Water-based disinfectant formulation and process for preparation of said formulation

Abstract

The present invention provides a water-based disinfectant formulation and process for the preparation thereof. The formulation includes a binding agent in a range of 0.1 % to 10 %, a cross-linking agent in a range of 0.1 % to 5 %, and an antimicrobial agent in a range of 0.1 % to 10 % based on 100% total volume of the formulation are mixed in water. The formulation includes water in a range of 90% to 98% based on 100% total volume of the composition. The formulation provides prolonged antimicrobial activity.

Description

WATER-BASED DISINFECTANT FORMULATION AND PROCESS FOR PREPARATION OF SAID FORMULATIONFIELD OF THE INVENTION

[0001] The present invention relates to disinfectant solutions. More particularly, the present invention relates to a water-based disinfectant formulation and process for preparing thereof. The water-based disinfectant according to present invention provides prolonged antimicrobial activity.BACKGROUND OF THE INVENTION

[0002] In recent times, there has been a notable increase in deaths caused by microbial actions, including pathogenic viruses, bacteria, and fungi. These microbial attacks have led to significant pandemics such as the recent COVID- 19 pandemic, as well as historical plagues like the Black Death, Spanish flu, and Cholera.

[0003] Within a short period, the current COVID-19 pandemic alone has claimed the lives of an estimated over 6.9 million people worldwide according to World Health Organization (WHO).

[0004] Additionally, individuals who have recovered from COVID- 19 have faced fatalities due to the growth of a fungal infection known as Black fungus.

[0005] Pathogenic microorganisms have been responsible for not only pandemics but also a multitude of other diseases. Surfaces such as clothing, seats, automobile interiors, vehicles, furniture, toys, bags, masks, packaging, and various other product surfaces serve as significant sources of disease transmission.

[0006] To combat this, disinfectants and sanitizers are commonly used and applied to surfaces to prevent the contamination and spread of pathogenic microorganisms, thereby controlling the spread of diseases.

[0007] High-concentration alcohol, including isopropyl alcohol and ethanol, has been widely used as a popular surface sanitizer.

[0008] One such alcohol-based disinfectant is disclosed in US patent no. 8703828. The ‘828 patent provides an antimicrobial therapy as a topical disinfectant.

[0009] The patent 828’ discloses an alcohol-containing antimicrobial composition that includes at least one paraben, a redox compound, and an organic acid at a concentration of from about 1.5 percent to about 10 percent by weight, based on the total weight of the composition.

[0010] However, these disinfectants / sanitizers are one-time use and only kill microorganisms at the point of application.

[0011] Consequently, such single-use products can be inconvenient, requiring frequent application, which is time-consuming, expensive, and complex.

[0012] Moreover, sanitizers / disinfectants often contain heavy metals and their compounds, such as silver, zinc, and copper, which have detrimental effects on the environment and human health. These materials also take a long time to naturally degrade.

[0013] Therefore, the present invention aims to address these challenges by describing a disinfectant formulation that is environmentally friendly, organic, long-lasting, water-based, alcohol-free, and free from heavy metals.

[0014] Further, there is a need for a formulation that exhibits antimicrobial properties against viruses, bacteria, fungi, and other microorganisms, providing an effective solution for surface disinfection.

[0015] In a nutshell, a disinfectant formulation and process are required that may overcome the drawbacks discussed above and provide an efficient and cost-effective way of disinfecting surfaces with prolonged antimicrobial activity.SUMMARY OF THE INVENTION

[0016] In an aspect of the present invention, a water-based disinfectant formulation that provides prolonged antimicrobial activity is disclosed.

[0017] In one embodiment of the present invention, the water-based disinfectant formulation includes an aqueous solvent, specifically water in a range of 90% to 98% by weight, more specifically 96% based on 100% by weight of total volume of the formulation.

[0018] The water-based disinfectant formulation includes a binding agent added into the water in a range of 0.1 % to 10 % by weight based on 100% total volume of the formulation.

[0019] More specifically, the binding agent is selected from one of polyvinyl alcohol or any similar polyvinyl soluble material.

[0020] In one embodiment of the present invention, the disinfectant formulation further includes a cross-linking agent that is added to the water in a range of 0.1 % to 5 % by weight based on 100% totalvolume of the formulation. More specifically, the cross-linking agent selected is an organic acid, such as citric acid.

[0021] However, it should be noted that the binding agent and crosslinking agent are not limited to the above-mentioned materials. The binding agent and the crosslinking agent may include but are not limited to citrates, sugar molecules, acrylates, acrylic compounds, alkyd resins, and other polymer binders.

[0022] In the above embodiments of the present invention, the binding agent and the cross-linking agent are mixed in the water, followed by heating the mixed solution in a temperature range of 60-120 degrees Celsius for 1-4 hours.

[0023] The disinfectant formulation further includes an antimicrobial agent mixed into the mixed solution of the binding agent, the cross-linking agent, and the water.

[0024] The antimicrobial agent is selected from but is not limited to at least one of the organic Quaternary Ammonium compounds, Dodecyl Dipropylene Triamine, Benzalkonium chloride, Nitrogencontaining organic biocides, and Benzalkonium Halides.

[0025] In another aspect of the present invention, a process for preparing the water-based disinfectant formulation is disclosed.

[0026] The process involves a step of adding the binding agent and the cross-linking agent to the water in a range of 0.1 % to 10 % and 0.1 % to 5 % respectively based on 100% total volume of the disinfectant formulation.

[0027] Further, the process involves a step of heat treating the mixture solution of the binding agent and the cross-linking agent in a temperature range of 60 -120 degrees Celsius for 1-4 hours for uniformly mixing of the solution.

[0028] Further, the said uniformed mixed solution is added with the antimicrobial agent in a range of 0.01% to 10 %, more preferably, 2 % based on 100% weight of the formulation, along with the stabilizing agent in a range of 0.01 % to 1 % to form the disinfectant formulation.

[0029] The antimicrobial agent is selected from but not limited to at least one of the organic Quaternary Ammonium compounds, Dodecyl Dipropylene Triamine, Benzalkonium chloride, and Nitrogencontaining organic biocides.

[0030] In an exemplary embodiment of the present invention, a secondary antimicrobial agent is added to the formulation which is selected from one of Benzalkonium halides as secondary QAC and sulfur nanoparticles for additional strength of antimicrobial power whenever required.

[0031] “Stabilizing agents” are inert substances that modify the physicochemical properties of aqueous solutions used to preserve biomolecules.

[0032] The stabilizing agents are comprised of but not limited to surfactants, fatty acids with their salts, poly-electrolytes, and any other stabilizing agent suitable for biocide.

[0033] In yet another aspect of the present invention, a method of disinfection is disclosed. The method involves applying the disinfectant formulation to a targeted area such as at least one of the vehicle interiors, furniture, benches, seats, pillows, floors, walls, wooden items, cloths, tables, containers, and devices.

[0034] The disinfectant formulation is applied on the targeted area by one of spray techniques, coating techniques, and dipping.

[0035] This, along with the other aspects of the present invention and the various features of novelty that characterize the present disclosure, is pointed out with particularity.

[0036] For a better understanding of the present disclosure, its operating advantages, and the specified objective attained by its uses, reference should be made to the accompanying descriptive matter in which there are illustrated exemplary embodiments of the present invention.BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The advantages and features of the present invention will become better understood with reference to the following detailed description taken in conjunction with the accompanying drawings, in which:

[0038] Fig. 1 illustrates a flowchart representing a process for preparing a water-based disinfectant formulation, according to various embodiments of the present invention;

[0039] Fig. 2 illustrates a schematic diagram representing disinfection of microbes from the surface by utilizing the water-based disinfection formulation, according to various embodiments of the present invention;

[0040] Fig. 3 illustrates a table representing test results which show that there is no detection of any heavy metals in the water-based disinfection formulation, according to various embodiments of the present invention;

[0041] Figs. 4A-4B illustrate test results depicting antibacterial property after 24 hours for an infected surface is treated with the disinfection formulation, according to various embodiments of the present invention;

[0042] Fig. 5 illustrates test results depicting antibacterial property after 24 hours for an infected plastic surface treated with the disinfection formulation, according to various embodiments of the present invention;

[0043] Figs. 6A-6B illustrate test results depicting antimicrobial property after 30 washing cycles for an infected fabric treated with the disinfection formulation, according to various embodiments of the present invention; and

[0044] Fig. 7 illustrates test results depicting antifungal property of the disinfection formulation, according to various embodiments of the present invention; and

[0045] Fig. 8 illustrates test results depicting virucidal property of the disinfection formulation against COVID-19, according to various embodiments of the present invention.

[0046] Like numerals denote like elements throughout the figures.DETAILED DESCRIPTION OF THE INVENTION

[0047] The exemplary embodiments described herein detail for illustrative purposes are subjected to many variations. It should be emphasized, however, that the present invention is not limited to as disclosed.

[0048] It is understood that various omissions and substitutions of equivalents are contemplated as circumstances may suggest or render expedient, but these are intended to cover the application or implementation without departing from the scope of the present invention.

[0049] Specifically, the following terms have the meanings indicated below.

[0050] The terms “a” and “an” herein do not denote a limitation of quantity, but rather denote the presence of at least one of the referenced items.

[0051] The terms “having”, “comprising”, “including”, and variations thereof signify the presence of a component.

[0052] The inventive aspects of the invention along with various components and engineering involved will now be explained with reference to Figs. 1-6 herein.

[0053] In an aspect of the present invention, a water-based disinfectant formulation that provides prolonged antimicrobial activity is disclosed.

[0054] In one embodiment of the present invention, the water-based disinfectant formulation includes an aqueous solvent, specifically water in a range of 90% to 98% weight by volume, more specifically 96% based on 100% total volume of the formulation.

[0055] The water-based disinfectant formulation further includes a binding agent added into the water in a range of 0.1 % to 10 % by weight based on 100% total volume of the formulation.

[0056] More specifically, the binding agent is selected from one of a polymeric alcohol, such as polyvinyl alcohol or any similar polyvinyl soluble material.

[0057] In one embodiment of the present invention, the disinfectant formulation further includes a cross-linking agent that is added to the water in a range of 0.1 % to 5 % by weight based on 100% total volume of the formulation.

[0058] More specifically, the cross-linking agent selected in the present invention is an organic acid, such as citric acid.

[0059] However, it should be noted that the binding agent and crosslinking agent are not limited to the above-mentioned material. The binding agent and the crosslinking agent may include but are not limited to citrates, sugar molecules, acrylates, acrylic compounds, alkyd resins, and other polymer binders.

[0060] In the above embodiments of the present invention, the binding agent and the cross-linking agent are mixed together in the water, followed by heating the mixed solution in a temperature range of 60-120 degrees Celsius for 1-4 hours.

[0061] The disinfectant formulation further includes an antimicrobial agent mixed into the mixed solution of the binding agent, the cross-linking agent, and the water.

[0062] The antimicrobial agent is selected from but is not limited to at least one of the organic Quaternary Ammonium compounds, Dodecyl Dipropylene Triamine, Benzalkonium chloride, Nitrogencontaining organic biocides, and Benzalkonium Halides.

[0063] The water-based formulation further includes a stabilizing agent added in a range of 0.01% to 1%, and preferably 1% based on 100% total volume of the disinfectant formulation.

[0064] The stabilizing agents are comprised of but not limited to surfactants, fatty acids with their salts, poly-electrolytes, and any other stabilizing agent suitable for biocide.

[0065] In another aspect of the present invention, a process (50) for preparing the water-based disinfectant formulation is disclosed.

[0066] Referring to Fig. 1, at step (10), the process (50) involves a step of adding the binding agent and the cross-linking agent to the water in a range of 0.1 % to 2 % and 0.1 % to 1 % respectively based on 100% total volume of the disinfectant formulation.

[0067] Further, at step (12), the process (50) involves a step of heat treating the mixture solution of the binding agent and the cross-linking agent in a temperature range of 60 -120 degree Celsius for 1-4 hours for uniformly mixing of the solution.

[0068] Further, the said uniformed mixed solution is added with the antimicrobial agent in a range of 0.01 to 10%, more preferably, 2 % based on 100% weight of the formulation, along with the stabilizing agent in a range of 0.01 % to 5 %, preferably 1% to form the disinfectant formulation, as shown in steps 14 and 16, respectively (Refer to Fig. 1).

[0069] The antimicrobial agent is selected from but not limited to at least one of the organic Quaternary Ammonium compounds, Dodecyl Dipropylene Triamine, Benzalkonium chloride, and Nitrogencontaining organic biocides.

[0070] In an exemplary embodiment of the present invention, a secondary antimicrobial agent is added to the formulation which is selected from one of Benzalkonium halides as secondary QAC and sulfur nanoparticles for additional strength of antimicrobial power whenever required.

[0071] “Stabilizing agents” are inert substances that modify the physicochemical properties of aqueous solutions used to preserve biomolecules.

[0072] The stabilizing agents are comprised of but not limited to surfactants, fatty acids with their salts, poly-electrolytes, and any other stabilizing agent suitable for biocide.

[0073] In the embodiment of the present invention, examples showing the preparation of the disinfectant solution are described in the below paragraphs. The following examples are illustrative of the present invention, however, it will be understood that the invention is not limited to the specific details set forth in the examples.Example 1:

[0074] The first example involves completely dissolving 1 g of polyacrylic acid and 0.5 g of citric acid in 1 L of water. Then adding 2 mL of antimicrobial quaternary ammonium salt to the solution together with 0.01 g of poly acrylate. The resulting solution is mixed well till the formation of a clear solution. This product is used as a disinfectant spray or bath for dip coat products to be converted into antimicrobial surfaces. Any water-soluble fragrance can be added to make it pleasant smell of the product.Example 2:

[0075] The second example involves completely dissolving 1 g of polyacrylic acid and 0.5 g of citric acid in 1 L of water. Then 2 mL of each antimicrobial quaternary ammonium salts (dodecyl dipropylene triamine and, Benzalkonium chloride) are added, and sulfur nanoparticles are also added to the solution together with 0.01 g of polyacrylate, and the resultant solution is mixed well till the formation of a clear solution. This product is used as a high-strength antimicrobial disinfectant spray or bath for dip coat products to be converted into antimicrobial surfaces. Any water-soluble fragrance can be added to make it pleasant smell of the product.Example 3:

[0076] In the third example, 1 g of polyvinyl alcohol and 0.5 g of sodium citrate are dissolved at a temperature of 80C, in 1 L of water. Thereafter, 2 mL of antimicrobial quaternary ammonium salt is added to the solution together with 0.01 g of poly acrylate. The resultant solution is mixed well till the formation of a clear solution. In the solution, any water-soluble fragrance, 1-5% of the cleaning compound, Sodium laureth sulfate, may also be added. This product can be used as a disinfectant Cleaner or laundry liquid.

[0077] Referring to Fig. 3, the water -based disinfectant formulation [as in examples] of the present invention is tested for heavy metals in a laboratory. The test was conducted by Bureau Veritas Consumer Product Services Lanka (PVT) Ltd. The summary of the test results are provided in Fig. 3. According to the results, no tracing of heavy metals is found in the formulation. This is highly advantageous effect of the invention.

[0078] In yet another aspect of the present invention, a method for disinfectant is disclosed.

[0079] The method for the disinfection includes steps of applying the disinfection formulation as discussed in the above paragraphs over a targeted area such as vehicle interiors, furniture, benches, seats, pillows, floors, walls, wooden items, cloths, tables, containers, and devices.

[0080] The disinfectant formulation may be applied on the surfaces of the targeted area via spray techniques, coating techniques, dipping, or the like.

[0081] More specifically, if the water-based formulation is sprayed, the formulation advantageously may be supplied in a conventional bottle with a sprayer. The sprayer may be a trigger sprayer. To disinfect a contaminated surface, the liquid formulation is sprayed until the target area is completely covered. The wet formulation subsequently may be wiped dry with a dry cloth or paper towel.

[0082] In the embodiment of the present invention, the use of polyvinyl alcohol or similar polyvinyl soluble material as the binding agent and citric acid as the cross-linking agent enables an attachment of the antimicrobial agent on the surfaces of the targeted area.The binding technique as disclosed in the paragraph above, allows the disinfectant formulation to show a prolonged action against bacteria, viruses including COVID- 19 virus, funguses, and any other microorganism with 1 min - 45 days or more time with the one-time application, depending on surface chemistry of the targeted area.

[0083] More specifically, in long duration on surface activeness, the antimicrobial compounds / agents in the disinfectant formulation are attached to the surface through a combination of the binder and the crosslinker. The bonding happens mainly with chemical attachment including Vander Waals forces, coordination bonding and also hydrogen bonding. Therefore, the antimicrobial compound / agent is strongly attached to the surface and covers the surface as an invisible shield in protecting germs (Refer to Fig- 2).

[0084] The experimental analysis of the disinfectant formulation of the present invention is discussed in the below paragraphs.Product name. VIS-Viroshield liquid disinfectant (water-based disinfectant formulation) sprayed metal, plastic, and glass pieces.Objective of the Experiment: To test the antibacterial properties of VIS-Viroshield liquid disinfectant applied metal, plastic, and glass pieces using bacterial species of Staphylococcus aureus and Escherichia coli after 45 days of application.Test protocol: ISO 22196:2011(E)Test Procedure: Metal, plastic, and glass samples were treated with VIS-Viroshield liquid disinfection 45 days before the experiments. Three treated test specimens (Viroshield applied metal, Viroshield applied plastic, and Viroshield applied glass) and three untreated metal, glass, and plastic specimens (controls) were used in the experiment, and triplicates were maintained for each treatment. The sample surfaces were sterilized prior to testing. Then, the test specimens were placed in a separate sterile Petri dish with the test surface uppermost. Thereafter, a bacterial test inoculum of 0.4 ml (Staphylococcus aureus or Escherichia coli) was pipetted to the test surface and covered with a cover film (40 mm x 40 mm). The number of colony-forming units (CFU) on test materials was counted immediately after incubation and (Oh) after 24 h (24h) incubation at 35°C for both control and tested samples. Then microbial counts were estimated using the standard serial dilution technique followed by growing on bacteriological media (ISO 4833 -Enumeration of Microorganisms; Colony Count Technique).

[0085] The antibacterial activity of samples R was calculated using the following formula:R = (Ut-U0)~(At-U0), Here, ‘U’ and ‘A’ were the average of the common logarithm of the number of viable bacteria enumerated from untreated and treated surfaces. Whereas respectively, ‘0° and’ corresponds to the 0 and 24 h inoculation times tested. A sample was considered as active only if R is higher than one (1).

[0086] Referring to Table-1 below, the testing results on the water-based disinfectant formulation of the present invention, demonstrate the desired residual sanitizing or disinfecting performance once applied onto surfaces and dried. This is advantageous effect of the invention.

[0087] More specifically, based on the results, by following the protocol ISO 22196:201 1(E), VIS- Viroshield liquid disinfectant (water -based disinfectant formulation) applied metal, plastic, and glass surfaces indicated antibacterial properties against Staphylococcus aureus and Escherichia coli after 45 days of application.Table- 1

[0088] Referring to Figs. 4A-4B, there is shown test results that show that the surface treated by the disinfection formulation shows antibacterial property. The treated surface is tested after 24 hours for bacteria Staphylococcus aureus and bacteria Klebsiella pneumoniae by utilizing Plate count method.

[0089] More specifically, 99.98 % reduction of the bacteria Staphylococcus aureus and a 99.95 % reduction of bacteria Klebsiella pneumoniae (Refer Figs. 4A-4B).

[0090] Referring to Fig. 5, there is shown test results that show that the plastic surface treated by the disinfection formulation shows antibacterial property. The treated plastic surface is tested after 24 hours for bacteria Staphylococcus aureus and bacteria Escherichia coli. The details of the test is shown below:Test organism - Escherichia coli (ATCC 8739)Final concentration of the culture - 1.5x105 CFU / mLStaphylococcus aureus (ATCC 6538P)Final concentration of the culture - 1.3x105 CFU / mLSample: Coated SampleContact time: 24 Hours

[0091] More specifically, 99.99 % reduction of the bacteria Staphylococcus aureus and a 99.99 % reduction of bacteria Klebsiella pneumoniae (Refer Fig. 5).

[0092] Further, a foam which is treated by the disinfection formulation is tested for antifungal property.The details of the test is shown Table -2 and Fig. 7 below:Table-2

[0093] Further, the disinfectant formulation is tested for detecting the efficacy of the said formulation against the COVID-19 virus, preferably against Human coronavirus, strain 229E, ATCC VR-740.

[0094] Specifically, Fig. 8 shows the test results of Viroxy Labs, Malaysia depicting the efficacy of the water based disinfectant formulation against COVID-19 virus. The test results shows viral reduction of >4.0 log 10 against test strain Human coronavirus ATCC VR-740 in accordance after 5 and 60 minutes under test conditions.

[0095] The virucidal activity of the disinfectant formulation (VISViroshield) against Human coronavirus ATCC VR-740 was investigated by a quantitative suspension test according to EN 14476:2013+A2:2019 (E) under clean condition (0.30 g / L bovine albumin solution).

[0096] According to this suspension test, the disinfectant formulation at a particular concentration is considered as having virucidal activity if the virus titre is reduced by > 4 loglO (inactivation >99.99 %) within the recommended exposure period.

[0097] The disinfectant solution was examined at 20 °C at the concentration of 100.00 %** for the exposure times of 5 and 60 minutes. After the exposure times, the viral reduction exceeded 4 loglO-steps in all assays. According to the simple acceptance decision rule, there is a minimal risk of false acceptance. Therefore, a virucidal activity against Human coronavirus ATCC VR-740 was measured as follows:Clean condition 100.00 %** 5 minutesClean condition 100.00 %** 60 minutes

[0098] More specifically, referring to Table-3, a summary of the log reductions of the quantitative suspension test according to EN 14476 is shown below:Table-3

[0099] In one embodiment of the present invention, the disinfectant formulation may also be mixed with softeners and cleaning agents to develop various products like antimicrobial fabric conditioners and laundry liquids.

[0100] The antimicrobial fabric conditioners and laundry liquids as produced may provide 30 or more washing cycles on textiles & garments.

[0101] In the embodiment of the present invention, a test is conducted after 30 washing cycles on a fabric treated with the disinfectant formulation of the present invention.

[0102] Referring to Figs. 6A-6B, illustrate test results that show that the treated fabric showed antimicrobial activity even after 30 washing cycles, more specifically 99.4 % reduction of the bacteria Staphylococcus aureus and a 99.31 % reduction of bacteria Klebsiella pneumoniae.

[0103] Furthermore, the disinfectant formulation may also be used as an alcohol-free hand sanitizer.Advantageous Effects of the Invention

[0104] The disinfectant formulation of the present invention, uses the antimicrobial agents / compounds such as quaternary ammonium salts to kill microorganisms, especially fungi, and these antimicrobial compounds are also soluble in water, which makes the disinfectant formulation irritation-free and low in cost.

[0105] The disinfectant formulation of the present invention, provides prolonged antimicrobial activity on the formulation-sprayed surfaces.

[0106] The disinfectant formulation of the present invention, kills viruses bacteria, and fungi on the surface and avoids their further growth due to prolonged action.

[0107] Further, the formulation may withstand over 30 detergent washing cycles of textiles and garments and antimicrobial activity remains the same after 30 washing cycles.

[0108] Moreover, the disinfectant formulation of the present invention is Heavy metals-free waterbased, bio-degradable, and transparent.

[0109] In a nutshell, the disinfectant formulation of the present invention overcomes the drawbacks discussed above and provides an efficient and cost-effective way of disinfecting surfaces with prolonged antimicrobial activity.

[0110] It is understood that various omissions and substitutions of equivalents are contemplated as circumstances may suggest or render expedient, but such omissions and substitutions are intended to cover the application or implementation without departing from the spirit or scope of the present invention.

[0111] The foregoing descriptions of specific embodiments of the present invention have been presented for purposes of description. They are not intended to be exhaustive or to limit the present invention to the precise forms disclosed, and obviously, many modifications and variations are possible in light of the above teaching.

[0112] Further, the embodiments were chosen and described to best explain the principles of the present invention and its practical application, and thereby enable others skilled in the art to best utilize the present invention and various embodiments with various modifications as are suited to the particular use contemplated.

Claims

CLAIMS:

1. A water-based disinfectant formulation comprising: a solvent comprising water in a range of 90% to 98% based on 100% total volume of the composition; a binding agent comprising at least one biodegradable polymer added into the solvent in a predefined weight percentage of 0.1 % to 5 %; a cross-linking agent comprising at least one organic acid added into the solvent in a predefined weight percentage of 0.1 % to 1 % based on 100% total volume of the formulation; and an antimicrobial agent comprising at least one Quaternary Ammonium compound added in a predefined weight percentage of 0.01% to 10 % based on 100% total volume of the formulation, to a mixed solution of the binding agent, the cross-linking agent, and the solvent.

2. The formulation as claimed in claim 1 , wherein the binding agent and the crosslinking agent are organic binders and are selected from at least one of citrates, sugar molecules, acrylates, acrylic compounds, alkyd resins, and other polymer binders.

3. The formulation as claimed in claim 1 comprising at least one stabilizing agent and dispersing agent in a range of 0.01 % to 1% based on 100% total volume of the formulation, wherein the stabilizing agent is selected from at least one of surfactants, fatty acids with their salts and poly -electrolytes.

4. The formulation as claimed in claim I wherein the antimicrobial agent is selected from at least one of organic Quaternary Ammonium compounds, Dodecyl Dipropylene Triamine, Benzalkonium chloride, Nitrogen-containing organic biocides, Benzalkonium Halides, and Sulfur Nanoparticles.

5. A process for preparing a water-based disinfectant formulation, wherein the process comprises steps of: adding a binding agent comprising at least one biodegradable polymer into water in a predefined weight percentage of 0.1 % to 10 % based on 100% total volume of the formulation; adding a cross-linking agent comprising at least one organic acid into the water in a predefined weight percentage of 0.1 % to 5 % based on 100% total volume of the formulation; treating a mixed solution of the binding agent, the cross-linking agent, and the water at a temperature range of 60 - 120 degrees Celsius for about 1-3 hours; and adding an antimicrobial agent comprising at least one quaternary ammonium compound to the solution in a predefined weight percentage of 0.01% to 10 % based on 100% total volume of the formulation, thereby obtaining the said formulation.

6. The process as claimed in claim 5 comprising uniformly mixing the binding agent and the crosslinking agent prior to treatment of the binding agent and cross-linking agent in water.

7. The process as claimed in claim 6, wherein the binding agent and the crosslinking agent are organic binders selected from at least one of citrates, sugar molecules, acrylates, acrylic compounds, alkyd resins, and other polymer binders.

8. The process as claimed in claim 6 wherein the antimicrobial agent is selected from at least one of organic quaternary ammonium compounds, Dodecyl Dipropylene triamine and, Benzalkonium chloride and nitrogen-containing organic biocides.

9. The process as claimed in any one of claims 5 or 8 further comprising adding a secondary antimicrobial agent, wherein the secondary antimicrobial agent is Benzalkonium Halides.

10. The process as claimed in any one of claims 5-9 further comprising adding nanoparticles such as sulfur nanoparticles.

11. The process as claimed in any one of claims 5-10 further comprising adding at least one of a stabilizing agent or a dispersing agent post-addition of the antimicrobial agent in a range of 0.01% to 10% based on 100% total volume of the formulation, wherein the stabilizing agent and the dispersing agent surfactants, fatty acids with their salts, poly-electrolytes and any other stabilizing agent suitable for biocide.

12. A method of disinfection comprising applying the disinfectant formulation claimed in any one of claims 1-4 to a targeted area.

13. The method as claimed in claim 12, wherein the disinfectant formulation is applied on the targeted area by one of the spray techniques, coating techniques, and dipping.

14. The method as claimed in any one of claims 12 or 13, wherein the targeted area comprises at least one of vehicle interiors, furniture, benches, seats, pillows, floors, walls, wooden items, cloths, tables, containers, and devices.

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