Universal detection test tube based on micro-magnetic control technology and detection method

Abstract

The present invention relates to the technical field of detection. Disclosed are a universal detection test tube based on micro-magnetic control technology and a detection method. The universal detection test tube comprises a micro-magnetic control tube and a sealed transmission mechanism, the sealed transmission mechanism being arranged at the top of the micro-magnetic control tube, and a reaction chamber being arranged at the bottom of the micro-magnetic control tube. The sealed transmission mechanism comprises a top cover, and the top cover is arranged at the top of the micro-magnetic control tube. The top cover is provided with a first layer of threads used for being connected to an inner sealing recess and a second layer of threads used for being connected to a tube opening of the micro-magnetic control tube, and a sealed transmission shaft is arranged within the top cover. A top clamping head is arranged at an upper end of the sealed transmission shaft, and a supporting ring having an inner diameter larger than that of the sealed transmission shaft is arranged below the sealed transmission shaft. A bottom clamping head having an inner diameter smaller than that of the supporting ring is arranged below the supporting ring. The sealed transmission shaft, the supporting ring, the top clamping head, and the bottom clamping head are integrally formed as a single unit. The sealing function and the stirring function of the test tube are achieved by means of the sealed transmission mechanism.

Description

A universal detection test tube and detection method based on micromagnetic control technology Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a universal detection test tube and a detection method based on micromagnetic control technology. Background Art

[0002] The principle of nucleic acid extraction using magnetic beads is similar to that of silica membrane centrifuge columns. Using nanotechnology, the surface of superparamagnetic nanoparticles is modified and enhanced to produce superparamagnetic silica nanoparticles. These magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules at a microscopic interface. Leveraging the superparamagnetic properties of silica-coated nanoparticles, under the influence of chaotropic salts (such as guanidine hydrochloride and guanidine isothiocyanate) and an external magnetic field, DNA and RNA can be isolated from samples such as blood, animal tissue, food, and pathogenic microorganisms. This method has applications in a variety of fields, including clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, and molecular biology research.

[0003] In modern medical testing, the testing items of various samples (such as sputum, blood, cerebrospinal fluid, pleural effusion, body fluids, pathological sections, etc.) all need to undergo pre-separation, mixing, stirring, temperature adjustment, cleaning, testing and other functions. However, there is no universal test tube that can realize the function of processing multiple different samples for different tests based on existing technologies and methods.

[0004] For example, nucleic acid amplification detection technology is a process that provides conditions for in vitro amplification of nucleic acid fragments, causing them to amplify exponentially and in large quantities, adds fluorescent dyes or fluorescent markers during the nucleic acid amplification process, uses optical devices to detect the strength of the fluorescent signal, and obtains the nucleic acid amplification results by processing and analyzing the fluorescent signal.

[0005] However, the nucleic acid extraction and amplification test tubes in the related art have the problem of poor sealing, which easily causes contamination of the sample nucleic acid. Moreover, the nucleic acid extraction and amplification test in the related art have the problem of insufficient reflection.

[0006] Moreover, in the related technology, nucleic acid extraction and nucleic acid amplification are two steps, and the normal existing process is separate. It requires two sets of equipment, reagent tubes and consumables: nucleic acid extraction test tubes plus nucleic acid extraction instrument and nucleic acid amplification test tubes plus nucleic acid amplification instrument.

[0007] Summary of the Invention

[0008] The present invention provides a universal detection test tube and a detection method based on micro-magnetic control technology, which solves the above-mentioned technical problems through a sealed transmission mechanism.

[0009] According to one aspect of the present disclosure, a universal detection test tube based on micromagnetic control technology is provided, comprising a micromagnetic control tube and a sealing transmission mechanism, wherein the sealing transmission mechanism is provided at the top of the micromagnetic control tube and a reaction chamber is provided at the bottom of the micromagnetic control tube;

[0010] The sealing transmission mechanism includes a top cover, which is arranged on the top of the micromagnetic control tube. The top cover is provided with a first layer of threads for connecting to the inner sealing groove and a second layer of threads for connecting to the pipe mouth of the micromagnetic control tube. A sealing transmission shaft is arranged in the top cover, and a top clamping joint is arranged at the upper end of the sealing transmission shaft. A support ring with an inner diameter larger than the sealing transmission shaft is arranged below the sealing transmission shaft. A bottom clamping joint with an inner diameter smaller than the support ring is arranged below the support ring. The sealing transmission shaft, support ring, top clamping joint and bottom clamping joint are integrated into one unit.

[0011] A liquid injection and stirring mechanism is provided below the sealing transmission mechanism, and the liquid injection and stirring mechanism is used to perform liquid injection and stirring. A pre-setting mechanism is provided outside the liquid injection and stirring mechanism, and the pre-setting mechanism is used to pre-store solids or liquids used for the reaction;

[0012] A sealing liquid injection mechanism and a reaction mechanism are provided below the liquid injection and stirring mechanism.

[0013] The sealed liquid injection mechanism is used to isolate the reagent in the micromagnetic control tube from the reagent in the reaction mechanism; the reaction mechanism is used to clean the nucleic acid to be detected; and the reaction chamber is used to react the nucleic acid with the reaction liquid.

[0014] In one possible implementation, a first circular through hole is provided in the middle of the top cover, the first through hole has a horizontally opened circular groove, the support ring is movably connected to the circular groove, and the support ring rotates in the circular groove; a second circular through hole is provided in the middle of the inner sealing groove, and the inner sealing groove is provided with a thread for connecting to the first layer of thread of the top cover.

[0015] In a possible implementation, the top clamping joint is connected to an external motor drive mechanism and rotates under the drive of the motor drive mechanism.

[0016] In one possible implementation, the liquid injection and stirring mechanism includes a liquid injection hollow rod, a top clamping groove and a double-layer stirring blade. The upper end of the liquid injection hollow rod is clamped to the bottom clamping joint, and the double-layer stirring blade is spirally fixedly connected to the lower end of the liquid injection hollow rod. The double-layer stirring blade is located above the sealed liquid injection mechanism.

[0017] In a possible implementation, the preset mechanism includes a preset cover and a preset groove, the preset cover has a circular fourth through hole in the middle, the preset groove has a cylindrical groove in the middle, and the sealing transmission shaft passes through the cylindrical groove and the fourth through hole.

[0018] In a possible implementation, the pre-groove is divided into multiple grooves by partitions in the vertical direction, the bottom of the pre-groove cover is provided with multiple slots to adapt to the positions of the multiple partitions in the pre-groove, and the bottom of the pre-groove is designed with a sloped opening.

[0019] In one possible implementation, the sealed liquid injection mechanism includes a sealing plate and an injection plate, the injection plate is provided with a plurality of circular holes connected to the various cavities of the reaction mechanism, and the lower side of the sealing plate is provided with a plurality of cylindrical protrusions corresponding to the circular holes, and the protrusions are used to seal the circular holes.

[0020] In one possible implementation, the reaction mechanism includes a circumferentially arranged first connecting cavity, a first isolation cavity, a cleaning cavity, a second isolation cavity, and a second connecting cavity, wherein the second connecting cavity is connected to the reaction cavity, and the reaction cavity is a cone that is wide at the top and narrow at the bottom.

[0021] According to one aspect of the present disclosure, a method for detecting nucleic acid amplification by stirring and extraction is provided. The method is applied to a universal detection test tube based on micromagnetic control technology, and the method comprises:

[0022] Open the lid of the micromagnetic control tube and add the sample into the space between the preset mechanism and the sealed liquid injection mechanism in the micromagnetic control tube;

[0023] Sealing the micro-magnetic control tube through the top cover, the sealing transmission shaft and the inner sealing groove;

[0024] Rotate the sealing transmission shaft manually or by motor drive;

[0025] The magnetic bead mixed liquid is mixed with the test sample in the pre-set groove, and the paraffin at the bottom of the pre-set groove is melted by heating, and the liquid at the bottom of the pre-set groove flows into the middle of the micromagnetic control tube;

[0026] The micro-magnetic control tube is inserted into the shaftless ball gear, and the test sample begins to heat up and lyse. The external magnet rotates repeatedly to drive the magnetic beads inside the micro-magnetic control tube to move. The lysed nucleic acid combines with the magnetic beads. Under the action of the external magnetic beads, the magnetic beads pass through the first connecting chamber, the first isolation chamber, the cleaning chamber, the second isolation chamber, and the second connecting chamber in sequence to complete the cleaning.

[0027] The paraffin on the top of the reaction chamber is melted by temperature control, and the magnetic beads carrying nucleic acids enter the reaction chamber;

[0028] Under the action of the reagents inside the reaction chamber, the magnetic beads are separated from the nucleic acid and fall to the bottom of the reaction chamber. The nucleic acid reacts with the reaction liquid in the reaction chamber, and the detection instrument collects fluorescence from the side of the reaction chamber.

[0029] In one possible implementation, the method further includes performing the following steps before performing the detection:

[0030] Add the reaction solution, paraffin wax and biological reagent into the reaction chamber in sequence;

[0031] Adding silicone oil into the first and second isolation chambers of the reaction mechanism, and adding cleaning liquid into the cleaning chamber;

[0032] Solidified paraffin is pre-placed on the bottom of each tank of the pre-set tank;

[0033] Add magnetic bead mixed liquid onto the paraffin at the bottom of one of the pre-set tanks.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] The disclosed embodiments of a universal test tube and detection method based on micromagnetic control technology conduct reactions within the micromagnetic control tube, with a spiral seal at the top for enhanced effectiveness. The sealing transmission mechanism provides both sealing and stirring functions, reducing the risk of sample nucleic acid contamination and ensuring sufficient reaction progress through stirring. Paraffin wax is injected into the inner wall of the inner sealing groove, providing a secondary seal and further enhancing the sealing effect.

[0036] Moreover, the hollow injection rod of the injection and stirring mechanism can facilitate the addition of sample liquid into the interior of the micromagnetic control tube, and the double-layer stirring blades can stir the reaction liquid more evenly.

[0037] The preset slots in the preset mechanism can be of any number, and their function is to place various reagents and substances in advance and to isolate them before use. The function of the sealed injection hole mechanism is to facilitate the injection of various reagents and substances into the reaction tank and to seal it; the various slots inside the reaction tank can be injected with different liquids, and can be moved between different liquids under the action of the magnetic beads to achieve the desired effect. Finally, the required substances can move together with the magnetic beads inside the reaction chamber for mixing; under specific conditions inside the reaction chamber, a series of specific reactions occur in the internal substances. The inner bottom of the preset slot adopts a sloped opening design to facilitate the flow of reagents out of the preset slot to the inside of the micromagnetic control tube. Through the cooperation of the preset mechanism and the reaction mechanism, the function of processing a variety of different samples for different tests can be further realized.

[0038] A universal detection test tube based on micromagnetic control technology in an embodiment of the present disclosure combines two steps using a magnetic bead method, saving operation steps, time and space, and also saving costs. The nucleic acid is transferred in a sealed environment without causing aerosol contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] FIG1 shows an anatomical diagram of a universal detection test tube based on micromagnetic control technology from a first perspective according to an embodiment of the present disclosure.

[0040] FIG2 shows an anatomical diagram of a universal detection test tube based on micromagnetic control technology from a second perspective according to an embodiment of the present disclosure.

[0041] FIG3 shows a partial anatomical diagram of a universal detection test tube based on micromagnetic control technology according to an embodiment of the present disclosure.

[0042] FIG4 is a schematic diagram showing a sealing and liquid injection mechanism, a reaction mechanism, and a reaction chamber according to an embodiment of the present disclosure.

[0043] FIG5 shows an exploded view of a universal detection test tube based on micro-magnetic control technology according to an embodiment of the present disclosure.

[0044] FIG6 shows a schematic diagram of a universal detection test tube based on micro-magnetic control technology according to an embodiment of the present disclosure.

[0045] FIG7 is a schematic diagram showing the partial anatomy of a preset mechanism according to an embodiment of the present disclosure.

[0046] In the figure: 1. micromagnetic control tube; 2. sealed transmission mechanism; 21. top cover; 22. sealed transmission shaft; 23. support ring; 24. top clamping joint; 25. bottom clamping joint; 26. inner sealing groove; 3. liquid injection and stirring mechanism; 31. liquid injection hollow rod; 32. top clamping groove; 33. double-layer stirring blade; 4. presetting mechanism; 41. presetting cover; 42. presetting groove; 5. sealed liquid injection mechanism; 51. sealing plate; 52. liquid injection plate; 6. reaction mechanism; 61. first connecting chamber; 62. first isolation chamber; 63. cleaning chamber; 64. second isolation chamber; 65. second connecting chamber; 7. reaction chamber. DETAILED DESCRIPTION

[0047] Various exemplary embodiments, features, and aspects of the present disclosure will be described in detail below with reference to the accompanying drawings. The same reference numerals in the accompanying drawings represent elements with the same or similar functions. Although various aspects of the embodiments are shown in the accompanying drawings, the drawings are not necessarily drawn to scale unless otherwise indicated.

[0048] The word “exemplary” is used exclusively herein to mean “serving as an example, example, or illustration.” Any embodiment described herein as “exemplary” is not necessarily to be construed as preferred or advantageous over other embodiments.

[0049] In addition, numerous specific details are provided in the following detailed description to better illustrate the present disclosure. Those skilled in the art will appreciate that the present disclosure can be practiced without certain specific details. In some instances, methods, means, components, and circuits well known to those skilled in the art are not described in detail in order to highlight the main points of the present disclosure.

[0050] 1-7 , a universal detection test tube based on micromagnetic control technology disclosed herein includes a micromagnetic control tube 1 , a sealing transmission mechanism 2 , a liquid injection and stirring mechanism 3 , a presetting mechanism 4 , a sealing liquid injection mechanism 5 , a reaction mechanism 6 , and a reaction chamber 7 ;

[0051] Among them, the sealed transmission mechanism 2 includes a top cover 21, a sealed transmission shaft 22, a support ring 23, a top clamping joint 24, a bottom clamping joint 25, and an inner sealing groove 26. The top cover 21 is provided with a first layer of threads for connecting to the inner sealing groove 26 and a second layer of threads for connecting to the nozzle of the micromagnetic control tube 1. The top clamping joint 24 is provided at the upper end of the sealed transmission shaft 22. The support ring 23 is located in the middle of the sealed transmission shaft 22, and the middle refers to the position of the two ends of the non-sealed transmission shaft 22. The inner diameter of the support ring 23 is larger than the inner diameter of the sealed transmission shaft 22. The sealed transmission shaft 22, the support ring 23, the top clamping joint 24 and the bottom clamping joint 25 are a whole.

[0052] The liquid injection and stirring mechanism 3 includes a liquid injection hollow rod 31, a top clamping groove 32, and a double-layer stirring blade 33. The upper end of the liquid injection hollow rod 31 is clamped with the bottom clamping joint 25, and the double-layer stirring blade 33 is fixedly connected to the lower end of the liquid injection hollow rod 31 by a thread.

[0053] The top cover 21 has a first circular through hole in the middle thereof. The first through hole has a horizontally opened circular groove. The support ring 23 is movably connected to the circular groove and can rotate in the circular groove.

[0054] A circular second through hole is provided in the middle of the inner sealing groove 26 , and the inner sealing groove 26 is provided with a thread for connecting with the first layer of thread of the top cover 21 ;

[0055] The pre-setting mechanism 4 is used to pre-store the solid or liquid used in the reaction;

[0056] The sealed liquid injection mechanism 5 is used to isolate the reagents in the micromagnetic control tube 1 from the reagents in the reaction mechanism 6;

[0057] The reaction mechanism 6 is used to clean the nucleic acid to be detected;

[0058] The reaction chamber 7 is used for the reaction between nucleic acid and reaction solution;

[0059] After installation is completed, the double-layer stirring blades 33 are located above the sealed liquid injection mechanism 5 .

[0060] The disclosed embodiment of the present invention is a universal test tube and a detection method based on micromagnetic control technology. The reaction is carried out inside the micromagnetic control tube, and the spiral sealing effect is better at the top. The sealing transmission mechanism has a sealing function and a stirring function, which can reduce the risk of contamination of the sample nucleic acid and allow the reaction to proceed fully through stirring. Paraffin is injected into the inner wall of the inner sealing groove to perform a secondary seal. Moreover, the hollow injection rod of the liquid injection stirring mechanism can facilitate the addition of the sample liquid to the interior of the micromagnetic control tube, and the double-layer stirring blades can stir the reaction liquid more evenly.

[0061] The preset mechanism can have any number of preset slots, and its function is to place various reagents and substances in advance, so that different liquids can be isolated before use. The function of the sealed liquid injection mechanism is to facilitate the injection of various reagents and substances into the reaction tank and to seal it; the various chambers inside the reaction tank can be injected with different liquids, and the specific components in the samples and reagents can be adsorbed by the magnetic beads and can be moved between different liquids under the action of the magnetic beads to achieve the desired effect. Finally, the required substances can be moved together with the magnetic beads to the inside of the reaction chamber for mixing; under specific conditions inside the reaction chamber, a series of specific reactions occur in the internal substances. The inner bottom of the preset slot adopts a sloped opening design to facilitate the flow of reagents out of the preset slot to the inside of the micromagnetic control tube.

[0062] In a possible implementation, the presetting mechanism 4 includes a presetting cover 41 and a presetting groove 42;

[0063] The presetting mechanism 4 includes a presetting cover 41 and a presetting groove 42. The presetting cover 41 has a circular fourth through hole in the middle. The presetting groove 42 has a cylindrical groove in the middle. The shaft seal transmission shaft 22 passes through the cylindrical groove and the fourth through hole.

[0064] The pre-groove 42 is divided into multiple grooves by partitions in the vertical direction, and multiple slots are provided at the bottom of the pre-groove cover 41 to adapt to the positions of the multiple partitions in the pre-groove 42;

[0065] The bottom of the pre-slot 42 is designed with a sloped opening. The pre-slot 42 in the pre-slot mechanism 4 can be any number of pre-slots. Their function is to place various reagents and solid substances in advance and to isolate different liquids and solid substances before use. The inner bottom of the pre-slot 42 adopts a sloped opening design to facilitate the flow of reagents out of the pre-slot 42 into the interior of the micromagnetic control tube 1.

[0066] The universal detection test tube and detection method based on micromagnetic control technology can also be used in other technical fields such as chemiluminescence and chemiluminescent immunoassay technology, using magnetic bead adsorption to achieve the same luminescent detection effect. The other slots of the pre-set slot 42 are reserved for adding other reagents that need to be isolated when using chemiluminescent immunoassay and other methods.

[0067] In a possible implementation, the sealing and liquid injection mechanism 5 includes a sealing plate 51 and a liquid injection plate 52 , and the reaction mechanism 6 includes a first connecting cavity 61 , a first isolation cavity 62 , a cleaning cavity 63 , a second isolation cavity 64 , and a second connecting cavity 65 ;

[0068] The liquid injection plate 52 is provided with a plurality of circular holes connected to the cavities of the reaction mechanism 6, and the lower side of the sealing plate 51 is provided with a plurality of cylindrical protrusions corresponding to the circular holes, which are used to seal the circular holes;

[0069] The second connecting cavity 65 is connected to the reaction cavity 7;

[0070] The reaction chamber 7 is in the shape of a cone that is wider at the top and narrower at the bottom.

[0071] The function of the sealing and liquid injection mechanism 5 is to facilitate the injection of various reagents and substances into the reaction tank and to seal it.

[0072] In a possible implementation, the top clamping joint 24 is connected to an external motor drive mechanism and rotates under the drive of the motor drive mechanism.

[0073] Alternatively, the sealing transmission shaft 22 can be rotated manually to allow the reaction in the test tube to proceed fully, for example, to accelerate the lysis reaction so that the sample is fully lysed.

[0074] The present invention discloses a method for detecting nucleic acid amplification by stirring extraction, which is applied to a universal detection test tube based on micromagnetic control technology. The method comprises:

[0075] Add the reaction solution, paraffin wax, and biological reagent into the reaction chamber 7 in sequence;

[0076] Silicone oil is added to the first isolation chamber 62 and the second isolation chamber 64 of the reaction mechanism 6, and cleaning liquid is added to the cleaning chamber 63; both the silicone oil and the cleaning liquid are used to clean the magnetic beads.

[0077] Solidified paraffin is pre-placed on the bottom of each groove of the pre-grooves 42;

[0078] A magnetic bead mixed liquid is added onto the paraffin at the bottom of one of the pre-set tanks 42 .

[0079] The above steps are for packaging the detection reagent of the micromagnetic test tube.

[0080] Open the lid of the micromagnetic control tube 1, and insert the pipette tip through the hollow injection rod 31 and the double-layer stirring blade 33 to the space between the presetting mechanism 4 and the sealed injection mechanism 5 to add the sample liquid; the micromagnetic control tube 1 is sealed by the top cover 21, the sealing transmission shaft 22 and the inner sealing groove 26;

[0081] Rotate the sealing drive shaft 22 manually or by motor drive;

[0082] The magnetic bead mixed liquid is mixed with the test sample in the pre-groove 42, and the paraffin at the bottom of the pre-groove 42 is melted by heating, and the liquid at the bottom of the pre-groove 42 flows into the middle of the micromagnetic control tube 1;

[0083] Insert the micro-magnetic control tube 1 into the shaftless ball gear, and the test sample begins to heat up and lyse. The external magnet rotates repeatedly to drive the magnetic beads inside the micro-magnetic control tube 1 to move. The lysed nucleic acids combine with the magnetic beads. Under the action of the external magnet, the magnetic beads pass through the first connecting chamber 61, the first isolation chamber 62, the cleaning chamber 63, the second isolation chamber 64, and the second connecting chamber 65 in sequence to complete the cleaning.

[0084] For example, the cleaning chamber 63 contains a cleaning liquid for cleaning impurities on the magnetic beads.

[0085] The paraffin on the upper portion of the reaction chamber 7 is melted by temperature control, and the magnetic beads carrying nucleic acids enter the reaction chamber 7;

[0086] Under the action of the reagents inside the reaction chamber 7 , the magnetic beads are separated from the nucleic acids and fall to the bottom of the reaction chamber 7 . The nucleic acids react with the reaction solution in the reaction chamber 7 , and the detection instrument collects fluorescence from the side of the reaction chamber 7 .

[0087] Application examples using micromagnetron cuvettes.

[0088] The inner bottom of each pre-set groove 42 is pre-set with solidified paraffin wax in advance. Magnetic beads mixed liquid is added to the paraffin inside one of the pre-set grooves 42. Other liquids that need to be isolated and pre-set can be selectively added to other grooves, and the pre-set cover 41 is covered to achieve the purpose of pre-setting.

[0089] When in use, first rotate to open the sealing transmission mechanism 2, use a pipette to insert the TIP head to absorb the sample, and the TIP head passes through the hollow injection rod 31 and the double-layer stirring blade 33 to penetrate between the preset mechanism 4 and the sealed injection mechanism 5 to add the sample liquid into the interior of the micromagnetic control tube. After that, rotate the sealing transmission mechanism 2 to lock the bottom clamping joint 25 with the top clamping groove 32, and then place the test tube on the test equipment to perform the detection reaction.

[0090] During the reaction, the paraffin at the bottom of the pre-groove 42 is melted by heating. Since the inner bottom of the pre-groove 42 adopts a sloped opening design, it is convenient for the paraffin and magnetic bead mixed liquid and other pre-set liquids to flow out of the pre-groove 42 to the middle of the micro-magnetic control tube 1 to mix with the liquid in the middle of the micro-magnetic control tube 1. The rotation of the bottom clamping joint 25 makes the various liquids and magnetic beads mix more fully with the liquid in the middle of the micro-magnetic control tube 1. At the same time, under the action of the external magnet, the magnetic beads will gather just above the first connecting chamber 61. The magnet will move in sequence along the first connecting chamber 61, the first isolation chamber 62, the cleaning chamber 63, and the second isolation chamber 64 until it moves to just above the second connecting chamber 65, and finally fall into the interior of the reaction chamber 7 through the second connecting chamber 65 to react.

[0091] The disclosed embodiments of a universal test tube and detection method based on micromagnetic control technology conduct reactions within the micromagnetic control tube, with a spiral seal at the top for enhanced effectiveness. The sealing transmission mechanism provides both sealing and stirring functions, reducing the risk of sample nucleic acid contamination and ensuring sufficient reaction progress through stirring. Paraffin wax is injected into the inner wall of the inner sealing groove for secondary sealing.

[0092] The preset mechanism can have any number of preset slots, and its function is to place various reagents and substances in advance and isolate them before use. The function of the sealed liquid injection mechanism is to facilitate the injection of various reagents and substances into the reaction tank and seal it; the various slots inside the reaction tank can be injected with different liquids, which can be moved between different liquids under the action of magnetic beads to achieve the desired effect. Finally, the required substances can move together with the magnetic beads to the inside of the reaction chamber for mixing; under specific conditions inside the reaction chamber, the internal substances undergo a series of specific reactions. The inner bottom of the preset slot adopts a sloped opening design to facilitate the flow of reagents out of the preset slot to the inside of the micromagnetic control tube.

[0093] The universal detection test tube based on micromagnetic control technology disclosed in this application can realize universal detection of various samples (such as sputum, blood, cerebrospinal fluid, pleural effusion, body fluids, pathological sections, etc.) based on a micromagnetic control method. The corresponding nucleic acid amplification detection technology and chemiluminescence immunoassay technology detection, etc., are only a specific implementation method of the detection method that can be used by the detection test tube.

[0094] It should be noted that for the aforementioned method embodiments, for simplicity of description, they are all expressed as a series of action combinations, but those skilled in the art should be aware that the present disclosure is not limited by the order of the actions described, because according to the present disclosure, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all optional embodiments, and the actions and modules involved are not necessarily required by the present disclosure.

[0095] In the above embodiments, the description of each embodiment has its own focus. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0096] In the several embodiments provided in the present disclosure, it should be understood that the disclosed devices can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the division of the units is merely a logical function division. In actual implementation, there may be other division methods, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. In addition, the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical or other forms.

[0097] The embodiments of the present disclosure are introduced in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present disclosure. The description of the above embodiments is only used to help understand the method and core ideas of the present disclosure. At the same time, for those skilled in the art, according to the ideas of the present disclosure, there may be changes in the specific implementation methods and application scopes. In summary, the contents of this specification should not be understood as limiting the present disclosure.

[0098] Various aspects of the present disclosure are described herein with reference to flowcharts and / or block diagrams of methods, apparatus (systems), and computer program products according to embodiments of the present disclosure. It should be understood that each block of the flowcharts and / or block diagrams, and combinations of blocks in the flowcharts and / or block diagrams, can be implemented by computer-readable program instructions.

[0099] While various embodiments of the present disclosure have been described above, the foregoing description is intended to be illustrative, non-exhaustive, and not limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is selected to best explain the principles of the embodiments, their practical applications, or technological improvements in the marketplace, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A universal detection test tube based on micromagnetic control technology, characterized in that: It comprises a micromagnetic control tube (1) and a sealing transmission mechanism (2), wherein the top of the micromagnetic control tube (1) is provided with the sealing transmission mechanism (2), and the bottom of the micromagnetic control tube (1) is provided with a reaction chamber (7); The sealing transmission mechanism (2) comprises a top cover (21), the top cover (21) being arranged on the top of the micromagnetic control tube (1), the top cover (21) being provided with a first layer of threads for connecting with an inner sealing groove (26) and a second layer of threads for connecting with a pipe mouth of the micromagnetic control tube (1), a sealing transmission shaft (22) being arranged inside the top cover (21), a top clamping joint (24) being arranged at the upper end of the sealing transmission shaft (22), a supporting ring (23) having an inner diameter greater than that of the sealing transmission shaft (22) being arranged below the sealing transmission shaft (22), a bottom clamping joint (25) having an inner diameter less than that of the supporting ring (23) being arranged below the supporting ring (23), the sealing transmission shaft (22), the supporting ring (23), the top clamping joint (24) and the bottom clamping joint (25) being an integral body; A liquid injection stirring mechanism (3) is arranged below the sealing transmission mechanism (2), and the liquid injection stirring mechanism (3) is used for performing liquid injection stirring. A pre-setting mechanism (4) is arranged outside the liquid injection stirring mechanism (3), and the pre-setting mechanism (4) is used for pre-storing solids or liquids used for the reaction. A sealed liquid injection mechanism (5) and a reaction mechanism (6) are provided below the liquid injection and stirring mechanism (3). The sealed liquid injection mechanism (5) is used to isolate the reagents in the micromagnetic control tube (1) from the reagents in the reaction mechanism (6); the reaction mechanism (6) is used to clean the nucleic acid to be detected; and the reaction chamber (7) is used to react the nucleic acid with the reaction liquid.

2. A universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: A first circular through hole is provided in the middle of the top cover (21), the first through hole has a horizontally opened circular groove, the support ring (23) is movably connected to the circular groove, and the support ring (23) rotates in the circular groove; a second circular through hole is provided in the middle of the inner sealing groove (26), and the inner sealing groove (26) is provided with a thread for connecting with the first layer of threads of the top cover (21).

3. A universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: The top clamping joint (24) is connected to an external motor drive mechanism and rotates under the drive of the motor drive mechanism.

4. The universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: The liquid injection stirring mechanism (3) comprises a liquid injection hollow rod (31), a top clamping groove (32) and a double-layer stirring blade (33), the upper end of the liquid injection hollow rod (31) is clamped with the bottom clamping joint (25), the double-layer stirring blade (33) is spirally fixedly connected with the lower end of the liquid injection hollow rod (31), and the double-layer stirring blade (33) is located above the sealed liquid injection mechanism (5).

5. The universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: The presetting mechanism (4) comprises a presetting cover (41) and a presetting groove (42); a fourth circular through hole is provided in the middle of the presetting cover (41); a cylindrical groove is provided in the middle of the presetting groove (42); and the sealing transmission shaft (22) passes through the cylindrical groove and the fourth through hole.

6. A universal detection test tube based on micromagnetic control technology according to claim 5, characterized in that: The pre-set groove (42) is divided into a plurality of grooves by partitions in the vertical direction, a plurality of slots are arranged at the bottom of the pre-set cover (41) to adapt to the positions of the plurality of partitions in the pre-set groove (42), and the bottom of the pre-set groove (42) is designed as a sloped opening.

7. The universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: The sealed liquid injection mechanism (5) comprises a sealing plate (51) and a liquid injection plate (52), wherein the liquid injection plate (52) is provided with a plurality of circular holes connected to the respective cavities of the reaction mechanism (6), and the lower side of the sealing plate (51) is provided with a plurality of cylindrical protrusions corresponding to the circular holes, wherein the protrusions are used to seal the circular holes.

8. The universal detection test tube based on micromagnetic control technology according to claim 1, characterized in that: The reaction mechanism (6) comprises a first connecting chamber (61), a first isolation chamber (62), a cleaning chamber (63), a second isolation chamber (64) and a second connecting chamber (65) which are arranged in a circumferential direction. The second connecting chamber (65) is connected to the reaction chamber (7). The reaction chamber (7) is in the shape of a cone which is wide at the top and narrow at the bottom.

9. A method for detecting nucleic acid amplification by stirring extraction, characterized in that: The method is applied to a universal detection test tube based on micromagnetic control technology as described in any one of claims 1 to 8, and the method comprises: Open the lid of the micromagnetic control tube (1) and add the sample into the space between the preset mechanism (4) and the sealed liquid injection mechanism (5) in the micromagnetic control tube (1); The micromagnetic control tube (1) is sealed by means of a top cover (21), a sealing transmission shaft (22) and an inner sealing groove (26); Rotating the sealing transmission shaft (22) manually or by motor drive; The magnetic bead mixed liquid is mixed with the test sample in the pre-groove (42), the paraffin at the bottom of the pre-groove (42) is melted by heating, and the liquid at the bottom of the pre-groove (42) flows into the middle of the micromagnetic control tube (1); The micromagnetic control tube is inserted into the axisless ball gear, the test sample starts to heat up and lyse, the external magnet rotates repeatedly to drive the magnetic beads inside the micromagnetic control tube (1) to move, the lysed nucleic acid is combined with the magnetic beads, and under the action of the external magnet, the magnetic beads pass through the first connecting cavity (61), the first isolation cavity (62), the cleaning cavity (63), the second isolation cavity (64), and the second connecting cavity (65) in sequence to complete the cleaning; The paraffin on the upper part of the reaction chamber (7) is melted by temperature control, and the magnetic beads carrying the nucleic acid enter the reaction chamber (7); Under the action of the reagent inside the reaction chamber (7), the magnetic beads are separated from the nucleic acid, the magnetic beads are moved to the bottom of the reaction chamber (7), the nucleic acid reacts with the reaction liquid in the reaction chamber (7), and the detection instrument collects fluorescence from the side of the reaction chamber (7).

10. A method for detecting nucleic acid amplification by stirring extraction according to claim 9, characterized in that: The method further comprises the following steps before performing the detection: Adding reaction solution, paraffin wax and biological reagent into the reaction chamber (7) in sequence; Adding silicone oil into the first isolation chamber (62) and the second isolation chamber (64) of the reaction mechanism (6), and adding cleaning liquid into the cleaning chamber (63); Solidified paraffin is pre-placed on the bottom of each groove of the pre-groove (42); A magnetic bead mixed liquid is added onto the paraffin at the bottom of one of the pre-set grooves (42).

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