Integrated nucleic acid extraction and amplification testing device

Abstract

Provided is an integrated nucleic acid extraction and amplification testing device, comprising a bracket, an amplification apparatus, an extraction apparatus, a fluorescence detection apparatus and a driving apparatus. The amplification apparatus is disposed on the bracket, and is used to perform temperature adjustment; the extraction apparatus is mounted on the amplification apparatus, and the extraction device comprises a test tube mounting assembly, a first driving member and a magnetic attraction component, the test tube mounting assembly having a first mounting hole, the magnetic attraction component being disposed on a hole wall of the first mounting hole, and the first driving member being used to drive the test tube mounting component to rotate, thereby driving the magnetic attraction component to rotate around an outer peripheral side of a test tube located in the test tube mounting assembly and perform nucleic acid extraction; the fluorescence detection apparatus is mounted on the bracket, and is used to perform fluorescence detection of the test tube; an output end of the driving apparatus is connected to the amplification apparatus, and is used to drive the amplification apparatus and the extraction apparatus to rotate relative to the fluorescence detection apparatus. By integrating the functions of nucleic acid extraction, amplification and fluorescence detection into a single device, efficiency is improved and testing time is shortened.

Description

A nucleic acid extraction and amplification integrated detection device Technical Field

[0001] The present invention relates to the field of nucleic acid technology, in particular to a nucleic acid extraction and amplification integrated machine detection device. Background Art

[0002] At present, the steps of nucleic acid extraction, amplification, and detection are all completed manually in the laboratory. A nucleic acid test requires multiple steps such as extraction, amplification, PCR fluorescence detection, etc., and to prevent aerosol contamination and biosafety, the existing nucleic acid extraction process and amplification PCR detection operations are completed in laboratories in different partitions, requiring the use of multiple devices such as nucleic acid extractors and PCR amplifiers. Each device is a single product. Different operation steps and processes are performed manually in multiple independent partitions and different devices, which increases the complexity and tediousness of the nucleic acid extraction and detection process, making manual operation inevitable. Operational errors will occur. At the same time, the number of nucleic acid extraction tests that can be performed manually at one time is small and the time required is long, which further makes the efficiency of manual operation low. Completing different steps in laboratories in different partitions cannot effectively avoid aerosol contamination, and the safety is low.

[0003] Summary of the Invention

[0004] The technical problem to be solved by the present invention is that the extraction, amplification and detection of nucleic acids are completed in laboratories in different partitions, which is a cumbersome process, has low operational efficiency and cannot effectively avoid aerosol contamination.

[0005] In order to solve the above technical problems, the present invention provides a nucleic acid extraction and amplification integrated detection device, comprising:

[0006] Bracket;

[0007] an amplification device, disposed on the support and used for regulating temperature;

[0008] An extraction device is mounted on the amplification device, comprising a test tube mounting assembly, a first driving member, and a magnetic member. The test tube mounting assembly has a first mounting hole, and the magnetic member is disposed on a wall of the first mounting hole. The first driving member is mounted on the bracket and is configured to drive the test tube mounting assembly to rotate, thereby driving the magnetic member to rotate around the outer circumference of the test tube located in the test tube mounting assembly and extract nucleic acid.

[0009] a fluorescence detection device, mounted on the bracket, for performing fluorescence detection on the test tube; and

[0010] A driving device is installed on the bracket, and an output end of the driving device is connected to the amplification device, and is used to drive the amplification device and the extraction device to rotate relative to the fluorescence detection device.

[0011] In some embodiments, the extraction device further comprises a gear plate, and the output shaft of the first driving member passes through the amplification device and is connected to the gear plate;

[0012] The test tube mounting assembly includes a gear component, the gear component has the first mounting hole, the gear plate is engaged with the gear component, and the magnetic component is arranged on the gear component.

[0013] In some embodiments, the first mounting hole passes through both ends of the gear component, a second mounting hole is formed in the wall of the first mounting hole, and the magnetic component is disposed in the second mounting hole.

[0014] In some embodiments, the extraction device further includes a bottom plate and a top plate, a receiving cavity is formed between the bottom plate and the top plate, the gear plate is arranged in the receiving cavity, the bottom plate is arranged on the amplification device, and a third mounting hole is provided on the bottom plate for the bottom of the test tube to pass through.

[0015] In some embodiments, a fourth mounting hole is provided on the end surface of the gear member facing the bottom plate, and a fifth mounting hole is provided on the end surface of the gear member facing the top plate, and balls are provided in both the fourth mounting hole and the fifth mounting hole.

[0016] The test tube mounting assembly further includes a matching piece, the matching piece being arranged on the bottom plate, the matching piece being provided with a first slide groove on a side facing the top plate, the third mounting hole passing through the first slide groove; the top plate being provided with a second slide groove on a side facing the bottom plate corresponding to the gear member;

[0017] The gear component is arranged between the top plate and the matching component. The gear component and the matching component are arranged correspondingly, and the gear component can rotate on the first sliding groove and the second sliding groove through the ball bearing.

[0018] In some embodiments, the test tube mounting assembly further includes a first heating element, which is disposed on the top plate and corresponds to the gear element.

[0019] In some embodiments, the test tube mounting assembly further includes a heat insulating member, which is sleeved on the outer circumference of the first heating member.

[0020] In some embodiments, there are multiple test tube mounting assemblies, and the multiple test tube mounting assemblies are arranged around the circumference of the gear plate to form a test tube mounting group;

[0021] The number of the test tube installation groups is n, and the n test tube installation groups are arranged in sequence along the radial direction of the gear plate, wherein n≥1.

[0022] In some embodiments, the amplification device includes a heating plate, a second heating element and a heat conductor, the second heating element is arranged on the heating plate and located below the extraction device, and the heat conductor and the first driving element are arranged on the side of the heating plate away from the second heating element.

[0023] In some embodiments, there are multiple second heating elements, and the multiple second heating elements are spliced ​​around the axis of the heating plate to form an annular structure.

[0024] In some embodiments, a cooling fan is further included, wherein the cooling fan is mounted on the bracket, and an air intake of the cooling fan faces the amplification device.

[0025] In some embodiments, a light-guiding device is provided between the extraction device and the amplification device, and the light-guiding device has a sixth mounting hole and a first light-guiding hole. The sixth mounting hole is arranged corresponding to the third mounting hole. The bottom of the test tube extends into the light-guiding device through the sixth mounting hole, and the light emitted by the fluorescence detection device is irradiated on the bottom of the test tube through the first light-guiding hole.

[0026] In some embodiments, the light guide device includes a light guide disc and a light guide column. The first light guide hole is opened on the side of the light guide disc facing the fluorescence detection device for the light emitted by the fluorescence detection device to pass through. The sixth mounting hole is opened on the side of the light guide disc facing the extraction device. The light guide column is arranged in the light guide disc and is located between the first light guide hole and the sixth mounting hole, and is used to irradiate the light emitted by the fluorescence detection device to the bottom of the test tube through the first light guide hole.

[0027] In some embodiments, a center line of the first light guide hole is perpendicular to a center line of the sixth mounting hole.

[0028] In some embodiments, there are multiple first light guide holes, and the multiple first light guide holes are arranged at intervals around the axis of the optical disk;

[0029] There are a plurality of sixth mounting holes, and the plurality of sixth mounting holes are arranged at intervals around the axis of the optical guide plate, and the first light guide holes are arranged in a one-to-one correspondence with the sixth mounting holes;

[0030] A light guide column is disposed between each of the first light guide holes and the corresponding sixth mounting hole.

[0031] In some embodiments, the optical disc further includes a light guide tube, one end of the light guide tube is connected to the first light guide hole, the other end of the light guide tube is connected to the sixth mounting hole, and the light guide column is disposed in the light guide tube.

[0032] In some embodiments, the fluorescence detection device includes an emission end;

[0033] The diameter of the optical guide plate is smaller than that of the extraction device, which is also smaller than that of the amplification device. A groove structure is defined between the extraction device, the optical guide plate and the amplification device, and the emission end is located in the groove structure.

[0034] In some embodiments, the fluorescence detection device includes a light emitting component, an optical fiber, and a fiber optic transmitter. The light emitting component and the fiber optic transmitter are installed on the bracket. The light emitting component and the fiber optic transmitter are connected through the optical fiber. The fiber optic transmitter has the emitting end. The light emitted by the emitting end is irradiated to the bottom of the test tube through the first light guide hole and the light guide column.

[0035] In some embodiments, there are multiple light emitting components, and the multiple light emitting components are spaced apart around the output shaft of the driving device;

[0036] There are multiple optical fiber transmitters, and the multiple optical fiber transmitters are arranged at intervals around the axis of the bracket.

[0037] In some embodiments, the fluorescence detection device further includes a driving assembly, the light emitting assembly includes a plurality of light sources, a plurality of filters, and a first transmission member, the first transmission member having a transmission end connected to the driving assembly and a mounting end located on the output light path, the plurality of light sources being mounted on the mounting end at intervals around the axis of the mounting end, and a corresponding filter being provided at the front end of the emission head of each light source;

[0038] Whenever the driving assembly drives the first transmission member to rotate once, the first transmission member drives each of the light sources to rotate to the output light path to emit light of corresponding wavelength.

[0039] In some embodiments, the driving assembly includes a second driving member and a conveyor belt, and the first driving member and the second driving member are synchronously driven through the conveyor belt.

[0040] In some embodiments, the driving device includes a third driving member, a synchronous belt and a second transmission member. The third driving member is installed on the bracket, and the second transmission member is installed at the bottom of the amplification device. The third driving member and the second transmission member are synchronously transmitted through the synchronous belt to drive the amplification device to rotate.

[0041] Compared with the prior art, the nucleic acid extraction and amplification integrated detection device according to the embodiment of the present invention has the following advantages:

[0042] The extraction device of an embodiment of the present invention is used to extract nucleic acids from a test tube placed in a placement hole, wherein the first driving member is used to drive the magnetic suction member to rotate around the outer circumference of the test tube placed in the first mounting hole, and the magnetic suction member provided on the outside of the first mounting hole provides a magnetic suction force to adsorb the magnetic beads in the test tube, and drives the magnetic beads to rotate to complete the nucleic acid extraction; the amplification device is used to adjust the temperature of the bottom of the test tube to achieve nucleic acid amplification; the driving device drives the test tube located on the extraction device to rotate relative to the fluorescence detection device, thereby achieving fluorescence detection of the test tube by the fluorescence detection device.

[0043] The present invention integrates the functions of nucleic acid extraction, amplification and fluorescence detection into one, eliminating the need to perform nucleic acid extraction, amplification and fluorescence detection operations in laboratories in different partitions, thereby improving efficiency, shortening detection time, and avoiding the problem of aerosol contamination caused by the need to transport work to different partitions. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] 1 is a schematic structural diagram of a nucleic acid extraction and amplification integrated detection device provided in an embodiment of the present invention;

[0045] FIG2 is a partial exploded view from a first angle of the nucleic acid extraction and amplification integrated detection device provided by an embodiment of the present invention;

[0046] FIG3 is an exploded schematic diagram of a magnetic component and a gear component provided in an embodiment of the present invention;

[0047] FIG4 is an exploded view of a nucleic acid extraction and amplification integrated detection device provided in an embodiment of the present invention;

[0048] 5 is an exploded view of an amplification device, an extraction device, and a light guide device provided by an embodiment of the present invention from a first angle;

[0049] 6 is an exploded view of the amplification device, the extraction device, and the light guide device provided by an embodiment of the present invention from a second angle;

[0050] FIG7 is an exploded schematic diagram of an extraction device provided in an embodiment of the present invention;

[0051] FIG8 is an exploded schematic diagram of a top plate, a gear plate, and a gear member provided in an embodiment of the present invention;

[0052] FIG9 is an exploded view of a top plate, a first heating element, and a heat insulating element provided by an embodiment of the present invention;

[0053] FIG10 is a partial enlarged view of the circled portion A in FIG7 according to an embodiment of the present invention;

[0054] FIG11 is a partial enlarged view of the circled portion B in FIG7 according to an embodiment of the present invention;

[0055] FIG12 is a partial enlarged view of the circled portion C in FIG9 according to an embodiment of the present invention;

[0056] FIG13 is an exploded view of a driving device and an amplifying device provided in an embodiment of the present invention;

[0057] FIG14 is a schematic structural diagram of an amplification device, an extraction device, and a light guide device provided in an embodiment of the present invention;

[0058] FIG15 is a partial exploded view of a bracket, a fluorescence detection device, and a heat dissipation fan provided in an embodiment of the present invention;

[0059] FIG16 is a partial exploded view from a second angle of the integrated nucleic acid extraction and amplification detection device provided in an embodiment of the present invention;

[0060] 17 is an exploded schematic diagram of a guide disc provided in an embodiment of the present invention;

[0061] FIG18 is a partial enlarged view of the circled portion D in FIG2 according to an embodiment of the present invention;

[0062] In the figure, 1, bracket; 11, upper bracket; 12, lower bracket; 2, amplification device; 21, heating plate; 2101, third through hole; 22, second heating element; 23, heat conducting element; 3. Extraction device; 31. Test tube mounting assembly; 3101. First mounting hole; 3102. Gear member; 31021. Second mounting hole; 31022. Fourth mounting hole; 31023. Fifth mounting hole; 31024. Main body; 31025. Gear; 3103. Matching member; 31031. First slide groove; 3104. First heating member; 3105. Heat insulation member; 31051. First base; 31052. First mounting groove; 31053. First connecting member; 310531. Second base; 310532. Second mounting groove; 32. First driving member; 33. Magnetic member; 34. Gear plate; 3401. First through hole; 35. Bottom plate; 3501. Third mounting hole; 3502. Second through hole; 36. Top plate; 3601. Second slide groove; 37. Second connecting member; 4. Fluorescence detection device; 41. Light emitting assembly; 4101. First transmission member; 42. Optical fiber; 43. Optical fiber transmitter; 44. Drive assembly; 4401. Second drive member; 4402. Conveyor belt; 5. Drive device; 51. Third drive member; 52. Synchronous belt; 53. Second transmission member; 6. Cooling fan; 7. Light guide device; 71. Sixth mounting hole; 72. First light guide hole; 73. Light guide disc; 7301. Light guide tube; 73011. Light guide portion; 7302. First disc; 7303. Second disc; 74. Light guide column; 75. Groove structure. DETAILED DESCRIPTION

[0063] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0064] As shown in Figure 1, an embodiment of the present invention provides an all-in-one nucleic acid extraction and amplification detection device for nucleic acid extraction, amplification, and fluorescence detection. In actual use, a test tube is placed on the all-in-one nucleic acid extraction and amplification detection device, and the nucleic acid extraction and amplification detection device extracts, amplifies, and performs fluorescence detection on the nucleic acid solution in the test tube.

[0065] Please refer to Figures 1 to 3 and 5. The nucleic acid extraction and amplification integrated detection device includes a bracket 1, an amplification device 2, an extraction device 3, a fluorescence detection device 4 and a driving device 5. The bracket 1 in this embodiment serves as a mounting structure, which carries the amplification device 2, the extraction device 3, the fluorescence detection device 4 and the driving device 5; the extraction device 3 is used to extract nucleic acids from the nucleic acid solution in the test tube. Magnetic beads are placed in the test tube in this embodiment, and the magnetic beads are fully combined with the nucleic acids after lysis. The extraction device 3 includes a test tube mounting assembly 31, a first driving member 32 and a magnetic member 33. The test tube mounting assembly 31 has a first mounting hole 3101 for placing the test tube (as shown in Figure 3). The magnetic member 33 is arranged on the hole wall of the first mounting hole 3101. The magnetic member 33 provides magnetic attraction to absorb the magnetic beads in the test tube. The first driving member 32 is mounted on the bracket 1 and is used to drive the test tube mounting assembly 31 to rotate, so as to drive the magnetic member 33 to rotate around the outer circumference of the test tube located in the test tube mounting assembly 31. The magnetic beads in the test tube rotate accordingly, so that the nucleic acid is cleaned in the test tube. Purification to complete the extraction of nucleic acid; the extraction device 3 is installed on the amplification device 2, and the amplification device 2 is used to adjust the temperature of the bottom of the test tube to achieve the amplification of the nucleic acid in the test tube; the fluorescence detection device 4 and the driving device 5 in this embodiment are installed on the bracket 1, and the output end of the driving device 5 is connected to the amplification device 2, the amplification device 2 is set on the bracket 1 through the driving device 5, and the driving device 5 is used to drive the amplification device 2 and the extraction device 3 to rotate relative to the fluorescence detection device 4, and emit light to the test tube through the fluorescence detection device 4 to perform fluorescence detection on the nucleic acid in the test tube.

[0066] This embodiment integrates the nucleic acid extraction, amplification and fluorescence detection functions into one, eliminating the need to perform nucleic acid extraction, amplification and fluorescence detection operations in laboratories in different partitions, thereby improving efficiency, shortening detection time, and avoiding the problem of aerosol contamination caused by the need to transport work to different partitions.

[0067] Please refer to Figure 4. The extraction device 3 also includes a gear plate 34, which has a first through hole 3401. The output shaft of the first driving member 32 passes through the amplification device 2 and is installed in conjunction with the first through hole 3401 of the gear plate 34 to achieve the connection between the output shaft of the first driving member 32 and the gear plate 34; the test tube mounting assembly 31 includes a gear member 3102, the gear plate 34 is engaged with the gear member 3102, the gear member 3102 has a first mounting hole 3101, and the magnetic member 33 is arranged on the hole wall of the first mounting hole 3101 of the gear member 3102. The test tube is placed in the first mounting hole 3101. When the first driving member 32 drives the gear plate 34 to rotate to drive the gear member 3102 to rotate, the magnetic beads in the test tube are driven to rotate accordingly.

[0068] As shown in Figure 3, the gear member 3102 includes a cylindrical body 31024 having a first mounting hole 3101 extending through both ends thereof. A toothed portion 31025 is provided at one end of the body 31024 to engage with a gear plate 34, and the toothed portion 31025 is disposed on the circumference of the body 31024. A second mounting hole 31021 is formed in the wall of the first mounting hole 3101. A magnetic member 33 is disposed within the second mounting hole 31021, which utilizes the magnetic attraction force of the magnetic member 33 to attract magnetic beads within a test tube. When the gear plate 34 rotates the body 31024, the position of the magnetic member 33 relative to the test tube within the first mounting hole 3101 also moves with the rotation of the body 31024, thereby causing the magnetic beads within the test tube to rotate, thereby completing the nucleic acid extraction process. Preferably, the magnetic member 33 is a magnet.

[0069] Please refer to Figures 4 to 8. The extraction device 3 also includes a base plate 35 and a top plate 36. A receiving cavity is formed between the base plate 35 and the top plate 36. The gear plate 34 is arranged in the receiving cavity. In this embodiment, a second through hole 3502 is provided on the base plate 35 for the output shaft of the first driving member 32 to pass through and connect with the gear plate 34. The bottom of the gear member 3102 is installed in cooperation with the base plate 35, and the top of the gear member 3102 is installed in cooperation with the top plate 36.

[0070] In this embodiment, a third mounting hole 3501 is provided on the chassis 35 (as shown in FIG10 ) for the bottom of the test tube to pass through. At this time, the portion of the bottom of the test tube passing through the third mounting hole 3501 is exposed to the extraction device 3, and this portion is located above the amplification device 2. The amplification device 2 performs temperature adjustments such as heating and cooling on this portion to achieve nucleic acid amplification.

[0071] It is understood that bolt holes are provided at corresponding positions on the top plate 36 and the bottom plate 35, and the two are connected by bolts. Alternatively, hollow mounting posts can be provided on the bottom plate 35, and bolts can be passed through the bolt holes in the top plate 36 to connect to the mounting posts. The specific connection method is not limited here.

[0072] Referring to Figures 7, 8, and 10, the test tube mounting assembly 31 also includes a mating member 3103, which is mounted on the base plate 35. The gear member 3102 is positioned between the top plate 36 and the mating member 3103, with the gear member 3102 corresponding to the mating member 3103. Furthermore, a fourth mounting hole 31022 is defined on the end surface of the gear member 3102 facing the base plate 35. A ball bearing is positioned within the fourth mounting hole 31022. A first sliding groove 31031 is defined on the side of the mating member 3103 facing the top plate 36. The ball bearing allows the gear member 3102 to rotate within the first sliding groove 31031. When the gear member 3102 rotates, the ball bearing follows the path of the first sliding groove 31031. The provision of the mating member 3103 in this embodiment not only provides support for the installation of the gear member 3102 but also prevents the gear member 3102 from being jerked during rotation. In this embodiment, the third mounting hole 3501 passes through the first sliding groove 31031 for allowing the bottom of the test tube to pass through.

[0073] It can be understood that the gear part 3102 in this embodiment is provided with a fifth mounting hole 31023 on the end face facing the top plate 36, and a ball is provided in the fifth mounting hole 31023. A second slide groove 3601 corresponding to the gear part 3102 is provided on the side of the top plate 36 facing the bottom plate 35. The gear part 3102 can rotate on the second slide groove 3601 through the ball. When the gear part 3102 rotates, the ball rotates along the shape of the second slide groove 3601.

[0074] It should be noted that, in this embodiment, a plurality of fourth mounting holes 31022 are provided at the bottom of the gear part 3102 to balance the installation height between various parts of the gear part 3102 and the mating part 3103; in addition, a plurality of fifth mounting holes 31023 are provided at the top of the gear part 3102 to balance the installation height between various parts of the gear part 3102 and the second slide groove 3601.

[0075] Please refer to Figures 9 and 11. The test tube mounting assembly 31 also includes a first heating element 3104. The first heating element 3104 is arranged on the top plate 36 and is corresponding to the gear element 3102. When the test tube is installed on the test tube mounting assembly 31, the test tube passes through the first heating element 3104, the first mounting hole 3101 and the third mounting hole 3501 in sequence, and the bottom of the test tube is exposed below the extraction device 3, so that the light emitted by the fluorescence detection device 4 can directly hit the bottom of the test tube. The first heating element 3104 is used to heat the top of the test tube to improve the sensitivity of nucleic acid extraction. It can be understood that a through hole is provided on the top plate 36, which is corresponding to the second slide groove 3601 and the first heating element 3104 for the test tube to pass through.

[0076] 9 and 11 , the test tube mounting assembly 31 further includes a heat insulating member 3105 , which is sleeved on the outer circumference of the first heating member 3104 to provide heat insulation and prevent heat from diffusing toward the middle and bottom of the test tube.

[0077] As shown in FIG5 , in some embodiments, there are multiple test tube mounting assemblies 31 , and multiple test tube mounting assemblies 31 are arranged around the circumference of the gear plate 34 to form a test tube mounting group. This embodiment is described using the gear 3102 as an example:

[0078] In this embodiment, the multiple gear parts 3102 are installed on the side of the gear disc 34 in a circular arrangement, and the gear parts 3102 are respectively engaged with the gear disc 34. This method can save installation space. At the same time, this method uses a gear disc 34 to drive multiple gear parts 3102 to rotate synchronously, with fewer transmission times and high efficiency. In addition, there will be no situation where damage to a gear part 3102 affects the operation of the entire nucleic acid extraction and amplification integrated detection device, thereby enhancing the stability of the nucleic acid extraction and amplification integrated detection device. On the other hand, the circular arrangement structure is simpler and more compact, making the heating of the amplification device 2 more concentrated, and achieving more uniform heating. The temperature difference between each test tube is smaller, thereby improving uniformity. Among them, the number of test tube installation groups is n, and the n groups of test tube installation groups are arranged in sequence along the radial direction of the gear disc 34, where n ≥ 1.

[0079] When n is 1, the gear part 3102 is arranged around the circumference of the gear plate 34 to form an annular structure; when n is 2, the gear part 3102 is arranged around the circumference of the gear plate 34 to form an annular structure, which is the inner gear ring; multiple gear parts 3102 are arranged around the outside of the inner gear ring to form another annular structure, which is the outer gear ring. At this time, the outer gear ring is engaged with the inner gear ring, and the rotation of the inner gear ring can be driven by the rotation of the gear plate 34, and the outer gear ring rotates accordingly, realizing the synchronous rotation of the inner gear ring and the outer gear ring.

[0080] It can be understood that the gear elements 3102 of the outer gear ring mesh with two adjacent gear elements 3102 of the inner gear ring, i.e., the gear elements 3102 of the outer gear ring and the gear elements 3102 of the inner gear ring are arranged in an interlaced manner, achieving synchronous transmission. n can also be 3, 4, or more, and the arrangement of the gear elements 3102 is similar. Preferably, in this embodiment, n is 2, and each gear ring has 16 gear elements 3102, which can achieve simultaneous nucleic acid extraction and amplification of 32 samples, improving the efficiency of nucleic acid extraction and amplification, and achieving a single-module multi-throughput configuration.

[0081] Similarly, multiple mating members 3103 are spaced apart around the axis of the bottom plate 35, and the mating members 3103 are arranged in a one-to-one correspondence with the gear members 3102. Multiple second chutes 3601 are spaced apart around the axis of the top plate 36, and the second chutes 3601 are arranged in a one-to-one correspondence with the gear members 3102. Multiple first heating members 3104 are spaced apart around the axis of the top plate 36, and the first heating members 3104 are arranged in a one-to-one correspondence with the gear members 3102. Among them, the thermal insulation members 3105 are arranged corresponding to the first heating members 3104, that is, the multiple thermal insulation members 3105 are spaced apart around the axis of the top plate 36, and the thermal insulation members 3105 are mounted on the outer periphery of the corresponding first heating members 3104, providing thermal insulation and preventing the heat of the first heating members 3104 from dissipating to the middle and bottom of the test tube.

[0082] As shown in Figure 12, the thermal insulation member 3105 in this embodiment includes a first base 31051 connected to the top plate 36 and a first mounting groove 31052 located on the first base 31051. The first base 31051 is a hollow structure, and the aperture of the first mounting groove 31052 is larger than the aperture of the hollow structure, so that when the first heating member 3104 is installed on the thermal insulation member 3105, it will not directly contact the top plate 36, thereby preventing heat from diffusing toward the middle and bottom of the test tube; two adjacent thermal insulation members 3105 are connected by a first connecting member 31053, and the first connecting member 31053 includes a second base 310531 and a second mounting groove 310532. The second base 310531 is connected to the first base 31051, and the second mounting groove 310532 is connected to the first mounting groove 31052, and the second mounting groove 310532 is strip-shaped. It can be understood that the thermal insulation member 3105 and the first connecting member 31053 in this embodiment can be integrally formed or can be composed of an assembled structure; similarly, the two adjacent first heating members 3104 are connected by a second connecting member 37 (as shown in Figure 11). During installation, the first heating member 3104 is installed in the first installation groove 31052, and the second connecting member 37 is installed in the second installation groove 310532.

[0083] It should be noted that the top plate 36 and the bottom plate 35 in this embodiment are preferably disc-shaped structures to match the disc-shaped structure formed by the test tube mounting assembly 31 and the gear plate 34 .

[0084] Please refer to Figures 4, 13 and 14. The amplification device 2 includes a heating disk 21, a second heating element 22 and a heat-conducting element 23. A third through hole 2101 is provided on the heating disk 21 to facilitate the output shaft of the first driving element 32 to pass through the third through hole 2101 of the heating disk 21 and connect with the extraction device 3. The second heating element 22 is installed on the heating disk 21 and is located below the extraction device 3 to heat the bottom of the test tube; the heat-conducting element 23 and the first driving element 32 are arranged on the side of the heating disk 21 away from the second heating element 22. The heat-conducting element 23 is used to conduct heat and cool down to achieve PCR (Polymerase Chain Reaction) amplification.

[0085] In some embodiments, there are multiple second heating elements 22 , and the multiple second heating elements 22 are spliced ​​around the axis of the heating disk 21 to form an annular structure.

[0086] As can be understood, the heating plate 21 is a disc-shaped structure that aligns with the structure of the base plate 35. In this embodiment, the second heating elements 22 are of equal size, and the annular structure formed by the splicing of multiple second heating elements 22 aligns with the annular arrangement of the test tube mounting assembly 31, thereby providing more concentrated heating to the bottom of the test tube. The heating plate 21 in this embodiment has sidewalls extending toward the base plate 35, which can, to a certain extent, prevent heat from dissipating.

[0087] Referring to Figures 15 and 16 , a cooling fan 6 is also included. This cooling fan 6 is mounted on the bracket 1, with its air intake facing the amplification device 2. This fan 6 is used to draw heat out of the device and blow it outward, thereby improving the cooling capacity of the amplification device 2. The cooling fan 6 cooperates with the second heating element 22 and the heat conducting element 23 to achieve PCR amplification. As will be appreciated, a heat dissipation vent is provided on the bracket 1, located below the amplification device 2, for the cooling fan 6 to draw heat away from the heat conducting element 23 through the vent for dissipation.

[0088] In practical applications, one, two, three or more cooling fans 6 may be provided according to actual needs.

[0089] It should be noted that the amplification process in this embodiment is a process of continuous heating and cooling cycles, with about 30-50 heating and cooling cycles. Fluorescence collection is required for each cycle, that is, there are 30-50 amplification cycles and fluorescence collection is also 30-50 times.

[0090] Referring to Figures 2 and 5 , a light guide device 7 is disposed between the extraction device 3 and the amplification device 2. The light guide device 7 has a sixth mounting hole 71 and a first light guide hole 72. The sixth mounting hole 71 corresponds to the third mounting hole 3501, and the bottom of the test tube extends into the light guide device 7 through the sixth mounting hole 71. Light emitted by the fluorescence detection device 4 passes through the light guide device 7 and the first light guide hole 72 and illuminates the bottom of the test tube, thereby achieving fluorescence detection and collection of nucleic acids. It will be appreciated that the light guide device 7 in this embodiment not only serves as a light guide, but also provides a certain degree of light shielding, preventing the influence of external light, as the bottom of the test tube extends into the light guide device 7.

[0091] Please refer to Figures 6 and 17. The light guiding device 7 includes a light guiding disc 73 and a light guiding column 74. The light guiding disc 73 has a first light guiding hole 72 on the side facing the fluorescence detection device 4 for the light emitted by the fluorescence detection device 4 to pass through. The light guiding disc 73 has a sixth mounting hole 71 on the side facing the extraction device 3. The light guiding column 74 is arranged in the light guiding disc 73 and is located between the first light guiding hole 72 and the sixth mounting hole 71. It is used to irradiate the light emitted by the fluorescence detection device 4 through the first light guiding hole 72 to the bottom of the test tube.

[0092] Referring to Figure 6 , in this embodiment, the optical guide plate 73 includes a first plate 7302 and a second plate 7303, which are connected to form a mounting cavity. The first plate 7302 has a support wall extending toward the second plate 7303. This support wall defines a first light guide hole 72, and a light guide column 74 is mounted on the second plate 7303. When the fluorescence detection device 4 begins operation, light emitted by the device enters through the first light guide hole 72 and is transmitted by the light guide column 74 directly onto the bottom of the test tube, thereby collecting fluorescence light and feeding the collected information back to the fluorescence detection device 4, thereby achieving fluorescence detection of nucleic acids.

[0093] In some embodiments, the first light guide hole 72 and the light guide column 74 form a light path for the light emitted by the fluorescence detection device 4 to pass through. The center line of the first light guide hole 72 is set at a certain angle to the center line of the sixth mounting hole 71 so that the light incident from the side can be irradiated to the bottom of the test tube; preferably, the center line of the first light guide hole 72 is set perpendicular to the center line of the sixth mounting hole 71.

[0094] As shown in Figures 4 and 5, the optical disk 73 also includes a light guide tube 7301, which is arranged on the second disk 7303, and the end of the light guide tube 7301 close to the first light guide hole 72 has a second light guide hole, and the second light guide hole is connected to the first light guide hole 72. The end of the light guide tube 7301 close to the sixth mounting hole 71 has a light guide part 73011 (as shown in Figure 17), which extends into the sixth mounting hole 71, and the light guide part 73011 has a through hole to achieve communication with the sixth mounting hole 71. The light guide column 74 is arranged in the light guide tube 7301, and the light emitted by the fluorescence detection device 4 is focused in the light guide tube 7301, and the light is directly irradiated to the bottom of the test tube through the light guide column 74, thereby improving the detection accuracy.

[0095] As shown in FIG17 , in some embodiments, a plurality of first light guide holes 72 are provided, and the plurality of first light guide holes 72 are spaced apart on the support wall around the axis of the guide plate 73; a plurality of sixth mounting holes 71 are provided, and the plurality of sixth mounting holes 71 are spaced apart around the axis of the guide plate 73. It will be appreciated that in this embodiment, the plurality of sixth mounting holes 71 are arranged in a circular pattern on the first plate 7302, corresponding to the positions of the mating members 3103, so that one sixth mounting hole 71 corresponds to one third mounting hole 3501, thereby enabling fluorescence detection of nucleic acids in a test tube. In this case, the distances between the sixth mounting holes 71 and the center of the first plate 7302 vary. The sixth mounting hole 71 corresponding to the third mounting hole 3501 on the inner circle is defined as R1, and is closer to the center of the first plate 7302. The sixth mounting hole 71 corresponding to the third mounting hole 3501 on the outer circle is defined as R2, and is farther from the center of the first plate 7302. R1 and R2 are arranged in an intersecting manner, i.e., an R2 is provided between every two adjacent R1s, corresponding one-to-one with the position of each third mounting hole 3501. Of course, in some embodiments, if the mating element 3103 has only one circle, the distances between the sixth mounting holes 71 and the center of the first plate 7302 are all equal.

[0096] It should be noted that the first light guide holes 72 and the sixth mounting holes 71 are arranged in a one-to-one correspondence, and a light guide column 74 is disposed between each first light guide hole 72 and the corresponding sixth mounting hole 71 .

[0097] In some embodiments, the fluorescence detection device 4 includes an emitting end; as shown in Figure 18, the diameter of the guide light disc 73 is smaller than the diameter of the extraction device 3, and the diameter of the guide light disc 73 is smaller than the diameter of the amplification device 2. A groove structure 75 (as shown in Figure 14) is defined between the extraction device 3, the guide light disc 73 and the amplification device 2. The emitting end is located in the groove structure 75. In the process of the emitting end emitting light, the groove structure 75 can achieve a light shielding effect, so that the light path will not be disturbed by external light, preventing external light sources from having a large impact on the light receiving and emitting of the fluorescence detection device 4, avoiding affecting the detection results, and making the detection structure more accurate.

[0098] Referring to FIG. 2 , the fluorescence detection device 4 includes a light emitting assembly 41, an optical fiber 42, and a fiber optic transmitter 43. The light emitting assembly 41 is mounted on the bracket 1, and the fiber optic transmitter 43 is mounted on the bracket 1 via a mounting base. The fiber optic transmitter 43 has a transmitting end, which also serves as a receiving end for receiving the detection results fed back from the test tube. The light emitting assembly 41 and the fiber optic transmitter 43 are connected by two optical fibers 42. One optical fiber 42 is used to connect the transmitting end of the light emitting assembly 41 and the transmitting end of the fiber optic transmitter 43, and the other optical fiber 42 is used to connect the receiving end of the light emitting assembly 41 and the receiving end of the fiber optic transmitter 43. Light emitted from the transmitting end of the fiber optic transmitter 43 is irradiated onto the bottom of the test tube through the first light guide hole 72 and the light guide column 74.

[0099] As shown in Figure 2, in some embodiments, there are multiple light emitting components 41, and the multiple light emitting components 41 are arranged at intervals around the output shaft of the driving device 5; there are multiple optical fiber transmitters 43, and the multiple optical fiber transmitters 43 are arranged at intervals around the axis of the bracket 1.

[0100] In this embodiment, there are four optical transmission components 41, which are located at the four corners of the bracket 1. Similarly, there are four optical fiber transmitters 43, and the optical fiber transmitters 43 and the optical transmission components 41 are staggered, that is, a optical fiber transmitter 43 is provided between two adjacent optical transmission components 41. By using the intervals between the optical transmission components 41 to set the optical fiber transmitter 43, the structure can be set more compact without occupying more space.

[0101] As shown in Figure 2, the fluorescence detection device 4 also includes a driving component 44, the light emitting component 41 includes multiple light sources, multiple filters and a first transmission member 4101, the first transmission member 4101 has a transmission end connected to the driving component 44 and a mounting end located on the output light path, multiple light sources are installed on the mounting end at intervals around the axis of the mounting end, and a filter is correspondingly provided at the front end of the emission head of each light source.

[0102] As can be understood, the light emitting assembly 41 in this embodiment incorporates different filters. Each light source works in pairs with a corresponding filter to achieve the effect of outputting and receiving light in a preset wavelength band. Five filters are preferably present, allowing the light source to emit light in five different wavelength bands through the filters. The mounting end of the first transmission member 4101 in this embodiment has a fan-shaped structure, with five light sources arranged sequentially along the mounting end. The drive assembly 44 drives the first transmission member 4101 to rotate. Each time the drive assembly 44 rotates the first transmission member 4101, it drives a light source onto the output light path to emit light of the corresponding wavelength. The light source emits monochromatic light through the filters. This monochromatic light is transmitted through the optical fiber 42 to the fiber optic transmitter 43, and then illuminates the bottom of the test tube through the first light guide hole 72 and the light guide column 74 for fluorescence collection. The drive assembly 44 drives the first transmission member 4101 to switch between different light sources and filters, sequentially generating light of different colors, until the five fluorescence collections from the test tube are completed.

[0103] It is understandable that the light source in this embodiment can be laser or other types of light sources, which are not particularly limited here, as long as they can be matched with corresponding filters in pairs to achieve the effect of outputting and receiving light in a preset band.

[0104] As shown in FIG2 , in some embodiments, the drive assembly 44 includes a second drive member 4401 and a conveyor belt 4402. The first transmission member 4101 and the second drive member 4401 are synchronously driven by the conveyor belt 4402. In this embodiment, the conveyor belt 4402 is sequentially connected to the drive ends of multiple first transmission members 4101. The second drive member 4401 drives the multiple first transmission members 4101, thereby achieving simultaneous switching of multiple light sources to generate different monochromatic lights, thereby improving efficiency.

[0105] As shown in Figure 2, the drive device 5 includes a third drive member 51, a timing belt 52, and a second transmission member 53. The third drive member 51 is mounted on the bracket 1, and the second transmission member 53 is mounted on the bottom of the amplification device 2. The third drive member 51 and the second transmission member 53 are synchronously driven by the timing belt 52 to drive the amplification device 2 to rotate. It can be understood that the second transmission member 53 is sleeved on the output shaft of the first drive member 32, and the two do not contact each other.

[0106] After completing the five fluorescence collections of the test tube, the third driving member 51 drives the synchronous belt 52 to drive the second transmission member 53 to rotate, thereby driving the amplification device 2 and the light guide device 7 located on the amplification device 2 to rotate, so that the next first light guide hole 72 is aligned with the emitting end of the optical fiber transmitter 43, and the above-mentioned five-color fluorescence collection is performed again. The second transmission member 53 drives the light guide device 7 to rotate 8 times in sequence, and a total rotation of about 90° can complete the fluorescence collection of the bottom of all test tubes on the extraction device 3.

[0107] The device provided in this embodiment can simultaneously place 32 samples into the device and output the results together. The elimination of manual testing improves the efficiency of testing and shortens the testing time. At the same time, the structure realizes single-module multi-throughput. Standard control and blank control under the same conditions can be added to the 32 samples, which can meet the use requirements, further improve the efficiency and accuracy of the device, and also improve the detection throughput as a screening reagent.

[0108] Referring to Figure 15 together, the bracket 1 includes an upper bracket 11 and a lower bracket 12, and the upper bracket 11 and the lower bracket 12 are connected by fasteners. The upper bracket 11 and the lower bracket 12 serve as a bearing platform to provide installation space for the installation of the amplification device 2, the extraction device 3, the fluorescence detection device 4, the drive device 5 and the cooling fan 6. A space is formed between the upper bracket 11 and the lower bracket 12, and the first drive member 32 and the third drive member 51 are located in the space. The first drive member 32, the third drive member 51, the amplification device 2, and the fluorescence detection device 4 are installed on the upper bracket 11, and the cooling fan 6 is installed on the lower bracket 12.

[0109] It is understood that bolt holes are provided at corresponding positions on the upper bracket 11 and the lower bracket 12, and the two are connected by bolts. Alternatively, a hollow mounting post can be provided on the lower bracket 12, and bolts can be passed through the bolt holes in the upper bracket 11 to connect to the mounting post. The specific connection method is not limited here.

[0110] In summary, the embodiments of the present invention provide an all-in-one nucleic acid extraction, amplification and detection device, which is small in size and easy to carry and transport. It not only reduces the difficulty of laboratory construction and operation, but also solves the problem of aerosol pollution easily generated in the laboratory, improves safety, and at the same time can realize a single device product to complete the steps of nucleic acid extraction, amplification, and detection, integrating multiple functions into one.

[0111] It should be understood that the terms "first", "second", etc. are used in the present invention to describe various information, but such information should not be limited to these terms. These terms are only used to distinguish information of the same type from each other. For example, without departing from the scope of the present invention, "first" information may also be referred to as "second" information, and similarly, "second" information may also be referred to as "first" information. In addition, the directions or positional relationships indicated by the terms "up", "down", "left", "right", etc. are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operate in a specific direction, and therefore should not be understood as limiting the present invention.

[0112] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention. These improvements and substitutions should also be regarded as the scope of protection of the present invention.

Claims

1. A nucleic acid extraction and amplification integrated detection device, characterized in that: include: Bracket (1); an amplification device (2), arranged on the support (1) and used for adjusting the temperature; An extraction device (3) is mounted on the amplification device (2), the extraction device (3) comprising a test tube mounting assembly (31), a first driving member (32) and a magnetic member (33), the test tube mounting assembly (31) having a first mounting hole (3101), the magnetic member (33) being arranged on the hole wall of the first mounting hole (3101), the first driving member (32) being mounted on the bracket (1) and used for driving the test tube mounting assembly (31) to rotate, thereby driving the magnetic member (33) to rotate around the outer circumference of the test tube located in the test tube mounting assembly (31) and extracting nucleic acid; A fluorescence detection device (4), mounted on the support (1), and used for performing fluorescence detection on the test tube; as well as A driving device (5) is mounted on the support (1); an output end of the driving device (5) is connected to the amplification device (2) and is used to drive the amplification device (2) and the extraction device (3) to rotate relative to the fluorescence detection device (4).

2. The nucleic acid extraction and amplification integrated detection device according to claim 1, characterized in that: The extraction device (3) further comprises a gear plate (34), and the output shaft of the first driving member (32) passes through the amplification device (2) and is connected to the gear plate (34); The test tube mounting assembly (31) comprises a gear member (3102), the gear member (3102) has the first mounting hole (3101), the gear plate (34) is meshed with the gear member (3102), and the magnetic attraction member (33) is arranged on the gear member (3102).

3. The nucleic acid extraction and amplification integrated detection device according to claim 2, characterized in that: The first mounting hole (3101) passes through both ends of the gear component (3102); a second mounting hole (31021) is provided on the hole wall of the first mounting hole (3101); and the magnetic attraction component (33) is arranged in the second mounting hole (31021).

4. The nucleic acid extraction and amplification integrated detection device according to claim 2, characterized in that: The extraction device (3) further comprises a bottom plate (35) and a top plate (36), a receiving cavity being formed between the bottom plate (35) and the top plate (36), the gear plate (34) being arranged in the receiving cavity, the bottom plate (35) being arranged on the amplification device (2), and a third mounting hole (3501) for the bottom of the test tube to pass through being arranged on the bottom plate (355).

5. The nucleic acid extraction and amplification integrated detection device according to claim 4, characterized in that: The end surface of the gear member (3102) facing the bottom plate (35) is provided with a fourth mounting hole (31022), and the end surface of the gear member (3102) facing the top plate (36) is provided with a fifth mounting hole (31023), and balls are provided in both the fourth mounting hole (31022) and the fifth mounting hole (31023); The test tube mounting assembly (31) further comprises a matching piece (3103), wherein the matching piece (3103) is arranged on the bottom plate (35), and a first slide groove (31031) is provided on a side of the matching piece (3103) facing the top plate (36), and the third mounting hole (3501) passes through the first slide groove (31031); a second slide groove (3601) corresponding to the gear piece (3102) is provided on a side of the top plate (36) facing the bottom plate (35); The gear component (3102) is arranged between the top plate (36) and the matching component (3103), the gear component (3102) and the matching component (3103) are arranged correspondingly, and the gear component (3102) can rotate on the first slide groove (31031) and the second slide groove (3601) through the ball bearing.

6. The nucleic acid extraction and amplification integrated detection device according to claim 4, characterized in that: The test tube mounting assembly (31) further comprises a first heating element (3104), wherein the first heating element (3104) is disposed on the top plate (36) and is arranged corresponding to the gear element (3102).

7. The nucleic acid extraction and amplification integrated detection device according to claim 6, characterized in that: The test tube installation assembly (31) further comprises a heat insulating member (3105), wherein the heat insulating member (3105) is sleeved on the outer peripheral side of the first heating member (3104).

8. The nucleic acid extraction and amplification integrated detection device according to any one of claims 2 to 7, characterized in that: There are multiple test tube installation components (31), and the multiple test tube installation components (31) are arranged around the circumference of the gear plate (34) to form a test tube installation group; The number of the test tube installation groups is n, and the n test tube installation groups are arranged in sequence along the radial direction of the gear plate (34), wherein n≥1.

9. The nucleic acid extraction and amplification integrated detection device according to claim 1, characterized in that: The amplification device (2) comprises a heating plate (21), a second heating element (22) and a heat-conducting element (23); the second heating element (22) is arranged on the heating plate (21) and is located below the extraction device (3); the heat-conducting element (23) and the first driving element (32) are arranged on a side of the heating plate (21) away from the second heating element (22).

10. The nucleic acid extraction and amplification integrated detection device according to claim 9, characterized in that: There are multiple second heating elements (22), and the multiple second heating elements (22) are spliced ​​around the axis of the heating plate (21) to form an annular structure.

11. The nucleic acid extraction and amplification integrated detection device according to claim 1, characterized in that: It also includes a cooling fan (6), which is installed on the bracket (1), and the air intake of the cooling fan (6) faces the amplification device (2).

12. The nucleic acid extraction and amplification integrated detection device according to claim 4, characterized in that: A light guide device (7) is arranged between the extraction device (3) and the amplification device (2), and the light guide device (7) has a sixth mounting hole (71) and a first light guide hole (72). The sixth mounting hole (71) is arranged corresponding to the third mounting hole (3501), and the bottom of the test tube extends into the light guide device (7) through the sixth mounting hole (71), and the light emitted by the fluorescence detection device (4) is irradiated onto the bottom of the test tube through the first light guide hole (72).

13. The nucleic acid extraction and amplification integrated detection device according to claim 12, characterized in that: The light guide device (7) comprises a light guide disc (73) and a light guide column (74); the light guide disc (73) is provided with the first light guide hole (72) on the side facing the fluorescence detection device (4) for light emitted by the fluorescence detection device (4) to pass through; the light guide disc (73) is provided with the sixth mounting hole (71) on the side facing the extraction device (3); the light guide column (74) is arranged in the light guide disc (73) and is located between the first light guide hole (72) and the sixth mounting hole (71) and is used for irradiating the light emitted by the fluorescence detection device (4) to the bottom of the test tube through the first light guide hole (72).

14. The nucleic acid extraction and amplification integrated detection device according to claim 13, characterized in that: The center line of the first light guide hole (72) is arranged perpendicular to the center line of the sixth mounting hole (71).

15. The nucleic acid extraction and amplification integrated detection device according to claim 14, characterized in that: There are a plurality of the first light guide holes (72), and the plurality of the first light guide holes (72) are arranged at intervals around the axis of the optical disk (73); There are a plurality of the sixth mounting holes (71), the plurality of the sixth mounting holes (71) are arranged at intervals around the axis of the optical guide disc (73), and the first light guide holes (72) and the sixth mounting holes (71) are arranged in a one-to-one correspondence; A light guide column (74) is disposed between each of the first light guide holes (72) and the corresponding sixth mounting hole (71).

16. The nucleic acid extraction and amplification integrated detection device according to any one of claims 13 to 15, characterized in that: The optical disc (73) further comprises a light guide tube (7301), one end of the light guide tube (7301) being connected to the first light guide hole (72), the other end of the light guide tube (7301) being connected to the sixth mounting hole (71), and the light guide column (74) being arranged in the light guide tube (7301).

17. The nucleic acid extraction and amplification integrated detection device according to claim 13, characterized in that: The fluorescence detection device (4) comprises an emission end; The diameter of the optical guide disc (73) is smaller than the diameter of the extraction device (3), and the diameter of the optical guide disc (73) is smaller than the diameter of the amplification device (2). A groove structure (75) is defined between the extraction device (3), the optical guide disc (73) and the amplification device (2), and the emission end is located in the groove structure (75).

18. The nucleic acid extraction and amplification integrated detection device according to claim 17, characterized in that: The fluorescence detection device (4) comprises a light emitting component (41), an optical fiber (42) and an optical fiber transmitter (43); the light emitting component (41) and the optical fiber transmitter (43) are mounted on the bracket (1); the light emitting component (41) and the optical fiber transmitter (43) are connected via the optical fiber (42); the optical fiber transmitter (43) has an emitting end; light emitted by the emitting end is irradiated onto the bottom of the test tube via the first light guide hole (72) and the light guide column (74).

19. The nucleic acid extraction and amplification integrated detection device according to claim 18, characterized in that: There are a plurality of light emitting components (41), and the plurality of light emitting components (41) are arranged at intervals around the output shaft of the driving device (5); There are a plurality of optical fiber transmitters (43), and the plurality of optical fiber transmitters (43) are arranged at intervals around the axis of the bracket (1).

20. The nucleic acid extraction and amplification integrated detection device according to claim 18 or 19, characterized in that: The fluorescence detection device (4) further comprises a driving component (44), the light emitting component (41) comprises a plurality of light sources, a plurality of filters and a first transmission member (4101), the first transmission member (4101) comprising a transmission end connected to the driving component (44) and a mounting end located on the output light path, the plurality of light sources being mounted on the mounting end at intervals around the axis of the mounting end, and a corresponding filter being arranged at the front end of the emitting head of each light source; Whenever the driving component (44) drives the first transmission member (4101) to rotate once, the first transmission member (4101) drives one of the light sources to rotate to the output light path to emit light of a corresponding wavelength.

21. The nucleic acid extraction and amplification integrated detection device according to claim 20, characterized in that: The driving assembly (44) includes a second driving member (4401) and a conveyor belt (4402), and the first transmission member (4101) and the second driving member (4401) are synchronously driven via the conveyor belt (4402).

22. The nucleic acid extraction and amplification integrated detection device according to claim 1, characterized in that: The driving device (5) comprises a third driving member (51), a synchronous belt (52) and a second transmission member (53); the third driving member (51) is mounted on the bracket (1); the second transmission member (53) is mounted on the bottom of the amplification device (2); the third driving member (51) and the second transmission member (53) are synchronously driven by the synchronous belt (52) to drive the amplification device (2) to rotate.

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