing the cordycepin and adenosine contents of Cordyceps.

Description

Cultivation method of Cordyceps militaris with enhanced functional properties using hemp seeds

[0001] The present invention relates to a method for culturing Cordyceps militaris with enhanced functional components, characterized in that it comprises the steps of: preparing a hemp mixed medium by adding water to a mixture of soaked hemp seeds and brown rice, and then sterilizing the mixture; and the step of inoculating Cordyceps militaris spores into the hemp mixed medium and culturing them. The Cordyceps militaris cultured by the method is a very useful invention that can enhance the food value.

[0002] Cordyceps militaris is a small mushroom belonging to the Clavicipitaceae family of the Ascomycetes order of the Ergot fungi order. Its name comes from its appearance, which is that it lives inside insects in the winter and sprouts like grass in the summer. The mechanism by which Cordyceps militaris is formed is that the Cordyceps militaris fungus forms conidia or ascospores, attaches to the joints, digestive organs, etc. of living insects, and then consumes nutrients from the insect's body, forming hyphae, which eventually kill the insect. When winter passes and summer comes and the temperature and humidity rise, mushrooms emerge from the insect's buried body and grow above the ground. In this way, Cordyceps militaris refers to the combination of a dead insect filled with hyphae and the mushroom that grows from its body.

[0003] Cordyceps militaris is divided into three parts: the head, the stipe, and the host. The head contains the spores used for reproduction, and the long part that supports the head is the stipe. The head and stipe together are called the fruiting body or mushroom, and the tip of the stipe protrudes from the pupa body. The head is convex, spherical, cylindrical with pointed ends, or spatula-shaped, and multiple perithecium are formed on its surface or beneath the epidermis.

[0004] Cordyceps militaris is generally composed of water, fat, crude protein, carbohydrates, and ash, and the protein contains about 18 kinds of amino acids essential for the human body. In addition, Cordyceps militaris contains special ingredients such as cordyceps polysaccharide and cordyceic acid, which are known to be used in the treatment of anemia, fatigue, high blood pressure, pulmonary tuberculosis, asthma, etc. Because it parasitizes insects, it is also very useful as a biopesticide that provides effective pest control while preventing environmental pollution. Cordyceps militaris has been passed down in China since ancient times as the "elixir of longevity for emperors," and it is recorded in the Compendium of Materia Medica that "when used regularly, it has a clear effect of strengthening weak constitutions and enhancing immunity." The mycelia obtained during spawn culture are known to produce mannitol, which is used for medicinal purposes, as well as other useful substances. Additionally, the fruiting body contains a component called cordycepin, which is known as an isomer of quinic acid. Cordycepin acts as a toxin to host insects and is an active substance with antibacterial, antiviral, and anticancer effects, and is known to be excellent for various diseases related to aging. Despite these excellent effects, collecting Cordyceps militaris fruiting bodies grown in a natural state is very difficult, so artificial cultivation technology is required to ensure a stable supply of Cordyceps militaris fruiting bodies.

[0005] Korean Patent No. 2062275 discloses a method for producing Cordycepin-rich Cordyceps militaris with increased cordycepin content. The patent relates to a method for increasing cordycepin content by processing Cordyceps militaris through heat treatment, steaming, and drying. In addition, Korean Patent No. 2518525 discloses a culturing method for increasing cordycepin content of Cordyceps militaris mycelia. The patent relates to a method for increasing cordycepin content by inoculating Cordyceps militaris mycelia with a medium containing woody raw materials and culturing them while irradiating them with LED mixed light. However, there is no technology in the prior art for increasing functional components of Cordyceps militaris using hemp seeds like the present invention.

[0006] The present invention was derived from the above-mentioned needs, and the purpose of the present invention is to establish a method for cultivating Cordyceps militaris, which can produce Cordyceps militaris with improved palatability and increased content of cordycepin and adenosine, which are the key effective ingredients of Cordyceps militaris, while optimizing hemp seed pretreatment, medium preparation, and culture conditions to be suitable for Cordyceps militaris cultivation and to enhance functional ingredients.

[0007] In order to solve the above problem, the present invention provides a method for culturing Cordyceps militaris, characterized by comprising: (1) a step of soaking hemp seeds in water; (2) a step of adding water to a mixture of the soaked hemp seeds in step (1) and brown rice, and then sterilizing the mixture to prepare a hemp mixed medium; and (3) a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing the mixture.

[0008] In addition, the present invention provides Cordyceps militaris cultured by the above method.

[0009] In addition, the present invention provides a method for increasing the cordycepin and adenosine content of Cordyceps militaris, characterized by comprising: (1) a step of soaking hemp seeds in water; (2) a step of adding water to a mixture of the soaked hemp seeds in step (1) and brown rice, and then sterilizing the mixture to prepare a hemp mixed medium; and (3) a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing the mixture.

[0010] Cordyceps militaris cultured by the method of the present invention has increased cordycepin and adenosine contents, and it is judged that the present invention can be usefully used for cultivating high-quality Cordyceps militaris.

[0011] In addition, Cordyceps sinensis cultured using the method of the present invention is safe as THC (Tetrahydrocannabinol), a hallucinogenic component of cannabis, is not detected at all, and its taste and flavor are also superior.

[0012] Figure 1 shows the results of culturing Cordyceps militaris for 6 weeks using hemp medium with different amounts of water added to hemp seeds soaked for 48 hours.

[0013] Figure 2 shows the results of culturing Cordyceps militaris for 6 weeks using hemp medium with different amounts of water added to hemp seeds soaked for 24 hours.

[0014] Figure 3 shows the results of culturing Cordyceps militaris for 6 and 8 weeks in a mixed medium of hemp seeds and brown rice.

[0015] In order to achieve the above purpose, the present invention

[0016] (1) Step of soaking hemp seeds in water;

[0017] (2) A step of preparing a hemp mixed medium by adding water to a mixture of brown rice and the hemp seeds of step (1) above and then sterilizing it; and

[0018] (3) A method for culturing Cordyceps militaris is provided, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them.

[0019] In the method for cultivating Cordyceps militaris of the present invention, the hemp seed variety used is the IH3 variety, which has a lower THC (Tetrahydrocannabinol) content and a higher CBD (Cannabidiol) content than the conventional variety. In addition, when using the peeled hemp seed (Cannabis sativa seed), Cordyceps militaris hardly grows, so it is preferable to use the harvested hemp seed as is.

[0020] In addition, in the method for cultivating Cordyceps militaris of the present invention, the step (1) may preferably be performed by soaking 25 to 50 parts by weight of hemp seeds in water at 20 to 25°C for 20 to 28 hours, and more preferably by soaking 25 to 50 parts by weight of hemp seeds in water at 20 to 25°C for 24 hours. If the hemp seeds are used without soaking, the Cordyceps militaris growth rate decreases, so it is preferable to soak the hemp seeds under the conditions described above. If the hemp is soaked in more amounts than the above range, there is a problem in that the hemp seeds sprout or are rather unsuitable for the growth of Cordyceps militaris.

[0021] In addition, in the method for culturing Cordyceps militaris of the present invention, the hemp mixed medium of step (2) can be prepared by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice and soaked hemp seeds, and then sterilizing at 110 to 130°C for 20 to 40 minutes, and more preferably, by adding 40 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice and soaked hemp seeds, and then sterilizing at 121°C for 30 minutes. Preparation of the hemp mixed medium under the above conditions is suitable for the growth of Cordyceps militaris, and can also increase the content of effective ingredients such as cordycepin and adenosine.

[0022] In addition, in order to further increase the palatability of the Cordyceps militaris cultured during the manufacture of the above-mentioned hemp mixed medium, lotus seed pulp may be additionally mixed. More specifically, 10 to 25 parts by weight of brown rice and 8 to 12 parts by weight of lotus seed pulp may be mixed with soaked hemp seeds, and even more specifically, 15 parts by weight of brown rice and 10 parts by weight of lotus seed pulp may be mixed with soaked hemp seeds.

[0023] In addition, in the method for culturing Cordyceps militaris of the present invention, the step (3) may preferably be performed by inoculating 15 to 25 parts by weight of Cordyceps militaris spores into a hemp mixed medium and culturing them at 19 to 21°C for 5 to 9 weeks, and more preferably by inoculating 20 parts by weight of Cordyceps militaris spores into a hemp mixed medium and culturing them at 19 to 21°C for 6 to 8 weeks. Culturing under the above conditions maximizes the content of effective ingredients of Cordyceps militaris, while also allowing sufficient growth in a state suitable for consumption in terms of flavor, taste, and texture.

[0024] More specifically, the method for cultivating Cordyceps militaris of the present invention is

[0025] (1) Step of soaking 25 to 50 parts by weight of hemp seeds in water at 20 to 25°C for 20 to 28 hours;

[0026] (2) A step of preparing a hemp mixed medium by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above, and then sterilizing at 110 to 130°C for 20 to 40 minutes; and

[0027] (3) It may include a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) above and culturing them at 19 to 21°C for 5 to 9 weeks.

[0028] More specifically

[0029] (1) Step of soaking 25 to 50 g of hemp seeds in water at 20 to 25°C for 20 to 28 hours;

[0030] (2) A step of preparing a hemp mixed medium by adding 30 to 50 mL of water to a mixture of 10 to 25 g of brown rice mixed with the soaked hemp seeds of step (1) above and sterilizing at 110 to 130°C for 20 to 40 minutes; and

[0031] (3) It may include a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) above and culturing them at 19 to 21°C for 5 to 9 weeks.

[0032] Most specifically

[0033] (1) Step of soaking 25 to 50 g of hemp seeds in water at 20 to 25°C for 24 hours;

[0034] (2) A step of preparing a hemp mixed medium by adding 40 mL of water to a mixture of 10 to 25 g of brown rice mixed with the soaked hemp seeds of step (1) above and sterilizing at 121°C for 30 minutes; and

[0035] (3) It may include a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them at 19 to 21°C for 6 to 8 weeks.

[0036] The present invention also provides Cordyceps militaris cultured by the above method.

[0037] The present invention also provides:

[0038] (1) Step of soaking hemp seeds in water;

[0039] (2) A step of preparing a hemp mixed medium by adding water to a mixture of brown rice and the hemp seeds of step (1) above and then sterilizing it; and

[0040] (3) A method for increasing the cordycepin and adenosine content of Cordyceps militaris is provided, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them.

[0041] The method for increasing the cordycepin and adenosine content of Cordyceps militaris of the present invention is more specifically:

[0042] (1) Step of soaking 25 to 50 parts by weight of hemp seeds in water at 20 to 25°C for 20 to 28 hours;

[0043] (2) A step of preparing a hemp mixed medium by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above, and then sterilizing at 110 to 130°C for 20 to 40 minutes; and

[0044] (3) It may include a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) above and culturing them at 19 to 21°C for 5 to 9 weeks.

[0045] More specifically

[0046] (1) Step of soaking 25 to 50 parts by weight of hemp seeds in water at 20 to 25°C for 24 hours;

[0047] (2) A step of preparing a hemp mixed medium by adding 40 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above, and then sterilizing at 121°C for 30 minutes; and

[0048] (3) It may include a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them at 19 to 21°C for 6 to 8 weeks.

[0049]

[0050] Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the content of the present invention is not limited to the following examples.

[0051]

[0052] Cultivation Example 1. Cordyceps militaris cultivation

[0053] (1) 25 g of unpeeled hemp seeds were soaked in water at 20-25℃ for 24 hours.

[0054] (2) A hemp mixed medium was prepared by adding 40 mL of water to a mixture of 25 g of unsoaked brown rice and the soaked hemp seeds of step (1) above, and sterilizing at 121°C for 30 minutes.

[0055] (3) 20 mL of Cordyceps militaris liquid spawn was inoculated into the hemp mixed medium prepared in step (2) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0056]

[0057] Cultivation Example 2: Cordyceps militaris cultivation

[0058] (1) 50 g of unpeeled hemp seeds were soaked in water at 20-25℃ for 24 hours.

[0059] (2) A hemp mixed medium was prepared by adding 40 mL of water to a mixture of 10 g of unsoaked brown rice and the soaked hemp seeds of step (1) above, and sterilizing at 121°C for 30 minutes.

[0060] (3) 20 mL of Cordyceps militaris liquid spawn was inoculated into the hemp mixed medium prepared in step (2) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0061]

[0062] Cultivation Example 3: Cordyceps militaris cultivation

[0063] (1) 25 g of unpeeled hemp seeds were soaked in water at 20-25℃ for 24 hours.

[0064] (2) A hemp mixed medium was prepared by adding 40 mL of water to a mixture of 15 g of unsoaked brown rice and 10 g of lotus seed, which were mixed with the soaked hemp seeds of step (1) above, and sterilizing at 121°C for 30 minutes.

[0065] (3) 20 mL of Cordyceps militaris liquid spawn was inoculated into the hemp mixed medium prepared in step (2) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0066]

[0067] Comparative Example 1. Cultivation of Cordyceps militaris

[0068] (1) Brown rice medium was prepared by adding 40 mL of water to 50 g of unsoaked brown rice and sterilizing at 121°C for 30 minutes.

[0069] (2) 20 mL of Cordyceps militaris liquid spawn was inoculated into the brown rice medium prepared in step (1) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0070]

[0071] Comparative Example 2. Cultivation of Cordyceps militaris

[0072] (1) 50 g of unpeeled hemp seeds were soaked in water at 20-25℃ for 24 hours.

[0073] (2) 40 mL of water was added to the soaked hemp seeds of step (1) above, and sterilized at 121°C for 30 minutes to prepare a hemp medium.

[0074] (3) 20 mL of Cordyceps militaris liquid spawn was inoculated into the hemp medium prepared in step (2) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0075]

[0076] Comparative Example 3. Cultivation of Cordyceps militaris

[0077] (1) 50 g of peeled hemp seeds were added with 40 mL of water and sterilized at 121°C for 30 minutes to prepare a hemp medium.

[0078] (2) 20 mL of Cordyceps militaris liquid spawn was inoculated into the hemp medium prepared in step (1) above, and cultured for 6 to 8 weeks under conditions of 19 to 21°C and 80 to 90% relative humidity.

[0079]

[0080] 1. Materials

[0081] The applicant of the present invention, 'Jang Sun-beom', holds a cannabis cultivation license, and the cannabis (Cannabis sativa L.) used was grown in a licensed facility.

[0082] The hemp variety used in the present invention is 'IH3', an industrial hemp with a hallucinogenic THC (Δ9-Tetrahydrocannabinol) content of 0.11%, which is significantly lower than the 1.74% of conventional varieties, and a hallucinogenic inhibitory CBD (Cannabidiol) content of 2.14%, which is higher than the 0.58% of conventional varieties. Therefore, although the IH3 variety is an industrial hemp with a THC content of less than 0.11%, no THC component is detected during the processing (sterilization, cultivation) process, making it safe.

[0083] In addition, the Cordyceps militaris used in the present invention was obtained from a mushroom cultivation bank.

[0084]

[0085] Example 1. Evaluation of Cordyceps militaris growth according to the amount of hemp seed soaking and water addition.

[0086] In a preliminary experiment, the inventor of the present invention found that when hemp seeds were used as a medium without being soaked, the Cordyceps militaris fruiting bodies were not properly cultured, so hemp seeds were soaked in water and used as a medium.

[0087] Hemp seeds with the outer hull removed can be used as a medium without soaking them in water, but when sterilized, they are not in the form of cooked rice, but rather in a rice cake-like state with a film formed, so there are no air gaps (pores) and the spawn cannot penetrate, preventing Cordyceps militaris from growing well. Therefore, they were used without removing the outer hull.

[0088] First, 50 g of hemp seeds were soaked in water at 20-25℃ for 48 hours, then taken out and washed in running water. 0 mL, 10 mL, 20 mL, and 30 mL of water were added to the seeds, respectively, and sterilized at 121℃ for 30 minutes to prepare a hemp medium. 20 mL of Cordyceps militaris liquid spawn was inoculated into the prepared hemp medium, and cultured for 6 weeks under conditions of 19-21℃ and 80-90% relative humidity. As a result, when 10 mL of water was added, the most Cordyceps militaris fruiting bodies were generated, but when 30 mL of water was added, a film was formed on the top and fruiting bodies were not formed. As a result, it was confirmed that soaking hemp seeds for 48 hours is not suitable for culturing Cordyceps militaris liquid spawn (Fig. 1).

[0089] Next, hemp seeds were soaked in water for 24 hours, taken out, and water was added in amounts of 10 mL, 20 mL, and 30 mL, respectively, and sterilized at 121°C for 30 minutes to prepare hemp medium. As a result of inoculating the liquid spawn into the hemp medium prepared above under the same conditions and culturing it, the occurrence of fruiting bodies increased as the amount of water added increased, but it was determined that only hemp seeds were insufficient in nutrients for the growth of Cordyceps militaris (Fig. 2).

[0090]

[0091] Example 2. Growth Evaluation of Cordyceps Militaris According to Mixture of Hemp and Brown Rice

[0092] A hemp mixed medium was prepared by mixing 25 g of hemp seeds soaked in water at 20-25℃ for 24 hours with 25 g of unsoaked brown rice, adding 40 mL of water, and sterilizing at 121℃ for 30 minutes. 20 mL of Cordyceps militaris liquid spawn was inoculated into each of the prepared hemp mixed medium, and after culturing for 8 weeks, it was confirmed that sufficient fruiting bodies of Cordyceps militaris were formed. Therefore, it was decided to prepare a medium by mixing hemp and brown rice for culturing Cordyceps militaris (Fig. 3). In addition, when preparing a medium by mixing brown rice, as in Cultivation Example 2, even if the ratio of hemp seeds was increased to 80% (hemp:brown rice = 8:2), the seeds grew well, so it was determined that the ratio of hemp seeds could be increased further.

[0093]

[0094] Example 3. Content of effective ingredients of Cordyceps militaris according to medium type

[0095] Cordyceps militaris cultured for 6 weeks using different types of media (Cultivation Example 1, Comparative Example 1) was subjected to experimental analysis to compare the contents of cordycepin and adenosine.

[0096] The detection method of cordycepin and adenosine was carried out by pretreating the sample included in the matrix and measuring the content of the main component in the pretreated sample using high-performance liquid chromatography equipped with a UV DAD (Diode Array Detector). The pretreatment method for analyzing the main component from Cordyceps militaris as the matrix was as follows: pulverize the main ingredient, then take a certain amount of powder sample, add it to a 50% methanol solution, and perform ultrasonic extraction for 1 hour using an ultrasonic device. The supernatant was taken and used as a test solution for high-performance liquid chromatography (HPLC) analysis.

[0097] In the measurement step of the above sample, the retention time of the peak, which is the measurement result, was measured using the elution time and absorbance of the peak component using the analysis equipment, high-performance liquid chromatography (HPLC). The absorbance was performed at 260 nm, and a comparative evaluation was performed based on whether the retention times of the main components in the standard solution and the sample solution were consistent. Acetonitrile and an aqueous solution containing 0.5% acetonitrile were used as the mobile phase. The mobile phase mixing ratio composition was 10% acetonitrile and 90% aqueous solution in the initial stage, and the separation of cordycepin and adenosine was confirmed by adjusting the mixing ratio of acetonitrile by time unit. The separation of each component was performed using a reversed-phase C18 column, with the column temperature set to 35℃ and the injection amount set to 15 ㎕.

[0098] Content of active ingredients of Cordyceps militaris according to substrate type (mg / g) Cordyceps militaris type Cordyceps militaris ...

[0099] As a result, cordycepin was not detected in the Cordyceps militaris of Comparative Example 1, but cordycepin was detected in the Cordyceps militaris of Cultivation Example 1, and it was confirmed that the adenosine content was increased by more than twice compared to Comparative Example 1. In addition, THC (Tetrahydrocannabinol), a hallucinogenic component of marijuana, was not detected in the cultivated Cordyceps militaris. These results suggest that the present invention can be a novel cultivation method that can increase the content of effective ingredients during Cordyceps militaris cultivation.

[0100]

[0101] Example 4. Sensory evaluation of Cordyceps militaris according to medium type

[0102] A sensory evaluation was conducted using Cordyceps militaris from cultivated and comparative examples. After each Cordyceps militaris was blanched in boiling water, 30 sensory evaluation agents were asked to classify the aroma, taste, texture, and overall preference. The results were evaluated on a 5-point preference scale (1 point: very bad, 4 points: bad, 3 points: average, 4 points: good, 5 points: very good) three times. The averages were calculated and compared in Table 2 below.

[0103] Sensory evaluation of Cordyceps militaris according to substrate type Type Aroma Taste Texture Overall preference Cultivation example 14.24.34.14.1 Cultivation example 24.34.34.14.2 Cultivation example 34.44.64.34.5 Comparative example 13.83.63.73.6 Comparative example 23.53.43.63.4 Comparative example 33.33.23.63.3

[0104] As a result, Cordyceps militaris of Comparative Examples 2 and 3, which were cultured on media using only hemp seeds or only hemp seeds, showed low scores in all items, and in the preliminary experiment, Cordyceps militaris did not grow well on media using only hemp seeds (Comparative Example 2) and only hemp seeds (Comparative Example 3), and was not suitable for growth. On the other hand, Cordyceps militaris cultured by the methods of the cultivation examples showed the highest scores in all items including aroma, taste, and texture, indicating that the conditions of the cultivation examples were most suitable for consumer preferences.

Claims

1. (1) Step of soaking hemp seeds in water; (2) a step of preparing a hemp mixed medium by adding water to a mixture of brown rice and the hemp seeds of step (1) above and then sterilizing it; and (3) A method for culturing Cordyceps militaris, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them.

2. In paragraph 1, (1) Step of soaking 25 to 50 weight parts of hemp seeds in water; (2) a step of preparing a hemp mixed medium by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above and then sterilizing the mixture; and (3) A method for culturing Cordyceps militaris, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium manufactured in step (2) and culturing them.

3. In paragraph 2, (1) A step of soaking 25 to 50 weight parts of hemp seeds in water at 20 to 25°C for 20 to 28 hours; (2) a step of preparing a hemp mixed medium by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above and sterilizing at 110 to 130°C for 20 to 40 minutes; and (3) A method for culturing Cordyceps militaris, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them at 19 to 21°C for 5 to 9 weeks.

4. Cordyceps sinensis cultured by any one of the methods of clauses 1 to 3. 5.(1) Step of soaking 25 to 50 weight parts of hemp seeds in water at 20 to 25°C for 20 to 28 hours; (2) a step of preparing a hemp mixed medium by adding 30 to 50 parts by weight of water to a mixture of 10 to 25 parts by weight of brown rice mixed with the soaked hemp seeds of step (1) above and sterilizing at 110 to 130°C for 20 to 40 minutes; and (3) A method for increasing the cordycepin and adenosine content of Cordyceps militaris, characterized by including a step of inoculating Cordyceps militaris spores into the hemp mixed medium prepared in step (2) and culturing them at 19 to 21°C for 5 to 9 weeks.

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