Mycelium growth method, mycelium and use

Abstract

The present application relates to the technical field of mycelia, and discloses a mycelium growth method, a mycelium and a use. The method comprises: soaking a porous material in a nutrient solution, taking out the porous material after the porous material is fully soaked, and drying; inoculating a ganoderma lucidum strain on a macropore wall of the dried porous material for culture, so as to obtain a ganoderma lucidum strain cultured by the porous material; and placing the ganoderma lucidum strain cultured by the porous material at the bottom of a container containing the nutrient solution, partially soaking the porous material in the nutrient solution, and culturing the strain in a closed space. The porous material used in the method has a uniform structure and is beneficial to uniform growth of a hypha. The porous material has certain strength and a smooth surface, thereby facilitating the separation of the hypha and a substrate, and the activity of a grown mycelium is high.

Description

Mycelium growth method, mycelium and application Technical Field

[0001] The present invention relates to the technical field of mycelium, and in particular to a mycelium growth method, mycelium and applications. Background Art

[0002] Currently, the main method for growing mycelium is the solid culture method. Due to the unevenness of the biomass particles and the unevenness of the internal pores of the solid culture medium, the mycelial morphology obtained by this method is uneven, and the growing mycelium is not easy to peel off from the substrate. In addition, the speed at which Ganoderma lucidum mycelium digests solid nutrients during growth is relatively slow compared to liquid nutrient solutions. The solid culture method requires stable humidity control, which also increases costs. Solid culture medium is difficult to replace nutrients midway through mycelial cultivation. As the mycelium grows, the nutrients will change, which is not conducive to the subsequent growth of mycelium.

[0003] Currently, some use liquid culture methods, culturing Ganoderma lucidum mycelium in a fermentation broth to produce mycelial gel materials. However, the mycelium cultivated in this way is highly hydrophilic, resulting in brittleness after drying. Aerial mycelium obtained through solid culture methods, on the other hand, has a high hydrophobin content and, after drying, weak hydrogen bonding between mycelium, which improves the flexibility of the mycelium material. Furthermore, mycelium in liquid fermentation broth is shorter and coarser than that obtained through solid fermentation methods, resulting in poor strength and flexibility after drying.

[0004] In view of this, the present invention is proposed. Summary of the Invention

[0005] To solve the problems in the background technology, the present invention provides a mycelium growth method, mycelium and applications.

[0006] The mycelium grows faster under this growth method, and a mycelium material with higher activity can be effectively obtained. No additional humidity control is required during the growth process, and the mycelium can grow continuously and be harvested regularly to obtain the mycelium material.

[0007] In order to achieve the above object, the first technical solution adopted by the present invention is:

[0008] Mycelium growth methods, including:

[0009] Soaking the porous material in the nutrient solution, and after it is fully soaked, taking out the porous material and drying it;

[0010] inoculating the pretreated Ganoderma lucidum strains onto the macropore walls of the dried porous material for cultivation to obtain Ganoderma lucidum strains cultured on the porous material;

[0011] The ganoderma lucidum strains cultured on the porous material are placed at the bottom of a container containing a nutrient solution, so that the porous material is partially immersed in the nutrient solution and cultured in a closed space.

[0012] Preferably, the porous material is a hydrophilic material having a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the regular vertical pores is 2 mm-5 mm, and the pore diameter of the small pores is 10 um-300 um.

[0013] Preferably, the pretreated Ganoderma lucidum strains refer to Ganoderma lucidum strain balls prepared by liquid fermentation method.

[0014] Preferably, the nutrient solution comprises 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 vitamin B and 0.1-0.5 g MgSO4, and 1 L deionized water.

[0015] Preferably, the Ganoderma lucidum strains cultured on the porous material are placed at the bottom of a container containing a nutrient solution, so that 10%-50% of the height of the porous material is in the nutrient solution.

[0016] Preferably, the container containing the nutrient solution has an open top.

[0017] Preferably, when culturing in a closed space, the temperature of the closed space is 23-32° C., the carbon dioxide concentration is 3%-15%, and the culturing time is 7-21 days.

[0018] The second technical solution of the present application is: mycelium is obtained by any of the above-mentioned growth methods.

[0019] The third technical solution of the present application is: the application of the mycelium described above as mycelium bio-based material.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention utilizes a specific porous material to inoculate Ganoderma lucidum strains onto the macropore walls of the porous material. The porous material can utilize capillary action to continuously deliver nutrients from the nutrient solution in the container to the mycelium through the small pores on the pore walls, while maintaining the nutrient solution components and oxygen content required for the growth process, fully meeting the nutritional elements required for mycelium growth and ensuring the activity of the mycelium. The embodiments of the present invention do not require additional humidity control. The liquid culture medium and the nutritional stability of the culture medium can accelerate mycelium growth and increase mycelium activity. The uniform structure of the porous material is conducive to the uniform growth of mycelium. The porous material has a certain strength and a smooth surface, which is conducive to the separation of mycelium from the substrate. In addition, the present invention can achieve continuous growth and regular harvesting of mycelium material without the need to re-prepare the culture medium. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with specific embodiments.It should be understood that these descriptions are exemplary only, and are not intended to limit the scope of the present invention.In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.Unindicated specific conditions in the embodiment are carried out according to the conditions of normal conditions or manufacturer's advice.Reagents used or instruments are not indicated by manufacturers, and are conventional products that can be obtained by commercial purchase.

[0023] An embodiment of the present invention provides a method for growing mycelium, comprising: soaking a porous material in a nutrient solution, and after sufficient infiltration, taking out the porous material and drying it; inoculating Ganoderma lucidum strains onto the macropore walls of the dried porous material for cultivation to obtain Ganoderma lucidum strains cultured on the porous material; placing the Ganoderma lucidum strains cultured on the porous material at the bottom of a container containing a nutrient solution, so that the porous material is partially immersed in the nutrient solution, and cultivating in a closed space.

[0024] It should be noted that the nutrient solution is a liquid culture medium, and the porous material inoculated with the Ganoderma lucidum strain is partially immersed in the liquid culture medium during the culture process.

[0025] The embodiments of the present invention utilize a specific porous material to inoculate Ganoderma lucidum spawn onto the macropore walls of the porous material. The porous material utilizes capillary action to continuously deliver nutrients from the nutrient solution in the container to the mycelium through the pores in the pore walls, while maintaining the nutrient solution components and oxygen content required for growth, fully meeting the nutritional requirements of the mycelium during growth and ensuring mycelial activity. The embodiments of the present invention eliminate the need for additional humidity control, maintain the nutritional stability of the liquid culture medium and the culture medium, and can accelerate mycelial growth and enhance mycelial activity. The porous material has a uniform structure that facilitates uniform mycelial growth. The porous material also has a certain strength and a smooth surface, which facilitates the separation of mycelium from the substrate.

[0026] In this embodiment, the Ganoderma lucidum spawn pellet adheres to the walls of the macropores of the porous material. A portion of the pellet contacts the nutrient solution through the porous material's smaller pores, while the remaining portion remains exposed to the liquid surface. As the aerial hyphae grow, they gradually fill the macropores. Respiration creates an oxygen concentration gradient from bottom to top, forcing the hyphae to absorb oxygen more efficiently. Eventually, a mycelial foam of a certain height forms above the porous material, ready for harvesting.

[0027] The porous material is a hydrophilic material with a regular vertical pore structure. The pore walls are composed of countless continuous small pores. These regular vertical pores are large pores, which are used to support the mycelium balls. After the large pores absorb water, they will not clog the large pores, ensuring that the mycelium balls have an adequate oxygen supply. The small pores in the pore walls are used to transport nutrients to the mycelium loaded on the large pores through capillary action. Therefore, the pore diameters of the large and small pores only need to meet the above conditions. In a preferred embodiment, the pore diameter of the large pores is 2 mm to 5 mm, and the pore diameter of the small pores is 10 μm to 300 μm.

[0028] Regarding the thickness of the porous material, during the culture process, the porous material needs to be partially immersed in the nutrient solution to better transport nutrients. Therefore, if the porous material is too thin, the portion not immersed in the nutrient solution can only support a small amount of mycelium, and a good oxygen concentration gradient cannot be formed during the growth process. Those skilled in the art can adjust the thickness of the porous material according to actual conditions. In some preferred embodiments, the thickness of the porous material is 2 cm to 10 cm.

[0029] The nutrient components are commonly used in liquid culture media and are not specifically limited. Those skilled in the art can adjust the composition ratio as needed. In a preferred embodiment, the nutrient solution comprises 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 vitamin B6, and 0.1-0.5 g MgSO4, in 1 L of deionized water.

[0030] The container is open at the top. The Ganoderma lucidum cultured on the porous material is placed at the bottom of the container containing a nutrient solution, with the porous material partially immersed in the nutrient solution. If the nutrient solution level is too low, the capillary action of the porous material will be affected. If the nutrient solution level is too high, more of the porous material will be submerged, resulting in oxygen deprivation within the macropores of the porous material, which is not conducive to the growth of aerial hyphae. In a preferred embodiment, 10% to 50% of the height of the porous material is immersed in the nutrient solution.

[0031] The parameters for cultivation in a confined space are not specifically defined and are commonly used in mycelium cultivation. Those skilled in the art can adjust these parameters based on actual needs. In a preferred embodiment, the temperature in the confined space is controlled at 23-32°C, the carbon dioxide concentration is 3%-15%, and the culture is carried out for 7-21 days. After completion of the culture, the Ganoderma mycelium grown on the porous material can be harvested with a knife.

[0032] In an embodiment of the present invention, liquid culture medium is used as a nutrient source to culture mycelium, and a solid porous material is used as a growth carrier of the mycelium. During the growth process, the mycelium is loaded on the porous material, and the mycelium absorbs the nutrients in the liquid nutrient solution and continuously grows upward to a certain thickness, making it easy to harvest clean mycelium material.

[0033] The harvested Ganoderma mycelium is dried to obtain Ganoderma mycelium bio-based material, which has significantly improved strength and flexibility.

[0034] To make the technical solution of the present invention clearer, the following describes the mycelium growth method, application and performance through multiple embodiments. Example 1

[0035] The porous material has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 2mm, the pore diameter of the small pore is 50um, and the thickness is 5cm.

[0036] The porous material was immersed in a solution consisting of 15 g D-glucose, 2.5 g peptone, 3 g yeast extract, 0.1 g KH2PO4, 0.2 g K2HPO4, 0.1 g vitamin B and 0.1 MgSO4 in 1 L deionized water. After being fully soaked, it was taken out and dried.

[0037] The liquid fermented Ganoderma lucidum strains are inoculated onto the macropore walls of the dried porous material for cultivation to obtain the Ganoderma lucidum strains cultured on the porous material.

[0038] The Ganoderma lucidum cultured on the porous material was placed at the bottom of a container containing a nutrient solution consisting of 15 g D-glucose, 2.5 g peptone, 3 g yeast extract, 0.1 g KH2PO4, 0.2 g K2HPO4, 0.1 g vitamin B and 0.1 g MgSO4, in 1 L of deionized water. The height of the solution was controlled to be 5 mm.

[0039] The above container was placed in a closed space, the temperature in the closed space was controlled to be 23°C, the carbon dioxide concentration was 3%, and the culture was carried out for 14 days. Then, the Ganoderma mycelium growing on the porous material was harvested with a knife.

[0040] The harvested Ganoderma mycelium was dried at 40°C and then pressed at a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma mycelium bio-based material. Example 2

[0041] A porous material is provided, which has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 um, and the thickness is 2 cm.

[0042] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 MgSO4 in 1 L deionized water. After being fully soaked, it was taken out and dried.

[0043] The liquid fermented Ganoderma lucidum strains are inoculated onto the macropore walls of the dried porous material for cultivation to obtain the Ganoderma lucidum strains cultured on the porous material.

[0044] The Ganoderma lucidum cultured on the porous material was placed at the bottom of a container containing a nutrient solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 g MgSO4, in 1 L of deionized water. The height of the solution was controlled to be 2 mm.

[0045] The above container is placed in a closed space, the temperature in the closed space is controlled to 26°C, the carbon dioxide concentration is 5%, and culture is carried out for 14 days. Then, the Ganoderma mycelium growing on the porous material is harvested with a knife.

[0046] The harvested Ganoderma mycelium was dried at 40° C. and then pressed at a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma mycelium bio-based material. Example 3

[0047] A porous material is provided, which has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 um, and the thickness is 2 cm.

[0048] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 MgSO4 in 1 L deionized water. After being fully soaked, it was taken out and dried.

[0049] The liquid fermented Ganoderma lucidum strains are inoculated onto the macropore walls of the dried porous material for cultivation to obtain the Ganoderma lucidum strains cultured on the porous material.

[0050] The Ganoderma lucidum cultured on the porous material was placed at the bottom of a container containing a nutrient solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 g MgSO4, in 1 L of deionized water. The height of the solution was controlled to be 2 mm.

[0051] The above container is placed in a closed space, the temperature in the closed space is controlled to 26 ° C, the carbon dioxide concentration is 5%, and cultured for 14 days. Then, the Ganoderma mycelium growing on the porous material is harvested with a knife to complete the first harvest.

[0052] The Ganoderma mycelium obtained by the first harvest is dried to obtain the Ganoderma mycelium bio-based material.

[0053] Drain the mixed solution added to the container, re-prepare the same nutrient solution, and introduce it into the container, controlling the solution height to 2 mm.

[0054] The container with the replaced nutrient solution was placed in a closed space, and the temperature in the closed space was controlled to be 26°C and the carbon dioxide concentration was 5%. The culture was carried out for 14 days, and then the Ganoderma lucidum mycelium growing on the porous material was harvested with a knife to complete the second harvest.

[0055] The Ganoderma mycelium obtained from the second harvest was dried at 40°C and then pressed at a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma mycelium bio-based material. It can be seen that the present invention can achieve regular harvesting of the mycelium by regularly replacing the nutrient solution. Example 4

[0056] A porous material is provided, which has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 3 mm, the pore diameter of the small pore is 150 um, and the thickness is 5 cm.

[0057] The porous material was immersed in a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 MgSO4 in 1 L deionized water. After being fully soaked, it was taken out and dried.

[0058] The liquid fermented Ganoderma lucidum strains are inoculated onto the macropore walls of the dried porous material for cultivation to obtain the Ganoderma lucidum strains cultured on the porous material.

[0059] The Ganoderma lucidum cultured on the porous material was placed at the bottom of a container containing a nutrient solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B and 0.2 g MgSO4, in 1 L of deionized water. The height of the solution was controlled to be 10 mm.

[0060] The above container is placed in a closed space, the temperature in the closed space is controlled to 26°C, the carbon dioxide concentration is 5%, and culture is carried out for 14 days. Then, the Ganoderma mycelium growing on the porous material is harvested with a knife.

[0061] The harvested Ganoderma mycelium was dried at 40° C. and then pressed at a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma mycelium bio-based material.

[0062] Comparative Example 1

[0063] Compared to Example 1, the only difference is that the large pore diameter of the porous material is adjusted to 0.3 mm. The remaining components and steps are the same as in Example 1. Specifically, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of countless continuous small pores. The large pore diameter is 0.3 mm, the small pore diameter is 50 μm, and the thickness is 5 cm.

[0064] Comparative Example 2

[0065] Compared to Example 1, the only difference is that the large pore diameter of the porous material is adjusted to 10 mm. The remaining components and steps are the same as in Example 1. Specifically, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of countless continuous small pores. The large pore diameter is 10 mm, the small pore diameter is 50 μm, and the thickness is 5 cm.

[0066] Comparative Example 3

[0067] Compared to Example 1, the only difference is that the pore diameter of the porous material is adjusted to 1 μm. The remaining components and steps are the same as in Example 1. Specifically, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of countless continuous pores. The large pores have a diameter of 2 mm, the small pores have a diameter of 1 μm, and the thickness is 5 cm.

[0068] Comparative Example 4

[0069] Compared to Example 1, the only difference is that the pore diameter of the porous material is adjusted to 500 μm. The remaining components and steps are the same as in Example 1. Specifically, the porous material in this comparative example has a regular vertical pore structure, with the pore walls composed of countless continuous pores. The large pores have a diameter of 2 mm, the small pores have a diameter of 500 μm, and the thickness is 5 cm.

[0070] Comparative Example 5

[0071] Compared with Example 2, the only difference is that the thickness of the porous material is adjusted to 0.2 cm, and the remaining components and steps are the same as Example 2. That is, the porous material of this comparative example has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 μm, and the thickness is 0.2 cm.

[0072] Comparative Example 6

[0073] Compared with Example 2, the only difference is that the height of the solution is controlled to 14 mm, and the other components and steps are the same as Example 2. That is, the porous material of this comparative example has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 μm, and the thickness is 2 cm.

[0074] Comparative Example 7

[0075] Compared with Example 2, the only difference is that the height of the solution is controlled to 0.5 mm, and the other components and steps are the same as Example 2. That is, the porous material of this comparative example has a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the large pore is 5 mm, the pore diameter of the small pore is 300 μm, and the thickness is 2 cm.

[0076] Comparative Example 8

[0077] Compared with Example 2, the only difference is that the pore diameter of the porous material is 5 mm, and the other components and steps are the same as Example 2. That is, the porous material of this comparative example has a regular vertical pore structure, the pore wall is composed of countless continuous and uniform pores, the pore diameter is 5 mm, and the thickness is 2 cm.

[0078] Comparative Example 9

[0079] Compared with Example 2, the only difference is that the pore diameter of the porous material is 300 μm, and the remaining components and steps are the same as Example 2. That is, the porous material of this comparative example has a regular vertical pore structure, the pore wall is composed of countless continuous and uniform pores, the pore diameter is 300 μm, and the thickness is 2 cm.

[0080] Comparative Example 10

[0081] Compared with Example 2, the only difference is that the use of porous materials is eliminated, and the Ganoderma lucidum strains are directly inoculated into the liquid culture medium for cultivation, as follows:

[0082] S1: Prepare liquid culture medium: a solution consisting of 10 g D-glucose, 2 g peptone, 1.5 g yeast extract, 0.2 g KH2PO4, 0.4 g K2HPO4, 0.3 g vitamin B6, and 0.2 g MgSO4 in 1 L deionized water.

[0083] S2: inoculate the liquid fermented Ganoderma lucidum strain into the culture medium in step 1 for cultivation.

[0084] S3: Place the cultured Ganoderma lucidum strains from step 2 in an open mold. Place the mold in a sealed space and maintain the temperature at 26°C and the CO2 concentration at 5%. Cultivate for 14 days.

[0085] S4: Dry the Ganoderma lucidum mycelium material obtained in step 3 at 40° C., and then press the material under a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma lucidum mycelium bio-based material.

[0086] Comparative Example 11

[0087] Compared with Example 2, the difference is that the use of porous materials is eliminated and the liquid culture medium is replaced by the existing solid culture medium, as follows:

[0088] S1: Prepare solid culture medium: 78 g poplar wood powder, 20 g wheat bran, 1 g calcium sulfate, 1 g sucrose, water content of 65%,

[0089] S2: Sterilize 100 g of medium 1 at 121°C for 30 minutes, then cool to room temperature for later use.

[0090] S3: Inoculate 10 ml of liquid white rot fungus mycelium into the mixture of step 2, incubate at 24°C for 3 days, then crush the mixture, place it in a 21 cm * 15 cm * 4 cm plastic dish, flatten it, and incubate it for 4 days.

[0091] S4: Place the three biomass pellet and mycelium mixtures in an open mold. Place the mold in a sealed space at a controlled temperature of 26°C and a carbon dioxide concentration of 5%. Incubate for 14 days.

[0092] S5: Dry the Ganoderma lucidum mycelium material obtained in 4 at 40° C., and then press it at a pressure of 0.2 MPa for 5 hours to obtain the Ganoderma lucidum mycelium bio-based material.

[0093] Experimental example

[0094] The performance of the Ganoderma lucidum mycelium bio-based materials obtained in Examples 1-4 and Comparative Examples 1-11 was tested.

[0095] Mechanical Properties: Tensile strength and elongation at break were measured using an Instron 5969 electronic universal testing machine with a 500 N load cell. The test environment was 23°C and 50% humidity. Each sample was tested five times and the average value was obtained.

[0096] Contact angle test: The water contact angle was measured by a contact angle meter (Krüss, DSA30, Germany).

[0097] The test results are shown in Table 1.

[0098] Table 1

[0099] Contact angle (°) Tensile strength (MPa) 5-day growth height (cm) Elongation at break (%) Folded 180 degrees Example 1 12 18.7 5.1 19.2 Good Example 2 12 37.9 4.6 18.7 Good Example 3 First harvest 12 28.15.4 20.3 Good Example 3 Second harvest 12 17.9 5.1 19.8 Good Example 4 12 28.2 4.9 19.7 Good Comparative example 1 12 37.5 0.2 19.5 Good Comparative Example 2--Unable to form a film--Comparative Example 3--Unable to form a film--Comparative Example 4--Unable to form a film--Comparative Example 5 1227.60.318.2Good Comparative Example 6 1206.91.817.6Good Comparative Example 7 1237.72.618.3Good Comparative Example 8--Unable to form a film--Comparative Example 9 1227.50.217.8Good Comparative Example 10 1023-2.4Broken Comparative Example 1 11248.32.821.1Good

[0100] Note: “-” in the table means that mycelium material cannot be obtained under these conditions and the data test cannot be performed.

[0101] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A method for growing mycelium, characterized in that: include: Soaking the porous material in the nutrient solution, after it is fully soaked, taking out the porous material and drying it; inoculating the pretreated Ganoderma lucidum strains onto the macroporous walls of the dried porous material for culturing, thereby obtaining Ganoderma lucidum strains cultured on the porous material; The ganoderma lucidum strains cultured on the porous material are placed at the bottom of a container containing a nutrient solution, so that the porous material is partially immersed in the nutrient solution and cultured in a closed space.

2. The growth method according to claim 1, characterized in that: The porous material is a hydrophilic material with a regular vertical pore structure, the pore wall is composed of countless continuous small pores, the pore diameter of the regular vertical pores is 2 mm-5 mm, and the pore diameter of the small pores is 10 um-300 um.

3. The growth method according to claim 1, characterized in that The pretreated Ganoderma lucidum strains refer to Ganoderma lucidum strain balls prepared by liquid fermentation method.

4. The growth method according to claim 1, characterized in that The nutrient solution comprises 5-15 g D-glucose, 1-2.5 g peptone, 1-3 g yeast extract, 0.2-1 g KH2PO4, 0.2-1 g K2HPO4, 0.1-0.5 vitamin B and 0.1-0.5 g MgSO4, and 1 L deionized water.

5. The method according to claim 1, characterized in that The ganoderma lucidum strains cultured on the porous material are placed at the bottom of a container containing a nutrient solution, so that 10%-50% of the height of the porous material is immersed in the nutrient solution.

6. The method according to claim 1, characterized in that The container containing the nutrient solution has an opening at the top.

7. The method according to claim 1, characterized in that When culturing in a closed space, the temperature of the closed space is 23-32°C, the carbon dioxide concentration is 3%-15%, and the culturing time is 7-21 days.

8. Mycelium obtained by the growth method according to any one of claims 1 to 6.

9. Use of the mycelium as claimed in claim 7 as mycelium bio-based material.