Composition for skin moisturization and skin microbiome improvement, comprising fructan

Abstract

The present invention relates to a composition for skin moisturization and skin microbiome improvement, comprising a fructan, and specifically to: a composition for skin environment improvement, comprising a fructan, a divalent polyol, and water; and a cosmetic composition comprising same. The compositions may have skin moisturization and skin microbiome improvement effects.

Description

Composition for moisturizing skin and improving skin microbiome containing fructan

[0001] The present invention relates to a composition for moisturizing the skin and improving the skin microbiome, including fructan.

[0002]

[0003] The skin is largely composed of three layers: the epidermis, the dermis, and the subcutaneous fat. The epidermis is divided, from outer to inner, into the stratum corneum, stratum lucidum, stratum granulosum, and stratum basale. The stratum corneum, the outermost layer of the epidermis, is the most important structure for the skin barrier function and is composed of anucleate, flat keratinocytes (corneocytes) and intercellular lipids (SC).

[0004] The stratum corneum, the outermost layer of the epidermis, contains 10-20% water and serves as the body's outermost layer. It prevents moisture evaporation from the body and prevents excessive moisture from penetrating from the outside. However, when the moisture content of the stratum corneum falls below 10%, the skin becomes rough and loses its barrier function.

[0005] The skin barrier not only protects against various external stimuli such as dry environments, ultraviolet rays, and pollutants, but also prevents excessive loss of body moisture through the skin. This protective function can only be maintained when the stratum corneum is formed normally.

[0006] Fructan is a general term for homopolysaccharides composed of fructose, and can be divided into inulin type in which D-fructofuranose is polymerized through a β2->1 linkage or levan type in which D-fructofuranose is polymerized through a β2->6 linkage, and glucose is bound to the reducing end.

[0007] Microbiome is a portmanteau of "microbe" and "biome," referring to the collective of microorganisms that coexist in and influence the human body. Some microorganisms are categorized as beneficial or harmful, while others collectively refer to specific areas of the body, such as intestinal bacteria or skin flora. When these bacteria coexist and maintain a balance, beneficial physiological effects can be expected. Recently, research, previously limited to intestinal flora, has expanded to include the skin, and skin-related research is actively being conducted not only in the cosmetics market but also in the treatment of skin diseases such as atopic dermatitis.

[0008] Accordingly, the inventors of the present invention developed a composition containing fructan, and confirmed its skin moisturizing ability and skin microbiome improvement ability, thereby completing the present invention.

[0009]

[0010] One aspect of the present invention is to provide a composition for improving skin environment, comprising 0.5 to 7 parts by weight of fructan, 20 to 35 parts by weight of digapolyol, and 58 to 79.5 parts by weight of water.

[0011] Another aspect of the present invention is to provide a cosmetic composition comprising the composition.

[0012]

[0013] One aspect of the present invention for achieving the above object is to provide a composition for improving skin environment, comprising 0.5 to 7 parts by weight of fructan, 20 to 35 parts by weight of digapolyol, and 58 to 79.5 parts by weight of water.

[0014] In one specific example of the present invention, the polyol may have 2 to 6 carbon atoms.

[0015] In one specific example of the present invention, the polyol may be propanediol or butylene glycol.

[0016] In one specific example of the present invention, the composition may contain 5 parts by weight of fructan, 30 parts by weight of digapolyol, and 65 parts by weight of water.

[0017] In one specific example of the present invention, the composition may improve the expression of one or more genes among FLG, IVL, and LoR.

[0018] In one specific example of the present invention, the composition may promote the growth of skin beneficial bacteria.

[0019] In one specific example of the present invention, the skin beneficial bacteria may be S. epidermidis.

[0020] Another aspect of the present invention provides a cosmetic composition comprising the composition.

[0021]

[0022] The composition and cosmetic composition of the present invention can provide skin moisturizing and skin microbiome improvement.

[0023]

[0024] Figure 1 is a drawing showing the results of confirming the TEWL value of the composition of the present invention.

[0025] Figure 2 is a drawing showing the results of confirming the moisturizing power of the composition of the present invention using a corneometer.

[0026] Figure 3 is a drawing showing the results of confirming the moisture level of the composition of the present invention through the amount of moisture evaporation.

[0027] Figure 4 is a graph showing the results of confirming changes in cell viability through the composition of the present invention.

[0028] Figures 5 to 7 are drawings showing the results of confirming the amount of mRNA expression related to moisturizing through the composition of the present invention.

[0029] Figure 8 is a drawing showing the results of confirming the microbiome improvement ability through the composition of the present invention at the level of biofilm formation.

[0030] Figure 9 is a drawing showing the results of confirming the growth effect of the microbiome improvement ability through the composition of the present invention.

[0031]

[0032] One aspect of the present invention provides a composition for improving skin environment, comprising 0.5 to 7 parts by weight of fructan, 20 to 35 parts by weight of digapolyol, and 58 to 79.5 parts by weight of water, and more specifically, the composition may comprise 3 to 8 parts by weight of fructan, 25 to 35 parts by weight of digapolyol, and 60 to 70 parts by weight of water, and in one example of the present invention, the composition may comprise 5 parts by weight of fructan, 30 parts by weight of digapolyol, and 65 parts by weight of water.

[0033] In one specific example of the present invention, the polyol may be a polyol having 2 to 6 carbon atoms, more specifically, propanediol or butylene glycol, for example, butylene glycol.

[0034] The above fructan is known to promote the growth of beneficial skin bacteria, and since digapolyol inhibits both beneficial and harmful skin bacteria when included in excess, the composition of the present invention is manufactured in the above ratio. Specifically, the effect of promoting beneficial bacteria growth was confirmed when fructan was included in an amount of 0.5% or more, and if it exceeds 7%, it does not dissolve, causing stability problems. Therefore, if fructan is included outside the above range, the effect of beneficial bacteria growth cannot be obtained, and stability may be problematic. Meanwhile, digapolyol has a risk of microbial contamination when it is included in an amount of less than 20%, and inhibits both beneficial and harmful bacteria when it is included in an amount of 35% or more.

[0035] The above 'improvement of skin environment' means directly / indirectly influencing to improve the internal / external conditions of the skin, and examples of improvement of skin environment may include moisturizing, UV protection, improvement of elasticity, improvement of skin microbiome, etc., and in the present invention, it may mean moisturizing and improvement of skin microbiome.

[0036] The above composition for improving skin environment can be manufactured by a known method.

[0037] The inventors of the present invention prepared the composition described above (Preparation Example 1) and confirmed its moisturizing level in various aspects. As a result, it was confirmed that it had a superior TEWL value (Fig. 1) and moisturizing level compared to common moisturizers such as hyaluronic acid and butylene glycol (Fig. 2), and that the moisture evaporation level was improved compared to butylene glycol (Fig. 3). In addition, it was confirmed that the composition improved skin moisturizing by increasing the expression of skin moisturizing-related genes (Figs. 5-7) without affecting cell growth (Fig. 4), and promoted the formation and growth of biofilms exclusive to skin beneficial bacteria (Figs. 8, 9).

[0038] In one specific example of the present invention, the composition may improve gene expression of one or more of FLG, IVL, and LoR.

[0039] The above FLG (Filaggrin), IVL (Involucrin), and LoR (Loricrin) are structural proteins that form the keratinocyte membrane. These structural proteins form a cross-linked membrane structure, which provides a skin protection function against the external environment and simultaneously inhibits moisture evaporation within the keratinocyte (Nat. Rev. Mol. Cell Biol. 6(4):328-340, 2005).

[0040] In one specific example of the present invention, the composition may promote the growth of skin beneficial bacteria.

[0041] Skin flora refers to bacteria located on the skin surface, and skin beneficial bacteria refers to strains of skin flora that help alleviate inflammation, control psoriasis-related inflammation, inhibit the growth of harmful bacteria, and inhibit the growth of tumors. The skin beneficial bacteria may be, for example, Staphylococcus epidermidis, but is not limited thereto. Staphylococcus epidermidis enhances skin defense by inducing the expression of antimicrobial peptides (AMPs) from epidermal cells and promoting skin regeneration; when treated with PSM (Phenol soluble modulin), a component secreted by Staphylococcus epidermidis, it inhibits the destruction (death) of cell membranes and growth of harmful bacteria, thereby promoting skin regeneration; butyric acid, a component secreted by Staphylococcus epidermidis, inhibits the expression of inflammatory factors induced by ultraviolet rays, thereby exhibiting a skin soothing effect; When the live bacteria are applied to the skin, they exhibit the effects of increasing skin moisture content and inhibiting moisture loss; when the live bacteria are applied to the skin, the skin pH becomes slightly acidic, thereby improving the pH environment; therefore, a substance having the effect of promoting Staphylococcus epidermidis, a beneficial bacterium, has a beneficial effect as a cosmetic composition. The "harmful bacteria" refers to bacteria that cause inflammatory reactions or acne on the skin, and examples thereof include Propionibacterium acnes, Corynebacterium minutissimum, and Staphlococcus aureus.

[0042] It was confirmed that the composition of the present invention inhibits biofilm formation of S. aureus, known as a skin resident bacteria, while promoting biofilm formation of S. epidermidis, known as a skin beneficial bacteria (Experimental Example 3-1). It was confirmed that the composition of the present invention promotes the growth of S. epidermidis, known as a skin beneficial bacteria, while not affecting the growth of S. aureus, known as a skin resident bacteria. It was confirmed that the composition of the present invention promotes the growth of S. epidermidis, known as a skin beneficial bacteria, while not affecting the growth of S. aureus, known as a skin resident bacteria (Experimental Example 3-2).

[0043]

[0044] Another aspect of the present invention provides a cosmetic composition comprising the composition.

[0045] In the cosmetic composition according to the present invention, the content of the composition is 0.01 to 20 wt%, preferably 0.1 to 10 wt%, based on the total weight of the cosmetic composition. Outside the above range, the desired effect may not be achieved, and skin inflammation may be caused. In addition, the cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to the composition for improving skin environment, which is an active ingredient, and such components include, for example, conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, and carriers. The cosmetic composition of the present invention may also be manufactured into a formulation commonly manufactured in the art, and may be formulated into, for example, a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, and a spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible toner, a nourishing toner, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

[0046] When the cosmetic composition formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. When the cosmetic composition formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether may be additionally included. When the cosmetic composition formulation of the present invention is a solution or emulsion, a solvent, a solubilizer or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. When the cosmetic composition formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like can be used as a carrier component. When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used as a carrier component.

[0047]

[0048] Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are provided for illustrative purposes only and the scope of the present invention is not limited to these examples.

[0049]

[0050] Manufacturing Example 1: Manufacturing of the composition of the present invention

[0051] After stirring butylene glycol and water at a ratio of 30:65 at room temperature, the weighed fructan (5 parts by weight) was slowly added and stirred at room temperature (400 to 500 rpm) to prepare the composition of the present invention.

[0052]

[0053] Experimental Example 1: Confirmation of the moisturizing level of the composition of the present invention

[0054] The moisturizing power of the composition of the present invention was confirmed.

[0055] Specifically, TEWL (Transepidermal water loss) was confirmed using the TM300 model. 100 ul of the sample (Fructan(levan)+BG+Water) was applied to the inner arm of 5 subjects under the conditions of 22.3℃ and 29.3% relative humidity, and then absorbed by rubbing with fingers. 10 consecutive measurements were taken per sample every 10 / 20 / 30 / 60 / 90 / 120 minutes, and the average and standard deviation were confirmed. Water, 1% hyaluronic acid (1% HA), and 30% butylene glycol were used as controls.

[0056] As a result, as confirmed in Fig. 1, when compared to hyaluronic acid and butylene glycol, which are common moisturizers, it showed excellent TEWL values ​​for 10 to 120 minutes after application.

[0057]

[0058] In addition, moisturizing power was confirmed using Corneometer (CM825). 100ul of the sample was applied to the inner arm of 5 subjects under the conditions of temperature 22.3℃ and relative humidity 29.3%, and then absorbed by rubbing with fingers. After measuring 4 times consecutively per sample every 10 / 20 / 30 / 60 / 90 / 120 minutes, the average and standard deviation were confirmed. Water, 1% hyaluronic acid (1% HA), and 30% butylene glycol (30% butylene glycol) were used as controls.

[0059] As a result, as confirmed in Fig. 2, butylene glycol (30%), a general moisturizer, showed a moisturizing level similar to water 90 minutes after application, and hyaluronic acid showed a moisturizing effect similar to water 20 minutes after application, whereas the composition of the present invention showed excellent moisturizing levels for up to 60 minutes from immediately after application of hyaluronic acid and butylene glycol.

[0060]

[0061] And, the moisture level was confirmed by checking the amount of moisture evaporation.

[0062] Specifically, each sample was accurately weighed in a 5g portion on a weighing dish and stored under constant temperature and humidity (25℃, 50% relative humidity) while observing the weight change. As a control group, 30% butylene glycol and 5% Levan powder (composition of 5(fructan):95(water)) were used.

[0063] As a result, as confirmed in Fig. 3, the composition of the present invention was confirmed to have a small weight loss (moisture evaporation level), and it was confirmed to absorb moisture in the air after 5 hours.

[0064]

[0065] Experimental Example 2: Confirmation of cell viability and mRNA expression level of the composition of the present invention.

[0066] The changes in cell viability and moisturizing-related gene expression levels through the composition of the present invention were confirmed.

[0067] Specifically, HaCaT cells were cultured in 10% FBS using a 96-well plate to prepare 1.8 x 10^4 cells. After treating the composition of the present invention at each concentration and the control group (PBS) for 24 hours, cell viability was confirmed, and under the same conditions, the mRNA expression levels of FLG (fillagrin), IVL (involucrin), and LOR (loricrin) were confirmed by RT-PCR.

[0068] As a result, as confirmed in Fig. 4, the composition of the present invention did not affect cell viability in the range of 0.25% to 1%, and as confirmed in Figs. 5 to 7, it was confirmed that the composition of the present invention increased the mRNA expression levels of FLG (fillagrin), IVL (involucrin), and LOR (loricrin) compared to the control group.

[0069] Through this, it can be seen that the composition of the present invention increases the mRNA expression levels of FLG (fillagrin), IVL (involucrin), and LOR (loricrin), thereby increasing the expression levels of proteins necessary for skin barrier formation, thereby protecting the skin and inhibiting moisture evaporation.

[0070]

[0071] Experimental Example 3: Confirmation of the Microbiome Improvement Ability of the Composition of the Present Invention

[0072] 3-1. Checking the level of biofilm formation

[0073] The microbiome improvement ability of the composition of the present invention was confirmed by changes in the level of biofilm formation of skin resident bacteria (S. aureus) and beneficial bacteria (S. epidermidis).

[0074] Specifically, S. aureus ATCC6538 and S. epidermidis ATCC12228 were inoculated into 50 ml of TSB and cultured for 18 hours to prepare. Using a 12-well plate, S. aureus and S. epidermidis were inoculated into 1.2 ml of TSB medium at 1% volume (v / v) and cultured for 4 to 5 days (80 to 100 rpm). At this time, the composition of the present invention was inoculated at 1% (v / v) to form a test group, and the same amount of distilled water was inoculated as a control group. After culturing for 4 to 5 days, when a biofilm was formed on the wall of the 12-well plate, the culture medium was carefully removed, and a 1% crystal violet solution was added to submerge the biofilm, followed by waiting at room temperature for 30 minutes.

[0075] After removing the crystal violet solution, the solution was washed three times with sterile water, the biofilm was dissolved with 1.5 ml of 96% ethanol, and the absorbance was measured at 590 nm to confirm the biofilm formation ability.

[0076] As a result, as confirmed in Fig. 8, it was confirmed that biofilm formation of S. aureus, known as a skin resident bacteria, was inhibited while biofilm formation of S. epidermidis, known as a skin beneficial bacteria, was promoted.

[0077]

[0078] 3-2. Checking the growth effect

[0079] The microbiome improvement ability of the composition of the present invention was confirmed by examining whether it affected the growth of skin flora (S. aureus) and beneficial bacteria (S. epidermidis).

[0080] Specifically, S. aureus ATCC6538 and S. epidermidis ATCC12228 were inoculated into 50 ml of TSB and cultured for 18 hours to prepare. The composition of the present invention was diluted in 0.85% NaCl to 1%, 3%, and 5%, and then each strain was diluted in 0.85% NaCl to inoculate each sample so that the final bacterial count was 10^6 CFU / g. After allowing to stand for 4 hours at 30°C, serial dilution was performed from 10^1 to 10^5 using 0.85% NaCl, and then 10ul each was spotted on TSA medium. Then, after culturing for 18 hours at 30°C, the grown bacteria were confirmed.

[0081] As a result, as confirmed in Fig. 9, it was confirmed that the growth of S. epidermidis, known as a beneficial skin bacterium, was promoted without affecting the growth of S. aureus, known as a skin resident bacteria.

[0082]

[0083] The present invention has been described above, focusing on preferred embodiments thereof. Those skilled in the art will appreciate that the present invention can be implemented in modified forms without departing from its essential characteristics. Therefore, the disclosed embodiments should be considered illustrative rather than limiting. The scope of the present invention is set forth in the claims, not the foregoing description, and all differences within the scope equivalent thereto should be construed as being encompassed by the present invention.

Claims

1. 0.5 to 7 parts by weight of fructan 20 to 35 parts by weight of igapolyol and Containing 58 to 79.5 parts by weight of water Composition for improving skin environment.

2. In paragraph 1, A composition for improving skin environment, characterized in that the above-mentioned igapolyol has 2 to 6 carbon atoms.

3. In paragraph 1, The above-mentioned igapolyol is a composition for improving the skin environment, which is propanediol or butylene glycol.

4. In paragraph 1, A composition for improving skin environment, comprising 5 parts by weight of fructan, 30 parts by weight of digapolyol, and 65 parts by weight of water.

5. In paragraph 1, The composition above is a composition that improves the expression of any one or more genes among FLG, IVL and LoR.

6. In paragraph 1, The above composition is a composition that promotes the growth of skin beneficial bacteria.

7. In paragraph 6, A composition wherein the above skin beneficial bacteria is S. epidermidis.

8. A cosmetic composition comprising the composition of paragraph 1.

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