Cosmetic composition for Anti-inflammation, moisturization, or skin regeneration

Abstract

The present invention relates to a cosmetic composition or quasi-drug composition comprising a patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil, and the inclusion of all the ingredients can exhibit excellent anti-inflammatory, moisturizing, and skin-regenerating efficacy.

Description

Anti-inflammatory, moisturizing, or skin regeneration cosmetic composition

[0001] The present invention relates to a cosmetic composition or a pharmaceutical composition for anti-inflammatory, moisturizing, or skin regeneration.

[0002] The skin is a vital organ responsible for various physiological functions, including protecting the body from the external environment, acting as a barrier to prevent the loss of internal moisture and beneficial substances, temperature regulation, and excretion. However, skin is increasingly damaged by internal and external factors, weakening its protective functions. This can lead to inflammation, decreased skin moisture, and impaired cell protection and proliferation.

[0003] Inflammation is the body's immune response to injury or disease. Oxidative stress, such as UV rays, reactive oxygen species, and free radicals, activate inflammatory factors, causing various diseases and skin aging. Lymphokines such as interleukin-6 (IL-6) and interleukin-8 (IL-8) are responsible for this inflammatory response, and are metabolized into prostaglandins, which mediate various inflammatory responses. Various anti-inflammatory agents are being studied to alleviate inflammation by removing the inflammatory agent and reducing biological responses and symptoms.

[0004] Maintaining skin with adequate moisture by providing it with hydration can improve skin elasticity, anti-aging, and wrinkle improvement. Hyaluronic acid production is known to be directly related to skin moisture content, and the effect on skin hydration can be determined by examining the amount of hyaluronic acid produced and the expression levels of the hyaluronic acid synthesizing enzymes HAS2 and HAS3.

[0005] Skin regeneration occurs as our body attempts to protect damaged skin. It involves a complex biological process, including cell differentiation and replication, matrix protein synthesis, and angiogenesis, as the tissue responds to damage. As skin can be wounded or damaged by internal and external factors, skin regeneration, which helps repair these wounds, is becoming increasingly important.

[0006] The present inventors conducted research on the development of a composite material exhibiting anti-inflammatory, moisturizing, and skin regeneration effects by combining individual materials whose efficacy had not been confirmed, and as a result, they discovered that when 10 specific ingredients were combined, the anti-inflammatory, moisturizing, and skin regeneration effects were significantly improved, thereby completing the present invention.

[0007] The present invention aims to provide a cosmetic composition or a pharmaceutical composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0008] The present invention also aims to provide an anti-inflammatory, skin moisturizing, or skin regeneration cosmetic composition or quasi-drug composition comprising the above components.

[0009] The present invention provides a cosmetic composition or a pharmaceutical composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0010] The present invention provides an anti-inflammatory, moisturizing or skin regeneration method comprising a step of applying or administering to the skin of a subject a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0011] The present invention provides the use of a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil for preparing an anti-inflammatory, moisturizing, or skin regeneration composition.

[0012] The composition according to the present invention can exhibit excellent anti-inflammatory, skin moisturizing, or skin regeneration effects by including a complex of 10 kinds of patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil, and can be effectively used in the fields of cosmetics or quasi-drugs for anti-inflammatory, moisturizing, or skin regeneration.

[0013] Figure 1 is an optical microscope image confirming the in vitro wound healing efficacy according to Example 3-2.

[0014] The present invention is described in detail below.

[0015] Each description and embodiment disclosed herein may also be applied to other descriptions and embodiments. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. Furthermore, the scope of the present invention is not limited by the specific descriptions described below.

[0016] When a part is said to "include" a component, this does not mean that it excludes other components, but rather that it may have other components, unless otherwise stated.

[0017] The present invention provides a cosmetic composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0018] In the present invention, "patchouli" is also called Guangguakhyang, belongs to the Lamiaceae family, and is a plant widely cultivated in the Philippines, India, southern China, etc., and may refer to the dried aerial part of the perennial herb Pogostemon cablin Bentham. In addition, "patchouli extract" can be obtained through a step of ripening and drying patchouli leaves and then extracting them by adding an extraction solvent. The extraction can be performed using a conventional solvent according to a conventional method known in the art for extracting extracts from natural products, and under conventional temperature and pressure conditions. The extraction solvent may be water, a C1-C4 lower alcohol, or a mixture thereof, but is not limited thereto.

[0019] In the present invention, “glycerin” can be obtained through a conventional method known in the art, and can be obtained, for example, by hydrolyzing a vegetable oil such as palm oil.

[0020] In the present invention, "Macadamia Oil" can be obtained through a conventional method known in the art, for example, macadamia can be dried, shells removed, roasted, and pressed with an oil extractor to obtain crude oil, which can then be purified.

[0021] In the present invention, "shea butter" can be obtained through a conventional method known in the art, for example, it can be manufactured by mechanically pressing the fruit of the shea tree, i.e., shea nuts, and extracting the raw oil with an organic solvent such as hexane. Thereafter, the raw oil can be subjected to degumming, neutralization, decolorization, hardening, and deodorization processes to remove phospholipids, thereby obtaining shea butter.

[0022] In the present invention, "sunflower seed oil" refers to oil obtained from sunflower seeds. It is widely used as a massage oil because it has the effect of providing flexibility and moisture to the skin. Sunflower oil can be obtained through conventional methods known in the art, and for example, it can be manufactured by removing the hulls of sunflower seeds, crushing them, and then pressing them. In particular, sunflower seed germs are steamed and heated to soften them, and then pressed, extracted with an organic solvent such as hexane to obtain crude oil, which can then be refined and used.

[0023] In the present invention, "Moringa Oil" may refer to oil extracted from the seeds of the Moringa tree (Moringa oleifera). Moringa oil may be obtained through conventional methods known in the art, for example, by drying Moringa seeds, crushing and pressing them, and then extracting them, followed by deodorizing and filtering them after extraction, but is not limited thereto, and may be obtained through conventional methods known in the art.

[0024] In the present invention, "castor oil" can be obtained through conventional methods known in the art. For example, it can be obtained from castor seeds by separating the seeds from the castor bean fruit, drying and crushing them, and then pressing them. As with other oils, the obtained crude oil can be refined and used.

[0025] In the present invention, "argan oil" can be obtained through a conventional method known in the art, for example, by drying argan fruits, removing the skin, collecting the kernels, cold pressing to obtain crude oil, and then removing wax and distillation through an oil precipitation and filtration process.

[0026] In the present invention, "tocopherol" can be obtained by refining a deodorized distillate obtained after deodorizing vegetable oil. The refining or deodorizing method can utilize any conventional refining or deodorizing method known in the art.

[0027] In the present invention, "Jojoba Oil" can be obtained through a conventional method known in the art, for example, by drying jojoba seeds, removing the shell, pressing at low temperature, and then filtering.

[0028] In the present invention, the composition of the present invention may include 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil.

[0029] In the present invention, each component may be included in the following ratios.

[0030] The weight ratio of patchouli extract: glycerin: macadamia oil: shea butter: sunflower oil: moringa oil: castor oil: argan oil: tocopherol: jojoba oil can be 1: 0.5~1: 0.05~0.3: 0.05~0.4: 0.005~0.03: 0.005~0.03: 0.005~0.03: 0.005~0.03: 0.005~0.03: 0.005~0.03, and 1: 0.5~0.8: 0.05~0.2: 0.1~0.3: 0.005~0.02: 0.01~0.03: 0.01~0.03: 0.005~0.02 : It can be 0.01~0.03.

[0031] In the present invention, patchouli extract may be included in an amount of 0.001 to 1 wt% based on the total weight of the composition, glycerin may be included in an amount of 0.0001 to 0.1 wt% based on the total weight of the composition, macadamia oil may be included in an amount of 0.0001 to 0.1 wt% based on the total weight of the composition, shea butter may be included in an amount of 0.0001 to 0.1 wt% based on the total weight of the composition, sunflower oil may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition, moringa oil may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition, castor oil may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition, and argan oil may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition. The tocopherol may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition, and the jojoba oil may be included in an amount of 0.00001 to 0.01 wt% based on the total weight of the composition.

[0032] In the present invention, "anti-inflammation" means suppressing inflammation, and inflammation is one of the defense mechanisms of living tissues against certain stimuli such as pathogens or microorganisms invading from the outside, and refers to a complex lesion that causes tissue degeneration, circulatory disorder, exudation, and tissue proliferation. Examples of skin diseases related to inflammation include, but are not limited to, atopic dermatitis, psoriasis, erythematous diseases triggered by radiation, chemicals, burns, acid burns, bullous dermatoses, lichen planus-like diseases, allergic pruritus, seborrheic eczema, acne rosacea, pemphigus vulgaris, erythema multiforme exudative, erythema nodosum, balanitis, vulvitis, inflammatory hair loss such as alopecia areata, and cutaneous T-cell lymphoma.

[0033] In the present invention, “moisturizing” means increasing moisture content in the skin and maintaining a moist state.

[0034] In the present invention, "skin regeneration" refers to the process of skin tissue recovery from damage caused by external or internal factors. Damage caused by external factors may include ultraviolet rays, external pollutants, wounds, trauma, and the like, while damage caused by internal factors may include stress.

[0035] According to one embodiment of the present invention, a 10-component complex including patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil significantly inhibited the production of prostaglandin E2 (PGE2), IL-6, and IL-8 in cells induced with inflammation by LPS treatment, confirming its anti-inflammation effect (Example 1).

[0036] According to one embodiment of the present invention, it was confirmed that a 10-component complex including patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil significantly increased the amount of hyaluronic acid (HA) production and the mRNA expression of HAS2, a hyaluronic acid synthesis factor, in human-derived keratinocytes, thereby being effective in moisturizing (Example 2).

[0037] According to one embodiment of the present invention, a 10-component complex including patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil significantly increased cell proliferation rate in human-derived keratinocytes and healed wounds over a wider area, confirming its effectiveness in skin regeneration (Example 3).

[0038] This means that even though there were ingredients that had minimal effects or were less effective than the untreated group when the above 10 ingredients were used alone, when all 10 ingredients were included, they could show significantly increased effects in anti-inflammation, moisturizing, and skin regeneration.

[0039] The cosmetic composition according to the present invention can be provided in any formulation suitable for topical application, and can be prepared in a formulation selected from the group consisting of solutions, external ointments, creams, foams, nourishing toners, emollients, packs, emollients, emulsions, makeup bases, essences, soaps, liquid cleansers, bath products, sunscreen creams, sun oils, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing oils, powder foundations, emulsion foundations, wax foundations, patches, and sprays, but is not limited thereto.

[0040] In addition, the cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers that are incorporated into general skin cosmetics, and may appropriately incorporate conventional ingredients such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., but is not limited thereto. The carrier may be introduced in an amount generally used in the fields of cosmetics or dermatology.

[0041] The present invention also provides a pharmaceutical composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0042] In the present invention, "quasi-drug" means a product that is used for the purpose of diagnosing, treating, improving, alleviating, managing or preventing a disease of humans or animals, and has a milder effect than a pharmaceutical product. For example, according to the Pharmaceutical Affairs Act, a quasi-drug is a product used for the purpose of treating or preventing a disease of humans or animals, or a product that has a mild effect on the human body or does not act directly. For example, it may be selected from the group consisting of, but is not limited to, disinfectant cleaners, detergents, kitchen detergents, cleaning detergents, wet tissues, detergents, soaps, hand washes, hair cleansers, hair softeners, and ointments.

[0043] In the pharmaceutical composition of the present invention, the patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil, anti-inflammation, skin moisturizing, and skin regeneration are as described above in the cosmetic composition.

[0044] In the present invention, the formulation method, dosage, and additional additive ingredients of the quasi-drug can be appropriately selected from techniques known in the art.

[0045] In another aspect, the present invention provides an anti-inflammatory, moisturizing or skin regeneration method comprising a step of applying or administering to the skin of a subject a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

[0046] The above composition may be a cosmetic composition or a pharmaceutical composition.

[0047] In the present invention, the term "subject" may mean any mammal, including humans, in need of anti-inflammation, moisturizing, or improving skin regeneration.

[0048] In the present invention, the term "application" or "administration" means contacting or providing the composition according to the present invention to the skin of a subject, and may be applied or administered using a general route that reaches the skin or is absorbed into the skin.

[0049] In another aspect, the present invention provides the use of a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil for preparing an anti-inflammatory, moisturizing, or skin regeneration composition.

[0050] The above composition may be a cosmetic composition or a pharmaceutical composition.

[0051] In the method and use of the present invention, the patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, jojoba oil, anti-inflammation, skin moisturizing, skin regeneration, cosmetics, and quasi-drugs are as described above.

[0052] Each of the above-mentioned ingredients included in the cosmetic composition according to the present invention may be included in the cosmetic composition of the present invention within a range that preferably does not exceed the maximum usage amount specified in the regulations related to “cosmetic use and approval” stipulated by the government of each country.

[0053] Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

[0054] Example

[0055] Example 1. Skin anti-inflammatory evaluation

[0056] Example 1-1. Evaluation of PGE2 production inhibition by single or combined treatment with 10 different agents.

[0057] The inhibitory efficacy of prostaglandin E2 (PGE2) production was evaluated by treatment with 10 types of complexes: 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil.

[0058] First, THP-1 cell line (human monocytic cell line) was cultured at 3 x 10 in RPMI1640 (Roswell Park Memorial Institute 1640 medium) containing 10% FBS (Fetal Bovine Serum). 5 The cells were seeded at a concentration of cells / ml and cultured in a 24-well plate at 37°C and 5% CO2 for 24 hours. Then, 1 μg / ml of LPS, an inflammation-inducing stimulus, and 10 types of samples, including patchouli extract, or a combination thereof, were treated at different concentrations and cultured for an additional 24 hours, and then a PGE2 ELISA assay kit (Thermo SCIENTIFIC) was performed. The PGE2 ELISA assay measured the amount of PGE2 according to the treatment of the 10 types alone or in combination using the supernatant according to the manufacturer's instructions.

[0059] PGE2 amount (% of LPS treatment group) Increase rate (%) Untreated control group 14.04 ± 1.96 LPS (1 ppm) treatment group 100 ± 17.07 ###0Glycerin (70 ppm) 130.80 ± 13.44 30.80Macadamia Oil (10 ppm) 129.97 ± 21.24 29.97Shea Butter 25 ppm 132.53 ± 18.0 332.53Sunflower Oil 1 ppm 107.71 ± 16.68 7.71Moringa Oil 2 ppm 120.50 ± 16.0 320.50Castor Oil 2 ppm 122.17 ± 17.6 222.17Argan Oil 2 ppm 119.98 ± 19.70 19.89Tocopherol 1 ppm 112.71 ± 17.5 112.71Jojoba Oil 2 ppm 104.50 ± 26.574.50 Patchouli Extract 100ppm 92.25 ± 11.98-7.75 10 Species Complex 59.00 ± 8.14 ***-41.12

[0060] # vs control group, * vs LPS-treated group,

[0061] ### or *** p < 0.001

[0062] As shown in Table 1, when patchouli extract was treated to cells in which inflammation was induced by LPS treatment (LPS treatment group), the amount of PGE2 slightly decreased, but in the group treated with glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil alone, the amount of PGE2 increased further. However, when a complex of 10 of the above ingredients was treated, the amount of PGE2 decreased significantly. In particular, the sum of the increase and decrease rates of the single treatment groups for PGE2 was 173.03%, whereas the increase and decrease rate of the 10 complex treatment groups was -41.12%, indicating that the amount of PGE2 was significantly reduced when the 10 complexes were treated.

[0063]

[0064] Example 1-2. Evaluation of inhibition of IL-6 and IL-8 production by treatment with 10 types of agents alone or in combination.

[0065] The inhibitory efficacy of IL-6 and IL-8 production was evaluated by treatment with 10 types of complexes including 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil.

[0066] IL-6 (% of LPS treatment group) Increase rate (%) IL-8 (% of LPS treatment group) Increase rate (%) Untreated control group 0.23 ± 0.15 14.09 ± 5.92 LPS (1 ppm) treatment group 100 ± 20.81 ### 0100.00 ± 5.14 ### 0Glycerin 70 ppm97.12 ± 14.92-2.8898.77 ± 2.21-1.23Macadamia Oil 10 ppm127.84 ± 24.89 **27.85103.82 ± 6.263.82Shea Butter 25 ppm157.20 ± 16.75 ***57.20103.26 ± 9.883.26Sunflower Oil 1 ppm88.42 ± 32.77-11.57111.39 ± 10.9911.39Moringa Oil 2 ppm112.95 ± 35.6312.95157.85 ± 68.37*57.85Castor Oil 2 ppm103.27 ± 27.763.2788.33 ± 5.10*-11.67Argan oil 2ppm176.51± 81.4676.51173.48 ± 39.4573.48Tocopherol 1ppm95.48 ± 12.58-4.5292.82 ± 3.49-7.18Jojoba oil 2ppm92.46 ± 12.97-7.54101.66 ± 28.151.66Patchouli extract 100ppm79.09 ± 13.70-20.9184.15 ± 6.21*-15.8510 species complex42.47 ± 5.30 *-57.5325.35 ± 1.54***-74.65

[0067] # vs control group, * vs LPS-treated group,

[0068] * p < 0.05, ** p < 0.01, ### or *** p < 0.001

[0069] As shown in Table 2, the amount of IL-6 increased more in the group treated with macadamia oil, shea butter, moringa oil, castor oil, and argan oil alone in cells in which inflammation was induced by LPS treatment (LPS treatment group), and the amount of IL-8 increased more in the group treated with macadamia oil, shea butter, sunflower oil, moringa oil, and jojoba oil alone. However, when the 10-type complex was treated, the amounts of IL-6 and IL-8 decreased significantly. In particular, the sum of the increase / decrease rates of the single treatment group for IL-6 was 130.36%, while the increase / decrease rate of the 10-complex treatment group was -57.53%, and the sum of the increase / decrease rates of the single treatment group for IL-8 was 42.11%, while the increase / decrease rate of the 10-complex treatment group was -74.65%, indicating that the amount of IL-6 and IL-8 was significantly reduced when the 10-complex was treated.

[0070] Accordingly, when the ingredients are treated alone, they may not exhibit anti-inflammatory effects or may worsen them, but when treated as a 10-item complex, they can exhibit effective anti-inflammatory effects.

[0071] Example 2. Skin moisturizing evaluation

[0072] Example 2-1. Evaluation of hyaluronic acid (HA) production according to single or combined treatment with 10 types.

[0073] The amount of hyaluronic acid, a moisturizing factor, produced was confirmed in a 10-complex of patchouli extract 100 ppm, glycerin 70 ppm, macadamia oil 10 ppm, shea butter 25 ppm, sunflower oil 1 ppm, moringa oil 2 ppm, castor oil 2 ppm, argan oil 2 ppm, tocopherol 1 ppm, and jojoba oil 2 ppm.

[0074] HaCaT cells (human keratinocytes) were seeded at 1 x 10 in a 24-well plate. 5After dispensing at a concentration of 10 / well, the cells were cultured for 24 hours in a basic medium containing a mixture of DMEM (Dulbecco's Modified Eagle's Medium) and FBS (Fetal Bovine Serum). After removing the medium, the cells were washed with PBS (Phosphate Buffer Saline), and each of the 10 samples including patchouli extract or a combination thereof was treated at different concentrations in serum-free DMEM. After 24 hours of culture, the supernatant was collected, and the produced hyaluronic acid (HA) content was measured using a Hyaluronan DuoSet ELISA kit (R&D Systems, MN, USA) according to the method provided by the manufacturer. The amount of hyaluronic acid produced after treatment with the 10 raw materials alone or in combination was expressed as a percentage (%) compared to the control group.

[0075] HA (% of control) Increase (%) Untreated control 100 ± 9.0 50 Glycerin 70 ppm 110 ± 13.9 4 10.30 Macadamia oil 10 ppm 8 1.72 ± 18.58 - 18.28 Shea butter 25 ppm 7 2.87 ± 13.06 * - 27.13 Sunflower oil 1 ppm 10 1.18 ± 13.8 5 1.18 Moringa oil 2 ppm 12 9.68 ± 19.2 7 29.68 Castor oil 2 ppm 115.26 ± 6.7 1 15.26 Argan oil 2 ppm 9 8.91 ± 9.66 - 1.08 5 Tocopherol 1 ppm 9 8.44 ± 5.85 - 1.56 Jojoba oil 2ppm93.49 ± 6.79-6.51Patchouli extract100ppm120.41 ± 8.14 *20.4110 species complex263.67 ± 44.38 *163.67

[0076] * p <0.05

[0077] As shown in Table 3, a slight increase in hyaluronic acid production was confirmed in patchouli extract, but in the group treated with macadamia oil, shea butter, argan oil, tocopherol, and jojoba oil alone, the production of hyaluronic acid decreased compared to the untreated control group. However, when treated with the 10-type complex, the production of hyaluronic acid increased significantly. In particular, the combined increase / decrease rates of the single treatment groups were -22.265%, whereas the increase / decrease rate of the 10-type complex treatment group was 163.67%, indicating that the production of hyaluronic acid was higher when treated with the 10-type complex.

[0078] Accordingly, even ingredients that do not affect the production of hyaluronic acid or reduce the production of hyaluronic acid when treated alone can significantly increase the production of hyaluronic acid when treated as a 10-component complex, thereby exhibiting an effective moisturizing effect.

[0079] Example 2-2. Evaluation of hyaluronic acid synthase (HAS2) gene expression according to single or combined treatment with 10 types.

[0080] The expression level of the hyaluronic acid synthase (HAS2) gene, a moisturizing factor, was confirmed for 10 types of complexes: 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil.

[0081] Human keratinocytes (HaCaT) were seeded at 2*10 in a 6-well plate. 5Each well was dispensed and cultured in a CO2 incubator for 24 hours. The 10 types, either alone or in combination, were added to the culture medium and cultured in an incubator for 24 hours. RNA was extracted according to the protocol using an RNA-spin Total RNA Extraction Kit (Intron Biotechnology, Korea), and real-time qPCR was performed using Taqman probes (ThermoFisher scientific) to confirm the level of HAS2 expression by the 10 types, either alone or in combination.

[0082] HAS2 expression (% of control) Increase rate (%) Untreated control group 100 ± 6.5 70 Glycerin 70 ppm 103.69 ± 18.1 73.54 Macadamia oil 10 ppm 47.66 ± 2.15 *-52.48 Shea butter 25 ppm 65.33 ± 4.76 **-34.81 Sunflower oil 1 ppm 113.49 ± 28.6 513.34 Moringa oil 2 ppm 92.50 ± 14.27 *-7.64 Castor oil 2 ppm 94.94 ± 9.64 *-5.21 Argan oil 2 ppm 95.94 ± 8.11 **-4.21 Tocopherol 1 ppm 113.49 ± 28.65 *13.34 Jojoba oil 2ppm 84.11 ± 2.09 **-16.04 Patchouli extract 100ppm 123.81 ± 6.74 **23.66 10 species complex 205.52 ± 22.68 **105.38

[0083] * p <0.05, ** p<0.01

[0084] As shown in Table 4, a slight increase was confirmed in the group treated with tocopherol and patchouli extract alone, but in the group treated with macadamia oil, shea butter, moringa oil, castor oil, argan oil, and jojoba oil alone, the HAS2 gene expression level decreased more than the untreated control group. However, when treated with the 10 types of complexes, the HAS2 gene expression level increased significantly. In particular, the sum of the increase / decrease rates of the single treatment groups was -66.51%, whereas the increase / decrease rate of the 10 types of complex treatment groups was 105.38%, indicating that the HAS2 gene expression level was higher when treated with the 10 types of complexes.

[0085] Accordingly, when treated alone, the moisturizing effect may be minimal or worse, but when treated as a 10-type complex, it can exhibit an effective moisturizing effect.

[0086] Example 3. Evaluation of skin regeneration efficacy

[0087] Example 3-1. Evaluation of keratinocyte growth according to single or combined treatment with 10 types of substances.

[0088] The cell proliferation rate of keratinocytes (HaCaT) was confirmed with 10 types of complexes including 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil.

[0089] Human skin-derived epidermal keratinocytes (HaCaT) were cultured using DMEM medium containing 10% FBS, and then seeded at 1X10 in a 96-well plate (96 well plate, Corning). 4 Cells were dispensed per well and cultured for 24 hours in a 37°C, 5% CO2 incubator.

[0090] After culturing for 24 hours with the 10 components alone or in combination at the concentrations shown in Table 5, CCK-8 (Cell Counting Kit-8, Dojindo Laboratory) and DMEM were mixed at a ratio of 1:10 and treated with 100 μL per well. After reacting in an incubator for 30 minutes, the absorbance was measured at 450 nm and converted to a percentage. The results are shown in Table 5. The untreated control group was not treated with any of the 10 components.

[0091] Cell proliferation rate (% of control group) Increase rate (%) Untreated control group 100 ± 19.460 Glycerin 70 ppm 107.39 ± 13.73 7.39 3308 Macadamia oil 10 ppm 95.89 ± 8.26-4.10 739 Shea butter 25 ppm 103.20 ± 5.66 3.20 3343 Sunflower oil 1 ppm 107.39 ± 10.49 7.39 3308 Moringa oil 2 ppm 109.32 ± 17.28 9.31 677 Castor oil 2 ppm 103.07 ± 12.80 3.065 518 Argan oil 2 ppm 98.93 ± 3.67-1.06 778 Tocopherol 1ppm98.18 ± 12.22-1.82328Jojoba oil2ppm86.48 ± 6.92-13.5243Patchouli extract100ppm113.59 ± 3.0313.5853510 species complex134.68 ± 5.73**34.6825

[0092] ** p<0.01

[0093] As shown in Table 5, although not significant, a slight increase in cell proliferation rate was confirmed in the group treated with glycerin, shea butter, sunflower oil, moringa oil, castor oil, and patchouli extract alone, but in the group treated with macadamia oil, argan oil, tocopherol, and jojoba oil alone, the cell proliferation rate of keratinocytes decreased more than in the untreated control group. However, when treated with the 10-type complex, the cell proliferation rate of keratinocytes increased significantly. In particular, the combined increase rate of the single treatment group was 23.43485%, whereas the increase rate of the 10-type complex treatment group was 34.6825%, indicating that the cell proliferation rate was higher when treated with the 10-type complex.

[0094] Accordingly, when treated alone, it may not affect the cell proliferation rate of keratinocytes or may worsen it, but when treated as a 10-type complex, it can significantly increase the cell proliferation rate of keratinocytes, resulting in an effective skin regeneration effect.

[0095] Example 3-2. Increased in vitro wound healing efficacy

[0096] To create a wound healing model using HaCaT cells, HaCaT cells 2.0 Х 10 4 Cells were placed in each well of a Culture-Insert 2 Well 24 plate (Ibidi GmbH, Grδfelfing, Germany) and cultured for 24 hours. After culture, the inserts were removed, and 100 ppm of patchouli extract, 70 ppm of glycerin, 10 ppm of macadamia oil, 25 ppm of shea butter, 1 ppm of sunflower oil, 2 ppm of moringa oil, 2 ppm of castor oil, 2 ppm of argan oil, 1 ppm of tocopherol, and 2 ppm of jojoba oil were treated alone or in combination to the cultured HaCaT wound healing model. The results confirmed by optical microscopy 18 hours later are shown in Fig. 1.

[0097] From Fig. 1, compared to the untreated control group, the group treated with each component alone showed minimal or slight wound healing, but when the 10-component complex was treated, it was confirmed that the wound healed over a significantly wider area.

Claims

1. A cosmetic composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

2. In paragraph 1, A cosmetic composition wherein the above patchouli extract is extracted with a solvent selected from the group consisting of water, lower alcohols having C1 to C4, or mixtures thereof.

3. In paragraph 1, A cosmetic composition wherein the weight ratio of patchouli extract: glycerin: macadamia oil: shea butter: sunflower oil: moringa oil: castor oil: argan oil: tocopherol: jojoba oil is 1:0.5~1:0.05~0.3:0.05~0.4:0.005~0.03:0.005~0.03:0.005~0.03:0.005~0.03:0.005~0.03:0.005~0.03:0.005~0.

03.

4. In paragraph 1, The above cosmetic composition is an anti-inflammatory cosmetic composition.

5. In paragraph 1, The above cosmetic composition is a moisturizing cosmetic composition.

6. In paragraph 1, The above cosmetic composition is a cosmetic composition for skin regeneration.

7. A pharmaceutical composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

8. In paragraph 7, The above pharmaceutical composition is an anti-inflammatory, moisturizing, or skin regeneration pharmaceutical composition.

9. An anti-inflammatory method comprising a step of applying or administering to the skin of a subject a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

10. A method for moisturizing skin, comprising a step of applying or administering to the skin of a subject a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

11. A method for skin regeneration comprising a step of applying or administering to the skin of a subject a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil.

12. Use of a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil for preparing an anti-inflammatory composition.

13. Use of a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil for producing a skin moisturizing composition.

14. Use of a composition comprising patchouli extract, glycerin, macadamia oil, shea butter, sunflower oil, moringa oil, castor oil, argan oil, tocopherol, and jojoba oil for preparing a composition for skin regeneration.

Images